Rw2G017770

Hyccin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014272
Physical Location & Seq
Forward (+)
22117990 .. 22119324
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G017770.1

Sequence Viewer

Length: 1335 bp
ATGGACCTCTACCCAAATCACAGCACCAACTCCCCCACCTCCTCCGCCTCCTCCACCGCCACCAACCCAAACCCCAGACCCACCCGCAACACCATATCCTCCCTCACCGCCACCGACGACGACCCAATGCACTCCTGGTGGGAGTCCATCTCCAAAGCCCGCTCCCGCATCCACTCCCTCGCCACTCTCCTCGACCCTTCTCTCTCCTCCTTCCTCTCCTCCCTCGCCGACTCCGACCGCCCCGCCCTCTCTCTCCTCTCCTCCCTTGAAGCCTACGCCGCCGTTTCCGCCGTCCTCTCCGCCCCCCTCTCCGGCTCCGGACCCGACCCGCTCTGCAACTGGCTCTACGACACCTTCCTCTCCTCCGACCCCCATCTACGACTCGTCGTTTTCTCCTTCCTCCCTCTCCTCGCCGGCCTCTACCTCTCCCGCATCCACTCTCTCTCCTCTGACTCTCTTACCTCGCTTCCCTCCCTCGCCGGCTTTGAGGCCGTGCTCCTCGCTCTCTACGCCGCAGAGACCAAGGCCCGAAACGGCAAGCCCATTGTCGTTTCGATCCCTGACCTATCTCAGCCCTCTCTCTACCACATTCCTCCAAGCCAGAAGACGACTAATCCCTACTCCAATTCCAGTAATATCCAATCCGTTGGGGTTTTGTCGCCGCCGCTGGAGCCCCAGGTCGCGGTCAAGTCAACCAAACGGGCCTGCATTGTGGGAGTTGCGCTGGATTCTTATTACAAGCACATTTCGCAGATGCCCACTTGGTCCAAGATGGATTTTTGCAGGTTCCTAGCTTCTTGGGCCGGCCCGGATTGCTCTTGTCAGCAGCAATTTGAATCCGGTGATAACCACAGTGAGCCAGAGATTGCTGGGTTCTTGGAGGCCGGCAATGGGAATGGGAGTGGTAATGAGTATGAGAATCAGAGTGAGATTGAGATTGAGGGTGATGTGGCGGAAGAAATGGGTCAACTAAGAATTGAGCAAAATGGGATCAGCAGTGGGGTCAGTCATGTGGAGGACTCAAAGGGGGTGAAGATTCCATTGCCGTGGGAGCTTTTGCAGCCAGCGCTGCGAATATTGGGGCATTGTTTGTTGGCGCCATTGAATTCTCAGGAGGTTAAGGACGCTGCTTCCATTGCGGTGAGGAGATTGTATGCCAGGGCATCTCATGATTTGGTGCCGCAGGCGATTTTAGCTACTCAGAGTCTCATTCAGCTTGATAACAGGGGGCGTGAGGCAGCCAAGGATGCAGTGGCGGTTGTTGTTAATTCATCTTCAAATGTTAACACGCCGAGCAAAGCTAAGAAACCTGAAATCCTTTTGGTCTCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hyccin

Protein Analysis

444

Amino Acids

47.69

Weight (kDa)

5.48

Isoelectric Point (pI)

