Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009339

Physical Location & Seq

Genomic Coordinates Reverse (-)

25816918 .. 25821567

Gene Structure 4650 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G64580.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2568 bp

ATGACGTTTTATATCTCGAGCTCGTTAACTCCAACCCATTTCTCGAAACCCTTAAACCCTTCTAATACCCTCTTCCCCAGTCAATTTCGAGGTTCTCTGTCTAGTTTCGTCCGTCGAAGAAAACCAACGGAAGCTAAATTAAGCTCCAAATTCAATCTTTTTCCTTCACGAAGAAATGGTTTGATTACTTGTTGCAGTACATCGAGCTTTGAGTCTACTGAGTCTTCAGTTTCACAGGAAGAAGATGCTGAGTCCAATCGTCTATTTGAGAAGTTACGAGAAACTGAAAGAGAACGACTTAGCAACATGGAAGAGCTCGAGAGAAAGGCTAATGTACAATTAGAAAGGCAGCTTGTTATGGCATCAGATTGGAGTAGAACATTGTTAACCATGCGTGGCAAGTTAAAAGGAACAGAATGGGATCCTGAAACCTCTCACCGTATCAATTTTAGTGATTTTATGAAGCTCTTAGATTCCAACAGTGTTCAATACATGGAATATTCAAATTACGGACAGACGATATCAGTGATATTGCCTTATTATAAAGATGGGGAGCCCCTAGGAGAAGAAGAAGATTCGAAAAAGGAGATAATTTTCCGTCGTCACATTGTGGACCGAATGCCAATTGATGGTTGGAATGATGTATGGAAGAAGTTGCATCAGCAGATAGTAAACGTAGAGGTTTTCAATGTAGATGTGGTTCCAGCTGAAGTCTATACTACAGTTGCCACTTTTGTGGTTTGGTCTATGAGACTTGCCCTCTTTGTTTCGCTTTATGTTTGGATCGACAGTATCACGAGGCCAATCTATGCCAAACTAATCCCTTGTGATCTCGGAACCCCTACCAAAAAGATAAGGCAGCCCCTCAAGCGCCAAGCACTCGGTTCCCTAGGCAAGAGTCGGGCAAAATTTATATCAGCAGAGGAGAAAACTGGTGTTACTTTTGATGATTTTGCGGGTCAGGAGTATATAAAGAGGGAATTACAAGAAATTGTGCGAATTTTAAAGAATGATGAGGAATTTCAGAATAAAGGCATTTATTGTCCGAAAGGTGTTCTGCTTCATGGACCTCCCGGAACGGGGAAAACTTTGTTAGCCAAAGCTATTGCTGGAGAAGCTGGTTTACCTTTCTTCGCGGCAAATGGAACCGACTTTGTTGAGATGTTTGTAGGAGTGGCTGCATCTCGTGTTAAAGATCTTTTTGCTAGCTCTCGGTCATATGCGCCATCTATAATCTTCATTGATGAGATTGATGCAATTGGAAGTAAGCGAGGTGGACCCGATATTGGCGGGGGAGGAGCAGAGAGAGAACAAGGTTTGCTTCAAATATTGACTGAGATGGATGGATTTAAAGTTACTACATCACAAGTATTAGTCATAGGTGCAACAAATAGATTAGATATCCTTGATCCTGCTCTATTGAGAAAAGGTCGTTTTGACAAAATCATAAGAGTTGGTTTACCGTCAAAAGATGGTAGATTGGCGATATTAAAGGTACATGCTAGAAATAAATTCTTTCGATCTGAGGATGAGAAGGAGGAGTTGCTGCAAGAAGTAGCTGAGAACACGGAAGATTTTACCGGAGCAGAACTGCAAAACGTTCTAAATGAAGCTGGAATCTTGACAGCTAGAAAAGATTTGGATTACATTGGAAGAGAAGAACTCCTTGAGGCGCTTAAGCGGCAAAAAGGGACATTTGAGACTGGACAAGAAGATAGTACTGAAGTTCCAGAGGAACTGAAGTTGAGATTGGCTTACCGAGAAGCAGCTGTTGCTGTTCTTGCTTGTTATCTCCCAGATCAATATCGTCCAATCAGCGAGACTGACATAAACTCGATTAGAAGCCAACCAAATATGCGTTACTCTGAAACTTCAGGAAGAGTTTTTGCGAGAAAGTCAGATTATGTGAACTCAATAATCCGTGCATGTGCTCCTAGAGTAGTTGAAGAAGAGATGTTTGGAATAGAAAACCTGTGTTGGATCTCTGCTAAGTCTACTTTAGAAGCTTCACAGCGAGCGGAATTTCTGATTCTTCAGACGGGAATGACTGCTTTTGGAAAAGCTTATTACCGAAACCAAAGAGACCTCGTTCCTAATCTTGTTCCGAAACTTGAGGCATTACGCGATGAGTACATGAGGTTTGCTGTTGAGAAATGCTCATCAATCTTACAAGAGTATCAATCAGCCCTTGAAGAAATCACAGATGTGCTTCTTGAGAAAGGAGAAATCAAAGCCGATGAAATCTGGAACATTTACAACACAGCTCCAAGGATTCCTCAAAAACCCGTGAGACCTGTCGATGAGTATGGAGCTTTAATCTATGCGGGAAGATGGGGAATTCACGGTGTATCGCTTCCTGGAAGAGTAACATTTTCACCAGGAAACATTGGTTTTGCAACATTTGGAGCTCCTCGTCCCATGGAGACACAAATCATCAGCGATGACACATGGAAACTAGTGGATGAGATTTGGGATAAAAAGGTTGAAGAAATCAAAGCAGAAGCTGTTATTCAAATTGAAGAAGAAAAGAAGAAACCTCAGATTTTGATGGCAACCCATTTCTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009790 GO:0009791 GO:0009793 GO:0009941 GO:0010154 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0022414 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0032501 GO:0032502 GO:0034357 GO:0042623 GO:0042651 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0055035 GO:0061458 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA

Protein Analysis

855

Amino Acids

96.85

Weight (kDa)

5.67

Isoelectric Point (pI)

47.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RuvB_N	PF05496	314 - 419	9.4e-06	Holliday junction DNA helicase RuvB P-loop domain
AAA	PF00004	352 - 486	3e-41	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	516 - 555	1.1e-08	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009339)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64580
fragaria_vesca	FvH4_1g24180 FvH4_1g24180 FvH4_1g24180 FvH4_1g24180 FvH4_1g24180
malus_domestica	MD02G1315900.v1.1 MD07G1006000.v1.1
prunus_persica	Prupe.1G190200_v2.0.a1 Prupe.2G008800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0120571
rosa_laevigata	RLG00000018562
rosa_multiflora	Rmu_co8081184.1_g000001 Rmu_sc0001844.1_g000028 Rmu_sc0007380.1_g000017
rosa_roxburghii	Rroxscaffold_2G00123290
rosa_rugosa	Rorug02G0229500
rosa_samantha	Rh2AG285600 Rh2BG297200 Rh2CG274800 Rh2DG310900
rosa_wichuraiana	Rw2G022920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	543
AccB7I	CCANNNNNTGG	1 cut(s)	629
AccBSI	CCGCTC	1 cut(s)	2018
AccI	GTMKAC	2 cut(s)	215, 1994
AccII	CGCG	2 cut(s)	1136, 2124
