RLG00000017939

Hyccin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014272
Physical Location & Seq
Forward (+)
23274814 .. 23276750
1937 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017939

Sequence Viewer

Length: 1341 bp
ATGGACCTCTACCCAAATCACAGCACCAACTCCCCCTCCTCCTCCGCCTCCTCCACCGCCACCAACCCAAACCCAAGCGCAAACCCCAGACCCACCCGCAACACCATCTCCTCCCTCACCGCCACCGACGACGACCCAATGCACTCCTGGTGGGAGTCCATCTCCAAAGCCCGCTCCCGCATCCACTCCCTCGCCACTCTCCTCGACCCTTCTCTCTCCGCCCCCCTCTCCTCCCTCGCCGACTCCGACCGCCCCGCCCTCTCTCTCCTCTCCTCCCTCGAAGCCTACGCCGCCGTTTCCGCCGTCCTCTCCGCCCCTCTCTCCGGCTCCGGATCCGACCCGCTCTGCAACTGGCTCTACGACACCTTCCTCTCCTCCGACCCCCATCTACGACTCGTCGTTTTCTCCTTCCTCCCTCTCCTCGCCGGCCTCTACCTCTCCCGCATCCACTCTCTCTCCTCTGACTCTCTTACCTCGCTTCCCTCCCTCGCCGGCTTTGAGGCCGTGCTCCTCGCTCTCTACGCCGCCGAGACCAAGGCCCGAAACGGCAAGCCCATTGTCGTTTCGATCCCGGACCTATCTCAGCCCTCTCTCTACCACATTCCTCGAAGCCAGAAGACGACTAATCCCAACTCCAATTCCAGTAATATCCAATCCGTTGGGGTTTTGTCGCCGCCGCTTGAGCCCCAGGTTGCCGTCAAGTCAACCAAACGGGCCTGCATTGTCGGAGTTGCGCTGGATTCTTATTACAAGCACATTTCACAGATGCCCACTTGGTCCAAGATGGATTTTTGCAGGTTCCTAGCTTCTTGGGCAGGCCCGGATTGCTCTTGTCAGCGGCAATTCGAATCCGGTGATAACCACAGTGAGCCGGAGATTGCTGGGTTCTTGGAGGCCGGCAATGGGAATGGGAGTGGTAATGAGAGTGAGAGTGAGATTGAGATTGAGGGTGATGTGGCGGAAGAAATGGGTCAACTGAGAATTGAGCAAAATGGAACCAACAATGGCATCAGTCATGTGGAGGACTCAAAGGGGGCGAAGATTCCATTGCCGTGGGAGCTTTTGCAGCCGGCGCTGCGAATATTGGGGCATTGTTTGTTGGCACCGTTGAATTCTCAGGAGGTTAAGGACGCTGCTTCCATTGCGGTGAGGAGATTGTATGCCAGGGCATCTCATGATTTGGTGCCGCAGGCGATTTTAGCTACTCAGAGTCTCATTCAGCTTGATAACAGGGGGCGTGAGGCAGTCAAGGCTGCAGTGGCGGCTGCTGCTAATTCATCTTCGAATGTTAACACGCCAAGCAAAGCTAAGAAACCTGAAATCCTTTTGGTCTCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hyccin

Protein Analysis

447

Amino Acids

47.61

Weight (kDa)

5.79

Isoelectric Point (pI)

