FvH4_1g21110

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012018
Physical Location & Seq
Reverse (-)
13141660 .. 13144258
2599 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g21110.t1

Sequence Viewer

Length: 1383 bp
ATGGATTCCGGTAACAGCGGGAGTATGCAGTCCTCCAGCGGCGGCGACGACGAGTCGTACGATTCACGCGCGGAGTCGATCTCGGCCTTGCTGGGTCCCACCATGACTAACCACCACCACCACCAACACCCACCGCAAGTAGCGCAACACCACCAACAACAACAGACACAACACCACCGCCATCATCCTCATCACCATGTTGAGTTCGACACCTTATCCAACATCTTCGATTCGAGGCTCACGAACCCGAACCCGCTCCTCAACCTCGACATGGTGTGGTCCAAGACGATGAGATCCGACCAGAGTATGGCCGATCTTGGCGGCCAGAACGACCTCAGCCAACCCTTTTTGACCAATAGTCAACTAGGAGGACAAGTCAGTAGAGGAGGCGGCGGCGGCGCTGGAGGAGGGTCTTCTACTTTTACTCAGATCCCATCATCGTCAGCTCCGAACGACCAAAGCCTCAACAACAACAACGTCGGCGGTGTTCGGAACACCAACCCGAAAAAGAGGTCGAGAGCGTCGAGGCGTGCGCCCACTACGGTTCTCACAACAGACACAACAAACTTCCGAGCCATGGTTCAAGAATTTACAGGTATTCCTGCACCCCCATTTACGAGTTCGCCTTTTCCGAGAAGCAGGTTGGATTTGTTTGGATCTTCGGCTTCCTCTATGGTTAGATCGGGTCCGGGTCAGAATCATACAGACCCTTCATCTCTTCCTCCTTATCTTTTGAGACCATTTCCTCACAAAGCTCCACTACTACCTTCTCCTTCATCTCCGTCTTTGTTGCCTTCTAGTGATTCAAATGCTAATAGTAATATTGGTGGTTCTAATCTACAACAGTCTCAGAGTCTTTTGAACATGAACAACATGCAAAACTCTTCCTCCTCATCAGTTCTGAATTTTCAGTCCCTCTTTCAAAACCAGCAGCAGCCTCAGTCGAATTTACAAAAATACCCATTACCCGGTTCAGGCAATATTCTTGGCTCCTCCAAGTCATCACAAGGCTCTTTGGGTGTGCTTGAAGATCAGTTTGGTTTGAGCCATGGGCTAAATGTGCAGCAGCTTCCTAACATTGTGTCGTCGTCGTCCTCAGACGGTGGCCTCACGCTTCAGCAGCAGCAACCTAGGCACGACAATAATCCGAGGACGACGACGACGACTACAATGAACTGGGTTGACAACAACAACAAGAGCGACGGCAATGTCGATCTTCTGCGGTCCGTCAATGGGAATTACGGTAATGGAAAATTAAACTACGCTGCTTCTTCTTCGTCGAATGTCGTCGATAAAGCTGGGGTGCAGCTGGAGAATAATGTGAGTGCTACTACAGCTAGAAGTGAAGGTATGGTGGAATCATGGATTTGCTCATCTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

461

Amino Acids

49.18

Weight (kDa)

6.65

Isoelectric Point (pI)

