Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0009339

Physical Location & Seq

Genomic Coordinates Forward (+)

57152240 .. 57164938

Gene Structure 12699 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00123290.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2517 bp

ATGAAATCCCTACTGGTCTTAAACCCACTTCCCTTGAGCCCTAAAACCCTTGCTCTCCAATTCCCACATCCTTCAATTACCAACCATGGCTGCTCTTTCAGAACCATAAAGCACGGCGCCATTTCTCTCAGGCAGCTCAGAATTCGCTCCGCTGCTTCCAACTCAGTTGCAGCTTTCGCTAACGCCGACGAAGAAGCCGAGTCCGCTCAGCTCTTTGAGAAATTGAAGGATGCAGAGAGACAAAGGATAAATGATTTGGAAGAACTTGAGAAGAAGGCAAATATACAGTTAGAGAGGCAGCTTGTCATGGCTTCATATTGGAGCAGGGCCTTATTGACTATGCGTGGCAAGTTAAGGGGGACAGAGTGGGATCCTGAAAATTCACATAGGATTAATTTTAGTGACTTTTTGAGACTGCTTAACTCAAACAATGTCCAGTTTATGGAGTATTCAAACTATGGTCAAACAATATCAGTGATCCTACCTTACTACAAAGATGAGAAAATGGGAGGAGCGGATGGGAATTCAAAAAAGGAAATTATTTTTAGGCGTCACGTGGTTGACCGAATGCCAATAGATTGTTGGAATGATGTTTGGCAGAAGTTGCATCAGCAAATAGTAAATGTTGAAGTTTATAATGTGGATACAGTACCTGCAGAAGTCTATTCTACTGTTGCAACTGCAGTCATATGGTCCATGCGGCTTGCCTTGTCTATTGTATTGTATCTGTGGATTGATAACATGATGAGACCGATTTATGCGAAGTTGATACCTTCTGATTTAGGAACTCCTAGCAAAAAAACCAGGCAACCACTCAAGCGCCGTGCCCTTGGATCATTAGGAAAGAGCCGGGCCAAATTTATATCTGCAGAAGAATCTACTGGGATTACCTTTGATGATTTTGCTGGCCAGGAGTATATAAAGAGGGAGCTGCAAGAGATTGTACGGATTTTAAAGAATGATGAAGAGTTTCAGGACAAAGGCATCTATTGCCCGAAAGGTGTACTTCTCCATGGACCTCCTGGAACTGGTAAAACACTGCTGGCTAAAGCTATTGCTGGTGAAGCAGGCCTGCCATTCTTTGCAGCAAATGGCACTGATTTTGTAGAGATGTTTGTTGGTGTTGCAGCATCTCGTGTGAAGGATCTATTTGCTAGTGCCAGATCCTTCACACCTTCCATTATATTTATTGATGAGATAGATGCCATTGGTAGCAAACGTGGTGGACCGGATATTGGTGGGGGTGGCGCAGAACGAGAACAAGGCCTGCTTCAGATACTGACAGAGATGGATGGATTCAAAGTAGCTACCTCACAGGTGCTGGTTATAGGTGCAACAAATAGGCTAGATATCCTAGATCCTGCTCTATTGAGGAAGGGTCGTTTTGACAAGATTATAAGAGTTGGTTTGCCATCAAAGGATGGTAGATATGCCATATTGAAGGTGCATGCAAGGAATAAATTTTTCCGTTCTGAAGAGGAGAAGGAGACTCTTCTTCAGGAAATTGCGGAACTTACAGAAGATTTTACTGGAGCAGAGCTGCAGAACATACTGAACGAAGCTGGTATTTTGACTGCCAGGAAGGACTTAGATTACATTGGACGTGAAGAGCTACTAGAGGCTTTGAAAAGGCAAAAGGGCACTTTTGAAACCGGCCAAGAAGACAGTACTGCAATGCCAGAAGAATTAAAACTGAGATTGGCATATAGAGAAGCAGCTGTTGCTGTCCTGGCATGCTACTTTCCAGATCCATATCGTCCTTTCATTGAGACAGATATCAAATCCATTAATAGTCAGCCCAATATGCGCTACACTGAAATATCTGGCAAGGTCTTCTCAAGAAAATCAGATTTCGTAAACGCAATAGTGTGTGCATGTGCTCCTAGAGTAATTGAGGAGGAGATGTTTGGGGTTGACAATTTGTGTTGGATCTCTGCAAAAGCTACATTGGAAGCCTCGAGGCGTGCAGAATTTCTGATTCTTCAGACAGGAATGACAGCATACGGGAAAGCATACTACAGAAATCAAAGTGATCTTGTGCCAAATCTTGCAGCTAAGCTTGAAGCACTTCGTGATGAATATATGCGTTATGCTGTTGACAAGTGTTCATCCGTGTTAAGGGAGTACCATTCAGCTGTAGAAACAATCACAGATATTTTACTTGACAAGGGAGAGATCAAAGCTGAGGAAATTTGGGACATATACAAAAGGGCTCCTCGAATACCTCAGCCTGCAGTGAATGCAGTTGATGAATACGGAGCCCTAGTTTATGCGGGGCGTTGGGGAATACATGGGATTACACTCCCGGGAAGAGTTACATTTTCACCTGGAAATGTTGGGTTTTCAACCTTTGGCGCACCCAGGCCTATGGAGACCCAAAGAGTCAATGATGAAACATGGGAGCTGATTGATAACATCTGGGATAAAAGGGTTCAAGAAATTAAAGCTGAAGCTTCCGCTGAGGTTGAAGAAGACAAAGAAAGACCACAACTTTTGATGGCCAGCCATTTCTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009790 GO:0009791 GO:0009793 GO:0009941 GO:0010154 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0022414 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0032501 GO:0032502 GO:0034357 GO:0042623 GO:0042651 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0055035 GO:0061458 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA

Protein Analysis

838

Amino Acids

94.35

Weight (kDa)

5.98

Isoelectric Point (pI)

42.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RuvB_N	PF05496	297 - 403	1.6e-06	Holliday junction DNA helicase RuvB P-loop domain
AAA	PF00004	335 - 469	2.2e-41	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	499 - 538	3.9e-09	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009339)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64580
fragaria_vesca	FvH4_1g24180 FvH4_1g24180 FvH4_1g24180 FvH4_1g24180 FvH4_1g24180
malus_domestica	MD02G1315900.v1.1 MD07G1006000.v1.1
prunus_persica	Prupe.1G190200_v2.0.a1 Prupe.2G008800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0120571
rosa_laevigata	RLG00000018562
rosa_multiflora	Rmu_co8081184.1_g000001 Rmu_sc0001844.1_g000028 Rmu_sc0007380.1_g000017
rosa_roxburghii	Rroxscaffold_2G00123290
rosa_rugosa	Rorug02G0229500
rosa_samantha	Rh2AG285600 Rh2BG297200 Rh2CG274800 Rh2DG310900
rosa_wichuraiana	Rw2G022920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	636, 1397
AbsI	CCTCGAGG	1 cut(s)	1957
Acc36I	ACCTGC	1 cut(s)	661
AccB1I	GGYRCC	1 cut(s)	116
AccB7I	CCANNNNNTGG	1 cut(s)	442
AccBSI	CCGCTC	2 cut(s)	206, 515
AciI	CCGC	7 cut(s)	150, 204, 515, 700, 1508, 2273, 2457
