FvH4_1g19990

Hyccin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014272
Physical Location & Seq
Forward (+)
12110514 .. 12113033
2520 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g19990.t1

Sequence Viewer

Length: 1350 bp
ATGGACCTCCACCCAAATCACAGCTCCAACTCCCCCACCTCCTCCGCCTCCTCCACCGCCACCGCCACCGCCACCGCCACCAACCCAAACCCTAACCCAAACCCCAGACCCACCACCCGCAACACCCTCTCCTCGCTCACCGCTACCGACGACGACCCAATGCACTCCTGGTGGGAATCCATCTCCAAAGCCCGCTCCCGCATCCACTCCCTCGCCACTCTCCTCCCTCCTTCTCTCTCCTCCCCTCTCTCCTCCCTCGCCGACTCCGACCGCCCCGCCCTCTCTCTCCTCTCCTCCCTCCACGCCTACTCCGCCGTGTCCGCCGTCCTCTCCTCCCCTCTCTCCGGCTCCGGATCCGACCCCCTCTGCAACTGGCTCTACGACACTTTCCTCTCCTCCGAACCCCATCTACGACTCGTCGTTTTCTCCTTCCTCCCTCTCCTCTCCGGCCTCTACCTCTCCAGAATCCACTCTCTCTCCTCTGACTCTTTGACCTCTCTTCCCTCCCTCGCCGGCTTCGAGGCGGTTCTCCTCGCTCTCTACGCCGCCGAGACCAAGGCCCGGAACGGCAAGCCTGTTGTCGTTTCGATCCCGGACCTCTCTCAGCCTTCACTCTACCACATTCCGGCTCGGAGCCCCAAGATGAGTATTTCGAACTCGAATGGCAGTAATGTGCAATCTATCGGGGTTCTGTCGCCGCCGCTGGAGCCTCAGGTGGCCGTGAAGTCGACCAAACGGGCCTGCATTGTCGGAGTTGCGCTGGACTCTTACTACAAGCACATTTCACAGATGCCGGCTTGGTCCAAGATAGAGTTTTGCAGGTTCCTAACTTCTTGGGCCGGCCAAGATTGCTCTTGTCAGAAGCAATTCGAATCCGGTGATAGCCATGGTGAGCCGGAGATTGCTGGGTTCTCGGAGGCTGGGAGTGGTAATGAGAATGAGAGTGAGATTGGGATTGAGGATGATGTGGCGGAAGAAATGGGTCAACTGAGAATTGAGCAAAATGGGAACGGTAATGGTGTCAGTCATGTGGAGGAGTCGAAGGGGGCAAGGATTCCATTGCCATGGGAGCTTTTGCAGCCGGCGCTACGCATATTGGGGCATTGTTTGTTGGGGCCTTTGAATTCTCAGGATGTTAAGGATGCTGCTTCTGTTGCGGTGAGGAGATTGTATGCCAGGGCATCTCATGATTTGGTGCCGCAGGCAATTTTAGCTACTCAGAGTCTCATTCAGCTTGATAACAGGGGGCGTGAGGCGGCCAAGGCTGTGGCGGCTGGTGCTAATTCTGCTTCAAATGTTAACACGCCGAGCAAAGCTAAGAAACCTGAAATCCTTTTGGTCTCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hyccin

Protein Analysis

450

Amino Acids

47.73

Weight (kDa)

6.12

Isoelectric Point (pI)

