AT5G63220

Golgi to ER traffic protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009398
Physical Location & Seq
Reverse (-)
25353018 .. 25355535
2518 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G63220.3

Sequence Viewer

Length: 975 bp
ATGTCGAGAGAGAGGATCAAACGTGAATTACCTCCTGTTCAAGAGCATATTGATAAATTGCGGAAAGTGATAGAGGAAGGCAATTACTATGGAGCTTTGCAGATGTATAAATCTATCAGTGCAAGATATGTCACCGCTCAAAGATTCTCTGAAGCTCTTGATATCCTCTTTTCAGGGGCTTGCATTGAACTAGAACACGGCCTGGTGAATTGTGGGGCGGACCTAGCTATTTTGTTTGTTGACACACTAGTTAAAGCCAAATCTCCTTGCAACGATGAAACTCTTGATCGTATCAGGTGTATTTTCAAATTGTTTCCTCGGGTTCCTGTACCACCTCATTTGGTAGATGTGAGTGACGATGAAGATGTTCAAAATCTTCAAGAATCTCTTGGGGAAGCCAGATCACGAGTAGAAAACTTGACTTCGTTCTTGAGAGCTGCTATCAAATGGTCTGCAGAATTTGGAGGCCCACGTACTGGCTATCCTGAGTTGCATGCTATGCTGGGTGATTATCTCTACACTGAGTGTCCTGAACTTGATATGGTCCGGATATCCCGACATTTTGTTAGAGCTGAGGACCCCGAGAAATTTGCATCTATGCTGGTCAACTTTATGGGAAGGTGTTATCCCGGAGAAGATGACCTCGCAATTGCACGAGCAGTTCTCATGTATTTATCCATGGGTAACATGAAAGATGCAAACTTTATGATGGATGAGATTAAGAAACAAGCAGAGACAAAAAATCCTGAGCTTTCAGAATCGGACCTTATCCAATTTATCTCATATCTTCTGGAAACGTTGCAGAGGGATGCATTACCTCTTTTCAATATGTTAAGAGTAAAATATAAGTCAAGCATAGATAGAGATCAGCTGCTTAACGAGTTGCTGGACGAGATTGCAGAGAGGTTTTATGGTGTGCAGCGTAAGAATCCTTTGCAAGGAATGTTTGGAGACATCTTCAAGATGATGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF410

Protein Analysis

324

Amino Acids

37.18

Weight (kDa)

5.13

Isoelectric Point (pI)

