pycom01g12530
NAC Family

inactive purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0007460
Physical Location & Seq
Reverse (-)
13275133 .. 13277506
2374 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g12530.1

Sequence Viewer

Length: 1098 bp
ATGGTGGTTCAGAGTCTCTGTTGTGGAGATGAGAGTGGTGCAGTGACAGTGAGGATGAAGAGGGACAGGGAGGTGCTAAGGAGTCTATTTTTAGCGGCGGTGGTGGTGATGGCGCTGCTTCCGATATGGGGTATGGCGGCCGCAGAACAACAGCACCACCAACGAGGTGGAGGTGGGGAGAAGAAGCTGAGGTTTAGGACAGATGGGCAGTTTAAGATTCTGCAAGTGGCGGATATGCACTATGCCGATGGCAAGACCACGCGCTGTTTGGATGTGCTGCCGTCCCAGTTTCCAACTTGCTCTGACCTCAACACCACCGCCTTTGTCCTCCGCATGATCGAGGCGGAGAAGCCGAATCTCATCGTCTTCACCGGAGATAACATATTCGGGTTTGATGCCACAAATGGTGCTAAATCTTTGATCGAGGCATACGGCCCAGCAATCGCGTGCAACATCCCGTGGGCAGCTGTGTTGGGGAACCATGATCGATCTTCTAGGAATCCGACCTCTCGAGGGAAGGGCGGAGATTACTCCACGGTTCCCTCCATCGGTGGTTATGGTTGGATCAAACCCTCCCAACAATATTGGTTTGAACAAACTTCTTCAAAGCTTCAGAAAGCGTACATAAGCAAGCCACTGCCCCAGAAGGCACCTGCGCCAGGGCTTGCATACTTTCACATACCATTGCCGGAATTTGCCAGTTTTGACTCATCAAACTTTACCGGAGTGAGGCAAGAAGACATTAGCTCTGCTTCTGTGAACTCGGGCTTCTTCACAACCATGGTTGCAGCAGGAGATGTGAAGGCTGTGTTCACAGGTCACGATCACCTCAATGACTTCTGCGGGGAGCTAACTGGCATAAACCTTTGTTATGCTGGGGGATTTGGATACCATGCTTATGGGAAGGCTGGATGGGATCGGAGAGCAAGGGTGGTGGTAGCAAACTTAGAGAAAACGAAAAAGGGAGGTTGGGGAGCGGTCAAGTCAATCAAAACATGGAAGCGCCTTGATGATGAGCACCTCACTGCTTTTGATGGTCAGGTTCTCTGGAGCAAGAGCTCTGTAGGTATTCGTAGAAAACCTGTTGGCGGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

366

Amino Acids

39.79

Weight (kDa)

