Rw1G028470

Golgi to ER traffic protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0009398
Physical Location & Seq
Reverse (-)
56155373 .. 56159633
4261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G028470.1

Sequence Viewer

Length: 981 bp
ATGTCGAAGGAGAGAGCCAAAAGAGGCACACTGCCTCCAGCCCAAGAGCAAATTGAGAAATTTGAGAAAGTTGTAGAGGAAGGTAACTACTATGGAGCTCAGCAGCTGTACAAATCTATTAGTGCGAGATATGTGGCTGCTCAGAGGTATACTGAAGCTTTAGATATTCTTCAGTCGGGTGCATGCATCCAGTTGAAACATGGGCAGGTTACGTGTGGGGCGGAGTTAGCTGCTTTGTTTGTGGATACACTTGTCAAAGCGAGATGCCCTTATGACGATGAAACCCTCGTTCGTGTCAGGACAATTTATAAACAGTTTCCTAAGATACCTGTGCCACAACAGTTGGGGGATGATGATGACGTGCAACGACTTTCTGAAACACTTGGGACAGCAAAAACATGTGTTGAAGGATGCTCATCATTCCTAAAAGCTGCTATCAAGTGGTCTGCGGAGTTTGGTGCACCTAGGGGTGGGGCTCCAGAAATACATGTTATGCTAGCTAACTATATGTACTTTGAATCTTCTGAGGTGGACATGGTTAGAGTTGCACAGCATTTTGTCAGAGGAAACAACCCAAAAGAATTTGCTTCTACTTTAGTAGATTTCATGGGCAAGTGCTATCCAGGGGAAGATGATGTGGCCATTGCAAGGGCAATTTTAATGTATTTGGCTTTAGGTAACCTGAGAGATGCCAATGCTCTCATGGATGAGATAAAAAAGCAAGTAGAAGCTAAGCAGCTTGAATTTCCTAAATCCGATTTGATGCAGTTCATTAATTTTCTTTTGGAAACGATGCTGAGAGATGCGTTTCCTCTTTTTAATATGTTGAGATCGAATTTCAAATCAACCTTAGACAGAGAGCCTAGTTTTCACGAGTTGCTAGATGAAATTGCAGAGAAGTTTTATGGAGTAAAGTGCAGAAATCCTCTACAAGGGATGGGAATGTTTGGGGAGATCTTCAAGATGATGGGAGGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF410

Protein Analysis

326

Amino Acids

36.74

Weight (kDa)

5.48

Isoelectric Point (pI)

