Rw1G026770
NAC Family

inactive purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0007460
Physical Location & Seq
Reverse (-)
54021134 .. 54024259
3126 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G026770.1

Sequence Viewer

Length: 1161 bp
ATGGGTCGTTTGTTCACGGCGGCGGCGTTGGTGGTGTTGCTTCCGATCTGGGTTTTGGCGGCGGCTAAGCACCATGAAGAGAAACCTATGAGGCTGAGGTTTGGGGGTGATGGGCAGTTTAAGATACTGCAAGTGGCGGACATGCACTATGCCGATGGGAAAACGACGCCGTGTGAGGATGTGCTGCCGTCACAGGTGGCGGGTTGCTCTGACCTTAATACGACGGCGTTTGTTCAGCGGATGATTCAGGCTGAGAAGCCCAATTTCATTGTTTTCACCGGAGATAACATCTTTGGGTCTGATGCTACGGATGCTGCTAAATCAATGGATGCAGCATTTGCCCCAGCAATTGCGTCCAACATTCCGTGGGCAGCTGTTTTGGGGAACCATGATCAGGAGTCGGACCTTTCGAGGGAAGGGGTAATGAAGCACATAGTCAAGTATAAAAGCACCCTGGCTCAACTCAATCCTTCGGATCAAGATGTTATTGATGGTTTCGGGAACTATAACCTGGAGGTAGGTGGAGTTCAGGGGACTGGTTTTGAAAACAAATCGGTTCTCAATCTATACTTCCTTGACAGTGGAGATTACTCCACAGTTCCCTCTATCCCTGGCTATGGTTGGATCAAACCCTCCCAGCAATTTTGGTTCCAACAAACTTCAGCAAAGCTAAAGAAAGCATATATGAAAAAGCCACAGGCGCAGAAATCACCGGCTCCAGGGCTCGCATACTTTCACATACCGTTACCAGAATATGCTAGTTTTGACTCATCAAACTTCACAGGAGTGAGGCAAGAAGGCATTAGCTCTGCCTCCGTGAACTCTGGCTTCTTCACAACCCTGGTTGAAGCGGGCGATGTAAAGGCAGCTTTCACAGGCCATGATCACCTCAATGACTTCTGTGGGAAGCTAACTGGCATAAACCTTTGTTATGCTGGGGGATTCGGATACCATGCATATGGGCAGGCAGGATGGGATAGGAGAGCAAGGGTGGTAGCAGCAACCTTAGAGAAAACAAATACTGGAGGTTGGGGAGCAGTCAAGTCAATCAAAACATGGAAACGCCTCGACGATCAGCACCTCAGTACTATCAATAGTGAGGTTCTCTGGACCAAGAGTTCTGCGGGTGCACGTAGAAAGAAACCCATTGGCGGTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

386

Amino Acids

41.89

Weight (kDa)

