MD01G1131100.v1.1

Golgi to ER traffic protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009398
Physical Location & Seq
Reverse (-)
24167526 .. 24172727
5202 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1131100.v1.1.491

Sequence Viewer

Length: 981 bp
ATGTCGCGGGAGAGACCCAAAAAAGGCGCATTGCCTCCCGCCCAGGAGAATATTCAGAAATTAGAGAAAGTTGTAGATGAAGGTAATTTCTATGGAGCTCAACAAATGTACAAATCTATTAGTGCAAGATATGTAGCTGCTCAGAGGTTTTCCGAGGCTGTGGATATCCTTCAGTCCGGTGCATGCAACCAACTGAAACATGGGCAGGTTACTTGTGGTGCTGAGTTAGCTGCTTTGTTTGTGGAAACACTTGTCAAGGGAAGATTCCCTTATGACGATGAAACCTTAGTTCGTGTCAGGAAAATTTATAAAGCATTTCCTCGGATACCTGTGCCGCAACAGTTAGGGGATTTAGATGACATGCAACAACTGTCTGAAACACTTGGGGCAGCAAAAACATGTGTTGAGGGCTGCTCATCATTCTTAAAGGCTGCTCTTAAGTGGTCTAAGGAGTTTGGTGCACCCAGGAGTGGAGCTCCAGAAATACACGTTATGCTAGCAAACTACATATATTTTGAATCTCCTGAGGTGGACATGGTTAGAGTGTCACATCATTTTGTTAGAGGAAACAACCCCAAAGAGTTTGCTTTGACTTTAGTAAATTTTATGGGCAAGTGTTATCCAGAGGAAGATGATTTGGCCATTGCCCGAGCGATTTTAATGTATTTGTCAGTTGGTAATCTGAGAGATGCCAATATTCTCAGGGATGAGATAAAGAAGCAAGTGGAGTCTAAGCAGCTTGACTTTCCCATATCCGATTTGATCCAGTTCATCAATTTTCTTTTGCAAACGTTGTTGAGAGATGCTCTTCCACTTTTTAATATGTTGAGAGCGAAATACAAATCAAGCCTAGACAGAGAACCAACATTTCATGAGTTGCTGGATGAAATTGCGGAGAAGTTTTATGGAGTACGACGCAGAAATCCACTTCAAGGGATGGGGATGTTCGGCGAGATGTTCAAGATGATGGGAGGTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF410

Protein Analysis

327

Amino Acids

36.98

Weight (kDa)

6.97

Isoelectric Point (pI)