37.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GET4 PF04190 48 - 320 5.8e-67 Golgi to ER traffic protein 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 309
Acc36I ACCTGC 1 cut(s) 196
AccI GTMKAC 1 cut(s) 149
AciI CCGC 2 cut(s) 221, 449
AcoI YGGCCR 1 cut(s) 639
AcsI RAATTY 4 cut(s) 59, 581, 743, 835
AcuI CTGAAG 2 cut(s) 155, 174
AfaI GTAC 2 cut(s) 110, 512
AfiI CCNNNNNNNGG 3 cut(s) 470, 648, 932
AflIII ACRYGT 3 cut(s) 212, 398, 487
AgsI TTSAA 6 cut(s) 196, 407, 518, 743, 841, 961
AjiI CACGTC 1 cut(s) 361
AjnI CCWGG 1 cut(s) 622
AluBI AGCT 8 cut(s) 98, 106, 158, 230, 431, 500, 731, 739
AluI AGCT 8 cut(s) 98, 106, 158, 230, 431, 500, 731, 739
Alw21I GWGCWC 2 cut(s) 100, 463
Alw44I GTGCAC 1 cut(s) 459
AlwNI CAGNNNCTG 1 cut(s) 106
AoxI GGCC 1 cut(s) 639
ApaLI GTGCAC 1 cut(s) 459
ApeKI GCWGC 5 cut(s) 103, 137, 230, 431, 736
ApoI RAATTY 4 cut(s) 59, 581, 743, 835
AseI ATTAAT 1 cut(s) 774
AspA2I CCTAGG 1 cut(s) 464
AsuNHI GCTAGC 1 cut(s) 496
AvrII CCTAGG 1 cut(s) 464
BaeGI GKGCMC 1 cut(s) 463
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BalI TGGCCA 1 cut(s) 641
BanII GRGCYC 2 cut(s) 100, 478
BauI CACGAG 1 cut(s) 872
Bbv12I GWGCWC 2 cut(s) 100, 463
BbvI GCAGC 5 cut(s) 115, 124, 217, 418, 748
BccI CCATC 2 cut(s) 931, 961
BciT130I CCWGG 1 cut(s) 624
BciVI GTATCC 1 cut(s) 238
BfaI CTAG 4 cut(s) 465, 497, 864, 881
BfuAI ACCTGC 1 cut(s) 196
BfuI GTATCC 1 cut(s) 238
BglII AGATCT 1 cut(s) 954
BisI GCNGC 5 cut(s) 104, 138, 231, 432, 737
BlnI CCTAGG 1 cut(s) 464
BlpI GCTNAGC 2 cut(s) 99, 732
BlsI GCNGC 5 cut(s) 105, 139, 232, 433, 738
Bme1390I CCNGG 1 cut(s) 624
BmgBI CACGTC 1 cut(s) 361
BmiI GGNNCC 1 cut(s) 477
BmrFI CCNGG 1 cut(s) 624
BmsI GCATC 7 cut(s) 195, 254, 401, 679, 753, 783, 793
BmtI GCTAGC 1 cut(s) 500
BpmI CTGGAG 2 cut(s) 21, 462
Bpu1102I GCTNAGC 2 cut(s) 99, 732
BsaAI YACGTR 1 cut(s) 213
BsaBI GATNNNNATC 1 cut(s) 415
BsaJI CCNNGG 2 cut(s) 464, 623
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 3 cut(s) 470, 648, 932
Bse1I ACTGG 1 cut(s) 190
Bse3DI GCAATG 1 cut(s) 642
Bse8I GATNNNNATC 1 cut(s) 415
BseBI CCWGG 1 cut(s) 624
BseDI CCNNGG 2 cut(s) 464, 623
BseGI GGATG 5 cut(s) 186, 355, 416, 712, 942
BseJI GATNNNNATC 1 cut(s) 415
BseLI CCNNNNNNNGG 3 cut(s) 470, 648, 932
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 5 cut(s) 113, 155, 516, 674, 788
BseNI ACTGG 1 cut(s) 190
BseSI GKGCMC 1 cut(s) 463
BseXI GCAGC 5 cut(s) 115, 124, 217, 418, 748
BsgI GTGCAG 1 cut(s) 937
BshFI GGCC 1 cut(s) 641
BsiHKAI GWGCWC 2 cut(s) 100, 463
BslFI GGGAC 1 cut(s) 400
BslI CCNNNNNNNGG 3 cut(s) 470, 648, 932
BsmFI GGGAC 1 cut(s) 400
BsnI GGCC 1 cut(s) 641
Bsp1286I GDGCHC 3 cut(s) 100, 463, 478
Bsp1407I TGTACA 1 cut(s) 108
Bsp143I GATC 2 cut(s) 830, 954
Bsp1720I GCTNAGC 2 cut(s) 99, 732
BspACI CCGC 2 cut(s) 221, 449
BspANI GGCC 1 cut(s) 641
BspCNI CTCAG 5 cut(s) 112, 154, 517, 675, 789
BspLI GGNNCC 1 cut(s) 477
BspMI ACCTGC 1 cut(s) 196
BspOI GCTAGC 1 cut(s) 500
