FvH4_1g00220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012415
Physical Location & Seq
Forward (+)
117654 .. 119781
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g00220.t1

Sequence Viewer

Length: 1731 bp
ATGAACCCATCGTTGTCGTCAAAGGCCGGGAAGAAGCCCTCTCCGGCGCCGGCGAATAAGAAGAAGAAGAAAAAGGAGCATTGCACTCGGAACCCGTTACAGGATCTCAATGCCATCAGCACCACTAGCAACCACGGCAGCGAGGCTTCGTCTTCATCTTCAATCTCCATCGAAGCCCCACGAGGTTGCCTCAGGTTCTTTCTCTCTCATTCATCTTCTTCTAATTTAAAAACCCCTATACGCCCCAGGCCTAGAAATCTCTCCAAAACCCATGTTTCCAGAAACCCAGAGAAACCAAAACTCAAGACTAGTTCTTCTTGCTTGTATCACATGCCTAGTTCTAGGAATGTCCAAAAGTTGAAAGCTTGTCCAGCTTTGAACTCCAAAGGTTGTTCCTTTGAACTCAAAGGTCAGTCCAATGAGCTTAAATCAGGTGCTGGTGATGGAACTCTTACACCCTCATGTAAGAAACCTACTGGGTTAGGTTTGGGATGCAAAGTCGACAGGAATGTGGAAGAAAATTCTAACAAAAGTGATAGTACAACTCCTCCTGTTCAGGCCTCTGTGTCTCCAGAGATACAACTTGGATCGTCTGCCGTGTCATCAGCCTCGCTGGCTTGTTATGCTGCCGGCCATGTGCTGTCGGGGATCACTGACAAGAGGAAGTGCAGGCCTAGGGGAATTCTCAATGTAGGAGAGAATGATTCAGGCTTTGGGAAAGGGAAGGGTTTGTGCAGTTTTGAGGAAGTGGTGGAGGATGAGGATGGATCTGCCCAAGGAATGGTTGGAGATCCTGATGCTTCTTTGGTGCCCTTACCTACTGAAGCTTCAATGCATTGGCTTTTGTCACCATGTAATGAGGAAGATGAGGATCACAAGGAGCATTCTGAGACTAGTTTTCAGAATCCTGATGTGTCCCCCAATAACCACTTGGAAAGTAGTACTTGCCGTAGCAGGAGGAACACTTCCATTTCTCCAGGTGGAGTTCCTAAATTTCAAGAATTGTTAGAGCCCTTGAATGAGCATGTGCCTGTTTTGTCTTCTCCCCTTTGTAGACCTGGTTGTGAGGCTCTACCCTTGAAAGATGAAAGACAATGCACTTATGATTTTGATGTCGGGAATTCTCCGTTTTCTATGGCTTCAATGAGTAGTGGCAATGTTATTCTTACACCTCAATCAGACTCGAGTGCAGATGGACATGCTGGCCTGTCACGGTCTAATACAGACAACCGTAGGAAACACTATGAGTCTGAACTCAATTCAGTGGCTGAAGCTATTCGGATGGCGAGCTTGTCTCCAAACCGTCAGGAACTGTTGGATGATCAGGCTTCCTGGCATCCCAACTCCACGTTAGAGTATGTGTCACAATCCCAGCTGAGGATATCATGGAGAGAAGGTTTGATGAGTCGTATGTATGACATGGATGAAGATGATTGCTGCAGATGTTTATCAGACGAAGAGGAAGATATCAATGGCTGCAGCAATGACCTTTCCATAGAATCCTGTCAAAGCCCTGAAATCACTAATAATTTTAGGGGAGATCAGATTTTTACCGATACTTCCTGGTCTGCTGATATACTGGATGATGAACCAGGAGTTACTGCAAAAGTTATAGAAGACATTCCTCCTCAATCATGTGCCGAATCCATAAGTACTGATGGAGGCGGCCTGCTTGCTTCAAGGGATTCAGATTGGAATCTCTGCTACAAGAATGAGCTCTTCCATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

577

Amino Acids

62.43

Weight (kDa)

5.38

Isoelectric Point (pI)

66.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012415)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43990
fragaria_vesca FvH4_1g00220
