pycom01g11980
NAC Family

inactive purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0007460
Physical Location & Seq
Reverse (-)
12945720 .. 12947095
1376 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g11980.1

Sequence Viewer

Length: 987 bp
ATGGAGTTCAGAGTCTCTGGTTTGGGAGATGAGAGTGGTGCAGTGACAGTGAGGATGAAGAGGGACAGGGAGGTGCTAAGGAGTCCATTTTTAGTGGCGGCGGTGGTGGCGCTGCTTCCTATATGGGGTATGGCCGCCGCAGAACAACAGCACCACCACCAAGGTAGAGGTGGGGAGAAGAAGCTGAGGTTTGGGACAGATGGGCAGTTTAAGATTCTGCAAGTGGCGGATATGCACTATGCCGATGGCAAGACCACACCCTGTTTGGATGTGCTGCTGTCCCAGTTTCCAACTTGCTCTGACCTCAACACCACCACCTTTGTCCTCCGCATGATAGAGGTGGAGAAGCCGAATCTCATCGTCTTCACCGGAGATAACATATTCGGGTTTGATGCCACGAATGGTGCTAAATCTTTGAACGAGGCATACACCCCAGCAATCGTGTCCAACATCCCGTGGGCAGCTGTGCTGGGGAACCATGATCAGGAATCCGACCTCTCGAGGGAAGGGGTAATGAAACACATAGTTGGGTTGAAGAACACTCTGGTTCAAGTTAATCCTTTAGACCAAGATGTTATTGATGGTTTTGGGAACTATAACCTAGAGGTCGCCGGAGTTGAGGGCTCTGGTTTTGAAAACAAATCGGTTCTCAATCTTTACTTCCTTGACAGCAGAGATTACTCCACGGTTCCCTCCATCGGTGGTTATGGTTGGATCAAACCCTCCCAACAATATTGGTTTGAACAAACTTCTGCAAAGCTTCAGAAAGTGTACATAAGCAAGCCACTGCCCCAGAAAGCACCTGCGCCGGGGCTCGCATACTTTCACATACCATTGCCGGAATTTTCTAGTTTTGACTCATCAAACTTTACCAGAGTGTGGCAAGAAGACATTAGCTCTGCTTCTGTGAACTCGGGCTTCTTCACAACCATGGTTGCAGCAGGAGATGTGAAGGCAGTGTTCACAGGTCATCGATCACCTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

329

Amino Acids

36.07

Weight (kDa)