8.19

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 40 - 296 2.2e-13 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 483, 632
AcuI CTGAAG 1 cut(s) 645
AcyI GRCGYC 1 cut(s) 167
AfaI GTAC 1 cut(s) 1087
AfiI CCNNNNNNNGG 5 cut(s) 394, 412, 617, 719, 1151
AgsI TTSAA 2 cut(s) 545, 848
AjnI CCWGG 5 cut(s) 453, 510, 610, 718, 840
AleI CACNNNNGTG 1 cut(s) 785
AloI GAACNNNNNNTCC 2 cut(s) 1102, 1134
AluBI AGCT 5 cut(s) 374, 670, 807, 869, 910
AluI AGCT 5 cut(s) 374, 670, 807, 869, 910
Alw21I GWGCWC 1 cut(s) 1132
Alw44I GTGCAC 1 cut(s) 1128
AlwI GGATC 2 cut(s) 483, 632
AoxI GGCC 1 cut(s) 877
ApaLI GTGCAC 1 cut(s) 1128
ApeKI GCWGC 6 cut(s) 184, 314, 332, 371, 866, 998
AspLEI GCGC 1 cut(s) 703
AspS9I GGNCC 3 cut(s) 403, 1110, 1154
AsuHPI GGTGA 4 cut(s) 119, 268, 702, 878
AvaII GGWCC 3 cut(s) 403, 1110, 1154
BaeGI GKGCMC 1 cut(s) 1132
BanII GRGCYC 1 cut(s) 726
Bbv12I GWGCWC 1 cut(s) 1132
BbvCI CCTCAGC 1 cut(s) 95
BbvI GCAGC 6 cut(s) 171, 301, 344, 383, 878, 1010
BccI CCATC 4 cut(s) 104, 149, 485, 966
BceAI ACGGC 4 cut(s) 33, 154, 172, 240
BciT130I CCWGG 5 cut(s) 455, 512, 612, 720, 842
BciVI GTATCC 1 cut(s) 941
BclI TGATCA 2 cut(s) 391, 883
BfaI CTAG 1 cut(s) 759
BfuI GTATCC 1 cut(s) 941
BlpI GCTNAGC 1 cut(s) 66
BmcAI AGTACT 1 cut(s) 1087
Bme1390I CCNGG 5 cut(s) 455, 512, 612, 720, 842
Bme18I GGWCC 3 cut(s) 403, 1110, 1154
BmgT120I GGNCC 3 cut(s) 403, 1110, 1154
BmiI GGNNCC 3 cut(s) 386, 650, 717
BmrFI CCNGG 5 cut(s) 455, 512, 612, 720, 842
BmsI GCATC 3 cut(s) 292, 301, 319
BpmI CTGGAG 3 cut(s) 533, 702, 1044
Bpu10I CCTNAGC 1 cut(s) 95
Bpu1102I GCTNAGC 1 cut(s) 66
BsaAI YACGTR 1 cut(s) 1133
BsaHI GRCGYC 1 cut(s) 167
BsaJI CCNNGG 5 cut(s) 365, 453, 610, 719, 840
BsaWI WCCGGW 1 cut(s) 278
Bsc4I CCNNNNNNNGG 5 cut(s) 394, 412, 617, 719, 1151
Bse118I RCCGGY 1 cut(s) 712
Bse1I ACTGG 3 cut(s) 541, 919, 1027
BseBI CCWGG 5 cut(s) 455, 512, 612, 720, 842
BseDI CCNNGG 5 cut(s) 365, 453, 610, 719, 840
BseGI GGATG 5 cut(s) 184, 246, 316, 334, 977
BseLI CCNNNNNNNGG 5 cut(s) 394, 412, 617, 719, 1151
BseMII CTCAG 3 cut(s) 86, 243, 1096
BseNI ACTGG 3 cut(s) 541, 919, 1027
BseSI GKGCMC 1 cut(s) 1132
BseXI GCAGC 6 cut(s) 171, 301, 344, 383, 878, 1010
BseYI CCCAGC 3 cut(s) 343, 636, 935
BshFI GGCC 1 cut(s) 879
BsiHKAI GWGCWC 1 cut(s) 1132
BsiSI CCGG 2 cut(s) 279, 713
BslFI GGGAC 1 cut(s) 547
BslI CCNNNNNNNGG 5 cut(s) 394, 412, 617, 719, 1151
BsmFI GGGAC 1 cut(s) 547
BsnI GGCC 1 cut(s) 879
Bsp1286I GDGCHC 2 cut(s) 726, 1132
Bsp143I GATC 6 cut(s) 45, 391, 475, 624, 883, 1072
Bsp1720I GCTNAGC 1 cut(s) 66
BspANI GGCC 1 cut(s) 879
BspCNI CTCAG 3 cut(s) 87, 244, 1095
BspLI GGNNCC 3 cut(s) 386, 650, 717
BspPI GGATC 2 cut(s) 483, 632
BsrFI RCCGGY 1 cut(s) 712
BsrI ACTGG 3 cut(s) 541, 919, 1027
BssAI RCCGGY 1 cut(s) 712
BssECI CCNNGG 5 cut(s) 365, 453, 610, 719, 840
BssMI GATC 6 cut(s) 45, 391, 475, 624, 883, 1072
BssNI GRCGYC 1 cut(s) 167
