MD01G1098300.v1.1
NAC Family

inactive purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007460
Physical Location & Seq
Reverse (-)
21210868 .. 21214629
3762 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1098300.v1.1.491

Sequence Viewer

Length: 1218 bp
ATGAGTGGTACAGTGACAGTGAGGATGAAGAGGGACATGGAGGTGCTAAGGAGTCCATTTTTAGTGGCGGCGGTGGTGGCGCTGCTTCCTATATGGGGTATGGCCGCCGCAGAACAACAGCACCACCACCAAGGTAAAGGTGGGGAGAAGAAGCTGCGGTTTGGGACAGATGGGCAGTTTAAGATTCTGCAAGTGGCGGATATGCACTATGCCGATGGCAAGACCACGCCCTGTTTGGATGTGCTGCCGTCCCAGTTTCCAACTTGCTCTGACCTCAACACCACCGCCTTTGTCCTCCGCATGATCGAGGCGGAGAAGCCGAATCTCATCGTCTTCACCGGAGATAACATATTCGGGTTTGATGCCACGAATGGTGCTAAATCTTTGAACGAGGCATACGCCCCAGCAATCGCGTCCAACATCCCGTGGGCAGCTGTGCTGGGGAACCATGATCAGGAATCCGACCTCTCGAGGGAAGGGGTAATGAAACACATAGTTGGGTTGAAGAACACTCTGGCTCAAGTTAATCCTTTAGACAAAGATGTTATTGATGGTTTTGGGAACTATAACCTGGAGGTCGCCGGAGTTGAGGGCTCTGGTTTTGAAAACAAATCGGTTCTCAATCTTTACTTCCTTGACAGCGGAGATTACTCCACGGTTCCCTCCATCGGTGGTTATGGTTGGATCAAACCCTCCCAACAATATTGGTTTGAACAAACTTCTGCAAAGCTTCAGAAAGCATACATAAGCAAGCCACTGCCCCAGAAAGCACCTGCGCCAGGGCTCGCATACTTTCACATACCATTGCCGGAATTTGCTAGTTTTGACTCATCAAACTTTACCGGAGTGAGGCAAGAAGACATCAGCTCTGCTTCTGTGAACTCGGGCTTCTTCACAACCATGGTTGCAGCTGGAGATGTCAAGGCAGTGTTCACAGGTCACGATCACCTCAATGACTTCTGCGGGGAGCTAACTGGCATAAACCTTTGTTATGCTGGGGGATTTGGATACCATGCTTATGGGAAGGCTGGATGGGATCGGAGAGCAAGGGTTGTGGTAGCAAACTTAGAGAAAACGCAAAAGGGAGGATGGGGAGCGGTCAAGTCAATCAAAACATGGAAGCGCCTTGATGACGAGCACCTCACTGCTATTGATGGTCAGGCTCTTTGGAGCAAGAGCTCTGTAGGTATTCGTAGAAAACCTGTTGGCGGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

406

Amino Acids

44.02

Weight (kDa)

6.6

Isoelectric Point (pI)

