Rh2AG229700

Hyccin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0014272
Physical Location & Seq
Forward (+)
24454883 .. 24457708
2826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG229700.1

Sequence Viewer

Length: 1341 bp
ATGGACCTCTACCCAAATCACAGCACCAACTCCCCCACCTCCTCCGCCTCCTCCACCGCCACCAACCCAAACCCAAACCCCAGACCCACCCGCAACACCATCTCCTCCCTCACCGCCACCGACGACGACCCAATGCACTCCTGGTGGGAGTCCATCTCCAAAGCCCGCTCCCGCATCCACTCCCTCGCCACTCTCCTCGACCCTTCTCTCTCCTCCTCCCTCTCCTCCCTCGCCGACTCCGACCGCCCCGCCCTCTCTCTCCTCTCCTCCCTCGAAGCCTACGCCGCCATTTCCGCCGTCCTCTCCGCCCCCCTCTCCGGCTCCGGATCCGACCCGCTCTGCAACTGGCTCTACGACACCTTCCTCTCCTCCGACTCCCATCTACGACTCGTCGTTTTCTCCTTCCTCCCTCTCCTCGCCGGCCTCTACCTCTCCCGCATCCACTCTCTCTCCTCTGACTCTCTTACCTCGCTTCCCTCCCTCGCCGGCTTTGAGGCCGTGCTCCTCGCTCTCTACGCCGCCGAGACCAAGGCCCGAAACGGCAAGCCCATTGTCGTTTCGATCCCAGACCTATCTCAGCCCTCTCTCTACCACATTCCTCGAAGCCAGAAGACGACTAATCCCAACTCCAATTCCAGTAATATCCAATCCGTTGGGGTTTTGTCGCCGCCGCTGGAGCCCCAGGTCGCGGTCAAGTCAACCAAACGGGCCTGCATTGTGGGAGTTGCGCTGGATTCTTATTACAAGCACATTTCGCAGATGCCCACTTGGTCCAAGATGGATTTTTGCAGGTTCCTAGCTTCTTGGGCCGGCCCGGATTGCTCTTGTCAGCGGCAATTTGAATCCGGTGATAACCACAGTGAGCCAGAGATTGCTGGGTTCTTGGAGGCCGGCAATGGGAATGGGAGTGGTAATGAGAATGAGAGTGAGAGTGAGATTGAGATTGAGGGTGATGTGGCGGAAGAAATGGGTCAACTAAGAATTGAGCACAATGGGATCAGCAGTGGGGTCAGTCATGTGGAGGACTCAAAGGGGGCGAAGATTCCATTGCCGTGGGAGCTTTTGCAGCCAGCGCTGCGAATATTGGGGCATTGTTTGTTGGCGCCGTTGAATTCTCAGGAGGTTAAGGACGCTGCTTCCATTGCGGTGAGGAGATTGTATGCCAGGGCATCTCATGATTTGGTGCCGCAGGCGATTTTAGCTACTCAGAGTCTCATTCAGCTTGATAACAGGGGGCGTGAGGCAGCCAAGGATGCAGTGGCGGTTGCTGCTAATTCATCTTCGAATGTTAACACGCCGAGCAAAGCTAAGAAACCTGAAATCCTTTTGGTCTCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hyccin

Protein Analysis

446

Amino Acids

47.72

Weight (kDa)

5.65

Isoelectric Point (pI)

