Rroxscaffold_2G00130390

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012018
Physical Location & Seq
Reverse (-)
66533794 .. 66535148
1355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00130390.1

Sequence Viewer

Length: 1311 bp
ATGGATTCCGGTAACAGCGGAAGTATGCAGTCCTCGAGCGGCGGCGACGACGAGTCGTACGACTCACGCGCGGAGTCGATCTCGGCCTTGCTGGGTCCCACCATGACCAACCACCACCACCACCACCACCCACCGCAACAACAACAGCAACAACACCACCGCCATCATCCTCATGTTGAGTTCGACACCTTATCCAACATCTTCGATTCGAGGCTCACAAACTCGAACCCGCTCCTCAACCTCGACATGGTGTGGTCCAAGACGCTGAGATCCGACCCGAGTATGTCCGATCTTGGCGGCCAGAACGAGCTCAGCCAATCCTTTTTGACCAATAGTCAACTAGGAGGACAAGTCAGCAGAACAGGAGTCGGCGGTGGCGGAGGAGGGTCATCTACTTTTACTCAGATCCCATCCTCGTCAGCTCCGAACGACCAGACCACCACCAACAACGTCGGCGGCGTTCGGAACAGCAACCCAAAAAGAGACACAACAAACTTCCGAGCCATGGTTCAAGAATTTACAGGTATTCCTGCACCCCCATTTACGAGTTCGCCTTTTCCTAGAAGCAGGTTGGATTTGTTCGGGTCTTCAGCTTCATCTGTGGTTAGATCGGGCCCGGGTCCCAATCATATGGATCCTTCTTCTCTTCCTTCTTATCTGTTGAGACCATTTGCTCATAAAGCTCCACTACTACCTTCTCCTCCATCTCCATCTTTGTTACCCTCTAGTAATTCAAATACTAATAGTAATATTGGTGGTTCTAATCTACAACAGTCTCAGAGTCTTTTGAACATGAACAACATGCAAAACTCCTCCTCTTCATCAGTTCTGAATTTTCACTCCCTCTTTCAAAACCAGCAGCCTCAGCCGAATTTACCAAAATACCCATTACCCGGTTCAGGCAATATTCTTGGCTCCTCCAAGTCTCAAGGCTCTTTGGGTGTGCTTGAAGATCAGTTTGGTTTGAGCCATGGGCTAAATGTGCAGCTTCCTAACATCGTGTCGTCGTCGTCCTCGGATGGTGGCCGTACGCTTCAGCAGCAGCAACCTAGGCACGACAATAACCAGAGGACGACAACGACGACAATGAACTGGGTTGACAACAACAACAACAACAACAACAAGAACGATGGCAATGTCGATCTTCTGAGGTCCGTCAATGGGAATTACAGTAATGGCAAACTAAACTACGCGGCTTCTTCTTCGTCGAATGTTGTCGATAAAGCTGGGGTGCAGCTGGAGAATAATGTGAGTACTACTACAGCTAGAAGTGAAGGTATGGTGGAATCATGGATTTGCTCTTCAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

436

Amino Acids

46.64

Weight (kDa)

6.33

Isoelectric Point (pI)

63.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 162 - 180 5.6e-07 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012018)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65170 AT5G65170
fragaria_vesca FvH4_1g21110
malus_domestica MD01G1027700.v1.1 MD15G1325900.v1.1
prunus_persica Prupe.6G183200_v2.0.a1 Prupe.6G183200_v2.0.a1
pyrus_communis pycom01g05580 pycom15g28700
rosa_chinensis RchiOBHm_Chr2g0114891
rosa_laevigata RLG00000018131
rosa_multiflora Rmu_sc0002764.1_g000028