57.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hyccin PF09790 88 - 411 1.3e-61 Hyccin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64090
fragaria_vesca FvH4_1g19990
malus_domestica MD05G1042400.v1.1 MD10G1049000.v1.1
prunus_persica Prupe.8G059200_v2.0.a1
pyrus_communis pycom05g03500
rosa_chinensis RchiOBHm_Chr2g0111931
rosa_laevigata RLG00000017939 RLG00000034552
rosa_multiflora Rmu_sc0005679.1_g000010
rosa_roxburghii Rroxscaffold_2G00131980
rosa_rugosa Rorug02G0177300
rosa_samantha Rh2AG229700 Rh2BG242400 Rh2CG233800 Rh2DG237400
rosa_wichuraiana Rw2G017770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 810
AccB1I GGYRCC 1 cut(s) 1195
AccBSI CCGCTC 1 cut(s) 195
AccI GTMKAC 1 cut(s) 728
AccIII TCCGGA 1 cut(s) 350
AclWI GGATC 3 cut(s) 348, 361, 583
AcoI YGGCCR 3 cut(s) 717, 841, 1257
AcsI RAATTY 1 cut(s) 1123
AfiI CCNNNNNNNGG 3 cut(s) 344, 561, 625
AgsI TTSAA 2 cut(s) 1123, 1293
AjnI CCWGG 2 cut(s) 167, 1175
AluBI AGCT 5 cut(s) 24, 1072, 1214, 1234, 1316
AluI AGCT 5 cut(s) 24, 1072, 1214, 1234, 1316
Alw26I GTCTC 3 cut(s) 545, 1229, 1345
AlwI GGATC 3 cut(s) 348, 361, 583
Aor13HI TCCGGA 1 cut(s) 350
AoxI GGCC 8 cut(s) 448, 558, 717, 738, 837, 841, 1115, 1257
ApeKI GCWGC 2 cut(s) 1078, 1145
ApoI RAATTY 1 cut(s) 1123
Asp700I GAANNNNTTC 1 cut(s) 866
AspLEI GCGC 2 cut(s) 760, 1087
AspS9I GGNCC 7 cut(s) 4, 559, 595, 738, 801, 837, 1115
AsuC2I CCSGG 2 cut(s) 562, 593
AsuHPI GGTGA 4 cut(s) 130, 890, 902, 1171
AsuII TTCGAA 2 cut(s) 653, 870
AvaII GGWCC 3 cut(s) 4, 595, 801
AxyI CCTNAGG 1 cut(s) 711
BamHI GGATCC 1 cut(s) 353
BanI GGYRCC 1 cut(s) 1195
BanII GRGCYC 1 cut(s) 638
BbvI GCAGC 2 cut(s) 1090, 1132
BccI CCATC 2 cut(s) 188, 414
BceAI ACGGC 4 cut(s) 299, 308, 583, 704
BciT130I CCWGG 2 cut(s) 169, 1177
BcnI CCSGG 2 cut(s) 562, 593
BcoDI GTCTC 3 cut(s) 545, 1229, 1345
BfoI RGCGCY 1 cut(s) 1088
BfuAI ACCTGC 1 cut(s) 810
BisI GCNGC 8 cut(s) 546, 698, 701, 1079, 1146, 1199, 1257, 1272
BlsI GCNGC 8 cut(s) 547, 699, 702, 1080, 1147, 1200, 1258, 1273
Bme1390I CCNGG 4 cut(s) 169, 562, 593, 1177
Bme18I GGWCC 3 cut(s) 4, 595, 801
BmgT120I GGNCC 7 cut(s) 4, 559, 595, 738, 801, 837, 1115
BmiI GGNNCC 7 cut(s) 349, 355, 635, 708, 824, 1116, 1197
BmrFI CCNGG 4 cut(s) 169, 562, 593, 1177
BmsI GCATC 4 cut(s) 210, 780, 1132, 1190
BpmI CTGGAG 2 cut(s) 445, 725
Bpu14I TTCGAA 2 cut(s) 653, 870
BpuMI CCSGG 2 cut(s) 562, 593
BsaI GGTCTC 2 cut(s) 545, 1345
BsaJI CCNNGG 5 cut(s) 555, 886, 1064, 1176, 1260
BsaWI WCCGGW 2 cut(s) 350, 875
BsaXI ACNNNNNCTCC 6 cut(s) 113, 143, 293, 323, 461, 491
Bsc4I CCNNNNNNNGG 3 cut(s) 344, 561, 625