54.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hyccin PF09790 79 - 408 9.2e-61 Hyccin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64090
fragaria_vesca FvH4_1g19990
malus_domestica MD05G1042400.v1.1 MD10G1049000.v1.1
prunus_persica Prupe.8G059200_v2.0.a1
pyrus_communis pycom05g03500
rosa_chinensis RchiOBHm_Chr2g0111931
rosa_laevigata RLG00000017939 RLG00000034552
rosa_multiflora Rmu_sc0005679.1_g000010
rosa_roxburghii Rroxscaffold_2G00131980
rosa_rugosa Rorug02G0177300
rosa_samantha Rh2AG229700 Rh2BG242400 Rh2CG233800 Rh2DG237400
rosa_wichuraiana Rw2G017770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 780
AccB1I GGYRCC 2 cut(s) 1102, 1183
AccBSI CCGCTC 2 cut(s) 168, 337
AccII CGCG 1 cut(s) 689
AccIII TCCGGA 1 cut(s) 323
AclWI GGATC 4 cut(s) 321, 334, 556, 1004
AcsI RAATTY 1 cut(s) 1111
AcyI GRCGYC 1 cut(s) 1103
AfeI AGCGCT 1 cut(s) 1074
AfiI CCNNNNNNNGG 3 cut(s) 317, 688, 897
AgsI TTSAA 2 cut(s) 842, 1111
AjnI CCWGG 3 cut(s) 140, 681, 1163
AluBI AGCT 5 cut(s) 800, 1060, 1202, 1222, 1307
AluI AGCT 5 cut(s) 800, 1060, 1202, 1222, 1307
Alw21I GWGCWC 2 cut(s) 504, 990
Alw26I GTCTC 3 cut(s) 518, 1217, 1336
AlwI GGATC 4 cut(s) 321, 334, 556, 1004
Aor13HI TCCGGA 1 cut(s) 323
Aor51HI AGCGCT 1 cut(s) 1074
AoxI GGCC 7 cut(s) 421, 495, 531, 708, 807, 811, 888
ApeKI GCWGC 5 cut(s) 1066, 1075, 1133, 1244, 1268
ApoI RAATTY 1 cut(s) 1111
AspLEI GCGC 3 cut(s) 730, 1075, 1105
AspS9I GGNCC 6 cut(s) 4, 532, 708, 771, 807, 812
AsuC2I CCSGG 1 cut(s) 815
AsuHPI GGTGA 4 cut(s) 103, 860, 962, 1159
AsuII TTCGAA 1 cut(s) 1283
AvaII GGWCC 2 cut(s) 4, 771
BamHI GGATCC 1 cut(s) 326
BanI GGYRCC 2 cut(s) 1102, 1183
BanII GRGCYC 1 cut(s) 681
BbsI GAAGAC 1 cut(s) 617
Bbv12I GWGCWC 2 cut(s) 504, 990
BbvI GCAGC 5 cut(s) 1062, 1078, 1120, 1255, 1256
BccI CCATC 4 cut(s) 107, 161, 387, 772
BceAI ACGGC 5 cut(s) 281, 482, 556, 1036, 1090
BciT130I CCWGG 3 cut(s) 142, 683, 1165
BcnI CCSGG 1 cut(s) 815
BcoDI GTCTC 3 cut(s) 518, 1217, 1336
BfaI CTAG 1 cut(s) 797
BfoI RGCGCY 2 cut(s) 1076, 1106
BfuAI ACCTGC 1 cut(s) 780
Bme1390I CCNGG 4 cut(s) 142, 683, 815, 1165
Bme18I GGWCC 2 cut(s) 4, 771
BmgT120I GGNCC 6 cut(s) 4, 532, 708, 771, 807, 812
BmiI GGNNCC 6 cut(s) 322, 328, 678, 794, 1104, 1185
BmrFI CCNGG 4 cut(s) 142, 683, 815, 1165
BmsI GCATC 5 cut(s) 183, 447, 750, 1178, 1243
BpiI GAAGAC 1 cut(s) 617
BplI GAGNNNNNCTC 2 cut(s) 140, 172
BpmI CTGGAG 1 cut(s) 695
Bpu14I TTCGAA 1 cut(s) 1283
BpuMI CCSGG 1 cut(s) 815
BsaHI GRCGYC 1 cut(s) 1103
BsaI GGTCTC 2 cut(s) 518, 1336
BsaJI CCNNGG 5 cut(s) 528, 681, 1052, 1164, 1248
BsaWI WCCGGW 2 cut(s) 323, 845
BsaXI ACNNNNNCTCC 4 cut(s) 86, 116, 434, 464
Bsc4I CCNNNNNNNGG 3 cut(s) 317, 688, 897
Bse118I RCCGGY 4 cut(s) 419, 485, 809, 890
