MD07G1179200.v1.1

Golgi to ER traffic protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009398
Physical Location & Seq
Reverse (-)
25882479 .. 25887939
5461 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1179200.v1.1.491

Sequence Viewer

Length: 981 bp
ATGTCGCGGGAGAGACCCAAAAAAGGCGCACTGCCTCCCGCCCAAGAGAATATTGAGAAATTAGAGAAAGTTGTAGAGGAAGGTAATTACTATGGAGCTCAGCAAATGTACAAATCTATTAGTGCAAGATATGTAGCTGCTCAGAGGTTTTCTGAGGCTGTGGATATCCTTCAGTCTGGCGCATGCAACCAACTGAAACATGGGCAGGTTACTTGTGGTGCTGAGTTGGCTGCTTTGTTTGTGGAAACACTTGTCAAAGGAAGATTCCCTTATGACGATGAAACACTAGGTCGTGTCAGGAAAATTTATAAAGCATTTCCTCGGATACCTATGCCGCAGCAGTTAGGGGATTTAGATGACATGCAACAACTTTCTGAAACACTTGGGGAAGCAAAAACATGTGTTGAGGGCTGCTCATCGTTTCTAAAATCTGCTCTTAAGTGGTCTAAGGAGTTTGGTGCACCCAAGGGTGGAGCTCCAGAAATACATGTCATGTTAGCGAACTACATATATTTTGAATCTCCTGAGGTGGACATGGTTAGAGTGTCGTATCATTTTGTTCGAGGAAACAACCCCAAAGAGTTTGCTTCGACTTTAGTAAATTTTATGGGCAAGTGTTATCCAGGGGAAGATGATTTGGCCATTGCACGAGCGATTTTAATGTATTTGGCAGTTGGTAATCTGAGAGATGCCAATATTCTCGTGGATGAGATAAAGAAGCAAGTGGAGTCTAAGCAGCTCGACTTTCCCAAATCCAATTTGATTCAGTTCATCAATTTTCTTTTGCAAACGTTGCTGAGAGATGCTCTTCCGCTTTTTAATATGTTGAGAGAGAAATACAAATCAAGCTTGGACAGAGAACCAACATTTCATGAGTTGCTGGATGAAATTGCGGAGAAGTTTTATGGAGTACGGCGCAGAAATCCGCTGCAAGGGATGGGAATGTTTGGCGAGATTTTCAAGATGATGGGGGGTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF410

Protein Analysis

327

Amino Acids

36.92

Weight (kDa)

6.49

Isoelectric Point (pI)