AciI	CCGC	6 cut(s)	956, 1136, 1290, 1681, 2018, 2324
AclI	AACGTT	1 cut(s)	1599
AclWI	GGATC	5 cut(s)	416, 429, 791, 1403, 1988
AcsI	RAATTY	7 cut(s)	149, 908, 999, 1019, 1511, 2021, 2337
AcuI	CTGAAG	6 cut(s)	210, 729, 1743, 1760, 1857, 2018
AdeI	CACNNNGTG	1 cut(s)	610
AfaI	GTAC	5 cut(s)	199, 336, 1497, 1720, 2132
AfiI	CCNNNNNNNGG	4 cut(s)	629, 1079, 1080, 1286
AflII	CTTAAG	1 cut(s)	1676
AhlI	ACTAGT	1 cut(s)	2455
AjnI	CCWGG	2 cut(s)	2356, 2377
AjuI	GAANNNNNNNTTGG	4 cut(s)	1733, 1765, 1941, 1973
AleI	CACNNNNGTG	2 cut(s)	734, 2204
Alw21I	GWGCWC	4 cut(s)	23, 318, 1933, 2410
Alw26I	GTCTC	6 cut(s)	745, 1694, 1814, 2076, 2284, 2417
AlwI	GGATC	5 cut(s)	416, 429, 791, 1403, 1988
AlwNI	CAGNNNCTG	1 cut(s)	2504
Ama87I	CYCGRG	2 cut(s)	16, 317
AoxI	GGCC	1 cut(s)	800
ApeKI	GCWGC	5 cut(s)	349, 859, 1178, 1546, 1766
ApoI	RAATTY	7 cut(s)	149, 908, 999, 1019, 1511, 2021, 2337
AspA2I	CCTAGG	2 cut(s)	559, 889
AspLEI	GCGC	3 cut(s)	873, 1225, 1675
AspS9I	GGNCC	3 cut(s)	613, 1067, 1277
AsuC2I	CCSGG	1 cut(s)	1074
AsuHPI	GGTGA	2 cut(s)	428, 2367
AsuII	TTCGAA	1 cut(s)	578
AsuNHI	GCTAGC	1 cut(s)	1205
AvaI	CYCGRG	2 cut(s)	16, 317
AvaII	GGWCC	3 cut(s)	613, 1067, 1277
AvrII	CCTAGG	2 cut(s)	559, 889
BaeI	ACNNNNGTAYC	2 cut(s)	2159, 2192
BamHI	GGATCC	1 cut(s)	421
BanII	GRGCYC	4 cut(s)	23, 318, 558, 2410
BarI	GAAGNNNNNNTAC	2 cut(s)	1107, 1139
BauI	CACGAG	2 cut(s)	796, 1185
BbsI	GAAGAC	1 cut(s)	216
Bbv12I	GWGCWC	4 cut(s)	23, 318, 1933, 2410
BbvI	GCAGC	5 cut(s)	361, 871, 1165, 1533, 1778
BccI	CCATC	8 cut(s)	542, 623, 1234, 1333, 1337, 1466, 2325, 2542
BciT130I	CCWGG	2 cut(s)	2358, 2379
BcnI	CCSGG	1 cut(s)	1074
BcoDI	GTCTC	6 cut(s)	745, 1694, 1814, 2076, 2284, 2417
BcuI	ACTAGT	1 cut(s)	2455
BfaI	CTAG	8 cut(s)	102, 560, 890, 1206, 1503, 1629, 1935, 2456
BfmI	CTRYAG	1 cut(s)	720
BfoI	RGCGCY	2 cut(s)	874, 1676
BfrI	CTTAAG	1 cut(s)	1676
BglII	AGATCT	1 cut(s)	1195
BisI	GCNGC	7 cut(s)	350, 860, 1137, 1179, 1547, 1682, 1767
BlnI	CCTAGG	2 cut(s)	559, 889
BlsI	GCNGC	7 cut(s)	351, 861, 1138, 1180, 1548, 1683, 1768
BmcAI	AGTACT	1 cut(s)	1720
Bme1390I	CCNGG	3 cut(s)	1074, 2358, 2379
Bme18I	GGWCC	3 cut(s)	613, 1067, 1277
BmeT110I	CYCGRG	2 cut(s)	16, 317
BmgT120I	GGNCC	3 cut(s)	613, 1067, 1277
BmiI	GGNNCC	7 cut(s)	423, 555, 702, 838, 886, 1147, 1279