Bse1I ACTGG 2 cut(s) 350, 636
Bse3DI GCAATG 3 cut(s) 901, 1046, 1140
BseAI TCCGGA 1 cut(s) 323
BseBI CCWGG 3 cut(s) 142, 683, 1165
BseDI CCNNGG 5 cut(s) 528, 681, 1052, 1164, 1248
BseGI GGATG 3 cut(s) 174, 438, 1258
BseLI CCNNNNNNNGG 3 cut(s) 317, 688, 897
BseMI GCAATG 3 cut(s) 901, 1046, 1140
BseMII CTCAG 3 cut(s) 590, 1130, 1220
BseNI ACTGG 2 cut(s) 350, 636
BseXI GCAGC 5 cut(s) 1062, 1078, 1120, 1255, 1256
BseYI CCCAGC 1 cut(s) 875
Bsh1236I CGCG 1 cut(s) 689
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 7 cut(s) 423, 497, 533, 710, 809, 813, 890
BshNI GGYRCC 2 cut(s) 1102, 1183
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 2 cut(s) 504, 990
BsiSI CCGG 8 cut(s) 318, 324, 420, 486, 810, 815, 846, 891
BslI CCNNNNNNNGG 3 cut(s) 317, 688, 897
BsmAI GTCTC 3 cut(s) 518, 1217, 1336
BsnI GGCC 7 cut(s) 423, 497, 533, 710, 809, 813, 890
Bso31I GGTCTC 2 cut(s) 518, 1336
Bsp119I TTCGAA 1 cut(s) 1283
Bsp1286I GDGCHC 3 cut(s) 504, 681, 990
Bsp13I TCCGGA 1 cut(s) 323
Bsp143I GATC 3 cut(s) 326, 561, 996
BspANI GGCC 7 cut(s) 423, 497, 533, 710, 809, 813, 890
BspCNI CTCAG 3 cut(s) 589, 1129, 1219
BspEI TCCGGA 1 cut(s) 323
BspFNI CGCG 1 cut(s) 689
BspHI TCATGA 1 cut(s) 1174
BspLI GGNNCC 6 cut(s) 322, 328, 678, 794, 1104, 1185
BspMI ACCTGC 1 cut(s) 780
BspPI GGATC 4 cut(s) 321, 334, 556, 1004
BspT104I TTCGAA 1 cut(s) 1283
BspT107I GGYRCC 2 cut(s) 1102, 1183
BspTNI GGTCTC 2 cut(s) 518, 1336
BsrBI CCGCTC 2 cut(s) 168, 337
BsrDI GCAATG 3 cut(s) 901, 1046, 1140
BsrFI RCCGGY 4 cut(s) 419, 485, 809, 890
BsrI ACTGG 2 cut(s) 350, 636
BssAI RCCGGY 4 cut(s) 419, 485, 809, 890
BssECI CCNNGG 5 cut(s) 528, 681, 1052, 1164, 1248
BssMI GATC 3 cut(s) 326, 561, 996
BssNI GRCGYC 1 cut(s) 1103
BssT1I CCWWGG 2 cut(s) 528, 1248
Bst2UI CCWGG 3 cut(s) 142, 683, 1165
Bst4CI ACNGT 1 cut(s) 860
BstACI GRCGYC 1 cut(s) 1103
BstBI TTCGAA 1 cut(s) 1283
BstC8I GCNNGC 9 cut(s) 166, 421, 487, 545, 712, 811, 892, 1071, 1191
BstDEI CTNAG 5 cut(s) 576, 977, 1116, 1206, 1308
BstDSI CCRYGG 1 cut(s) 1052
BstF5I GGATG 3 cut(s) 174, 438, 1258
BstFNI CGCG 1 cut(s) 689
BstH2I RGCGCY 2 cut(s) 1076, 1106
BstHHI GCGC 3 cut(s) 730, 1075, 1105
BstKTI GATC 3 cut(s) 329, 564, 999
BstMAI GTCTC 3 cut(s) 518, 1217, 1336
BstMBI GATC 3 cut(s) 326, 561, 996
BstMCI CGRYCG 1 cut(s) 244
BstNI CCWGG 3 cut(s) 142, 683, 1165
BstSCI CCNGG 4 cut(s) 140, 681, 813, 1163
BstUI CGCG 1 cut(s) 689
BstV1I GCAGC 5 cut(s) 1062, 1078, 1120, 1255, 1256
BstV2I GAAGAC 1 cut(s) 617
BstX2I RGATCY 1 cut(s) 326
BstXI CCANNNNNNTGG 2 cut(s) 653, 1053
BstYI RGATCY 1 cut(s) 326
BsuRI GGCC 7 cut(s) 423, 497, 533, 710, 809, 813, 890
BtgI CCRYGG 1 cut(s) 1052
BtsCI GGATG 3 cut(s) 174, 438, 1258
BtsI GCAGTG 2 cut(s) 1009, 1263