54.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hyccin PF09790 77 - 405 1.1e-59 Hyccin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64090
fragaria_vesca FvH4_1g19990
malus_domestica MD05G1042400.v1.1 MD10G1049000.v1.1
prunus_persica Prupe.8G059200_v2.0.a1
pyrus_communis pycom05g03500
rosa_chinensis RchiOBHm_Chr2g0111931
rosa_laevigata RLG00000017939 RLG00000034552
rosa_multiflora Rmu_sc0005679.1_g000010
rosa_roxburghii Rroxscaffold_2G00131980
rosa_rugosa Rorug02G0177300
rosa_samantha Rh2AG229700 Rh2BG242400 Rh2CG233800 Rh2DG237400
rosa_wichuraiana Rw2G017770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 774
AccB1I GGYRCC 2 cut(s) 1096, 1177
AccBSI CCGCTC 2 cut(s) 162, 331
AccII CGCG 1 cut(s) 683
AccIII TCCGGA 1 cut(s) 317
AclWI GGATC 2 cut(s) 550, 998
AcsI RAATTY 1 cut(s) 1105
AcyI GRCGYC 1 cut(s) 1097
AfeI AGCGCT 1 cut(s) 1068
AfiI CCNNNNNNNGG 3 cut(s) 311, 682, 891
AgsI TTSAA 4 cut(s) 269, 836, 1105, 1278
AjnI CCWGG 3 cut(s) 134, 675, 1157
AluBI AGCT 5 cut(s) 794, 1054, 1196, 1216, 1301
AluI AGCT 5 cut(s) 794, 1054, 1196, 1216, 1301
Alw21I GWGCWC 1 cut(s) 498
Alw26I GTCTC 3 cut(s) 512, 1211, 1330
AlwI GGATC 2 cut(s) 550, 998
Aor13HI TCCGGA 1 cut(s) 317
Aor51HI AGCGCT 1 cut(s) 1068
AoxI GGCC 7 cut(s) 415, 489, 525, 702, 801, 805, 882
ApeKI GCWGC 5 cut(s) 826, 1060, 1069, 1127, 1238
ApoI RAATTY 1 cut(s) 1105
AspLEI GCGC 3 cut(s) 724, 1069, 1099
AspS9I GGNCC 7 cut(s) 4, 320, 526, 702, 765, 801, 806
AsuC2I CCSGG 1 cut(s) 809
AsuHPI GGTGA 5 cut(s) 97, 854, 956, 1042, 1153
AvaII GGWCC 3 cut(s) 4, 320, 765
BanI GGYRCC 2 cut(s) 1096, 1177
BanII GRGCYC 1 cut(s) 675
BbsI GAAGAC 1 cut(s) 611
Bbv12I GWGCWC 1 cut(s) 498
BbvI GCAGC 5 cut(s) 838, 1056, 1072, 1114, 1250
BccI CCATC 3 cut(s) 155, 381, 766
BceAI ACGGC 5 cut(s) 266, 275, 476, 550, 1030
BciT130I CCWGG 3 cut(s) 136, 677, 1159
BcnI CCSGG 1 cut(s) 809
BcoDI GTCTC 3 cut(s) 512, 1211, 1330
BfaI CTAG 1 cut(s) 791
BfoI RGCGCY 2 cut(s) 1070, 1100
BfuAI ACCTGC 1 cut(s) 774
Bme1390I CCNGG 4 cut(s) 136, 677, 809, 1159
Bme18I GGWCC 3 cut(s) 4, 320, 765
BmgT120I GGNCC 7 cut(s) 4, 320, 526, 702, 765, 801, 806
BmiI GGNNCC 6 cut(s) 316, 322, 672, 788, 1098, 1179
BmrFI CCNGG 4 cut(s) 136, 677, 809, 1159
BmsI GCATC 5 cut(s) 177, 441, 744, 1172, 1237
BpiI GAAGAC 1 cut(s) 611
BplI GAGNNNNNCTC 2 cut(s) 134, 166
BpmI CTGGAG 1 cut(s) 689
BpuMI CCSGG 1 cut(s) 809
BsaHI GRCGYC 1 cut(s) 1097
BsaI GGTCTC 2 cut(s) 512, 1330
BsaJI CCNNGG 5 cut(s) 522, 675, 1046, 1158, 1242
BsaWI WCCGGW 2 cut(s) 317, 839
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bsc4I CCNNNNNNNGG 3 cut(s) 311, 682, 891
