MD07G1164800.v1.1
NAC Family

inactive purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007460
Physical Location & Seq
Reverse (-)
23966820 .. 23969514
2695 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1164800.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGAAGAGGGACAGGGAGGTGCTAAGGAGTCCATTTTTAGCGGCGGTTGTGGCGGCGGCGCTGCTGCCGATATGGGTTTTGGCTGCCGCAAAACAACAGAACCACCACCTCCAACGAGGAATTGGGGATAAGAAGCTGACGTTTGGGACAGATGGGCAGTTTAAGATTCTGCAAGTGGCGGATATGCACTACGGCAATGGCAAGACTACGCCTTGTAAGGATGTGCTGCCGTCCCAGTTTGCAACTTGCTCTGACCTCAACACCACCGCCTTTGTACGCCGCATGATCGAGGCGGAGAAGCCGAATCTCATCGTCTTCACCGGAGATAACATATACGGGTTTGATGTCACGAATGCTGCTAAATCTTTGAACGAGGCGTTCGCCCCAGCAATCGCGTCCAACATCCCGTGGGCAGCTGTGTTGGGGAACCATGATCAGCAATCCGACCTCTCGAGGGAAGGGGTAATGAAACACATAGTTGGGTTGAAGAACACTCTGGCTCAAGTTAATCCTTTAGGCCAAGATGCTATTGATGGTTTTGGGAACTATAACCTAGAAGTCGCCGGAGTTGAGGGCTTTAGTTTTGAAAACAAATCGGTTCTCAATCTTTACTTCCTTGACAGCGGAGATTACTCTACGGTTCCCTCCATAGGTCGCTATGGTTGGATCAAACCCTCCCAACAATATTGGTTTGAACGAACTTCTGCAAAGCTTCAGAAAGCATACATGAGCAAGCCCCTACCCCAGAAAGCGCCTGCGCCAGGGATCGCATACTTTCACATACCATTGCCGGAATTTGCTAGTTTTGACTCATCAAACTTTACAGGAGTGAAACAAGAAGGCATTAGCTCTGCTTCTGTGAACTCGGGCTTCTTCACAACCATGGTTGCAGCAGGAGATGTGAAGGCAGTGTTCATAGGCCATGATCACCTCAATGACTTCTGTGGGGAGCTAAGTGGTATAAACCTTTGTTATGCTGGGGGATTTGGATACCATGCTTATGGGAAGGCTGGATGGGATAGGAGAGCAAGGGTGGTGGTAGCAAACTTAGAGAAAACGGTTAAGGGAGGTTGGGGAGCGGTCAAGTCAATCAAAACATGGAAACGCCTTGATGATGAGCACCTCACTGCTATCGATGGTCAGGTTCTCTGGAGCAAGAGCTCTGTGGGTATGCGTAGAAAGAAACCTGTTGGCGGTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

401

Amino Acids

43.65

Weight (kDa)

9.04

Isoelectric Point (pI)