Bse118I RCCGGY 4 cut(s) 512, 793, 839, 1081
Bse1I ACTGG 1 cut(s) 377
Bse21I CCTNAGG 1 cut(s) 711
Bse3DI GCAATG 1 cut(s) 1058
BseAI TCCGGA 1 cut(s) 350
BseBI CCWGG 2 cut(s) 169, 1177
BseDI CCNNGG 5 cut(s) 555, 886, 1064, 1176, 1260
BseGI GGATG 4 cut(s) 201, 967, 1138, 1147
BseLI CCNNNNNNNGG 3 cut(s) 344, 561, 625
BseMI GCAATG 1 cut(s) 1058
BseMII CTCAG 5 cut(s) 617, 725, 980, 1142, 1232
BseNI ACTGG 1 cut(s) 377
BseXI GCAGC 2 cut(s) 1090, 1132
BseYI CCCAGC 2 cut(s) 905, 920
Bsh1285I CGRYCG 1 cut(s) 271
BshFI GGCC 8 cut(s) 450, 560, 719, 740, 839, 843, 1117, 1259
BshNI GGYRCC 1 cut(s) 1195
BsiEI CGRYCG 1 cut(s) 271
BslI CCNNNNNNNGG 3 cut(s) 344, 561, 625
BsmAI GTCTC 3 cut(s) 545, 1229, 1345
BsnI GGCC 8 cut(s) 450, 560, 719, 740, 839, 843, 1117, 1259
Bso31I GGTCTC 2 cut(s) 545, 1345
Bsp119I TTCGAA 2 cut(s) 653, 870
Bsp1286I GDGCHC 1 cut(s) 638
Bsp13I TCCGGA 1 cut(s) 350
Bsp143I GATC 2 cut(s) 353, 588
Bsp19I CCATGG 2 cut(s) 886, 1064
BspANI GGCC 8 cut(s) 450, 560, 719, 740, 839, 843, 1117, 1259
BspCNI CTCAG 5 cut(s) 616, 724, 981, 1141, 1231
BspEI TCCGGA 1 cut(s) 350
BspHI TCATGA 1 cut(s) 1186
BspLI GGNNCC 7 cut(s) 349, 355, 635, 708, 824, 1116, 1197
BspMI ACCTGC 1 cut(s) 810
BspPI GGATC 3 cut(s) 348, 361, 583
BspT104I TTCGAA 2 cut(s) 653, 870
BspT107I GGYRCC 1 cut(s) 1195
BspTNI GGTCTC 2 cut(s) 545, 1345
BsrBI CCGCTC 1 cut(s) 195
BsrDI GCAATG 1 cut(s) 1058
BsrFI RCCGGY 4 cut(s) 512, 793, 839, 1081
BsrI ACTGG 1 cut(s) 377
BssAI RCCGGY 4 cut(s) 512, 793, 839, 1081
BssECI CCNNGG 5 cut(s) 555, 886, 1064, 1176, 1260
BssMI GATC 2 cut(s) 353, 588
BssT1I CCWWGG 4 cut(s) 555, 886, 1064, 1260
Bst2UI CCWGG 2 cut(s) 169, 1177
Bst4CI ACNGT 1 cut(s) 1013
Bst6I CTCTTC 1 cut(s) 504
BstBI TTCGAA 2 cut(s) 653, 870
BstC8I GCNNGC 8 cut(s) 193, 514, 572, 742, 795, 841, 1083, 1203
BstDEI CTNAG 6 cut(s) 603, 711, 989, 1128, 1218, 1317
BstDSI CCRYGG 2 cut(s) 886, 1064
BstF5I GGATG 4 cut(s) 201, 967, 1138, 1147
BstH2I RGCGCY 1 cut(s) 1088
BstHHI GCGC 2 cut(s) 760, 1087
BstKTI GATC 2 cut(s) 356, 591
BstMAI GTCTC 3 cut(s) 545, 1229, 1345
BstMBI GATC 2 cut(s) 353, 588
BstMCI CGRYCG 1 cut(s) 271
BstNI CCWGG 2 cut(s) 169, 1177
BstSCI CCNGG 4 cut(s) 167, 560, 591, 1175
BstV1I GCAGC 2 cut(s) 1090, 1132
BstX2I RGATCY 1 cut(s) 353
BstXI CCANNNNNNTGG 2 cut(s) 1065, 1267
BstYI RGATCY 1 cut(s) 353
Bsu36I CCTNAGG 1 cut(s) 711
BsuRI GGCC 8 cut(s) 450, 560, 719, 740, 839, 843, 1117, 1259
BtgI CCRYGG 2 cut(s) 886, 1064
BtsCI GGATG 4 cut(s) 201, 967, 1138, 1147
BveI ACCTGC 1 cut(s) 810
Cac8I GCNNGC 8 cut(s) 193, 514, 572, 742, 795, 841, 1083, 1203
CciI TCATGA 1 cut(s) 1186
CfoI GCGC 2 cut(s) 760, 1087