41.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GET4 PF04190 13 - 286 7.2e-72 Golgi to ER traffic protein 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 476
AccBSI CCGCTC 1 cut(s) 137
AccIII TCCGGA 1 cut(s) 546
AciI CCGC 3 cut(s) 61, 135, 218
AclI AACGTT 1 cut(s) 797
AclWI GGATC 1 cut(s) 23
AcsI RAATTY 2 cut(s) 458, 587
AcuI CTGAAG 1 cut(s) 171
AdeI CACNNNGTG 1 cut(s) 525
AfaI GTAC 2 cut(s) 330, 475
AfiI CCNNNNNNNGG 2 cut(s) 476, 938
AgsI TTSAA 7 cut(s) 41, 188, 307, 371, 380, 826, 961
AhlI ACTAGT 1 cut(s) 247
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 7 cut(s) 95, 155, 227, 437, 572, 751, 871
AluI AGCT 7 cut(s) 95, 155, 227, 437, 572, 751, 871
Alw26I GTCTC 2 cut(s) 728, 945
AlwI GGATC 1 cut(s) 23
Ama87I CYCGRG 2 cut(s) 318, 581
Aor13HI TCCGGA 1 cut(s) 546
AoxI GGCC 2 cut(s) 199, 466
ApeKI GCWGC 3 cut(s) 437, 871, 919
ApoI RAATTY 2 cut(s) 458, 587
Asp700I GAANNNNTTC 1 cut(s) 366
AspS9I GGNCC 5 cut(s) 220, 467, 544, 577, 763
AsuC2I CCSGG 1 cut(s) 630
AsuHPI GGTGA 3 cut(s) 124, 217, 518
AvaI CYCGRG 2 cut(s) 318, 581
AvaII GGWCC 4 cut(s) 220, 544, 577, 763
BauI CACGAG 2 cut(s) 405, 654
BbvCI CCTCAGC 1 cut(s) 573
BbvI GCAGC 3 cut(s) 424, 858, 931
BccI CCATC 2 cut(s) 703, 961
BceAI ACGGC 1 cut(s) 214
BcgI CGANNNNNNTGC 2 cut(s) 572, 606
BciT130I CCWGG 1 cut(s) 203
BcnI CCSGG 1 cut(s) 630
BcoDI GTCTC 2 cut(s) 728, 945
BcuI ACTAGT 1 cut(s) 247
BfaI CTAG 3 cut(s) 191, 224, 248
BfmI CTRYAG 1 cut(s) 453
BisI GCNGC 3 cut(s) 438, 872, 920
BlsI GCNGC 3 cut(s) 439, 873, 921
Bme1390I CCNGG 2 cut(s) 203, 630
Bme18I GGWCC 4 cut(s) 220, 544, 577, 763
BmeT110I CYCGRG 2 cut(s) 318, 581
BmgT120I GGNCC 5 cut(s) 220, 467, 544, 577, 763
BmiI GGNNCC 2 cut(s) 324, 579
BmrFI CCNGG 2 cut(s) 203, 630
BmsI GCATC 3 cut(s) 602, 685, 799
BplI GAGNNNNNCTC 2 cut(s) 648, 680
Bpu10I CCTNAGC 2 cut(s) 573, 747
BpuEI CTTGAG 1 cut(s) 451
BpuMI CCSGG 1 cut(s) 630
BsaAI YACGTR 1 cut(s) 473
BsaBI GATNNNNATC 1 cut(s) 864
BsaJI CCNNGG 2 cut(s) 317, 678
BsaWI WCCGGW 1 cut(s) 546
Bsc4I CCNNNNNNNGG 2 cut(s) 476, 938
Bse1I ACTGG 1 cut(s) 481
Bse8I GATNNNNATC 1 cut(s) 864
BseAI TCCGGA 1 cut(s) 546
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 2 cut(s) 317, 678
BseGI GGATG 2 cut(s) 718, 814
BseJI GATNNNNATC 1 cut(s) 864
BseLI CCNNNNNNNGG 2 cut(s) 476, 938
BseMII CTCAG 4 cut(s) 477, 513, 564, 738
BseNI ACTGG 1 cut(s) 481
BseXI GCAGC 3 cut(s) 424, 858, 931
BseYI CCCAGC 1 cut(s) 502
BsgI GTGCAG 1 cut(s) 938
BshFI GGCC 2 cut(s) 201, 468
BsiHKCI CYCGRG 2 cut(s) 318, 581
BsiSI CCGG 2 cut(s) 547, 630
BslI CCNNNNNNNGG 2 cut(s) 476, 938
BsmAI GTCTC 2 cut(s) 728, 945
BsnI GGCC 2 cut(s) 201, 468
BsoBI CYCGRG 2 cut(s) 318, 581
Bsp13I TCCGGA 1 cut(s) 546
Bsp143I GATC 4 cut(s) 15, 286, 401, 865
Bsp19I CCATGG 1 cut(s) 678
BspACI CCGC 3 cut(s) 61, 135, 218
BspANI GGCC 2 cut(s) 201, 468
BspCNI CTCAG 4 cut(s) 478, 514, 565, 739
BspEI TCCGGA 1 cut(s) 546
BspLI GGNNCC 2 cut(s) 324, 579
BspMAI CTGCAG 1 cut(s) 457
BspPI GGATC 1 cut(s) 23
BsrBI CCGCTC 1 cut(s) 137
BsrI ACTGG 1 cut(s) 481
BssECI CCNNGG 2 cut(s) 317, 678
BssMI GATC 4 cut(s) 15, 286, 401, 865
BssSI CACGAG 2 cut(s) 405, 654
BssT1I CCWWGG 1 cut(s) 678
Bst2BI CACGAG 2 cut(s) 405, 654
Bst2UI CCWGG 1 cut(s) 203
BstAPI GCANNNNNTGC 1 cut(s) 499
BstBAI YACGTR 1 cut(s) 473
BstC8I GCNNGC 2 cut(s) 181, 495
BstDEI CTNAG 4 cut(s) 486, 522, 573, 747
BstDSI CCRYGG 1 cut(s) 678
BstENI CCTNNNNNAGG 1 cut(s) 936
BstF5I GGATG 2 cut(s) 718, 814
BstKTI GATC 4 cut(s) 18, 289, 404, 868
BstMAI GTCTC 2 cut(s) 728, 945
BstMBI GATC 4 cut(s) 15, 286, 401, 865
BstMWI GCNNNNNNNGC 2 cut(s) 224, 499
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 1 cut(s) 497
BstSCI CCNGG 2 cut(s) 201, 628
BstSFI CTRYAG 1 cut(s) 453
BstV1I GCAGC 3 cut(s) 424, 858, 931
BsuRI GGCC 2 cut(s) 201, 468
BtgI CCRYGG 1 cut(s) 678
BtsCI GGATG 2 cut(s) 718, 814
BtsIMutI CAGTG 2 cut(s) 124, 519
Cac8I GCNNGC 2 cut(s) 181, 495
Cfr13I GGNCC 5 cut(s) 220, 467, 544, 577, 763
Csp6I GTAC 2 cut(s) 329, 474
CspCI CAANNNNNGTGG 2 cut(s) 321, 356
CviAII CATG 4 cut(s) 494, 667, 679, 688
CviQI GTAC 2 cut(s) 329, 474
DdeI CTNAG 4 cut(s) 486, 522, 573, 747
DpnI GATC 4 cut(s) 17, 288, 403, 867
DpnII GATC 4 cut(s) 15, 286, 401, 865
DraIII CACNNNGTG 1 cut(s) 525
EciI GGCGGA 1 cut(s) 233
Eco130I CCWWGG 1 cut(s) 678
Eco32I GATATC 2 cut(s) 163, 552
Eco47I GGWCC 4 cut(s) 220, 544, 577, 763
Eco57I CTGAAG 1 cut(s) 171
Eco88I CYCGRG 2 cut(s) 318, 581
EcoNI CCTNNNNNAGG 1 cut(s) 936
EcoO109I RGGNCCY 1 cut(s) 577
EcoRII CCWGG 1 cut(s) 201
EcoRV GATATC 2 cut(s) 163, 552
EcoT14I CCWWGG 1 cut(s) 678
EcoT22I ATGCAT 1 cut(s) 814
ErhI CCWWGG 1 cut(s) 678
FaeI CATG 4 cut(s) 497, 670, 682, 691
FalI AAGNNNNNCTT 2 cut(s) 372, 404
FatI CATG 4 cut(s) 493, 666, 678, 687
Fnu4HI GCNGC 3 cut(s) 438, 872, 920
FokI GGATG 2 cut(s) 725, 821
Fsp4HI GCNGC 3 cut(s) 438, 872, 920
FspBI CTAG 3 cut(s) 191, 224, 248
GluI GCNGC 3 cut(s) 438, 872, 920
GsaI CCCAGC 1 cut(s) 506
HaeIII GGCC 2 cut(s) 201, 468
HapII CCGG 2 cut(s) 547, 630
Hin1II CATG 4 cut(s) 497, 670, 682, 691
HincII GTYRAC 2 cut(s) 241, 607
HindII GTYRAC 2 cut(s) 241, 607
HinfI GANTC 4 cut(s) 144, 383, 758, 928
HpaII CCGG 2 cut(s) 547, 630
HphI GGTGA 3 cut(s) 124, 217, 518
Hpy166II GTNNAC 2 cut(s) 241, 607
Hpy188I TCNGA 3 cut(s) 151, 757, 763
Hpy8I GTNNAC 2 cut(s) 241, 607
HpyAV CCTTC 2 cut(s) 71, 612
HpyCH4IV ACGT 3 cut(s) 22, 472, 797
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 499
HpyF3I CTNAG 4 cut(s) 486, 522, 573, 747
HpySE526I ACGT 3 cut(s) 22, 472, 797
Hsp92II CATG 4 cut(s) 497, 670, 682, 691
Kpn2I TCCGGA 1 cut(s) 546
Kzo9I GATC 4 cut(s) 15, 286, 401, 865
LmnI GCTCC 1 cut(s) 92
