Rh2BG242400

Hyccin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0014272
Physical Location & Seq
Forward (+)
24319827 .. 24321654
1828 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG242400.1

Sequence Viewer

Length: 1371 bp
ATGGACCTCTACCCAAATCACAGCACCAACTCCCCCACCTCCTCCGCCTCCTCCACCGCCACCAACCCAAACCCCAGACCCACCCGCAACACCATATCCTCCCTCACCGCCACCGACGACGACCCAATGCACTCCTGGTGGGAGTCCATCTCCAAAGCCCGCTCCCGCATCCACTCCCTCGCCACTCTCCTCGACCCTTCTCTCTCCTCCTCCCTCTCCTCCCTCGCCGACTCCGACCGCCCCGCCCTCTCTCTCCTCTCCTCCCTCGAAGCCTACGCCGCCGTTTCCGCCGTCCTCTCCGCCCCCCTCTCCGGCTCCGGATCCGACCCGCTCTGCAACTGGCTCTACGACACCTTTCTCTCCTCCGACCCCCATCTACGACTCGTCGTTTTCTCCTTCCTCCCTCTCCTCGCCGGCCTCTACCTCTCCCGCATCCACTCTCTCTCCTCTGACTCTCTTACCTCGCTTCCCTCCCTCGCCGGCTTTGAGGCCGTGCTCCTCGCTCTCTACGCCGCCGAGACCAAGGCCCGAAACGGCAAGCCCATTGTCGTTTCGATCCCAGACCTATCTCAGCCCTCTCTCTACCACATTCCTCGAAGCCAGAAGACGACTAATCCCAACTCCAATTCCAGTAATATCCAATCCGTTGGGGTTTTGTCGCCGCCGCTGGAGCCCCAGGTCGCGGTCAAGTCAACCAAACGGGCCTGCATTGTGGGAGTTGCGCTGGATTCTTATTACAAGCACATTTCGCAGATGCCCACTTGGTCCAAGATGGATTTTTGCAGGTTCCTAGCTTCTTGGGCCGGCCCGGATTGCTCTTGTCAGCGGCAATTTGAATCCGGTGATAACCACAGTGAGCCAGAGATTGCTGGGTTCTTGGAGGCCGGCAATGGGAATGGGAGTGGTAATGAGAATGAGAATGAGAATGAGAATGAGAATGAGAATGAGGGTGAGAGTGAGAGTGAGATCGAGATTGAGGGTGATGTGGCGGAAGAAATGGGTCAACTAAGAATTGAGCAAAATGGGATCAGCAGTGGGGTCAGTCATGTGGATGACTCAAAGGGGGCGAAGATTCCATTGCCGTGGGAGCTTTTGCAGCCAGCGCTGCGAATATTGGGGCATTGTTTGTTGGCGCCGTTGAATTCTCAGGAGGTTAAGGACGCTGCTTCCATTGCGGTGAGGAGATTGTATGCCAGGGCATCTCATGATTTGGTGCCGCAGGCGATTTTAGCTACTCAGAGTCTCATTCAGCTTGATAACAGGGGGCGTGAGGCAGCCAAGGATGCAGTGGCGGTTGCTGCTAATTCATCTTCGAATGTTAACACGCCGAGCAAAGCTAAGAAACCTGAAATCCTTTTGGTCTCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hyccin

Protein Analysis

456

Amino Acids

48.88

Weight (kDa)

5.15

Isoelectric Point (pI)