44.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GET4 PF04190 48 - 320 3.1e-66 Golgi to ER traffic protein 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 309
Acc36I ACCTGC 1 cut(s) 196
AccII CGCG 1 cut(s) 7
AciI CCGC 4 cut(s) 7, 39, 335, 893
AclI AACGTT 1 cut(s) 791
AclWI GGATC 1 cut(s) 757
AcoI YGGCCR 1 cut(s) 639
AcsI RAATTY 2 cut(s) 303, 601
AcuI CTGAAG 1 cut(s) 155
AfaI GTAC 2 cut(s) 110, 912
AfiI CCNNNNNNNGG 3 cut(s) 23, 470, 932
AflII CTTAAG 1 cut(s) 437
AflIII ACRYGT 2 cut(s) 398, 487
AgsI TTSAA 3 cut(s) 518, 932, 961
AjnI CCWGG 2 cut(s) 42, 464
AluBI AGCT 5 cut(s) 98, 137, 230, 476, 739
AluI AGCT 5 cut(s) 98, 137, 230, 476, 739
Alw21I GWGCWC 3 cut(s) 100, 463, 478
Alw26I GTCTC 1 cut(s) 7
Alw44I GTGCAC 1 cut(s) 459
AlwI GGATC 1 cut(s) 757
Ama87I CYCGRG 1 cut(s) 648
AoxI GGCC 1 cut(s) 639
ApaLI GTGCAC 1 cut(s) 459
ApeKI GCWGC 6 cut(s) 137, 230, 389, 411, 431, 736
ApoI RAATTY 2 cut(s) 303, 601
AspLEI GCGC 1 cut(s) 29
AsuNHI GCTAGC 1 cut(s) 496
AvaI CYCGRG 1 cut(s) 648
AxyI CCTNAGG 1 cut(s) 525
BaeGI GKGCMC 1 cut(s) 463
BalI TGGCCA 1 cut(s) 641
BanII GRGCYC 2 cut(s) 100, 478
Bbv12I GWGCWC 3 cut(s) 100, 463, 478
BbvI GCAGC 6 cut(s) 124, 217, 398, 401, 418, 748
BccI CCATC 2 cut(s) 931, 961
BciT130I CCWGG 2 cut(s) 44, 466
BciVI GTATCC 1 cut(s) 318
BcoDI GTCTC 1 cut(s) 7
BfaI CTAG 2 cut(s) 497, 851
BfrI CTTAAG 1 cut(s) 437
BfuAI ACCTGC 1 cut(s) 196
BfuI GTATCC 1 cut(s) 318
BisI GCNGC 7 cut(s) 138, 231, 335, 390, 412, 432, 737
BlsI GCNGC 7 cut(s) 139, 232, 336, 391, 413, 433, 738
Bme1390I CCNGG 2 cut(s) 44, 466
BmeT110I CYCGRG 1 cut(s) 648
BmrFI CCNGG 2 cut(s) 44, 466
BmsI GCATC 2 cut(s) 679, 793
BmtI GCTAGC 1 cut(s) 500
BplI GAGNNNNNCTC 6 cut(s) 398, 430, 460, 492, 790, 822
BpmI CTGGAG 1 cut(s) 462
BsaI GGTCTC 1 cut(s) 7
BsaJI CCNNGG 4 cut(s) 42, 153, 320, 464
BsaWI WCCGGW 1 cut(s) 176
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 470, 932
Bse1I ACTGG 1 cut(s) 766
Bse21I CCTNAGG 1 cut(s) 525
Bse3DI GCAATG 2 cut(s) 29, 642
BseBI CCWGG 2 cut(s) 44, 466
BseDI CCNNGG 4 cut(s) 42, 153, 320, 464
BseGI GGATG 4 cut(s) 712, 889, 942, 948
BseLI CCNNNNNNNGG 3 cut(s) 23, 470, 932
BseMI GCAATG 2 cut(s) 29, 642
BseMII CTCAG 5 cut(s) 155, 213, 516, 674, 715
BseNI ACTGG 1 cut(s) 766
BseSI GKGCMC 1 cut(s) 463
BseXI GCAGC 6 cut(s) 124, 217, 398, 401, 418, 748
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 1 cut(s) 641
BsiHKAI GWGCWC 3 cut(s) 100, 463, 478
BsiHKCI CYCGRG 1 cut(s) 648
BsiSI CCGG 1 cut(s) 177
BslI CCNNNNNNNGG 3 cut(s) 23, 470, 932
BsmAI GTCTC 1 cut(s) 7
BsnI GGCC 1 cut(s) 641
Bso31I GGTCTC 1 cut(s) 7
BsoBI CYCGRG 1 cut(s) 648
Bsp1286I GDGCHC 3 cut(s) 100, 463, 478
Bsp1407I TGTACA 1 cut(s) 108
Bsp143I GATC 1 cut(s) 762
BspACI CCGC 4 cut(s) 7, 39, 335, 893
BspANI GGCC 1 cut(s) 641
BspCNI CTCAG 5 cut(s) 154, 214, 517, 675, 714
BspFNI CGCG 1 cut(s) 7
BspHI TCATGA 1 cut(s) 871
BspMI ACCTGC 1 cut(s) 196
BspOI GCTAGC 1 cut(s) 500
