Prupe.8G059200_v2.0.a1

Hyccin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0014272
Physical Location & Seq
Reverse (-)
7647376 .. 7649339
1964 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G059200.1

Sequence Viewer

Length: 1290 bp
ATGGACTTCCACCGAAACCACCACAGCACCACCTCCCCCACATCCTCTGCTTCCTCCACCACCACCACCACCACCACCCCCATTCCCAACCCTAACCCTAATCCTTCCTCCAACTGCATCTCTACCGACGACCCAATGCACTCCTGGTGGGAGTCCATCTCCAAAGCCCGCTCTCGCATCCACTCCTTGTCCTCTCTCCTCCCCCCTTCTCTCTCCTCCTGTCTCTGCTCCCTCGCCGACTCCGACCGCCCTGCCCTCTCCCTTCTGTCCTCCCTTGAGTCCTACGACGCCGTTTCTTCCGCCCTATTCTCCCCTCTCTCCGGTTCAGGATCCGACCCCCTCTGCCACTGGCTCTACGATACCTTCCTCTCCTCTGACCCCCATCTACGACTCGTCGTTTTCTCCTTCCTCCCTGTCCTCTCTGGCCTCTACCTCTCCCGAGTCCATTCTCTCTCCTCTGACTCTCCCTCTCTTCCCTCCCTCGCCGGCTTCGAAGCTGTTCTCCTCGCCCTCTACGCCGCCGAGACCAAGGCTCGAAACGGCAAGCCTGTTGTCGTTTCGATCCCAGATCTCTCTCAGCCCTCTCTCTACCACACCCCTCGAGTTCACAAGCCCAACCCCCTCGCTCCCGCCATTTCCCCCCACCAGTCAATTGGGGTTTTGTCGCCGCCTCTGGAGCCCCAGATCGCCGTCAAGTCCACCAAACGTGCCTGCATTGTCGGCGTTGCCCTCGATTCCTATTACAAGCACATTTCCCAGATGCCCACTTGGTCCAAGATTGACTTTTGTCGATTCCTAGCTTCTTGGGCCGGCCAGGATTGCCATTGTCTGCACCAAGTCAGTGACGATGACACCAATGAGCCCGACATTACTCCGGCATTGTTCTTGGAGGGCGGCAATGAGATTGACGATGTGACTGAAGAAATGGATCAGTTAATGAGATTTGAGAAGAAAAATGTTTGCAATGGTGTAGTTTTGGAGTCAAAGGGGTCAATAATTCCATTACCTTGGGAGCTTTTGCAGCCAGCGTTGCGAATAGTGGGGCATTGTCTGTTGGCCCCATTGAATTCTCAGGACATTAAGGACGCGGCTTCTGTTGCAGTGAAGCGGCTGTATGCTCGGGCATCTCATGATTTGGTGCCACAGGCGATTTTAGCTACTCAGAGCCTAATTCAGCTAGATAAGAGGGCACGGGAGTCTGCCAAGGCTACTGCTGTTGCTAATTCGTCTTCAAACGCTAACACCCCAAGCAAAGCTAAAAACCCCGAAGTGCTTTTGGTCTCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hyccin

Protein Analysis

430

Amino Acids

46.37

Weight (kDa)

6.23

Isoelectric Point (pI)

