Prupe.7G270200_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0012415
Physical Location & Seq
Forward (+)
22172956 .. 22176260
3305 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G270200.2

Sequence Viewer

Length: 1971 bp
ATGGATGTCCTAGGCCTATTCAATACCCGGATTGATATTAGTTCAATCGCAAGCAAATTTGAAAGAGTGAGGCCTTCATACGATATGAACCGATCGTTGTCGTCAAAACTCGCCAAGAAACCCTCTCCAGCTCCGTCTCCGTCTCCGGCACCGGTGACGGCGAACAAGAAGAAGAAGAAAAAGGAACAGAGCCCTCGAAATCCATTACAGGATCTCAATACCAGCAGCACCATTAGCAATAGCAACAATGGCAGCGAGGCTTCTTCTTCTATCTCTGTCGAAGCCCCTCGAGGTTGCCTCAGGTTCTTTCTCTCTCATTCTTCTTCTTCTTCTAATTCAAAAACTCCTATTTGTAGGCCTAAAACACTGTCCAAAACCCCTAAATCGGCTCCTGTTGTAAAGCCTTCAAGGCCATCAAAATCTAAGGACAACCGGTCCAAATGCAATGCTTTGGAAAACCCAGAGAAACTCACATCCCGGAATGCGCACAAATTTAAGAATAATTCTTCTTGTTTGTACCAGTGGCAGTCCGGGAACAAACCCAGTTCTAGAAATGGACAAAAGATGAAAACTTGCTCAGTTTTAAACTCAAATGGAAATTCCCGGAGTAAGTTCGAATCTGGGTCTGTGGTGGAAGATATTGTCAGGGTGGTTGGTAATGCCGGTCAGCCCACTGAGCTCAAATCATGTGGTATTGATGAAAATTTTACACCTTTGAGTAAGATAGTGACAGGGTTGGGTTTGGACTCCAAAACTGTTAAGGATGAGGATTTGCAACAGAATTCTAGCAAAAGTGATAGTAGAACTCCACCAGTTCAGGCCTCTGTATCTCCAGAGATACAATGTGGATCGTCTGCGGTGTCAACAGCCACACGTGCTTGTTATGCTACTGGCCATGTTCTTTCTGGGATCACTGACAAGAGGAAGTGCAGACCTAGAGGAATTCTCAATGTAGAAGAGAATGATTCAGGTTTCAGTAGAGGGAAGGCTTTAGGCAGTTTTGAGGATGATGATGACGATGGTGATGATGATGAAACTGGAAAAGGAGTTTTTGGCAATTTTGATGCTCCCATGGTTCCTTTACCTACTGAAGCTTCAATGCATTGGCTGTTATCACCGTGTAATGAGGAGGATGAGGATCACAAGGAGCATTCTGAAAAAAGTTTTCAGAATTCTGTAGAGTCTGTCAATCTTTATTCTCCATTTTCAATCTCAGGTCATCATGGATTTTCTTTAGATATATGCAACAATACTAATGTGGGAAGTACTACTAACTGTAGCCGGAGGAGTACTTCAATTTTTCCAAGCTATCAAGAAGTGTTAGAGCCCTTAAATACCGATCCTGTTTTGTCGTCTCCCCCTTGCACACCTGGTTGCGAGGCTCTAACCTTGAAAGACGAAAGAAAATACCGTTATGATTATGGCGGCGATAATTCTCCATTCTCTATGGTTTCACTGGGTAGTGAAAATGTTATTCGTACTCCTCTATCACACTCAAGTATGGACAATCAAAGGAAACACTACTTTGATTCTGAACTCAGTTCAGTGGCTGAAGCTATTCGGATGGCAAGCTTGTCCCCAAACAGTAATGTATTAGTTGAGGATCAGATTGATTCAAGTTTCCAGTTTGACTGTCTAACTACAACTTGCAATTCAATCAGTCGATTTCAGAAAGTTTTGGATGATCGGGCTTCCTGGCTTTCCAACTCTACACCAGAAAATGTGTCACAATCCGAGATGAGGATATCATGGAGAGAAGGGTTAGTGAGTCGTATCTACGATATGGATGAATATGATTGCTGCAGGTGTTTGTCAGACGAAGAGGAAGATATCAATGGCTGCAGCAGCGGCTTTCCTTCTCAGTCATCATGCGTGTGTGCTGAGTCCATAAGCACTGATGGAGGTGGCCTGCTTGCTTCAGGGGATTCAGATTGGACTCTCTGCTACAAGAACGAGTTGTTCCATGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