5.63

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 70 - 324 3.6e-10 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 811
Acc36I ACCTGC 1 cut(s) 811
AccB7I CCANNNNNTGG 1 cut(s) 879
AciI CCGC 6 cut(s) 98, 101, 135, 138, 227, 328
AclWI GGATC 1 cut(s) 722
AcoI YGGCCR 1 cut(s) 132
AcsI RAATTY 1 cut(s) 842
AcuI CTGAAG 1 cut(s) 746
AfaI GTAC 1 cut(s) 773
AfiI CCNNNNNNNGG 6 cut(s) 125, 484, 502, 698, 809, 879
AgsI TTSAA 5 cut(s) 418, 535, 551, 635, 743
AluBI AGCT 4 cut(s) 184, 464, 760, 897
AluI AGCT 4 cut(s) 184, 464, 760, 897
Alw26I GTCTC 1 cut(s) 19
AlwI GGATC 1 cut(s) 722
Ama87I CYCGRG 2 cut(s) 499, 913
AoxI GGCC 1 cut(s) 132
ApeKI GCWGC 4 cut(s) 112, 274, 461, 938
ApoI RAATTY 1 cut(s) 842
AspLEI GCGC 2 cut(s) 112, 808
AsuC2I CCSGG 1 cut(s) 810
AsuHPI GGTGA 2 cut(s) 358, 969
AvaI CYCGRG 2 cut(s) 499, 913
BanII GRGCYC 2 cut(s) 626, 816
BbsI GAAGAC 2 cut(s) 355, 894
BbvCI CCTCAGC 1 cut(s) 185
BbvI GCAGC 4 cut(s) 99, 261, 473, 950
BccI CCATC 4 cut(s) 194, 239, 575, 704
BclI TGATCA 1 cut(s) 481
BcnI CCSGG 1 cut(s) 810
BcoDI GTCTC 1 cut(s) 19
BfaI CTAG 2 cut(s) 602, 849
BfoI RGCGCY 1 cut(s) 113
BfuAI ACCTGC 1 cut(s) 811
BisI GCNGC 7 cut(s) 99, 113, 135, 138, 275, 462, 939
BlsI GCNGC 7 cut(s) 100, 114, 136, 139, 276, 463, 940
Bme1390I CCNGG 1 cut(s) 810
BmeT110I CYCGRG 2 cut(s) 499, 913
BmiI GGNNCC 2 cut(s) 476, 690
BmrFI CCNGG 1 cut(s) 810
BmrI ACTGGG 1 cut(s) 277
BmsI GCATC 1 cut(s) 382
BmuI ACTGGG 1 cut(s) 277
BpiI GAAGAC 2 cut(s) 355, 894
Bpu10I CCTNAGC 2 cut(s) 77, 185
BpuMI CCSGG 1 cut(s) 810
Bsa29I ATCGAT 1 cut(s) 973
BsaJI CCNNGG 5 cut(s) 160, 455, 684, 809, 930
BsaWI WCCGGW 1 cut(s) 368
Bsc4I CCNNNNNNNGG 6 cut(s) 125, 484, 502, 698, 809, 879
Bse1I ACTGG 1 cut(s) 283
Bse3DI GCAATG 1 cut(s) 833
BseCI ATCGAT 1 cut(s) 973
BseDI CCNNGG 5 cut(s) 160, 455, 684, 809, 930
BseGI GGATG 3 cut(s) 60, 274, 450
BseLI CCNNNNNNNGG 6 cut(s) 125, 484, 502, 698, 809, 879
BseMI GCAATG 1 cut(s) 833
BseMII CTCAG 1 cut(s) 176
BseNI ACTGG 1 cut(s) 283
BseXI GCAGC 4 cut(s) 99, 261, 473, 950
BseYI CCCAGC 2 cut(s) 433, 469
BsgI GTGCAG 1 cut(s) 60
BshFI GGCC 1 cut(s) 134
BshVI ATCGAT 1 cut(s) 973
BsiHKCI CYCGRG 2 cut(s) 499, 913
BsiSI CCGG 4 cut(s) 369, 612, 809, 839
BslFI GGGAC 3 cut(s) 77, 208, 265
BslI CCNNNNNNNGG 6 cut(s) 125, 484, 502, 698, 809, 879
BsmAI GTCTC 1 cut(s) 19
BsmFI GGGAC 3 cut(s) 77, 208, 265
BsnI GGCC 1 cut(s) 134
BsoBI CYCGRG 2 cut(s) 499, 913
Bsp1286I GDGCHC 2 cut(s) 626, 816
Bsp1407I TGTACA 1 cut(s) 771
Bsp143I GATC 3 cut(s) 481, 714, 974
Bsp19I CCATGG 1 cut(s) 930
BspACI CCGC 6 cut(s) 98, 101, 135, 138, 227, 328
BspANI GGCC 1 cut(s) 134
BspCNI CTCAG 1 cut(s) 177
BspDI ATCGAT 1 cut(s) 973
BspLI GGNNCC 2 cut(s) 476, 690