53.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64090
fragaria_vesca FvH4_1g19990
malus_domestica MD05G1042400.v1.1 MD10G1049000.v1.1
prunus_persica Prupe.8G059200_v2.0.a1
pyrus_communis pycom05g03500
rosa_chinensis RchiOBHm_Chr2g0111931
rosa_laevigata RLG00000017939 RLG00000034552
rosa_multiflora Rmu_sc0005679.1_g000010
rosa_roxburghii Rroxscaffold_2G00131980
rosa_rugosa Rorug02G0177300
rosa_samantha Rh2AG229700 Rh2BG242400 Rh2CG233800 Rh2DG237400
rosa_wichuraiana Rw2G017770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1138
AccBSI CCGCTC 1 cut(s) 171
AccII CGCG 1 cut(s) 1088
AciI CCGC 9 cut(s) 169, 247, 300, 519, 630, 668, 894, 1088, 1108
AclWI GGATC 4 cut(s) 324, 337, 556, 936
AcoI YGGCCR 1 cut(s) 811
AcsI RAATTY 1 cut(s) 1066
AcuI CTGAAG 1 cut(s) 939
AcyI GRCGYC 1 cut(s) 288
AfiI CCNNNNNNNGG 1 cut(s) 320
AgsI TTSAA 2 cut(s) 1066, 1233
AjnI CCWGG 2 cut(s) 143, 813
AluBI AGCT 6 cut(s) 497, 800, 1015, 1157, 1177, 1256
AluI AGCT 6 cut(s) 497, 800, 1015, 1157, 1177, 1256
Alw26I GTCTC 3 cut(s) 227, 518, 1285
AlwI GGATC 4 cut(s) 324, 337, 556, 936
Ama87I CYCGRG 3 cut(s) 438, 600, 1119
AoxI GGCC 4 cut(s) 424, 807, 811, 1056
ApeKI GCWGC 1 cut(s) 1021
ApoI RAATTY 1 cut(s) 1066
Asp700I GAANNNNTTC 1 cut(s) 498
AspS9I GGNCC 3 cut(s) 771, 807, 1057
AsuII TTCGAA 1 cut(s) 492
AvaI CYCGRG 3 cut(s) 438, 600, 1119
AvaII GGWCC 1 cut(s) 771
BaeGI GKGCMC 1 cut(s) 1192
BamHI GGATCC 1 cut(s) 329
BanI GGYRCC 1 cut(s) 1138
BanII GRGCYC 2 cut(s) 681, 864
BbsI GAAGAC 1 cut(s) 1221
BbvI GCAGC 1 cut(s) 1033
BccI CCATC 2 cut(s) 164, 390
BceAI ACGGC 3 cut(s) 275, 556, 674
BcgI CGANNNNNNTGC 2 cut(s) 233, 267
BciT130I CCWGG 2 cut(s) 145, 815
BcoDI GTCTC 3 cut(s) 227, 518, 1285
BfaI CTAG 2 cut(s) 797, 1178
BglII AGATCT 1 cut(s) 568
BisI GCNGC 6 cut(s) 519, 668, 895, 1022, 1089, 1109
BlsI GCNGC 6 cut(s) 520, 669, 896, 1023, 1090, 1110
Bme1390I CCNGG 2 cut(s) 145, 815
Bme18I GGWCC 1 cut(s) 771
BmeT110I CYCGRG 3 cut(s) 438, 600, 1119
BmgT120I GGNCC 3 cut(s) 771, 807, 1057
BmiI GGNNCC 4 cut(s) 331, 678, 1059, 1140
BmrFI CCNGG 2 cut(s) 145, 815
BmsI GCATC 4 cut(s) 126, 186, 750, 1133
BoxI GACNNNNGTC 1 cut(s) 786
BpiI GAAGAC 1 cut(s) 1221
BplI GAGNNNNNCTC 2 cut(s) 143, 175
BpmI CTGGAG 1 cut(s) 695
Bpu14I TTCGAA 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 296
BsaHI GRCGYC 1 cut(s) 288
BsaI GGTCTC 2 cut(s) 518, 1285
BsaJI CCNNGG 3 cut(s) 528, 1007, 1203
BsaWI WCCGGW 1 cut(s) 320
Bsc4I CCNNNNNNNGG 1 cut(s) 320
Bse118I RCCGGY 2 cut(s) 485, 809
Bse1I ACTGG 2 cut(s) 353, 646
Bse3DI GCAATG 2 cut(s) 904, 970
BseBI CCWGG 2 cut(s) 145, 815