malus_domestica MD00G1086900.v1.1 MD15G1142800.v1.1
prunus_persica Prupe.7G270200_v2.0.a1 Prupe.7G270200_v2.0.a1
pyrus_communis pycom15g12890 pycom290g00170
rosa_chinensis RchiOBHm_Chr2g0084511
rosa_laevigata RLG00000015623
rosa_multiflora Rmu_co8107158.1_g000001 Rmu_sc0009647.1_g000004
rosa_roxburghii Rroxscaffold_2G00156000
rosa_rugosa Rorug01G0454600
rosa_samantha Rh2AG002700 Rh2BG002300 Rh2CG002800 Rh2DG002800
rosa_wichuraiana Rw2G000240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 46, 808
AccB7I CCANNNNNTGG 1 cut(s) 781
AccI GTMKAC 2 cut(s) 501, 1054
AciI CCGC 1 cut(s) 1665
AclWI GGATC 6 cut(s) 111, 595, 656, 775, 785, 879
AcoI YGGCCR 1 cut(s) 631
AcsI RAATTY 4 cut(s) 520, 681, 992, 1120
AcuI CTGAAG 2 cut(s) 843, 1290
AcyI GRCGYC 1 cut(s) 47
AfaI GTAC 3 cut(s) 541, 943, 1654
AfiI CCNNNNNNNGG 3 cut(s) 100, 781, 1377
AhlI ACTAGT 2 cut(s) 308, 893
AjnI CCWGG 6 cut(s) 245, 976, 1057, 1331, 1562, 1591
AluBI AGCT 8 cut(s) 365, 374, 424, 827, 1274, 1290, 1375, 1717
AluI AGCT 8 cut(s) 365, 374, 424, 827, 1274, 1290, 1375, 1717
Alw21I GWGCWC 1 cut(s) 1719
Alw26I GTCTC 3 cut(s) 573, 884, 1299
AlwI GGATC 6 cut(s) 111, 595, 656, 775, 785, 879
AlwNI CAGNNNCTG 3 cut(s) 437, 1268, 1312
Ama87I CYCGRG 1 cut(s) 1183
AoxI GGCC 7 cut(s) 24, 248, 558, 631, 671, 1204, 1666
ApeKI GCWGC 5 cut(s) 138, 626, 1437, 1476, 1479
ApoI RAATTY 4 cut(s) 520, 681, 992, 1120
Asp700I GAANNNNTTC 2 cut(s) 1275, 1620
AspA2I CCTAGG 1 cut(s) 674
AspLEI GCGC 1 cut(s) 49
AsuC2I CCSGG 1 cut(s) 28
AsuHPI GGTGA 2 cut(s) 452, 840
AvaI CYCGRG 1 cut(s) 1183
AvrII CCTAGG 1 cut(s) 674
AxyI CCTNAGG 1 cut(s) 191
BaeGI GKGCMC 1 cut(s) 813
BanI GGYRCC 2 cut(s) 46, 808
BanII GRGCYC 2 cut(s) 1014, 1719
BauI CACGAG 1 cut(s) 180
BbsI GAAGAC 3 cut(s) 144, 1032, 1623
Bbv12I GWGCWC 1 cut(s) 1719
BbvCI CCTCAGC 1 cut(s) 1376
BbvI GCAGC 5 cut(s) 150, 613, 1424, 1463, 1491
BccI CCATC 8 cut(s) 16, 122, 176, 437, 758, 1187, 1276, 1652
BceAI ACGGC 3 cut(s) 151, 581, 933
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 6 cut(s) 247, 978, 1059, 1333, 1564, 1593
BclI TGATCA 1 cut(s) 1321
BcnI CCSGG 1 cut(s) 28
BcoDI GTCTC 3 cut(s) 573, 884, 1299
BcuI ACTAGT 2 cut(s) 308, 893
BfaI CTAG 7 cut(s) 126, 252, 309, 336, 342, 675, 894