BspMI ACCTGC 1 cut(s) 811
BspPI GGATC 1 cut(s) 722
BsrDI GCAATG 1 cut(s) 833
BsrGI TGTACA 1 cut(s) 771
BsrI ACTGG 1 cut(s) 283
BssECI CCNNGG 5 cut(s) 160, 455, 684, 809, 930
BssMI GATC 3 cut(s) 481, 714, 974
BssT1I CCWWGG 2 cut(s) 160, 930
Bst4CI ACNGT 2 cut(s) 49, 688
Bst6I CTCTTC 1 cut(s) 53
BstAUI TGTACA 1 cut(s) 771
BstC8I GCNNGC 2 cut(s) 782, 816
BstDEI CTNAG 2 cut(s) 77, 185
BstDSI CCRYGG 3 cut(s) 455, 684, 930
BstF5I GGATG 3 cut(s) 60, 274, 450
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 2 cut(s) 112, 808
BstKTI GATC 3 cut(s) 484, 717, 977
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 3 cut(s) 481, 714, 974
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 808
BstV1I GCAGC 4 cut(s) 99, 261, 473, 950
BstV2I GAAGAC 2 cut(s) 355, 894
Bsu15I ATCGAT 1 cut(s) 973
BsuRI GGCC 1 cut(s) 134
BsuTUI ATCGAT 1 cut(s) 973
BtgI CCRYGG 3 cut(s) 455, 684, 930
BtsCI GGATG 3 cut(s) 60, 274, 450
BtsI GCAGTG 3 cut(s) 48, 785, 963
BtsIMutI CAGTG 4 cut(s) 48, 54, 785, 963
BveI ACCTGC 1 cut(s) 811
Cac8I GCNNGC 2 cut(s) 782, 816
CfoI GCGC 2 cut(s) 112, 808
ClaI ATCGAT 1 cut(s) 973
Csp6I GTAC 1 cut(s) 772
CspCI CAANNNNNGTGG 2 cut(s) 301, 336
CviAII CATG 3 cut(s) 331, 479, 931
CviQI GTAC 1 cut(s) 772
DdeI CTNAG 2 cut(s) 77, 185
DpnI GATC 3 cut(s) 483, 716, 976
DpnII GATC 3 cut(s) 481, 714, 974
EaeI YGGCCR 1 cut(s) 132
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
EciI GGCGGA 1 cut(s) 242
Eco130I CCWWGG 2 cut(s) 160, 930
Eco24I GRGCYC 2 cut(s) 626, 816
Eco57I CTGAAG 1 cut(s) 746
Eco88I CYCGRG 2 cut(s) 499, 913
EcoT14I CCWWGG 2 cut(s) 160, 930
EcoT38I GRGCYC 2 cut(s) 626, 816
ErhI CCWWGG 2 cut(s) 160, 930
FaeI CATG 3 cut(s) 334, 482, 934
FaqI GGGAC 3 cut(s) 77, 208, 265
FatI CATG 3 cut(s) 330, 478, 930
FbaI TGATCA 1 cut(s) 481
Fnu4HI GCNGC 7 cut(s) 99, 113, 135, 138, 275, 462, 939
FokI GGATG 3 cut(s) 67, 281, 437
FriOI GRGCYC 2 cut(s) 626, 816
Fsp4HI GCNGC 7 cut(s) 99, 113, 135, 138, 275, 462, 939
FspBI CTAG 2 cut(s) 602, 849
GlaI GCGC 2 cut(s) 111, 807
GluI GCNGC 7 cut(s) 99, 113, 135, 138, 275, 462, 939
GsaI CCCAGC 2 cut(s) 437, 473
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 1 cut(s) 134
HapII CCGG 4 cut(s) 369, 612, 809, 839
HhaI GCGC 2 cut(s) 112, 808
Hin1II CATG 3 cut(s) 334, 482, 934
Hin6I GCGC 2 cut(s) 110, 806
HinP1I GCGC 2 cut(s) 110, 806
HindIII AAGCTT 1 cut(s) 758
HinfI GANTC 6 cut(s) 12, 82, 214, 352, 488, 857
HpaII CCGG 4 cut(s) 369, 612, 809, 839
HphI GGTGA 2 cut(s) 358, 969
Hpy166II GTNNAC 3 cut(s) 772, 910, 963
Hpy188I TCNGA 4 cut(s) 11, 301, 493, 765
Hpy188III TCNNGA 2 cut(s) 485, 499
Hpy8I GTNNAC 3 cut(s) 772, 910, 963
HpyAV CCTTC 2 cut(s) 500, 946