657

Amino Acids

71.6

Weight (kDa)

5.72

Isoelectric Point (pI)

56.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012415)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43990
fragaria_vesca FvH4_1g00220
malus_domestica MD00G1086900.v1.1 MD15G1142800.v1.1
prunus_persica Prupe.7G270200_v2.0.a1 Prupe.7G270200_v2.0.a1
pyrus_communis pycom15g12890 pycom290g00170
rosa_chinensis RchiOBHm_Chr2g0084511
rosa_laevigata RLG00000015623
rosa_multiflora Rmu_co8107158.1_g000001 Rmu_sc0009647.1_g000004
rosa_roxburghii Rroxscaffold_2G00156000
rosa_rugosa Rorug01G0454600
rosa_samantha Rh2AG002700 Rh2BG002300 Rh2CG002800 Rh2DG002800
rosa_wichuraiana Rw2G000240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1794
AbsI CCTCGAGG 1 cut(s) 288
Acc16I TGCGCA 1 cut(s) 486
Acc36I ACCTGC 1 cut(s) 1794
AccB1I GGYRCC 1 cut(s) 148
AciI CCGC 3 cut(s) 857, 1425, 1848
AclWI GGATC 6 cut(s) 219, 856, 917, 1146, 1334, 1611
AcoI YGGCCR 1 cut(s) 892
AcsI RAATTY 7 cut(s) 56, 491, 598, 703, 781, 942, 1171
AcuI CTGAAG 3 cut(s) 1110, 1572, 1902
AcvI CACGTG 1 cut(s) 875
AfaI GTAC 4 cut(s) 518, 1267, 1291, 1480
AfiI CCNNNNNNNGG 2 cut(s) 385, 1740
AflIII ACRYGT 1 cut(s) 872
AgeI ACCGGT 2 cut(s) 151, 432
AhdI GACNNNNNGTC 1 cut(s) 433
AjnI CCWGG 2 cut(s) 1369, 1694
AluBI AGCT 6 cut(s) 131, 679, 1094, 1308, 1556, 1572
AluI AGCT 6 cut(s) 131, 679, 1094, 1308, 1556, 1572
Alw21I GWGCWC 1 cut(s) 681
Alw26I GTCTC 3 cut(s) 141, 147, 1359
AlwI GGATC 6 cut(s) 219, 856, 917, 1146, 1334, 1611
AlwNI CAGNNNCTG 1 cut(s) 1550
Ama87I CYCGRG 1 cut(s) 288
AoxI GGCC 7 cut(s) 13, 71, 356, 410, 819, 892, 1906
ApeKI GCWGC 6 cut(s) 225, 252, 1800, 1839, 1842, 1845
ApoI RAATTY 7 cut(s) 56, 491, 598, 703, 781, 942, 1171
AsiGI ACCGGT 2 cut(s) 151, 432
Asp700I GAANNNNTTC 1 cut(s) 1557
AspA2I CCTAGG 1 cut(s) 10
AspLEI GCGC 1 cut(s) 487
AspS9I GGNCC 1 cut(s) 435
AsuC2I CCSGG 4 cut(s) 28, 478, 532, 604
AsuHPI GGTGA 3 cut(s) 166, 1034, 1107