67.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 177 - 204 1.3e-11 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012018)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65170 AT5G65170
fragaria_vesca FvH4_1g21110
malus_domestica MD01G1027700.v1.1 MD15G1325900.v1.1
prunus_persica Prupe.6G183200_v2.0.a1 Prupe.6G183200_v2.0.a1
pyrus_communis pycom01g05580 pycom15g28700
rosa_chinensis RchiOBHm_Chr2g0114891
rosa_laevigata RLG00000018131
rosa_multiflora Rmu_sc0002764.1_g000028
rosa_roxburghii Rroxscaffold_2G00130390
rosa_rugosa Rorug02G0191400
rosa_samantha Rh2AG250000 Rh2BG259600 Rh2CG254400 Rh2DG258200
rosa_wichuraiana Rw2G019500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1208
Acc36I ACCTGC 1 cut(s) 630
AccB7I CCANNNNNTGG 1 cut(s) 307
AccBSI CCGCTC 1 cut(s) 256
AccII CGCG 2 cut(s) 69, 71
AclWI GGATC 3 cut(s) 288, 424, 664
AcoI YGGCCR 2 cut(s) 309, 322
AcsI RAATTY 3 cut(s) 587, 904, 946
AcuI CTGAAG 1 cut(s) 1100
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 6 cut(s) 271, 307, 577, 968, 974, 1233
AgsI TTSAA 5 cut(s) 584, 807, 862, 923, 1028
AhdI GACNNNNNGTC 2 cut(s) 52, 357
AjuI GAANNNNNNNTTGG 2 cut(s) 1020, 1052
AluBI AGCT 6 cut(s) 446, 755, 1069, 1298, 1309, 1337
AluI AGCT 6 cut(s) 446, 755, 1069, 1298, 1309, 1337
Alw26I GTCTC 2 cut(s) 730, 852
AlwI GGATC 3 cut(s) 288, 424, 664
AoxI GGCC 4 cut(s) 84, 309, 322, 1105
ApeKI GCWGC 8 cut(s) 931, 934, 1063, 1066, 1120, 1123, 1265, 1306
ApoI RAATTY 3 cut(s) 587, 904, 946
ArsI GACNNNNNNTTYG 2 cut(s) 497, 529
AspA2I CCTAGG 1 cut(s) 1130
AspLEI GCGC 4 cut(s) 71, 145, 401, 535
AspS9I GGNCC 4 cut(s) 95, 279, 686, 1224
AsuC2I CCSGG 2 cut(s) 690, 969
AsuHPI GGTGA 1 cut(s) 185
AvaII GGWCC 4 cut(s) 95, 279, 686, 1224
AvrII CCTAGG 1 cut(s) 1130
BbsI GAAGAC 1 cut(s) 405
BbvCI CCTCAGC 1 cut(s) 335
BbvI GCAGC 8 cut(s) 943, 946, 1075, 1078, 1132, 1135, 1252, 1318
BccI CCATC 2 cut(s) 189, 442
BceAI ACGGC 1 cut(s) 1219
BcnI CCSGG 2 cut(s) 690, 969
BcoDI GTCTC 2 cut(s) 730, 852
BfaI CTAG 4 cut(s) 365, 798, 1131, 1338
BfmI CTRYAG 1 cut(s) 1332
BfoI RGCGCY 1 cut(s) 402
BfuAI ACCTGC 1 cut(s) 630
BlnI CCTAGG 1 cut(s) 1130
Bme1390I CCNGG 2 cut(s) 690, 969
Bme18I GGWCC 4 cut(s) 95, 279, 686, 1224
BmeRI GACNNNNNGTC 2 cut(s) 52, 357
BmgT120I GGNCC 4 cut(s) 95, 279, 686, 1224
BmiI GGNNCC 4 cut(s) 96, 97, 687, 991
BmrFI CCNGG 2 cut(s) 690, 969
BmrI ACTGGG 1 cut(s) 1186
BmuI ACTGGG 1 cut(s) 1186
BpiI GAAGAC 1 cut(s) 405
BplI GAGNNNNNCTC 2 cut(s) 65, 97
BpmI CTGGAG 3 cut(s) 19, 423, 1331
Bpu10I CCTNAGC 1 cut(s) 335
BpuMI CCSGG 2 cut(s) 690, 969
BsaI GGTCTC 1 cut(s) 730
