AT5G65170

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012018
Physical Location & Seq
Forward (+)
26041045 .. 26043267
2223 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G65170.2

Sequence Viewer

Length: 1089 bp
ATGGAATCCGGCAATAGTAGTAGCATGCAATCCTCAAGCGGCGGTGGTGGTGGAGGAGATCAAGAAGAGTACGACTCACGCGCCGATCAATCAATTTCTGCTTTATTTAACCACAACAACAACAACAACAACCCAACCACCGTCTCCCCCAACATCGCTGTTCCAACACAGCTTGACGCTCTCATAGCTAACTACTTCAACACTTCTTGGTCAGCGGATAATCCTCTATGGTCTACAACGGCTACGCAGCCCACCGATGGCTCTAGACCAGTACCACCGCCGATTTCGTCAGAACAAGTCTTCTTCACAAACCCACTTCAACAAAATCTGAGAACAGTTCCTAACACAAACACAACTAGTCCCATTTGTTCCGTCCCAACCGACAAGAAAAACGGTCTTGCGACAACACGGAATCCAAAGAAGAGATCTCGAGTCTCGAGACGAGCGCCTACGACTGTTTTGACCACCGACACATCCAACTTCAGAGCCATGGTTCAAGAATTCACGGGTAATCCTTCAACTCCTTTCACCGGATTATCTTCATCTTTTCCAAGATCACGATTTGATCTCTTCGGTTCTTCTTCTTCTTCATCCTCACGACCCATGAAACCCTTTCCTCACAAACTCATCTCTCCATCGACGTTGAATCATCATTACCTTCCTCCTTCTTCAGAGTACCATCATCATCATCAGCATCAGAATCTTCTCCTTAATATGAACACGCCAAATATCTCAAACCCTTTTCTCAACAACTCTTTAACGGATCAACCGAAACCCTCGAGCTTGAGAACGAGTAATGGTTTTGGACACGTGGACGTTGGAACAAACTTTGAGGGGCTTCACAACATTATCGTCTCTTCTTCATCGTCGATGACACAGCCAACCCTAAATACTATCCATGGACCAGAAAAGAGTACTGAAGCCGAAAATGATAATAATATGCTAAGATCCATCAATGGTAATGATCAACCCATGGTCCAAAGATCCGATGGTTACACACAACCTGGTGCTTCTGGATCCGAGAATAATCTATCGGCCGTGAGAAGTGAAGGTATGGTGGAATTATCTTGGATCTCTTCTTCTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

362

Amino Acids

39.23

Weight (kDa)

6.3

Isoelectric Point (pI)

