Rroxscaffold_2G00131980

Hyccin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014272
Physical Location & Seq
Reverse (-)
68799285 .. 68801380
2096 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00131980.1

Sequence Viewer

Length: 1194 bp
ATGGTGGGAGTCCATCTCCAAAGCCCGCTCCCGCATCCACTCCCTCGCCACTCTCCTCGACCCTTCTCTCTCCGCCCCCTCTCCTCCCTAGCCGACTCCGACCGCCCCGCCCTCTCTCTCCTCTCCTCCCTCGAAGCCTACGCCGCCGTTTCCGCCGTTCTCTCCGCCCCCCTCTCCGGCTCCGGATCCGACCCGCTCTGCAACTGGCTCTACGACACCTTCCTCTCCTCCGACCCCCATCTACGACTCGTCGTTTTCTCCTTCCTCCCTGTCCTCGCCGGCCTCTACCTCTCCCGCATCCACTCTCTCTCCTCTGACTCTCTTACCTCACTTCCCTCCCTCGCCGGCTTTGAGGCCGTGCTCCTCGCTCTCTACGCCGCCGAGACCAAGGCCCGAAACGGCAAGCCCATTGTCGTTTCGATCCCGGACCTATCTCAGCCCTCTCTCTACCACATTCCTCGAAGCCAGAAGACGACTAATCCCAACTCCAATTCCAGTAATATCCAATCCGTTGGGGTTTTGTCGCCGCCGCTGGAGCCCCAGGTCGCCGTCAAGTCAACCAAACGGGCCTGCATTGTTGGAGTTGCGCTAGATTCTTATTACAAGCACATTTCGCAGATGCCCATTTGGTCCAAGATAGATTTTTGCAGGTTCTTAGCTTCTTGGGCCGGCCCGGATTGCTCTTGTCAGCGGCAATTCGAATCCGGTGATAACCACAGTGAGCCAGAGATTGCTGGGTTCTTGGAGGCCGGCAGTGGGAACGGGAGTGGTAATGAGAATGAGAGTGAGATTGAGATTGAGGGTGATGTGGCGGAAGAAATGGGCCAACTGAGAATTGAGCAAAATGGGACCAGCAATGGGGTCAGTCATGTGGAGGACTCAAACGGGGCGAAGATTCCATTGCCGTGGGAGCTTTTGCAGCCGGCGTTGCGAATATTGGGGCATTGTTTGTTGGCGCCATTGAATTCTCAGGAGGTTAAGGACGCTGCTTCCATTGCAGTGAGGAGATTGTATGCCAGGGCATCTCATGATTTGGTGCCGCAGGCAATTTTAGCTACTCAGAGTCTCATTCAGCTTGATAACAGGGGGCGTGAGGCAGCCAAGGCTGCAGTGGTGGTTGCTGCTAATTCATCTTCGAATGTTAACACGCCAAGCAAAGCAAAGAAACCTGAAATCCTTTTGGTCTCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hyccin

Protein Analysis

397

Amino Acids

42.47

Weight (kDa)

6.13

Isoelectric Point (pI)

