AT5G65165

Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013888
Physical Location & Seq
Reverse (-)
26034422 .. 26036057
1636 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G65165.2

Sequence Viewer

Length: 930 bp
ATGTCGTCTGTCTTGCGGTTGTTGGGGCGGAGAATCTGTAATCCAGCGGCGGAGAAAGTGCGTTTGAGCAGTAGCCTAAGCGGTGGTGGAGATTTTCCAATCTTAAATGGACATAAAGCGGCTCAAGACTTATCCAAAGATACTCTAAAATCCCAAGACATAACGAAAGAGAAAGAAGGCCAACACAAAGAAGTTAAGAAGGAGTTCAAGATTTATAGATGGAATCCTGACAAGCCTAACTCTAAACCATTTCTTCAATCTTTCTTCGTTGATCTTTCCTCTTGTGGTCCCATGGTTTTGGACGTGCTGCAGAAGATAAAAGCAGAGGATGATGCCAGTTTGAGTTACAGAAGATCATGTAGAGAAGGGATATGTGGATCTTGTTCAATGAACATTGACGGTACTAACACTGTCGCATGTCTTAAACCCATAAACCCAAACACGTCAAAGCCCACCATAATTACTCCATTGCCTCACATGTACGTCATCAAAGACCTAGTCGTCGACCTCACTAATTTTTACCAACAATACAAATCAATGGAGCCATGGTTAAAGACGAGGAAGCCTCCAAAAGACGGACGCGAACACCGGCAATCACCAAAAGACAGGAAGAAACTAGACGGGCTATACGAATGCATATTGTGCGCGTGTTGTACCACATCATGTCCTTCTTATTGGTGGAACCCCGAGGAGTTTCCAGGACCTGCGGCTTTACTACAAGCGTACCGTTGGATCAGCGATAGCCGCGATGAATACAGAGAAGAGAGGCTACAGGCAATAACTGAGAGTGAAACCAAAGTATATAGATGCAGAGCAATCAAGAACTGTACGGCCACTTGTCCTAAAGGACTTAACCCAGCTAGTGCCATTTTGAAAATGAAGTCTAAGCATTTGCTCTCCGATCCGTTAGTGAGAACAGAGAGTGTCTAG

Protein Analysis

309

Amino Acids

34.98

Weight (kDa)

9.08

Isoelectric Point (pI)

