pycom05g03500

Hyccin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0014272
Physical Location & Seq
Reverse (-)
4029370 .. 4030653
1284 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g03500.1

Sequence Viewer

Length: 1284 bp
ATGGACTACCACCACTTAAACCACAGCACCAACTCCCCCGCATCTTCTGCCTCCTCCACCACCACCGCCACCGCACCCAACCCTAACCCTATTTCCACCTCCAACTCCGTCTCCGCCGACGACCCCATGCACTCCTGGTGGGAGTCCATATCCAAAGCCCGCTCCCGCATCCACTCCTTATCCTCTCTTCTCGATCCTTCCCTCTCACAATCCCTCTCTTCCCTCGCCGACTCCGACCGCCCTGCCCTCTCCCTTCTCTCCTCCATTCAATCCTACGACGCCGTATCTACTGCCCTCTCCTCCCCTCTCTCAGGCTCAGGATCCGACCCCCTCTGTCACTGGCTTTACGACACCTTCCTCTCCTCCGACCCCCATCTACGCCTCGTCGTTTTCTCCTTCCTCCCCCTCCTCTCCGGTCTCTACCTCTCCCGAGTCTACTCTCCCTCCTCTGACTCCCCCTCTCTTCCCTCCCTCGCCGGCTTTGAATCCGTTCTCCTCGCCCTTTACGCCGCCGAGACCAAGGCTCGAAACGGCAAGCCTGTCGTCGTTTCGATCCCCGATCTCTCTCAGCCCTCTCTCTACCACACCCCTCGCCTCAACAAGTCCAACCCCCTCACTCTCTCCACTGCCCCCCACCAATCCATTGGGGTTTTGTCTCCGCCGCTTGAGCCCCAGGTCGCGGTGAAGTCCACGAAACGTGCCTGCATCGTCGGCGTTGCCCTCGATTCCTATTACAAGCACATTTCCCAGATGCCCACTTGGTCCAAGATCGACTTTTGTCGATTCTTAGCTTCTTGGGCCGGCCAGGATTGCTCTTGTCTGCGACAATTCGGTGATGATGATACCAACGAGCCGGATATGAATCCAGGATTGTTCTTAGAGGGCGCCAATGATGAGATCAGCGATGTGACCGAAGAAATGGATCAGTTGACGATGAGAATTGAGAGAACTGGTAGCAATGGTGTGCATTTGGAGTCAAAGGGGTCCAGAATTCCATTACCTTGGGAGCTATTGCAGCCTGCATTGCGAATATTGGGGCATTGTTTGTTGGCCCCATTGAATTATCAGGACGTTAAGGATGCCGCTTCTGTTGCAGTAAAGCGATTGTATGCTCGGGCGTCTCATGATTTGGTGCCCCAGGCAATTTTGGCTACCCAGAGTCTCATTCAGCTTGATAAGAGGGCACGCGAGGCTGCCAAGAATTCTGCGGCTGCTAATTCGTCTTCAAGTGCTAACACCCCGAGCAAAGCTAAGAAACCTGAAGTTTTTTTAGTCTCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hyccin

Protein Analysis

428

Amino Acids

46.08

Weight (kDa)

6.22

Isoelectric Point (pI)