Cfr10I RCCGGY 4 cut(s) 512, 793, 839, 1081
Cfr13I GGNCC 7 cut(s) 4, 559, 595, 738, 801, 837, 1115
CviAII CATG 4 cut(s) 887, 1028, 1065, 1187
DdeI CTNAG 6 cut(s) 603, 711, 989, 1128, 1218, 1317
DpnI GATC 2 cut(s) 355, 590
DpnII GATC 2 cut(s) 353, 588
EaeI YGGCCR 3 cut(s) 717, 841, 1257
Eam1104I CTCTTC 1 cut(s) 504
EarI CTCTTC 1 cut(s) 504
EciI GGCGGA 4 cut(s) 34, 301, 310, 986
Eco130I CCWWGG 4 cut(s) 555, 886, 1064, 1260
Eco24I GRGCYC 1 cut(s) 638
Eco31I GGTCTC 2 cut(s) 545, 1345
Eco47I GGWCC 3 cut(s) 4, 595, 801
Eco81I CCTNAGG 1 cut(s) 711
EcoO109I RGGNCCY 1 cut(s) 1115
EcoRI GAATTC 1 cut(s) 1123
EcoRII CCWGG 2 cut(s) 167, 1175
EcoT14I CCWWGG 4 cut(s) 555, 886, 1064, 1260
EcoT38I GRGCYC 1 cut(s) 638
ErhI CCWWGG 4 cut(s) 555, 886, 1064, 1260
FaeI CATG 4 cut(s) 890, 1031, 1068, 1190
FaiI YATR 6 cut(s) 888, 1029, 1066, 1094, 1173, 1188
FatI CATG 4 cut(s) 886, 1027, 1064, 1186
FauI CCCGC 4 cut(s) 125, 200, 206, 283
FblI GTMKAC 1 cut(s) 728
Fnu4HI GCNGC 8 cut(s) 546, 698, 701, 1079, 1146, 1199, 1257, 1272
FokI GGATG 4 cut(s) 188, 974, 1145, 1154
FriOI GRGCYC 1 cut(s) 638
FseI GGCCGGCC 1 cut(s) 843
Fsp4HI GCNGC 8 cut(s) 546, 698, 701, 1079, 1146, 1199, 1257, 1272
GlaI GCGC 2 cut(s) 759, 1086
GluI GCNGC 8 cut(s) 546, 698, 701, 1079, 1146, 1199, 1257, 1272
GsaI CCCAGC 2 cut(s) 909, 924
GsuI CTGGAG 2 cut(s) 445, 725
HaeII RGCGCY 1 cut(s) 1088
HaeIII GGCC 8 cut(s) 450, 560, 719, 740, 839, 843, 1117, 1259
HhaI GCGC 2 cut(s) 760, 1087
Hin1II CATG 4 cut(s) 890, 1031, 1068, 1190
Hin6I GCGC 2 cut(s) 758, 1085
HinP1I GCGC 2 cut(s) 758, 1085
HincII GTYRAC 3 cut(s) 729, 986, 1300
HindII GTYRAC 3 cut(s) 729, 986, 1300
HpaI GTTAAC 1 cut(s) 1300
HphI GGTGA 4 cut(s) 130, 890, 902, 1171
Hpy166II GTNNAC 3 cut(s) 729, 986, 1300
Hpy188I TCNGA 9 cut(s) 268, 358, 400, 484, 633, 752, 861, 916, 1221
Hpy188III TCNNGA 4 cut(s) 351, 462, 1130, 1187
Hpy8I GTNNAC 3 cut(s) 729, 986, 1300
Hpy99I CGWCG 3 cut(s) 152, 155, 422
HpyAV CCTTC 4 cut(s) 240, 439, 618, 1036
HpyCH4III ACNGT 1 cut(s) 1013
HpyCH4V TGCA 6 cut(s) 163, 369, 676, 744, 819, 1078
HpyF3I CTNAG 6 cut(s) 603, 711, 989, 1128, 1218, 1317
Hsp92II CATG 4 cut(s) 890, 1031, 1068, 1190
HspAI GCGC 2 cut(s) 758, 1085
Kpn2I TCCGGA 1 cut(s) 350
KroI GCCGGC 4 cut(s) 512, 793, 839, 1081
KroNI GCCGGC 4 cut(s) 514, 795, 841, 1083
KspAI GTTAAC 1 cut(s) 1300
Kzo9I GATC 2 cut(s) 353, 588
LmnI GCTCC 6 cut(s) 29, 200, 353, 633, 706, 1069
Lsp1109I GCAGC 2 cut(s) 1090, 1132
LweI GCATC 4 cut(s) 210, 780, 1132, 1190
MalI GATC 2 cut(s) 355, 590
MbiI CCGCTC 1 cut(s) 195
MboI GATC 2 cut(s) 353, 588
MboII GAAGA 2 cut(s) 491, 986