rosa_roxburghii Rroxscaffold_2G00130390
rosa_rugosa Rorug02G0191400
rosa_samantha Rh2AG250000 Rh2BG259600 Rh2CG254400 Rh2DG258200
rosa_wichuraiana Rw2G019500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 558
AccBSI CCGCTC 2 cut(s) 39, 232
AccII CGCG 3 cut(s) 69, 71, 1193
AclWI GGATC 4 cut(s) 264, 400, 629, 642
AcoI YGGCCR 2 cut(s) 298, 1024
AcsI RAATTY 3 cut(s) 515, 832, 871
AcuI CTGAAG 3 cut(s) 573, 1019, 1287
AfaI GTAC 3 cut(s) 59, 1030, 1255
AfiI CCNNNNNNNGG 5 cut(s) 247, 505, 893, 899, 1161
AgsI TTSAA 5 cut(s) 512, 735, 790, 851, 950
AhdI GACNNNNNGTC 2 cut(s) 52, 333
AjuI GAANNNNNNNTTGG 2 cut(s) 942, 974
AluBI AGCT 8 cut(s) 310, 422, 593, 683, 988, 1226, 1237, 1265
AluI AGCT 8 cut(s) 310, 422, 593, 683, 988, 1226, 1237, 1265
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 4 cut(s) 477, 658, 780, 930
AlwI GGATC 4 cut(s) 264, 400, 629, 642
Ama87I CYCGRG 3 cut(s) 34, 277, 616
AoxI GGCC 4 cut(s) 84, 298, 613, 1024
ApaI GGGCCC 1 cut(s) 617
ApeKI GCWGC 5 cut(s) 859, 985, 1039, 1042, 1234
ApoI RAATTY 3 cut(s) 515, 832, 871
AspA2I CCTAGG 1 cut(s) 1049
AspLEI GCGC 1 cut(s) 71
AspS9I GGNCC 6 cut(s) 95, 255, 613, 614, 620, 1152
AsuC2I CCSGG 3 cut(s) 617, 618, 894
AvaI CYCGRG 3 cut(s) 34, 277, 616
AvaII GGWCC 4 cut(s) 95, 255, 620, 1152
AvrII CCTAGG 1 cut(s) 1049
BaeGI GKGCMC 1 cut(s) 617
BamHI GGATCC 1 cut(s) 634
BanII GRGCYC 2 cut(s) 312, 617
BbsI GAAGAC 1 cut(s) 579
Bbv12I GWGCWC 1 cut(s) 312
BbvCI CCTCAGC 1 cut(s) 864
BbvI GCAGC 5 cut(s) 871, 997, 1051, 1054, 1246
BccI CCATC 6 cut(s) 171, 418, 712, 718, 1013, 1124
BceAI ACGGC 1 cut(s) 1011
BcnI CCSGG 3 cut(s) 617, 618, 894
BcoDI GTCTC 4 cut(s) 477, 658, 780, 930
BfaI CTAG 5 cut(s) 341, 561, 726, 1050, 1266
BfmI CTRYAG 1 cut(s) 1260
BfuAI ACCTGC 1 cut(s) 558
BlnI CCTAGG 1 cut(s) 1049
BlpI GCTNAGC 1 cut(s) 311
BmcAI AGTACT 1 cut(s) 1255
Bme1390I CCNGG 3 cut(s) 617, 618, 894
Bme18I GGWCC 4 cut(s) 95, 255, 620, 1152
BmeRI GACNNNNNGTC 2 cut(s) 52, 333
BmeT110I CYCGRG 3 cut(s) 34, 277, 616
BmgT120I GGNCC 6 cut(s) 95, 255, 613, 614, 620, 1152
BmiI GGNNCC 7 cut(s) 96, 97, 615, 621, 622, 636, 916
BmrFI CCNGG 3 cut(s) 617, 618, 894
BmrI ACTGGG 1 cut(s) 1102
BmuI ACTGGG 1 cut(s) 1102
BpiI GAAGAC 1 cut(s) 579