55.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2_3 PF13085 69 - 174 7.6e-33 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013888)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65165 AT5G65165
fragaria_vesca FvH4_1g21120
prunus_persica Prupe.6G182900_v2.0.a1 Prupe.6G183100_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0114901
rosa_laevigata RLG00000018133
rosa_multiflora Rmu_sc0002764.1_g000022
rosa_roxburghii Rroxscaffold_2G00130430
rosa_rugosa Rorug02G0191500 Rorug02G0191600 Rorug02G0191600
rosa_samantha Rh2AG250200 Rh2BG259800 Rh2CG254600 Rh2DG258300
rosa_wichuraiana Rw2G019510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 500
Acc36I ACCTGC 1 cut(s) 712
AccI GTMKAC 1 cut(s) 504
AccII CGCG 3 cut(s) 582, 647, 747
AciI CCGC 8 cut(s) 16, 28, 47, 50, 81, 119, 707, 745
AclWI GGATC 3 cut(s) 385, 740, 896
AcoI YGGCCR 1 cut(s) 831
AfaI GTAC 5 cut(s) 403, 482, 655, 725, 829
AfiI CCNNNNNNNGG 1 cut(s) 575
AflIII ACRYGT 2 cut(s) 441, 477
AgsI TTSAA 4 cut(s) 208, 257, 387, 874
AjiI CACGTC 2 cut(s) 304, 444
AjnI CCWGG 1 cut(s) 697
AluBI AGCT 1 cut(s) 860
AluI AGCT 1 cut(s) 860
AlwI GGATC 3 cut(s) 385, 740, 896
AlwNI CAGNNNCTG 1 cut(s) 704
Ama87I CYCGRG 1 cut(s) 686
AoxI GGCC 2 cut(s) 178, 831
ApeKI GCWGC 1 cut(s) 307
ArsI GACNNNNNNTTYG 2 cut(s) 483, 515
Asp700I GAANNNNTTC 1 cut(s) 203
AspLEI GCGC 1 cut(s) 647
AspS9I GGNCC 2 cut(s) 287, 701
AsuHPI GGTGA 1 cut(s) 588
AvaI CYCGRG 1 cut(s) 686
AvaII GGWCC 2 cut(s) 287, 701
BaeI ACNNNNGTAYC 2 cut(s) 707, 740
BarI GAAGNNNNNNTAC 2 cut(s) 753, 785
BbvI GCAGC 1 cut(s) 294
BccI CCATC 1 cut(s) 213
BceAI ACGGC 1 cut(s) 846
BciT130I CCWGG 1 cut(s) 699
BfaI CTAG 4 cut(s) 497, 617, 861, 928
BfmI CTRYAG 2 cut(s) 308, 770
BfuAI ACCTGC 1 cut(s) 712
BisI GCNGC 5 cut(s) 48, 120, 308, 708, 745
BlsI GCNGC 5 cut(s) 49, 121, 309, 709, 746
Bme1390I CCNGG 1 cut(s) 699
Bme18I GGWCC 2 cut(s) 287, 701
BmeT110I CYCGRG 1 cut(s) 686
BmgBI CACGTC 2 cut(s) 304, 444
BmgT120I GGNCC 2 cut(s) 287, 701
BmiI GGNNCC 3 cut(s) 289, 543, 683
BmrFI CCNGG 1 cut(s) 699
BmsI GCATC 2 cut(s) 322, 797
BplI GAGNNNNNCTC 2 cut(s) 550, 582
Bpu10I CCTNAGC 1 cut(s) 77
BpuEI CTTGAG 1 cut(s) 108
BsaJI CCNNGG 3 cut(s) 291, 545, 687
BsaXI ACNNNNNCTCC 4 cut(s) 22, 52, 533, 563
Bsc4I CCNNNNNNNGG 1 cut(s) 575
Bse118I RCCGGY 1 cut(s) 588
Bse1I ACTGG 1 cut(s) 336
Bse3DI GCAATG 1 cut(s) 467
BseBI CCWGG 1 cut(s) 699
BseDI CCNNGG 3 cut(s) 291, 545, 687
BseGI GGATG 1 cut(s) 334
BseLI CCNNNNNNNGG 1 cut(s) 575
BseMI GCAATG 1 cut(s) 467
BseMII CTCAG 1 cut(s) 774
BseNI ACTGG 1 cut(s) 336
BseRI GAGGAG 1 cut(s) 704
BseXI GCAGC 1 cut(s) 294
BseYI CCCAGC 1 cut(s) 856
Bsh1236I CGCG 3 cut(s) 582, 647, 747
BshFI GGCC 2 cut(s) 180, 833
BsiHKCI CYCGRG 1 cut(s) 686
BsiSI CCGG 1 cut(s) 589
BslFI GGGAC 1 cut(s) 273
BslI CCNNNNNNNGG 1 cut(s) 575
BsmFI GGGAC 1 cut(s) 273
BsmI GAATGC 1 cut(s) 638
BsnI GGCC 2 cut(s) 180, 833
BsoBI CYCGRG 1 cut(s) 686
Bsp143I GATC 5 cut(s) 271, 353, 377, 732, 901
Bsp19I CCATGG 2 cut(s) 291, 545
BspACI CCGC 8 cut(s) 16, 28, 47, 50, 81, 119, 707, 745
BspANI GGCC 2 cut(s) 180, 833
BspCNI CTCAG 1 cut(s) 775
BspFNI CGCG 3 cut(s) 582, 647, 747