53.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hyccin PF09790 81 - 406 3.7e-60 Hyccin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64090
fragaria_vesca FvH4_1g19990
malus_domestica MD05G1042400.v1.1 MD10G1049000.v1.1
prunus_persica Prupe.8G059200_v2.0.a1
pyrus_communis pycom05g03500
rosa_chinensis RchiOBHm_Chr2g0111931
rosa_laevigata RLG00000017939 RLG00000034552
rosa_multiflora Rmu_sc0005679.1_g000010
rosa_roxburghii Rroxscaffold_2G00131980
rosa_rugosa Rorug02G0177300
rosa_samantha Rh2AG229700 Rh2BG242400 Rh2CG233800 Rh2DG237400
rosa_wichuraiana Rw2G017770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 786
AccB1I GGYRCC 2 cut(s) 1102, 1183
AccBSI CCGCTC 2 cut(s) 174, 343
AccIII TCCGGA 1 cut(s) 329
AclWI GGATC 3 cut(s) 327, 340, 562
AcsI RAATTY 1 cut(s) 1111
AfiI CCNNNNNNNGG 2 cut(s) 323, 903
AgsI TTSAA 1 cut(s) 1111
AjnI CCWGG 3 cut(s) 146, 687, 1163
AluBI AGCT 5 cut(s) 806, 1060, 1202, 1222, 1307
AluI AGCT 5 cut(s) 806, 1060, 1202, 1222, 1307
Alw21I GWGCWC 1 cut(s) 510
Alw26I GTCTC 3 cut(s) 524, 1217, 1336
AlwI GGATC 3 cut(s) 327, 340, 562
Aor13HI TCCGGA 1 cut(s) 329
AoxI GGCC 6 cut(s) 427, 501, 537, 714, 817, 894
ApeKI GCWGC 6 cut(s) 1066, 1075, 1133, 1253, 1265, 1268
ApoI RAATTY 1 cut(s) 1111
AspLEI GCGC 3 cut(s) 80, 736, 1075
AspS9I GGNCC 6 cut(s) 4, 538, 574, 714, 777, 818
AsuC2I CCSGG 2 cut(s) 572, 821
AsuHPI GGTGA 4 cut(s) 109, 866, 962, 1159
AsuII TTCGAA 2 cut(s) 846, 1283
AvaII GGWCC 3 cut(s) 4, 574, 777
BamHI GGATCC 1 cut(s) 332
BanI GGYRCC 2 cut(s) 1102, 1183
BanII GRGCYC 1 cut(s) 687
BbsI GAAGAC 1 cut(s) 623
Bbv12I GWGCWC 1 cut(s) 510
BbvI GCAGC 6 cut(s) 1062, 1078, 1120, 1240, 1252, 1255
BccI CCATC 4 cut(s) 113, 167, 393, 778
BceAI ACGGC 6 cut(s) 278, 287, 488, 562, 680, 1036
BciT130I CCWGG 3 cut(s) 148, 689, 1165
BcnI CCSGG 2 cut(s) 572, 821
BcoDI GTCTC 3 cut(s) 524, 1217, 1336
BfaI CTAG 1 cut(s) 803
BfmI CTRYAG 1 cut(s) 1254
BfoI RGCGCY 1 cut(s) 1076
BfuAI ACCTGC 1 cut(s) 786
Bme1390I CCNGG 5 cut(s) 148, 572, 689, 821, 1165
Bme18I GGWCC 3 cut(s) 4, 574, 777
BmgT120I GGNCC 6 cut(s) 4, 538, 574, 714, 777, 818
BmiI GGNNCC 6 cut(s) 328, 334, 800, 997, 1104, 1185
BmrFI CCNGG 5 cut(s) 148, 572, 689, 821, 1165
BmsI GCATC 5 cut(s) 189, 453, 756, 1017, 1178
BpiI GAAGAC 1 cut(s) 623
BplI GAGNNNNNCTC 2 cut(s) 146, 178
Bpu14I TTCGAA 2 cut(s) 846, 1283
BpuEI CTTGAG 1 cut(s) 701
BpuMI CCSGG 2 cut(s) 572, 821
BsaI GGTCTC 2 cut(s) 524, 1336
BsaJI CCNNGG 4 cut(s) 534, 687, 1052, 1164
BsaWI WCCGGW 2 cut(s) 329, 851