BfmI CTRYAG 2 cut(s) 1438, 1477
BfoI RGCGCY 1 cut(s) 50
BglI GCCNNNNNGGC 1 cut(s) 614
BisI GCNGC 6 cut(s) 139, 627, 1438, 1477, 1480, 1666
BlnI CCTAGG 1 cut(s) 674
BlsI GCNGC 6 cut(s) 140, 628, 1439, 1478, 1481, 1667
BmcAI AGTACT 2 cut(s) 943, 1654
Bme1390I CCNGG 7 cut(s) 28, 247, 978, 1059, 1333, 1564, 1593
BmeT110I CYCGRG 1 cut(s) 1183
BmiI GGNNCC 3 cut(s) 48, 92, 810
BmrFI CCNGG 7 cut(s) 28, 247, 978, 1059, 1333, 1564, 1593
BmrI ACTGGG 1 cut(s) 486
BmsI GCATC 3 cut(s) 482, 787, 1345
BmuI ACTGGG 1 cut(s) 486
BpiI GAAGAC 3 cut(s) 144, 1032, 1623
BplI GAGNNNNNCTC 4 cut(s) 174, 206, 1279, 1311
BpmI CTGGAG 2 cut(s) 555, 960
Bpu10I CCTNAGC 1 cut(s) 1376
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 28
BsaBI GATNNNNATC 3 cut(s) 870, 1447, 1695
BsaHI GRCGYC 1 cut(s) 47
BsaJI CCNNGG 4 cut(s) 133, 245, 674, 775
BsaXI ACNNNNNCTCC 4 cut(s) 532, 562, 1381, 1411
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 781, 1377
Bse118I RCCGGY 2 cut(s) 49, 629
Bse1I ACTGG 2 cut(s) 481, 1584
Bse21I CCTNAGG 1 cut(s) 191
Bse3DI GCAATG 3 cut(s) 79, 1162, 1489
Bse8I GATNNNNATC 3 cut(s) 870, 1447, 1695
BseBI CCWGG 6 cut(s) 247, 978, 1059, 1333, 1564, 1593
BseDI CCNNGG 4 cut(s) 133, 245, 674, 775
BseGI GGATG 8 cut(s) 497, 763, 769, 1287, 1324, 1336, 1429, 1588
BseJI GATNNNNATC 3 cut(s) 870, 1447, 1695
BseLI CCNNNNNNNGG 3 cut(s) 100, 781, 1377
BseMI GCAATG 3 cut(s) 79, 1162, 1489
BseMII CTCAG 3 cut(s) 205, 879, 1367
BseNI ACTGG 2 cut(s) 481, 1584
BseRI GAGGAG 2 cut(s) 537, 1617
BseSI GKGCMC 1 cut(s) 813
BseXI GCAGC 5 cut(s) 150, 613, 1424, 1463, 1491
BseYI CCCAGC 1 cut(s) 1371
BsgI GTGCAG 3 cut(s) 688, 754, 1209
BshFI GGCC 7 cut(s) 26, 250, 560, 633, 673, 1206, 1668
BshNI GGYRCC 2 cut(s) 46, 808
BsiHKAI GWGCWC 1 cut(s) 1719
BsiHKCI CYCGRG 1 cut(s) 1183
BsiSI CCGG 4 cut(s) 27, 44, 50, 630
BslFI GGGAC 1 cut(s) 901
BslI CCNNNNNNNGG 3 cut(s) 100, 781, 1377
BsmAI GTCTC 3 cut(s) 573, 884, 1299
BsmFI GGGAC 1 cut(s) 901
BsmI GAATGC 1 cut(s) 883
BsnI GGCC 7 cut(s) 26, 250, 560, 633, 673, 1206, 1668
BsoBI CYCGRG 1 cut(s) 1183
Bsp1286I GDGCHC 3 cut(s) 813, 1014, 1719
Bsp143I GATC 8 cut(s) 103, 587, 648, 767, 790, 871, 1321, 1540
BspACI CCGC 1 cut(s) 1665
BspANI GGCC 7 cut(s) 26, 250, 560, 633, 673, 1206, 1668