38.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 60 - 316 1.1e-12 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 781
Acc36I ACCTGC 1 cut(s) 781
AccBSI CCGCTC 1 cut(s) 1097
AccII CGCG 1 cut(s) 413
AclWI GGATC 2 cut(s) 692, 1044
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 1 cut(s) 812
AcuI CTGAAG 1 cut(s) 716
AfaI GTAC 1 cut(s) 10
AfiI CCNNNNNNNGG 7 cut(s) 95, 454, 472, 668, 779, 849, 1208
AgsI TTSAA 4 cut(s) 388, 505, 605, 713
AjnI CCWGG 2 cut(s) 570, 778
AluBI AGCT 7 cut(s) 154, 434, 730, 867, 911, 970, 1179
AluI AGCT 7 cut(s) 154, 434, 730, 867, 911, 970, 1179
Alw21I GWGCWC 2 cut(s) 1140, 1181
AlwI GGATC 2 cut(s) 692, 1044
Ama87I CYCGRG 2 cut(s) 469, 883
AoxI GGCC 1 cut(s) 102
ApeKI GCWGC 5 cut(s) 82, 154, 244, 431, 908
ApoI RAATTY 1 cut(s) 812
AspLEI GCGC 3 cut(s) 82, 778, 1125
AsuHPI GGTGA 2 cut(s) 328, 938
AvaI CYCGRG 2 cut(s) 469, 883
BanII GRGCYC 3 cut(s) 596, 786, 1181
BbsI GAAGAC 2 cut(s) 325, 864
Bbv12I GWGCWC 2 cut(s) 1140, 1181
BbvI GCAGC 5 cut(s) 69, 141, 231, 443, 920
BccI CCATC 7 cut(s) 164, 209, 545, 674, 1026, 1083, 1148
BceAI ACGGC 1 cut(s) 232
BciT130I CCWGG 2 cut(s) 572, 780
BciVI GTATCC 1 cut(s) 1001
BclI TGATCA 1 cut(s) 451
BfaI CTAG 1 cut(s) 819
BfmI CTRYAG 1 cut(s) 1182
BfoI RGCGCY 2 cut(s) 83, 1126
BfuAI ACCTGC 1 cut(s) 781
BfuI GTATCC 1 cut(s) 1001
BisI GCNGC 8 cut(s) 69, 83, 105, 108, 155, 245, 432, 909
BlsI GCNGC 8 cut(s) 70, 84, 106, 109, 156, 246, 433, 910
Bme1390I CCNGG 2 cut(s) 572, 780
BmeT110I CYCGRG 2 cut(s) 469, 883
BmiI GGNNCC 2 cut(s) 446, 660
BmrFI CCNGG 2 cut(s) 572, 780
BmrI ACTGGG 1 cut(s) 247
BmsI GCATC 1 cut(s) 352
BmuI ACTGGG 1 cut(s) 247
BpiI GAAGAC 2 cut(s) 325, 864
BpmI CTGGAG 2 cut(s) 593, 933
Bpu10I CCTNAGC 1 cut(s) 47
BpuEI CTTGAG 1 cut(s) 504
BsaJI CCNNGG 5 cut(s) 130, 425, 654, 779, 900
BsaWI WCCGGW 2 cut(s) 338, 842
Bsc4I CCNNNNNNNGG 7 cut(s) 95, 454, 472, 668, 779, 849, 1208
Bse1I ACTGG 2 cut(s) 253, 979
Bse3DI GCAATG 1 cut(s) 803
BseBI CCWGG 2 cut(s) 572, 780
BseDI CCNNGG 5 cut(s) 130, 425, 654, 779, 900
BseGI GGATG 5 cut(s) 30, 244, 420, 1037, 1094
BseLI CCNNNNNNNGG 7 cut(s) 95, 454, 472, 668, 779, 849, 1208
BseMI GCAATG 1 cut(s) 803
BseNI ACTGG 2 cut(s) 253, 979
BseXI GCAGC 5 cut(s) 69, 141, 231, 443, 920
BseYI CCCAGC 3 cut(s) 403, 439, 995