BseDI CCNNGG 3 cut(s) 528, 1007, 1203
BseGI GGATG 2 cut(s) 41, 177
BseLI CCNNNNNNNGG 1 cut(s) 320
BseMI GCAATG 2 cut(s) 904, 970
BseMII CTCAG 3 cut(s) 590, 1085, 1175
BseNI ACTGG 2 cut(s) 353, 646
BseRI GAGGAG 5 cut(s) 188, 205, 361, 445, 494
BseSI GKGCMC 1 cut(s) 1192
BseXI GCAGC 1 cut(s) 1033
BsgI GTGCAG 1 cut(s) 815
Bsh1236I CGCG 1 cut(s) 1088
Bsh1285I CGRYCG 1 cut(s) 247
BshFI GGCC 4 cut(s) 426, 809, 813, 1058
BshNI GGYRCC 1 cut(s) 1138
BsiEI CGRYCG 1 cut(s) 247
BsiHKCI CYCGRG 3 cut(s) 438, 600, 1119
BsiSI CCGG 4 cut(s) 321, 486, 810, 875
BslI CCNNNNNNNGG 1 cut(s) 320
BsmAI GTCTC 3 cut(s) 227, 518, 1285
BsnI GGCC 4 cut(s) 426, 809, 813, 1058
Bso31I GGTCTC 2 cut(s) 518, 1285
BsoBI CYCGRG 3 cut(s) 438, 600, 1119
Bsp119I TTCGAA 1 cut(s) 492
Bsp1286I GDGCHC 3 cut(s) 681, 864, 1192
Bsp143I GATC 5 cut(s) 329, 561, 568, 684, 928
BspACI CCGC 9 cut(s) 169, 247, 300, 519, 630, 668, 894, 1088, 1108
BspANI GGCC 4 cut(s) 426, 809, 813, 1058
BspCNI CTCAG 3 cut(s) 589, 1084, 1174
BspFNI CGCG 1 cut(s) 1088
BspHI TCATGA 1 cut(s) 1129
BspLI GGNNCC 4 cut(s) 331, 678, 1059, 1140
BspPI GGATC 4 cut(s) 324, 337, 556, 936
BspT104I TTCGAA 1 cut(s) 492
BspT107I GGYRCC 1 cut(s) 1138
BspTNI GGTCTC 2 cut(s) 518, 1285
BsrBI CCGCTC 1 cut(s) 171
BsrDI GCAATG 2 cut(s) 904, 970
BsrFI RCCGGY 2 cut(s) 485, 809
BsrI ACTGG 2 cut(s) 353, 646
BssAI RCCGGY 2 cut(s) 485, 809
BssECI CCNNGG 3 cut(s) 528, 1007, 1203
BssMI GATC 5 cut(s) 329, 561, 568, 684, 928
BssNI GRCGYC 1 cut(s) 288
BssT1I CCWWGG 3 cut(s) 528, 1007, 1203
Bst2UI CCWGG 2 cut(s) 145, 815
Bst6I CTCTTC 1 cut(s) 477
BstACI GRCGYC 1 cut(s) 288
BstBI TTCGAA 1 cut(s) 492
BstC8I GCNNGC 6 cut(s) 169, 487, 545, 712, 811, 1026
BstDEI CTNAG 3 cut(s) 576, 1071, 1161
BstF5I GGATG 2 cut(s) 41, 177
BstFNI CGCG 1 cut(s) 1088
BstKTI GATC 5 cut(s) 332, 564, 571, 687, 931
BstMAI GTCTC 3 cut(s) 227, 518, 1285
BstMBI GATC 5 cut(s) 329, 561, 568, 684, 928
BstMCI CGRYCG 1 cut(s) 247
BstMWI GCNNNNNNNGC 9 cut(s) 515, 676, 720, 806, 819, 1021, 1030, 1097, 1154
BstNI CCWGG 2 cut(s) 145, 815
BstPAI GACNNNNGTC 1 cut(s) 786
BstSCI CCNGG 2 cut(s) 143, 813
BstSLI GKGCMC 1 cut(s) 1192
BstUI CGCG 1 cut(s) 1088
BstV1I GCAGC 1 cut(s) 1033
BstV2I GAAGAC 1 cut(s) 1221
BstX2I RGATCY 2 cut(s) 329, 568
BstXI CCANNNNNNTGG 2 cut(s) 653, 1008
BstYI RGATCY 2 cut(s) 329, 568
BsuRI GGCC 4 cut(s) 426, 809, 813, 1058
BtsCI GGATG 2 cut(s) 41, 177
BtsI GCAGTG 1 cut(s) 1107
BtsIMutI CAGTG 3 cut(s) 346, 847, 1107
Cac8I GCNNGC 6 cut(s) 169, 487, 545, 712, 811, 1026
CciI TCATGA 1 cut(s) 1129
Cfr10I RCCGGY 2 cut(s) 485, 809