61.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 148 - 169 1.5e-08 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012018)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65170 AT5G65170
fragaria_vesca FvH4_1g21110
malus_domestica MD01G1027700.v1.1 MD15G1325900.v1.1
prunus_persica Prupe.6G183200_v2.0.a1 Prupe.6G183200_v2.0.a1
pyrus_communis pycom01g05580 pycom15g28700
rosa_chinensis RchiOBHm_Chr2g0114891
rosa_laevigata RLG00000018131
rosa_multiflora Rmu_sc0002764.1_g000028
rosa_roxburghii Rroxscaffold_2G00130390
rosa_rugosa Rorug02G0191400
rosa_samantha Rh2AG250000 Rh2BG259600 Rh2CG254400 Rh2DG258200
rosa_wichuraiana Rw2G019500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 233
AccII CGCG 1 cut(s) 81
AciI CCGC 4 cut(s) 39, 42, 215, 278
AclWI GGATC 6 cut(s) 771, 942, 978, 1011, 1024, 1079
AcoI YGGCCR 1 cut(s) 1035
AcsI RAATTY 1 cut(s) 500
AcuI CTGAAG 3 cut(s) 466, 654, 939
AcvI CACGTG 1 cut(s) 811
AfaI GTAC 4 cut(s) 71, 273, 677, 916
AfiI CCNNNNNNNGG 2 cut(s) 257, 530
AflIII ACRYGT 1 cut(s) 808
AgsI TTSAA 5 cut(s) 199, 320, 497, 519, 646
AhlI ACTAGT 1 cut(s) 356
AjnI CCWGG 1 cut(s) 1003
AluBI AGCT 3 cut(s) 172, 188, 783
AluI AGCT 3 cut(s) 172, 188, 783
Alw26I GTCTC 4 cut(s) 148, 433, 439, 859
AlwI GGATC 6 cut(s) 771, 942, 978, 1011, 1024, 1079
Ama87I CYCGRG 3 cut(s) 429, 436, 778
AoxI GGCC 1 cut(s) 1035
ApeKI GCWGC 1 cut(s) 247
ApoI RAATTY 1 cut(s) 500
AspLEI GCGC 2 cut(s) 83, 448
AspS9I GGNCC 2 cut(s) 902, 976
AsuHPI GGTGA 1 cut(s) 520
AvaI CYCGRG 3 cut(s) 429, 436, 778
AvaII GGWCC 2 cut(s) 902, 976
BamHI GGATCC 1 cut(s) 1016
BbrPI CACGTG 1 cut(s) 811
BbsI GAAGAC 1 cut(s) 292
BbvI GCAGC 1 cut(s) 259
BccI CCATC 5 cut(s) 251, 643, 687, 959, 983
BceAI ACGGC 2 cut(s) 255, 1022
BciT130I CCWGG 1 cut(s) 1005
BclI TGATCA 1 cut(s) 964
BcoDI GTCTC 4 cut(s) 148, 433, 439, 859
BcuI ACTAGT 1 cut(s) 356
BfaI CTAG 2 cut(s) 264, 357
BfoI RGCGCY 1 cut(s) 449
BglII AGATCT 1 cut(s) 425
BisI GCNGC 2 cut(s) 40, 248
BlsI GCNGC 2 cut(s) 41, 249
BmcAI AGTACT 1 cut(s) 916
Bme1390I CCNGG 1 cut(s) 1005
Bme18I GGWCC 2 cut(s) 902, 976
BmeT110I CYCGRG 3 cut(s) 429, 436, 778
BmgT120I GGNCC 2 cut(s) 902, 976
BmiI GGNNCC 1 cut(s) 1018
BmrFI CCNGG 1 cut(s) 1005
BmsI GCATC 1 cut(s) 703
BpiI GAAGAC 1 cut(s) 292
BplI GAGNNNNNCTC 2 cut(s) 59, 91