44.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GET4 PF04190 48 - 320 3e-68 Golgi to ER traffic protein 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 309
Acc36I ACCTGC 1 cut(s) 196
AccII CGCG 1 cut(s) 7
AciI CCGC 6 cut(s) 7, 39, 335, 812, 893, 926
AclI AACGTT 1 cut(s) 791
AcoI YGGCCR 1 cut(s) 639
AcsI RAATTY 2 cut(s) 303, 601
AcuI CTGAAG 1 cut(s) 155
AfaI GTAC 2 cut(s) 110, 912
AfiI CCNNNNNNNGG 3 cut(s) 23, 470, 932
AflII CTTAAG 1 cut(s) 437
AflIII ACRYGT 2 cut(s) 398, 487
AgsI TTSAA 2 cut(s) 518, 961
AjnI CCWGG 1 cut(s) 622
AluBI AGCT 5 cut(s) 98, 137, 476, 739, 849
AluI AGCT 5 cut(s) 98, 137, 476, 739, 849
Alw21I GWGCWC 3 cut(s) 100, 463, 478
Alw26I GTCTC 1 cut(s) 7
Alw44I GTGCAC 1 cut(s) 459
AoxI GGCC 1 cut(s) 639
ApaLI GTGCAC 1 cut(s) 459
ApeKI GCWGC 6 cut(s) 137, 230, 337, 411, 736, 928
ApoI RAATTY 2 cut(s) 303, 601
AspLEI GCGC 3 cut(s) 29, 182, 918
AxyI CCTNAGG 1 cut(s) 525
BaeGI GKGCMC 1 cut(s) 463
BalI TGGCCA 1 cut(s) 641
BanII GRGCYC 2 cut(s) 100, 478
BauI CACGAG 2 cut(s) 648, 701
Bbv12I GWGCWC 3 cut(s) 100, 463, 478
BbvI GCAGC 6 cut(s) 124, 217, 349, 398, 748, 915
BccI CCATC 2 cut(s) 931, 961
BceAI ACGGC 1 cut(s) 929
BciT130I CCWGG 1 cut(s) 624
BciVI GTATCC 1 cut(s) 318
BcoDI GTCTC 1 cut(s) 7
BfaI CTAG 1 cut(s) 287
BfrI CTTAAG 1 cut(s) 437
BfuAI ACCTGC 1 cut(s) 196
BfuI GTATCC 1 cut(s) 318
BisI GCNGC 7 cut(s) 138, 231, 335, 338, 412, 737, 929
BlpI GCTNAGC 1 cut(s) 99
BlsI GCNGC 7 cut(s) 139, 232, 336, 339, 413, 738, 930
Bme1390I CCNGG 1 cut(s) 624
BmrFI CCNGG 1 cut(s) 624
BmsI GCATC 2 cut(s) 679, 793
BplI GAGNNNNNCTC 4 cut(s) 398, 430, 790, 822
BpmI CTGGAG 1 cut(s) 462
Bpu1102I GCTNAGC 1 cut(s) 99
BsaI GGTCTC 1 cut(s) 7
BsaJI CCNNGG 3 cut(s) 320, 465, 623
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 470, 932
Bse21I CCTNAGG 1 cut(s) 525
Bse3DI GCAATG 1 cut(s) 642
BseBI CCWGG 1 cut(s) 624
BseDI CCNNGG 3 cut(s) 320, 465, 623
BseGI GGATG 3 cut(s) 712, 889, 942
BseLI CCNNNNNNNGG 3 cut(s) 23, 470, 932
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 7 cut(s) 113, 144, 155, 213, 516, 674, 788
BseSI GKGCMC 1 cut(s) 463
BseXI GCAGC 6 cut(s) 124, 217, 349, 398, 748, 915
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 1 cut(s) 641
BsiHKAI GWGCWC 3 cut(s) 100, 463, 478
BslI CCNNNNNNNGG 3 cut(s) 23, 470, 932
BsmAI GTCTC 1 cut(s) 7
BsnI GGCC 1 cut(s) 641
Bso31I GGTCTC 1 cut(s) 7
Bsp1286I GDGCHC 3 cut(s) 100, 463, 478
Bsp1407I TGTACA 1 cut(s) 108
Bsp1720I GCTNAGC 1 cut(s) 99
BspACI CCGC 6 cut(s) 7, 39, 335, 812, 893, 926
BspANI GGCC 1 cut(s) 641
BspCNI CTCAG 7 cut(s) 112, 145, 154, 214, 517, 675, 789
BspFNI CGCG 1 cut(s) 7
BspHI TCATGA 1 cut(s) 871
BspMI ACCTGC 1 cut(s) 196
BspQI GCTCTTC 1 cut(s) 813
BspTI CTTAAG 1 cut(s) 437
BspTNI GGTCTC 1 cut(s) 7
BsrDI GCAATG 1 cut(s) 642
BsrGI TGTACA 1 cut(s) 108
BssECI CCNNGG 3 cut(s) 320, 465, 623