AsuII TTCGAA 1 cut(s) 615
AvaI CYCGRG 1 cut(s) 288
AvaII GGWCC 1 cut(s) 435
AvrII CCTAGG 1 cut(s) 10
AxyI CCTNAGG 1 cut(s) 299
BalI TGGCCA 1 cut(s) 894
BanI GGYRCC 1 cut(s) 148
BanII GRGCYC 3 cut(s) 194, 681, 1329
BbrPI CACGTG 1 cut(s) 875
Bbv12I GWGCWC 1 cut(s) 681
BbvI GCAGC 6 cut(s) 237, 264, 1787, 1826, 1854, 1857
BccI CCATC 4 cut(s) 421, 1013, 1558, 1892
BceAI ACGGC 1 cut(s) 174
BciT130I CCWGG 2 cut(s) 1371, 1696
BcnI CCSGG 4 cut(s) 28, 478, 532, 604
BcoDI GTCTC 3 cut(s) 141, 147, 1359
BfaI CTAG 4 cut(s) 11, 549, 786, 936
BfmI CTRYAG 4 cut(s) 1176, 1276, 1801, 1840
BfuAI ACCTGC 1 cut(s) 1794
BglI GCCNNNNNGGC 1 cut(s) 409
BisI GCNGC 8 cut(s) 226, 253, 1426, 1801, 1840, 1843, 1846, 1849
BlnI CCTAGG 1 cut(s) 10
BlsI GCNGC 8 cut(s) 227, 254, 1427, 1802, 1841, 1844, 1847, 1850
BmcAI AGTACT 2 cut(s) 1267, 1291
Bme1390I CCNGG 6 cut(s) 28, 478, 532, 604, 1371, 1696
Bme18I GGWCC 1 cut(s) 435
BmeRI GACNNNNNGTC 1 cut(s) 433
BmeT110I CYCGRG 1 cut(s) 288
BmgT120I GGNCC 1 cut(s) 435
BmiI GGNNCC 3 cut(s) 150, 390, 1077
BmrFI CCNGG 6 cut(s) 28, 478, 532, 604, 1371, 1696
BmrI ACTGGG 2 cut(s) 537, 1466
BmsI GCATC 1 cut(s) 1054
BmuI ACTGGG 2 cut(s) 537, 1466
BplI GAGNNNNNCTC 4 cut(s) 282, 314, 930, 962
BpmI CTGGAG 2 cut(s) 111, 816
Bpu14I TTCGAA 1 cut(s) 615
BpuEI CTTGAG 1 cut(s) 1480
BpuMI CCSGG 4 cut(s) 28, 478, 532, 604
BsaAI YACGTR 1 cut(s) 875
BsaBI GATNNNNATC 1 cut(s) 1137
BsaJI CCNNGG 2 cut(s) 10, 1071
BsaWI WCCGGW 2 cut(s) 151, 432
Bsc4I CCNNNNNNNGG 2 cut(s) 385, 1740
Bse118I RCCGGY 3 cut(s) 151, 432, 662
Bse1I ACTGG 7 cut(s) 520, 543, 812, 895, 1042, 1461, 1624
Bse21I CCTNAGG 1 cut(s) 299
Bse3DI GCAATG 1 cut(s) 451
Bse8I GATNNNNATC 1 cut(s) 1137
BseBI CCWGG 2 cut(s) 1371, 1696
BseDI CCNNGG 2 cut(s) 10, 1071
BseGI GGATG 8 cut(s) 10, 473, 769, 1012, 1138, 1569, 1687, 1792
BseJI GATNNNNATC 1 cut(s) 1137
BseLI CCNNNNNNNGG 2 cut(s) 385, 1740
BseMI GCAATG 1 cut(s) 451
BseMII CTCAG 7 cut(s) 313, 591, 666, 1227, 1552, 1872, 1874
BseNI ACTGG 7 cut(s) 520, 543, 812, 895, 1042, 1461, 1624
BseRI GAGGAG 3 cut(s) 1142, 1300, 1473
BseXI GCAGC 6 cut(s) 237, 264, 1787, 1826, 1854, 1857
