Rh2CG233800

Hyccin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0014272
Physical Location & Seq
Forward (+)
23915207 .. 23916996
1790 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG233800.1

Sequence Viewer

Length: 1329 bp
ATGGACCTCTACCCAAATCACAGCACCAACTCCCCCACCTCCTCCGCCTCCTCCACCGCCACCAACCCAAACCCAAACCCCAGACCCACCCGCAACACCATCTCCTCCCTCACCGCCACCGACGACGACCCAATGCACTCCTGGTGGGAGTCCATCTCCAAAGCCCGCTCCCGCATCCACTCCCTCGCCACTCTCCTCGACCCTTCTCTCTCCTCCTCCCTCTCCTCCCTCGCCGACTCCGACCGCCCCGCCCTCTCTCTCCTCTCCTCCCTCGAAGCCTACGCCGCCGTTTCCGCCGTCCTCTCCGGCCCCCTCTCCGGCTCCGGATCCGACCCGCTCTGCAACTGGCTCTACGACACCTTCCTCTCCTCCGACCCCCATCTACGACTCGTCGTTTTCTCCTTCCTCCCTCTCCTCGCCGGCCTCTATCTCTCCCGCATCCACTCTCTCTCCTCTGACTCTCTTACCTCGCTTCCCTCCCTCGCCGGCTTTGAGGCCGTGCTCCTCGCTCTCTACGCCGCCGAGACCAAGGCCCGAAACGGCAAGCCCATTGTCGTTTCGATCCCAGACCTATCTCAGCCCTCTCTCTACCACGTTCCTCGAAGCCAGAAGACGACTAATCCCAACTCCAATTCCAGTAATATCCAATCCGTTGGGGTTTTGTCGCCGCCGCTGGAGCCCCAGGTCGCGGTCAAGTCAACCAAACGGGCCTGCATTGTGGGAGTTGCGCTGGATTCTTATTACAAGCACATTTCGCAGATGCCCACTTGGTCCAAGATGGATTTTTGCAGGTTCCTAGCTTCTTGGGCCGGCCCGGATTGCTCTTGTCAGCGGCAATTTGAATCCGGTGATAACCACAGTGAGCCAGAGATTGCTGGGTTCTTGGAGGCCGGTGATGGGAATGGGAGTGGTAATGAGAGTGAGATTGAGATTGAGGGTGATGTGGCGGAAGAAATGGGTCAACTAAGAATTGAGCAAAATGGGATCAGCAGTGGGGTCAGTCATGTGGAGGACTCAAAGGGGGCGAAGATTCCATTGCCGTGGGAGCTTTTGCAGCCAGCGCTGCGAATATTGGGGCATTGTTTGTTGGCGCCGTTGAATTCTCAGGAGGTTAAGGACGCTGCTTCCATTGCGGTGAGGAGATTGTATGCCAGGGCATCTCATGATTTGGTGCCGCAGGCGATTTTAGCTACTCAGAGTCTCATTCAGCTTGATAACAGGGGGCGTGAGGCAGCCAAGGATGCAGTGGCGGTTGCTGCTAATTCATCTTCGAATGTTAACACGCCGAGCAAAGCTAAGAAACCTGAAATCCTTTTGGTCTCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hyccin

Protein Analysis

442

Amino Acids

47.22

Weight (kDa)

5.68

Isoelectric Point (pI)