57.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hyccin PF09790 32 - 358 7.6e-60 Hyccin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64090
fragaria_vesca FvH4_1g19990
malus_domestica MD05G1042400.v1.1 MD10G1049000.v1.1
prunus_persica Prupe.8G059200_v2.0.a1
pyrus_communis pycom05g03500
rosa_chinensis RchiOBHm_Chr2g0111931
rosa_laevigata RLG00000017939 RLG00000034552
rosa_multiflora Rmu_sc0005679.1_g000010
rosa_roxburghii Rroxscaffold_2G00131980
rosa_rugosa Rorug02G0177300
rosa_samantha Rh2AG229700 Rh2BG242400 Rh2CG233800 Rh2DG237400
rosa_wichuraiana Rw2G017770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 639
AccB1I GGYRCC 2 cut(s) 955, 1036
AccBSI CCGCTC 2 cut(s) 28, 196
AccIII TCCGGA 1 cut(s) 182
AclWI GGATC 3 cut(s) 180, 193, 415
AcsI RAATTY 1 cut(s) 964
AcyI GRCGYC 1 cut(s) 956
AfiI CCNNNNNNNGG 3 cut(s) 176, 756, 858
AgsI TTSAA 1 cut(s) 964
AjnI CCWGG 2 cut(s) 540, 1016
AluBI AGCT 4 cut(s) 659, 913, 1055, 1075
AluI AGCT 4 cut(s) 659, 913, 1055, 1075
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 3 cut(s) 377, 1070, 1189
AlwI GGATC 3 cut(s) 180, 193, 415
Aor13HI TCCGGA 1 cut(s) 182
AoxI GGCC 8 cut(s) 280, 354, 390, 567, 666, 670, 747, 823
ApeKI GCWGC 5 cut(s) 919, 986, 1097, 1106, 1121
ApoI RAATTY 1 cut(s) 964
AspLEI GCGC 2 cut(s) 589, 958
AspS9I GGNCC 8 cut(s) 391, 427, 567, 630, 666, 671, 823, 849
AsuC2I CCSGG 2 cut(s) 425, 674
AsuHPI GGTGA 2 cut(s) 719, 815
AsuII TTCGAA 2 cut(s) 699, 1136
AvaII GGWCC 3 cut(s) 427, 630, 849
BamHI GGATCC 1 cut(s) 185
BanI GGYRCC 2 cut(s) 955, 1036
BanII GRGCYC 1 cut(s) 540
BbsI GAAGAC 1 cut(s) 476
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 5 cut(s) 931, 973, 1093, 1108, 1109
BccI CCATC 2 cut(s) 21, 246
BceAI ACGGC 6 cut(s) 131, 140, 341, 415, 533, 889
BciT130I CCWGG 2 cut(s) 542, 1018
BcnI CCSGG 2 cut(s) 425, 674
BcoDI GTCTC 3 cut(s) 377, 1070, 1189
BfaI CTAG 2 cut(s) 89, 590
BfmI CTRYAG 1 cut(s) 1107
BfoI RGCGCY 1 cut(s) 959
BfuAI ACCTGC 1 cut(s) 639
Bme1390I CCNGG 4 cut(s) 425, 542, 674, 1018
Bme18I GGWCC 3 cut(s) 427, 630, 849
BmgT120I GGNCC 8 cut(s) 391, 427, 567, 630, 666, 671, 823, 849
BmiI GGNNCC 6 cut(s) 181, 187, 537, 850, 957, 1038
BmrFI CCNGG 4 cut(s) 425, 542, 674, 1018
BmsI GCATC 4 cut(s) 43, 306, 609, 1031
BpiI GAAGAC 1 cut(s) 476
BplI GAGNNNNNCTC 1 cut(s) 32
BpmI CTGGAG 1 cut(s) 554
Bpu14I TTCGAA 2 cut(s) 699, 1136
BpuMI CCSGG 2 cut(s) 425, 674
BsaHI GRCGYC 1 cut(s) 956
BsaI GGTCTC 2 cut(s) 377, 1189
BsaJI CCNNGG 5 cut(s) 387, 540, 905, 1017, 1101
BsaWI WCCGGW 2 cut(s) 182, 704
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 3 cut(s) 176, 756, 858