BspCNI CTCAG 3 cut(s) 204, 880, 1368
BspLI GGNNCC 3 cut(s) 48, 92, 810
BspMAI CTGCAG 2 cut(s) 1442, 1481
BspPI GGATC 6 cut(s) 111, 595, 656, 775, 785, 879
BspQI GCTCTTC 1 cut(s) 1724
BspT107I GGYRCC 2 cut(s) 46, 808
BsrDI GCAATG 3 cut(s) 79, 1162, 1489
BsrFI RCCGGY 2 cut(s) 49, 629
BsrI ACTGG 2 cut(s) 481, 1584
BssAI RCCGGY 2 cut(s) 49, 629
BssECI CCNNGG 4 cut(s) 133, 245, 674, 775
BssMI GATC 8 cut(s) 103, 587, 648, 767, 790, 871, 1321, 1540
BssNI GRCGYC 1 cut(s) 47
BssSI CACGAG 1 cut(s) 180
BssT1I CCWWGG 2 cut(s) 674, 775
Bst2BI CACGAG 1 cut(s) 180
Bst2UI CCWGG 6 cut(s) 247, 978, 1059, 1333, 1564, 1593
Bst4CI ACNGT 4 cut(s) 1215, 1232, 1304, 1314
Bst6I CTCTTC 2 cut(s) 1452, 1724
BstACI GRCGYC 1 cut(s) 47
BstC8I GCNNGC 8 cut(s) 51, 615, 631, 671, 1204, 1288, 1670, 1674
BstDEI CTNAG 3 cut(s) 191, 888, 1376
BstDSI CCRYGG 1 cut(s) 133
BstF5I GGATG 8 cut(s) 497, 763, 769, 1287, 1324, 1336, 1429, 1588
BstH2I RGCGCY 1 cut(s) 50
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 8 cut(s) 106, 590, 651, 770, 793, 874, 1324, 1543
BstMAI GTCTC 3 cut(s) 573, 884, 1299
BstMBI GATC 8 cut(s) 103, 587, 648, 767, 790, 871, 1321, 1540
BstMWI GCNNNNNNNGC 5 cut(s) 126, 135, 371, 614, 623
BstNI CCWGG 6 cut(s) 247, 978, 1059, 1333, 1564, 1593
BstNSI RCATGY 3 cut(s) 334, 1028, 1202
BstSCI CCNGG 7 cut(s) 26, 245, 976, 1057, 1331, 1562, 1591
BstSFI CTRYAG 2 cut(s) 1438, 1477
BstSLI GKGCMC 1 cut(s) 813
BstV1I GCAGC 5 cut(s) 150, 613, 1424, 1463, 1491
BstV2I GAAGAC 3 cut(s) 144, 1032, 1623
BstX2I RGATCY 3 cut(s) 103, 767, 790
BstYI RGATCY 3 cut(s) 103, 767, 790
Bsu36I CCTNAGG 1 cut(s) 191
BsuRI GGCC 7 cut(s) 26, 250, 560, 633, 673, 1206, 1668
BtgI CCRYGG 1 cut(s) 133
BtsCI GGATG 8 cut(s) 497, 763, 769, 1287, 1324, 1336, 1429, 1588
BtsIMutI CAGTG 2 cut(s) 651, 1269
Cac8I GCNNGC 8 cut(s) 51, 615, 631, 671, 1204, 1288, 1670, 1674
CaiI CAGNNNCTG 3 cut(s) 437, 1268, 1312
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 2 cut(s) 49, 629
CsiI ACCWGGT 1 cut(s) 1057
Csp6I GTAC 3 cut(s) 540, 942, 1653
CviQI GTAC 3 cut(s) 540, 942, 1653
DdeI CTNAG 3 cut(s) 191, 888, 1376
DinI GGCGCC 1 cut(s) 48
DpnI GATC 8 cut(s) 105, 589, 650, 769, 792, 873, 1323, 1542
DpnII GATC 8 cut(s) 103, 587, 648, 767, 790, 871, 1321, 1540