52.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hyccin PF09790 77 - 418 1.7e-61 Hyccin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64090
fragaria_vesca FvH4_1g19990
malus_domestica MD05G1042400.v1.1 MD10G1049000.v1.1
prunus_persica Prupe.8G059200_v2.0.a1
pyrus_communis pycom05g03500
rosa_chinensis RchiOBHm_Chr2g0111931
rosa_laevigata RLG00000017939 RLG00000034552
rosa_multiflora Rmu_sc0005679.1_g000010
rosa_roxburghii Rroxscaffold_2G00131980
rosa_rugosa Rorug02G0177300
rosa_samantha Rh2AG229700 Rh2BG242400 Rh2CG233800 Rh2DG237400
rosa_wichuraiana Rw2G017770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 774
AccB1I GGYRCC 2 cut(s) 1132, 1213
AccBSI CCGCTC 2 cut(s) 162, 331
AccII CGCG 1 cut(s) 683
AccIII TCCGGA 1 cut(s) 317
AclWI GGATC 4 cut(s) 315, 328, 550, 1034
AcsI RAATTY 1 cut(s) 1141
AcyI GRCGYC 1 cut(s) 1133
AfeI AGCGCT 1 cut(s) 1104
AfiI CCNNNNNNNGG 3 cut(s) 311, 682, 891
AgsI TTSAA 2 cut(s) 836, 1141
AjnI CCWGG 3 cut(s) 134, 675, 1193
AluBI AGCT 5 cut(s) 794, 1090, 1232, 1252, 1337
AluI AGCT 5 cut(s) 794, 1090, 1232, 1252, 1337
Alw21I GWGCWC 1 cut(s) 498
Alw26I GTCTC 3 cut(s) 512, 1247, 1366
AlwI GGATC 4 cut(s) 315, 328, 550, 1034
Aor13HI TCCGGA 1 cut(s) 317
Aor51HI AGCGCT 1 cut(s) 1104
AoxI GGCC 7 cut(s) 415, 489, 525, 702, 801, 805, 882
ApeKI GCWGC 5 cut(s) 1096, 1105, 1163, 1274, 1298
ApoI RAATTY 1 cut(s) 1141
AspLEI GCGC 3 cut(s) 724, 1105, 1135
AspS9I GGNCC 6 cut(s) 4, 526, 702, 765, 801, 806
AsuC2I CCSGG 1 cut(s) 809
AsuHPI GGTGA 5 cut(s) 97, 854, 962, 992, 1189
AsuII TTCGAA 1 cut(s) 1313
AvaII GGWCC 2 cut(s) 4, 765
BamHI GGATCC 1 cut(s) 320
BanI GGYRCC 2 cut(s) 1132, 1213
BanII GRGCYC 1 cut(s) 675
BbsI GAAGAC 1 cut(s) 611
Bbv12I GWGCWC 1 cut(s) 498
BbvI GCAGC 5 cut(s) 1092, 1108, 1150, 1285, 1286
BccI CCATC 3 cut(s) 155, 381, 766
BceAI ACGGC 6 cut(s) 266, 275, 476, 550, 1066, 1120
BciT130I CCWGG 3 cut(s) 136, 677, 1195
BcnI CCSGG 1 cut(s) 809
BcoDI GTCTC 3 cut(s) 512, 1247, 1366
BfaI CTAG 1 cut(s) 791
BfoI RGCGCY 2 cut(s) 1106, 1136
BfuAI ACCTGC 1 cut(s) 774
Bme1390I CCNGG 4 cut(s) 136, 677, 809, 1195
Bme18I GGWCC 2 cut(s) 4, 765
BmgT120I GGNCC 6 cut(s) 4, 526, 702, 765, 801, 806
BmiI GGNNCC 6 cut(s) 316, 322, 672, 788, 1134, 1215
