AT5G64090

Hyccin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014272
Physical Location & Seq
Forward (+)
25647913 .. 25649768
1856 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G64090.1

Sequence Viewer

Length: 1347 bp
ATGGACTTCTCCGTCAAACCTTCCGGCGGCTCACCTTCTCCGTCATCTTCCACCTCTTCTTCTACGCCTCATCGTTTCAAATCCGTTACCACACCAACCGCTACTGCCGCTGCCGTCTCAGGATTCTCCCCCTCCGCCGCCGCTGATCGAGATCCGATGCATTCCTGGTGGGAATCAGTCTCAAAGCAACGTTCTCGCATCCTCTCTCTTTCATCTCTTCTCTCCGGCGATTCTCACTTTGAAGACGGTGACGTAACTCCGATCTCCTCTCTTGCAGATTCCGATCGGCCGGCGTTATCGTTACTCTCTTCTCGTGCTGCTTACTCTTTGATATCGAATTCTCTTTGCAATCCAGCTTCTGGTTCTGGATCTGATCCGCTTTGTCAATGGCTATACGAAACTTATCTCTCCTCTGATCCACCACTTCGTCTCGTTGTCCTCTCGTTCTTTCCGTTACTCGTCGGAATGTACTTGTCTCGAATTCATTCCTCGGATTCGACATCTCTTCCTTCTCTATCCGGATTCGAAGCTGTGCTTCTCGCAATCTACGCCGCTGAGGTTAAAGCTCGCGCAGGTAAACCTATACTCGTACACATTCCAGATCTCTCTCAGCCATCTCTTTACCACACACCGAGAAACGGCGTTGACAAATCGCGTGATTCCAATCCCACCGCCTCCGTCGGAGTCTTGTCTCCGCAGCTTGAGCCTCAGATCGCGGTTAAGTCAACTAAGCGAGCCAGCATCGTTGGCGTGGGACTACAATGCTATTTCAAGGAGATCTCACAAATGCCGGCTTGGTCTAAGCTTGAATTCTGTAAGTTCTCTGCTAGTTGGGCTGGTCAAGACTGCGATTGCAAGGAAAAGATCGATGAAGACGAAGACAAAGTCTTAGCACTAACTAATGGTTTTGGAGATTCATCCTCTTTCAATGGAAGCAGTGGACGTAGTCTTGAGATCGAAGAGGATTTTGATAGATTAGCAATCAGAGAGAACGAAGAACAACTTAGCTCTAATGGCGGAGGAGGGGGAGTAGGAAGAGGCGTGAGGATTCCACTTCCATGGGAGCTGTTTCAACCTACGTTAAGGATTCTAGGTCATTGCTTGCTTAGTCCATTGAATACTGAAGATGTTAAAGATGCAGCTTCCAATGCAGTAAGATCATTGTACGCAAGAGCCTCTCATGATCTGAATCCACAGGCGATTTTAGCTACAAGGAGTTTAGTTAACCTTGATACAAGCGCACGAACCTCGGGAAAGACCGTAGCTGCAGAGACTGTTAATGGCTCATCGAATGTGAACACTCCAAGCAAGGCGAAAAAACCTGAGATTCTTCTGGCATCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hyccin

Protein Analysis

448

Amino Acids

47.72

Weight (kDa)

5.95

Isoelectric Point (pI)