BsaXI ACNNNNNCTCC 6 cut(s) 20, 50, 92, 122, 440, 470
Bsc4I CCNNNNNNNGG 2 cut(s) 323, 903
Bse118I RCCGGY 4 cut(s) 425, 491, 896, 1069
Bse1I ACTGG 2 cut(s) 356, 642
Bse3DI GCAATG 3 cut(s) 907, 1046, 1140
BseAI TCCGGA 1 cut(s) 329
BseBI CCWGG 3 cut(s) 148, 689, 1165
BseDI CCNNGG 4 cut(s) 534, 687, 1052, 1164
BseGI GGATG 2 cut(s) 180, 444
BseLI CCNNNNNNNGG 2 cut(s) 323, 903
BseMI GCAATG 3 cut(s) 907, 1046, 1140
BseMII CTCAG 4 cut(s) 596, 968, 1130, 1220
BseNI ACTGG 2 cut(s) 356, 642
BseXI GCAGC 6 cut(s) 1062, 1078, 1120, 1240, 1252, 1255
BseYI CCCAGC 1 cut(s) 881
Bsh1285I CGRYCG 1 cut(s) 250
BshFI GGCC 6 cut(s) 429, 503, 539, 716, 819, 896
BshNI GGYRCC 2 cut(s) 1102, 1183
BsiEI CGRYCG 1 cut(s) 250
BsiHKAI GWGCWC 1 cut(s) 510
BslI CCNNNNNNNGG 2 cut(s) 323, 903
BsmAI GTCTC 3 cut(s) 524, 1217, 1336
BsnI GGCC 6 cut(s) 429, 503, 539, 716, 819, 896
Bso31I GGTCTC 2 cut(s) 524, 1336
Bsp119I TTCGAA 2 cut(s) 846, 1283
Bsp1286I GDGCHC 2 cut(s) 510, 687
Bsp13I TCCGGA 1 cut(s) 329
Bsp143I GATC 2 cut(s) 332, 567
BspANI GGCC 6 cut(s) 429, 503, 539, 716, 819, 896
BspCNI CTCAG 4 cut(s) 595, 969, 1129, 1219
BspEI TCCGGA 1 cut(s) 329
BspHI TCATGA 1 cut(s) 1174
BspLI GGNNCC 6 cut(s) 328, 334, 800, 997, 1104, 1185
BspMAI CTGCAG 1 cut(s) 1258
BspMI ACCTGC 1 cut(s) 786
BspPI GGATC 3 cut(s) 327, 340, 562
BspT104I TTCGAA 2 cut(s) 846, 1283
BspT107I GGYRCC 2 cut(s) 1102, 1183
BspTNI GGTCTC 2 cut(s) 524, 1336
BsrBI CCGCTC 2 cut(s) 174, 343
BsrDI GCAATG 3 cut(s) 907, 1046, 1140
BsrFI RCCGGY 4 cut(s) 425, 491, 896, 1069
BsrI ACTGG 2 cut(s) 356, 642
BssAI RCCGGY 4 cut(s) 425, 491, 896, 1069
BssECI CCNNGG 4 cut(s) 534, 687, 1052, 1164
BssMI GATC 2 cut(s) 332, 567
BssT1I CCWWGG 1 cut(s) 534
Bst2UI CCWGG 3 cut(s) 148, 689, 1165
Bst4CI ACNGT 2 cut(s) 866, 1107
BstBI TTCGAA 2 cut(s) 846, 1283
BstC8I GCNNGC 9 cut(s) 172, 427, 493, 551, 718, 817, 898, 1071, 1191
BstDEI CTNAG 5 cut(s) 582, 977, 1116, 1206, 1308
BstDSI CCRYGG 1 cut(s) 1052
BstF5I GGATG 2 cut(s) 180, 444
BstH2I RGCGCY 1 cut(s) 1076
BstHHI GCGC 3 cut(s) 80, 736, 1075
BstKTI GATC 2 cut(s) 335, 570
BstMAI GTCTC 3 cut(s) 524, 1217, 1336
BstMBI GATC 2 cut(s) 332, 567
BstMCI CGRYCG 1 cut(s) 250
BstNI CCWGG 3 cut(s) 148, 689, 1165
BstSCI CCNGG 5 cut(s) 146, 570, 687, 819, 1163
BstSFI CTRYAG 1 cut(s) 1254
BstV1I GCAGC 6 cut(s) 1062, 1078, 1120, 1240, 1252, 1255
BstV2I GAAGAC 1 cut(s) 623
BstX2I RGATCY 1 cut(s) 332
BstXI CCANNNNNNTGG 2 cut(s) 659, 1053
BstYI RGATCY 1 cut(s) 332
BsuRI GGCC 6 cut(s) 429, 503, 539, 716, 819, 896
BtgI CCRYGG 1 cut(s) 1052