Bse118I RCCGGY 5 cut(s) 278, 344, 668, 749, 922
Bse1I ACTGG 2 cut(s) 209, 495
Bse3DI GCAATG 3 cut(s) 862, 899, 993
BseAI TCCGGA 1 cut(s) 182
BseBI CCWGG 2 cut(s) 542, 1018
BseDI CCNNGG 5 cut(s) 387, 540, 905, 1017, 1101
BseGI GGATG 2 cut(s) 34, 297
BseLI CCNNNNNNNGG 3 cut(s) 176, 756, 858
BseMI GCAATG 3 cut(s) 862, 899, 993
BseMII CTCAG 4 cut(s) 449, 821, 983, 1073
BseNI ACTGG 2 cut(s) 209, 495
BseRI GAGGAG 8 cut(s) 45, 73, 110, 115, 217, 301, 353, 1018
BseXI GCAGC 5 cut(s) 931, 973, 1093, 1108, 1109
BseYI CCCAGC 1 cut(s) 734
Bsh1285I CGRYCG 1 cut(s) 103
BshFI GGCC 8 cut(s) 282, 356, 392, 569, 668, 672, 749, 825
BshNI GGYRCC 2 cut(s) 955, 1036
BsiEI CGRYCG 1 cut(s) 103
BsiHKAI GWGCWC 1 cut(s) 363
BslFI GGGAC 1 cut(s) 862
BslI CCNNNNNNNGG 3 cut(s) 176, 756, 858
BsmAI GTCTC 3 cut(s) 377, 1070, 1189
BsmFI GGGAC 1 cut(s) 862
BsnI GGCC 8 cut(s) 282, 356, 392, 569, 668, 672, 749, 825
Bso31I GGTCTC 2 cut(s) 377, 1189
Bsp119I TTCGAA 2 cut(s) 699, 1136
Bsp1286I GDGCHC 2 cut(s) 363, 540
Bsp13I TCCGGA 1 cut(s) 182
Bsp143I GATC 2 cut(s) 185, 420
BspANI GGCC 8 cut(s) 282, 356, 392, 569, 668, 672, 749, 825
BspCNI CTCAG 4 cut(s) 448, 822, 982, 1072
BspEI TCCGGA 1 cut(s) 182
BspHI TCATGA 1 cut(s) 1027
BspLI GGNNCC 6 cut(s) 181, 187, 537, 850, 957, 1038
BspMAI CTGCAG 1 cut(s) 1111
BspMI ACCTGC 1 cut(s) 639
BspPI GGATC 3 cut(s) 180, 193, 415
BspT104I TTCGAA 2 cut(s) 699, 1136
BspT107I GGYRCC 2 cut(s) 955, 1036
BspTNI GGTCTC 2 cut(s) 377, 1189
BsrBI CCGCTC 2 cut(s) 28, 196
BsrDI GCAATG 3 cut(s) 862, 899, 993
BsrFI RCCGGY 5 cut(s) 278, 344, 668, 749, 922
BsrI ACTGG 2 cut(s) 209, 495
BssAI RCCGGY 5 cut(s) 278, 344, 668, 749, 922
BssECI CCNNGG 5 cut(s) 387, 540, 905, 1017, 1101
BssMI GATC 2 cut(s) 185, 420
BssNI GRCGYC 1 cut(s) 956
BssT1I CCWWGG 2 cut(s) 387, 1101
Bst2UI CCWGG 2 cut(s) 542, 1018
Bst4CI ACNGT 1 cut(s) 719
BstACI GRCGYC 1 cut(s) 956
BstBI TTCGAA 2 cut(s) 699, 1136
BstC8I GCNNGC 9 cut(s) 26, 280, 346, 404, 571, 670, 751, 924, 1044
BstDEI CTNAG 5 cut(s) 435, 655, 830, 969, 1059
BstDSI CCRYGG 1 cut(s) 905
BstF5I GGATG 2 cut(s) 34, 297
BstH2I RGCGCY 1 cut(s) 959
BstHHI GCGC 2 cut(s) 589, 958
BstKTI GATC 2 cut(s) 188, 423
BstMAI GTCTC 3 cut(s) 377, 1070, 1189
BstMBI GATC 2 cut(s) 185, 420
BstMCI CGRYCG 1 cut(s) 103
BstNI CCWGG 2 cut(s) 542, 1018
BstSCI CCNGG 4 cut(s) 423, 540, 672, 1016
BstSFI CTRYAG 1 cut(s) 1107
BstV1I GCAGC 5 cut(s) 931, 973, 1093, 1108, 1109
BstV2I GAAGAC 1 cut(s) 476
BstX2I RGATCY 1 cut(s) 185
BstXI CCANNNNNNTGG 2 cut(s) 512, 906
BstYI RGATCY 1 cut(s) 185
BsuRI GGCC 8 cut(s) 282, 356, 392, 569, 668, 672, 749, 825
BtgI CCRYGG 1 cut(s) 905
BtsCI GGATG 2 cut(s) 34, 297