53.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hyccin PF09790 79 - 404 4.5e-60 Hyccin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64090
fragaria_vesca FvH4_1g19990
malus_domestica MD05G1042400.v1.1 MD10G1049000.v1.1
prunus_persica Prupe.8G059200_v2.0.a1
pyrus_communis pycom05g03500
rosa_chinensis RchiOBHm_Chr2g0111931
rosa_laevigata RLG00000017939 RLG00000034552
rosa_multiflora Rmu_sc0005679.1_g000010
rosa_roxburghii Rroxscaffold_2G00131980
rosa_rugosa Rorug02G0177300
rosa_samantha Rh2AG229700 Rh2BG242400 Rh2CG233800 Rh2DG237400
rosa_wichuraiana Rw2G017770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 780
AccB1I GGYRCC 2 cut(s) 1090, 1171
AccBSI CCGCTC 2 cut(s) 168, 337
AccII CGCG 1 cut(s) 689
AccIII TCCGGA 1 cut(s) 323
AclWI GGATC 4 cut(s) 321, 334, 556, 992
AcsI RAATTY 1 cut(s) 1099
AcyI GRCGYC 1 cut(s) 1091
AfeI AGCGCT 1 cut(s) 1062
AfiI CCNNNNNNNGG 3 cut(s) 317, 688, 897
AgsI TTSAA 2 cut(s) 842, 1099
AjnI CCWGG 3 cut(s) 140, 681, 1151
AluBI AGCT 5 cut(s) 800, 1048, 1190, 1210, 1295
AluI AGCT 5 cut(s) 800, 1048, 1190, 1210, 1295
Alw21I GWGCWC 1 cut(s) 504
Alw26I GTCTC 3 cut(s) 518, 1205, 1324
AlwI GGATC 4 cut(s) 321, 334, 556, 992
Aor13HI TCCGGA 1 cut(s) 323
Aor51HI AGCGCT 1 cut(s) 1062
AoxI GGCC 8 cut(s) 307, 421, 495, 531, 708, 807, 811, 888
ApeKI GCWGC 5 cut(s) 1054, 1063, 1121, 1232, 1256
ApoI RAATTY 1 cut(s) 1099
AspLEI GCGC 3 cut(s) 730, 1063, 1093
AspS9I GGNCC 7 cut(s) 4, 308, 532, 708, 771, 807, 812
AsuC2I CCSGG 1 cut(s) 815
AsuHPI GGTGA 5 cut(s) 103, 860, 905, 950, 1147
AsuII TTCGAA 1 cut(s) 1271
AvaII GGWCC 2 cut(s) 4, 771
BamHI GGATCC 1 cut(s) 326
BanI GGYRCC 2 cut(s) 1090, 1171
BanII GRGCYC 1 cut(s) 681
BbsI GAAGAC 1 cut(s) 617
Bbv12I GWGCWC 1 cut(s) 504
BbvI GCAGC 5 cut(s) 1050, 1066, 1108, 1243, 1244
BccI CCATC 5 cut(s) 107, 161, 387, 772, 890
BceAI ACGGC 6 cut(s) 272, 281, 482, 556, 1024, 1078
BciT130I CCWGG 3 cut(s) 142, 683, 1153
BcnI CCSGG 1 cut(s) 815
BcoDI GTCTC 3 cut(s) 518, 1205, 1324
BfaI CTAG 1 cut(s) 797
BfoI RGCGCY 2 cut(s) 1064, 1094
BfuAI ACCTGC 1 cut(s) 780
Bme1390I CCNGG 4 cut(s) 142, 683, 815, 1153
Bme18I GGWCC 2 cut(s) 4, 771
BmgT120I GGNCC 7 cut(s) 4, 308, 532, 708, 771, 807, 812