BtsCI GGATG 2 cut(s) 180, 444
BtsI GCAGTG 1 cut(s) 1263
BtsIMutI CAGTG 2 cut(s) 871, 1263
BveI ACCTGC 1 cut(s) 786
Cac8I GCNNGC 9 cut(s) 172, 427, 493, 551, 718, 817, 898, 1071, 1191
CciI TCATGA 1 cut(s) 1174
CfoI GCGC 3 cut(s) 80, 736, 1075
Cfr10I RCCGGY 4 cut(s) 425, 491, 896, 1069
Cfr13I GGNCC 6 cut(s) 4, 538, 574, 714, 777, 818
CseI GACGC 1 cut(s) 1139
CviAII CATG 2 cut(s) 1016, 1175
DdeI CTNAG 5 cut(s) 582, 977, 1116, 1206, 1308
DpnI GATC 2 cut(s) 334, 569
DpnII GATC 2 cut(s) 332, 567
EciI GGCGGA 5 cut(s) 34, 208, 289, 301, 974
Eco130I CCWWGG 1 cut(s) 534
Eco24I GRGCYC 1 cut(s) 687
Eco31I GGTCTC 2 cut(s) 524, 1336
Eco47I GGWCC 3 cut(s) 4, 574, 777
EcoRI GAATTC 1 cut(s) 1111
EcoRII CCWGG 3 cut(s) 146, 687, 1163
EcoT14I CCWWGG 1 cut(s) 534
EcoT38I GRGCYC 1 cut(s) 687
ErhI CCWWGG 1 cut(s) 534
FaeI CATG 2 cut(s) 1019, 1178
FaiI YATR 3 cut(s) 1017, 1161, 1176
FatI CATG 2 cut(s) 1015, 1174
FauI CCCGC 6 cut(s) 104, 179, 185, 262, 348, 449
FokI GGATG 2 cut(s) 167, 431
FriOI GRGCYC 1 cut(s) 687
FspBI CTAG 1 cut(s) 803
GlaI GCGC 3 cut(s) 79, 735, 1074
GsaI CCCAGC 1 cut(s) 885
HaeII RGCGCY 1 cut(s) 1076
HaeIII GGCC 6 cut(s) 429, 503, 539, 716, 819, 896
HgaI GACGC 1 cut(s) 1139
HhaI GCGC 3 cut(s) 80, 736, 1075
Hin1II CATG 2 cut(s) 1019, 1178
Hin6I GCGC 3 cut(s) 78, 734, 1073
HinP1I GCGC 3 cut(s) 78, 734, 1073
HincII GTYRAC 3 cut(s) 705, 974, 1291
HindII GTYRAC 3 cut(s) 705, 974, 1291
HinfI GANTC 9 cut(s) 155, 242, 393, 464, 740, 848, 1025, 1042, 1210
HpaI GTTAAC 1 cut(s) 1291
HphI GGTGA 4 cut(s) 109, 866, 962, 1159
Hpy166II GTNNAC 3 cut(s) 705, 974, 1291
Hpy188I TCNGA 6 cut(s) 247, 337, 379, 463, 728, 1209
Hpy188III TCNNGA 3 cut(s) 330, 1118, 1175
Hpy8I GTNNAC 3 cut(s) 705, 974, 1291
Hpy99I CGWCG 3 cut(s) 131, 134, 401
HpyAV CCTTC 3 cut(s) 219, 376, 418
HpyCH4III ACNGT 2 cut(s) 866, 1107
HpyCH4V TGCA 6 cut(s) 142, 348, 720, 795, 1066, 1256
HpyF3I CTNAG 5 cut(s) 582, 977, 1116, 1206, 1308
Hsp92II CATG 2 cut(s) 1019, 1178
HspAI GCGC 3 cut(s) 78, 734, 1073
Kpn2I TCCGGA 1 cut(s) 329
KroI GCCGGC 4 cut(s) 425, 491, 896, 1069
KroNI GCCGGC 4 cut(s) 427, 493, 898, 1071
KspAI GTTAAC 1 cut(s) 1291
Kzo9I GATC 2 cut(s) 332, 567
LmnI GCTCC 4 cut(s) 179, 332, 513, 1057
Lsp1109I GCAGC 6 cut(s) 1062, 1078, 1120, 1240, 1252, 1255
LweI GCATC 5 cut(s) 189, 453, 756, 1017, 1178
MaeI CTAG 1 cut(s) 803
MalI GATC 2 cut(s) 334, 569
MbiI CCGCTC 2 cut(s) 174, 343
MboI GATC 2 cut(s) 332, 567
MboII GAAGA 4 cut(s) 628, 974, 1051, 1272
MflI RGATCY 1 cut(s) 332
MhlI GDGCHC 2 cut(s) 510, 687