AclWI	GGATC	9 cut(s)	365, 378, 472, 841, 1152, 1158, 1352, 1742, 1937
AcoI	YGGCCR	3 cut(s)	907, 1654, 2499
AcsI	RAATTY	7 cut(s)	141, 379, 523, 857, 1460, 1970, 2190
AcuI	CTGAAG	5 cut(s)	1256, 1481, 1494, 1967, 2469
AcvI	CACGTG	1 cut(s)	556
AcyI	GRCGYC	2 cut(s)	117, 550
AdeI	CACNNNGTG	1 cut(s)	2072
AfaI	GTAC	5 cut(s)	651, 945, 1005, 1669, 2126
AfiI	CCNNNNNNNGG	4 cut(s)	442, 1028, 1235, 2118
AjiI	CACGTC	1 cut(s)	1604
AjnI	CCWGG	7 cut(s)	803, 909, 1021, 1577, 1728, 2326, 2360
Alw21I	GWGCWC	1 cut(s)	1882
Alw26I	GTCTC	6 cut(s)	232, 406, 742, 1481, 1763, 2366
AlwI	GGATC	9 cut(s)	365, 378, 472, 841, 1152, 1158, 1352, 1742, 1937
AlwNI	CAGNNNCTG	3 cut(s)	653, 1279, 1321
Ama87I	CYCGRG	2 cut(s)	1957, 2305
AoxI	GGCC	8 cut(s)	327, 852, 907, 1069, 1264, 1654, 2363, 2499
ApoI	RAATTY	7 cut(s)	141, 379, 523, 857, 1460, 1970, 2190
AseI	ATTAAT	2 cut(s)	393, 1788
Asp700I	GAANNNNTTC	2 cut(s)	969, 2067
AspLEI	GCGC	5 cut(s)	119, 822, 1250, 1809, 2357
AspS9I	GGNCC	5 cut(s)	327, 693, 852, 1016, 1226
AsuC2I	CCSGG	3 cut(s)	851, 2306, 2307
AsuHPI	GGTGA	2 cut(s)	1073, 2316
AvaI	CYCGRG	2 cut(s)	1957, 2305
AvaII	GGWCC	3 cut(s)	693, 1016, 1226
BaeGI	GKGCMC	2 cut(s)	829, 1643
BalI	TGGCCA	2 cut(s)	909, 2501
BamHI	GGATCC	1 cut(s)	370
BanI	GGYRCC	1 cut(s)	116
BanII	GRGCYC	3 cut(s)	41, 2215, 2263
BauI	CACGAG	1 cut(s)	1134
BbrPI	CACGTG	1 cut(s)	556
BbsI	GAAGAC	3 cut(s)	1668, 1825, 2478
Bbv12I	GWGCWC	1 cut(s)	1882
BbvCI	CCTCAGC	3 cut(s)	2184, 2226, 2460
BccI	CCATC	6 cut(s)	512, 1282, 1286, 1415, 1420, 2491
BceAI	ACGGC	2 cut(s)	130, 807
BciT130I	CCWGG	7 cut(s)	805, 911, 1023, 1579, 1730, 2328, 2362
BciVI	GTATCC	1 cut(s)	637
BcnI	CCSGG	3 cut(s)	851, 2306, 2307
BcoDI	GTCTC	6 cut(s)	232, 406, 742, 1481, 1763, 2366
BfaI	CTAG	7 cut(s)	792, 1155, 1346, 1355, 1616, 1884, 2264
BfmI	CTRYAG	7 cut(s)	654, 681, 867, 1541, 2017, 2136, 2232
BfoI	RGCGCY	2 cut(s)	120, 823
BfuAI	ACCTGC	1 cut(s)	661
BfuI	GTATCC	1 cut(s)	637
BlpI	GCTNAGC	2 cut(s)	207, 2055
BmcAI	AGTACT	1 cut(s)	1669
Bme18I	GGWCC	3 cut(s)	693, 1016, 1226
BmeT110I	CYCGRG	2 cut(s)	1957, 2305
BmgBI	CACGTC	1 cut(s)	1604
BmgT120I	GGNCC	5 cut(s)	327, 693, 852, 1016, 1226
BmiI	GGNNCC	4 cut(s)	118, 372, 2214, 2260
BmrI	ACTGGG	1 cut(s)	891