Bse118I RCCGGY 4 cut(s) 413, 479, 803, 884
Bse1I ACTGG 2 cut(s) 344, 630
Bse3DI GCAATG 3 cut(s) 895, 1040, 1134
BseAI TCCGGA 1 cut(s) 317
BseBI CCWGG 3 cut(s) 136, 677, 1159
BseDI CCNNGG 5 cut(s) 522, 675, 1046, 1158, 1242
BseGI GGATG 3 cut(s) 168, 432, 1252
BseLI CCNNNNNNNGG 3 cut(s) 311, 682, 891
BseMI GCAATG 3 cut(s) 895, 1040, 1134
BseMII CTCAG 3 cut(s) 584, 1124, 1214
BseNI ACTGG 2 cut(s) 344, 630
BseXI GCAGC 5 cut(s) 838, 1056, 1072, 1114, 1250
BseYI CCCAGC 1 cut(s) 869
Bsh1236I CGCG 1 cut(s) 683
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 7 cut(s) 417, 491, 527, 704, 803, 807, 884
BshNI GGYRCC 2 cut(s) 1096, 1177
BsiEI CGRYCG 1 cut(s) 238
BsiHKAI GWGCWC 1 cut(s) 498
BsiSI CCGG 8 cut(s) 312, 318, 414, 480, 804, 809, 840, 885
BslI CCNNNNNNNGG 3 cut(s) 311, 682, 891
BsmAI GTCTC 3 cut(s) 512, 1211, 1330
BsnI GGCC 7 cut(s) 417, 491, 527, 704, 803, 807, 884
Bso31I GGTCTC 2 cut(s) 512, 1330
Bsp1286I GDGCHC 2 cut(s) 498, 675
Bsp13I TCCGGA 1 cut(s) 317
Bsp143I GATC 2 cut(s) 555, 990
BspANI GGCC 7 cut(s) 417, 491, 527, 704, 803, 807, 884
BspCNI CTCAG 3 cut(s) 583, 1123, 1213
BspEI TCCGGA 1 cut(s) 317
BspFNI CGCG 1 cut(s) 683
BspHI TCATGA 1 cut(s) 1168
BspLI GGNNCC 6 cut(s) 316, 322, 672, 788, 1098, 1179
BspMI ACCTGC 1 cut(s) 774
BspPI GGATC 2 cut(s) 550, 998
BspT107I GGYRCC 2 cut(s) 1096, 1177
BspTNI GGTCTC 2 cut(s) 512, 1330
BsrBI CCGCTC 2 cut(s) 162, 331
BsrDI GCAATG 3 cut(s) 895, 1040, 1134
BsrFI RCCGGY 4 cut(s) 413, 479, 803, 884
BsrI ACTGG 2 cut(s) 344, 630
BssAI RCCGGY 4 cut(s) 413, 479, 803, 884
BssECI CCNNGG 5 cut(s) 522, 675, 1046, 1158, 1242
BssMI GATC 2 cut(s) 555, 990
BssNI GRCGYC 1 cut(s) 1097
BssT1I CCWWGG 2 cut(s) 522, 1242
Bst2UI CCWGG 3 cut(s) 136, 677, 1159
Bst4CI ACNGT 1 cut(s) 854
BstACI GRCGYC 1 cut(s) 1097
BstC8I GCNNGC 9 cut(s) 160, 415, 481, 539, 706, 805, 886, 1065, 1185
BstDEI CTNAG 5 cut(s) 570, 971, 1110, 1200, 1302
BstDSI CCRYGG 1 cut(s) 1046
BstF5I GGATG 3 cut(s) 168, 432, 1252
BstFNI CGCG 1 cut(s) 683
BstH2I RGCGCY 2 cut(s) 1070, 1100
BstHHI GCGC 3 cut(s) 724, 1069, 1099
BstKTI GATC 2 cut(s) 558, 993
BstMAI GTCTC 3 cut(s) 512, 1211, 1330
BstMBI GATC 2 cut(s) 555, 990
BstMCI CGRYCG 1 cut(s) 238
BstNI CCWGG 3 cut(s) 136, 677, 1159
BstSCI CCNGG 4 cut(s) 134, 675, 807, 1157
BstUI CGCG 1 cut(s) 683
BstV1I GCAGC 5 cut(s) 838, 1056, 1072, 1114, 1250
BstV2I GAAGAC 1 cut(s) 611
BstXI CCANNNNNNTGG 2 cut(s) 647, 1047
BsuRI GGCC 7 cut(s) 417, 491, 527, 704, 803, 807, 884
BtgI CCRYGG 1 cut(s) 1046
BtsCI GGATG 3 cut(s) 168, 432, 1252
BtsI GCAGTG 2 cut(s) 1003, 1257
BtsIMutI CAGTG 3 cut(s) 859, 1003, 1257
BveI ACCTGC 1 cut(s) 774