BsgI GTGCAG 1 cut(s) 949
Bsh1285I CGRYCG 1 cut(s) 95
BshFI GGCC 7 cut(s) 15, 73, 358, 412, 821, 894, 1908
BshNI GGYRCC 1 cut(s) 148
BshTI ACCGGT 2 cut(s) 151, 432
BsiEI CGRYCG 1 cut(s) 95
BsiHKAI GWGCWC 1 cut(s) 681
BsiHKCI CYCGRG 1 cut(s) 288
BsiSI CCGG 9 cut(s) 28, 146, 152, 433, 478, 531, 604, 663, 1282
BslFI GGGAC 1 cut(s) 1561
BslI CCNNNNNNNGG 2 cut(s) 385, 1740
BsmAI GTCTC 3 cut(s) 141, 147, 1359
BsmBI CGTCTC 3 cut(s) 141, 147, 1359
BsmFI GGGAC 1 cut(s) 1561
BsmI GAATGC 2 cut(s) 487, 1150
BsnI GGCC 7 cut(s) 15, 73, 358, 412, 821, 894, 1908
BsoBI CYCGRG 1 cut(s) 288
Bsp119I TTCGAA 1 cut(s) 615
Bsp1286I GDGCHC 3 cut(s) 194, 681, 1329
Bsp143I GATC 8 cut(s) 92, 211, 848, 909, 1138, 1339, 1603, 1684
Bsp19I CCATGG 1 cut(s) 1071
BspACI CCGC 3 cut(s) 857, 1425, 1848
BspANI GGCC 7 cut(s) 15, 73, 358, 412, 821, 894, 1908
BspCNI CTCAG 7 cut(s) 312, 590, 667, 1226, 1551, 1873, 1873
BspLI GGNNCC 3 cut(s) 150, 390, 1077
BspMAI CTGCAG 2 cut(s) 1805, 1844
BspMI ACCTGC 1 cut(s) 1794
BspPI GGATC 6 cut(s) 219, 856, 917, 1146, 1334, 1611
BspT104I TTCGAA 1 cut(s) 615
BspT107I GGYRCC 1 cut(s) 148
BsrDI GCAATG 1 cut(s) 451
BsrFI RCCGGY 3 cut(s) 151, 432, 662
BsrI ACTGG 7 cut(s) 520, 543, 812, 895, 1042, 1461, 1624
BssAI RCCGGY 3 cut(s) 151, 432, 662
BssECI CCNNGG 2 cut(s) 10, 1071
BssMI GATC 8 cut(s) 92, 211, 848, 909, 1138, 1339, 1603, 1684
BssT1I CCWWGG 2 cut(s) 10, 1071
Bst2UI CCWGG 2 cut(s) 1371, 1696
Bst4CI ACNGT 7 cut(s) 369, 757, 1119, 1277, 1412, 1586, 1634
Bst6I CTCTTC 2 cut(s) 951, 1815
BstBAI YACGTR 1 cut(s) 875
BstBI TTCGAA 1 cut(s) 615
BstC8I GCNNGC 4 cut(s) 52, 1570, 1910, 1914
BstDEI CTNAG 8 cut(s) 299, 423, 577, 675, 1213, 1538, 1860, 1881
BstDSI CCRYGG 1 cut(s) 1071
BstF5I GGATG 8 cut(s) 10, 473, 769, 1012, 1138, 1569, 1687, 1792
BstHHI GCGC 1 cut(s) 487
BstKTI GATC 8 cut(s) 95, 214, 851, 912, 1141, 1342, 1606, 1687
BstMAI GTCTC 3 cut(s) 141, 147, 1359
BstMBI GATC 8 cut(s) 92, 211, 848, 909, 1138, 1339, 1603, 1684
BstMCI CGRYCG 1 cut(s) 95
BstMWI GCNNNNNNNGC 8 cut(s) 234, 249, 409, 676, 875, 884, 1845, 1848
BstNI CCWGG 2 cut(s) 1371, 1696