BtsIMutI CAGTG 3 cut(s) 865, 1009, 1263
BveI ACCTGC 1 cut(s) 780
Cac8I GCNNGC 9 cut(s) 166, 421, 487, 545, 712, 811, 892, 1071, 1191
CciI TCATGA 1 cut(s) 1174
CfoI GCGC 3 cut(s) 730, 1075, 1105
Cfr10I RCCGGY 4 cut(s) 419, 485, 809, 890
Cfr13I GGNCC 6 cut(s) 4, 532, 708, 771, 807, 812
CseI GACGC 1 cut(s) 1139
CviAII CATG 2 cut(s) 1016, 1175
DdeI CTNAG 5 cut(s) 576, 977, 1116, 1206, 1308
DinI GGCGCC 1 cut(s) 1104
DpnI GATC 3 cut(s) 328, 563, 998
DpnII GATC 3 cut(s) 326, 561, 996
EciI GGCGGA 4 cut(s) 34, 283, 295, 974
Eco130I CCWWGG 2 cut(s) 528, 1248
Eco24I GRGCYC 1 cut(s) 681
Eco31I GGTCTC 2 cut(s) 518, 1336
Eco47I GGWCC 2 cut(s) 4, 771
Eco47III AGCGCT 1 cut(s) 1074
EcoRI GAATTC 1 cut(s) 1111
EcoRII CCWGG 3 cut(s) 140, 681, 1163
EcoT14I CCWWGG 2 cut(s) 528, 1248
EcoT38I GRGCYC 1 cut(s) 681
EgeI GGCGCC 1 cut(s) 1104
EheI GGCGCC 1 cut(s) 1104
ErhI CCWWGG 2 cut(s) 528, 1248
FaeI CATG 2 cut(s) 1019, 1178
FaiI YATR 3 cut(s) 1017, 1161, 1176
FatI CATG 2 cut(s) 1015, 1174
FauI CCCGC 6 cut(s) 98, 173, 179, 256, 342, 443
FokI GGATG 3 cut(s) 161, 425, 1265
FriOI GRGCYC 1 cut(s) 681
FseI GGCCGGCC 1 cut(s) 813
FspBI CTAG 1 cut(s) 797
GlaI GCGC 3 cut(s) 729, 1074, 1104
GsaI CCCAGC 1 cut(s) 879
GsuI CTGGAG 1 cut(s) 695
HaeII RGCGCY 2 cut(s) 1076, 1106
HaeIII GGCC 7 cut(s) 423, 497, 533, 710, 809, 813, 890
HapII CCGG 8 cut(s) 318, 324, 420, 486, 810, 815, 846, 891
HgaI GACGC 1 cut(s) 1139
HhaI GCGC 3 cut(s) 730, 1075, 1105
Hin1I GRCGYC 1 cut(s) 1103
Hin1II CATG 2 cut(s) 1019, 1178
Hin6I GCGC 3 cut(s) 728, 1073, 1103
HinP1I GCGC 3 cut(s) 728, 1073, 1103
HincII GTYRAC 3 cut(s) 699, 974, 1291
HindII GTYRAC 3 cut(s) 699, 974, 1291
HpaI GTTAAC 1 cut(s) 1291
HpaII CCGG 8 cut(s) 318, 324, 420, 486, 810, 815, 846, 891
HphI GGTGA 4 cut(s) 103, 860, 962, 1159
Hpy166II GTNNAC 3 cut(s) 699, 974, 1291
Hpy188I TCNGA 5 cut(s) 241, 331, 373, 457, 1209
Hpy188III TCNNGA 3 cut(s) 324, 1118, 1175
Hpy8I GTNNAC 3 cut(s) 699, 974, 1291
Hpy99I CGWCG 3 cut(s) 125, 128, 395
HpyAV CCTTC 3 cut(s) 213, 370, 412
HpyCH4III ACNGT 1 cut(s) 860
HpyCH4V TGCA 6 cut(s) 136, 342, 714, 789, 1066, 1256
HpyF3I CTNAG 5 cut(s) 576, 977, 1116, 1206, 1308
Hsp92I GRCGYC 1 cut(s) 1103
Hsp92II CATG 2 cut(s) 1019, 1178
HspAI GCGC 3 cut(s) 728, 1073, 1103
KasI GGCGCC 1 cut(s) 1102
Kpn2I TCCGGA 1 cut(s) 323
KroI GCCGGC 4 cut(s) 419, 485, 809, 890
KroNI GCCGGC 4 cut(s) 421, 487, 811, 892
KspAI GTTAAC 1 cut(s) 1291
Kzo9I GATC 3 cut(s) 326, 561, 996
LmnI GCTCC 5 cut(s) 173, 326, 507, 676, 1057
Lsp1109I GCAGC 5 cut(s) 1062, 1078, 1120, 1255, 1256
LweI GCATC 5 cut(s) 183, 447, 750, 1178, 1243
MaeI CTAG 1 cut(s) 797
MalI GATC 3 cut(s) 328, 563, 998
MbiI CCGCTC 2 cut(s) 168, 337
MboI GATC 3 cut(s) 326, 561, 996