BpuEI CTTGAG 2 cut(s) 19, 805
BsaAI YACGTR 1 cut(s) 811
BsaJI CCNNGG 3 cut(s) 489, 898, 972
BsaWI WCCGGW 1 cut(s) 530
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 530
Bse1I ACTGG 1 cut(s) 269
BseBI CCWGG 1 cut(s) 1005
BseDI CCNNGG 3 cut(s) 489, 898, 972
BseGI GGATG 2 cut(s) 473, 590
BseLI CCNNNNNNNGG 2 cut(s) 257, 530
BseMII CTCAG 1 cut(s) 320
BseNI ACTGG 1 cut(s) 269
BseRI GAGGAG 1 cut(s) 69
BseX3I CGGCCG 1 cut(s) 1035
BseXI GCAGC 1 cut(s) 259
Bsh1236I CGCG 1 cut(s) 81
Bsh1285I CGRYCG 1 cut(s) 1038
BshFI GGCC 1 cut(s) 1037
BsiEI CGRYCG 1 cut(s) 1038
BsiHKCI CYCGRG 3 cut(s) 429, 436, 778
BsiSI CCGG 2 cut(s) 9, 531
BslFI GGGAC 2 cut(s) 345, 359
BslI CCNNNNNNNGG 2 cut(s) 257, 530
BsmAI GTCTC 4 cut(s) 148, 433, 439, 859
BsmBI CGTCTC 3 cut(s) 148, 433, 859
BsmFI GGGAC 2 cut(s) 345, 359
BsnI GGCC 1 cut(s) 1037
BsoBI CYCGRG 3 cut(s) 429, 436, 778
Bsp19I CCATGG 3 cut(s) 489, 898, 972
BspACI CCGC 4 cut(s) 39, 42, 215, 278
BspANI GGCC 1 cut(s) 1037
BspCNI CTCAG 1 cut(s) 321
BspFNI CGCG 1 cut(s) 81
BspLI GGNNCC 1 cut(s) 1018
BspPI GGATC 6 cut(s) 771, 942, 978, 1011, 1024, 1079
BsrI ACTGG 1 cut(s) 269
BssECI CCNNGG 3 cut(s) 489, 898, 972
BssT1I CCWWGG 3 cut(s) 489, 898, 972
Bst2UI CCWGG 1 cut(s) 1005
Bst4CI ACNGT 4 cut(s) 142, 337, 395, 457
Bst6I CTCTTC 5 cut(s) 60, 416, 575, 862, 1081
BstBAI YACGTR 1 cut(s) 811
BstC8I GCNNGC 1 cut(s) 26
BstDEI CTNAG 2 cut(s) 329, 944
BstDSI CCRYGG 3 cut(s) 489, 898, 972
BstF5I GGATG 2 cut(s) 473, 590
BstFNI CGCG 1 cut(s) 81
BstH2I RGCGCY 1 cut(s) 449
BstHHI GCGC 2 cut(s) 83, 448
BstMAI GTCTC 4 cut(s) 148, 433, 439, 859
BstMCI CGRYCG 1 cut(s) 1038
BstMWI GCNNNNNNNGC 1 cut(s) 185
BstNI CCWGG 1 cut(s) 1005
BstNSI RCATGY 1 cut(s) 28
BstSCI CCNGG 1 cut(s) 1003
BstUI CGCG 1 cut(s) 81
BstV1I GCAGC 1 cut(s) 259
BstV2I GAAGAC 1 cut(s) 292
BstX2I RGATCY 5 cut(s) 425, 947, 983, 1016, 1071
BstYI RGATCY 5 cut(s) 425, 947, 983, 1016, 1071
BstZI CGGCCG 1 cut(s) 1035
BsuRI GGCC 1 cut(s) 1037
BtgI CCRYGG 3 cut(s) 489, 898, 972
BtgZI GCGATG 1 cut(s) 139
BtsCI GGATG 2 cut(s) 473, 590
Cac8I GCNNGC 1 cut(s) 26
CfoI GCGC 2 cut(s) 83, 448
Cfr13I GGNCC 2 cut(s) 902, 976
CseI GACGC 1 cut(s) 185
CsiI ACCWGGT 1 cut(s) 1003
Csp6I GTAC 4 cut(s) 70, 272, 676, 915
CviAII CATG 5 cut(s) 25, 490, 604, 899, 973
CviQI GTAC 4 cut(s) 70, 272, 676, 915