BmsI	GCATC	5 cut(s)	220, 616, 993, 1139, 1192
BmuI	ACTGGG	1 cut(s)	891
BpiI	GAAGAC	3 cut(s)	1668, 1825, 2478
BpmI	CTGGAG	1 cut(s)	1551
Bpu10I	CCTNAGC	3 cut(s)	2184, 2226, 2460
Bpu1102I	GCTNAGC	2 cut(s)	207, 2055
BpuEI	CTTGAG	4 cut(s)	55, 287, 800, 1822
BpuMI	CCSGG	3 cut(s)	851, 2306, 2307
BsaAI	YACGTR	1 cut(s)	556
BsaBI	GATNNNNATC	1 cut(s)	1752
BsaHI	GRCGYC	2 cut(s)	117, 550
BsaI	GGTCTC	2 cut(s)	742, 2366
BsaJI	CCNNGG	5 cut(s)	85, 829, 1012, 2305, 2360
BsaWI	WCCGGW	1 cut(s)	1228
BsaXI	ACNNNNNCTCC	4 cut(s)	1889, 1919, 2250, 2280
Bsc4I	CCNNNNNNNGG	4 cut(s)	442, 1028, 1235, 2118
Bse118I	RCCGGY	1 cut(s)	1652
Bse1I	ACTGG	5 cut(s)	18, 436, 886, 1033, 1534
Bse3DI	GCAATG	1 cut(s)	1680
Bse8I	GATNNNNATC	1 cut(s)	1752
BseBI	CCWGG	7 cut(s)	805, 911, 1023, 1579, 1730, 2328, 2362
BseDI	CCNNGG	5 cut(s)	85, 829, 1012, 2305, 2360
BseGI	GGATG	6 cut(s)	67, 235, 523, 1297, 1426, 2108
BseJI	GATNNNNATC	1 cut(s)	1752
BseLI	CCNNNNNNNGG	4 cut(s)	442, 1028, 1235, 2118
BseMI	GCAATG	1 cut(s)	1680
BseMII	CTCAG	8 cut(s)	142, 151, 177, 221, 1685, 2175, 2240, 2451
BseNI	ACTGG	5 cut(s)	18, 436, 886, 1033, 1534
BseRI	GAGGAG	5 cut(s)	525, 1493, 1910, 1913, 2205
BseSI	GKGCMC	2 cut(s)	829, 1643
BsgI	GTGCAG	1 cut(s)	1986
BshFI	GGCC	8 cut(s)	329, 854, 909, 1071, 1266, 1656, 2365, 2501
BshNI	GGYRCC	1 cut(s)	116
BsiHKAI	GWGCWC	1 cut(s)	1882
BsiHKCI	CYCGRG	2 cut(s)	1957, 2305
BsiSI	CCGG	4 cut(s)	850, 1229, 1653, 2306
BslFI	GGGAC	2 cut(s)	373, 2210
BslI	CCNNNNNNNGG	4 cut(s)	442, 1028, 1235, 2118
BsmAI	GTCTC	6 cut(s)	232, 406, 742, 1481, 1763, 2366
BsmFI	GGGAC	2 cut(s)	373, 2210
BsmI	GAATGC	2 cut(s)	573, 2245
BsnI	GGCC	8 cut(s)	329, 854, 909, 1071, 1266, 1656, 2365, 2501
Bso31I	GGTCTC	2 cut(s)	742, 2366
BsoBI	CYCGRG	2 cut(s)	1957, 2305
Bsp1286I	GDGCHC	6 cut(s)	41, 829, 1643, 1882, 2215, 2263
Bsp1720I	GCTNAGC	2 cut(s)	207, 2055
Bsp19I	CCATGG	2 cut(s)	85, 1012
BspACI	CCGC	7 cut(s)	150, 204, 515, 700, 1508, 2273, 2457
BspANI	GGCC	8 cut(s)	329, 854, 909, 1071, 1266, 1656, 2365, 2501
BspCNI	CTCAG	8 cut(s)	141, 150, 176, 220, 1686, 2176, 2239, 2452
BspLI	GGNNCC	4 cut(s)	118, 372, 2214, 2260
BspMAI	CTGCAG	5 cut(s)	658, 685, 871, 1545, 2236
BspMI	ACCTGC	1 cut(s)	661
BspPI	GGATC	9 cut(s)	365, 378, 472, 841, 1152, 1158, 1352, 1742, 1937