BsaJI CCNNGG 4 cut(s) 576, 1048, 1130, 1148
BsaWI WCCGGW 1 cut(s) 8
BsaXI ACNNNNNCTCC 4 cut(s) 360, 390, 872, 902
Bsc4I CCNNNNNNNGG 6 cut(s) 271, 307, 577, 968, 974, 1233
Bse1I ACTGG 1 cut(s) 1181
Bse3DI GCAATG 1 cut(s) 1213
BseDI CCNNGG 4 cut(s) 576, 1048, 1130, 1148
BseGI GGATG 1 cut(s) 184
BseLI CCNNNNNNNGG 6 cut(s) 271, 307, 577, 968, 974, 1233
BseMI GCAATG 1 cut(s) 1213
BseMII CTCAG 5 cut(s) 349, 440, 863, 953, 1110
BseNI ACTGG 1 cut(s) 1181
BseRI GAGGAG 5 cut(s) 248, 399, 420, 880, 982
BseXI GCAGC 8 cut(s) 943, 946, 1075, 1078, 1132, 1135, 1252, 1318
BseYI CCCAGC 2 cut(s) 91, 1298
BsgI GTGCAG 3 cut(s) 588, 1082, 1325
Bsh1236I CGCG 2 cut(s) 69, 71
BshFI GGCC 4 cut(s) 86, 311, 324, 1107
BsiSI CCGG 3 cut(s) 9, 689, 969
BsiWI CGTACG 1 cut(s) 57
BslFI GGGAC 2 cut(s) 81, 898
BslI CCNNNNNNNGG 6 cut(s) 271, 307, 577, 968, 974, 1233
BsmAI GTCTC 2 cut(s) 730, 852
BsmFI GGGAC 2 cut(s) 81, 898
BsnI GGCC 4 cut(s) 86, 311, 324, 1107
Bso31I GGTCTC 1 cut(s) 730
Bsp143I GATC 8 cut(s) 78, 293, 313, 429, 656, 680, 1030, 1213
Bsp19I CCATGG 2 cut(s) 576, 1048
BspANI GGCC 4 cut(s) 86, 311, 324, 1107
BspCNI CTCAG 5 cut(s) 348, 439, 862, 952, 1109
BspFNI CGCG 2 cut(s) 69, 71
BspLI GGNNCC 4 cut(s) 96, 97, 687, 991
BspMI ACCTGC 1 cut(s) 630
BspPI GGATC 3 cut(s) 288, 424, 664
BspTNI GGTCTC 1 cut(s) 730
BsrBI CCGCTC 1 cut(s) 256
BsrDI GCAATG 1 cut(s) 1213
BsrI ACTGG 1 cut(s) 1181
BssECI CCNNGG 4 cut(s) 576, 1048, 1130, 1148
BssMI GATC 8 cut(s) 78, 293, 313, 429, 656, 680, 1030, 1213
BssT1I CCWWGG 3 cut(s) 576, 1048, 1130
Bst4CI ACNGT 4 cut(s) 544, 846, 1103, 1244
Bst6I CTCTTC 2 cut(s) 723, 889
BstC8I GCNNGC 1 cut(s) 531
BstDEI CTNAG 5 cut(s) 335, 426, 849, 939, 1096
BstDSI CCRYGG 2 cut(s) 576, 1048
BstF5I GGATG 1 cut(s) 184
BstFNI CGCG 2 cut(s) 69, 71
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 4 cut(s) 71, 145, 401, 535
BstKTI GATC 8 cut(s) 81, 296, 316, 432, 659, 683, 1033, 1216
BstMAI GTCTC 2 cut(s) 730, 852
BstMBI GATC 8 cut(s) 78, 293, 313, 429, 656, 680, 1030, 1213
BstMWI GCNNNNNNNGC 6 cut(s) 142, 396, 1060, 1120, 1132, 1334
BstNSI RCATGY 1 cut(s) 877
BstSCI CCNGG 2 cut(s) 688, 967
BstSFI CTRYAG 1 cut(s) 1332
BstUI CGCG 2 cut(s) 69, 71
BstV1I GCAGC 8 cut(s) 943, 946, 1075, 1078, 1132, 1135, 1252, 1318
BstV2I GAAGAC 1 cut(s) 405
BstX2I RGATCY 3 cut(s) 293, 429, 656
BstYI RGATCY 3 cut(s) 293, 429, 656
BsuRI GGCC 4 cut(s) 86, 311, 324, 1107
BtgI CCRYGG 2 cut(s) 576, 1048
BtsCI GGATG 1 cut(s) 184
BveI ACCTGC 1 cut(s) 630
Cac8I GCNNGC 1 cut(s) 531
CfoI GCGC 4 cut(s) 71, 145, 401, 535
Cfr13I GGNCC 4 cut(s) 95, 279, 686, 1224