BmiI GGNNCC 7 cut(s) 310, 322, 328, 678, 794, 1092, 1173
BmrFI CCNGG 4 cut(s) 142, 683, 815, 1153
BmsI GCATC 5 cut(s) 183, 447, 750, 1166, 1231
BpiI GAAGAC 1 cut(s) 617
BplI GAGNNNNNCTC 2 cut(s) 140, 172
BpmI CTGGAG 1 cut(s) 695
Bpu14I TTCGAA 1 cut(s) 1271
BpuMI CCSGG 1 cut(s) 815
BsaHI GRCGYC 1 cut(s) 1091
BsaI GGTCTC 2 cut(s) 518, 1324
BsaJI CCNNGG 5 cut(s) 528, 681, 1040, 1152, 1236
BsaWI WCCGGW 2 cut(s) 323, 845
BsaXI ACNNNNNCTCC 4 cut(s) 86, 116, 434, 464
Bsc4I CCNNNNNNNGG 3 cut(s) 317, 688, 897
Bse118I RCCGGY 4 cut(s) 419, 485, 809, 890
Bse1I ACTGG 2 cut(s) 350, 636
Bse3DI GCAATG 2 cut(s) 1034, 1128
BseAI TCCGGA 1 cut(s) 323
BseBI CCWGG 3 cut(s) 142, 683, 1153
BseDI CCNNGG 5 cut(s) 528, 681, 1040, 1152, 1236
BseGI GGATG 3 cut(s) 174, 438, 1246
BseLI CCNNNNNNNGG 3 cut(s) 317, 688, 897
BseMI GCAATG 2 cut(s) 1034, 1128
BseMII CTCAG 3 cut(s) 590, 1118, 1208
BseNI ACTGG 2 cut(s) 350, 636
BseXI GCAGC 5 cut(s) 1050, 1066, 1108, 1243, 1244
BseYI CCCAGC 1 cut(s) 875
Bsh1236I CGCG 1 cut(s) 689
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 8 cut(s) 309, 423, 497, 533, 710, 809, 813, 890
BshNI GGYRCC 2 cut(s) 1090, 1171
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 1 cut(s) 504
BsiSI CCGG 9 cut(s) 306, 318, 324, 420, 486, 810, 815, 846, 891
BslI CCNNNNNNNGG 3 cut(s) 317, 688, 897
BsmAI GTCTC 3 cut(s) 518, 1205, 1324
BsnI GGCC 8 cut(s) 309, 423, 497, 533, 710, 809, 813, 890
Bso31I GGTCTC 2 cut(s) 518, 1324
Bsp119I TTCGAA 1 cut(s) 1271
Bsp1286I GDGCHC 2 cut(s) 504, 681
Bsp13I TCCGGA 1 cut(s) 323
Bsp143I GATC 3 cut(s) 326, 561, 984
BspANI GGCC 8 cut(s) 309, 423, 497, 533, 710, 809, 813, 890
BspCNI CTCAG 3 cut(s) 589, 1117, 1207
BspEI TCCGGA 1 cut(s) 323
BspFNI CGCG 1 cut(s) 689
BspHI TCATGA 1 cut(s) 1162
BspLI GGNNCC 7 cut(s) 310, 322, 328, 678, 794, 1092, 1173
BspMI ACCTGC 1 cut(s) 780
BspPI GGATC 4 cut(s) 321, 334, 556, 992
BspT104I TTCGAA 1 cut(s) 1271
BspT107I GGYRCC 2 cut(s) 1090, 1171
BspTNI GGTCTC 2 cut(s) 518, 1324
BsrBI CCGCTC 2 cut(s) 168, 337
BsrDI GCAATG 2 cut(s) 1034, 1128
BsrFI RCCGGY 4 cut(s) 419, 485, 809, 890
BsrI ACTGG 2 cut(s) 350, 636
BssAI RCCGGY 4 cut(s) 419, 485, 809, 890
BssECI CCNNGG 5 cut(s) 528, 681, 1040, 1152, 1236