Lsp1109I GCAGC 3 cut(s) 424, 858, 931
LweI GCATC 3 cut(s) 602, 685, 799
MaeI CTAG 3 cut(s) 191, 224, 248
MaeII ACGT 3 cut(s) 22, 472, 797
MaeIII GTNAC 3 cut(s) 130, 353, 683
MalI GATC 4 cut(s) 17, 288, 403, 867
MbiI CCGCTC 1 cut(s) 137
MboI GATC 4 cut(s) 15, 286, 401, 865
MboII GAAGA 5 cut(s) 368, 374, 647, 779, 949
MfeI CAATTG 1 cut(s) 648
MluCI AATT 9 cut(s) 26, 56, 82, 208, 308, 458, 587, 648, 773
Mph1103I ATGCAT 1 cut(s) 814
MroI TCCGGA 1 cut(s) 546
MroXI GAANNNNTTC 1 cut(s) 366
MseI TTAA 4 cut(s) 252, 720, 833, 876
MspA1I CMGCKG 1 cut(s) 871
MspI CCGG 2 cut(s) 547, 630
MspR9I CCNGG 2 cut(s) 203, 630
MunI CAATTG 1 cut(s) 648
MvaI CCWGG 1 cut(s) 203
MwoI GCNNNNNNNGC 2 cut(s) 224, 499
NciI CCSGG 1 cut(s) 630
NcoI CCATGG 1 cut(s) 678
NdeII GATC 4 cut(s) 15, 286, 401, 865
NlaIII CATG 4 cut(s) 497, 670, 682, 691
NlaIV GGNNCC 2 cut(s) 324, 579
NmuCI GTSAC 2 cut(s) 130, 353
NsiI ATGCAT 1 cut(s) 814
NspI RCATGY 1 cut(s) 497
PaeI GCATGC 1 cut(s) 497
PdmI GAANNNNTTC 1 cut(s) 366
PfeI GAWTC 4 cut(s) 144, 383, 758, 928
PflMI CCANNNNNTGG 1 cut(s) 476
PfoI TCCNGGA 1 cut(s) 628
PkrI GCNGC 3 cut(s) 439, 873, 921
Ppu21I YACGTR 1 cut(s) 473
PpuMI RGGWCCY 1 cut(s) 577
Psp1406I AACGTT 1 cut(s) 797
Psp5II RGGWCCY 1 cut(s) 577
Psp6I CCWGG 1 cut(s) 201
PspFI CCCAGC 1 cut(s) 502
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 2 cut(s) 324, 579
PspPI GGNCC 5 cut(s) 220, 467, 544, 577, 763
PspPPI RGGWCCY 1 cut(s) 577
PstI CTGCAG 1 cut(s) 457
PvuII CAGCTG 1 cut(s) 871
RsaI GTAC 2 cut(s) 330, 475
RsaNI GTAC 2 cut(s) 329, 474
SaqAI TTAA 4 cut(s) 252, 720, 833, 876
SatI GCNGC 3 cut(s) 438, 872, 920
Sau3AI GATC 4 cut(s) 15, 286, 401, 865
Sau96I GGNCC 5 cut(s) 220, 467, 544, 577, 763
ScrFI CCNGG 2 cut(s) 203, 630
SfaNI GCATC 3 cut(s) 602, 685, 799
SfcI CTRYAG 1 cut(s) 453
SinI GGWCC 4 cut(s) 220, 544, 577, 763
SmlI CTYRAG 1 cut(s) 430
SmoI CTYRAG 1 cut(s) 430
SpeI ACTAGT 1 cut(s) 247
SphI GCATGC 1 cut(s) 497
Sse9I AATT 9 cut(s) 26, 56, 82, 208, 308, 458, 587, 648, 773
SsiI CCGC 3 cut(s) 61, 135, 218
SspMI CTAG 3 cut(s) 191, 224, 248
StyD4I CCNGG 2 cut(s) 201, 628
StyI CCWWGG 1 cut(s) 678
TaiI ACGT 3 cut(s) 25, 475, 800
TaqI TCGA 1 cut(s) 5
TasI AATT 9 cut(s) 26, 56, 82, 208, 308, 458, 587, 648, 773
TfiI GAWTC 4 cut(s) 144, 383, 758, 928
Tru1I TTAA 4 cut(s) 252, 720, 833, 876
Tru9I TTAA 4 cut(s) 252, 720, 833, 876
TscAI CASTG 2 cut(s) 124, 526
TseFI GTSAC 2 cut(s) 130, 353
TseI GCWGC 3 cut(s) 437, 871, 919
Tsp45I GTSAC 2 cut(s) 130, 353
TspDTI ATGAA 3 cut(s) 291, 375, 704
TspRI CASTG 2 cut(s) 124, 526
Van91I CCANNNNNTGG 1 cut(s) 476
VpaK11BI GGWCC 4 cut(s) 220, 544, 577, 763
XagI CCTNNNNNAGG 1 cut(s) 936
XapI RAATTY 2 cut(s) 458, 587
XceI RCATGY 1 cut(s) 497
XmnI GAANNNNTTC 1 cut(s) 366
XspI CTAG 3 cut(s) 191, 224, 248
Zsp2I ATGCAT 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.