DraI TTTAAA 1 cut(s) 228
EaeI YGGCCR 1 cut(s) 631
Eam1104I CTCTTC 2 cut(s) 1452, 1724
EarI CTCTTC 2 cut(s) 1452, 1724
Ecl136II GAGCTC 1 cut(s) 1717
Eco130I CCWWGG 2 cut(s) 674, 775
Eco147I AGGCCT 3 cut(s) 250, 560, 673
Eco24I GRGCYC 2 cut(s) 1014, 1719
Eco32I GATATC 2 cut(s) 1383, 1468
Eco53kI GAGCTC 1 cut(s) 1717
Eco57I CTGAAG 2 cut(s) 843, 1290
Eco81I CCTNAGG 1 cut(s) 191
Eco88I CYCGRG 1 cut(s) 1183
EcoICRI GAGCTC 1 cut(s) 1717
EcoRI GAATTC 2 cut(s) 681, 1120
EcoRII CCWGG 6 cut(s) 245, 976, 1057, 1331, 1562, 1591
EcoRV GATATC 2 cut(s) 1383, 1468
EcoT14I CCWWGG 2 cut(s) 674, 775
EcoT22I ATGCAT 1 cut(s) 837
EcoT38I GRGCYC 2 cut(s) 1014, 1719
EgeI GGCGCC 1 cut(s) 48
EheI GGCGCC 1 cut(s) 48
ErhI CCWWGG 2 cut(s) 674, 775
FalI AAGNNNNNCTT 2 cut(s) 928, 960
FaqI GGGAC 1 cut(s) 901
FbaI TGATCA 1 cut(s) 1321
FblI GTMKAC 2 cut(s) 501, 1054
Fnu4HI GCNGC 6 cut(s) 139, 627, 1438, 1477, 1480, 1666
FokI GGATG 8 cut(s) 504, 770, 776, 1294, 1323, 1331, 1436, 1595
FriOI GRGCYC 2 cut(s) 1014, 1719
Fsp4HI GCNGC 6 cut(s) 139, 627, 1438, 1477, 1480, 1666
FspBI CTAG 7 cut(s) 126, 252, 309, 336, 342, 675, 894
GlaI GCGC 1 cut(s) 48
GluI GCNGC 6 cut(s) 139, 627, 1438, 1477, 1480, 1666
GsaI CCCAGC 1 cut(s) 1375
GsuI CTGGAG 2 cut(s) 555, 960
HaeII RGCGCY 1 cut(s) 50
HaeIII GGCC 7 cut(s) 26, 250, 560, 633, 673, 1206, 1668
HapII CCGG 4 cut(s) 27, 44, 50, 630
HhaI GCGC 1 cut(s) 49
Hin1I GRCGYC 1 cut(s) 47
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HindIII AAGCTT 2 cut(s) 363, 825
HinfI GANTC 9 cut(s) 704, 904, 1181, 1247, 1405, 1499, 1643, 1685, 1696
HpaII CCGG 4 cut(s) 27, 44, 50, 630
HphI GGTGA 2 cut(s) 452, 840
Hpy166II GTNNAC 2 cut(s) 502, 1055
Hpy188I TCNGA 9 cut(s) 90, 889, 903, 1180, 1252, 1281, 1453, 1545, 1690
Hpy188III TCNNGA 8 cut(s) 279, 304, 572, 794, 908, 998, 1117, 1307
Hpy8I GTNNAC 2 cut(s) 502, 1055
HpyAV CCTTC 2 cut(s) 718, 1388
HpyCH4III ACNGT 4 cut(s) 1215, 1232, 1304, 1314
HpyCH4IV ACGT 1 cut(s) 1349
HpyF10VI GCNNNNNNNGC 5 cut(s) 126, 135, 371, 614, 623
HpyF3I CTNAG 3 cut(s) 191, 888, 1376
HpySE526I ACGT 1 cut(s) 1349
Hsp92I GRCGYC 1 cut(s) 47
HspAI GCGC 1 cut(s) 47
KasI GGCGCC 1 cut(s) 46
KroI GCCGGC 2 cut(s) 49, 629
KroNI GCCGGC 2 cut(s) 51, 631
Ksp22I TGATCA 1 cut(s) 1321