BstSCI CCNGG 6 cut(s) 26, 476, 530, 602, 1369, 1694
BstSFI CTRYAG 4 cut(s) 1176, 1276, 1801, 1840
BstV1I GCAGC 6 cut(s) 237, 264, 1787, 1826, 1854, 1857
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
Bsu36I CCTNAGG 1 cut(s) 299
BsuRI GGCC 7 cut(s) 15, 73, 358, 412, 821, 894, 1908
BtgI CCRYGG 1 cut(s) 1071
BtsCI GGATG 8 cut(s) 10, 473, 769, 1012, 1138, 1569, 1687, 1792
BtsIMutI CAGTG 7 cut(s) 365, 527, 672, 912, 1454, 1551, 1893
BveI ACCTGC 1 cut(s) 1794
Cac8I GCNNGC 4 cut(s) 52, 1570, 1910, 1914
CaiI CAGNNNCTG 1 cut(s) 1550
CfoI GCGC 1 cut(s) 487
Cfr10I RCCGGY 3 cut(s) 151, 432, 662
Cfr13I GGNCC 1 cut(s) 435
CsiI ACCWGGT 1 cut(s) 1369
Csp6I GTAC 4 cut(s) 517, 1266, 1290, 1479
CspAI ACCGGT 2 cut(s) 151, 432
CspCI CAANNNNNGTGG 2 cut(s) 670, 705
CviAII CATG 7 cut(s) 687, 896, 1072, 1223, 1749, 1869, 1964
CviQI GTAC 4 cut(s) 517, 1266, 1290, 1479
DdeI CTNAG 8 cut(s) 299, 423, 577, 675, 1213, 1538, 1860, 1881
DpnI GATC 8 cut(s) 94, 213, 850, 911, 1140, 1341, 1605, 1686
DpnII GATC 8 cut(s) 92, 211, 848, 909, 1138, 1339, 1603, 1684
DraI TTTAAA 1 cut(s) 585
DriI GACNNNNNGTC 1 cut(s) 433
EaeI YGGCCR 1 cut(s) 892
Eam1104I CTCTTC 2 cut(s) 951, 1815
Eam1105I GACNNNNNGTC 1 cut(s) 433
EarI CTCTTC 2 cut(s) 951, 1815
Ecl136II GAGCTC 1 cut(s) 679
Eco130I CCWWGG 2 cut(s) 10, 1071
Eco147I AGGCCT 4 cut(s) 15, 73, 358, 821
Eco24I GRGCYC 3 cut(s) 194, 681, 1329
Eco32I GATATC 2 cut(s) 1746, 1831
Eco47I GGWCC 1 cut(s) 435
Eco53kI GAGCTC 1 cut(s) 679
Eco57I CTGAAG 3 cut(s) 1110, 1572, 1902
Eco72I CACGTG 1 cut(s) 875
Eco81I CCTNAGG 1 cut(s) 299
Eco88I CYCGRG 1 cut(s) 288
EcoICRI GAGCTC 1 cut(s) 679
EcoRI GAATTC 3 cut(s) 781, 942, 1171
EcoRII CCWGG 2 cut(s) 1369, 1694
EcoRV GATATC 2 cut(s) 1746, 1831
EcoT14I CCWWGG 2 cut(s) 10, 1071
EcoT22I ATGCAT 1 cut(s) 1104
EcoT38I GRGCYC 3 cut(s) 194, 681, 1329
ErhI CCWWGG 2 cut(s) 10, 1071
Esp3I CGTCTC 3 cut(s) 141, 147, 1359
FaeI CATG 7 cut(s) 690, 899, 1075, 1226, 1752, 1872, 1967
FaqI GGGAC 1 cut(s) 1561
FatI CATG 7 cut(s) 686, 895, 1071, 1222, 1748, 1868, 1963
Fnu4HI GCNGC 8 cut(s) 226, 253, 1426, 1801, 1840, 1843, 1846, 1849