Bsh1236I CGCG 1 cut(s) 413
BshFI GGCC 1 cut(s) 104
BsiHKAI GWGCWC 2 cut(s) 1140, 1181
BsiHKCI CYCGRG 2 cut(s) 469, 883
BsiSI CCGG 4 cut(s) 339, 582, 809, 843
BslFI GGGAC 3 cut(s) 47, 178, 235
BslI CCNNNNNNNGG 7 cut(s) 95, 454, 472, 668, 779, 849, 1208
BsmFI GGGAC 3 cut(s) 47, 178, 235
BsnI GGCC 1 cut(s) 104
BsoBI CYCGRG 2 cut(s) 469, 883
Bsp1286I GDGCHC 4 cut(s) 596, 786, 1140, 1181
Bsp143I GATC 5 cut(s) 303, 451, 684, 943, 1036
Bsp19I CCATGG 1 cut(s) 900
BspANI GGCC 1 cut(s) 104
BspFNI CGCG 1 cut(s) 413
BspLI GGNNCC 2 cut(s) 446, 660
BspMI ACCTGC 1 cut(s) 781
BspPI GGATC 2 cut(s) 692, 1044
BsrBI CCGCTC 1 cut(s) 1097
BsrDI GCAATG 1 cut(s) 803
BsrI ACTGG 2 cut(s) 253, 979
BssECI CCNNGG 5 cut(s) 130, 425, 654, 779, 900
BssMI GATC 5 cut(s) 303, 451, 684, 943, 1036
BssT1I CCWWGG 2 cut(s) 130, 900
Bst2UI CCWGG 2 cut(s) 572, 780
Bst4CI ACNGT 3 cut(s) 13, 19, 658
Bst6I CTCTTC 1 cut(s) 23
BstC8I GCNNGC 2 cut(s) 752, 786
BstDEI CTNAG 2 cut(s) 47, 1066
BstDSI CCRYGG 3 cut(s) 425, 654, 900
BstENI CCTNNNNNAGG 1 cut(s) 777
BstF5I GGATG 5 cut(s) 30, 244, 420, 1037, 1094
BstFNI CGCG 1 cut(s) 413
BstH2I RGCGCY 2 cut(s) 83, 1126
BstHHI GCGC 3 cut(s) 82, 778, 1125
BstKTI GATC 5 cut(s) 306, 454, 687, 946, 1039
BstMBI GATC 5 cut(s) 303, 451, 684, 943, 1036
BstMWI GCNNNNNNNGC 1 cut(s) 77
BstNI CCWGG 2 cut(s) 572, 780
BstSCI CCNGG 2 cut(s) 570, 778
BstSFI CTRYAG 1 cut(s) 1182
BstUI CGCG 1 cut(s) 413
BstV1I GCAGC 5 cut(s) 69, 141, 231, 443, 920
BstV2I GAAGAC 2 cut(s) 325, 864
BstXI CCANNNNNNTGG 1 cut(s) 1019
BsuI GTATCC 1 cut(s) 1001
BsuRI GGCC 1 cut(s) 104
BtgI CCRYGG 3 cut(s) 425, 654, 900
BtsCI GGATG 5 cut(s) 30, 244, 420, 1037, 1094
BtsI GCAGTG 3 cut(s) 755, 933, 1143
BtsIMutI CAGTG 5 cut(s) 18, 24, 755, 933, 1143
BveI ACCTGC 1 cut(s) 781
Cac8I GCNNGC 2 cut(s) 752, 786
CfoI GCGC 3 cut(s) 82, 778, 1125
CseI GACGC 1 cut(s) 402
Csp6I GTAC 1 cut(s) 9
CspCI CAANNNNNGTGG 4 cut(s) 271, 306, 1035, 1070
CviAII CATG 6 cut(s) 37, 301, 449, 901, 1013, 1116
CviQI GTAC 1 cut(s) 9
DdeI CTNAG 2 cut(s) 47, 1066
DpnI GATC 5 cut(s) 305, 453, 686, 945, 1038
DpnII GATC 5 cut(s) 303, 451, 684, 943, 1036
EaeI YGGCCR 1 cut(s) 102
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
EciI GGCGGA 2 cut(s) 212, 326
Ecl136II GAGCTC 1 cut(s) 1179