Kzo9I GATC 8 cut(s) 103, 587, 648, 767, 790, 871, 1321, 1540
LguI GCTCTTC 1 cut(s) 1724
LmnI GCTCC 2 cut(s) 76, 880
Lsp1109I GCAGC 5 cut(s) 150, 613, 1424, 1463, 1491
LweI GCATC 3 cut(s) 482, 787, 1345
MabI ACCWGGT 1 cut(s) 1057
MaeI CTAG 7 cut(s) 126, 252, 309, 336, 342, 675, 894
MaeII ACGT 1 cut(s) 1349
MaeIII GTNAC 5 cut(s) 96, 846, 1209, 1362, 1597
MalI GATC 8 cut(s) 105, 589, 650, 769, 792, 873, 1323, 1542
MboI GATC 8 cut(s) 103, 587, 648, 767, 790, 871, 1321, 1540
MflI RGATCY 3 cut(s) 103, 767, 790
MhlI GDGCHC 3 cut(s) 813, 1014, 1719
MluCI AATT 8 cut(s) 223, 520, 681, 992, 1001, 1120, 1258, 1528
Mly113I GGCGCC 1 cut(s) 47
MlyI GAGTC 3 cut(s) 1175, 1256, 1414
MmeI TCCRAC 2 cut(s) 766, 1296
Mph1103I ATGCAT 1 cut(s) 837
MreI CGCCGGCG 1 cut(s) 49
MroNI GCCGGC 2 cut(s) 49, 629
MroXI GAANNNNTTC 2 cut(s) 1275, 1620
MseI TTAA 3 cut(s) 227, 426, 1729
MslI CAYNNNNRTG 1 cut(s) 460
MspA1I CMGCKG 1 cut(s) 1375
MspI CCGG 4 cut(s) 27, 44, 50, 630
MspR9I CCNGG 7 cut(s) 28, 247, 978, 1059, 1333, 1564, 1593
Mva1269I GAATGC 1 cut(s) 883
MvaI CCWGG 6 cut(s) 247, 978, 1059, 1333, 1564, 1593
MwoI GCNNNNNNNGC 5 cut(s) 126, 135, 371, 614, 623
NaeI GCCGGC 2 cut(s) 51, 631
NarI GGCGCC 1 cut(s) 47
NciI CCSGG 1 cut(s) 28
NdeII GATC 8 cut(s) 103, 587, 648, 767, 790, 871, 1321, 1540
NgoMIV GCCGGC 2 cut(s) 49, 629
NlaIV GGNNCC 3 cut(s) 48, 92, 810
NmuCI GTSAC 3 cut(s) 846, 1209, 1362
NsiI ATGCAT 1 cut(s) 837
NspI RCATGY 3 cut(s) 334, 1028, 1202
PaeR7I CTCGAG 1 cut(s) 1183
PceI AGGCCT 3 cut(s) 250, 560, 673
PciSI GCTCTTC 1 cut(s) 1724
PctI GAATGC 1 cut(s) 883
PdiI GCCGGC 2 cut(s) 51, 631
PdmI GAANNNNTTC 2 cut(s) 1275, 1620
PfeI GAWTC 6 cut(s) 704, 904, 1499, 1643, 1685, 1696
PflMI CCANNNNNTGG 1 cut(s) 781
PkrI GCNGC 6 cut(s) 140, 628, 1439, 1478, 1481, 1667
PleI GAGTC 3 cut(s) 1175, 1255, 1413
PluTI GGCGCC 1 cut(s) 50
PpsI GAGTC 3 cut(s) 1175, 1255, 1413
Psp124BI GAGCTC 1 cut(s) 1719
Psp6I CCWGG 6 cut(s) 245, 976, 1057, 1331, 1562, 1591
PspFI CCCAGC 1 cut(s) 1371
PspGI CCWGG 6 cut(s) 245, 976, 1057, 1331, 1562, 1591
PspN4I GGNNCC 3 cut(s) 48, 92, 810
PspXI VCTCGAGB 1 cut(s) 1183
PstI CTGCAG 2 cut(s) 1442, 1481
PstNI CAGNNNCTG 3 cut(s) 437, 1268, 1312
PsuI RGATCY 3 cut(s) 103, 767, 790