Bst2UI CCWGG 5 cut(s) 455, 512, 612, 720, 842
Bst4CI ACNGT 3 cut(s) 581, 598, 744
Bst6I CTCTTC 1 cut(s) 72
BstACI GRCGYC 1 cut(s) 167
BstAPI GCANNNNNTGC 1 cut(s) 338
BstBAI YACGTR 1 cut(s) 1133
BstC8I GCNNGC 3 cut(s) 726, 853, 966
BstDEI CTNAG 5 cut(s) 66, 95, 252, 1006, 1082
BstDSI CCRYGG 1 cut(s) 365
BstF5I GGATG 5 cut(s) 184, 246, 316, 334, 977
BstHHI GCGC 1 cut(s) 703
BstKTI GATC 6 cut(s) 48, 394, 478, 627, 886, 1075
BstMBI GATC 6 cut(s) 45, 391, 475, 624, 883, 1072
BstMWI GCNNNNNNNGC 3 cut(s) 311, 338, 700
BstNI CCWGG 5 cut(s) 455, 512, 612, 720, 842
BstNSI RCATGY 1 cut(s) 145
BstSCI CCNGG 5 cut(s) 453, 510, 610, 718, 840
BstSLI GKGCMC 1 cut(s) 1132
BstV1I GCAGC 6 cut(s) 171, 301, 344, 383, 878, 1010
BstXI CCANNNNNNTGG 1 cut(s) 959
BsuI GTATCC 1 cut(s) 941
BsuRI GGCC 1 cut(s) 879
BtgI CCRYGG 1 cut(s) 365
BtgZI GCGATG 1 cut(s) 870
BtsCI GGATG 5 cut(s) 184, 246, 316, 334, 977
BtsIMutI CAGTG 1 cut(s) 586
Cac8I GCNNGC 3 cut(s) 726, 853, 966
CfoI GCGC 1 cut(s) 703
Cfr10I RCCGGY 1 cut(s) 712
Cfr13I GGNCC 3 cut(s) 403, 1110, 1154
CseI GACGC 2 cut(s) 175, 342
Csp6I GTAC 1 cut(s) 1086
CviAII CATG 6 cut(s) 74, 142, 389, 881, 953, 1056
CviQI GTAC 1 cut(s) 1086
DdeI CTNAG 5 cut(s) 66, 95, 252, 1006, 1082
DpnI GATC 6 cut(s) 47, 393, 477, 626, 885, 1074
DpnII GATC 6 cut(s) 45, 391, 475, 624, 883, 1072
Eam1104I CTCTTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 72
EciI GGCGGA 1 cut(s) 152
Eco24I GRGCYC 1 cut(s) 726
Eco47I GGWCC 3 cut(s) 403, 1110, 1154
Eco57I CTGAAG 1 cut(s) 645
EcoRII CCWGG 5 cut(s) 453, 510, 610, 718, 840
EcoT22I ATGCAT 1 cut(s) 958
EcoT38I GRGCYC 1 cut(s) 726
FaeI CATG 6 cut(s) 77, 145, 392, 884, 956, 1059
FaqI GGGAC 1 cut(s) 547
FatI CATG 6 cut(s) 73, 141, 388, 880, 952, 1055
FauI CCCGC 3 cut(s) 193, 844, 1117
FauNDI CATATG 1 cut(s) 958
FbaI TGATCA 2 cut(s) 391, 883
FokI GGATG 5 cut(s) 191, 253, 323, 341, 984
FriOI GRGCYC 1 cut(s) 726
FspBI CTAG 1 cut(s) 759
GlaI GCGC 1 cut(s) 702
GsaI CCCAGC 3 cut(s) 347, 640, 939
GsuI CTGGAG 3 cut(s) 533, 702, 1044
HaeIII GGCC 1 cut(s) 879
HapII CCGG 2 cut(s) 279, 713
HgaI GACGC 2 cut(s) 175, 342
HhaI GCGC 1 cut(s) 703
Hin1I GRCGYC 1 cut(s) 167
Hin1II CATG 6 cut(s) 77, 145, 392, 884, 956, 1059
Hin6I GCGC 1 cut(s) 701
HinP1I GCGC 1 cut(s) 701
HinfI GANTC 4 cut(s) 244, 398, 767, 942
HpaII CCGG 2 cut(s) 279, 713
HphI GGTGA 4 cut(s) 119, 268, 702, 878
Hpy166II GTNNAC 3 cut(s) 15, 820, 1130
Hpy188I TCNGA 6 cut(s) 45, 211, 301, 403, 475, 947
Hpy188III TCNNGA 4 cut(s) 395, 479, 499, 1108
Hpy8I GTNNAC 3 cut(s) 15, 820, 1130
Hpy99I CGWCG 3 cut(s) 169, 226, 1073
HpyAV CCTTC 3 cut(s) 410, 480, 791
HpyCH4III ACNGT 3 cut(s) 581, 598, 744
HpyCH4IV ACGT 1 cut(s) 1132
HpyCH4V TGCA 5 cut(s) 130, 145, 332, 956, 1130
HpyF10VI GCNNNNNNNGC 3 cut(s) 311, 338, 700
HpyF3I CTNAG 5 cut(s) 66, 95, 252, 1006, 1082
HpySE526I ACGT 1 cut(s) 1132
Hsp92I GRCGYC 1 cut(s) 167