BssMI GATC 3 cut(s) 326, 561, 984
BssNI GRCGYC 1 cut(s) 1091
BssT1I CCWWGG 2 cut(s) 528, 1236
Bst2UI CCWGG 3 cut(s) 142, 683, 1153
Bst4CI ACNGT 1 cut(s) 860
BstACI GRCGYC 1 cut(s) 1091
BstBI TTCGAA 1 cut(s) 1271
BstC8I GCNNGC 8 cut(s) 166, 421, 487, 545, 712, 811, 1059, 1179
BstDEI CTNAG 5 cut(s) 576, 965, 1104, 1194, 1296
BstDSI CCRYGG 1 cut(s) 1040
BstF5I GGATG 3 cut(s) 174, 438, 1246
BstFNI CGCG 1 cut(s) 689
BstH2I RGCGCY 2 cut(s) 1064, 1094
BstHHI GCGC 3 cut(s) 730, 1063, 1093
BstKTI GATC 3 cut(s) 329, 564, 987
BstMAI GTCTC 3 cut(s) 518, 1205, 1324
BstMBI GATC 3 cut(s) 326, 561, 984
BstMCI CGRYCG 1 cut(s) 244
BstNI CCWGG 3 cut(s) 142, 683, 1153
BstSCI CCNGG 4 cut(s) 140, 681, 813, 1151
BstUI CGCG 1 cut(s) 689
BstV1I GCAGC 5 cut(s) 1050, 1066, 1108, 1243, 1244
BstV2I GAAGAC 1 cut(s) 617
BstX2I RGATCY 1 cut(s) 326
BstXI CCANNNNNNTGG 2 cut(s) 653, 1041
BstYI RGATCY 1 cut(s) 326
BsuRI GGCC 8 cut(s) 309, 423, 497, 533, 710, 809, 813, 890
BtgI CCRYGG 1 cut(s) 1040
BtsCI GGATG 3 cut(s) 174, 438, 1246
BtsI GCAGTG 2 cut(s) 997, 1251
BtsIMutI CAGTG 3 cut(s) 865, 997, 1251
BveI ACCTGC 1 cut(s) 780
Cac8I GCNNGC 8 cut(s) 166, 421, 487, 545, 712, 811, 1059, 1179
CciI TCATGA 1 cut(s) 1162
CfoI GCGC 3 cut(s) 730, 1063, 1093
Cfr10I RCCGGY 4 cut(s) 419, 485, 809, 890
Cfr13I GGNCC 7 cut(s) 4, 308, 532, 708, 771, 807, 812
CseI GACGC 1 cut(s) 1127
CviAII CATG 2 cut(s) 1004, 1163
DdeI CTNAG 5 cut(s) 576, 965, 1104, 1194, 1296
DinI GGCGCC 1 cut(s) 1092
DpnI GATC 3 cut(s) 328, 563, 986
DpnII GATC 3 cut(s) 326, 561, 984
EciI GGCGGA 3 cut(s) 34, 283, 962
Eco130I CCWWGG 2 cut(s) 528, 1236
Eco24I GRGCYC 1 cut(s) 681
Eco31I GGTCTC 2 cut(s) 518, 1324
Eco47I GGWCC 2 cut(s) 4, 771
Eco47III AGCGCT 1 cut(s) 1062
EcoRI GAATTC 1 cut(s) 1099
EcoRII CCWGG 3 cut(s) 140, 681, 1151
EcoT14I CCWWGG 2 cut(s) 528, 1236
EcoT38I GRGCYC 1 cut(s) 681
EgeI GGCGCC 1 cut(s) 1092
EheI GGCGCC 1 cut(s) 1092
ErhI CCWWGG 2 cut(s) 528, 1236
FaeI CATG 2 cut(s) 1007, 1166
FaiI YATR 3 cut(s) 1005, 1149, 1164
FatI CATG 2 cut(s) 1003, 1162
FauI CCCGC 6 cut(s) 98, 173, 179, 256, 342, 443
FokI GGATG 3 cut(s) 161, 425, 1253
FriOI GRGCYC 1 cut(s) 681
FseI GGCCGGCC 1 cut(s) 813
FspBI CTAG 1 cut(s) 797