BmrFI	CCNGG	3 cut(s)	1074, 2358, 2379
BmrI	ACTGGG	1 cut(s)	72
BmsI	GCATC	5 cut(s)	235, 371, 667, 1190, 1243
BmtI	GCTAGC	1 cut(s)	1209
BmuI	ACTGGG	1 cut(s)	72
BpiI	GAAGAC	1 cut(s)	216
BplI	GAGNNNNNCTC	4 cut(s)	1647, 1679, 2141, 2173
BpmI	CTGGAG	1 cut(s)	1131
Bpu14I	TTCGAA	1 cut(s)	578
BpuEI	CTTGAG	4 cut(s)	851, 1688, 2132, 2234
BpuMI	CCSGG	1 cut(s)	1074
BsaBI	GATNNNNATC	1 cut(s)	1803
BsaI	GGTCTC	2 cut(s)	2076, 2284
BsaJI	CCNNGG	4 cut(s)	559, 889, 2267, 2418
BsaWI	WCCGGW	1 cut(s)	1580
Bsc4I	CCNNNNNNNGG	4 cut(s)	629, 1079, 1080, 1286
Bse1I	ACTGG	3 cut(s)	78, 937, 1708
Bse8I	GATNNNNATC	1 cut(s)	1803
BseBI	CCWGG	2 cut(s)	2358, 2379
BseDI	CCNNGG	4 cut(s)	559, 889, 2267, 2418
BseGI	GGATG	3 cut(s)	1348, 1534, 2467
BseJI	GATNNNNATC	1 cut(s)	1803
BseLI	CCNNNNNNNGG	4 cut(s)	629, 1079, 1080, 1286
BseMII	CTCAG	6 cut(s)	210, 240, 1326, 1515, 1551, 2552
BseNI	ACTGG	3 cut(s)	78, 937, 1708
BseRI	GAGGAG	4 cut(s)	938, 1311, 1553, 2400
BseXI	GCAGC	5 cut(s)	361, 871, 1165, 1533, 1778
Bsh1236I	CGCG	2 cut(s)	1136, 2124
BshFI	GGCC	1 cut(s)	802
BsiHKAI	GWGCWC	4 cut(s)	23, 318, 1933, 2410
BsiHKCI	CYCGRG	2 cut(s)	16, 317
BsiSI	CCGG	2 cut(s)	1074, 1581
BslFI	GGGAC	2 cut(s)	1705, 2400
BslI	CCNNNNNNNGG	4 cut(s)	629, 1079, 1080, 1286
BsmAI	GTCTC	6 cut(s)	745, 1694, 1814, 2076, 2284, 2417
BsmFI	GGGAC	2 cut(s)	1705, 2400
BsmI	GAATGC	1 cut(s)	624
BsnI	GGCC	1 cut(s)	802
Bso31I	GGTCTC	2 cut(s)	2076, 2284
BsoBI	CYCGRG	2 cut(s)	16, 317
Bsp119I	TTCGAA	1 cut(s)	578
Bsp1286I	GDGCHC	5 cut(s)	23, 318, 558, 1933, 2410
Bsp1407I	TGTACA	1 cut(s)	334
Bsp143I	GATC	8 cut(s)	421, 783, 829, 1195, 1408, 1520, 1798, 1980
Bsp19I	CCATGG	1 cut(s)	2418
BspACI	CCGC	6 cut(s)	956, 1136, 1290, 1681, 2018, 2324
BspANI	GGCC	1 cut(s)	802
BspCNI	CTCAG	6 cut(s)	211, 241, 1327, 1516, 1552, 2551
BspFNI	CGCG	2 cut(s)	1136, 2124
BspLI	GGNNCC	7 cut(s)	423, 555, 702, 838, 886, 1147, 1279
BspOI	GCTAGC	1 cut(s)	1209
BspPI	GGATC	5 cut(s)	416, 429, 791, 1403, 1988
BspQI	GCTCTTC	1 cut(s)	306
BspT104I	TTCGAA	1 cut(s)	578
BspTI	CTTAAG	1 cut(s)	1676
BspTNI	GGTCTC	2 cut(s)	2076, 2284
BsrBI	CCGCTC	1 cut(s)	2018
BsrGI	TGTACA	1 cut(s)	334
BsrI	ACTGG	3 cut(s)	78, 937, 1708
BssECI	CCNNGG	4 cut(s)	559, 889, 2267, 2418