Cac8I GCNNGC 9 cut(s) 160, 415, 481, 539, 706, 805, 886, 1065, 1185
CciI TCATGA 1 cut(s) 1168
CfoI GCGC 3 cut(s) 724, 1069, 1099
Cfr10I RCCGGY 4 cut(s) 413, 479, 803, 884
Cfr13I GGNCC 7 cut(s) 4, 320, 526, 702, 765, 801, 806
CseI GACGC 1 cut(s) 1133
CviAII CATG 2 cut(s) 1010, 1169
DdeI CTNAG 5 cut(s) 570, 971, 1110, 1200, 1302
DinI GGCGCC 1 cut(s) 1098
DpnI GATC 2 cut(s) 557, 992
DpnII GATC 2 cut(s) 555, 990
EciI GGCGGA 4 cut(s) 34, 277, 289, 968
Eco130I CCWWGG 2 cut(s) 522, 1242
Eco24I GRGCYC 1 cut(s) 675
Eco31I GGTCTC 2 cut(s) 512, 1330
Eco47I GGWCC 3 cut(s) 4, 320, 765
Eco47III AGCGCT 1 cut(s) 1068
EcoRI GAATTC 1 cut(s) 1105
EcoRII CCWGG 3 cut(s) 134, 675, 1157
EcoT14I CCWWGG 2 cut(s) 522, 1242
EcoT38I GRGCYC 1 cut(s) 675
EgeI GGCGCC 1 cut(s) 1098
EheI GGCGCC 1 cut(s) 1098
ErhI CCWWGG 2 cut(s) 522, 1242
FaeI CATG 2 cut(s) 1013, 1172
FaiI YATR 5 cut(s) 95, 915, 1011, 1155, 1170
FatI CATG 2 cut(s) 1009, 1168
FauI CCCGC 6 cut(s) 92, 167, 173, 250, 336, 437
FokI GGATG 3 cut(s) 155, 419, 1259
FriOI GRGCYC 1 cut(s) 675
FseI GGCCGGCC 1 cut(s) 807
FspBI CTAG 1 cut(s) 791
GlaI GCGC 3 cut(s) 723, 1068, 1098
GsaI CCCAGC 1 cut(s) 873
GsuI CTGGAG 1 cut(s) 689
HaeII RGCGCY 2 cut(s) 1070, 1100
HaeIII GGCC 7 cut(s) 417, 491, 527, 704, 803, 807, 884
HapII CCGG 8 cut(s) 312, 318, 414, 480, 804, 809, 840, 885
HgaI GACGC 1 cut(s) 1133
HhaI GCGC 3 cut(s) 724, 1069, 1099
Hin1I GRCGYC 1 cut(s) 1097
Hin1II CATG 2 cut(s) 1013, 1172
Hin6I GCGC 3 cut(s) 722, 1067, 1097
HinP1I GCGC 3 cut(s) 722, 1067, 1097
HincII GTYRAC 3 cut(s) 693, 968, 1285
HindII GTYRAC 3 cut(s) 693, 968, 1285
HpaI GTTAAC 1 cut(s) 1285
HpaII CCGG 8 cut(s) 312, 318, 414, 480, 804, 809, 840, 885
HphI GGTGA 5 cut(s) 97, 854, 956, 1042, 1153
Hpy166II GTNNAC 3 cut(s) 693, 968, 1285
Hpy188I TCNGA 5 cut(s) 235, 367, 451, 924, 1203
Hpy188III TCNNGA 3 cut(s) 318, 1112, 1169
Hpy8I GTNNAC 3 cut(s) 693, 968, 1285
Hpy99I CGWCG 3 cut(s) 119, 122, 389
HpyAV CCTTC 4 cut(s) 207, 220, 364, 406
HpyCH4III ACNGT 1 cut(s) 854
HpyCH4V TGCA 6 cut(s) 130, 336, 708, 783, 1060, 1250
HpyF3I CTNAG 5 cut(s) 570, 971, 1110, 1200, 1302
Hsp92I GRCGYC 1 cut(s) 1097
Hsp92II CATG 2 cut(s) 1013, 1172
HspAI GCGC 3 cut(s) 722, 1067, 1097
KasI GGCGCC 1 cut(s) 1096
Kpn2I TCCGGA 1 cut(s) 317
KroI GCCGGC 4 cut(s) 413, 479, 803, 884
KroNI GCCGGC 4 cut(s) 415, 481, 805, 886
KspAI GTTAAC 1 cut(s) 1285
Kzo9I GATC 2 cut(s) 555, 990
LmnI GCTCC 5 cut(s) 167, 320, 501, 670, 1051
Lsp1109I GCAGC 5 cut(s) 838, 1056, 1072, 1114, 1250
LweI GCATC 5 cut(s) 177, 441, 744, 1172, 1237
MaeI CTAG 1 cut(s) 791
MalI GATC 2 cut(s) 557, 992
MbiI CCGCTC 2 cut(s) 162, 331