9.13

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 71 - 276 4.3e-11 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 661
Acc36I ACCTGC 1 cut(s) 661
AccB1I GGYRCC 1 cut(s) 649
AccBSI CCGCTC 1 cut(s) 977
AccII CGCG 2 cut(s) 262, 446
AclWI GGATC 2 cut(s) 572, 924
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 1 cut(s) 692
AcuI CTGAAG 1 cut(s) 596
AfaI GTAC 1 cut(s) 623
AfiI CCNNNNNNNGG 6 cut(s) 128, 513, 548, 659, 729, 1088
AgsI TTSAA 2 cut(s) 593, 606
AjnI CCWGG 1 cut(s) 658
AluBI AGCT 6 cut(s) 187, 467, 610, 747, 850, 1059
AluI AGCT 6 cut(s) 187, 467, 610, 747, 850, 1059
Alw21I GWGCWC 2 cut(s) 1020, 1061
Alw26I GTCTC 1 cut(s) 20
AlwI GGATC 2 cut(s) 572, 924
Ama87I CYCGRG 2 cut(s) 510, 763
AoxI GGCC 2 cut(s) 138, 433
ApeKI GCWGC 4 cut(s) 115, 277, 464, 788
ApoI RAATTY 1 cut(s) 692
AspLEI GCGC 4 cut(s) 115, 264, 658, 1005
AspS9I GGNCC 1 cut(s) 434
AsuHPI GGTGA 3 cut(s) 118, 361, 818
AvaI CYCGRG 2 cut(s) 510, 763
BanI GGYRCC 1 cut(s) 649
BanII GRGCYC 1 cut(s) 1061
BbsI GAAGAC 2 cut(s) 358, 744
Bbv12I GWGCWC 2 cut(s) 1020, 1061
BbvCI CCTCAGC 1 cut(s) 188
BbvI GCAGC 4 cut(s) 102, 264, 476, 800
BccI CCATC 6 cut(s) 103, 197, 242, 554, 906, 1028
BceAI ACGGC 2 cut(s) 265, 448
BciT130I CCWGG 1 cut(s) 660
BciVI GTATCC 1 cut(s) 881
BcoDI GTCTC 1 cut(s) 20
BfaI CTAG 1 cut(s) 495
BfmI CTRYAG 1 cut(s) 1062
BfoI RGCGCY 2 cut(s) 116, 1006
BfuAI ACCTGC 1 cut(s) 661
BfuI GTATCC 1 cut(s) 881
BisI GCNGC 7 cut(s) 96, 116, 138, 141, 278, 465, 789
BlsI GCNGC 7 cut(s) 97, 117, 139, 142, 279, 466, 790
Bme1390I CCNGG 1 cut(s) 660
BmeT110I CYCGRG 2 cut(s) 510, 763
BmgT120I GGNCC 1 cut(s) 434
BmiI GGNNCC 3 cut(s) 479, 540, 651
BmrFI CCNGG 1 cut(s) 660
BmrI ACTGGG 1 cut(s) 280
BmsI GCATC 1 cut(s) 385
BmuI ACTGGG 1 cut(s) 280
BpiI GAAGAC 2 cut(s) 358, 744
BpmI CTGGAG 1 cut(s) 1069
Bpu10I CCTNAGC 2 cut(s) 77, 188
Bsa29I ATCGAT 1 cut(s) 487
BsaJI CCNNGG 4 cut(s) 458, 534, 659, 780
BsaWI WCCGGW 2 cut(s) 371, 722
Bsc4I CCNNNNNNNGG 6 cut(s) 128, 513, 548, 659, 729, 1088
Bse1I ACTGG 3 cut(s) 286, 699, 859
Bse3DI GCAATG 1 cut(s) 683
BseBI CCWGG 1 cut(s) 660
BseCI ATCGAT 1 cut(s) 487
BseDI CCNNGG 4 cut(s) 458, 534, 659, 780
BseGI GGATG 4 cut(s) 60, 277, 453, 917
BseLI CCNNNNNNNGG 6 cut(s) 128, 513, 548, 659, 729, 1088
BseMI GCAATG 1 cut(s) 683
BseMII CTCAG 1 cut(s) 179
BseNI ACTGG 3 cut(s) 286, 699, 859
BseX3I CGGCCG 1 cut(s) 138