BspPI GGATC 1 cut(s) 757
BspQI GCTCTTC 1 cut(s) 813
BspTI CTTAAG 1 cut(s) 437
BspTNI GGTCTC 1 cut(s) 7
BsrDI GCAATG 2 cut(s) 29, 642
BsrGI TGTACA 1 cut(s) 108
BsrI ACTGG 1 cut(s) 766
BssECI CCNNGG 4 cut(s) 42, 153, 320, 464
BssMI GATC 1 cut(s) 762
Bst2UI CCWGG 2 cut(s) 44, 466
Bst4CI ACNGT 2 cut(s) 342, 372
Bst6I CTCTTC 1 cut(s) 813
BstAFI CTTAAG 1 cut(s) 437
BstAUI TGTACA 1 cut(s) 108
BstC8I GCNNGC 2 cut(s) 184, 498
BstDEI CTNAG 8 cut(s) 141, 222, 286, 447, 525, 683, 701, 732
BstF5I GGATG 4 cut(s) 712, 889, 942, 948
BstFNI CGCG 1 cut(s) 7
BstHHI GCGC 1 cut(s) 29
BstKTI GATC 1 cut(s) 765
BstMAI GTCTC 1 cut(s) 7
BstMBI GATC 1 cut(s) 762
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstNI CCWGG 2 cut(s) 44, 466
BstNSI RCATGY 3 cut(s) 186, 364, 402
BstSCI CCNGG 2 cut(s) 42, 464
BstSLI GKGCMC 1 cut(s) 463
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 6 cut(s) 124, 217, 398, 401, 418, 748
Bsu36I CCTNAGG 1 cut(s) 525
BsuI GTATCC 1 cut(s) 318
BsuRI GGCC 1 cut(s) 641
BtsCI GGATG 4 cut(s) 712, 889, 942, 948
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 2 cut(s) 184, 498
CciI TCATGA 1 cut(s) 871
CfoI GCGC 1 cut(s) 29
CseI GACGC 1 cut(s) 924
Csp6I GTAC 2 cut(s) 109, 911
CviAII CATG 6 cut(s) 183, 200, 361, 399, 535, 872
CviQI GTAC 2 cut(s) 109, 911
DdeI CTNAG 8 cut(s) 141, 222, 286, 447, 525, 683, 701, 732
DpnI GATC 1 cut(s) 764
DpnII GATC 1 cut(s) 762
EaeI YGGCCR 1 cut(s) 639
Eam1104I CTCTTC 1 cut(s) 813
EarI CTCTTC 1 cut(s) 813
Ecl136II GAGCTC 2 cut(s) 98, 476
Eco24I GRGCYC 2 cut(s) 100, 478
Eco31I GGTCTC 1 cut(s) 7
Eco32I GATATC 1 cut(s) 166
Eco53kI GAGCTC 2 cut(s) 98, 476
Eco57I CTGAAG 1 cut(s) 155
Eco81I CCTNAGG 1 cut(s) 525
Eco88I CYCGRG 1 cut(s) 648
EcoICRI GAGCTC 2 cut(s) 98, 476
EcoRII CCWGG 2 cut(s) 42, 464
EcoRV GATATC 1 cut(s) 166
EcoT38I GRGCYC 2 cut(s) 100, 478
FaeI CATG 6 cut(s) 186, 203, 364, 402, 538, 875
FalI AAGNNNNNCTT 2 cut(s) 253, 285
FatI CATG 6 cut(s) 182, 199, 360, 398, 534, 871
FauI CCCGC 1 cut(s) 46
Fnu4HI GCNGC 7 cut(s) 138, 231, 335, 390, 412, 432, 737
FokI GGATG 4 cut(s) 719, 896, 949, 955
FriOI GRGCYC 2 cut(s) 100, 478
Fsp4HI GCNGC 7 cut(s) 138, 231, 335, 390, 412, 432, 737
FspBI CTAG 2 cut(s) 497, 851
GlaI GCGC 1 cut(s) 28
GluI GCNGC 7 cut(s) 138, 231, 335, 390, 412, 432, 737
GsuI CTGGAG 1 cut(s) 462
HaeIII GGCC 1 cut(s) 641
HapII CCGG 1 cut(s) 177
HgaI GACGC 1 cut(s) 924
HhaI GCGC 1 cut(s) 29
Hin1II CATG 6 cut(s) 186, 203, 364, 402, 538, 875
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HinfI GANTC 3 cut(s) 264, 518, 728
HpaII CCGG 1 cut(s) 177
Hpy166II GTNNAC 2 cut(s) 461, 532
Hpy188I TCNGA 7 cut(s) 57, 144, 154, 324, 376, 684, 757
Hpy188III TCNNGA 6 cut(s) 298, 479, 524, 623, 872, 961
Hpy8I GTNNAC 2 cut(s) 461, 532
Hpy99I CGWCG 1 cut(s) 918
HpyAV CCTTC 2 cut(s) 74, 179
HpyCH4III ACNGT 2 cut(s) 342, 372
HpyCH4IV ACGT 2 cut(s) 489, 791
HpyCH4V TGCA 6 cut(s) 125, 182, 186, 364, 461, 787