54.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hyccin PF09790 77 - 415 5.7e-61 Hyccin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64090
fragaria_vesca FvH4_1g19990
malus_domestica MD05G1042400.v1.1 MD10G1049000.v1.1
prunus_persica Prupe.8G059200_v2.0.a1
pyrus_communis pycom05g03500
rosa_chinensis RchiOBHm_Chr2g0111931
rosa_laevigata RLG00000017939 RLG00000034552
rosa_multiflora Rmu_sc0005679.1_g000010
rosa_roxburghii Rroxscaffold_2G00131980
rosa_rugosa Rorug02G0177300
rosa_samantha Rh2AG229700 Rh2BG242400 Rh2CG233800 Rh2DG237400
rosa_wichuraiana Rw2G017770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 884, 1132
AccBSI CCGCTC 1 cut(s) 162
AccI GTMKAC 1 cut(s) 435
AccII CGCG 2 cut(s) 680, 1188
AclWI GGATC 5 cut(s) 188, 315, 328, 547, 930
AcoI YGGCCR 1 cut(s) 802
AcsI RAATTY 2 cut(s) 990, 1201
AcuI CTGAAG 1 cut(s) 1281
AcyI GRCGYC 3 cut(s) 279, 885, 1118
AfiI CCNNNNNNNGG 2 cut(s) 311, 679
AgsI TTSAA 4 cut(s) 269, 485, 1060, 1227
AjnI CCWGG 5 cut(s) 134, 672, 804, 865, 1137
AluBI AGCT 4 cut(s) 791, 1009, 1171, 1250
AluI AGCT 4 cut(s) 791, 1009, 1171, 1250
Alw26I GTCTC 7 cut(s) 115, 422, 509, 660, 1125, 1166, 1279
AlwI GGATC 5 cut(s) 188, 315, 328, 547, 930
Ama87I CYCGRG 3 cut(s) 429, 1113, 1240
AoxI GGCC 3 cut(s) 798, 802, 1050
ApeKI GCWGC 3 cut(s) 1015, 1193, 1211
ApoI RAATTY 2 cut(s) 990, 1201
Asp700I GAANNNNTTC 1 cut(s) 489
AspLEI GCGC 1 cut(s) 887
AspS9I GGNCC 4 cut(s) 762, 798, 984, 1051
AsuHPI GGTGA 2 cut(s) 694, 845
AvaI CYCGRG 3 cut(s) 429, 1113, 1240
AvaII GGWCC 2 cut(s) 762, 984
BaeGI GKGCMC 2 cut(s) 1137, 1186
BamHI GGATCC 1 cut(s) 320
BanI GGYRCC 2 cut(s) 884, 1132
BanII GRGCYC 1 cut(s) 672
BbsI GAAGAC 1 cut(s) 1215
BbvI GCAGC 3 cut(s) 1027, 1180, 1198
BccI CCATC 1 cut(s) 381
BceAI ACGGC 2 cut(s) 266, 547
BcgI CGANNNNNNTGC 4 cut(s) 224, 258, 523, 557
BciT130I CCWGG 5 cut(s) 136, 674, 806, 867, 1139
BcoDI GTCTC 7 cut(s) 115, 422, 509, 660, 1125, 1166, 1279
BfoI RGCGCY 1 cut(s) 888
BisI GCNGC 7 cut(s) 510, 662, 1016, 1083, 1194, 1209, 1212
BlsI GCNGC 7 cut(s) 511, 663, 1017, 1084, 1195, 1210, 1213
Bme1390I CCNGG 5 cut(s) 136, 674, 806, 867, 1139
Bme18I GGWCC 2 cut(s) 762, 984
BmeT110I CYCGRG 3 cut(s) 429, 1113, 1240
BmgT120I GGNCC 4 cut(s) 762, 798, 984, 1051
BmiI GGNNCC 5 cut(s) 322, 886, 985, 1053, 1134