BmrFI CCNGG 4 cut(s) 136, 677, 809, 1195
BmsI GCATC 5 cut(s) 177, 441, 744, 1208, 1273
BpiI GAAGAC 1 cut(s) 611
BplI GAGNNNNNCTC 2 cut(s) 134, 166
BpmI CTGGAG 1 cut(s) 689
Bpu14I TTCGAA 1 cut(s) 1313
BpuMI CCSGG 1 cut(s) 809
BsaHI GRCGYC 1 cut(s) 1133
BsaI GGTCTC 2 cut(s) 512, 1366
BsaJI CCNNGG 5 cut(s) 522, 675, 1082, 1194, 1278
BsaWI WCCGGW 2 cut(s) 317, 839
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bsc4I CCNNNNNNNGG 3 cut(s) 311, 682, 891
Bse118I RCCGGY 4 cut(s) 413, 479, 803, 884
Bse1I ACTGG 2 cut(s) 344, 630
Bse3DI GCAATG 3 cut(s) 895, 1076, 1170
BseAI TCCGGA 1 cut(s) 317
BseBI CCWGG 3 cut(s) 136, 677, 1195
BseDI CCNNGG 5 cut(s) 522, 675, 1082, 1194, 1278
BseGI GGATG 4 cut(s) 168, 432, 1057, 1288
BseLI CCNNNNNNNGG 3 cut(s) 311, 682, 891
BseMI GCAATG 3 cut(s) 895, 1076, 1170
BseMII CTCAG 3 cut(s) 584, 1160, 1250
BseNI ACTGG 2 cut(s) 344, 630
BseXI GCAGC 5 cut(s) 1092, 1108, 1150, 1285, 1286
BseYI CCCAGC 1 cut(s) 869
Bsh1236I CGCG 1 cut(s) 683
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 7 cut(s) 417, 491, 527, 704, 803, 807, 884
BshNI GGYRCC 2 cut(s) 1132, 1213
BsiEI CGRYCG 1 cut(s) 238
BsiHKAI GWGCWC 1 cut(s) 498
BsiSI CCGG 8 cut(s) 312, 318, 414, 480, 804, 809, 840, 885
BslI CCNNNNNNNGG 3 cut(s) 311, 682, 891
BsmAI GTCTC 3 cut(s) 512, 1247, 1366
BsnI GGCC 7 cut(s) 417, 491, 527, 704, 803, 807, 884
Bso31I GGTCTC 2 cut(s) 512, 1366
Bsp119I TTCGAA 1 cut(s) 1313
Bsp1286I GDGCHC 2 cut(s) 498, 675
Bsp13I TCCGGA 1 cut(s) 317
Bsp143I GATC 4 cut(s) 320, 555, 966, 1026
BspANI GGCC 7 cut(s) 417, 491, 527, 704, 803, 807, 884
BspCNI CTCAG 3 cut(s) 583, 1159, 1249
BspEI TCCGGA 1 cut(s) 317
BspFNI CGCG 1 cut(s) 683
BspHI TCATGA 1 cut(s) 1204
BspLI GGNNCC 6 cut(s) 316, 322, 672, 788, 1134, 1215
BspMI ACCTGC 1 cut(s) 774
BspPI GGATC 4 cut(s) 315, 328, 550, 1034
BspT104I TTCGAA 1 cut(s) 1313
BspT107I GGYRCC 2 cut(s) 1132, 1213
BspTNI GGTCTC 2 cut(s) 512, 1366
BsrBI CCGCTC 2 cut(s) 162, 331
BsrDI GCAATG 3 cut(s) 895, 1076, 1170
BsrFI RCCGGY 4 cut(s) 413, 479, 803, 884
BsrI ACTGG 2 cut(s) 344, 630
BssAI RCCGGY 4 cut(s) 413, 479, 803, 884
BssECI CCNNGG 5 cut(s) 522, 675, 1082, 1194, 1278
BssMI GATC 4 cut(s) 320, 555, 966, 1026