Hsp92II CATG 6 cut(s) 77, 145, 392, 884, 956, 1059
HspAI GCGC 1 cut(s) 701
Ksp22I TGATCA 2 cut(s) 391, 883
Kzo9I GATC 6 cut(s) 45, 391, 475, 624, 883, 1072
LmnI GCTCC 2 cut(s) 721, 1034
Lsp1109I GCAGC 6 cut(s) 171, 301, 344, 383, 878, 1010
LweI GCATC 3 cut(s) 292, 301, 319
MaeI CTAG 1 cut(s) 759
MaeII ACGT 1 cut(s) 1132
MaeIII GTNAC 2 cut(s) 189, 744
MalI GATC 6 cut(s) 47, 393, 477, 626, 885, 1074
MboI GATC 6 cut(s) 45, 391, 475, 624, 883, 1072
MboII GAAGA 2 cut(s) 89, 823
MfeI CAATTG 1 cut(s) 348
MhlI GDGCHC 2 cut(s) 726, 1132
MluCI AATT 3 cut(s) 262, 348, 641
MlyI GAGTC 2 cut(s) 407, 761
MmeI TCCRAC 4 cut(s) 381, 381, 602, 676
Mph1103I ATGCAT 1 cut(s) 958
MseI TTAA 2 cut(s) 120, 216
MslI CAYNNNNRTG 3 cut(s) 785, 891, 957
MspA1I CMGCKG 2 cut(s) 238, 374
MspI CCGG 2 cut(s) 279, 713
MspR9I CCNGG 5 cut(s) 455, 512, 612, 720, 842
MunI CAATTG 1 cut(s) 348
MvaI CCWGG 5 cut(s) 455, 512, 612, 720, 842
MwoI GCNNNNNNNGC 3 cut(s) 311, 338, 700
NdeI CATATG 1 cut(s) 958
NdeII GATC 6 cut(s) 45, 391, 475, 624, 883, 1072
NlaIII CATG 6 cut(s) 77, 145, 392, 884, 956, 1059
NlaIV GGNNCC 3 cut(s) 386, 650, 717
NmuCI GTSAC 1 cut(s) 189
NsiI ATGCAT 1 cut(s) 958
NspI RCATGY 1 cut(s) 145
OliI CACNNNNGTG 1 cut(s) 785
PcsI WCGNNNNNNNCGW 2 cut(s) 23, 407
PfeI GAWTC 2 cut(s) 244, 942
PleI GAGTC 2 cut(s) 406, 761
PpsI GAGTC 2 cut(s) 406, 761
Ppu21I YACGTR 1 cut(s) 1133
Psp6I CCWGG 5 cut(s) 453, 510, 610, 718, 840
PspFI CCCAGC 3 cut(s) 343, 636, 935
PspGI CCWGG 5 cut(s) 453, 510, 610, 718, 840
PspN4I GGNNCC 3 cut(s) 386, 650, 717
PspPI GGNCC 3 cut(s) 403, 1110, 1154
PsrI GAACNNNNNNTAC 2 cut(s) 510, 542
PvuII CAGCTG 1 cut(s) 374
RsaI GTAC 1 cut(s) 1087
RsaNI GTAC 1 cut(s) 1086
RseI CAYNNNNRTG 3 cut(s) 785, 891, 957
SaqAI TTAA 2 cut(s) 120, 216
Sau3AI GATC 6 cut(s) 45, 391, 475, 624, 883, 1072
Sau96I GGNCC 3 cut(s) 403, 1110, 1154
ScaI AGTACT 1 cut(s) 1087
SchI GAGTC 2 cut(s) 407, 761
ScrFI CCNGG 5 cut(s) 455, 512, 612, 720, 842
SduI GDGCHC 2 cut(s) 726, 1132
SfaNI GCATC 3 cut(s) 292, 301, 319
SinI GGWCC 3 cut(s) 403, 1110, 1154
SmiMI CAYNNNNRTG 3 cut(s) 785, 891, 957
Sse9I AATT 3 cut(s) 262, 348, 641
SspMI CTAG 1 cut(s) 759
StyD4I CCNGG 5 cut(s) 453, 510, 610, 718, 840
TaaI ACNGT 3 cut(s) 581, 598, 744
TaiI ACGT 1 cut(s) 1135
TaqI TCGA 2 cut(s) 410, 1068
TasI AATT 3 cut(s) 262, 348, 641
TatI WGTACW 1 cut(s) 1085
TauI GCSGC 4 cut(s) 23, 26, 62, 65
TfiI GAWTC 2 cut(s) 244, 942
Tru1I TTAA 2 cut(s) 120, 216
Tru9I TTAA 2 cut(s) 120, 216
TscAI CASTG 1 cut(s) 586
TseFI GTSAC 1 cut(s) 189
TseI GCWGC 6 cut(s) 184, 314, 332, 371, 866, 998
Tsp45I GTSAC 1 cut(s) 189
TspDTI ATGAA 4 cut(s) 90, 256, 440, 701
TspGWI ACGGA 3 cut(s) 323, 354, 805
TspRI CASTG 1 cut(s) 586
VneI GTGCAC 1 cut(s) 1128
VpaK11BI GGWCC 3 cut(s) 403, 1110, 1154
XceI RCATGY 1 cut(s) 145
XspI CTAG 1 cut(s) 759
ZrmI AGTACT 1 cut(s) 1087
Zsp2I ATGCAT 1 cut(s) 958
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.