CpoI CGGWCCG 1 cut(s) 1224
CseI GACGC 1 cut(s) 510
Csp6I GTAC 1 cut(s) 58
CspI CGGWCCG 1 cut(s) 1224
CviAII CATG 8 cut(s) 103, 197, 271, 577, 865, 874, 1049, 1362
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 5 cut(s) 335, 426, 849, 939, 1096
DpnI GATC 8 cut(s) 80, 295, 315, 431, 658, 682, 1032, 1215
DpnII GATC 8 cut(s) 78, 293, 313, 429, 656, 680, 1030, 1213
DrdI GACNNNNNNGTC 1 cut(s) 1208
DriI GACNNNNNGTC 2 cut(s) 52, 357
DseDI GACNNNNNNGTC 1 cut(s) 1208
EaeI YGGCCR 2 cut(s) 309, 322
Eam1104I CTCTTC 2 cut(s) 723, 889
Eam1105I GACNNNNNGTC 2 cut(s) 52, 357
EarI CTCTTC 2 cut(s) 723, 889
Eco130I CCWWGG 3 cut(s) 576, 1048, 1130
Eco31I GGTCTC 1 cut(s) 730
Eco47I GGWCC 4 cut(s) 95, 279, 686, 1224
Eco57I CTGAAG 1 cut(s) 1100
EcoO109I RGGNCCY 1 cut(s) 95
EcoT14I CCWWGG 3 cut(s) 576, 1048, 1130
ErhI CCWWGG 3 cut(s) 576, 1048, 1130
FaeI CATG 8 cut(s) 106, 200, 274, 580, 868, 877, 1052, 1365
FaqI GGGAC 2 cut(s) 81, 898
FatI CATG 8 cut(s) 102, 196, 270, 576, 864, 873, 1048, 1361
FauI CCCGC 2 cut(s) 11, 261
FokI GGATG 1 cut(s) 171
FspBI CTAG 4 cut(s) 365, 798, 1131, 1338
GlaI GCGC 4 cut(s) 70, 144, 400, 534
GsaI CCCAGC 2 cut(s) 95, 1302
GsuI CTGGAG 3 cut(s) 19, 423, 1331
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 4 cut(s) 86, 311, 324, 1107
HapII CCGG 3 cut(s) 9, 689, 969
HgaI GACGC 1 cut(s) 510
HhaI GCGC 4 cut(s) 71, 145, 401, 535
Hin1II CATG 8 cut(s) 106, 200, 274, 580, 868, 877, 1052, 1365
Hin6I GCGC 4 cut(s) 69, 143, 399, 533
HinP1I GCGC 4 cut(s) 69, 143, 399, 533
HincII GTYRAC 2 cut(s) 362, 1183
HindII GTYRAC 2 cut(s) 362, 1183
HinfI GANTC 9 cut(s) 5, 53, 62, 74, 230, 697, 803, 853, 1358
HpaII CCGG 3 cut(s) 9, 689, 969
HphI GGTGA 1 cut(s) 185
Hpy166II GTNNAC 2 cut(s) 362, 1183
Hpy188III TCNNGA 3 cut(s) 241, 516, 584
Hpy8I GTNNAC 2 cut(s) 362, 1183
HpyAV CCTTC 5 cut(s) 720, 777, 783, 804, 1340
HpyCH4III ACNGT 4 cut(s) 544, 846, 1103, 1244
HpyCH4IV ACGT 1 cut(s) 477
HpyCH4V TGCA 5 cut(s) 28, 605, 877, 1063, 1306
HpyF10VI GCNNNNNNNGC 6 cut(s) 142, 396, 1060, 1120, 1132, 1334
HpyF3I CTNAG 5 cut(s) 335, 426, 849, 939, 1096
HpySE526I ACGT 1 cut(s) 477
Hsp92II CATG 8 cut(s) 106, 200, 274, 580, 868, 877, 1052, 1365
HspAI GCGC 4 cut(s) 69, 143, 399, 533
KflI GGGWCCC 1 cut(s) 95
Kzo9I GATC 8 cut(s) 78, 293, 313, 429, 656, 680, 1030, 1213
LmnI GCTCC 4 cut(s) 261, 451, 760, 995
Lsp1109I GCAGC 8 cut(s) 943, 946, 1075, 1078, 1132, 1135, 1252, 1318
MaeI CTAG 4 cut(s) 365, 798, 1131, 1338
MaeII ACGT 1 cut(s) 477
MaeIII GTNAC 1 cut(s) 11
MalI GATC 8 cut(s) 80, 295, 315, 431, 658, 682, 1032, 1215
MbiI CCGCTC 1 cut(s) 256