MboII GAAGA 4 cut(s) 622, 974, 1051, 1272
MflI RGATCY 1 cut(s) 326
MhlI GDGCHC 3 cut(s) 504, 681, 990
MluCI AATT 5 cut(s) 631, 836, 981, 1111, 1273
Mly113I GGCGCC 1 cut(s) 1103
MlyI GAGTC 7 cut(s) 158, 230, 368, 381, 452, 1019, 1219
MmeI TCCRAC 3 cut(s) 264, 354, 396
MroI TCCGGA 1 cut(s) 323
MroNI GCCGGC 4 cut(s) 419, 485, 809, 890
MseI TTAA 2 cut(s) 1125, 1290
MslI CAYNNNNRTG 2 cut(s) 1051, 1145
MspA1I CMGCKG 2 cut(s) 673, 832
MspI CCGG 8 cut(s) 318, 324, 420, 486, 810, 815, 846, 891
MspR9I CCNGG 4 cut(s) 142, 683, 815, 1165
MvaI CCWGG 3 cut(s) 142, 683, 1165
MvnI CGCG 1 cut(s) 689
NaeI GCCGGC 4 cut(s) 421, 487, 811, 892
NarI GGCGCC 1 cut(s) 1103
NciI CCSGG 1 cut(s) 815
NdeII GATC 3 cut(s) 326, 561, 996
NgoMIV GCCGGC 4 cut(s) 419, 485, 809, 890
NlaIII CATG 2 cut(s) 1019, 1178
NlaIV GGNNCC 6 cut(s) 322, 328, 678, 794, 1104, 1185
NmeAIII GCCGAG 2 cut(s) 547, 1323
NspV TTCGAA 1 cut(s) 1283
PagI TCATGA 1 cut(s) 1174
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PdiI GCCGGC 4 cut(s) 421, 487, 811, 892
PfeI GAWTC 3 cut(s) 734, 842, 1042
PleI GAGTC 7 cut(s) 157, 230, 368, 381, 452, 1019, 1218
PluTI GGCGCC 1 cut(s) 1106
PpsI GAGTC 7 cut(s) 157, 230, 368, 381, 452, 1019, 1218
Psp6I CCWGG 3 cut(s) 140, 681, 1163
PspFI CCCAGC 1 cut(s) 875
PspGI CCWGG 3 cut(s) 140, 681, 1163
PspN4I GGNNCC 6 cut(s) 322, 328, 678, 794, 1104, 1185
PspPI GGNCC 6 cut(s) 4, 532, 708, 771, 807, 812
PsuI RGATCY 1 cut(s) 326
RigI GGCCGGCC 1 cut(s) 813
RseI CAYNNNNRTG 2 cut(s) 1051, 1145
SaqAI TTAA 2 cut(s) 1125, 1290
Sau3AI GATC 3 cut(s) 326, 561, 996
Sau96I GGNCC 6 cut(s) 4, 532, 708, 771, 807, 812
SchI GAGTC 7 cut(s) 158, 230, 368, 381, 452, 1019, 1219
ScrFI CCNGG 4 cut(s) 142, 683, 815, 1165
SduI GDGCHC 3 cut(s) 504, 681, 990
SfaNI GCATC 5 cut(s) 183, 447, 750, 1178, 1243
SfoI GGCGCC 1 cut(s) 1104
SfuI TTCGAA 1 cut(s) 1283
SinI GGWCC 2 cut(s) 4, 771
SmiMI CAYNNNNRTG 2 cut(s) 1051, 1145
Sse9I AATT 5 cut(s) 631, 836, 981, 1111, 1273
SspDI GGCGCC 1 cut(s) 1102
SspI AATATT 1 cut(s) 1083
SspMI CTAG 1 cut(s) 797
StyD4I CCNGG 4 cut(s) 140, 681, 813, 1163
StyI CCWWGG 2 cut(s) 528, 1248
TaaI ACNGT 1 cut(s) 860
TaqI TCGA 5 cut(s) 198, 273, 560, 601, 1283
TasI AATT 5 cut(s) 631, 836, 981, 1111, 1273
TauI GCSGC 6 cut(s) 287, 521, 670, 673, 835, 1189
TfiI GAWTC 3 cut(s) 734, 842, 1042
Tru1I TTAA 2 cut(s) 1125, 1290
Tru9I TTAA 2 cut(s) 1125, 1290
TscAI CASTG 3 cut(s) 865, 1009, 1263
TseI GCWGC 5 cut(s) 1066, 1075, 1133, 1244, 1268
TspDTI ATGAA 1 cut(s) 1266
TspGWI ACGGA 1 cut(s) 640
TspRI CASTG 3 cut(s) 865, 1009, 1263
VpaK11BI GGWCC 2 cut(s) 4, 771
XapI RAATTY 1 cut(s) 1111
XcmI CCANNNNNNNNNTGG 2 cut(s) 138, 1255
XspI CTAG 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.