BspQI	GCTCTTC	1 cut(s)	1602
BspT107I	GGYRCC	1 cut(s)	116
BspTNI	GGTCTC	2 cut(s)	742, 2366
BsrBI	CCGCTC	2 cut(s)	206, 515
BsrDI	GCAATG	1 cut(s)	1680
BsrFI	RCCGGY	1 cut(s)	1652
BsrI	ACTGG	5 cut(s)	18, 436, 886, 1033, 1534
BssAI	RCCGGY	1 cut(s)	1652
BssECI	CCNNGG	5 cut(s)	85, 829, 1012, 2305, 2360
BssNI	GRCGYC	2 cut(s)	117, 550
BssSI	CACGAG	1 cut(s)	1134
BssT1I	CCWWGG	3 cut(s)	85, 829, 1012
Bst2BI	CACGAG	1 cut(s)	1134
Bst2UI	CCWGG	7 cut(s)	805, 911, 1023, 1579, 1730, 2328, 2362
Bst4CI	ACNGT	4 cut(s)	288, 649, 673, 1667
Bst6I	CTCTTC	5 cut(s)	960, 1470, 1497, 1602, 2305
BstACI	GRCGYC	2 cut(s)	117, 550
BstAPI	GCANNNNNTGC	4 cut(s)	604, 990, 1721, 2240
BstBAI	YACGTR	1 cut(s)	556
BstDSI	CCRYGG	2 cut(s)	85, 1012
BstF5I	GGATG	6 cut(s)	67, 235, 523, 1297, 1426, 2108
BstH2I	RGCGCY	2 cut(s)	120, 823
BstHHI	GCGC	5 cut(s)	119, 822, 1250, 1809, 2357
BstMAI	GTCTC	6 cut(s)	232, 406, 742, 1481, 1763, 2366
BstMWI	GCNNNNNNNGC	9 cut(s)	176, 203, 604, 990, 1064, 1721, 1730, 1804, 2240
BstNI	CCWGG	7 cut(s)	805, 911, 1023, 1579, 1730, 2328, 2362
BstNSI	RCATGY	3 cut(s)	1451, 1737, 1878
BstSFI	CTRYAG	7 cut(s)	654, 681, 867, 1541, 2017, 2136, 2232
BstSLI	GKGCMC	2 cut(s)	829, 1643
BstV2I	GAAGAC	3 cut(s)	1668, 1825, 2478
BstX2I	RGATCY	6 cut(s)	370, 1144, 1163, 1357, 1747, 1929
BstXI	CCANNNNNNTGG	1 cut(s)	2368
BstYI	RGATCY	6 cut(s)	370, 1144, 1163, 1357, 1747, 1929
BsuI	GTATCC	1 cut(s)	637
BsuRI	GGCC	8 cut(s)	329, 854, 909, 1071, 1266, 1656, 2365, 2501
BtgI	CCRYGG	2 cut(s)	85, 1012
BtrI	CACGTC	1 cut(s)	1604
BtsCI	GGATG	6 cut(s)	67, 235, 523, 1297, 1426, 2108
BtsI	GCAGTG	2 cut(s)	1037, 2241
BtsIMutI	CAGTG	5 cut(s)	480, 1037, 1095, 1812, 2241
BveI	ACCTGC	1 cut(s)	661
CaiI	CAGNNNCTG	3 cut(s)	653, 1279, 1321
CfoI	GCGC	5 cut(s)	119, 822, 1250, 1809, 2357
Cfr10I	RCCGGY	1 cut(s)	1652
Cfr13I	GGNCC	5 cut(s)	327, 693, 852, 1016, 1226
Cfr9I	CCCGGG	1 cut(s)	2305
CseI	GACGC	1 cut(s)	539
Csp6I	GTAC	5 cut(s)	650, 944, 1004, 1668, 2125
CspCI	CAANNNNNGTGG	6 cut(s)	15, 50, 1204, 1239, 2475, 2510
CviQI	GTAC	5 cut(s)	650, 944, 1004, 1668, 2125
DinI	GGCGCC	1 cut(s)	118
DraI	TTTAAA	1 cut(s)	954
DraIII	CACNNNGTG	1 cut(s)	2072
EaeI	YGGCCR	3 cut(s)	907, 1654, 2499
Eam1104I	CTCTTC	5 cut(s)	960, 1470, 1497, 1602, 2305