MboI GATC 8 cut(s) 78, 293, 313, 429, 656, 680, 1030, 1213
MboII GAAGA 9 cut(s) 217, 405, 651, 710, 876, 1040, 1208, 1263, 1266
MflI RGATCY 3 cut(s) 293, 429, 656
MluCI AATT 5 cut(s) 587, 904, 946, 1237, 1253
MlyI GAGTC 3 cut(s) 62, 83, 862
MmeI TCCRAC 3 cut(s) 243, 321, 624
MseI TTAA 2 cut(s) 1256, 1381
MslI CAYNNNNRTG 1 cut(s) 195
MspA1I CMGCKG 3 cut(s) 18, 39, 1309
MspI CCGG 3 cut(s) 9, 689, 969
MspR9I CCNGG 2 cut(s) 690, 969
MvnI CGCG 2 cut(s) 69, 71
MwoI GCNNNNNNNGC 6 cut(s) 142, 396, 1060, 1120, 1132, 1334
NciI CCSGG 2 cut(s) 690, 969
NcoI CCATGG 2 cut(s) 576, 1048
NdeII GATC 8 cut(s) 78, 293, 313, 429, 656, 680, 1030, 1213
NlaIII CATG 8 cut(s) 106, 200, 274, 580, 868, 877, 1052, 1365
NlaIV GGNNCC 4 cut(s) 96, 97, 687, 991
NmeAIII GCCGAG 1 cut(s) 62
NspI RCATGY 1 cut(s) 877
PcsI WCGNNNNNNNCGW 7 cut(s) 57, 239, 446, 521, 629, 1160, 1209
PfeI GAWTC 6 cut(s) 5, 62, 230, 697, 803, 1358
Pfl23II CGTACG 1 cut(s) 57
PflMI CCANNNNNTGG 1 cut(s) 307
PleI GAGTC 3 cut(s) 61, 82, 861
PpsI GAGTC 3 cut(s) 61, 82, 861
PpuMI RGGWCCY 1 cut(s) 95
Psp5II RGGWCCY 1 cut(s) 95
PspFI CCCAGC 2 cut(s) 91, 1298
PspLI CGTACG 1 cut(s) 57
PspN4I GGNNCC 4 cut(s) 96, 97, 687, 991
PspPI GGNCC 4 cut(s) 95, 279, 686, 1224
PspPPI RGGWCCY 1 cut(s) 95
PsuI RGATCY 3 cut(s) 293, 429, 656
PvuII CAGCTG 1 cut(s) 1309
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
RseI CAYNNNNRTG 1 cut(s) 195
Rsr2I CGGWCCG 1 cut(s) 1224
RsrII CGGWCCG 1 cut(s) 1224
SaqAI TTAA 2 cut(s) 1256, 1381
Sau3AI GATC 8 cut(s) 78, 293, 313, 429, 656, 680, 1030, 1213
Sau96I GGNCC 4 cut(s) 95, 279, 686, 1224
SchI GAGTC 3 cut(s) 62, 83, 862
ScrFI CCNGG 2 cut(s) 690, 969
SfcI CTRYAG 1 cut(s) 1332
SinI GGWCC 4 cut(s) 95, 279, 686, 1224
SmiMI CAYNNNNRTG 1 cut(s) 195
Sse9I AATT 5 cut(s) 587, 904, 946, 1237, 1253
SspI AATATT 2 cut(s) 823, 982
SspMI CTAG 4 cut(s) 365, 798, 1131, 1338
StyD4I CCNGG 2 cut(s) 688, 967
StyI CCWWGG 3 cut(s) 576, 1048, 1130
TaaI ACNGT 4 cut(s) 544, 846, 1103, 1244
TaiI ACGT 1 cut(s) 480
TasI AATT 5 cut(s) 587, 904, 946, 1237, 1253
TauI GCSGC 6 cut(s) 42, 45, 324, 393, 396, 399
TfiI GAWTC 6 cut(s) 5, 62, 230, 697, 803, 1358
Tru1I TTAA 2 cut(s) 1256, 1381
Tru9I TTAA 2 cut(s) 1256, 1381
TseI GCWGC 8 cut(s) 931, 934, 1063, 1066, 1120, 1123, 1265, 1306
TspDTI ATGAA 4 cut(s) 702, 765, 881, 1187
TspGWI ACGGA 2 cut(s) 771, 1216
Van91I CCANNNNNTGG 1 cut(s) 307
VpaK11BI GGWCC 4 cut(s) 95, 279, 686, 1224
XapI RAATTY 3 cut(s) 587, 904, 946
XceI RCATGY 1 cut(s) 877
XmaJI CCTAGG 1 cut(s) 1130
XspI CTAG 4 cut(s) 365, 798, 1131, 1338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.