BtsI GCAGTG 3 cut(s) 760, 1005, 1116
BtsIMutI CAGTG 4 cut(s) 724, 760, 1005, 1116
BveI ACCTGC 1 cut(s) 639
Cac8I GCNNGC 9 cut(s) 26, 280, 346, 404, 571, 670, 751, 924, 1044
CciI TCATGA 1 cut(s) 1027
CfoI GCGC 2 cut(s) 589, 958
Cfr10I RCCGGY 5 cut(s) 278, 344, 668, 749, 922
Cfr13I GGNCC 8 cut(s) 391, 427, 567, 630, 666, 671, 823, 849
CseI GACGC 1 cut(s) 992
CviAII CATG 2 cut(s) 869, 1028
DdeI CTNAG 5 cut(s) 435, 655, 830, 969, 1059
DinI GGCGCC 1 cut(s) 957
DpnI GATC 2 cut(s) 187, 422
DpnII GATC 2 cut(s) 185, 420
EciI GGCGGA 4 cut(s) 62, 142, 154, 827
Eco130I CCWWGG 2 cut(s) 387, 1101
Eco24I GRGCYC 1 cut(s) 540
Eco31I GGTCTC 2 cut(s) 377, 1189
Eco47I GGWCC 3 cut(s) 427, 630, 849
EcoRI GAATTC 1 cut(s) 964
EcoRII CCWGG 2 cut(s) 540, 1016
EcoT14I CCWWGG 2 cut(s) 387, 1101
EcoT38I GRGCYC 1 cut(s) 540
EgeI GGCGCC 1 cut(s) 957
EheI GGCGCC 1 cut(s) 957
ErhI CCWWGG 2 cut(s) 387, 1101
FaeI CATG 2 cut(s) 872, 1031
FaiI YATR 3 cut(s) 870, 1014, 1029
FaqI GGGAC 1 cut(s) 862
FatI CATG 2 cut(s) 868, 1027
FauI CCCGC 5 cut(s) 33, 39, 115, 201, 302
FokI GGATG 2 cut(s) 21, 284
FriOI GRGCYC 1 cut(s) 540
FseI GGCCGGCC 1 cut(s) 672
FspBI CTAG 2 cut(s) 89, 590
GlaI GCGC 2 cut(s) 588, 957
GsaI CCCAGC 1 cut(s) 738
GsuI CTGGAG 1 cut(s) 554
HaeII RGCGCY 1 cut(s) 959
HaeIII GGCC 8 cut(s) 282, 356, 392, 569, 668, 672, 749, 825
HgaI GACGC 1 cut(s) 992
HhaI GCGC 2 cut(s) 589, 958
Hin1I GRCGYC 1 cut(s) 956
Hin1II CATG 2 cut(s) 872, 1031
Hin6I GCGC 2 cut(s) 587, 956
HinP1I GCGC 2 cut(s) 587, 956
HincII GTYRAC 2 cut(s) 558, 1144
HindII GTYRAC 2 cut(s) 558, 1144
HinfI GANTC 9 cut(s) 9, 95, 246, 317, 593, 701, 878, 895, 1063
HpaI GTTAAC 1 cut(s) 1144
HphI GGTGA 2 cut(s) 719, 815
Hpy166II GTNNAC 2 cut(s) 558, 1144
Hpy188I TCNGA 5 cut(s) 100, 190, 232, 316, 1062
Hpy188III TCNNGA 3 cut(s) 183, 971, 1028
Hpy8I GTNNAC 2 cut(s) 558, 1144
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 3 cut(s) 73, 229, 271
HpyCH4III ACNGT 1 cut(s) 719
HpyCH4V TGCA 6 cut(s) 201, 573, 648, 919, 998, 1109
HpyF3I CTNAG 5 cut(s) 435, 655, 830, 969, 1059
Hsp92I GRCGYC 1 cut(s) 956
Hsp92II CATG 2 cut(s) 872, 1031
HspAI GCGC 2 cut(s) 587, 956
KasI GGCGCC 1 cut(s) 955
Kpn2I TCCGGA 1 cut(s) 182
KroI GCCGGC 5 cut(s) 278, 344, 668, 749, 922
KroNI GCCGGC 5 cut(s) 280, 346, 670, 751, 924
KspAI GTTAAC 1 cut(s) 1144
Kzo9I GATC 2 cut(s) 185, 420
LmnI GCTCC 5 cut(s) 33, 185, 366, 535, 910
Lsp1109I GCAGC 5 cut(s) 931, 973, 1093, 1108, 1109
LweI GCATC 4 cut(s) 43, 306, 609, 1031
MaeI CTAG 2 cut(s) 89, 590
MalI GATC 2 cut(s) 187, 422
MbiI CCGCTC 2 cut(s) 28, 196
MboI GATC 2 cut(s) 185, 420
MboII GAAGA 4 cut(s) 481, 827, 904, 1125