49.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hyccin PF09790 93 - 417 6.2e-68 Hyccin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64090
fragaria_vesca FvH4_1g19990
malus_domestica MD05G1042400.v1.1 MD10G1049000.v1.1
prunus_persica Prupe.8G059200_v2.0.a1
pyrus_communis pycom05g03500
rosa_chinensis RchiOBHm_Chr2g0111931
rosa_laevigata RLG00000017939 RLG00000034552
rosa_multiflora Rmu_sc0005679.1_g000010
rosa_roxburghii Rroxscaffold_2G00131980
rosa_rugosa Rorug02G0177300
rosa_samantha Rh2AG229700 Rh2BG242400 Rh2CG233800 Rh2DG237400
rosa_wichuraiana Rw2G017770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 11
Acc36I ACCTGC 1 cut(s) 563
AccB7I CCANNNNNTGG 1 cut(s) 359
AccII CGCG 3 cut(s) 570, 655, 716
AccIII TCCGGA 1 cut(s) 518
AclI AACGTT 1 cut(s) 190
AclWI GGATC 4 cut(s) 146, 368, 376, 410
AcoI YGGCCR 1 cut(s) 287
AcsI RAATTY 3 cut(s) 337, 480, 809
AcuI CTGAAG 1 cut(s) 1143
AfaI GTAC 3 cut(s) 470, 591, 1166
AfiI CCNNNNNNNGG 3 cut(s) 26, 359, 638
AgsI TTSAA 7 cut(s) 79, 242, 772, 809, 928, 1073, 1117
AjnI CCWGG 1 cut(s) 164
Alw26I GTCTC 6 cut(s) 121, 184, 434, 480, 696, 1265
AlwI GGATC 4 cut(s) 146, 368, 376, 410
AlwNI CAGNNNCTG 2 cut(s) 359, 1274
Ama87I CYCGRG 1 cut(s) 1249
Aor13HI TCCGGA 1 cut(s) 518
AoxI GGCC 1 cut(s) 287
ApeKI GCWGC 5 cut(s) 110, 317, 697, 1139, 1265
ApoI RAATTY 3 cut(s) 337, 480, 809
ArsI GACNNNNNNTTYG 2 cut(s) 870, 902
Asp700I GAANNNNTTC 1 cut(s) 484
AspLEI GCGC 2 cut(s) 572, 1241
AsuHPI GGTGA 2 cut(s) 24, 260
AsuII TTCGAA 1 cut(s) 525
AvaI CYCGRG 1 cut(s) 1249
BauI CACGAG 1 cut(s) 312
BbsI GAAGAC 3 cut(s) 249, 879, 885
BbvCI CCTCAGC 1 cut(s) 555
BbvI GCAGC 5 cut(s) 97, 304, 709, 1151, 1252
BccI CCATC 1 cut(s) 622
BceAI ACGGC 2 cut(s) 98, 655
BcgI CGANNNNNNTGC 4 cut(s) 176, 210, 1230, 1264
BciT130I CCWGG 1 cut(s) 166
BcoDI GTCTC 6 cut(s) 121, 184, 434, 480, 696, 1265
BfaI CTAG 2 cut(s) 828, 1091
BfmI CTRYAG 1 cut(s) 1266
BfuAI ACCTGC 1 cut(s) 563
BglII AGATCT 2 cut(s) 601, 777
Bme1390I CCNGG 1 cut(s) 166
BmeT110I CYCGRG 1 cut(s) 1249
BmrFI CCNGG 1 cut(s) 166
BmsI GCATC 4 cut(s) 147, 207, 750, 1126
BpiI GAAGAC 3 cut(s) 249, 879, 885
Bpu10I CCTNAGC 1 cut(s) 555
Bpu14I TTCGAA 1 cut(s) 525
BpuEI CTTGAG 2 cut(s) 722, 971
Bsa29I ATCGAT 1 cut(s) 867
BsaBI GATNNNNATC 4 cut(s) 150, 282, 663, 1188
BsaJI CCNNGG 3 cut(s) 489, 1058, 1248
BsaWI WCCGGW 1 cut(s) 518
BsaXI ACNNNNNCTCC 2 cut(s) 1014, 1044
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 359, 638
Bse118I RCCGGY 2 cut(s) 289, 790
Bse3DI GCAATG 1 cut(s) 1096
Bse8I GATNNNNATC 4 cut(s) 150, 282, 663, 1188