BmrFI CCNGG 5 cut(s) 136, 674, 806, 867, 1139
BmsI GCATC 5 cut(s) 50, 177, 714, 741, 1069
BoxI GACNNNNGTC 1 cut(s) 777
BpiI GAAGAC 1 cut(s) 1215
Bpu10I CCTNAGC 1 cut(s) 316
BpuEI CTTGAG 1 cut(s) 686
BsaBI GATNNNNATC 1 cut(s) 861
BsaHI GRCGYC 3 cut(s) 279, 885, 1118
BsaI GGTCTC 2 cut(s) 422, 509
BsaJI CCNNGG 4 cut(s) 519, 672, 1001, 1137
BsaWI WCCGGW 1 cut(s) 413
BsaXI ACNNNNNCTCC 4 cut(s) 95, 125, 428, 458
Bsc4I CCNNNNNNNGG 2 cut(s) 311, 679
Bse118I RCCGGY 2 cut(s) 476, 800
Bse1I ACTGG 2 cut(s) 344, 955
Bse3DI GCAATG 2 cut(s) 964, 1022
Bse8I GATNNNNATC 1 cut(s) 861
BseBI CCWGG 5 cut(s) 136, 674, 806, 867, 1139
BseDI CCNNGG 4 cut(s) 519, 672, 1001, 1137
BseGI GGATG 2 cut(s) 168, 1084
BseJI GATNNNNATC 1 cut(s) 861
BseLI CCNNNNNNNGG 2 cut(s) 311, 679
BseMI GCAATG 2 cut(s) 964, 1022
BseMII CTCAG 3 cut(s) 324, 330, 581
BseNI ACTGG 2 cut(s) 344, 955
BseRI GAGGAG 7 cut(s) 43, 250, 289, 352, 398, 436, 485
BseSI GKGCMC 2 cut(s) 1137, 1186
BseXI GCAGC 3 cut(s) 1027, 1180, 1198
Bsh1236I CGCG 2 cut(s) 680, 1188
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 3 cut(s) 800, 804, 1052
BshNI GGYRCC 2 cut(s) 884, 1132
BsiEI CGRYCG 1 cut(s) 238
BsiHKCI CYCGRG 3 cut(s) 429, 1113, 1240
BsiSI CCGG 4 cut(s) 414, 477, 801, 854
BslI CCNNNNNNNGG 2 cut(s) 311, 679
BsmAI GTCTC 7 cut(s) 115, 422, 509, 660, 1125, 1166, 1279
BsmBI CGTCTC 2 cut(s) 115, 1125
BsnI GGCC 3 cut(s) 800, 804, 1052
Bso31I GGTCTC 2 cut(s) 422, 509
BsoBI CYCGRG 3 cut(s) 429, 1113, 1240
Bsp1286I GDGCHC 3 cut(s) 672, 1137, 1186
Bsp143I GATC 7 cut(s) 193, 320, 552, 559, 768, 897, 922
BspANI GGCC 3 cut(s) 800, 804, 1052
BspCNI CTCAG 3 cut(s) 323, 329, 580
BspFNI CGCG 2 cut(s) 680, 1188
BspHI TCATGA 1 cut(s) 1123
BspLI GGNNCC 5 cut(s) 322, 886, 985, 1053, 1134
BspPI GGATC 5 cut(s) 188, 315, 328, 547, 930
BspT107I GGYRCC 2 cut(s) 884, 1132
BspTNI GGTCTC 2 cut(s) 422, 509
BsrBI CCGCTC 1 cut(s) 162
BsrDI GCAATG 2 cut(s) 964, 1022
BsrFI RCCGGY 2 cut(s) 476, 800
BsrI ACTGG 2 cut(s) 344, 955
BssAI RCCGGY 2 cut(s) 476, 800
BssECI CCNNGG 4 cut(s) 519, 672, 1001, 1137
BssMI GATC 7 cut(s) 193, 320, 552, 559, 768, 897, 922
BssNI GRCGYC 3 cut(s) 279, 885, 1118
BssT1I CCWWGG 2 cut(s) 519, 1001
Bst2UI CCWGG 5 cut(s) 136, 674, 806, 867, 1139
Bst6I CTCTTC 3 cut(s) 192, 223, 468