BsrDI GCAATG 1 cut(s) 642
BsrGI TGTACA 1 cut(s) 108
BsrI ACTGG 1 cut(s) 190
BssECI CCNNGG 2 cut(s) 464, 623
BssMI GATC 2 cut(s) 830, 954
BssNAI GTATAC 1 cut(s) 150
BssSI CACGAG 1 cut(s) 872
BssT1I CCWWGG 1 cut(s) 464
Bst1107I GTATAC 1 cut(s) 150
Bst2BI CACGAG 1 cut(s) 872
Bst2UI CCWGG 1 cut(s) 624
Bst4CI ACNGT 2 cut(s) 315, 342
BstAUI TGTACA 1 cut(s) 108
BstBAI YACGTR 1 cut(s) 213
BstC8I GCNNGC 2 cut(s) 184, 498
BstDEI CTNAG 8 cut(s) 99, 141, 321, 525, 683, 732, 797, 850
BstEII GGTNACC 1 cut(s) 677
BstENI CCTNNNNNAGG 1 cut(s) 930
BstF5I GGATG 5 cut(s) 186, 355, 416, 712, 942
BstKTI GATC 2 cut(s) 833, 957
BstMBI GATC 2 cut(s) 830, 954
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstNI CCWGG 1 cut(s) 624
BstNSI RCATGY 3 cut(s) 186, 402, 491
BstPI GGTNACC 1 cut(s) 677
BstSCI CCNGG 1 cut(s) 622
BstSLI GKGCMC 1 cut(s) 463
BstV1I GCAGC 5 cut(s) 115, 124, 217, 418, 748
BstX2I RGATCY 1 cut(s) 954
BstYI RGATCY 1 cut(s) 954
BstZ17I GTATAC 1 cut(s) 150
BsuI GTATCC 1 cut(s) 238
BsuRI GGCC 1 cut(s) 641
BtrI CACGTC 1 cut(s) 361
BtsCI GGATG 5 cut(s) 186, 355, 416, 712, 942
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 29
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 2 cut(s) 184, 498
CaiI CAGNNNCTG 1 cut(s) 106
Csp6I GTAC 2 cut(s) 109, 511
CspCI CAANNNNNGTGG 2 cut(s) 324, 359
CviAII CATG 7 cut(s) 183, 200, 399, 488, 535, 607, 703
CviQI GTAC 2 cut(s) 109, 511
DdeI CTNAG 8 cut(s) 99, 141, 321, 525, 683, 732, 797, 850
DpnI GATC 2 cut(s) 832, 956
DpnII GATC 2 cut(s) 830, 954
EaeI YGGCCR 1 cut(s) 639
EciI GGCGGA 1 cut(s) 236
Ecl136II GAGCTC 1 cut(s) 98
Eco130I CCWWGG 1 cut(s) 464
Eco24I GRGCYC 2 cut(s) 100, 478
Eco53kI GAGCTC 1 cut(s) 98
Eco57I CTGAAG 2 cut(s) 155, 174
Eco91I GGTNACC 1 cut(s) 677
EcoICRI GAGCTC 1 cut(s) 98
EcoNI CCTNNNNNAGG 1 cut(s) 930
EcoO65I GGTNACC 1 cut(s) 677
EcoRII CCWGG 1 cut(s) 622
EcoT14I CCWWGG 1 cut(s) 464
EcoT22I ATGCAT 1 cut(s) 188
EcoT38I GRGCYC 2 cut(s) 100, 478
ErhI CCWWGG 1 cut(s) 464
FaeI CATG 7 cut(s) 186, 203, 402, 491, 538, 610, 706
FaqI GGGAC 1 cut(s) 400
FatI CATG 7 cut(s) 182, 199, 398, 487, 534, 606, 702
FblI GTMKAC 1 cut(s) 149
Fnu4HI GCNGC 5 cut(s) 104, 138, 231, 432, 737
FokI GGATG 5 cut(s) 173, 362, 423, 719, 949
FriOI GRGCYC 2 cut(s) 100, 478
Fsp4HI GCNGC 5 cut(s) 104, 138, 231, 432, 737
FspBI CTAG 4 cut(s) 465, 497, 864, 881
GluI GCNGC 5 cut(s) 104, 138, 231, 432, 737
GsuI CTGGAG 2 cut(s) 21, 462
HaeIII GGCC 1 cut(s) 641
Hin1II CATG 7 cut(s) 186, 203, 402, 491, 538, 610, 706
HindIII AAGCTT 1 cut(s) 156
HinfI GANTC 1 cut(s) 518
Hpy166II GTNNAC 3 cut(s) 150, 461, 532
Hpy188I TCNGA 5 cut(s) 144, 376, 526, 563, 757
Hpy188III TCNNGA 4 cut(s) 298, 479, 872, 961
Hpy8I GTNNAC 3 cut(s) 150, 461, 532
HpyAV CCTTC 2 cut(s) 74, 401
HpyCH4III ACNGT 2 cut(s) 315, 342
HpyCH4IV ACGT 2 cut(s) 212, 360
HpyCH4V TGCA 9 cut(s) 182, 186, 364, 461, 548, 647, 766, 893, 918