Cfr13I GGNCC 3 cut(s) 771, 807, 1057
CseI GACGC 2 cut(s) 296, 1094
CviAII CATG 1 cut(s) 1130
DdeI CTNAG 3 cut(s) 576, 1071, 1161
DpnI GATC 5 cut(s) 331, 563, 570, 686, 930
DpnII GATC 5 cut(s) 329, 561, 568, 684, 928
EaeI YGGCCR 1 cut(s) 811
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
EciI GGCGGA 1 cut(s) 289
Eco130I CCWWGG 3 cut(s) 528, 1007, 1203
Eco24I GRGCYC 2 cut(s) 681, 864
Eco31I GGTCTC 2 cut(s) 518, 1285
Eco47I GGWCC 1 cut(s) 771
Eco57I CTGAAG 1 cut(s) 939
Eco88I CYCGRG 3 cut(s) 438, 600, 1119
EcoRI GAATTC 1 cut(s) 1066
EcoRII CCWGG 2 cut(s) 143, 813
EcoT14I CCWWGG 3 cut(s) 528, 1007, 1203
EcoT38I GRGCYC 2 cut(s) 681, 864
ErhI CCWWGG 3 cut(s) 528, 1007, 1203
FaeI CATG 1 cut(s) 1133
FaiI YATR 2 cut(s) 1116, 1131
FalI AAGNNNNNCTT 2 cut(s) 767, 799
FatI CATG 1 cut(s) 1129
FauI CCCGC 2 cut(s) 176, 637
Fnu4HI GCNGC 6 cut(s) 519, 668, 895, 1022, 1089, 1109
FokI GGATG 2 cut(s) 28, 164
FriOI GRGCYC 2 cut(s) 681, 864
FseI GGCCGGCC 1 cut(s) 813
Fsp4HI GCNGC 6 cut(s) 519, 668, 895, 1022, 1089, 1109
FspBI CTAG 2 cut(s) 797, 1178
GluI GCNGC 6 cut(s) 519, 668, 895, 1022, 1089, 1109
GsuI CTGGAG 1 cut(s) 695
HaeIII GGCC 4 cut(s) 426, 809, 813, 1058
HapII CCGG 4 cut(s) 321, 486, 810, 875
HgaI GACGC 2 cut(s) 296, 1094
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 1 cut(s) 1133
HpaII CCGG 4 cut(s) 321, 486, 810, 875
Hpy166II GTNNAC 2 cut(s) 607, 699
Hpy188I TCNGA 5 cut(s) 244, 334, 376, 460, 1164
Hpy188III TCNNGA 5 cut(s) 327, 438, 674, 1073, 1130
Hpy8I GTNNAC 2 cut(s) 607, 699
Hpy99I CGWCG 3 cut(s) 131, 290, 398
HpyAV CCTTC 5 cut(s) 114, 216, 272, 373, 415
HpyCH4IV ACGT 1 cut(s) 706
HpyCH4V TGCA 7 cut(s) 117, 139, 714, 832, 963, 1021, 1100
HpyF10VI GCNNNNNNNGC 9 cut(s) 515, 676, 720, 806, 819, 1021, 1030, 1097, 1154
HpyF3I CTNAG 3 cut(s) 576, 1071, 1161
HpySE526I ACGT 1 cut(s) 706
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 1 cut(s) 1133
KroI GCCGGC 2 cut(s) 485, 809
KroNI GCCGGC 2 cut(s) 487, 811
Kzo9I GATC 5 cut(s) 329, 561, 568, 684, 928
LmnI GCTCC 4 cut(s) 233, 631, 676, 1012
Lsp1109I GCAGC 1 cut(s) 1033
LweI GCATC 4 cut(s) 126, 186, 750, 1133
MaeI CTAG 2 cut(s) 797, 1178
MaeII ACGT 1 cut(s) 706
MaeIII GTNAC 2 cut(s) 842, 913
MalI GATC 5 cut(s) 331, 563, 570, 686, 930
MbiI CCGCTC 1 cut(s) 171
MboI GATC 5 cut(s) 329, 561, 568, 684, 928
MboII GAAGA 5 cut(s) 288, 464, 932, 961, 1221
MfeI CAATTG 1 cut(s) 651
MflI RGATCY 2 cut(s) 329, 568
MhlI GDGCHC 3 cut(s) 681, 864, 1192
MluCI AATT 5 cut(s) 651, 996, 1066, 1170, 1222
MlyI GAGTC 8 cut(s) 161, 233, 287, 384, 450, 455, 989, 1205