MflI RGATCY 1 cut(s) 185
MhlI GDGCHC 2 cut(s) 363, 540
MluCI AATT 6 cut(s) 490, 695, 834, 964, 1047, 1126
Mly113I GGCGCC 1 cut(s) 956
MlyI GAGTC 6 cut(s) 18, 89, 240, 311, 872, 1072
MmeI TCCRAC 4 cut(s) 123, 213, 255, 559
MroI TCCGGA 1 cut(s) 182
MroNI GCCGGC 5 cut(s) 278, 344, 668, 749, 922
MseI TTAA 2 cut(s) 978, 1143
MslI CAYNNNNRTG 2 cut(s) 904, 998
MspA1I CMGCKG 2 cut(s) 532, 691
MspR9I CCNGG 4 cut(s) 425, 542, 674, 1018
MvaI CCWGG 2 cut(s) 542, 1018
NaeI GCCGGC 5 cut(s) 280, 346, 670, 751, 924
NarI GGCGCC 1 cut(s) 956
NciI CCSGG 2 cut(s) 425, 674
NdeII GATC 2 cut(s) 185, 420
NgoMIV GCCGGC 5 cut(s) 278, 344, 668, 749, 922
NlaIII CATG 2 cut(s) 872, 1031
NlaIV GGNNCC 6 cut(s) 181, 187, 537, 850, 957, 1038
NmeAIII GCCGAG 1 cut(s) 406
NspV TTCGAA 2 cut(s) 699, 1136
PagI TCATGA 1 cut(s) 1027
PdiI GCCGGC 5 cut(s) 280, 346, 670, 751, 924
PfeI GAWTC 3 cut(s) 593, 701, 895
PfoI TCCNGGA 1 cut(s) 423
PleI GAGTC 6 cut(s) 17, 89, 240, 311, 872, 1071
PluTI GGCGCC 1 cut(s) 959
PpsI GAGTC 6 cut(s) 17, 89, 240, 311, 872, 1071
Psp6I CCWGG 2 cut(s) 540, 1016
PspFI CCCAGC 1 cut(s) 734
PspGI CCWGG 2 cut(s) 540, 1016
PspN4I GGNNCC 6 cut(s) 181, 187, 537, 850, 957, 1038
PspPI GGNCC 8 cut(s) 391, 427, 567, 630, 666, 671, 823, 849
PstI CTGCAG 1 cut(s) 1111
PsuI RGATCY 1 cut(s) 185
RigI GGCCGGCC 1 cut(s) 672
RseI CAYNNNNRTG 2 cut(s) 904, 998
SaqAI TTAA 2 cut(s) 978, 1143
Sau3AI GATC 2 cut(s) 185, 420
Sau96I GGNCC 8 cut(s) 391, 427, 567, 630, 666, 671, 823, 849
SchI GAGTC 6 cut(s) 18, 89, 240, 311, 872, 1072
ScrFI CCNGG 4 cut(s) 425, 542, 674, 1018
SduI GDGCHC 2 cut(s) 363, 540
SfaNI GCATC 4 cut(s) 43, 306, 609, 1031
SfcI CTRYAG 1 cut(s) 1107
SfoI GGCGCC 1 cut(s) 957
SfuI TTCGAA 2 cut(s) 699, 1136
SinI GGWCC 3 cut(s) 427, 630, 849
SmiMI CAYNNNNRTG 2 cut(s) 904, 998
Sse9I AATT 6 cut(s) 490, 695, 834, 964, 1047, 1126
SspDI GGCGCC 1 cut(s) 955
SspI AATATT 1 cut(s) 936
SspMI CTAG 2 cut(s) 89, 590
StyD4I CCNGG 4 cut(s) 423, 540, 672, 1016
StyI CCWWGG 2 cut(s) 387, 1101
TaaI ACNGT 1 cut(s) 719
TaqI TCGA 6 cut(s) 58, 132, 419, 460, 699, 1136
TasI AATT 6 cut(s) 490, 695, 834, 964, 1047, 1126
TauI GCSGC 6 cut(s) 146, 380, 529, 532, 694, 1042
TfiI GAWTC 3 cut(s) 593, 701, 895
Tru1I TTAA 2 cut(s) 978, 1143
Tru9I TTAA 2 cut(s) 978, 1143
TscAI CASTG 4 cut(s) 724, 760, 1005, 1116
TseI GCWGC 5 cut(s) 919, 986, 1097, 1106, 1121
TspDTI ATGAA 1 cut(s) 1119
TspGWI ACGGA 1 cut(s) 499
TspRI CASTG 4 cut(s) 724, 760, 1005, 1116
VpaK11BI GGWCC 3 cut(s) 427, 630, 849
XapI RAATTY 1 cut(s) 964
XcmI CCANNNNNNNNNTGG 1 cut(s) 1108
XspI CTAG 2 cut(s) 89, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.