PvuII CAGCTG 1 cut(s) 1375
RsaI GTAC 3 cut(s) 541, 943, 1654
RsaNI GTAC 3 cut(s) 540, 942, 1653
RseI CAYNNNNRTG 1 cut(s) 460
SacI GAGCTC 1 cut(s) 1719
SalI GTCGAC 1 cut(s) 500
SapI GCTCTTC 1 cut(s) 1724
SaqAI TTAA 3 cut(s) 227, 426, 1729
SatI GCNGC 6 cut(s) 139, 627, 1438, 1477, 1480, 1666
Sau3AI GATC 8 cut(s) 103, 587, 648, 767, 790, 871, 1321, 1540
ScaI AGTACT 2 cut(s) 943, 1654
SchI GAGTC 3 cut(s) 1175, 1256, 1414
ScrFI CCNGG 7 cut(s) 28, 247, 978, 1059, 1333, 1564, 1593
SduI GDGCHC 3 cut(s) 813, 1014, 1719
SexAI ACCWGGT 1 cut(s) 1057
SfaNI GCATC 3 cut(s) 482, 787, 1345
SfcI CTRYAG 2 cut(s) 1438, 1477
SfoI GGCGCC 1 cut(s) 48
Sfr274I CTCGAG 1 cut(s) 1183
SgrAI CRCCGGYG 1 cut(s) 49
SlaI CTCGAG 1 cut(s) 1183
SmiMI CAYNNNNRTG 1 cut(s) 460
SmlI CTYRAG 2 cut(s) 302, 1183
SmoI CTYRAG 2 cut(s) 302, 1183
SpeI ACTAGT 2 cut(s) 308, 893
Sse9I AATT 8 cut(s) 223, 520, 681, 992, 1001, 1120, 1258, 1528
SseBI AGGCCT 3 cut(s) 250, 560, 673
SsiI CCGC 1 cut(s) 1665
SspDI GGCGCC 1 cut(s) 46
SspMI CTAG 7 cut(s) 126, 252, 309, 336, 342, 675, 894
SstI GAGCTC 1 cut(s) 1719
StuI AGGCCT 3 cut(s) 250, 560, 673
StyD4I CCNGG 7 cut(s) 26, 245, 976, 1057, 1331, 1562, 1591
StyI CCWWGG 2 cut(s) 674, 775
TaaI ACNGT 4 cut(s) 1215, 1232, 1304, 1314
TaiI ACGT 1 cut(s) 1352
TaqI TCGA 3 cut(s) 171, 501, 1184
TasI AATT 8 cut(s) 223, 520, 681, 992, 1001, 1120, 1258, 1528
TatI WGTACW 3 cut(s) 539, 941, 1652
TauI GCSGC 1 cut(s) 1668
TfiI GAWTC 6 cut(s) 704, 904, 1499, 1643, 1685, 1696
Tru1I TTAA 3 cut(s) 227, 426, 1729
Tru9I TTAA 3 cut(s) 227, 426, 1729
TscAI CASTG 2 cut(s) 658, 1269
TseFI GTSAC 3 cut(s) 846, 1209, 1362
TseI GCWGC 5 cut(s) 138, 626, 1437, 1476, 1479
Tsp45I GTSAC 3 cut(s) 846, 1209, 1362
TspDTI ATGAA 6 cut(s) 17, 144, 201, 1101, 1440, 1602
TspGWI ACGGA 1 cut(s) 1116
TspRI CASTG 2 cut(s) 658, 1269
Van91I CCANNNNNTGG 1 cut(s) 781
XapI RAATTY 4 cut(s) 520, 681, 992, 1120
XceI RCATGY 3 cut(s) 334, 1028, 1202
XcmI CCANNNNNNNNNTGG 2 cut(s) 782, 928
XhoI CTCGAG 1 cut(s) 1183
XmaJI CCTAGG 1 cut(s) 674
XmiI GTMKAC 2 cut(s) 501, 1054
XmnI GAANNNNTTC 2 cut(s) 1275, 1620
XspI CTAG 7 cut(s) 126, 252, 309, 336, 342, 675, 894
ZrmI AGTACT 2 cut(s) 943, 1654
Zsp2I ATGCAT 1 cut(s) 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.