MmeI TCCRAC 3 cut(s) 135, 267, 357
MroNI GCCGGC 2 cut(s) 485, 809
MroXI GAANNNNTTC 1 cut(s) 498
MseI TTAA 2 cut(s) 935, 1080
MspI CCGG 4 cut(s) 321, 486, 810, 875
MspR9I CCNGG 2 cut(s) 145, 815
MunI CAATTG 1 cut(s) 651
MvaI CCWGG 2 cut(s) 145, 815
MvnI CGCG 1 cut(s) 1088
MwoI GCNNNNNNNGC 9 cut(s) 515, 676, 720, 806, 819, 1021, 1030, 1097, 1154
NaeI GCCGGC 2 cut(s) 487, 811
NdeII GATC 5 cut(s) 329, 561, 568, 684, 928
NgoMIV GCCGGC 2 cut(s) 485, 809
NlaIII CATG 1 cut(s) 1133
NlaIV GGNNCC 4 cut(s) 331, 678, 1059, 1140
NmeAIII GCCGAG 1 cut(s) 547
NmuCI GTSAC 2 cut(s) 842, 913
NspV TTCGAA 1 cut(s) 492
PaeR7I CTCGAG 1 cut(s) 600
PagI TCATGA 1 cut(s) 1129
PcsI WCGNNNNNNNCGW 2 cut(s) 240, 489
PdiI GCCGGC 2 cut(s) 487, 811
PdmI GAANNNNTTC 1 cut(s) 498
PfeI GAWTC 2 cut(s) 734, 792
PkrI GCNGC 6 cut(s) 520, 669, 896, 1023, 1090, 1110
PleI GAGTC 8 cut(s) 160, 233, 286, 384, 449, 455, 988, 1204
PpsI GAGTC 8 cut(s) 160, 233, 286, 384, 449, 455, 988, 1204
PshAI GACNNNNGTC 1 cut(s) 786
Psp6I CCWGG 2 cut(s) 143, 813
PspGI CCWGG 2 cut(s) 143, 813
PspN4I GGNNCC 4 cut(s) 331, 678, 1059, 1140
PspPI GGNCC 3 cut(s) 771, 807, 1057
PspXI VCTCGAGB 1 cut(s) 600
PsuI RGATCY 2 cut(s) 329, 568
RigI GGCCGGCC 1 cut(s) 813
SaqAI TTAA 2 cut(s) 935, 1080
SatI GCNGC 6 cut(s) 519, 668, 895, 1022, 1089, 1109
Sau3AI GATC 5 cut(s) 329, 561, 568, 684, 928
Sau96I GGNCC 3 cut(s) 771, 807, 1057
SchI GAGTC 8 cut(s) 161, 233, 287, 384, 450, 455, 989, 1205
ScrFI CCNGG 2 cut(s) 145, 815
SduI GDGCHC 3 cut(s) 681, 864, 1192
SfaNI GCATC 4 cut(s) 126, 186, 750, 1133
Sfr274I CTCGAG 1 cut(s) 600
SfuI TTCGAA 1 cut(s) 492
SinI GGWCC 1 cut(s) 771
SlaI CTCGAG 1 cut(s) 600
SmlI CTYRAG 2 cut(s) 275, 600
SmoI CTYRAG 2 cut(s) 275, 600
Sse9I AATT 5 cut(s) 651, 996, 1066, 1170, 1222
SsiI CCGC 9 cut(s) 169, 247, 300, 519, 630, 668, 894, 1088, 1108
SspMI CTAG 2 cut(s) 797, 1178
StyD4I CCNGG 2 cut(s) 143, 813
StyI CCWWGG 3 cut(s) 528, 1007, 1203
TaiI ACGT 1 cut(s) 709
TaqI TCGA 6 cut(s) 492, 535, 560, 601, 732, 790
TasI AATT 5 cut(s) 651, 996, 1066, 1170, 1222
TauI GCSGC 5 cut(s) 521, 670, 897, 1091, 1111
TfiI GAWTC 2 cut(s) 734, 792
Tru1I TTAA 2 cut(s) 935, 1080
Tru9I TTAA 2 cut(s) 935, 1080
TscAI CASTG 3 cut(s) 353, 847, 1107
TseFI GTSAC 2 cut(s) 842, 913
TseI GCWGC 1 cut(s) 1021
Tsp45I GTSAC 2 cut(s) 842, 913
TspRI CASTG 3 cut(s) 353, 847, 1107
VpaK11BI GGWCC 1 cut(s) 771
XapI RAATTY 1 cut(s) 1066
XcmI CCANNNNNNNNNTGG 2 cut(s) 141, 650
XhoI CTCGAG 1 cut(s) 600
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 2 cut(s) 797, 1178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.