BstACI GRCGYC 3 cut(s) 279, 885, 1118
BstAPI GCANNNNNTGC 1 cut(s) 47
BstC8I GCNNGC 7 cut(s) 160, 478, 536, 703, 802, 1020, 1186
BstDEI CTNAG 6 cut(s) 310, 316, 567, 787, 877, 1251
BstENI CCTNNNNNAGG 1 cut(s) 309
BstF5I GGATG 2 cut(s) 168, 1084
BstFNI CGCG 2 cut(s) 680, 1188
BstH2I RGCGCY 1 cut(s) 888
BstHHI GCGC 1 cut(s) 887
BstKTI GATC 7 cut(s) 196, 323, 555, 562, 771, 900, 925
BstMAI GTCTC 7 cut(s) 115, 422, 509, 660, 1125, 1166, 1279
BstMBI GATC 7 cut(s) 193, 320, 552, 559, 768, 897, 922
BstMCI CGRYCG 1 cut(s) 238
BstNI CCWGG 5 cut(s) 136, 674, 806, 867, 1139
BstPAI GACNNNNGTC 1 cut(s) 777
BstSCI CCNGG 5 cut(s) 134, 672, 804, 865, 1137
BstSLI GKGCMC 2 cut(s) 1137, 1186
BstUI CGCG 2 cut(s) 680, 1188
BstV1I GCAGC 3 cut(s) 1027, 1180, 1198
BstV2I GAAGAC 1 cut(s) 1215
BstX2I RGATCY 1 cut(s) 320
BstXI CCANNNNNNTGG 2 cut(s) 644, 1002
BstYI RGATCY 1 cut(s) 320
BsuRI GGCC 3 cut(s) 800, 804, 1052
BtgZI GCGATG 1 cut(s) 918
BtsCI GGATG 2 cut(s) 168, 1084
BtsI GCAGTG 1 cut(s) 624
BtsIMutI CAGTG 2 cut(s) 337, 624
Cac8I GCNNGC 7 cut(s) 160, 478, 536, 703, 802, 1020, 1186
CciI TCATGA 1 cut(s) 1123
CfoI GCGC 1 cut(s) 887
Cfr10I RCCGGY 2 cut(s) 476, 800
Cfr13I GGNCC 4 cut(s) 762, 798, 984, 1051
CseI GACGC 2 cut(s) 287, 1107
CviAII CATG 2 cut(s) 127, 1124
DdeI CTNAG 6 cut(s) 310, 316, 567, 787, 877, 1251
DinI GGCGCC 1 cut(s) 886
DpnI GATC 7 cut(s) 195, 322, 554, 561, 770, 899, 924
DpnII GATC 7 cut(s) 193, 320, 552, 559, 768, 897, 922
EaeI YGGCCR 1 cut(s) 802
Eam1104I CTCTTC 3 cut(s) 192, 223, 468
EarI CTCTTC 3 cut(s) 192, 223, 468
EciI GGCGGA 2 cut(s) 103, 648
Eco130I CCWWGG 2 cut(s) 519, 1001
Eco24I GRGCYC 1 cut(s) 672
Eco31I GGTCTC 2 cut(s) 422, 509
Eco47I GGWCC 2 cut(s) 762, 984
Eco57I CTGAAG 1 cut(s) 1281
Eco88I CYCGRG 3 cut(s) 429, 1113, 1240
EcoNI CCTNNNNNAGG 1 cut(s) 309
EcoRI GAATTC 2 cut(s) 990, 1201
EcoRII CCWGG 5 cut(s) 134, 672, 804, 865, 1137
EcoT14I CCWWGG 2 cut(s) 519, 1001
EcoT38I GRGCYC 1 cut(s) 672
EgeI GGCGCC 1 cut(s) 886
EheI GGCGCC 1 cut(s) 886
ErhI CCWWGG 2 cut(s) 519, 1001
Esp3I CGTCTC 2 cut(s) 115, 1125
FaeI CATG 2 cut(s) 130, 1127
FaiI YATR 5 cut(s) 128, 149, 860, 1110, 1125
FalI AAGNNNNNCTT 2 cut(s) 758, 790
FatI CATG 2 cut(s) 126, 1123
FauI CCCGC 3 cut(s) 46, 167, 173
FblI GTMKAC 1 cut(s) 435