HpyCH4III ACNGT 2 cut(s) 49, 688
HpyCH4V TGCA 5 cut(s) 41, 220, 235, 755, 938
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 2 cut(s) 77, 185
Hsp92II CATG 3 cut(s) 334, 482, 934
HspAI GCGC 2 cut(s) 110, 806
Ksp22I TGATCA 1 cut(s) 481
Kzo9I GATC 3 cut(s) 481, 714, 974
Lsp1109I GCAGC 4 cut(s) 99, 261, 473, 950
LweI GCATC 1 cut(s) 382
MaeI CTAG 2 cut(s) 602, 849
MaeIII GTNAC 1 cut(s) 43
MalI GATC 3 cut(s) 483, 716, 976
MboI GATC 3 cut(s) 481, 714, 974
MboII GAAGA 6 cut(s) 70, 190, 355, 547, 899, 913
MhlI GDGCHC 2 cut(s) 626, 816
MluCI AATT 1 cut(s) 842
MlyI GAGTC 3 cut(s) 21, 91, 851
MmeI TCCRAC 4 cut(s) 314, 471, 516, 692
MseI TTAA 2 cut(s) 210, 555
MslI CAYNNNNRTG 2 cut(s) 929, 982
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 4 cut(s) 369, 612, 809, 839
MspR9I CCNGG 1 cut(s) 810
MwoI GCNNNNNNNGC 1 cut(s) 107
NciI CCSGG 1 cut(s) 810
NcoI CCATGG 1 cut(s) 930
NdeII GATC 3 cut(s) 481, 714, 974
NlaIII CATG 3 cut(s) 334, 482, 934
NlaIV GGNNCC 2 cut(s) 476, 690
NmuCI GTSAC 1 cut(s) 43
PaeR7I CTCGAG 1 cut(s) 499
PaqCI CACCTGC 1 cut(s) 811
PfeI GAWTC 3 cut(s) 214, 352, 488
PflMI CCANNNNNTGG 1 cut(s) 879
PkrI GCNGC 7 cut(s) 100, 114, 136, 139, 276, 463, 940
PleI GAGTC 3 cut(s) 20, 90, 851
PpsI GAGTC 3 cut(s) 20, 90, 851
PspFI CCCAGC 2 cut(s) 433, 469
PspN4I GGNNCC 2 cut(s) 476, 690
PsrI GAACNNNNNNTAC 2 cut(s) 410, 442
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 1 cut(s) 773
RsaNI GTAC 1 cut(s) 772
RseI CAYNNNNRTG 2 cut(s) 929, 982
SaqAI TTAA 2 cut(s) 210, 555
SatI GCNGC 7 cut(s) 99, 113, 135, 138, 275, 462, 939
Sau3AI GATC 3 cut(s) 481, 714, 974
SchI GAGTC 3 cut(s) 21, 91, 851
ScrFI CCNGG 1 cut(s) 810
SduI GDGCHC 2 cut(s) 626, 816
SfaNI GCATC 1 cut(s) 382
Sfr274I CTCGAG 1 cut(s) 499
SlaI CTCGAG 1 cut(s) 499
SmiMI CAYNNNNRTG 2 cut(s) 929, 982
SmlI CTYRAG 1 cut(s) 499
SmoI CTYRAG 1 cut(s) 499
Sse9I AATT 1 cut(s) 842
SsiI CCGC 6 cut(s) 98, 101, 135, 138, 227, 328
SspI AATATT 1 cut(s) 734
SspMI CTAG 2 cut(s) 602, 849
StyD4I CCNGG 1 cut(s) 808
StyI CCWWGG 2 cut(s) 160, 930
TaaI ACNGT 2 cut(s) 49, 688
TaqI TCGA 2 cut(s) 500, 973
TasI AATT 1 cut(s) 842
TatI WGTACW 1 cut(s) 771
TauI GCSGC 3 cut(s) 101, 137, 140
TfiI GAWTC 3 cut(s) 214, 352, 488
Tru1I TTAA 2 cut(s) 210, 555
Tru9I TTAA 2 cut(s) 210, 555
TscAI CASTG 4 cut(s) 48, 54, 792, 963
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 4 cut(s) 112, 274, 461, 938
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 2 cut(s) 71, 530
TspRI CASTG 4 cut(s) 48, 54, 792, 963
Van91I CCANNNNNTGG 1 cut(s) 879
XapI RAATTY 1 cut(s) 842
XcmI CCANNNNNNNNNTGG 2 cut(s) 167, 262
XhoI CTCGAG 1 cut(s) 499
XspI CTAG 2 cut(s) 602, 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.