GlaI GCGC 3 cut(s) 729, 1062, 1092
GsaI CCCAGC 1 cut(s) 879
GsuI CTGGAG 1 cut(s) 695
HaeII RGCGCY 2 cut(s) 1064, 1094
HaeIII GGCC 8 cut(s) 309, 423, 497, 533, 710, 809, 813, 890
HapII CCGG 9 cut(s) 306, 318, 324, 420, 486, 810, 815, 846, 891
HgaI GACGC 1 cut(s) 1127
HhaI GCGC 3 cut(s) 730, 1063, 1093
Hin1I GRCGYC 1 cut(s) 1091
Hin1II CATG 2 cut(s) 1007, 1166
Hin6I GCGC 3 cut(s) 728, 1061, 1091
HinP1I GCGC 3 cut(s) 728, 1061, 1091
HincII GTYRAC 3 cut(s) 699, 962, 1279
HindII GTYRAC 3 cut(s) 699, 962, 1279
HinfI GANTC 9 cut(s) 149, 236, 387, 458, 734, 842, 1013, 1030, 1198
HpaI GTTAAC 1 cut(s) 1279
HpaII CCGG 9 cut(s) 306, 318, 324, 420, 486, 810, 815, 846, 891
HphI GGTGA 5 cut(s) 103, 860, 905, 950, 1147
Hpy166II GTNNAC 3 cut(s) 699, 962, 1279
Hpy188I TCNGA 5 cut(s) 241, 331, 373, 457, 1197
Hpy188III TCNNGA 3 cut(s) 324, 1106, 1163
Hpy8I GTNNAC 3 cut(s) 699, 962, 1279
Hpy99I CGWCG 3 cut(s) 125, 128, 395
HpyAV CCTTC 3 cut(s) 213, 370, 412
HpyCH4III ACNGT 1 cut(s) 860
HpyCH4IV ACGT 1 cut(s) 594
HpyCH4V TGCA 6 cut(s) 136, 342, 714, 789, 1054, 1244
HpyF3I CTNAG 5 cut(s) 576, 965, 1104, 1194, 1296
HpySE526I ACGT 1 cut(s) 594
Hsp92I GRCGYC 1 cut(s) 1091
Hsp92II CATG 2 cut(s) 1007, 1166
HspAI GCGC 3 cut(s) 728, 1061, 1091
KasI GGCGCC 1 cut(s) 1090
Kpn2I TCCGGA 1 cut(s) 323
KroI GCCGGC 3 cut(s) 419, 485, 809
KroNI GCCGGC 3 cut(s) 421, 487, 811
KspAI GTTAAC 1 cut(s) 1279
Kzo9I GATC 3 cut(s) 326, 561, 984
LmnI GCTCC 5 cut(s) 173, 326, 507, 676, 1045
Lsp1109I GCAGC 5 cut(s) 1050, 1066, 1108, 1243, 1244
LweI GCATC 5 cut(s) 183, 447, 750, 1166, 1231
MaeI CTAG 1 cut(s) 797
MaeII ACGT 1 cut(s) 594
MalI GATC 3 cut(s) 328, 563, 986
MbiI CCGCTC 2 cut(s) 168, 337
MboI GATC 3 cut(s) 326, 561, 984
MboII GAAGA 4 cut(s) 622, 962, 1039, 1260
MflI RGATCY 1 cut(s) 326
MhlI GDGCHC 2 cut(s) 504, 681
MluCI AATT 5 cut(s) 631, 836, 969, 1099, 1261
Mly113I GGCGCC 1 cut(s) 1091
MlyI GAGTC 6 cut(s) 158, 230, 381, 452, 1007, 1207
MmeI TCCRAC 3 cut(s) 264, 354, 396
MroI TCCGGA 1 cut(s) 323
MroNI GCCGGC 3 cut(s) 419, 485, 809
MseI TTAA 2 cut(s) 1113, 1278
MslI CAYNNNNRTG 2 cut(s) 1039, 1133
MspA1I CMGCKG 2 cut(s) 673, 832
MspI CCGG 9 cut(s) 306, 318, 324, 420, 486, 810, 815, 846, 891