Fnu4HI GCNGC 7 cut(s) 510, 662, 1016, 1083, 1194, 1209, 1212
FokI GGATG 2 cut(s) 155, 1091
FriOI GRGCYC 1 cut(s) 672
FseI GGCCGGCC 1 cut(s) 804
Fsp4HI GCNGC 7 cut(s) 510, 662, 1016, 1083, 1194, 1209, 1212
GlaI GCGC 1 cut(s) 886
GluI GCNGC 7 cut(s) 510, 662, 1016, 1083, 1194, 1209, 1212
HaeII RGCGCY 1 cut(s) 888
HaeIII GGCC 3 cut(s) 800, 804, 1052
HapII CCGG 4 cut(s) 414, 477, 801, 854
HgaI GACGC 2 cut(s) 287, 1107
HhaI GCGC 1 cut(s) 887
Hin1I GRCGYC 3 cut(s) 279, 885, 1118
Hin1II CATG 2 cut(s) 130, 1127
Hin6I GCGC 1 cut(s) 885
HinP1I GCGC 1 cut(s) 885
HincII GTYRAC 1 cut(s) 930
HindII GTYRAC 1 cut(s) 930
HpaII CCGG 4 cut(s) 414, 477, 801, 854
HphI GGTGA 2 cut(s) 694, 845
Hpy166II GTNNAC 3 cut(s) 436, 690, 930
Hpy188I TCNGA 4 cut(s) 235, 325, 367, 451
Hpy188III TCNNGA 6 cut(s) 191, 318, 429, 987, 1067, 1124
Hpy8I GTNNAC 3 cut(s) 436, 690, 930
Hpy99I CGWCG 5 cut(s) 122, 281, 389, 548, 713
HpyAV CCTTC 4 cut(s) 207, 263, 364, 406
HpyCH4IV ACGT 2 cut(s) 697, 1071
HpyCH4V TGCA 6 cut(s) 130, 705, 967, 1015, 1022, 1094
HpyF3I CTNAG 6 cut(s) 310, 316, 567, 787, 877, 1251
HpySE526I ACGT 2 cut(s) 697, 1071
Hsp92I GRCGYC 3 cut(s) 279, 885, 1118
Hsp92II CATG 2 cut(s) 130, 1127
HspAI GCGC 1 cut(s) 885
KasI GGCGCC 1 cut(s) 884
KroI GCCGGC 2 cut(s) 476, 800
KroNI GCCGGC 2 cut(s) 478, 802
Kzo9I GATC 7 cut(s) 193, 320, 552, 559, 768, 897, 922
LmnI GCTCC 2 cut(s) 167, 1006
Lsp1109I GCAGC 3 cut(s) 1027, 1180, 1198
LweI GCATC 5 cut(s) 50, 177, 714, 741, 1069
MaeII ACGT 2 cut(s) 697, 1071
MaeIII GTNAC 2 cut(s) 335, 907
MalI GATC 7 cut(s) 195, 322, 554, 561, 770, 899, 924
MbiI CCGCTC 1 cut(s) 162
MboI GATC 7 cut(s) 193, 320, 552, 559, 768, 897, 922
MboII GAAGA 6 cut(s) 36, 179, 210, 455, 926, 1215
MflI RGATCY 1 cut(s) 320
MhlI GDGCHC 3 cut(s) 672, 1137, 1186
MluCI AATT 7 cut(s) 827, 939, 990, 1060, 1143, 1201, 1216
Mly113I GGCGCC 1 cut(s) 885
MlyI GAGTC 6 cut(s) 152, 224, 441, 446, 983, 1168
MmeI TCCRAC 5 cut(s) 126, 258, 348, 390, 630
MroNI GCCGGC 2 cut(s) 476, 800
MroXI GAANNNNTTC 1 cut(s) 489
MseI TTAA 2 cut(s) 17, 1074
MspI CCGG 4 cut(s) 414, 477, 801, 854
MspR9I CCNGG 5 cut(s) 136, 674, 806, 867, 1139
MvaI CCWGG 5 cut(s) 136, 674, 806, 867, 1139
MvnI CGCG 2 cut(s) 680, 1188
NaeI GCCGGC 2 cut(s) 478, 802
NarI GGCGCC 1 cut(s) 885