Eco130I CCWWGG 2 cut(s) 130, 900
Eco24I GRGCYC 3 cut(s) 596, 786, 1181
Eco53kI GAGCTC 1 cut(s) 1179
Eco57I CTGAAG 1 cut(s) 716
Eco88I CYCGRG 2 cut(s) 469, 883
EcoICRI GAGCTC 1 cut(s) 1179
EcoNI CCTNNNNNAGG 1 cut(s) 777
EcoRII CCWGG 2 cut(s) 570, 778
EcoT14I CCWWGG 2 cut(s) 130, 900
EcoT38I GRGCYC 3 cut(s) 596, 786, 1181
ErhI CCWWGG 2 cut(s) 130, 900
FaeI CATG 6 cut(s) 40, 304, 452, 904, 1016, 1119
FaqI GGGAC 3 cut(s) 47, 178, 235
FatI CATG 6 cut(s) 36, 300, 448, 900, 1012, 1115
FauI CCCGC 1 cut(s) 956
FbaI TGATCA 1 cut(s) 451
Fnu4HI GCNGC 8 cut(s) 69, 83, 105, 108, 155, 245, 432, 909
FokI GGATG 5 cut(s) 37, 251, 407, 1044, 1101
FriOI GRGCYC 3 cut(s) 596, 786, 1181
Fsp4HI GCNGC 8 cut(s) 69, 83, 105, 108, 155, 245, 432, 909
FspBI CTAG 1 cut(s) 819
GlaI GCGC 3 cut(s) 81, 777, 1124
GluI GCNGC 8 cut(s) 69, 83, 105, 108, 155, 245, 432, 909
GsaI CCCAGC 3 cut(s) 407, 443, 999
GsuI CTGGAG 2 cut(s) 593, 933
HaeII RGCGCY 2 cut(s) 83, 1126
HaeIII GGCC 1 cut(s) 104
HapII CCGG 4 cut(s) 339, 582, 809, 843
HgaI GACGC 1 cut(s) 402
HhaI GCGC 3 cut(s) 82, 778, 1125
Hin1II CATG 6 cut(s) 40, 304, 452, 904, 1016, 1119
Hin6I GCGC 3 cut(s) 80, 776, 1123
HinP1I GCGC 3 cut(s) 80, 776, 1123
HindIII AAGCTT 1 cut(s) 728
HinfI GANTC 5 cut(s) 52, 184, 322, 458, 827
HpaII CCGG 4 cut(s) 339, 582, 809, 843
HphI GGTGA 2 cut(s) 328, 938
Hpy166II GTNNAC 2 cut(s) 880, 933
Hpy188I TCNGA 4 cut(s) 271, 463, 735, 1041
Hpy188III TCNNGA 3 cut(s) 455, 469, 941
Hpy8I GTNNAC 2 cut(s) 880, 933
HpyAV CCTTC 2 cut(s) 470, 1018
HpyCH4III ACNGT 3 cut(s) 13, 19, 658
HpyCH4V TGCA 4 cut(s) 190, 205, 725, 908
HpyF10VI GCNNNNNNNGC 1 cut(s) 77
HpyF3I CTNAG 2 cut(s) 47, 1066
Hsp92II CATG 6 cut(s) 40, 304, 452, 904, 1016, 1119
HspAI GCGC 3 cut(s) 80, 776, 1123
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 5 cut(s) 303, 451, 684, 943, 1036
LmnI GCTCC 3 cut(s) 967, 1094, 1170
Lsp1109I GCAGC 5 cut(s) 69, 141, 231, 443, 920
LweI GCATC 1 cut(s) 352
MaeI CTAG 1 cut(s) 819
MaeIII GTNAC 2 cut(s) 13, 938
MalI GATC 5 cut(s) 305, 453, 686, 945, 1038
MbiI CCGCTC 1 cut(s) 1097
MboI GATC 5 cut(s) 303, 451, 684, 943, 1036
MboII GAAGA 6 cut(s) 40, 160, 325, 517, 869, 883
MhlI GDGCHC 4 cut(s) 596, 786, 1140, 1181
MluCI AATT 2 cut(s) 812, 1213
MlyI GAGTC 2 cut(s) 61, 821