BseAI TCCGGA 1 cut(s) 518
BseBI CCWGG 1 cut(s) 166
BseCI ATCGAT 1 cut(s) 867
BseDI CCNNGG 3 cut(s) 489, 1058, 1248
BseGI GGATG 2 cut(s) 198, 917
BseJI GATNNNNATC 4 cut(s) 150, 282, 663, 1188
BseLI CCNNNNNNNGG 3 cut(s) 26, 359, 638
BseMI GCAATG 1 cut(s) 1096
BseMII CTCAG 5 cut(s) 132, 546, 623, 722, 1314
BseRI GAGGAG 3 cut(s) 256, 400, 1035
BseX3I CGGCCG 1 cut(s) 287
BseXI GCAGC 5 cut(s) 97, 304, 709, 1151, 1252
Bsh1236I CGCG 3 cut(s) 570, 655, 716
Bsh1285I CGRYCG 2 cut(s) 286, 290
BshFI GGCC 1 cut(s) 289
BshVI ATCGAT 1 cut(s) 867
BsiEI CGRYCG 2 cut(s) 286, 290
BsiHKCI CYCGRG 1 cut(s) 1249
BsiSI CCGG 5 cut(s) 24, 225, 290, 519, 791
BslFI GGGAC 1 cut(s) 768
BslI CCNNNNNNNGG 3 cut(s) 26, 359, 638
BsmAI GTCTC 6 cut(s) 121, 184, 434, 480, 696, 1265
BsmBI CGTCTC 2 cut(s) 121, 434
BsmFI GGGAC 1 cut(s) 768
BsmI GAATGC 1 cut(s) 160
BsnI GGCC 1 cut(s) 289
BsoBI CYCGRG 1 cut(s) 1249
Bsp119I TTCGAA 1 cut(s) 525
Bsp13I TCCGGA 1 cut(s) 518
Bsp19I CCATGG 1 cut(s) 1058
BspANI GGCC 1 cut(s) 289
BspCNI CTCAG 5 cut(s) 131, 547, 622, 721, 1315
BspDI ATCGAT 1 cut(s) 867
BspEI TCCGGA 1 cut(s) 518
BspFNI CGCG 3 cut(s) 570, 655, 716
BspHI TCATGA 1 cut(s) 1180
BspMAI CTGCAG 1 cut(s) 1270
BspMI ACCTGC 1 cut(s) 563
BspPI GGATC 4 cut(s) 146, 368, 376, 410
BspT104I TTCGAA 1 cut(s) 525
BsrDI GCAATG 1 cut(s) 1096
BsrFI RCCGGY 2 cut(s) 289, 790
BssAI RCCGGY 2 cut(s) 289, 790
BssECI CCNNGG 3 cut(s) 489, 1058, 1248
BssSI CACGAG 1 cut(s) 312
BssT1I CCWWGG 1 cut(s) 1058
Bst2BI CACGAG 1 cut(s) 312
Bst2UI CCWGG 1 cut(s) 166
Bst4CI ACNGT 3 cut(s) 248, 1261, 1276
Bst6I CTCTTC 6 cut(s) 61, 222, 313, 510, 954, 1030
BstBI TTCGAA 1 cut(s) 525
BstC8I GCNNGC 6 cut(s) 291, 568, 735, 739, 792, 1103
BstDSI CCRYGG 1 cut(s) 1058
BstF5I GGATG 2 cut(s) 198, 917
BstFNI CGCG 3 cut(s) 570, 655, 716
BstHHI GCGC 2 cut(s) 572, 1241
BstMAI GTCTC 6 cut(s) 121, 184, 434, 480, 696, 1265
BstMCI CGRYCG 2 cut(s) 286, 290
BstMWI GCNNNNNNNGC 8 cut(s) 107, 548, 703, 747, 833, 1014, 1148, 1205
BstNI CCWGG 1 cut(s) 166
BstSCI CCNGG 1 cut(s) 164
BstSFI CTRYAG 1 cut(s) 1266
BstUI CGCG 3 cut(s) 570, 655, 716
BstV1I GCAGC 5 cut(s) 97, 304, 709, 1151, 1252
BstV2I GAAGAC 3 cut(s) 249, 879, 885
BstX2I RGATCY 4 cut(s) 151, 368, 601, 777
BstXI CCANNNNNNTGG 1 cut(s) 1059
BstYI RGATCY 4 cut(s) 151, 368, 601, 777
BstZI CGGCCG 1 cut(s) 287
Bsu15I ATCGAT 1 cut(s) 867
BsuRI GGCC 1 cut(s) 289
BsuTUI ATCGAT 1 cut(s) 867
BtgI CCRYGG 1 cut(s) 1058
BtsCI GGATG 2 cut(s) 198, 917
BtsI GCAGTG 1 cut(s) 943
BtsIMutI CAGTG 1 cut(s) 943
BveI ACCTGC 1 cut(s) 563