EarI	CTCTTC	5 cut(s)	960, 1470, 1497, 1602, 2305
Eco130I	CCWWGG	3 cut(s)	85, 829, 1012
Eco147I	AGGCCT	3 cut(s)	1071, 1266, 2365
Eco24I	GRGCYC	3 cut(s)	41, 2215, 2263
Eco31I	GGTCTC	2 cut(s)	742, 2366
Eco32I	GATATC	2 cut(s)	1351, 1777
Eco47I	GGWCC	3 cut(s)	693, 1016, 1226
Eco57I	CTGAAG	5 cut(s)	1256, 1481, 1494, 1967, 2469
Eco72I	CACGTG	1 cut(s)	556
Eco88I	CYCGRG	2 cut(s)	1957, 2305
EcoO109I	RGGNCCY	1 cut(s)	327
EcoRI	GAATTC	2 cut(s)	141, 523
EcoRII	CCWGG	7 cut(s)	803, 909, 1021, 1577, 1728, 2326, 2360
EcoRV	GATATC	2 cut(s)	1351, 1777
EcoT14I	CCWWGG	3 cut(s)	85, 829, 1012
EcoT38I	GRGCYC	3 cut(s)	41, 2215, 2263
EgeI	GGCGCC	1 cut(s)	118
EheI	GGCGCC	1 cut(s)	118
ErhI	CCWWGG	3 cut(s)	85, 829, 1012
FalI	AAGNNNNNCTT	4 cut(s)	990, 1022, 1254, 1286
FaqI	GGGAC	2 cut(s)	373, 2210
FauI	CCCGC	1 cut(s)	2266
FauNDI	CATATG	1 cut(s)	689
FokI	GGATG	6 cut(s)	54, 242, 530, 1304, 1433, 2095
FriOI	GRGCYC	3 cut(s)	41, 2215, 2263
FspBI	CTAG	7 cut(s)	792, 1155, 1346, 1355, 1616, 1884, 2264
GlaI	GCGC	5 cut(s)	118, 821, 1249, 1808, 2356
GsuI	CTGGAG	1 cut(s)	1551
HaeII	RGCGCY	2 cut(s)	120, 823
HaeIII	GGCC	8 cut(s)	329, 854, 909, 1071, 1266, 1656, 2365, 2501
HapII	CCGG	4 cut(s)	850, 1229, 1653, 2306
HgaI	GACGC	1 cut(s)	539
HhaI	GCGC	5 cut(s)	119, 822, 1250, 1809, 2357
Hin1I	GRCGYC	2 cut(s)	117, 550
Hin6I	GCGC	5 cut(s)	117, 820, 1248, 1807, 2355
HinP1I	GCGC	5 cut(s)	117, 820, 1248, 1807, 2355
HincII	GTYRAC	3 cut(s)	562, 1915, 2098
HindII	GTYRAC	3 cut(s)	562, 1915, 2098
HindIII	AAGCTT	2 cut(s)	2057, 2451
HinfI	GANTC	6 cut(s)	200, 875, 1296, 1489, 1978, 2382
HpaII	CCGG	4 cut(s)	850, 1229, 1653, 2306
HphI	GGTGA	2 cut(s)	1073, 2316
Hpy166II	GTNNAC	6 cut(s)	562, 1004, 1226, 1858, 1915, 2098
Hpy188I	TCNGA	8 cut(s)	101, 140, 778, 1275, 1474, 1849, 1977, 1986
Hpy188III	TCNNGA	7 cut(s)	374, 974, 1499, 1745, 1839, 2072, 2435
Hpy8I	GTNNAC	6 cut(s)	562, 1004, 1226, 1858, 1915, 2098
Hpy99I	CGWCG	1 cut(s)	191
HpyCH4III	ACNGT	4 cut(s)	288, 649, 673, 1667
HpyCH4IV	ACGT	3 cut(s)	555, 1219, 1603
HpyF10VI	GCNNNNNNNGC	9 cut(s)	176, 203, 604, 990, 1064, 1721, 1730, 1804, 2240
HpySE526I	ACGT	3 cut(s)	555, 1219, 1603
Hsp92I	GRCGYC	2 cut(s)	117, 550
HspAI	GCGC	5 cut(s)	117, 820, 1248, 1807, 2355