BseXI GCAGC 4 cut(s) 102, 264, 476, 800
BseYI CCCAGC 2 cut(s) 436, 875
BsgI GTGCAG 1 cut(s) 60
Bsh1236I CGCG 2 cut(s) 262, 446
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 2 cut(s) 140, 435
BshNI GGYRCC 1 cut(s) 649
BshVI ATCGAT 1 cut(s) 487
BsiEI CGRYCG 1 cut(s) 141
BsiHKAI GWGCWC 2 cut(s) 1020, 1061
BsiHKCI CYCGRG 2 cut(s) 510, 763
BsiSI CCGG 3 cut(s) 372, 689, 723
BslFI GGGAC 2 cut(s) 77, 268
BslI CCNNNNNNNGG 6 cut(s) 128, 513, 548, 659, 729, 1088
BsmAI GTCTC 1 cut(s) 20
BsmFI GGGAC 2 cut(s) 77, 268
BsnI GGCC 2 cut(s) 140, 435
BsoBI CYCGRG 2 cut(s) 510, 763
Bsp1286I GDGCHC 2 cut(s) 1020, 1061
Bsp143I GATC 7 cut(s) 336, 420, 484, 488, 564, 823, 916
Bsp19I CCATGG 1 cut(s) 780
BspANI GGCC 2 cut(s) 140, 435
BspCNI CTCAG 1 cut(s) 180
BspDI ATCGAT 1 cut(s) 487
BspFNI CGCG 2 cut(s) 262, 446
BspLI GGNNCC 3 cut(s) 479, 540, 651
BspMI ACCTGC 1 cut(s) 661
BspPI GGATC 2 cut(s) 572, 924
BspT107I GGYRCC 1 cut(s) 649
BsrBI CCGCTC 1 cut(s) 977
BsrDI GCAATG 1 cut(s) 683
BsrI ACTGG 3 cut(s) 286, 699, 859
BssECI CCNNGG 4 cut(s) 458, 534, 659, 780
BssMI GATC 7 cut(s) 336, 420, 484, 488, 564, 823, 916
BssT1I CCWWGG 1 cut(s) 780
Bst2UI CCWGG 1 cut(s) 660
Bst4CI ACNGT 2 cut(s) 49, 538
Bst6I CTCTTC 1 cut(s) 53
BstC8I GCNNGC 3 cut(s) 448, 632, 666
BstDEI CTNAG 3 cut(s) 77, 188, 946
BstDSI CCRYGG 3 cut(s) 458, 534, 780
BstENI CCTNNNNNAGG 1 cut(s) 657
BstF5I GGATG 4 cut(s) 60, 277, 453, 917
BstFNI CGCG 2 cut(s) 262, 446
BstH2I RGCGCY 2 cut(s) 116, 1006
BstHHI GCGC 4 cut(s) 115, 264, 658, 1005
BstKTI GATC 7 cut(s) 339, 423, 487, 491, 567, 826, 919
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 7 cut(s) 336, 420, 484, 488, 564, 823, 916
BstMCI CGRYCG 1 cut(s) 141
BstNI CCWGG 1 cut(s) 660
BstSCI CCNGG 1 cut(s) 658
BstSFI CTRYAG 1 cut(s) 1062
BstUI CGCG 2 cut(s) 262, 446
BstV1I GCAGC 4 cut(s) 102, 264, 476, 800
BstV2I GAAGAC 2 cut(s) 358, 744
BstXI CCANNNNNNTGG 2 cut(s) 167, 899
BstZI CGGCCG 1 cut(s) 138
Bsu15I ATCGAT 1 cut(s) 487
BsuI GTATCC 1 cut(s) 881
BsuRI GGCC 2 cut(s) 140, 435
BsuTUI ATCGAT 1 cut(s) 487
BtgI CCRYGG 3 cut(s) 458, 534, 780
BtsCI GGATG 4 cut(s) 60, 277, 453, 917
BtsI GCAGTG 3 cut(s) 48, 635, 1023
BtsIMutI CAGTG 4 cut(s) 48, 54, 635, 1023
BveI ACCTGC 1 cut(s) 661
Cac8I GCNNGC 3 cut(s) 448, 632, 666
CciNI GCGGCCGC 1 cut(s) 138
CfoI GCGC 4 cut(s) 115, 264, 658, 1005
Cfr13I GGNCC 1 cut(s) 434
ClaI ATCGAT 1 cut(s) 487
Csp6I GTAC 1 cut(s) 622