Cac8I GCNNGC 6 cut(s) 291, 568, 735, 739, 792, 1103
CaiI CAGNNNCTG 2 cut(s) 359, 1274
CciI TCATGA 1 cut(s) 1180
CfoI GCGC 2 cut(s) 572, 1241
Cfr10I RCCGGY 2 cut(s) 289, 790
ClaI ATCGAT 1 cut(s) 867
Csp6I GTAC 3 cut(s) 469, 590, 1165
CviAII CATG 2 cut(s) 1059, 1181
CviQI GTAC 3 cut(s) 469, 590, 1165
DrdI GACNNNNNNGTC 1 cut(s) 11
DseDI GACNNNNNNGTC 1 cut(s) 11
EaeI YGGCCR 1 cut(s) 287
EagI CGGCCG 1 cut(s) 287
Eam1104I CTCTTC 6 cut(s) 61, 222, 313, 510, 954, 1030
EarI CTCTTC 6 cut(s) 61, 222, 313, 510, 954, 1030
EciI GGCGGA 2 cut(s) 124, 1032
EclXI CGGCCG 1 cut(s) 287
Eco130I CCWWGG 1 cut(s) 1058
Eco32I GATATC 1 cut(s) 333
Eco52I CGGCCG 1 cut(s) 287
Eco57I CTGAAG 1 cut(s) 1143
Eco88I CYCGRG 1 cut(s) 1249
EcoRI GAATTC 3 cut(s) 337, 480, 809
EcoRII CCWGG 1 cut(s) 164
EcoRV GATATC 1 cut(s) 333
EcoT14I CCWWGG 1 cut(s) 1058
EcoT22I ATGCAT 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 1058
Esp3I CGTCTC 2 cut(s) 121, 434
FaeI CATG 2 cut(s) 1062, 1184
FaiI YATR 4 cut(s) 394, 584, 1060, 1182
FalI AAGNNNNNCTT 4 cut(s) 519, 551, 987, 1019
FaqI GGGAC 1 cut(s) 768
FatI CATG 2 cut(s) 1058, 1180
FokI GGATG 2 cut(s) 185, 904
FspBI CTAG 2 cut(s) 828, 1091
GlaI GCGC 2 cut(s) 571, 1240
HaeIII GGCC 1 cut(s) 289
HapII CCGG 5 cut(s) 24, 225, 290, 519, 791
HhaI GCGC 2 cut(s) 572, 1241
Hin1II CATG 2 cut(s) 1062, 1184
Hin6I GCGC 2 cut(s) 570, 1239
HinP1I GCGC 2 cut(s) 570, 1239
HincII GTYRAC 3 cut(s) 646, 726, 1225
HindII GTYRAC 3 cut(s) 646, 726, 1225
HindIII AAGCTT 1 cut(s) 803
HpaI GTTAAC 1 cut(s) 1225
HpaII CCGG 5 cut(s) 24, 225, 290, 519, 791
HphI GGTGA 2 cut(s) 24, 260
Hpy166II GTNNAC 7 cut(s) 578, 592, 646, 726, 941, 1225, 1297
Hpy8I GTNNAC 7 cut(s) 578, 592, 646, 726, 941, 1225, 1297
Hpy99I CGWCG 2 cut(s) 464, 683
HpyAV CCTTC 3 cut(s) 30, 45, 519
HpyCH4III ACNGT 3 cut(s) 248, 1261, 1276
HpyCH4IV ACGT 4 cut(s) 190, 252, 943, 1079
HpyCH4V TGCA 7 cut(s) 160, 275, 348, 855, 1139, 1151, 1268
HpyF10VI GCNNNNNNNGC 8 cut(s) 107, 548, 703, 747, 833, 1014, 1148, 1205
HpySE526I ACGT 4 cut(s) 190, 252, 943, 1079
Hsp92II CATG 2 cut(s) 1062, 1184
HspAI GCGC 2 cut(s) 570, 1239
Kpn2I TCCGGA 1 cut(s) 518
KroI GCCGGC 2 cut(s) 289, 790
KroNI GCCGGC 2 cut(s) 291, 792
KspAI GTTAAC 1 cut(s) 1225
LmnI GCTCC 1 cut(s) 1063
Lsp1109I GCAGC 5 cut(s) 97, 304, 709, 1151, 1252
LweI GCATC 4 cut(s) 147, 207, 750, 1126
MaeI CTAG 2 cut(s) 828, 1091
MaeII ACGT 4 cut(s) 190, 252, 943, 1079
MaeIII GTNAC 5 cut(s) 85, 248, 253, 300, 453
MflI RGATCY 4 cut(s) 151, 368, 601, 777
MluCI AATT 3 cut(s) 337, 480, 809
MlyI GAGTC 1 cut(s) 693