KasI	GGCGCC	1 cut(s)	116
LguI	GCTCTTC	1 cut(s)	1602
LmnI	GCTCC	9 cut(s)	152, 321, 512, 928, 1532, 1885, 2218, 2258, 2401
LweI	GCATC	5 cut(s)	220, 616, 993, 1139, 1192
MaeI	CTAG	7 cut(s)	792, 1155, 1346, 1355, 1616, 1884, 2264
MaeII	ACGT	3 cut(s)	555, 1219, 1603
MaeIII	GTNAC	3 cut(s)	401, 551, 2314
MbiI	CCGCTC	2 cut(s)	206, 515
MflI	RGATCY	6 cut(s)	370, 1144, 1163, 1357, 1747, 1929
MhlI	GDGCHC	6 cut(s)	41, 829, 1643, 1882, 2215, 2263
MlsI	TGGCCA	2 cut(s)	909, 2501
MluNI	TGGCCA	2 cut(s)	909, 2501
Mly113I	GGCGCC	1 cut(s)	117
MlyI	GAGTC	3 cut(s)	209, 1483, 2391
MmeI	TCCRAC	3 cut(s)	183, 563, 1907
Mox20I	TGGCCA	2 cut(s)	909, 2501
MroXI	GAANNNNTTC	2 cut(s)	969, 2067
MscI	TGGCCA	2 cut(s)	909, 2501
MseI	TTAA	9 cut(s)	20, 353, 393, 420, 953, 1688, 1788, 2117, 2442
Msp20I	TGGCCA	2 cut(s)	909, 2501
MspA1I	CMGCKG	4 cut(s)	152, 1718, 2135, 2459
MspI	CCGG	4 cut(s)	850, 1229, 1653, 2306
Mva1269I	GAATGC	2 cut(s)	573, 2245
MvaI	CCWGG	7 cut(s)	805, 911, 1023, 1579, 1730, 2328, 2362
MwoI	GCNNNNNNNGC	9 cut(s)	176, 203, 604, 990, 1064, 1721, 1730, 1804, 2240
NarI	GGCGCC	1 cut(s)	117
NciI	CCSGG	3 cut(s)	851, 2306, 2307
NcoI	CCATGG	2 cut(s)	85, 1012
NdeI	CATATG	1 cut(s)	689
NlaIV	GGNNCC	4 cut(s)	118, 372, 2214, 2260
NmeAIII	GCCGAG	1 cut(s)	223
NmuCI	GTSAC	2 cut(s)	401, 551
NspI	RCATGY	3 cut(s)	1451, 1737, 1878
PaeI	GCATGC	2 cut(s)	1451, 1737
PaeR7I	CTCGAG	1 cut(s)	1957
PceI	AGGCCT	3 cut(s)	1071, 1266, 2365
PciSI	GCTCTTC	1 cut(s)	1602
PcsI	WCGNNNNNNNCGW	2 cut(s)	183, 195
PctI	GAATGC	2 cut(s)	573, 2245
PdmI	GAANNNNTTC	2 cut(s)	969, 2067
PfeI	GAWTC	3 cut(s)	875, 1296, 1978
PflMI	CCANNNNNTGG	1 cut(s)	442
PfoI	TCCNGGA	1 cut(s)	1021
PleI	GAGTC	3 cut(s)	208, 1483, 2390
PluTI	GGCGCC	1 cut(s)	120
PmaCI	CACGTG	1 cut(s)	556
PmlI	CACGTG	1 cut(s)	556
PpsI	GAGTC	3 cut(s)	208, 1483, 2390
Ppu21I	YACGTR	1 cut(s)	556
PshBI	ATTAAT	2 cut(s)	393, 1788
PsiI	TTATAA	2 cut(s)	636, 1397
Psp6I	CCWGG	7 cut(s)	803, 909, 1021, 1577, 1728, 2326, 2360
PspCI	CACGTG	1 cut(s)	556
PspGI	CCWGG	7 cut(s)	803, 909, 1021, 1577, 1728, 2326, 2360
PspN4I	GGNNCC	4 cut(s)	118, 372, 2214, 2260
PspPI	GGNCC	5 cut(s)	327, 693, 852, 1016, 1226
PspXI	VCTCGAGB	1 cut(s)	1957
PstI	CTGCAG	5 cut(s)	658, 685, 871, 1545, 2236
PstNI	CAGNNNCTG	3 cut(s)	653, 1279, 1321