38.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 54 - 310 1.1e-13 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1079
AccII CGCG 1 cut(s) 395
AclWI GGATC 2 cut(s) 674, 773
AcsI RAATTY 1 cut(s) 794
AcuI CTGAAG 1 cut(s) 698
AfaI GTAC 1 cut(s) 276
AfiI CCNNNNNNNGG 4 cut(s) 454, 650, 761, 1193
AgsI TTSAA 4 cut(s) 370, 487, 587, 695
AjnI CCWGG 1 cut(s) 760
AluBI AGCT 6 cut(s) 136, 416, 712, 849, 952, 1161
AluI AGCT 6 cut(s) 136, 416, 712, 849, 952, 1161
Alw21I GWGCWC 2 cut(s) 1122, 1163
AlwI GGATC 2 cut(s) 674, 773
Ama87I CYCGRG 2 cut(s) 451, 865
AoxI GGCC 2 cut(s) 517, 919
ApeKI GCWGC 7 cut(s) 61, 64, 83, 226, 356, 413, 890
ApoI RAATTY 1 cut(s) 794
AspLEI GCGC 3 cut(s) 61, 754, 760
AsuHPI GGTGA 2 cut(s) 310, 920
AvaI CYCGRG 2 cut(s) 451, 865
BanII GRGCYC 1 cut(s) 1163
BbsI GAAGAC 1 cut(s) 307
Bbv12I GWGCWC 2 cut(s) 1122, 1163
BbvI GCAGC 7 cut(s) 48, 51, 70, 213, 343, 425, 902
BccI CCATC 4 cut(s) 146, 527, 1008, 1130
BceAI ACGGC 2 cut(s) 208, 214
BciT130I CCWGG 1 cut(s) 762
BciVI GTATCC 1 cut(s) 983
BclI TGATCA 2 cut(s) 433, 925
BfaI CTAG 2 cut(s) 554, 801
BfoI RGCGCY 2 cut(s) 62, 755
BfuI GTATCC 1 cut(s) 983
Bme1390I CCNGG 1 cut(s) 762
BmeT110I CYCGRG 2 cut(s) 451, 865
BmiI GGNNCC 2 cut(s) 428, 642
BmrFI CCNGG 1 cut(s) 762
BmrI ACTGGG 1 cut(s) 229
BmsI GCATC 1 cut(s) 514
BmuI ACTGGG 1 cut(s) 229
BpiI GAAGAC 1 cut(s) 307
BpmI CTGGAG 1 cut(s) 1171
Bpu10I CCTNAGC 1 cut(s) 23
BpuEI CTTGAG 1 cut(s) 486
Bsa29I ATCGAT 1 cut(s) 1134
BsaJI CCNNGG 3 cut(s) 407, 761, 882
BsaWI WCCGGW 1 cut(s) 320
Bsc4I CCNNNNNNNGG 4 cut(s) 454, 650, 761, 1193
Bse1I ACTGG 1 cut(s) 235
Bse3DI GCAATG 2 cut(s) 202, 785
BseBI CCWGG 1 cut(s) 762
BseCI ATCGAT 1 cut(s) 1134
BseDI CCNNGG 3 cut(s) 407, 761, 882
BseGI GGATG 3 cut(s) 226, 402, 1019
BseLI CCNNNNNNNGG 4 cut(s) 454, 650, 761, 1193
BseMI GCAATG 2 cut(s) 202, 785
BseNI ACTGG 1 cut(s) 235
BseXI GCAGC 7 cut(s) 48, 51, 70, 213, 343, 425, 902
BseYI CCCAGC 2 cut(s) 385, 977
Bsh1236I CGCG 1 cut(s) 395
BshFI GGCC 2 cut(s) 519, 921
BshVI ATCGAT 1 cut(s) 1134
BsiHKAI GWGCWC 2 cut(s) 1122, 1163
BsiHKCI CYCGRG 2 cut(s) 451, 865
BsiSI CCGG 3 cut(s) 321, 564, 791
BslFI GGGAC 3 cut(s) 23, 160, 217
BslI CCNNNNNNNGG 4 cut(s) 454, 650, 761, 1193
BsmFI GGGAC 3 cut(s) 23, 160, 217
BsmI GAATGC 1 cut(s) 358
BsnI GGCC 2 cut(s) 519, 921
BsoBI CYCGRG 2 cut(s) 451, 865
Bsp1286I GDGCHC 2 cut(s) 1122, 1163
Bsp143I GATC 5 cut(s) 285, 433, 666, 765, 925
Bsp19I CCATGG 1 cut(s) 882
BspANI GGCC 2 cut(s) 519, 921
BspDI ATCGAT 1 cut(s) 1134
BspFNI CGCG 1 cut(s) 395
BspLI GGNNCC 2 cut(s) 428, 642
BspPI GGATC 2 cut(s) 674, 773
BsrBI CCGCTC 1 cut(s) 1079
BsrDI GCAATG 2 cut(s) 202, 785
BsrI ACTGG 1 cut(s) 235
BssECI CCNNGG 3 cut(s) 407, 761, 882
BssMI GATC 5 cut(s) 285, 433, 666, 765, 925