BspLI GGNNCC 3 cut(s) 289, 543, 683
BspMAI CTGCAG 1 cut(s) 312
BspMI ACCTGC 1 cut(s) 712
BspPI GGATC 3 cut(s) 385, 740, 896
BsrDI GCAATG 1 cut(s) 467
BsrFI RCCGGY 1 cut(s) 588
BsrI ACTGG 1 cut(s) 336
BssAI RCCGGY 1 cut(s) 588
BssECI CCNNGG 3 cut(s) 291, 545, 687
BssMI GATC 5 cut(s) 271, 353, 377, 732, 901
BssT1I CCWWGG 2 cut(s) 291, 545
Bst2UI CCWGG 1 cut(s) 699
Bst4CI ACNGT 4 cut(s) 401, 412, 728, 827
Bst6I CTCTTC 1 cut(s) 756
BstAPI GCANNNNNTGC 1 cut(s) 642
BstDEI CTNAG 3 cut(s) 77, 783, 885
BstDSI CCRYGG 2 cut(s) 291, 545
BstF5I GGATG 1 cut(s) 334
BstFNI CGCG 3 cut(s) 582, 647, 747
BstHHI GCGC 1 cut(s) 647
BstKTI GATC 5 cut(s) 274, 356, 380, 735, 904
BstMBI GATC 5 cut(s) 271, 353, 377, 732, 901
BstMWI GCNNNNNNNGC 2 cut(s) 642, 744
BstNI CCWGG 1 cut(s) 699
BstNSI RCATGY 2 cut(s) 420, 481
BstSCI CCNGG 1 cut(s) 697
BstSFI CTRYAG 2 cut(s) 308, 770
BstUI CGCG 3 cut(s) 582, 647, 747
BstV1I GCAGC 1 cut(s) 294
BstX2I RGATCY 1 cut(s) 377
BstXI CCANNNNNNTGG 1 cut(s) 298
BstYI RGATCY 1 cut(s) 377
BsuRI GGCC 2 cut(s) 180, 833
BtgI CCRYGG 2 cut(s) 291, 545
BtgZI GCGATG 1 cut(s) 762
BtrI CACGTC 2 cut(s) 304, 444
BtsCI GGATG 1 cut(s) 334
BtsIMutI CAGTG 1 cut(s) 408
BveI ACCTGC 1 cut(s) 712
CaiI CAGNNNCTG 1 cut(s) 704
CfoI GCGC 1 cut(s) 647
Cfr10I RCCGGY 1 cut(s) 588
Cfr13I GGNCC 2 cut(s) 287, 701
CseI GACGC 1 cut(s) 588
Csp6I GTAC 5 cut(s) 402, 481, 654, 724, 828
CviAII CATG 6 cut(s) 292, 357, 417, 478, 546, 663
CviQI GTAC 5 cut(s) 402, 481, 654, 724, 828
DdeI CTNAG 3 cut(s) 77, 783, 885
DpnI GATC 5 cut(s) 273, 355, 379, 734, 903
DpnII GATC 5 cut(s) 271, 353, 377, 732, 901
DrdI GACNNNNNNGTC 1 cut(s) 500
DseDI GACNNNNNNGTC 1 cut(s) 500
EaeI YGGCCR 1 cut(s) 831
Eam1104I CTCTTC 1 cut(s) 756
EarI CTCTTC 1 cut(s) 756
EciI GGCGGA 2 cut(s) 43, 65
Eco130I CCWWGG 2 cut(s) 291, 545
Eco47I GGWCC 2 cut(s) 287, 701
Eco88I CYCGRG 1 cut(s) 686
EcoO109I RGGNCCY 1 cut(s) 701
EcoRII CCWGG 1 cut(s) 697
EcoT14I CCWWGG 2 cut(s) 291, 545
EcoT22I ATGCAT 1 cut(s) 638
ErhI CCWWGG 2 cut(s) 291, 545
FaeI CATG 6 cut(s) 295, 360, 420, 481, 549, 666
FaqI GGGAC 1 cut(s) 273
FatI CATG 6 cut(s) 291, 356, 416, 477, 545, 662
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 5 cut(s) 48, 120, 308, 708, 745
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 5 cut(s) 48, 120, 308, 708, 745
FspBI CTAG 4 cut(s) 497, 617, 861, 928
GlaI GCGC 1 cut(s) 646
GluI GCNGC 5 cut(s) 48, 120, 308, 708, 745
GsaI CCCAGC 1 cut(s) 860
HaeIII GGCC 2 cut(s) 180, 833
HapII CCGG 1 cut(s) 589
HgaI GACGC 1 cut(s) 588
HhaI GCGC 1 cut(s) 647
Hin1II CATG 6 cut(s) 295, 360, 420, 481, 549, 666
Hin6I GCGC 1 cut(s) 645
HinP1I GCGC 1 cut(s) 645
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HinfI GANTC 2 cut(s) 33, 223
HpaII CCGG 1 cut(s) 589
HphI GGTGA 1 cut(s) 588
Hpy166II GTNNAC 1 cut(s) 505
Hpy188I TCNGA 1 cut(s) 901
Hpy188III TCNNGA 4 cut(s) 125, 208, 227, 820
Hpy8I GTNNAC 1 cut(s) 505
Hpy99I CGWCG 1 cut(s) 506
HpyAV CCTTC 4 cut(s) 170, 193, 359, 678
HpyCH4III ACNGT 4 cut(s) 401, 412, 728, 827
HpyCH4IV ACGT 3 cut(s) 303, 443, 483
HpyCH4V TGCA 3 cut(s) 310, 636, 810
HpyF10VI GCNNNNNNNGC 2 cut(s) 642, 744