PsuI	RGATCY	6 cut(s)	370, 1144, 1163, 1357, 1747, 1929
PvuII	CAGCTG	2 cut(s)	1718, 2135
RsaI	GTAC	5 cut(s)	651, 945, 1005, 1669, 2126
RsaNI	GTAC	5 cut(s)	650, 944, 1004, 1668, 2125
SapI	GCTCTTC	1 cut(s)	1602
SaqAI	TTAA	9 cut(s)	20, 353, 393, 420, 953, 1688, 1788, 2117, 2442
Sau96I	GGNCC	5 cut(s)	327, 693, 852, 1016, 1226
ScaI	AGTACT	1 cut(s)	1669
SchI	GAGTC	3 cut(s)	209, 1483, 2391
SduI	GDGCHC	6 cut(s)	41, 829, 1643, 1882, 2215, 2263
SfaNI	GCATC	5 cut(s)	220, 616, 993, 1139, 1192
SfcI	CTRYAG	7 cut(s)	654, 681, 867, 1541, 2017, 2136, 2232
SfoI	GGCGCC	1 cut(s)	118
Sfr274I	CTCGAG	1 cut(s)	1957
SinI	GGWCC	3 cut(s)	693, 1016, 1226
SlaI	CTCGAG	1 cut(s)	1957
SmaI	CCCGGG	1 cut(s)	2307
SmlI	CTYRAG	5 cut(s)	34, 266, 815, 1837, 1957
SmoI	CTYRAG	5 cut(s)	34, 266, 815, 1837, 1957
SphI	GCATGC	2 cut(s)	1451, 1737
SseBI	AGGCCT	3 cut(s)	1071, 1266, 2365
SsiI	CCGC	7 cut(s)	150, 204, 515, 700, 1508, 2273, 2457
SspDI	GGCGCC	1 cut(s)	116
SspMI	CTAG	7 cut(s)	792, 1155, 1346, 1355, 1616, 1884, 2264
StuI	AGGCCT	3 cut(s)	1071, 1266, 2365
StyI	CCWWGG	3 cut(s)	85, 829, 1012
TaaI	ACNGT	4 cut(s)	288, 649, 673, 1667
TaiI	ACGT	3 cut(s)	558, 1222, 1606
TaqI	TCGA	2 cut(s)	1958, 2218
TaqII	GACCGA	2 cut(s)	579, 766
TatI	WGTACW	2 cut(s)	1003, 1667
TauI	GCSGC	1 cut(s)	703
TfiI	GAWTC	3 cut(s)	875, 1296, 1978
Tru1I	TTAA	9 cut(s)	20, 353, 393, 420, 953, 1688, 1788, 2117, 2442
Tru9I	TTAA	9 cut(s)	20, 353, 393, 420, 953, 1688, 1788, 2117, 2442
TscAI	CASTG	5 cut(s)	480, 1044, 1102, 1819, 2241
TseFI	GTSAC	2 cut(s)	401, 551
Tsp45I	GTSAC	2 cut(s)	401, 551
TspDTI	ATGAA	8 cut(s)	17, 303, 978, 1753, 2091, 2097, 2265, 2406
TspGWI	ACGGA	4 cut(s)	961, 1457, 2101, 2271
TspMI	CCCGGG	1 cut(s)	2305
TspRI	CASTG	5 cut(s)	480, 1044, 1102, 1819, 2241
Van91I	CCANNNNNTGG	1 cut(s)	442
VpaK11BI	GGWCC	3 cut(s)	693, 1016, 1226
VspI	ATTAAT	2 cut(s)	393, 1788
XapI	RAATTY	7 cut(s)	141, 379, 523, 857, 1460, 1970, 2190
XceI	RCATGY	3 cut(s)	1451, 1737, 1878
XcmI	CCANNNNNNNNNTGG	2 cut(s)	579, 1019
XhoI	CTCGAG	1 cut(s)	1957
XmaI	CCCGGG	1 cut(s)	2305
XmnI	GAANNNNTTC	2 cut(s)	969, 2067
XspI	CTAG	7 cut(s)	792, 1155, 1346, 1355, 1616, 1884, 2264
ZrmI	AGTACT	1 cut(s)	1669

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.