MmeI TCCRAC 2 cut(s) 442, 661
Mph1103I ATGCAT 1 cut(s) 162
MroI TCCGGA 1 cut(s) 518
MroNI GCCGGC 2 cut(s) 289, 790
MroXI GAANNNNTTC 1 cut(s) 484
MseI TTAA 6 cut(s) 561, 720, 1082, 1131, 1224, 1278
MslI CAYNNNNRTG 2 cut(s) 1057, 1342
MspA1I CMGCKG 3 cut(s) 110, 143, 554
MspI CCGG 5 cut(s) 24, 225, 290, 519, 791
MspR9I CCNGG 1 cut(s) 166
Mva1269I GAATGC 1 cut(s) 160
MvaI CCWGG 1 cut(s) 166
MvnI CGCG 3 cut(s) 570, 655, 716
MwoI GCNNNNNNNGC 8 cut(s) 107, 548, 703, 747, 833, 1014, 1148, 1205
NaeI GCCGGC 2 cut(s) 291, 792
NcoI CCATGG 1 cut(s) 1058
NgoMIV GCCGGC 2 cut(s) 289, 790
NlaIII CATG 2 cut(s) 1062, 1184
NmuCI GTSAC 1 cut(s) 248
NsiI ATGCAT 1 cut(s) 162
NspV TTCGAA 1 cut(s) 525
PagI TCATGA 1 cut(s) 1180
PcsI WCGNNNNNNNCGW 2 cut(s) 449, 873
PctI GAATGC 1 cut(s) 160
PdiI GCCGGC 2 cut(s) 291, 792
PdmI GAANNNNTTC 1 cut(s) 484
PflFI GACNNNGTC 2 cut(s) 884, 945
PflMI CCANNNNNTGG 1 cut(s) 359
Ple19I CGATCG 1 cut(s) 286
PleI GAGTC 1 cut(s) 692
PpsI GAGTC 1 cut(s) 692
Psp1406I AACGTT 1 cut(s) 190
Psp6I CCWGG 1 cut(s) 164
PspGI CCWGG 1 cut(s) 164
PstI CTGCAG 1 cut(s) 1270
PstNI CAGNNNCTG 2 cut(s) 359, 1274
PsuI RGATCY 4 cut(s) 151, 368, 601, 777
PsyI GACNNNGTC 2 cut(s) 884, 945
PvuI CGATCG 1 cut(s) 286
RsaI GTAC 3 cut(s) 470, 591, 1166
RsaNI GTAC 3 cut(s) 469, 590, 1165
RseI CAYNNNNRTG 2 cut(s) 1057, 1342
SaqAI TTAA 6 cut(s) 561, 720, 1082, 1131, 1224, 1278
SchI GAGTC 1 cut(s) 693
ScrFI CCNGG 1 cut(s) 166
SfaNI GCATC 4 cut(s) 147, 207, 750, 1126
SfcI CTRYAG 1 cut(s) 1266
SfuI TTCGAA 1 cut(s) 525
SmiMI CAYNNNNRTG 2 cut(s) 1057, 1342
SmlI CTYRAG 2 cut(s) 701, 950
SmoI CTYRAG 2 cut(s) 701, 950
Sse9I AATT 3 cut(s) 337, 480, 809
SspMI CTAG 2 cut(s) 828, 1091
StyD4I CCNGG 1 cut(s) 164
StyI CCWWGG 1 cut(s) 1058
TaaI ACNGT 3 cut(s) 248, 1261, 1276
TaiI ACGT 4 cut(s) 193, 255, 946, 1082
TaqI TCGA 8 cut(s) 148, 335, 478, 497, 525, 867, 957, 1289
TasI AATT 3 cut(s) 337, 480, 809
TatI WGTACW 1 cut(s) 468
TauI GCSGC 5 cut(s) 30, 110, 140, 143, 554
Tru1I TTAA 6 cut(s) 561, 720, 1082, 1131, 1224, 1278
Tru9I TTAA 6 cut(s) 561, 720, 1082, 1131, 1224, 1278
TscAI CASTG 1 cut(s) 943
TseFI GTSAC 1 cut(s) 248
TseI GCWGC 5 cut(s) 110, 317, 697, 1139, 1265
Tsp45I GTSAC 1 cut(s) 248
TspDTI ATGAA 4 cut(s) 201, 473, 885, 906
TspGWI ACGGA 4 cut(s) 30, 73, 441, 667
TspRI CASTG 1 cut(s) 943
Tth111I GACNNNGTC 2 cut(s) 884, 945
Van91I CCANNNNNTGG 1 cut(s) 359
XapI RAATTY 3 cut(s) 337, 480, 809
XmnI GAANNNNTTC 1 cut(s) 484
XspI CTAG 2 cut(s) 828, 1091
Zsp2I ATGCAT 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.