BssSI CACGAG 2 cut(s) 648, 701
BssT1I CCWWGG 1 cut(s) 465
Bst2BI CACGAG 2 cut(s) 648, 701
Bst2UI CCWGG 1 cut(s) 624
Bst6I CTCTTC 1 cut(s) 813
BstAFI CTTAAG 1 cut(s) 437
BstAPI GCANNNNNTGC 1 cut(s) 793
BstAUI TGTACA 1 cut(s) 108
BstC8I GCNNGC 1 cut(s) 184
BstDEI CTNAG 9 cut(s) 99, 141, 153, 222, 447, 525, 683, 732, 797
BstF5I GGATG 3 cut(s) 712, 889, 942
BstFNI CGCG 1 cut(s) 7
BstHHI GCGC 3 cut(s) 29, 182, 918
BstMAI GTCTC 1 cut(s) 7
BstMWI GCNNNNNNNGC 2 cut(s) 227, 793
BstNI CCWGG 1 cut(s) 624
BstNSI RCATGY 4 cut(s) 186, 364, 402, 491
BstSCI CCNGG 1 cut(s) 622
BstSLI GKGCMC 1 cut(s) 463
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 6 cut(s) 124, 217, 349, 398, 748, 915
Bsu36I CCTNAGG 1 cut(s) 525
BsuI GTATCC 1 cut(s) 318
BsuRI GGCC 1 cut(s) 641
BtsCI GGATG 3 cut(s) 712, 889, 942
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 29
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 1 cut(s) 184
CciI TCATGA 1 cut(s) 871
CfoI GCGC 3 cut(s) 29, 182, 918
Csp6I GTAC 2 cut(s) 109, 911
CviAII CATG 8 cut(s) 183, 200, 361, 399, 488, 493, 535, 872
CviJI RGCY 9 cut(s) 98, 137, 158, 230, 411, 476, 641, 739, 849
CviKI_1 RGCY 9 cut(s) 98, 137, 158, 230, 411, 476, 641, 739, 849
CviQI GTAC 2 cut(s) 109, 911
DdeI CTNAG 9 cut(s) 99, 141, 153, 222, 447, 525, 683, 732, 797
EaeI YGGCCR 1 cut(s) 639
Eam1104I CTCTTC 1 cut(s) 813
EarI CTCTTC 1 cut(s) 813
Ecl136II GAGCTC 2 cut(s) 98, 476
Eco130I CCWWGG 1 cut(s) 465
Eco24I GRGCYC 2 cut(s) 100, 478
Eco31I GGTCTC 1 cut(s) 7
Eco32I GATATC 1 cut(s) 166
Eco53kI GAGCTC 2 cut(s) 98, 476
Eco57I CTGAAG 1 cut(s) 155
Eco81I CCTNAGG 1 cut(s) 525
EcoICRI GAGCTC 2 cut(s) 98, 476
EcoRII CCWGG 1 cut(s) 622
EcoRV GATATC 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 465
EcoT38I GRGCYC 2 cut(s) 100, 478
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 8 cut(s) 186, 203, 364, 402, 491, 496, 538, 875
FalI AAGNNNNNCTT 2 cut(s) 253, 285
FatI CATG 8 cut(s) 182, 199, 360, 398, 487, 492, 534, 871
FauI CCCGC 1 cut(s) 46
Fnu4HI GCNGC 7 cut(s) 138, 231, 335, 338, 412, 737, 929
FokI GGATG 3 cut(s) 719, 896, 949
FriOI GRGCYC 2 cut(s) 100, 478
Fsp4HI GCNGC 7 cut(s) 138, 231, 335, 338, 412, 737, 929
FspBI CTAG 1 cut(s) 287
GlaI GCGC 3 cut(s) 28, 181, 917
GluI GCNGC 7 cut(s) 138, 231, 335, 338, 412, 737, 929
GsuI CTGGAG 1 cut(s) 462
HaeIII GGCC 1 cut(s) 641
HhaI GCGC 3 cut(s) 29, 182, 918
Hin1II CATG 8 cut(s) 186, 203, 364, 402, 491, 496, 538, 875
Hin6I GCGC 3 cut(s) 27, 180, 916
HinP1I GCGC 3 cut(s) 27, 180, 916
HindIII AAGCTT 1 cut(s) 847
HinfI GANTC 4 cut(s) 264, 518, 728, 763
Hpy166II GTNNAC 2 cut(s) 461, 532
Hpy188I TCNGA 5 cut(s) 144, 154, 324, 376, 684
Hpy188III TCNNGA 5 cut(s) 298, 479, 524, 872, 961
Hpy8I GTNNAC 2 cut(s) 461, 532
HpyAV CCTTC 2 cut(s) 74, 179
HpyCH4IV ACGT 1 cut(s) 791
HpyCH4V TGCA 7 cut(s) 125, 186, 364, 461, 647, 787, 931