MspR9I CCNGG 4 cut(s) 142, 683, 815, 1153
MvaI CCWGG 3 cut(s) 142, 683, 1153
MvnI CGCG 1 cut(s) 689
NaeI GCCGGC 3 cut(s) 421, 487, 811
NarI GGCGCC 1 cut(s) 1091
NciI CCSGG 1 cut(s) 815
NdeII GATC 3 cut(s) 326, 561, 984
NgoMIV GCCGGC 3 cut(s) 419, 485, 809
NlaIII CATG 2 cut(s) 1007, 1166
NlaIV GGNNCC 7 cut(s) 310, 322, 328, 678, 794, 1092, 1173
NmeAIII GCCGAG 2 cut(s) 547, 1311
NspV TTCGAA 1 cut(s) 1271
PagI TCATGA 1 cut(s) 1162
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PdiI GCCGGC 3 cut(s) 421, 487, 811
PfeI GAWTC 3 cut(s) 734, 842, 1030
PleI GAGTC 6 cut(s) 157, 230, 381, 452, 1007, 1206
PluTI GGCGCC 1 cut(s) 1094
PpsI GAGTC 6 cut(s) 157, 230, 381, 452, 1007, 1206
Psp6I CCWGG 3 cut(s) 140, 681, 1151
PspFI CCCAGC 1 cut(s) 875
PspGI CCWGG 3 cut(s) 140, 681, 1151
PspN4I GGNNCC 7 cut(s) 310, 322, 328, 678, 794, 1092, 1173
PspPI GGNCC 7 cut(s) 4, 308, 532, 708, 771, 807, 812
PsuI RGATCY 1 cut(s) 326
RigI GGCCGGCC 1 cut(s) 813
RseI CAYNNNNRTG 2 cut(s) 1039, 1133
SaqAI TTAA 2 cut(s) 1113, 1278
Sau3AI GATC 3 cut(s) 326, 561, 984
Sau96I GGNCC 7 cut(s) 4, 308, 532, 708, 771, 807, 812
SchI GAGTC 6 cut(s) 158, 230, 381, 452, 1007, 1207
ScrFI CCNGG 4 cut(s) 142, 683, 815, 1153
SduI GDGCHC 2 cut(s) 504, 681
SfaNI GCATC 5 cut(s) 183, 447, 750, 1166, 1231
SfoI GGCGCC 1 cut(s) 1092
SfuI TTCGAA 1 cut(s) 1271
SinI GGWCC 2 cut(s) 4, 771
SmiMI CAYNNNNRTG 2 cut(s) 1039, 1133
Sse9I AATT 5 cut(s) 631, 836, 969, 1099, 1261
SspDI GGCGCC 1 cut(s) 1090
SspI AATATT 1 cut(s) 1071
SspMI CTAG 1 cut(s) 797
StyD4I CCNGG 4 cut(s) 140, 681, 813, 1151
StyI CCWWGG 2 cut(s) 528, 1236
TaaI ACNGT 1 cut(s) 860
TaiI ACGT 1 cut(s) 597
TaqI TCGA 5 cut(s) 198, 273, 560, 601, 1271
TasI AATT 5 cut(s) 631, 836, 969, 1099, 1261
TauI GCSGC 6 cut(s) 287, 521, 670, 673, 835, 1177
TfiI GAWTC 3 cut(s) 734, 842, 1030
Tru1I TTAA 2 cut(s) 1113, 1278
Tru9I TTAA 2 cut(s) 1113, 1278
TscAI CASTG 3 cut(s) 865, 997, 1251
TseI GCWGC 5 cut(s) 1054, 1063, 1121, 1232, 1256
TspDTI ATGAA 1 cut(s) 1254
TspGWI ACGGA 1 cut(s) 640
TspRI CASTG 3 cut(s) 865, 997, 1251
VpaK11BI GGWCC 2 cut(s) 4, 771
XapI RAATTY 1 cut(s) 1099
XcmI CCANNNNNNNNNTGG 2 cut(s) 138, 1243
XspI CTAG 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.