BssMI	GATC	8 cut(s)	421, 783, 829, 1195, 1408, 1520, 1798, 1980
BssSI	CACGAG	2 cut(s)	796, 1185
BssT1I	CCWWGG	4 cut(s)	559, 889, 2267, 2418
Bst2BI	CACGAG	2 cut(s)	796, 1185
Bst2UI	CCWGG	2 cut(s)	2358, 2379
Bst4CI	ACNGT	6 cut(s)	440, 482, 724, 791, 1464, 2345
Bst6I	CTCTTC	6 cut(s)	77, 306, 1648, 1873, 1944, 2356
BstAFI	CTTAAG	1 cut(s)	1676
BstAPI	GCANNNNNTGC	1 cut(s)	1772
BstAUI	TGTACA	1 cut(s)	334
BstBI	TTCGAA	1 cut(s)	578
BstC8I	GCNNGC	2 cut(s)	1207, 2016
BstDEI	CTNAG	9 cut(s)	219, 249, 299, 469, 1335, 1524, 1560, 1989, 2538
BstDSI	CCRYGG	1 cut(s)	2418
BstF5I	GGATG	3 cut(s)	1348, 1534, 2467
BstFNI	CGCG	2 cut(s)	1136, 2124
BstH2I	RGCGCY	2 cut(s)	874, 1676
BstHHI	GCGC	3 cut(s)	873, 1225, 1675
BstKTI	GATC	8 cut(s)	424, 786, 832, 1198, 1411, 1523, 1801, 1983
BstMAI	GTCTC	6 cut(s)	745, 1694, 1814, 2076, 2284, 2417
BstMBI	GATC	8 cut(s)	421, 783, 829, 1195, 1408, 1520, 1798, 1980
BstMWI	GCNNNNNNNGC	5 cut(s)	868, 1115, 1681, 1772, 1781
BstNI	CCWGG	2 cut(s)	2358, 2379
BstNSI	RCATGY	2 cut(s)	1502, 1929
BstSCI	CCNGG	3 cut(s)	1072, 2356, 2377
BstSFI	CTRYAG	1 cut(s)	720
BstUI	CGCG	2 cut(s)	1136, 2124
BstV1I	GCAGC	5 cut(s)	361, 871, 1165, 1533, 1778
BstV2I	GAAGAC	1 cut(s)	216
BstX2I	RGATCY	3 cut(s)	421, 1195, 1980
BstXI	CCANNNNNNTGG	1 cut(s)	736
BstYI	RGATCY	3 cut(s)	421, 1195, 1980
BsuRI	GGCC	1 cut(s)	802
BtgI	CCRYGG	1 cut(s)	2418
BtgZI	GCGATG	2 cut(s)	2139, 2454
BtsCI	GGATG	3 cut(s)	1348, 1534, 2467
BtsIMutI	CAGTG	2 cut(s)	487, 531
Cac8I	GCNNGC	2 cut(s)	1207, 2016
CaiI	CAGNNNCTG	1 cut(s)	2504
CfoI	GCGC	3 cut(s)	873, 1225, 1675
Cfr13I	GGNCC	3 cut(s)	613, 1067, 1277
Csp6I	GTAC	5 cut(s)	198, 335, 1496, 1719, 2131
CviAII	CATG	9 cut(s)	307, 391, 493, 1064, 1499, 1926, 2134, 2419, 2448
CviQI	GTAC	5 cut(s)	198, 335, 1496, 1719, 2131
DdeI	CTNAG	9 cut(s)	219, 249, 299, 469, 1335, 1524, 1560, 1989, 2538
DpnI	GATC	8 cut(s)	423, 785, 831, 1197, 1410, 1522, 1800, 1982
DpnII	GATC	8 cut(s)	421, 783, 829, 1195, 1408, 1520, 1798, 1980
DraI	TTTAAA	2 cut(s)	1005, 1351
DraIII	CACNNNGTG	1 cut(s)	610
Eam1104I	CTCTTC	6 cut(s)	77, 306, 1648, 1873, 1944, 2356
EarI	CTCTTC	6 cut(s)	77, 306, 1648, 1873, 1944, 2356
Ecl136II	GAGCTC	3 cut(s)	21, 316, 2408
Eco130I	CCWWGG	4 cut(s)	559, 889, 2267, 2418