NdeII GATC 7 cut(s) 193, 320, 552, 559, 768, 897, 922
NgoMIV GCCGGC 2 cut(s) 476, 800
NlaIII CATG 2 cut(s) 130, 1127
NlaIV GGNNCC 5 cut(s) 322, 886, 985, 1053, 1134
NmeAIII GCCGAG 1 cut(s) 538
NmuCI GTSAC 2 cut(s) 335, 907
PagI TCATGA 1 cut(s) 1123
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PdiI GCCGGC 2 cut(s) 478, 802
PdmI GAANNNNTTC 1 cut(s) 489
PfeI GAWTC 4 cut(s) 485, 725, 783, 862
PfoI TCCNGGA 1 cut(s) 865
PkrI GCNGC 7 cut(s) 511, 663, 1017, 1084, 1195, 1210, 1213
PleI GAGTC 6 cut(s) 151, 224, 440, 446, 982, 1167
PluTI GGCGCC 1 cut(s) 888
PpsI GAGTC 6 cut(s) 151, 224, 440, 446, 982, 1167
PshAI GACNNNNGTC 1 cut(s) 777
Psp6I CCWGG 5 cut(s) 134, 672, 804, 865, 1137
PspGI CCWGG 5 cut(s) 134, 672, 804, 865, 1137
PspN4I GGNNCC 5 cut(s) 322, 886, 985, 1053, 1134
PspPI GGNCC 4 cut(s) 762, 798, 984, 1051
PsuI RGATCY 1 cut(s) 320
RigI GGCCGGCC 1 cut(s) 804
SaqAI TTAA 2 cut(s) 17, 1074
SatI GCNGC 7 cut(s) 510, 662, 1016, 1083, 1194, 1209, 1212
Sau3AI GATC 7 cut(s) 193, 320, 552, 559, 768, 897, 922
Sau96I GGNCC 4 cut(s) 762, 798, 984, 1051
SchI GAGTC 6 cut(s) 152, 224, 441, 446, 983, 1168
ScrFI CCNGG 5 cut(s) 136, 674, 806, 867, 1139
SduI GDGCHC 3 cut(s) 672, 1137, 1186
SfaNI GCATC 5 cut(s) 50, 177, 714, 741, 1069
SfoI GGCGCC 1 cut(s) 886
SinI GGWCC 2 cut(s) 762, 984
SmlI CTYRAG 1 cut(s) 665
SmoI CTYRAG 1 cut(s) 665
Sse9I AATT 7 cut(s) 827, 939, 990, 1060, 1143, 1201, 1216
SspDI GGCGCC 1 cut(s) 884
SspI AATATT 1 cut(s) 1032
StyD4I CCNGG 5 cut(s) 134, 672, 804, 865, 1137
StyI CCWWGG 2 cut(s) 519, 1001
TaiI ACGT 2 cut(s) 700, 1074
TaqI TCGA 6 cut(s) 192, 526, 551, 723, 771, 781
TaqII GACCGA 1 cut(s) 926
TasI AATT 7 cut(s) 827, 939, 990, 1060, 1143, 1201, 1216
TauI GCSGC 4 cut(s) 512, 664, 1085, 1211
TfiI GAWTC 4 cut(s) 485, 725, 783, 862
Tru1I TTAA 2 cut(s) 17, 1074
Tru9I TTAA 2 cut(s) 17, 1074
TscAI CASTG 2 cut(s) 344, 631
TseFI GTSAC 2 cut(s) 335, 907
TseI GCWGC 3 cut(s) 1015, 1193, 1211
Tsp45I GTSAC 2 cut(s) 335, 907
TspDTI ATGAA 1 cut(s) 875
TspGWI ACGGA 2 cut(s) 97, 478
TspRI CASTG 2 cut(s) 344, 631
VpaK11BI GGWCC 2 cut(s) 762, 984
XagI CCTNNNNNAGG 1 cut(s) 309
XapI RAATTY 2 cut(s) 990, 1201
XcmI CCANNNNNNNNNTGG 1 cut(s) 641
XmiI GTMKAC 1 cut(s) 435
XmnI GAANNNNTTC 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.