BssNI GRCGYC 1 cut(s) 1133
BssT1I CCWWGG 2 cut(s) 522, 1278
Bst2UI CCWGG 3 cut(s) 136, 677, 1195
Bst4CI ACNGT 1 cut(s) 854
BstACI GRCGYC 1 cut(s) 1133
BstBI TTCGAA 1 cut(s) 1313
BstC8I GCNNGC 9 cut(s) 160, 415, 481, 539, 706, 805, 886, 1101, 1221
BstDEI CTNAG 5 cut(s) 570, 1007, 1146, 1236, 1338
BstDSI CCRYGG 1 cut(s) 1082
BstF5I GGATG 4 cut(s) 168, 432, 1057, 1288
BstFNI CGCG 1 cut(s) 683
BstH2I RGCGCY 2 cut(s) 1106, 1136
BstHHI GCGC 3 cut(s) 724, 1105, 1135
BstKTI GATC 4 cut(s) 323, 558, 969, 1029
BstMAI GTCTC 3 cut(s) 512, 1247, 1366
BstMBI GATC 4 cut(s) 320, 555, 966, 1026
BstMCI CGRYCG 1 cut(s) 238
BstNI CCWGG 3 cut(s) 136, 677, 1195
BstSCI CCNGG 4 cut(s) 134, 675, 807, 1193
BstUI CGCG 1 cut(s) 683
BstV1I GCAGC 5 cut(s) 1092, 1108, 1150, 1285, 1286
BstV2I GAAGAC 1 cut(s) 611
BstX2I RGATCY 1 cut(s) 320
BstXI CCANNNNNNTGG 2 cut(s) 647, 1083
BstYI RGATCY 1 cut(s) 320
BsuRI GGCC 7 cut(s) 417, 491, 527, 704, 803, 807, 884
BtgI CCRYGG 1 cut(s) 1082
BtsCI GGATG 4 cut(s) 168, 432, 1057, 1288
BtsI GCAGTG 2 cut(s) 1039, 1293
BtsIMutI CAGTG 3 cut(s) 859, 1039, 1293
BveI ACCTGC 1 cut(s) 774
Cac8I GCNNGC 9 cut(s) 160, 415, 481, 539, 706, 805, 886, 1101, 1221
CciI TCATGA 1 cut(s) 1204
CfoI GCGC 3 cut(s) 724, 1105, 1135
Cfr10I RCCGGY 4 cut(s) 413, 479, 803, 884
Cfr13I GGNCC 6 cut(s) 4, 526, 702, 765, 801, 806
CseI GACGC 1 cut(s) 1169
CviAII CATG 2 cut(s) 1046, 1205
DdeI CTNAG 5 cut(s) 570, 1007, 1146, 1236, 1338
DinI GGCGCC 1 cut(s) 1134
DpnI GATC 4 cut(s) 322, 557, 968, 1028
DpnII GATC 4 cut(s) 320, 555, 966, 1026
EciI GGCGGA 4 cut(s) 34, 277, 289, 1004
Eco130I CCWWGG 2 cut(s) 522, 1278
Eco24I GRGCYC 1 cut(s) 675
Eco31I GGTCTC 2 cut(s) 512, 1366
Eco47I GGWCC 2 cut(s) 4, 765
Eco47III AGCGCT 1 cut(s) 1104
EcoRI GAATTC 1 cut(s) 1141
EcoRII CCWGG 3 cut(s) 134, 675, 1193
EcoT14I CCWWGG 2 cut(s) 522, 1278
EcoT38I GRGCYC 1 cut(s) 675
EgeI GGCGCC 1 cut(s) 1134
EheI GGCGCC 1 cut(s) 1134
ErhI CCWWGG 2 cut(s) 522, 1278
FaeI CATG 2 cut(s) 1049, 1208
FaiI YATR 4 cut(s) 95, 1047, 1191, 1206
FatI CATG 2 cut(s) 1045, 1204
FauI CCCGC 6 cut(s) 92, 167, 173, 250, 336, 437
FokI GGATG 4 cut(s) 155, 419, 1064, 1295
FriOI GRGCYC 1 cut(s) 675
FseI GGCCGGCC 1 cut(s) 807