DdeI CTNAG 2 cut(s) 329, 944
EaeI YGGCCR 1 cut(s) 1035
EagI CGGCCG 1 cut(s) 1035
Eam1104I CTCTTC 5 cut(s) 60, 416, 575, 862, 1081
EarI CTCTTC 5 cut(s) 60, 416, 575, 862, 1081
EclXI CGGCCG 1 cut(s) 1035
Eco130I CCWWGG 3 cut(s) 489, 898, 972
Eco47I GGWCC 2 cut(s) 902, 976
Eco52I CGGCCG 1 cut(s) 1035
Eco57I CTGAAG 3 cut(s) 466, 654, 939
Eco72I CACGTG 1 cut(s) 811
Eco88I CYCGRG 3 cut(s) 429, 436, 778
EcoRI GAATTC 1 cut(s) 500
EcoRII CCWGG 1 cut(s) 1003
EcoT14I CCWWGG 3 cut(s) 489, 898, 972
ErhI CCWWGG 3 cut(s) 489, 898, 972
Esp3I CGTCTC 3 cut(s) 148, 433, 859
FaeI CATG 5 cut(s) 28, 493, 607, 902, 976
FaqI GGGAC 2 cut(s) 345, 359
FatI CATG 5 cut(s) 24, 489, 603, 898, 972
FbaI TGATCA 1 cut(s) 964
FblI GTMKAC 1 cut(s) 233
Fnu4HI GCNGC 2 cut(s) 40, 248
FokI GGATG 2 cut(s) 460, 577
Fsp4HI GCNGC 2 cut(s) 40, 248
FspBI CTAG 2 cut(s) 264, 357
GlaI GCGC 2 cut(s) 82, 447
GluI GCNGC 2 cut(s) 40, 248
HaeII RGCGCY 1 cut(s) 449
HaeIII GGCC 1 cut(s) 1037
HapII CCGG 2 cut(s) 9, 531
HgaI GACGC 1 cut(s) 185
HhaI GCGC 2 cut(s) 83, 448
Hin1II CATG 5 cut(s) 28, 493, 607, 902, 976
Hin6I GCGC 2 cut(s) 81, 446
HinP1I GCGC 2 cut(s) 81, 446
HinfI GANTC 6 cut(s) 5, 74, 412, 432, 646, 700
HpaII CCGG 2 cut(s) 9, 531
HphI GGTGA 1 cut(s) 520
Hpy166II GTNNAC 2 cut(s) 234, 814
Hpy188I TCNGA 8 cut(s) 292, 330, 485, 673, 699, 988, 1021, 1084
Hpy188III TCNNGA 9 cut(s) 62, 264, 429, 436, 438, 497, 558, 597, 1014
Hpy8I GTNNAC 2 cut(s) 234, 814
Hpy99I CGWCG 2 cut(s) 643, 871
HpyAV CCTTC 4 cut(s) 525, 668, 675, 1043
HpyCH4III ACNGT 4 cut(s) 142, 337, 395, 457
HpyCH4IV ACGT 3 cut(s) 641, 810, 816
HpyCH4V TGCA 1 cut(s) 28
HpyF10VI GCNNNNNNNGC 1 cut(s) 185
HpyF3I CTNAG 2 cut(s) 329, 944
HpySE526I ACGT 3 cut(s) 641, 810, 816
Hsp92II CATG 5 cut(s) 28, 493, 607, 902, 976
HspAI GCGC 2 cut(s) 81, 446
Ksp22I TGATCA 1 cut(s) 964
LpnPI CCDG 7 cut(s) 22, 282, 544, 918, 990, 999, 1017
Lsp1109I GCAGC 1 cut(s) 259
LweI GCATC 1 cut(s) 703
MabI ACCWGGT 1 cut(s) 1003
MaeI CTAG 2 cut(s) 264, 357
MaeII ACGT 3 cut(s) 641, 810, 816
MaeIII GTNAC 1 cut(s) 992
MflI RGATCY 5 cut(s) 425, 947, 983, 1016, 1071
MluCI AATT 3 cut(s) 93, 500, 1061
MlyI GAGTC 2 cut(s) 68, 441
MmeI TCCRAC 3 cut(s) 188, 501, 799
MnlI CCTC 8 cut(s) 43, 47, 234, 604, 627, 672, 787, 826
MseI TTAA 3 cut(s) 108, 711, 758