MmeI TCCRAC 4 cut(s) 284, 441, 486, 662
MseI TTAA 2 cut(s) 180, 525
MslI CAYNNNNRTG 4 cut(s) 41, 899, 951, 1017
MspA1I CMGCKG 3 cut(s) 434, 642, 911
MspI CCGG 4 cut(s) 339, 582, 809, 843
MspR9I CCNGG 2 cut(s) 572, 780
MvaI CCWGG 2 cut(s) 572, 780
MvnI CGCG 1 cut(s) 413
MwoI GCNNNNNNNGC 1 cut(s) 77
NcoI CCATGG 1 cut(s) 900
NdeII GATC 5 cut(s) 303, 451, 684, 943, 1036
NlaIII CATG 6 cut(s) 40, 304, 452, 904, 1016, 1119
NlaIV GGNNCC 2 cut(s) 446, 660
NmuCI GTSAC 2 cut(s) 13, 938
PaeR7I CTCGAG 1 cut(s) 469
PaqCI CACCTGC 1 cut(s) 781
PfeI GAWTC 3 cut(s) 184, 322, 458
PkrI GCNGC 8 cut(s) 70, 84, 106, 109, 156, 246, 433, 910
PleI GAGTC 2 cut(s) 60, 821
PpsI GAGTC 2 cut(s) 60, 821
Psp124BI GAGCTC 1 cut(s) 1181
Psp6I CCWGG 2 cut(s) 570, 778
PspFI CCCAGC 3 cut(s) 403, 439, 995
PspGI CCWGG 2 cut(s) 570, 778
PspN4I GGNNCC 2 cut(s) 446, 660
PsrI GAACNNNNNNTAC 2 cut(s) 380, 412
PvuII CAGCTG 2 cut(s) 434, 911
RsaI GTAC 1 cut(s) 10
RsaNI GTAC 1 cut(s) 9
RseI CAYNNNNRTG 4 cut(s) 41, 899, 951, 1017
SacI GAGCTC 1 cut(s) 1181
SaqAI TTAA 2 cut(s) 180, 525
SatI GCNGC 8 cut(s) 69, 83, 105, 108, 155, 245, 432, 909
Sau3AI GATC 5 cut(s) 303, 451, 684, 943, 1036
SchI GAGTC 2 cut(s) 61, 821
ScrFI CCNGG 2 cut(s) 572, 780
SduI GDGCHC 4 cut(s) 596, 786, 1140, 1181
SfaNI GCATC 1 cut(s) 352
SfcI CTRYAG 1 cut(s) 1182
Sfr274I CTCGAG 1 cut(s) 469
SlaI CTCGAG 1 cut(s) 469
SmiMI CAYNNNNRTG 4 cut(s) 41, 899, 951, 1017
SmlI CTYRAG 2 cut(s) 469, 519
SmoI CTYRAG 2 cut(s) 469, 519
Sse9I AATT 2 cut(s) 812, 1213
SspI AATATT 1 cut(s) 704
SspMI CTAG 1 cut(s) 819
SstI GAGCTC 1 cut(s) 1181
StyD4I CCNGG 2 cut(s) 570, 778
StyI CCWWGG 2 cut(s) 130, 900
TaaI ACNGT 3 cut(s) 13, 19, 658
TaqI TCGA 2 cut(s) 306, 470
TasI AATT 2 cut(s) 812, 1213
TauI GCSGC 3 cut(s) 71, 107, 110
TfiI GAWTC 3 cut(s) 184, 322, 458
Tru1I TTAA 2 cut(s) 180, 525
Tru9I TTAA 2 cut(s) 180, 525
TscAI CASTG 5 cut(s) 18, 24, 762, 933, 1150
TseFI GTSAC 2 cut(s) 13, 938
TseI GCWGC 5 cut(s) 82, 154, 244, 431, 908
Tsp45I GTSAC 2 cut(s) 13, 938
TspDTI ATGAA 2 cut(s) 41, 500
TspRI CASTG 5 cut(s) 18, 24, 762, 933, 1150
XagI CCTNNNNNAGG 1 cut(s) 777
XapI RAATTY 1 cut(s) 812
XcmI CCANNNNNNNNNTGG 2 cut(s) 137, 232
XhoI CTCGAG 1 cut(s) 469
XspI CTAG 1 cut(s) 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.