FspBI CTAG 1 cut(s) 791
GlaI GCGC 3 cut(s) 723, 1104, 1134
GsaI CCCAGC 1 cut(s) 873
GsuI CTGGAG 1 cut(s) 689
HaeII RGCGCY 2 cut(s) 1106, 1136
HaeIII GGCC 7 cut(s) 417, 491, 527, 704, 803, 807, 884
HapII CCGG 8 cut(s) 312, 318, 414, 480, 804, 809, 840, 885
HgaI GACGC 1 cut(s) 1169
HhaI GCGC 3 cut(s) 724, 1105, 1135
Hin1I GRCGYC 1 cut(s) 1133
Hin1II CATG 2 cut(s) 1049, 1208
Hin6I GCGC 3 cut(s) 722, 1103, 1133
HinP1I GCGC 3 cut(s) 722, 1103, 1133
HincII GTYRAC 3 cut(s) 693, 1004, 1321
HindII GTYRAC 3 cut(s) 693, 1004, 1321
HinfI GANTC 9 cut(s) 143, 230, 381, 452, 728, 836, 1055, 1072, 1240
HpaI GTTAAC 1 cut(s) 1321
HpaII CCGG 8 cut(s) 312, 318, 414, 480, 804, 809, 840, 885
HphI GGTGA 5 cut(s) 97, 854, 962, 992, 1189
Hpy166II GTNNAC 3 cut(s) 693, 1004, 1321
Hpy188I TCNGA 5 cut(s) 235, 325, 367, 451, 1239
Hpy188III TCNNGA 4 cut(s) 318, 970, 1148, 1205
Hpy8I GTNNAC 3 cut(s) 693, 1004, 1321
Hpy99I CGWCG 3 cut(s) 119, 122, 389
HpyAV CCTTC 2 cut(s) 207, 406
HpyCH4III ACNGT 1 cut(s) 854
HpyCH4V TGCA 6 cut(s) 130, 336, 708, 783, 1096, 1286
HpyF3I CTNAG 5 cut(s) 570, 1007, 1146, 1236, 1338
Hsp92I GRCGYC 1 cut(s) 1133
Hsp92II CATG 2 cut(s) 1049, 1208
HspAI GCGC 3 cut(s) 722, 1103, 1133
KasI GGCGCC 1 cut(s) 1132
Kpn2I TCCGGA 1 cut(s) 317
KroI GCCGGC 4 cut(s) 413, 479, 803, 884
KroNI GCCGGC 4 cut(s) 415, 481, 805, 886
KspAI GTTAAC 1 cut(s) 1321
Kzo9I GATC 4 cut(s) 320, 555, 966, 1026
LmnI GCTCC 5 cut(s) 167, 320, 501, 670, 1087
Lsp1109I GCAGC 5 cut(s) 1092, 1108, 1150, 1285, 1286
LweI GCATC 5 cut(s) 177, 441, 744, 1208, 1273
MaeI CTAG 1 cut(s) 791
MalI GATC 4 cut(s) 322, 557, 968, 1028
MbiI CCGCTC 2 cut(s) 162, 331
MboI GATC 4 cut(s) 320, 555, 966, 1026
MboII GAAGA 4 cut(s) 616, 1004, 1081, 1302
MflI RGATCY 1 cut(s) 320
MhlI GDGCHC 2 cut(s) 498, 675
MluCI AATT 5 cut(s) 625, 830, 1011, 1141, 1303
Mly113I GGCGCC 1 cut(s) 1133
MlyI GAGTC 6 cut(s) 152, 224, 375, 446, 1049, 1249
MmeI TCCRAC 3 cut(s) 258, 348, 390
MroI TCCGGA 1 cut(s) 317
MroNI GCCGGC 4 cut(s) 413, 479, 803, 884
MseI TTAA 2 cut(s) 1155, 1320
MslI CAYNNNNRTG 3 cut(s) 1050, 1081, 1175
MspA1I CMGCKG 2 cut(s) 667, 826
MspI CCGG 8 cut(s) 312, 318, 414, 480, 804, 809, 840, 885
MspR9I CCNGG 4 cut(s) 136, 677, 809, 1195