Eco24I	GRGCYC	4 cut(s)	23, 318, 558, 2410
Eco31I	GGTCTC	2 cut(s)	2076, 2284
Eco32I	GATATC	2 cut(s)	522, 1402
Eco47I	GGWCC	3 cut(s)	613, 1067, 1277
Eco53kI	GAGCTC	3 cut(s)	21, 316, 2408
Eco57I	CTGAAG	6 cut(s)	210, 729, 1743, 1760, 1857, 2018
Eco88I	CYCGRG	2 cut(s)	16, 317
EcoICRI	GAGCTC	3 cut(s)	21, 316, 2408
EcoRI	GAATTC	1 cut(s)	2337
EcoRII	CCWGG	2 cut(s)	2356, 2377
EcoRV	GATATC	2 cut(s)	522, 1402
EcoT14I	CCWWGG	4 cut(s)	559, 889, 2267, 2418
EcoT38I	GRGCYC	4 cut(s)	23, 318, 558, 2410
ErhI	CCWWGG	4 cut(s)	559, 889, 2267, 2418
FaeI	CATG	9 cut(s)	310, 394, 496, 1067, 1502, 1929, 2137, 2422, 2451
FalI	AAGNNNNNCTT	4 cut(s)	1305, 1337, 1650, 1682
FaqI	GGGAC	2 cut(s)	1705, 2400
FatI	CATG	9 cut(s)	306, 390, 492, 1063, 1498, 1925, 2133, 2418, 2447
FauI	CCCGC	3 cut(s)	949, 1283, 2317
FauNDI	CATATG	1 cut(s)	1219
FblI	GTMKAC	2 cut(s)	215, 1994
Fnu4HI	GCNGC	7 cut(s)	350, 860, 1137, 1179, 1547, 1682, 1767
FokI	GGATG	3 cut(s)	1355, 1541, 2474
FriOI	GRGCYC	4 cut(s)	23, 318, 558, 2410
Fsp4HI	GCNGC	7 cut(s)	350, 860, 1137, 1179, 1547, 1682, 1767
FspBI	CTAG	8 cut(s)	102, 560, 890, 1206, 1503, 1629, 1935, 2456
GlaI	GCGC	3 cut(s)	872, 1224, 1674
GluI	GCNGC	7 cut(s)	350, 860, 1137, 1179, 1547, 1682, 1767
GsuI	CTGGAG	1 cut(s)	1131
HaeII	RGCGCY	2 cut(s)	874, 1676
HaeIII	GGCC	1 cut(s)	802
HapII	CCGG	2 cut(s)	1074, 1581
HhaI	GCGC	3 cut(s)	873, 1225, 1675
Hin1II	CATG	9 cut(s)	310, 394, 496, 1067, 1502, 1929, 2137, 2422, 2451
Hin6I	GCGC	3 cut(s)	871, 1223, 1673
HinP1I	GCGC	3 cut(s)	871, 1223, 1673
HincII	GTYRAC	2 cut(s)	27, 387
HindII	GTYRAC	2 cut(s)	27, 387
HindIII	AAGCTT	2 cut(s)	2004, 2061
HinfI	GANTC	9 cut(s)	212, 221, 251, 473, 575, 898, 1617, 2029, 2272
HpaI	GTTAAC	2 cut(s)	27, 387
HpaII	CCGG	2 cut(s)	1074, 1581
HphI	GGTGA	2 cut(s)	428, 2367
Hpy99I	CGWCG	2 cut(s)	117, 603
HpyAV	CCTTC	3 cut(s)	69, 174, 1528
HpyCH4III	ACNGT	6 cut(s)	440, 482, 724, 791, 1464, 2345
HpyCH4IV	ACGT	3 cut(s)	5, 675, 1599
HpyCH4V	TGCA	9 cut(s)	195, 658, 1181, 1256, 1385, 1549, 1594, 1925, 2396
HpyF10VI	GCNNNNNNNGC	5 cut(s)	868, 1115, 1681, 1772, 1781
HpyF3I	CTNAG	9 cut(s)	219, 249, 299, 469, 1335, 1524, 1560, 1989, 2538
HpySE526I	ACGT	3 cut(s)	5, 675, 1599
Hsp92II	CATG	9 cut(s)	310, 394, 496, 1067, 1502, 1929, 2137, 2422, 2451