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpyF3I CTNAG 8 cut(s) 141, 222, 286, 447, 525, 683, 701, 732
HpySE526I ACGT 2 cut(s) 489, 791
Hsp92II CATG 6 cut(s) 186, 203, 364, 402, 538, 875
HspAI GCGC 1 cut(s) 27
Kzo9I GATC 1 cut(s) 762
LguI GCTCTTC 1 cut(s) 813
LmnI GCTCC 3 cut(s) 95, 473, 481
Lsp1109I GCAGC 6 cut(s) 124, 217, 398, 401, 418, 748
LweI GCATC 2 cut(s) 679, 793
MaeI CTAG 2 cut(s) 497, 851
MaeII ACGT 2 cut(s) 489, 791
MaeIII GTNAC 2 cut(s) 208, 546
MalI GATC 1 cut(s) 764
MboI GATC 1 cut(s) 762
MboII GAAGA 3 cut(s) 273, 641, 800
MhlI GDGCHC 3 cut(s) 100, 463, 478
MlsI TGGCCA 1 cut(s) 641
MluCI AATT 6 cut(s) 59, 85, 303, 601, 775, 888
MluNI TGGCCA 1 cut(s) 641
MlyI GAGTC 1 cut(s) 737
MnlI CCTC 9 cut(s) 45, 138, 148, 330, 400, 520, 557, 619, 965
Mox20I TGGCCA 1 cut(s) 641
MscI TGGCCA 1 cut(s) 641
MseI TTAA 4 cut(s) 425, 438, 659, 819
Msp20I TGGCCA 1 cut(s) 641
MspCI CTTAAG 1 cut(s) 437
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 2 cut(s) 44, 466
MvaI CCWGG 2 cut(s) 44, 466
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 1 cut(s) 227
NdeII GATC 1 cut(s) 762
NheI GCTAGC 1 cut(s) 496
NlaIII CATG 6 cut(s) 186, 203, 364, 402, 538, 875
NmuCI GTSAC 1 cut(s) 546
NspI RCATGY 3 cut(s) 186, 364, 402
PaeI GCATGC 1 cut(s) 186
PagI TCATGA 1 cut(s) 871
PciI ACATGT 1 cut(s) 398
PciSI GCTCTTC 1 cut(s) 813
PfeI GAWTC 2 cut(s) 264, 518
PkrI GCNGC 7 cut(s) 139, 232, 336, 391, 413, 433, 738
PleI GAGTC 1 cut(s) 736
PpsI GAGTC 1 cut(s) 736
PscI ACATGT 1 cut(s) 398
PsiI TTATAA 1 cut(s) 309
Psp124BI GAGCTC 2 cut(s) 100, 478
Psp1406I AACGTT 1 cut(s) 791
Psp6I CCWGG 2 cut(s) 42, 464
PspGI CCWGG 2 cut(s) 42, 464
RsaI GTAC 2 cut(s) 110, 912
RsaNI GTAC 2 cut(s) 109, 911
SacI GAGCTC 2 cut(s) 100, 478
SapI GCTCTTC 1 cut(s) 813
SaqAI TTAA 4 cut(s) 425, 438, 659, 819
SatI GCNGC 7 cut(s) 138, 231, 335, 390, 412, 432, 737
Sau3AI GATC 1 cut(s) 762
SchI GAGTC 1 cut(s) 737
ScrFI CCNGG 2 cut(s) 44, 466
SduI GDGCHC 3 cut(s) 100, 463, 478
SfaNI GCATC 2 cut(s) 679, 793
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
SphI GCATGC 1 cut(s) 186
Sse9I AATT 6 cut(s) 59, 85, 303, 601, 775, 888
SsiI CCGC 4 cut(s) 7, 39, 335, 893
SspI AATATT 2 cut(s) 52, 697
SspMI CTAG 2 cut(s) 497, 851
SstI GAGCTC 2 cut(s) 100, 478
StyD4I CCNGG 2 cut(s) 42, 464
TaaI ACNGT 2 cut(s) 342, 372
TaiI ACGT 2 cut(s) 492, 794
TasI AATT 6 cut(s) 59, 85, 303, 601, 775, 888
TatI WGTACW 1 cut(s) 108
TauI GCSGC 1 cut(s) 337
TfiI GAWTC 2 cut(s) 264, 518
Tru1I TTAA 4 cut(s) 425, 438, 659, 819
Tru9I TTAA 4 cut(s) 425, 438, 659, 819
TseFI GTSAC 1 cut(s) 546
TseI GCWGC 6 cut(s) 137, 230, 389, 411, 431, 736
Tsp45I GTSAC 1 cut(s) 546
TspDTI ATGAA 5 cut(s) 93, 294, 760, 860, 900
Vha464I CTTAAG 1 cut(s) 437
VneI GTGCAC 1 cut(s) 459
XapI RAATTY 2 cut(s) 303, 601
XceI RCATGY 3 cut(s) 186, 364, 402
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XspI CTAG 2 cut(s) 497, 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.