BplI GAGNNNNNCTC 2 cut(s) 65, 97
BpmI CTGGAG 1 cut(s) 1259
Bpu10I CCTNAGC 1 cut(s) 864
Bpu1102I GCTNAGC 1 cut(s) 311
BpuEI CTTGAG 1 cut(s) 912
BpuMI CCSGG 3 cut(s) 617, 618, 894
BsaI GGTCTC 1 cut(s) 658
BsaJI CCNNGG 5 cut(s) 504, 616, 970, 1014, 1049
BsaWI WCCGGW 1 cut(s) 8
BsaXI ACNNNNNCTCC 2 cut(s) 336, 366
Bsc4I CCNNNNNNNGG 5 cut(s) 247, 505, 893, 899, 1161
Bse1I ACTGG 1 cut(s) 1097
Bse3DI GCAATG 1 cut(s) 1141
BseDI CCNNGG 5 cut(s) 504, 616, 970, 1014, 1049
BseGI GGATG 3 cut(s) 166, 410, 1024
BseLI CCNNNNNNNGG 5 cut(s) 247, 505, 893, 899, 1161
BseMI GCAATG 1 cut(s) 1141
BseMII CTCAG 6 cut(s) 257, 325, 416, 791, 878, 1139
BseNI ACTGG 1 cut(s) 1097
BseRI GAGGAG 6 cut(s) 224, 396, 690, 802, 805, 907
BseSI GKGCMC 1 cut(s) 617
BseXI GCAGC 5 cut(s) 871, 997, 1051, 1054, 1246
BseYI CCCAGC 2 cut(s) 91, 1226
BsgI GTGCAG 3 cut(s) 516, 1004, 1253
Bsh1236I CGCG 3 cut(s) 69, 71, 1193
BshFI GGCC 4 cut(s) 86, 300, 615, 1026
BsiHKAI GWGCWC 1 cut(s) 312
BsiHKCI CYCGRG 3 cut(s) 34, 277, 616
BsiSI CCGG 3 cut(s) 9, 617, 894
BsiWI CGTACG 2 cut(s) 57, 1028
BslFI GGGAC 2 cut(s) 81, 606
BslI CCNNNNNNNGG 5 cut(s) 247, 505, 893, 899, 1161
BsmAI GTCTC 4 cut(s) 477, 658, 780, 930
BsmFI GGGAC 2 cut(s) 81, 606
BsnI GGCC 4 cut(s) 86, 300, 615, 1026
Bso31I GGTCTC 1 cut(s) 658
BsoBI CYCGRG 3 cut(s) 34, 277, 616
Bsp120I GGGCCC 1 cut(s) 613
Bsp1286I GDGCHC 2 cut(s) 312, 617
Bsp143I GATC 8 cut(s) 78, 269, 289, 405, 608, 634, 952, 1141
Bsp1720I GCTNAGC 1 cut(s) 311
Bsp19I CCATGG 2 cut(s) 504, 970
BspANI GGCC 4 cut(s) 86, 300, 615, 1026
BspCNI CTCAG 6 cut(s) 258, 324, 415, 790, 877, 1140
BspFNI CGCG 3 cut(s) 69, 71, 1193
BspLI GGNNCC 7 cut(s) 96, 97, 615, 621, 622, 636, 916
BspMI ACCTGC 1 cut(s) 558
BspPI GGATC 4 cut(s) 264, 400, 629, 642
BspQI GCTCTTC 1 cut(s) 1306
BspTNI GGTCTC 1 cut(s) 658
BsrBI CCGCTC 2 cut(s) 39, 232
BsrDI GCAATG 1 cut(s) 1141
BsrI ACTGG 1 cut(s) 1097
BssECI CCNNGG 5 cut(s) 504, 616, 970, 1014, 1049
BssMI GATC 8 cut(s) 78, 269, 289, 405, 608, 634, 952, 1141
BssT1I CCWWGG 3 cut(s) 504, 970, 1049
Bst4CI ACNGT 2 cut(s) 774, 1172
Bst6I CTCTTC 3 cut(s) 651, 823, 1306
BstDEI CTNAG 6 cut(s) 266, 311, 402, 777, 864, 1148
BstDSI CCRYGG 2 cut(s) 504, 970