HspAI	GCGC	3 cut(s)	871, 1223, 1673
KspAI	GTTAAC	2 cut(s)	27, 387
Kzo9I	GATC	8 cut(s)	421, 783, 829, 1195, 1408, 1520, 1798, 1980
LguI	GCTCTTC	1 cut(s)	306
LmnI	GCTCC	9 cut(s)	149, 553, 1298, 1583, 1936, 2269, 2309, 2405, 2413
Lsp1109I	GCAGC	5 cut(s)	361, 871, 1165, 1533, 1778
LweI	GCATC	5 cut(s)	235, 371, 667, 1190, 1243
MaeI	CTAG	8 cut(s)	102, 560, 890, 1206, 1503, 1629, 1935, 2456
MaeII	ACGT	3 cut(s)	5, 675, 1599
MaeIII	GTNAC	6 cut(s)	273, 602, 937, 1354, 1859, 2365
MalI	GATC	8 cut(s)	423, 785, 831, 1197, 1410, 1522, 1800, 1982
MbiI	CCGCTC	1 cut(s)	2018
MboI	GATC	8 cut(s)	421, 783, 829, 1195, 1408, 1520, 1798, 1980
MfeI	CAATTG	2 cut(s)	624, 1257
MflI	RGATCY	3 cut(s)	421, 1195, 1980
MhlI	GDGCHC	5 cut(s)	23, 318, 558, 1933, 2410
MlyI	GAGTC	4 cut(s)	221, 230, 260, 907
MmeI	TCCRAC	4 cut(s)	56, 501, 614, 1958
MslI	CAYNNNNRTG	2 cut(s)	734, 2204
MspA1I	CMGCKG	2 cut(s)	707, 1769
MspCI	CTTAAG	1 cut(s)	1676
MspI	CCGG	2 cut(s)	1074, 1581
MspR9I	CCNGG	3 cut(s)	1074, 2358, 2379
MunI	CAATTG	2 cut(s)	624, 1257
Mva1269I	GAATGC	1 cut(s)	624
MvaI	CCWGG	2 cut(s)	2358, 2379
MvnI	CGCG	2 cut(s)	1136, 2124
MwoI	GCNNNNNNNGC	5 cut(s)	868, 1115, 1681, 1772, 1781
NciI	CCSGG	1 cut(s)	1074
NcoI	CCATGG	1 cut(s)	2418
NdeI	CATATG	1 cut(s)	1219
NdeII	GATC	8 cut(s)	421, 783, 829, 1195, 1408, 1520, 1798, 1980
NheI	GCTAGC	1 cut(s)	1205
NlaIII	CATG	9 cut(s)	310, 394, 496, 1067, 1502, 1929, 2137, 2422, 2451
NlaIV	GGNNCC	7 cut(s)	423, 555, 702, 838, 886, 1147, 1279
NmuCI	GTSAC	1 cut(s)	602
NspI	RCATGY	2 cut(s)	1502, 1929
NspV	TTCGAA	1 cut(s)	578
OliI	CACNNNNGTG	2 cut(s)	734, 2204
PaeR7I	CTCGAG	2 cut(s)	16, 317
PciSI	GCTCTTC	1 cut(s)	306
PctI	GAATGC	1 cut(s)	624
PfeI	GAWTC	5 cut(s)	473, 575, 1617, 2029, 2272
PflMI	CCANNNNNTGG	1 cut(s)	629
PfoI	TCCNGGA	2 cut(s)	1072, 2356
PkrI	GCNGC	7 cut(s)	351, 861, 1138, 1180, 1548, 1683, 1768
PleI	GAGTC	4 cut(s)	220, 229, 259, 906
PpsI	GAGTC	4 cut(s)	220, 229, 259, 906
PsiI	TTATAA	1 cut(s)	543
Psp124BI	GAGCTC	3 cut(s)	23, 318, 2410
Psp1406I	AACGTT	1 cut(s)	1599
Psp6I	CCWGG	2 cut(s)	2356, 2377
PspGI	CCWGG	2 cut(s)	2356, 2377
PspN4I	GGNNCC	7 cut(s)	423, 555, 702, 838, 886, 1147, 1279
PspPI	GGNCC	3 cut(s)	613, 1067, 1277
PsrI	GAACNNNNNNTAC	2 cut(s)	684, 716