MboI GATC 2 cut(s) 555, 990
MboII GAAGA 4 cut(s) 616, 968, 1045, 1266
MhlI GDGCHC 2 cut(s) 498, 675
MluCI AATT 5 cut(s) 625, 830, 975, 1105, 1267
Mly113I GGCGCC 1 cut(s) 1097
MlyI GAGTC 6 cut(s) 152, 224, 375, 446, 1013, 1213
MmeI TCCRAC 2 cut(s) 258, 390
MroI TCCGGA 1 cut(s) 317
MroNI GCCGGC 4 cut(s) 413, 479, 803, 884
MseI TTAA 3 cut(s) 1119, 1266, 1284
MslI CAYNNNNRTG 2 cut(s) 1045, 1139
MspA1I CMGCKG 1 cut(s) 667
MspI CCGG 8 cut(s) 312, 318, 414, 480, 804, 809, 840, 885
MspR9I CCNGG 4 cut(s) 136, 677, 809, 1159
MvaI CCWGG 3 cut(s) 136, 677, 1159
MvnI CGCG 1 cut(s) 683
NaeI GCCGGC 4 cut(s) 415, 481, 805, 886
NarI GGCGCC 1 cut(s) 1097
NciI CCSGG 1 cut(s) 809
NdeII GATC 2 cut(s) 555, 990
NgoMIV GCCGGC 4 cut(s) 413, 479, 803, 884
NlaIII CATG 2 cut(s) 1013, 1172
NlaIV GGNNCC 6 cut(s) 316, 322, 672, 788, 1098, 1179
NmeAIII GCCGAG 1 cut(s) 1317
PagI TCATGA 1 cut(s) 1168
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PdiI GCCGGC 4 cut(s) 415, 481, 805, 886
PfeI GAWTC 4 cut(s) 728, 836, 919, 1036
PleI GAGTC 6 cut(s) 151, 224, 375, 446, 1013, 1212
PluTI GGCGCC 1 cut(s) 1100
PpsI GAGTC 6 cut(s) 151, 224, 375, 446, 1013, 1212
Psp6I CCWGG 3 cut(s) 134, 675, 1157
PspFI CCCAGC 1 cut(s) 869
PspGI CCWGG 3 cut(s) 134, 675, 1157
PspN4I GGNNCC 6 cut(s) 316, 322, 672, 788, 1098, 1179
PspPI GGNCC 7 cut(s) 4, 320, 526, 702, 765, 801, 806
RigI GGCCGGCC 1 cut(s) 807
RseI CAYNNNNRTG 2 cut(s) 1045, 1139
SaqAI TTAA 3 cut(s) 1119, 1266, 1284
Sau3AI GATC 2 cut(s) 555, 990
Sau96I GGNCC 7 cut(s) 4, 320, 526, 702, 765, 801, 806
SchI GAGTC 6 cut(s) 152, 224, 375, 446, 1013, 1213
ScrFI CCNGG 4 cut(s) 136, 677, 809, 1159
SduI GDGCHC 2 cut(s) 498, 675
SfaNI GCATC 5 cut(s) 177, 441, 744, 1172, 1237
SfoI GGCGCC 1 cut(s) 1098
SinI GGWCC 3 cut(s) 4, 320, 765
SmiMI CAYNNNNRTG 2 cut(s) 1045, 1139
Sse9I AATT 5 cut(s) 625, 830, 975, 1105, 1267
SspDI GGCGCC 1 cut(s) 1096
SspI AATATT 1 cut(s) 1077
SspMI CTAG 1 cut(s) 791
StyD4I CCNGG 4 cut(s) 134, 675, 807, 1157
StyI CCWWGG 2 cut(s) 522, 1242
TaaI ACNGT 1 cut(s) 854
TaqI TCGA 2 cut(s) 192, 554
TasI AATT 5 cut(s) 625, 830, 975, 1105, 1267
TauI GCSGC 5 cut(s) 281, 515, 664, 667, 1183
TfiI GAWTC 4 cut(s) 728, 836, 919, 1036
Tru1I TTAA 3 cut(s) 1119, 1266, 1284
Tru9I TTAA 3 cut(s) 1119, 1266, 1284
TscAI CASTG 3 cut(s) 859, 1003, 1257
TseI GCWGC 5 cut(s) 826, 1060, 1069, 1127, 1238
TspDTI ATGAA 1 cut(s) 1260
TspGWI ACGGA 1 cut(s) 634
TspRI CASTG 3 cut(s) 859, 1003, 1257
VpaK11BI GGWCC 3 cut(s) 4, 320, 765
XapI RAATTY 1 cut(s) 1105
XcmI CCANNNNNNNNNTGG 2 cut(s) 132, 1249
XspI CTAG 1 cut(s) 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.