BstF5I GGATG 3 cut(s) 166, 410, 1024
BstFNI CGCG 3 cut(s) 69, 71, 1193
BstHHI GCGC 1 cut(s) 71
BstKTI GATC 8 cut(s) 81, 272, 292, 408, 611, 637, 955, 1144
BstMAI GTCTC 4 cut(s) 477, 658, 780, 930
BstMBI GATC 8 cut(s) 78, 269, 289, 405, 608, 634, 952, 1141
BstMWI GCNNNNNNNGC 5 cut(s) 680, 865, 982, 1039, 1051
BstNSI RCATGY 1 cut(s) 805
BstSCI CCNGG 3 cut(s) 615, 616, 892
BstSFI CTRYAG 1 cut(s) 1260
BstSLI GKGCMC 1 cut(s) 617
BstUI CGCG 3 cut(s) 69, 71, 1193
BstV1I GCAGC 5 cut(s) 871, 997, 1051, 1054, 1246
BstV2I GAAGAC 1 cut(s) 579
BstX2I RGATCY 3 cut(s) 269, 405, 634
BstXI CCANNNNNNTGG 1 cut(s) 631
BstYI RGATCY 3 cut(s) 269, 405, 634
BsuRI GGCC 4 cut(s) 86, 300, 615, 1026
BtgI CCRYGG 2 cut(s) 504, 970
BtsCI GGATG 3 cut(s) 166, 410, 1024
BveI ACCTGC 1 cut(s) 558
CfoI GCGC 1 cut(s) 71
Cfr13I GGNCC 6 cut(s) 95, 255, 613, 614, 620, 1152
Cfr9I CCCGGG 1 cut(s) 616
CseI GACGC 1 cut(s) 271
Csp6I GTAC 3 cut(s) 58, 1029, 1254
CviAII CATG 8 cut(s) 103, 173, 247, 505, 793, 802, 971, 1290
CviQI GTAC 3 cut(s) 58, 1029, 1254
DdeI CTNAG 6 cut(s) 266, 311, 402, 777, 864, 1148
DpnI GATC 8 cut(s) 80, 271, 291, 407, 610, 636, 954, 1143
DpnII GATC 8 cut(s) 78, 269, 289, 405, 608, 634, 952, 1141
DriI GACNNNNNGTC 2 cut(s) 52, 333
EaeI YGGCCR 2 cut(s) 298, 1024
Eam1104I CTCTTC 3 cut(s) 651, 823, 1306
Eam1105I GACNNNNNGTC 2 cut(s) 52, 333
EarI CTCTTC 3 cut(s) 651, 823, 1306
EciI GGCGGA 1 cut(s) 393
Ecl136II GAGCTC 1 cut(s) 310
Eco130I CCWWGG 3 cut(s) 504, 970, 1049
Eco24I GRGCYC 2 cut(s) 312, 617
Eco31I GGTCTC 1 cut(s) 658
Eco47I GGWCC 4 cut(s) 95, 255, 620, 1152
Eco53kI GAGCTC 1 cut(s) 310
Eco57I CTGAAG 3 cut(s) 573, 1019, 1287
Eco88I CYCGRG 3 cut(s) 34, 277, 616
EcoICRI GAGCTC 1 cut(s) 310
EcoO109I RGGNCCY 2 cut(s) 95, 620
EcoT14I CCWWGG 3 cut(s) 504, 970, 1049
EcoT38I GRGCYC 2 cut(s) 312, 617
ErhI CCWWGG 3 cut(s) 504, 970, 1049
FaeI CATG 8 cut(s) 106, 176, 250, 508, 796, 805, 974, 1293
FaqI GGGAC 2 cut(s) 81, 606
FatI CATG 8 cut(s) 102, 172, 246, 504, 792, 801, 970, 1289
FauI CCCGC 1 cut(s) 237
FauNDI CATATG 1 cut(s) 630
FokI GGATG 3 cut(s) 153, 397, 1031
FriOI GRGCYC 2 cut(s) 312, 617
FspBI CTAG 5 cut(s) 341, 561, 726, 1050, 1266
GlaI GCGC 1 cut(s) 70