PstNI	CAGNNNCTG	1 cut(s)	2504
PsuI	RGATCY	3 cut(s)	421, 1195, 1980
PvuII	CAGCTG	2 cut(s)	707, 1769
RsaI	GTAC	5 cut(s)	199, 336, 1497, 1720, 2132
RsaNI	GTAC	5 cut(s)	198, 335, 1496, 1719, 2131
RseI	CAYNNNNRTG	2 cut(s)	734, 2204
SacI	GAGCTC	3 cut(s)	23, 318, 2410
SapI	GCTCTTC	1 cut(s)	306
SatI	GCNGC	7 cut(s)	350, 860, 1137, 1179, 1547, 1682, 1767
Sau3AI	GATC	8 cut(s)	421, 783, 829, 1195, 1408, 1520, 1798, 1980
Sau96I	GGNCC	3 cut(s)	613, 1067, 1277
ScaI	AGTACT	1 cut(s)	1720
SchI	GAGTC	4 cut(s)	221, 230, 260, 907
ScrFI	CCNGG	3 cut(s)	1074, 2358, 2379
SduI	GDGCHC	5 cut(s)	23, 318, 558, 1933, 2410
SfaNI	GCATC	5 cut(s)	235, 371, 667, 1190, 1243
SfcI	CTRYAG	1 cut(s)	720
Sfr274I	CTCGAG	2 cut(s)	16, 317
SfuI	TTCGAA	1 cut(s)	578
SinI	GGWCC	3 cut(s)	613, 1067, 1277
SlaI	CTCGAG	2 cut(s)	16, 317
SmiMI	CAYNNNNRTG	2 cut(s)	734, 2204
SmlI	CTYRAG	7 cut(s)	16, 317, 866, 1667, 1676, 2111, 2213
SmoI	CTYRAG	7 cut(s)	16, 317, 866, 1667, 1676, 2111, 2213
SpeI	ACTAGT	1 cut(s)	2455
SsiI	CCGC	6 cut(s)	956, 1136, 1290, 1681, 2018, 2324
SspI	AATATT	2 cut(s)	500, 1329
SspMI	CTAG	8 cut(s)	102, 560, 890, 1206, 1503, 1629, 1935, 2456
SstI	GAGCTC	3 cut(s)	23, 318, 2410
StyD4I	CCNGG	3 cut(s)	1072, 2356, 2377
StyI	CCWWGG	4 cut(s)	559, 889, 2267, 2418
TaaI	ACNGT	6 cut(s)	440, 482, 724, 791, 1464, 2345
TaiI	ACGT	3 cut(s)	8, 678, 1602
TaqII	GACCGA	2 cut(s)	630, 1203
TatI	WGTACW	4 cut(s)	197, 334, 1718, 2130
TauI	GCSGC	2 cut(s)	1139, 1684
TfiI	GAWTC	5 cut(s)	473, 575, 1617, 2029, 2272
TscAI	CASTG	2 cut(s)	487, 531
TseFI	GTSAC	1 cut(s)	602
TseI	GCWGC	5 cut(s)	349, 859, 1178, 1546, 1766
Tsp45I	GTSAC	1 cut(s)	602
TspDTI	ATGAA	5 cut(s)	476, 1052, 1228, 1623, 2253
TspGWI	ACGGA	6 cut(s)	101, 143, 525, 587, 1583, 1910
TspRI	CASTG	2 cut(s)	487, 531
Van91I	CCANNNNNTGG	1 cut(s)	629
Vha464I	CTTAAG	1 cut(s)	1676
VpaK11BI	GGWCC	3 cut(s)	613, 1067, 1277
XapI	RAATTY	7 cut(s)	149, 908, 999, 1019, 1511, 2021, 2337
XceI	RCATGY	2 cut(s)	1502, 1929
XcmI	CCANNNNNNNNNTGG	1 cut(s)	630
XhoI	CTCGAG	2 cut(s)	16, 317
XmaJI	CCTAGG	2 cut(s)	559, 889
XmiI	GTMKAC	2 cut(s)	215, 1994
XspI	CTAG	8 cut(s)	102, 560, 890, 1206, 1503, 1629, 1935, 2456
ZrmI	AGTACT	1 cut(s)	1720

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.