MflI RGATCY 1 cut(s) 353
MhlI GDGCHC 1 cut(s) 638
MluCI AATT 5 cut(s) 866, 993, 1123, 1206, 1282
MlyI GAGTC 6 cut(s) 257, 408, 479, 758, 1046, 1231
MmeI TCCRAC 4 cut(s) 51, 291, 381, 730
MroI TCCGGA 1 cut(s) 350
MroNI GCCGGC 4 cut(s) 512, 793, 839, 1081
MroXI GAANNNNTTC 1 cut(s) 866
MseI TTAA 2 cut(s) 1137, 1299
MslI CAYNNNNRTG 1 cut(s) 1063
MspA1I CMGCKG 1 cut(s) 703
MspR9I CCNGG 4 cut(s) 169, 562, 593, 1177
MvaI CCWGG 2 cut(s) 169, 1177
NaeI GCCGGC 4 cut(s) 514, 795, 841, 1083
NciI CCSGG 2 cut(s) 562, 593
NcoI CCATGG 2 cut(s) 886, 1064
NdeII GATC 2 cut(s) 353, 588
NgoMIV GCCGGC 4 cut(s) 512, 793, 839, 1081
NlaIII CATG 4 cut(s) 890, 1031, 1068, 1190
NlaIV GGNNCC 7 cut(s) 349, 355, 635, 708, 824, 1116, 1197
NmeAIII GCCGAG 2 cut(s) 574, 1332
NspV TTCGAA 2 cut(s) 653, 870
PagI TCATGA 1 cut(s) 1186
PcsI WCGNNNNNNNCGW 2 cut(s) 264, 516
PdiI GCCGGC 4 cut(s) 514, 795, 841, 1083
PdmI GAANNNNTTC 1 cut(s) 866
PfeI GAWTC 4 cut(s) 176, 465, 872, 1054
PfoI TCCNGGA 1 cut(s) 591
PkrI GCNGC 8 cut(s) 547, 699, 702, 1080, 1147, 1200, 1258, 1273
PleI GAGTC 6 cut(s) 257, 408, 479, 758, 1045, 1230
PpsI GAGTC 6 cut(s) 257, 408, 479, 758, 1045, 1230
Psp6I CCWGG 2 cut(s) 167, 1175
PspFI CCCAGC 2 cut(s) 905, 920
PspGI CCWGG 2 cut(s) 167, 1175
PspN4I GGNNCC 7 cut(s) 349, 355, 635, 708, 824, 1116, 1197
PspPI GGNCC 7 cut(s) 4, 559, 595, 738, 801, 837, 1115
PsrI GAACNNNNNNTAC 2 cut(s) 393, 425
PsuI RGATCY 1 cut(s) 353
RigI GGCCGGCC 1 cut(s) 843
RseI CAYNNNNRTG 1 cut(s) 1063
SalI GTCGAC 1 cut(s) 727
SaqAI TTAA 2 cut(s) 1137, 1299
SatI GCNGC 8 cut(s) 546, 698, 701, 1079, 1146, 1199, 1257, 1272
Sau3AI GATC 2 cut(s) 353, 588
Sau96I GGNCC 7 cut(s) 4, 559, 595, 738, 801, 837, 1115
SchI GAGTC 6 cut(s) 257, 408, 479, 758, 1046, 1231
ScrFI CCNGG 4 cut(s) 169, 562, 593, 1177
SduI GDGCHC 1 cut(s) 638
SfaNI GCATC 4 cut(s) 210, 780, 1132, 1190
SfuI TTCGAA 2 cut(s) 653, 870
SinI GGWCC 3 cut(s) 4, 595, 801
SmiMI CAYNNNNRTG 1 cut(s) 1063
Sse9I AATT 5 cut(s) 866, 993, 1123, 1206, 1282
StyD4I CCNGG 4 cut(s) 167, 560, 591, 1175
StyI CCWWGG 4 cut(s) 555, 886, 1064, 1260
TaaI ACNGT 1 cut(s) 1013
TaqI TCGA 7 cut(s) 519, 587, 653, 659, 728, 870, 1040
TasI AATT 5 cut(s) 866, 993, 1123, 1206, 1282
TauI GCSGC 6 cut(s) 548, 700, 703, 1201, 1259, 1274
TfiI GAWTC 4 cut(s) 176, 465, 872, 1054
Tru1I TTAA 2 cut(s) 1137, 1299
Tru9I TTAA 2 cut(s) 1137, 1299
TseI GCWGC 2 cut(s) 1078, 1145
VpaK11BI GGWCC 3 cut(s) 4, 595, 801
XapI RAATTY 1 cut(s) 1123
XcmI CCANNNNNNNNNTGG 1 cut(s) 165
XmiI GTMKAC 1 cut(s) 728
XmnI GAANNNNTTC 1 cut(s) 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.