MspA1I CMGCKG 1 cut(s) 215
MspI CCGG 2 cut(s) 9, 531
MspR9I CCNGG 1 cut(s) 1005
MvaI CCWGG 1 cut(s) 1005
MvnI CGCG 1 cut(s) 81
MwoI GCNNNNNNNGC 1 cut(s) 185
NcoI CCATGG 3 cut(s) 489, 898, 972
NlaIII CATG 5 cut(s) 28, 493, 607, 902, 976
NlaIV GGNNCC 1 cut(s) 1018
NspI RCATGY 1 cut(s) 28
PaeI GCATGC 1 cut(s) 28
PaeR7I CTCGAG 3 cut(s) 429, 436, 778
PcsI WCGNNNNNNNCGW 1 cut(s) 767
PfeI GAWTC 4 cut(s) 5, 412, 646, 700
PkrI GCNGC 2 cut(s) 41, 249
PleI GAGTC 2 cut(s) 68, 440
PmaCI CACGTG 1 cut(s) 811
PmlI CACGTG 1 cut(s) 811
PpsI GAGTC 2 cut(s) 68, 440
Ppu21I YACGTR 1 cut(s) 811
Psp6I CCWGG 1 cut(s) 1003
PspCI CACGTG 1 cut(s) 811
PspGI CCWGG 1 cut(s) 1003
PspN4I GGNNCC 1 cut(s) 1018
PspPI GGNCC 2 cut(s) 902, 976
PspXI VCTCGAGB 1 cut(s) 778
PsuI RGATCY 5 cut(s) 425, 947, 983, 1016, 1071
RsaI GTAC 4 cut(s) 71, 273, 677, 916
RsaNI GTAC 4 cut(s) 70, 272, 676, 915
SaqAI TTAA 3 cut(s) 108, 711, 758
SatI GCNGC 2 cut(s) 40, 248
Sau96I GGNCC 2 cut(s) 902, 976
ScaI AGTACT 1 cut(s) 916
SchI GAGTC 2 cut(s) 68, 441
ScrFI CCNGG 1 cut(s) 1005
SetI ASST 9 cut(s) 174, 190, 644, 660, 785, 813, 819, 1006, 1054
SexAI ACCWGGT 1 cut(s) 1003
SfaNI GCATC 1 cut(s) 703
Sfr274I CTCGAG 3 cut(s) 429, 436, 778
SinI GGWCC 2 cut(s) 902, 976
SlaI CTCGAG 3 cut(s) 429, 436, 778
SmlI CTYRAG 5 cut(s) 34, 429, 436, 778, 784
SmoI CTYRAG 5 cut(s) 34, 429, 436, 778, 784
SpeI ACTAGT 1 cut(s) 356
SphI GCATGC 1 cut(s) 28
Sse9I AATT 3 cut(s) 93, 500, 1061
SsiI CCGC 4 cut(s) 39, 42, 215, 278
SspMI CTAG 2 cut(s) 264, 357
StyD4I CCNGG 1 cut(s) 1003
StyI CCWWGG 3 cut(s) 489, 898, 972
TaaI ACNGT 4 cut(s) 142, 337, 395, 457
TaiI ACGT 3 cut(s) 644, 813, 819
TaqI TCGA 5 cut(s) 430, 437, 638, 779, 869
TasI AATT 3 cut(s) 93, 500, 1061
TatI WGTACW 1 cut(s) 914
TauI GCSGC 1 cut(s) 42
TfiI GAWTC 4 cut(s) 5, 412, 646, 700
Tru1I TTAA 3 cut(s) 108, 711, 758
Tru9I TTAA 3 cut(s) 108, 711, 758
TseI GCWGC 1 cut(s) 247
TspDTI ATGAA 5 cut(s) 531, 579, 620, 731, 852
TspGWI ACGGA 3 cut(s) 361, 424, 776
VpaK11BI GGWCC 2 cut(s) 902, 976
XapI RAATTY 1 cut(s) 500
XbaI TCTAGA 1 cut(s) 263
XceI RCATGY 1 cut(s) 28
XcmI CCANNNNNNNNNTGG 1 cut(s) 986
XhoI CTCGAG 3 cut(s) 429, 436, 778
XmiI GTMKAC 1 cut(s) 233
XspI CTAG 2 cut(s) 264, 357
ZrmI AGTACT 1 cut(s) 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.