HpyF10VI GCNNNNNNNGC 2 cut(s) 227, 793
HpyF3I CTNAG 9 cut(s) 99, 141, 153, 222, 447, 525, 683, 732, 797
HpySE526I ACGT 1 cut(s) 791
Hsp92II CATG 8 cut(s) 186, 203, 364, 402, 491, 496, 538, 875
HspAI GCGC 3 cut(s) 27, 180, 916
LguI GCTCTTC 1 cut(s) 813
LmnI GCTCC 3 cut(s) 95, 473, 481
LpnPI CCDG 8 cut(s) 162, 191, 283, 492, 537, 609, 636, 866
Lsp1109I GCAGC 6 cut(s) 124, 217, 349, 398, 748, 915
LweI GCATC 2 cut(s) 679, 793
MaeI CTAG 1 cut(s) 287
MaeII ACGT 1 cut(s) 791
MaeIII GTNAC 1 cut(s) 208
MboII GAAGA 3 cut(s) 273, 641, 800
MhlI GDGCHC 3 cut(s) 100, 463, 478
MlsI TGGCCA 1 cut(s) 641
MluCI AATT 7 cut(s) 59, 85, 303, 601, 757, 775, 888
MluNI TGGCCA 1 cut(s) 641
MlyI GAGTC 1 cut(s) 737
MnlI CCTC 8 cut(s) 45, 70, 138, 148, 330, 400, 520, 557
Mox20I TGGCCA 1 cut(s) 641
MscI TGGCCA 1 cut(s) 641
MseI TTAA 3 cut(s) 438, 659, 819
Msp20I TGGCCA 1 cut(s) 641
MspA1I CMGCKG 1 cut(s) 928
MspCI CTTAAG 1 cut(s) 437
MspR9I CCNGG 1 cut(s) 624
MvaI CCWGG 1 cut(s) 624
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 2 cut(s) 227, 793
NlaIII CATG 8 cut(s) 186, 203, 364, 402, 491, 496, 538, 875
NspI RCATGY 4 cut(s) 186, 364, 402, 491
PaeI GCATGC 1 cut(s) 186
PagI TCATGA 1 cut(s) 871
PciI ACATGT 2 cut(s) 398, 487
PciSI GCTCTTC 1 cut(s) 813
PfeI GAWTC 3 cut(s) 264, 518, 763
PkrI GCNGC 7 cut(s) 139, 232, 336, 339, 413, 738, 930
PleI GAGTC 1 cut(s) 736
PpsI GAGTC 1 cut(s) 736
PscI ACATGT 2 cut(s) 398, 487
PsiI TTATAA 1 cut(s) 309
Psp124BI GAGCTC 2 cut(s) 100, 478
Psp1406I AACGTT 1 cut(s) 791
Psp6I CCWGG 1 cut(s) 622
PspGI CCWGG 1 cut(s) 622
RsaI GTAC 2 cut(s) 110, 912
RsaNI GTAC 2 cut(s) 109, 911
SacI GAGCTC 2 cut(s) 100, 478
SapI GCTCTTC 1 cut(s) 813
SaqAI TTAA 3 cut(s) 438, 659, 819
SatI GCNGC 7 cut(s) 138, 231, 335, 338, 412, 737, 929
SchI GAGTC 1 cut(s) 737
ScrFI CCNGG 1 cut(s) 624
SduI GDGCHC 3 cut(s) 100, 463, 478
SfaNI GCATC 2 cut(s) 679, 793
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
SphI GCATGC 1 cut(s) 186
Sse9I AATT 7 cut(s) 59, 85, 303, 601, 757, 775, 888
SsiI CCGC 6 cut(s) 7, 39, 335, 812, 893, 926
SspI AATATT 2 cut(s) 52, 697
SspMI CTAG 1 cut(s) 287
SstI GAGCTC 2 cut(s) 100, 478
StyD4I CCNGG 1 cut(s) 622
StyI CCWWGG 1 cut(s) 465
TaiI ACGT 1 cut(s) 794
TaqI TCGA 3 cut(s) 562, 590, 741
TasI AATT 7 cut(s) 59, 85, 303, 601, 757, 775, 888
TatI WGTACW 1 cut(s) 108
TauI GCSGC 1 cut(s) 337
TfiI GAWTC 3 cut(s) 264, 518, 763
Tru1I TTAA 3 cut(s) 438, 659, 819
Tru9I TTAA 3 cut(s) 438, 659, 819
TscAI CASTG 1 cut(s) 36
TseI GCWGC 6 cut(s) 137, 230, 337, 411, 736, 928
TspDTI ATGAA 4 cut(s) 294, 760, 860, 900
TspRI CASTG 1 cut(s) 36
Vha464I CTTAAG 1 cut(s) 437
VneI GTGCAC 1 cut(s) 459
XapI RAATTY 2 cut(s) 303, 601
XceI RCATGY 4 cut(s) 186, 364, 402, 491
XcmI CCANNNNNNNNNTGG 2 cut(s) 197, 700
XspI CTAG 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.