MluCI AATT 5 cut(s) 637, 842, 981, 1111, 1273
MlyI GAGTC 6 cut(s) 164, 236, 387, 458, 1019, 1219
MmeI TCCRAC 4 cut(s) 270, 360, 402, 706
MroI TCCGGA 1 cut(s) 329
MroNI GCCGGC 4 cut(s) 425, 491, 896, 1069
MseI TTAA 2 cut(s) 1125, 1290
MslI CAYNNNNRTG 2 cut(s) 1051, 1145
MspA1I CMGCKG 1 cut(s) 838
MspR9I CCNGG 5 cut(s) 148, 572, 689, 821, 1165
MvaI CCWGG 3 cut(s) 148, 689, 1165
NaeI GCCGGC 4 cut(s) 427, 493, 898, 1071
NciI CCSGG 2 cut(s) 572, 821
NdeII GATC 2 cut(s) 332, 567
NgoMIV GCCGGC 4 cut(s) 425, 491, 896, 1069
NlaIII CATG 2 cut(s) 1019, 1178
NlaIV GGNNCC 6 cut(s) 328, 334, 800, 997, 1104, 1185
NmeAIII GCCGAG 1 cut(s) 553
NspV TTCGAA 2 cut(s) 846, 1283
PagI TCATGA 1 cut(s) 1174
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PdiI GCCGGC 4 cut(s) 427, 493, 898, 1071
PfeI GAWTC 3 cut(s) 740, 848, 1042
PfoI TCCNGGA 1 cut(s) 570
PleI GAGTC 6 cut(s) 163, 236, 387, 458, 1019, 1218
PpsI GAGTC 6 cut(s) 163, 236, 387, 458, 1019, 1218
Psp6I CCWGG 3 cut(s) 146, 687, 1163
PspFI CCCAGC 1 cut(s) 881
PspGI CCWGG 3 cut(s) 146, 687, 1163
PspN4I GGNNCC 6 cut(s) 328, 334, 800, 997, 1104, 1185
PspPI GGNCC 6 cut(s) 4, 538, 574, 714, 777, 818
PstI CTGCAG 1 cut(s) 1258
PsuI RGATCY 1 cut(s) 332
RseI CAYNNNNRTG 2 cut(s) 1051, 1145
SaqAI TTAA 2 cut(s) 1125, 1290
Sau3AI GATC 2 cut(s) 332, 567
Sau96I GGNCC 6 cut(s) 4, 538, 574, 714, 777, 818
SchI GAGTC 6 cut(s) 164, 236, 387, 458, 1019, 1219
ScrFI CCNGG 5 cut(s) 148, 572, 689, 821, 1165
SduI GDGCHC 2 cut(s) 510, 687
SfaNI GCATC 5 cut(s) 189, 453, 756, 1017, 1178
SfcI CTRYAG 1 cut(s) 1254
SfuI TTCGAA 2 cut(s) 846, 1283
SinI GGWCC 3 cut(s) 4, 574, 777
SmiMI CAYNNNNRTG 2 cut(s) 1051, 1145
SmlI CTYRAG 1 cut(s) 680
SmoI CTYRAG 1 cut(s) 680
Sse9I AATT 5 cut(s) 637, 842, 981, 1111, 1273
SspI AATATT 1 cut(s) 1083
SspMI CTAG 1 cut(s) 803
StyD4I CCNGG 5 cut(s) 146, 570, 687, 819, 1163
StyI CCWWGG 1 cut(s) 534
TaaI ACNGT 2 cut(s) 866, 1107
TaqI TCGA 6 cut(s) 204, 279, 566, 607, 846, 1283
TasI AATT 5 cut(s) 637, 842, 981, 1111, 1273
TauI GCSGC 7 cut(s) 293, 527, 676, 679, 841, 1189, 1265
TfiI GAWTC 3 cut(s) 740, 848, 1042
Tru1I TTAA 2 cut(s) 1125, 1290
Tru9I TTAA 2 cut(s) 1125, 1290
TscAI CASTG 2 cut(s) 871, 1263
TseI GCWGC 6 cut(s) 1066, 1075, 1133, 1253, 1265, 1268
TspDTI ATGAA 1 cut(s) 1266
TspGWI ACGGA 1 cut(s) 646
TspRI CASTG 2 cut(s) 871, 1263
VpaK11BI GGWCC 3 cut(s) 4, 574, 777
XapI RAATTY 1 cut(s) 1111
XcmI CCANNNNNNNNNTGG 1 cut(s) 144
XspI CTAG 1 cut(s) 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.