BssT1I CCWWGG 1 cut(s) 882
Bst2UI CCWGG 1 cut(s) 762
Bst4CI ACNGT 2 cut(s) 640, 1060
BstC8I GCNNGC 2 cut(s) 734, 756
BstDEI CTNAG 3 cut(s) 23, 953, 1048
BstDSI CCRYGG 2 cut(s) 407, 882
BstENI CCTNNNNNAGG 1 cut(s) 759
BstF5I GGATG 3 cut(s) 226, 402, 1019
BstFNI CGCG 1 cut(s) 395
BstH2I RGCGCY 2 cut(s) 62, 755
BstHHI GCGC 3 cut(s) 61, 754, 760
BstKTI GATC 5 cut(s) 288, 436, 669, 768, 928
BstMBI GATC 5 cut(s) 285, 433, 666, 765, 925
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNI CCWGG 1 cut(s) 762
BstSCI CCNGG 1 cut(s) 760
BstUI CGCG 1 cut(s) 395
BstV1I GCAGC 7 cut(s) 48, 51, 70, 213, 343, 425, 902
BstV2I GAAGAC 1 cut(s) 307
BstXI CCANNNNNNTGG 1 cut(s) 1001
Bsu15I ATCGAT 1 cut(s) 1134
BsuI GTATCC 1 cut(s) 983
BsuRI GGCC 2 cut(s) 519, 921
BsuTUI ATCGAT 1 cut(s) 1134
BtgI CCRYGG 2 cut(s) 407, 882
BtsCI GGATG 3 cut(s) 226, 402, 1019
BtsI GCAGTG 2 cut(s) 915, 1125
BtsIMutI CAGTG 2 cut(s) 915, 1125
Cac8I GCNNGC 2 cut(s) 734, 756
CfoI GCGC 3 cut(s) 61, 754, 760
ClaI ATCGAT 1 cut(s) 1134
CseI GACGC 1 cut(s) 384
Csp6I GTAC 1 cut(s) 275
CspCI CAANNNNNGTGG 4 cut(s) 253, 288, 1017, 1052
CviAII CATG 7 cut(s) 283, 431, 727, 883, 923, 995, 1098
CviQI GTAC 1 cut(s) 275
DdeI CTNAG 3 cut(s) 23, 953, 1048
DpnI GATC 5 cut(s) 287, 435, 668, 767, 927
DpnII GATC 5 cut(s) 285, 433, 666, 765, 925
EciI GGCGGA 2 cut(s) 194, 308
Ecl136II GAGCTC 1 cut(s) 1161
Eco130I CCWWGG 1 cut(s) 882
Eco24I GRGCYC 1 cut(s) 1163
Eco53kI GAGCTC 1 cut(s) 1161
Eco57I CTGAAG 1 cut(s) 698
Eco88I CYCGRG 2 cut(s) 451, 865
EcoICRI GAGCTC 1 cut(s) 1161
EcoNI CCTNNNNNAGG 1 cut(s) 759
EcoRII CCWGG 1 cut(s) 760
EcoT14I CCWWGG 1 cut(s) 882
EcoT38I GRGCYC 1 cut(s) 1163
ErhI CCWWGG 1 cut(s) 882
FaeI CATG 7 cut(s) 286, 434, 730, 886, 926, 998, 1101
FaqI GGGAC 3 cut(s) 23, 160, 217
FatI CATG 7 cut(s) 282, 430, 726, 882, 922, 994, 1097
FbaI TGATCA 2 cut(s) 433, 925
FokI GGATG 3 cut(s) 233, 389, 1026
FriOI GRGCYC 1 cut(s) 1163
FspBI CTAG 2 cut(s) 554, 801
GlaI GCGC 3 cut(s) 60, 753, 759
GsaI CCCAGC 2 cut(s) 389, 981
GsuI CTGGAG 1 cut(s) 1171
HaeII RGCGCY 2 cut(s) 62, 755
HaeIII GGCC 2 cut(s) 519, 921
HapII CCGG 3 cut(s) 321, 564, 791
HgaI GACGC 1 cut(s) 384
HhaI GCGC 3 cut(s) 61, 754, 760
Hin1II CATG 7 cut(s) 286, 434, 730, 886, 926, 998, 1101
Hin6I GCGC 3 cut(s) 59, 752, 758
HinP1I GCGC 3 cut(s) 59, 752, 758
HindIII AAGCTT 1 cut(s) 710
HinfI GANTC 4 cut(s) 28, 166, 304, 809
HpaII CCGG 3 cut(s) 321, 564, 791
HphI GGTGA 2 cut(s) 310, 920
Hpy166II GTNNAC 1 cut(s) 862
Hpy188I TCNGA 3 cut(s) 253, 445, 717
Hpy188III TCNNGA 3 cut(s) 349, 451, 1150
Hpy8I GTNNAC 1 cut(s) 862
HpyAV CCTTC 4 cut(s) 452, 833, 898, 1000
HpyCH4III ACNGT 2 cut(s) 640, 1060
HpyCH4IV ACGT 1 cut(s) 140
HpyCH4V TGCA 5 cut(s) 172, 187, 242, 707, 890
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 3 cut(s) 23, 953, 1048