GsaI CCCAGC 2 cut(s) 95, 1230
GsuI CTGGAG 1 cut(s) 1259
HaeIII GGCC 4 cut(s) 86, 300, 615, 1026
HapII CCGG 3 cut(s) 9, 617, 894
HgaI GACGC 1 cut(s) 271
HhaI GCGC 1 cut(s) 71
Hin1II CATG 8 cut(s) 106, 176, 250, 508, 796, 805, 974, 1293
Hin6I GCGC 1 cut(s) 69
HinP1I GCGC 1 cut(s) 69
HincII GTYRAC 2 cut(s) 338, 1099
HindII GTYRAC 2 cut(s) 338, 1099
HinfI GANTC 8 cut(s) 5, 53, 62, 74, 206, 366, 781, 1286
HpaII CCGG 3 cut(s) 9, 617, 894
Hpy166II GTNNAC 2 cut(s) 338, 1099
Hpy188III TCNNGA 1 cut(s) 512
Hpy8I GTNNAC 2 cut(s) 338, 1099
Hpy99I CGWCG 7 cut(s) 50, 53, 455, 1009, 1012, 1084, 1210
HpyAV CCTTC 4 cut(s) 648, 660, 705, 1268
HpyCH4III ACNGT 2 cut(s) 774, 1172
HpyCH4IV ACGT 1 cut(s) 450
HpyCH4V TGCA 5 cut(s) 28, 533, 805, 985, 1234
HpyF10VI GCNNNNNNNGC 5 cut(s) 680, 865, 982, 1039, 1051
HpyF3I CTNAG 6 cut(s) 266, 311, 402, 777, 864, 1148
HpySE526I ACGT 1 cut(s) 450
Hsp92II CATG 8 cut(s) 106, 176, 250, 508, 796, 805, 974, 1293
HspAI GCGC 1 cut(s) 69
KflI GGGWCCC 2 cut(s) 95, 620
Kzo9I GATC 8 cut(s) 78, 269, 289, 405, 608, 634, 952, 1141
LguI GCTCTTC 1 cut(s) 1306
LmnI GCTCC 4 cut(s) 237, 427, 688, 920
Lsp1109I GCAGC 5 cut(s) 871, 997, 1051, 1054, 1246
MaeI CTAG 5 cut(s) 341, 561, 726, 1050, 1266
MaeII ACGT 1 cut(s) 450
MaeIII GTNAC 2 cut(s) 11, 717
MalI GATC 8 cut(s) 80, 271, 291, 407, 610, 636, 954, 1143
MbiI CCGCTC 2 cut(s) 39, 232
MboI GATC 8 cut(s) 78, 269, 289, 405, 608, 634, 952, 1141
MflI RGATCY 3 cut(s) 269, 405, 634
MhlI GDGCHC 2 cut(s) 312, 617
MluCI AATT 5 cut(s) 515, 730, 832, 871, 1165
MlyI GAGTC 5 cut(s) 56, 62, 83, 375, 790
MmeI TCCRAC 3 cut(s) 219, 297, 552
MseI TTAA 1 cut(s) 1309
MslI CAYNNNNRTG 1 cut(s) 171
MspA1I CMGCKG 2 cut(s) 18, 1237
MspI CCGG 3 cut(s) 9, 617, 894
MspR9I CCNGG 3 cut(s) 617, 618, 894
MvnI CGCG 3 cut(s) 69, 71, 1193
MwoI GCNNNNNNNGC 5 cut(s) 680, 865, 982, 1039, 1051
NciI CCSGG 3 cut(s) 617, 618, 894
NcoI CCATGG 2 cut(s) 504, 970
NdeI CATATG 1 cut(s) 630
NdeII GATC 8 cut(s) 78, 269, 289, 405, 608, 634, 952, 1141
NlaIII CATG 8 cut(s) 106, 176, 250, 508, 796, 805, 974, 1293
NlaIV GGNNCC 7 cut(s) 96, 97, 615, 621, 622, 636, 916
NmeAIII GCCGAG 1 cut(s) 62
NspI RCATGY 1 cut(s) 805
PaeR7I CTCGAG 1 cut(s) 34