CspCI CAANNNNNGTGG 4 cut(s) 304, 339, 915, 950
CviAII CATG 5 cut(s) 334, 482, 781, 893, 996
CviQI GTAC 1 cut(s) 622
DdeI CTNAG 3 cut(s) 77, 188, 946
DpnI GATC 7 cut(s) 338, 422, 486, 490, 566, 825, 918
DpnII GATC 7 cut(s) 336, 420, 484, 488, 564, 823, 916
EaeI YGGCCR 1 cut(s) 138
EagI CGGCCG 1 cut(s) 138
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
EciI GGCGGA 3 cut(s) 245, 359, 537
Ecl136II GAGCTC 1 cut(s) 1059
EclXI CGGCCG 1 cut(s) 138
Eco130I CCWWGG 1 cut(s) 780
Eco24I GRGCYC 1 cut(s) 1061
Eco52I CGGCCG 1 cut(s) 138
Eco53kI GAGCTC 1 cut(s) 1059
Eco57I CTGAAG 1 cut(s) 596
Eco88I CYCGRG 2 cut(s) 510, 763
EcoICRI GAGCTC 1 cut(s) 1059
EcoNI CCTNNNNNAGG 1 cut(s) 657
EcoRII CCWGG 1 cut(s) 658
EcoT14I CCWWGG 1 cut(s) 780
EcoT38I GRGCYC 1 cut(s) 1061
ErhI CCWWGG 1 cut(s) 780
FaeI CATG 5 cut(s) 337, 485, 784, 896, 999
FaqI GGGAC 2 cut(s) 77, 268
FatI CATG 5 cut(s) 333, 481, 780, 892, 995
FauI CCCGC 1 cut(s) 836
Fnu4HI GCNGC 7 cut(s) 96, 116, 138, 141, 278, 465, 789
FokI GGATG 4 cut(s) 67, 284, 440, 924
FriOI GRGCYC 1 cut(s) 1061
Fsp4HI GCNGC 7 cut(s) 96, 116, 138, 141, 278, 465, 789
FspBI CTAG 1 cut(s) 495
GlaI GCGC 4 cut(s) 114, 263, 657, 1004
GluI GCNGC 7 cut(s) 96, 116, 138, 141, 278, 465, 789
GsaI CCCAGC 2 cut(s) 440, 879
GsuI CTGGAG 1 cut(s) 1069
HaeII RGCGCY 2 cut(s) 116, 1006
HaeIII GGCC 2 cut(s) 140, 435
HapII CCGG 3 cut(s) 372, 689, 723
HhaI GCGC 4 cut(s) 115, 264, 658, 1005
Hin1II CATG 5 cut(s) 337, 485, 784, 896, 999
Hin6I GCGC 4 cut(s) 113, 262, 656, 1003
HinP1I GCGC 4 cut(s) 113, 262, 656, 1003
HindIII AAGCTT 1 cut(s) 608
HinfI GANTC 6 cut(s) 13, 82, 217, 355, 499, 707
HpaII CCGG 3 cut(s) 372, 689, 723
HphI GGTGA 3 cut(s) 118, 361, 818
Hpy166II GTNNAC 2 cut(s) 760, 813
Hpy188I TCNGA 6 cut(s) 12, 123, 304, 504, 615, 921
Hpy188III TCNNGA 3 cut(s) 510, 821, 1048
Hpy8I GTNNAC 2 cut(s) 760, 813
HpyAV CCTTC 4 cut(s) 511, 640, 796, 898
HpyCH4III ACNGT 2 cut(s) 49, 538
HpyCH4V TGCA 6 cut(s) 41, 223, 238, 450, 668, 788
HpyF3I CTNAG 3 cut(s) 77, 188, 946
Hsp92II CATG 5 cut(s) 337, 485, 784, 896, 999
HspAI GCGC 4 cut(s) 113, 262, 656, 1003
Kzo9I GATC 7 cut(s) 336, 420, 484, 488, 564, 823, 916
LmnI GCTCC 3 cut(s) 847, 974, 1050
Lsp1109I GCAGC 4 cut(s) 102, 264, 476, 800
LweI GCATC 1 cut(s) 385
MaeI CTAG 1 cut(s) 495
MaeIII GTNAC 2 cut(s) 43, 818
MalI GATC 7 cut(s) 338, 422, 486, 490, 566, 825, 918
MbiI CCGCTC 1 cut(s) 977