MvaI CCWGG 3 cut(s) 136, 677, 1195
MvnI CGCG 1 cut(s) 683
NaeI GCCGGC 4 cut(s) 415, 481, 805, 886
NarI GGCGCC 1 cut(s) 1133
NciI CCSGG 1 cut(s) 809
NdeII GATC 4 cut(s) 320, 555, 966, 1026
NgoMIV GCCGGC 4 cut(s) 413, 479, 803, 884
NlaIII CATG 2 cut(s) 1049, 1208
NlaIV GGNNCC 6 cut(s) 316, 322, 672, 788, 1134, 1215
NmeAIII GCCGAG 2 cut(s) 541, 1353
NspV TTCGAA 1 cut(s) 1313
PagI TCATGA 1 cut(s) 1204
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PdiI GCCGGC 4 cut(s) 415, 481, 805, 886
PfeI GAWTC 3 cut(s) 728, 836, 1072
PleI GAGTC 6 cut(s) 151, 224, 375, 446, 1049, 1248
PluTI GGCGCC 1 cut(s) 1136
PpsI GAGTC 6 cut(s) 151, 224, 375, 446, 1049, 1248
Psp6I CCWGG 3 cut(s) 134, 675, 1193
PspFI CCCAGC 1 cut(s) 869
PspGI CCWGG 3 cut(s) 134, 675, 1193
PspN4I GGNNCC 6 cut(s) 316, 322, 672, 788, 1134, 1215
PspPI GGNCC 6 cut(s) 4, 526, 702, 765, 801, 806
PsuI RGATCY 1 cut(s) 320
RigI GGCCGGCC 1 cut(s) 807
RseI CAYNNNNRTG 3 cut(s) 1050, 1081, 1175
SaqAI TTAA 2 cut(s) 1155, 1320
Sau3AI GATC 4 cut(s) 320, 555, 966, 1026
Sau96I GGNCC 6 cut(s) 4, 526, 702, 765, 801, 806
SchI GAGTC 6 cut(s) 152, 224, 375, 446, 1049, 1249
ScrFI CCNGG 4 cut(s) 136, 677, 809, 1195
SduI GDGCHC 2 cut(s) 498, 675
SfaNI GCATC 5 cut(s) 177, 441, 744, 1208, 1273
SfoI GGCGCC 1 cut(s) 1134
SfuI TTCGAA 1 cut(s) 1313
SinI GGWCC 2 cut(s) 4, 765
SmiMI CAYNNNNRTG 3 cut(s) 1050, 1081, 1175
Sse9I AATT 5 cut(s) 625, 830, 1011, 1141, 1303
SspDI GGCGCC 1 cut(s) 1132
SspI AATATT 1 cut(s) 1113
SspMI CTAG 1 cut(s) 791
StyD4I CCNGG 4 cut(s) 134, 675, 807, 1193
StyI CCWWGG 2 cut(s) 522, 1278
TaaI ACNGT 1 cut(s) 854
TaqI TCGA 6 cut(s) 192, 267, 554, 595, 969, 1313
TasI AATT 5 cut(s) 625, 830, 1011, 1141, 1303
TauI GCSGC 6 cut(s) 281, 515, 664, 667, 829, 1219
TfiI GAWTC 3 cut(s) 728, 836, 1072
Tru1I TTAA 2 cut(s) 1155, 1320
Tru9I TTAA 2 cut(s) 1155, 1320
TscAI CASTG 3 cut(s) 859, 1039, 1293
TseI GCWGC 5 cut(s) 1096, 1105, 1163, 1274, 1298
TspDTI ATGAA 1 cut(s) 1296
TspGWI ACGGA 1 cut(s) 634
TspRI CASTG 3 cut(s) 859, 1039, 1293
VpaK11BI GGWCC 2 cut(s) 4, 765
XapI RAATTY 1 cut(s) 1141
XcmI CCANNNNNNNNNTGG 2 cut(s) 132, 1285
XspI CTAG 1 cut(s) 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.