PciSI GCTCTTC 1 cut(s) 1306
PcsI WCGNNNNNNNCGW 4 cut(s) 57, 422, 456, 1079
PfeI GAWTC 3 cut(s) 5, 206, 1286
Pfl23II CGTACG 2 cut(s) 57, 1028
PleI GAGTC 5 cut(s) 56, 61, 82, 374, 789
PpsI GAGTC 5 cut(s) 56, 61, 82, 374, 789
PpuMI RGGWCCY 2 cut(s) 95, 620
Psp124BI GAGCTC 1 cut(s) 312
Psp5II RGGWCCY 2 cut(s) 95, 620
PspFI CCCAGC 2 cut(s) 91, 1226
PspLI CGTACG 2 cut(s) 57, 1028
PspN4I GGNNCC 7 cut(s) 96, 97, 615, 621, 622, 636, 916
PspOMI GGGCCC 1 cut(s) 613
PspPI GGNCC 6 cut(s) 95, 255, 613, 614, 620, 1152
PspPPI RGGWCCY 2 cut(s) 95, 620
PspXI VCTCGAGB 1 cut(s) 34
PsuI RGATCY 3 cut(s) 269, 405, 634
PvuII CAGCTG 1 cut(s) 1237
RsaI GTAC 3 cut(s) 59, 1030, 1255
RsaNI GTAC 3 cut(s) 58, 1029, 1254
RseI CAYNNNNRTG 1 cut(s) 171
SacI GAGCTC 1 cut(s) 312
SapI GCTCTTC 1 cut(s) 1306
SaqAI TTAA 1 cut(s) 1309
Sau3AI GATC 8 cut(s) 78, 269, 289, 405, 608, 634, 952, 1141
Sau96I GGNCC 6 cut(s) 95, 255, 613, 614, 620, 1152
ScaI AGTACT 1 cut(s) 1255
SchI GAGTC 5 cut(s) 56, 62, 83, 375, 790
ScrFI CCNGG 3 cut(s) 617, 618, 894
SduI GDGCHC 2 cut(s) 312, 617
SfcI CTRYAG 1 cut(s) 1260
Sfr274I CTCGAG 1 cut(s) 34
SinI GGWCC 4 cut(s) 95, 255, 620, 1152
SlaI CTCGAG 1 cut(s) 34
SmaI CCCGGG 1 cut(s) 618
SmiMI CAYNNNNRTG 1 cut(s) 171
SmlI CTYRAG 2 cut(s) 34, 927
SmoI CTYRAG 2 cut(s) 34, 927
Sse9I AATT 5 cut(s) 515, 730, 832, 871, 1165
SspI AATATT 2 cut(s) 751, 907
SspMI CTAG 5 cut(s) 341, 561, 726, 1050, 1266
SstI GAGCTC 1 cut(s) 312
StyD4I CCNGG 3 cut(s) 615, 616, 892
StyI CCWWGG 3 cut(s) 504, 970, 1049
TaaI ACNGT 2 cut(s) 774, 1172
TaiI ACGT 1 cut(s) 453
TasI AATT 5 cut(s) 515, 730, 832, 871, 1165
TatI WGTACW 1 cut(s) 1253
TauI GCSGC 5 cut(s) 42, 45, 300, 459, 1196
TfiI GAWTC 3 cut(s) 5, 206, 1286
Tru1I TTAA 1 cut(s) 1309
Tru9I TTAA 1 cut(s) 1309
TseI GCWGC 5 cut(s) 859, 985, 1039, 1042, 1234
TspDTI ATGAA 4 cut(s) 585, 809, 810, 1103
TspGWI ACGGA 1 cut(s) 1144
TspMI CCCGGG 1 cut(s) 616
VpaK11BI GGWCC 4 cut(s) 95, 255, 620, 1152
XapI RAATTY 3 cut(s) 515, 832, 871
XceI RCATGY 1 cut(s) 805
XhoI CTCGAG 1 cut(s) 34
XmaI CCCGGG 1 cut(s) 616
XmaJI CCTAGG 1 cut(s) 1049
XspI CTAG 5 cut(s) 341, 561, 726, 1050, 1266
ZrmI AGTACT 1 cut(s) 1255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.