MboI GATC 7 cut(s) 336, 420, 484, 488, 564, 823, 916
MboII GAAGA 7 cut(s) 70, 193, 358, 483, 594, 749, 763
MhlI GDGCHC 2 cut(s) 1020, 1061
MluCI AATT 2 cut(s) 692, 1093
MlyI GAGTC 3 cut(s) 22, 91, 701
MmeI TCCRAC 3 cut(s) 317, 527, 542
MseI TTAA 1 cut(s) 213
MslI CAYNNNNRTG 3 cut(s) 779, 831, 897
MspA1I CMGCKG 1 cut(s) 467
MspI CCGG 3 cut(s) 372, 689, 723
MspR9I CCNGG 1 cut(s) 660
MvaI CCWGG 1 cut(s) 660
MvnI CGCG 2 cut(s) 262, 446
NcoI CCATGG 1 cut(s) 780
NdeII GATC 7 cut(s) 336, 420, 484, 488, 564, 823, 916
NlaIII CATG 5 cut(s) 337, 485, 784, 896, 999
NlaIV GGNNCC 3 cut(s) 479, 540, 651
NmuCI GTSAC 2 cut(s) 43, 818
NotI GCGGCCGC 1 cut(s) 138
PaeR7I CTCGAG 1 cut(s) 510
PaqCI CACCTGC 1 cut(s) 661
PfeI GAWTC 3 cut(s) 217, 355, 499
PkrI GCNGC 7 cut(s) 97, 117, 139, 142, 279, 466, 790
PleI GAGTC 3 cut(s) 21, 90, 701
PpsI GAGTC 3 cut(s) 21, 90, 701
Psp124BI GAGCTC 1 cut(s) 1061
Psp6I CCWGG 1 cut(s) 658
PspFI CCCAGC 2 cut(s) 436, 875
PspGI CCWGG 1 cut(s) 658
PspN4I GGNNCC 3 cut(s) 479, 540, 651
PspPI GGNCC 1 cut(s) 434
PvuII CAGCTG 1 cut(s) 467
RsaI GTAC 1 cut(s) 623
RsaNI GTAC 1 cut(s) 622
RseI CAYNNNNRTG 3 cut(s) 779, 831, 897
SacI GAGCTC 1 cut(s) 1061
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 7 cut(s) 96, 116, 138, 141, 278, 465, 789
Sau3AI GATC 7 cut(s) 336, 420, 484, 488, 564, 823, 916
Sau96I GGNCC 1 cut(s) 434
SchI GAGTC 3 cut(s) 22, 91, 701
ScrFI CCNGG 1 cut(s) 660
SduI GDGCHC 2 cut(s) 1020, 1061
SfaNI GCATC 1 cut(s) 385
SfcI CTRYAG 1 cut(s) 1062
Sfr274I CTCGAG 1 cut(s) 510
SlaI CTCGAG 1 cut(s) 510
SmiMI CAYNNNNRTG 3 cut(s) 779, 831, 897
SmlI CTYRAG 1 cut(s) 510
SmoI CTYRAG 1 cut(s) 510
Sse9I AATT 2 cut(s) 692, 1093
SspI AATATT 1 cut(s) 584
SspMI CTAG 1 cut(s) 495
SstI GAGCTC 1 cut(s) 1061
StyD4I CCNGG 1 cut(s) 658
StyI CCWWGG 1 cut(s) 780
TaaI ACNGT 2 cut(s) 49, 538
TaqI TCGA 4 cut(s) 339, 423, 487, 511
TasI AATT 2 cut(s) 692, 1093
TauI GCSGC 3 cut(s) 98, 140, 143
TfiI GAWTC 3 cut(s) 217, 355, 499
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 4 cut(s) 48, 54, 642, 1030
TseFI GTSAC 2 cut(s) 43, 818
TseI GCWGC 4 cut(s) 115, 277, 464, 788
Tsp45I GTSAC 2 cut(s) 43, 818
TspDTI ATGAA 1 cut(s) 71
TspRI CASTG 4 cut(s) 48, 54, 642, 1030
XagI CCTNNNNNAGG 1 cut(s) 657
XapI RAATTY 1 cut(s) 692
XcmI CCANNNNNNNNNTGG 2 cut(s) 164, 265
XhoI CTCGAG 1 cut(s) 510
XspI CTAG 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.