HpyF3I CTNAG 3 cut(s) 77, 783, 885
HpySE526I ACGT 3 cut(s) 303, 443, 483
Hsp92II CATG 6 cut(s) 295, 360, 420, 481, 549, 666
HspAI GCGC 1 cut(s) 645
Kzo9I GATC 5 cut(s) 271, 353, 377, 732, 901
LmnI GCTCC 1 cut(s) 541
Lsp1109I GCAGC 1 cut(s) 294
LweI GCATC 2 cut(s) 322, 797
MaeI CTAG 4 cut(s) 497, 617, 861, 928
MaeII ACGT 3 cut(s) 303, 443, 483
MaeIII GTNAC 1 cut(s) 344
MalI GATC 5 cut(s) 273, 355, 379, 734, 903
MboI GATC 5 cut(s) 271, 353, 377, 732, 901
MboII GAAGA 6 cut(s) 245, 256, 325, 363, 622, 773
MflI RGATCY 1 cut(s) 377
MluCI AATT 2 cut(s) 459, 514
MmeI TCCRAC 1 cut(s) 710
MnlI CCTC 8 cut(s) 289, 319, 483, 518, 552, 576, 682, 759
Mph1103I ATGCAT 1 cut(s) 638
MroXI GAANNNNTTC 1 cut(s) 203
MseI TTAA 5 cut(s) 104, 195, 423, 551, 852
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 1 cut(s) 589
MspR9I CCNGG 1 cut(s) 699
Mva1269I GAATGC 1 cut(s) 638
MvaI CCWGG 1 cut(s) 699
MvnI CGCG 3 cut(s) 582, 647, 747
MwoI GCNNNNNNNGC 2 cut(s) 642, 744
NcoI CCATGG 2 cut(s) 291, 545
NdeII GATC 5 cut(s) 271, 353, 377, 732, 901
NlaIII CATG 6 cut(s) 295, 360, 420, 481, 549, 666
NlaIV GGNNCC 3 cut(s) 289, 543, 683
NsiI ATGCAT 1 cut(s) 638
NspI RCATGY 2 cut(s) 420, 481
PciI ACATGT 1 cut(s) 477
PcsI WCGNNNNNNNCGW 1 cut(s) 627
PctI GAATGC 1 cut(s) 638
PdmI GAANNNNTTC 1 cut(s) 203
PfeI GAWTC 2 cut(s) 33, 223
PflFI GACNNNGTC 1 cut(s) 497
PfoI TCCNGGA 1 cut(s) 697
PkrI GCNGC 5 cut(s) 49, 121, 309, 709, 746
PpuMI RGGWCCY 1 cut(s) 701
PscI ACATGT 1 cut(s) 477
Psp5II RGGWCCY 1 cut(s) 701
Psp6I CCWGG 1 cut(s) 697
PspFI CCCAGC 1 cut(s) 856
PspGI CCWGG 1 cut(s) 697
PspN4I GGNNCC 3 cut(s) 289, 543, 683
PspPI GGNCC 2 cut(s) 287, 701
PspPPI RGGWCCY 1 cut(s) 701
PstI CTGCAG 1 cut(s) 312
PstNI CAGNNNCTG 1 cut(s) 704
PsuI RGATCY 1 cut(s) 377
PsyI GACNNNGTC 1 cut(s) 497
RsaI GTAC 5 cut(s) 403, 482, 655, 725, 829
RsaNI GTAC 5 cut(s) 402, 481, 654, 724, 828
SalI GTCGAC 1 cut(s) 503
SaqAI TTAA 5 cut(s) 104, 195, 423, 551, 852
SatI GCNGC 5 cut(s) 48, 120, 308, 708, 745
Sau3AI GATC 5 cut(s) 271, 353, 377, 732, 901
Sau96I GGNCC 2 cut(s) 287, 701
ScrFI CCNGG 1 cut(s) 699
SetI ASST 7 cut(s) 306, 446, 486, 498, 510, 706, 862
SfaNI GCATC 2 cut(s) 322, 797
SfcI CTRYAG 2 cut(s) 308, 770
SinI GGWCC 2 cut(s) 287, 701
SmlI CTYRAG 1 cut(s) 123
SmoI CTYRAG 1 cut(s) 123
Sse9I AATT 2 cut(s) 459, 514
SsiI CCGC 8 cut(s) 16, 28, 47, 50, 81, 119, 707, 745
SspMI CTAG 4 cut(s) 497, 617, 861, 928
StyD4I CCNGG 1 cut(s) 697
StyI CCWWGG 2 cut(s) 291, 545
TaaI ACNGT 4 cut(s) 401, 412, 728, 827
TaiI ACGT 3 cut(s) 306, 446, 486
TaqI TCGA 1 cut(s) 504
TasI AATT 2 cut(s) 459, 514
TauI GCSGC 4 cut(s) 50, 122, 710, 747
TfiI GAWTC 2 cut(s) 33, 223
Tru1I TTAA 5 cut(s) 104, 195, 423, 551, 852
Tru9I TTAA 5 cut(s) 104, 195, 423, 551, 852
TscAI CASTG 1 cut(s) 415
TseI GCWGC 1 cut(s) 307
TspDTI ATGAA 3 cut(s) 404, 765, 893
TspGWI ACGGA 2 cut(s) 591, 894
TspRI CASTG 1 cut(s) 415
Tth111I GACNNNGTC 1 cut(s) 497
VpaK11BI GGWCC 2 cut(s) 287, 701
XceI RCATGY 2 cut(s) 420, 481
XmiI GTMKAC 1 cut(s) 504
XmnI GAANNNNTTC 1 cut(s) 203
XspI CTAG 4 cut(s) 497, 617, 861, 928
Zsp2I ATGCAT 1 cut(s) 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.