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpyF3I CTNAG 8 cut(s) 99, 141, 321, 525, 683, 732, 797, 850
HpySE526I ACGT 2 cut(s) 212, 360
Hsp92II CATG 7 cut(s) 186, 203, 402, 491, 538, 610, 706
Kzo9I GATC 2 cut(s) 830, 954
LmnI GCTCC 2 cut(s) 95, 481
LpnPI CCDG 9 cut(s) 51, 191, 203, 283, 342, 492, 609, 636, 695
Lsp1109I GCAGC 5 cut(s) 115, 124, 217, 418, 748
LweI GCATC 7 cut(s) 195, 254, 401, 679, 753, 783, 793
MaeI CTAG 4 cut(s) 465, 497, 864, 881
MaeII ACGT 2 cut(s) 212, 360
MaeIII GTNAC 3 cut(s) 83, 208, 677
MalI GATC 2 cut(s) 832, 956
MboI GATC 2 cut(s) 830, 954
MboII GAAGA 4 cut(s) 161, 513, 641, 949
MflI RGATCY 1 cut(s) 954
MhlI GDGCHC 3 cut(s) 100, 463, 478
MlsI TGGCCA 1 cut(s) 641
MluCI AATT 9 cut(s) 51, 59, 303, 581, 654, 743, 775, 835, 888
MluNI TGGCCA 1 cut(s) 641
Mox20I TGGCCA 1 cut(s) 641
Mph1103I ATGCAT 1 cut(s) 188
MscI TGGCCA 1 cut(s) 641
MseI TTAA 3 cut(s) 659, 774, 819
Msp20I TGGCCA 1 cut(s) 641
MspA1I CMGCKG 1 cut(s) 106
MspR9I CCNGG 1 cut(s) 624
MvaI CCWGG 1 cut(s) 624
MwoI GCNNNNNNNGC 1 cut(s) 227
NdeII GATC 2 cut(s) 830, 954
NheI GCTAGC 1 cut(s) 496
NlaIII CATG 7 cut(s) 186, 203, 402, 491, 538, 610, 706
NlaIV GGNNCC 1 cut(s) 477
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 3 cut(s) 186, 402, 491
PaeI GCATGC 1 cut(s) 186
PciI ACATGT 2 cut(s) 398, 487
PfeI GAWTC 1 cut(s) 518
PkrI GCNGC 5 cut(s) 105, 139, 232, 433, 738
Ppu21I YACGTR 1 cut(s) 213
PscI ACATGT 2 cut(s) 398, 487
PshBI ATTAAT 1 cut(s) 774
PsiI TTATAA 1 cut(s) 309
Psp124BI GAGCTC 1 cut(s) 100
Psp6I CCWGG 1 cut(s) 622
PspEI GGTNACC 1 cut(s) 677
PspGI CCWGG 1 cut(s) 622
PspN4I GGNNCC 1 cut(s) 477
PstNI CAGNNNCTG 1 cut(s) 106
PsuI RGATCY 1 cut(s) 954
PvuII CAGCTG 1 cut(s) 106
RsaI GTAC 2 cut(s) 110, 512
RsaNI GTAC 2 cut(s) 109, 511
SacI GAGCTC 1 cut(s) 100
SaqAI TTAA 3 cut(s) 659, 774, 819
SatI GCNGC 5 cut(s) 104, 138, 231, 432, 737
Sau3AI GATC 2 cut(s) 830, 954
ScrFI CCNGG 1 cut(s) 624
SduI GDGCHC 3 cut(s) 100, 463, 478
SfaNI GCATC 7 cut(s) 195, 254, 401, 679, 753, 783, 793
SphI GCATGC 1 cut(s) 186
Sse9I AATT 9 cut(s) 51, 59, 303, 581, 654, 743, 775, 835, 888
SsiI CCGC 2 cut(s) 221, 449
SspMI CTAG 4 cut(s) 465, 497, 864, 881
SstI GAGCTC 1 cut(s) 100
StyD4I CCNGG 1 cut(s) 622
StyI CCWWGG 1 cut(s) 464
TaaI ACNGT 2 cut(s) 315, 342
TaiI ACGT 2 cut(s) 215, 363
TaqI TCGA 2 cut(s) 5, 833
TasI AATT 9 cut(s) 51, 59, 303, 581, 654, 743, 775, 835, 888
TatI WGTACW 2 cut(s) 108, 510
TfiI GAWTC 1 cut(s) 518
Tru1I TTAA 3 cut(s) 659, 774, 819
Tru9I TTAA 3 cut(s) 659, 774, 819
TscAI CASTG 1 cut(s) 36
TseI GCWGC 5 cut(s) 103, 137, 230, 431, 736
TspDTI ATGAA 4 cut(s) 294, 595, 760, 900
TspRI CASTG 1 cut(s) 36
VneI GTGCAC 1 cut(s) 459
VspI ATTAAT 1 cut(s) 774
XagI CCTNNNNNAGG 1 cut(s) 930
XapI RAATTY 4 cut(s) 59, 581, 743, 835
XceI RCATGY 3 cut(s) 186, 402, 491
XcmI CCANNNNNNNNNTGG 2 cut(s) 197, 700
XmaJI CCTAGG 1 cut(s) 464
XmiI GTMKAC 1 cut(s) 149
XspI CTAG 4 cut(s) 465, 497, 864, 881
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.