HpySE526I ACGT 1 cut(s) 140
Hsp92II CATG 7 cut(s) 286, 434, 730, 886, 926, 998, 1101
HspAI GCGC 3 cut(s) 59, 752, 758
Ksp22I TGATCA 2 cut(s) 433, 925
Kzo9I GATC 5 cut(s) 285, 433, 666, 765, 925
LmnI GCTCC 3 cut(s) 949, 1076, 1152
Lsp1109I GCAGC 7 cut(s) 48, 51, 70, 213, 343, 425, 902
LweI GCATC 1 cut(s) 514
MaeI CTAG 2 cut(s) 554, 801
MaeII ACGT 1 cut(s) 140
MaeIII GTNAC 2 cut(s) 346, 1196
MalI GATC 5 cut(s) 287, 435, 668, 767, 927
MbiI CCGCTC 1 cut(s) 1079
MboI GATC 5 cut(s) 285, 433, 666, 765, 925
MboII GAAGA 4 cut(s) 16, 307, 499, 865
MhlI GDGCHC 2 cut(s) 1122, 1163
MluCI AATT 2 cut(s) 120, 794
MlyI GAGTC 2 cut(s) 37, 803
MmeI TCCRAC 4 cut(s) 136, 423, 468, 644
MseI TTAA 3 cut(s) 162, 507, 1062
MslI CAYNNNNRTG 3 cut(s) 881, 933, 999
MspA1I CMGCKG 2 cut(s) 416, 624
MspI CCGG 3 cut(s) 321, 564, 791
MspR9I CCNGG 1 cut(s) 762
Mva1269I GAATGC 1 cut(s) 358
MvaI CCWGG 1 cut(s) 762
MvnI CGCG 1 cut(s) 395
MwoI GCNNNNNNNGC 1 cut(s) 50
NcoI CCATGG 1 cut(s) 882
NdeII GATC 5 cut(s) 285, 433, 666, 765, 925
NlaIII CATG 7 cut(s) 286, 434, 730, 886, 926, 998, 1101
NlaIV GGNNCC 2 cut(s) 428, 642
NmuCI GTSAC 1 cut(s) 346
PaeR7I CTCGAG 1 cut(s) 451
PctI GAATGC 1 cut(s) 358
PfeI GAWTC 2 cut(s) 166, 304
PleI GAGTC 2 cut(s) 36, 803
PpsI GAGTC 2 cut(s) 36, 803
Psp124BI GAGCTC 1 cut(s) 1163
Psp6I CCWGG 1 cut(s) 760
PspFI CCCAGC 2 cut(s) 385, 977
PspGI CCWGG 1 cut(s) 760
PspN4I GGNNCC 2 cut(s) 428, 642
PvuII CAGCTG 1 cut(s) 416
RsaI GTAC 1 cut(s) 276
RsaNI GTAC 1 cut(s) 275
RseI CAYNNNNRTG 3 cut(s) 881, 933, 999
SacI GAGCTC 1 cut(s) 1163
SaqAI TTAA 3 cut(s) 162, 507, 1062
Sau3AI GATC 5 cut(s) 285, 433, 666, 765, 925
SchI GAGTC 2 cut(s) 37, 803
ScrFI CCNGG 1 cut(s) 762
SduI GDGCHC 2 cut(s) 1122, 1163
SfaNI GCATC 1 cut(s) 514
Sfr274I CTCGAG 1 cut(s) 451
SlaI CTCGAG 1 cut(s) 451
SmiMI CAYNNNNRTG 3 cut(s) 881, 933, 999
SmlI CTYRAG 2 cut(s) 451, 501
SmoI CTYRAG 2 cut(s) 451, 501
Sse9I AATT 2 cut(s) 120, 794
SspI AATATT 1 cut(s) 686
SspMI CTAG 2 cut(s) 554, 801
SstI GAGCTC 1 cut(s) 1163
StyD4I CCNGG 1 cut(s) 760
StyI CCWWGG 1 cut(s) 882
TaaI ACNGT 2 cut(s) 640, 1060
TaiI ACGT 1 cut(s) 143
TaqI TCGA 3 cut(s) 288, 452, 1134
TasI AATT 2 cut(s) 120, 794
TauI GCSGC 5 cut(s) 44, 56, 59, 89, 282
TfiI GAWTC 2 cut(s) 166, 304
Tru1I TTAA 3 cut(s) 162, 507, 1062
Tru9I TTAA 3 cut(s) 162, 507, 1062
TscAI CASTG 2 cut(s) 915, 1132
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 7 cut(s) 61, 64, 83, 226, 356, 413, 890
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 3 cut(s) 17, 482, 904
TspRI CASTG 2 cut(s) 915, 1132
XagI CCTNNNNNAGG 1 cut(s) 759
XapI RAATTY 1 cut(s) 794
XcmI CCANNNNNNNNNTGG 1 cut(s) 119
XhoI CTCGAG 1 cut(s) 451
XspI CTAG 2 cut(s) 554, 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.