FokI GGATG 8 cut(s) 17, 460, 776, 1019, 1145, 1576, 1694, 1799
FriOI GRGCYC 3 cut(s) 194, 681, 1329
Fsp4HI GCNGC 8 cut(s) 226, 253, 1426, 1801, 1840, 1843, 1846, 1849
FspAI RTGCGCAY 1 cut(s) 486
FspBI CTAG 4 cut(s) 11, 549, 786, 936
FspI TGCGCA 1 cut(s) 486
GlaI GCGC 1 cut(s) 486
GluI GCNGC 8 cut(s) 226, 253, 1426, 1801, 1840, 1843, 1846, 1849
GsuI CTGGAG 2 cut(s) 111, 816
HaeIII GGCC 7 cut(s) 15, 73, 358, 412, 821, 894, 1908
HapII CCGG 9 cut(s) 28, 146, 152, 433, 478, 531, 604, 663, 1282
HhaI GCGC 1 cut(s) 487
Hin1II CATG 7 cut(s) 690, 899, 1075, 1226, 1752, 1872, 1967
Hin6I GCGC 1 cut(s) 485
HinP1I GCGC 1 cut(s) 485
HincII GTYRAC 1 cut(s) 864
HindII GTYRAC 1 cut(s) 864
HindIII AAGCTT 2 cut(s) 1092, 1570
HpaII CCGG 9 cut(s) 28, 146, 152, 433, 478, 531, 604, 663, 1282
HphI GGTGA 3 cut(s) 166, 1034, 1107
Hpy166II GTNNAC 1 cut(s) 864
Hpy188I TCNGA 9 cut(s) 1156, 1170, 1534, 1563, 1608, 1671, 1735, 1816, 1930
Hpy188III TCNNGA 3 cut(s) 549, 833, 1313
Hpy8I GTNNAC 1 cut(s) 864
HpyAV CCTTC 5 cut(s) 84, 414, 979, 1751, 1866
HpyCH4III ACNGT 7 cut(s) 369, 757, 1119, 1277, 1412, 1586, 1634
HpyCH4IV ACGT 1 cut(s) 874
HpyCH4V TGCA 9 cut(s) 444, 775, 930, 1102, 1245, 1365, 1650, 1803, 1842
HpyF10VI GCNNNNNNNGC 8 cut(s) 234, 249, 409, 676, 875, 884, 1845, 1848
HpyF3I CTNAG 8 cut(s) 299, 423, 577, 675, 1213, 1538, 1860, 1881
HpySE526I ACGT 1 cut(s) 874
Hsp92II CATG 7 cut(s) 690, 899, 1075, 1226, 1752, 1872, 1967
HspAI GCGC 1 cut(s) 485
Kzo9I GATC 8 cut(s) 92, 211, 848, 909, 1138, 1339, 1603, 1684
LmnI GCTCC 4 cut(s) 136, 394, 1072, 1147
Lsp1109I GCAGC 6 cut(s) 237, 264, 1787, 1826, 1854, 1857
LweI GCATC 1 cut(s) 1054
MabI ACCWGGT 1 cut(s) 1369
MaeI CTAG 4 cut(s) 11, 549, 786, 936
MaeII ACGT 1 cut(s) 874
MaeIII GTNAC 3 cut(s) 154, 727, 1725
MalI GATC 8 cut(s) 94, 213, 850, 911, 1140, 1341, 1605, 1686
MboI GATC 8 cut(s) 92, 211, 848, 909, 1138, 1339, 1603, 1684
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 3 cut(s) 194, 681, 1329
MlsI TGGCCA 1 cut(s) 894
MluNI TGGCCA 1 cut(s) 894
MlyI GAGTC 5 cut(s) 740, 1190, 1777, 1892, 1930
MmeI TCCRAC 1 cut(s) 1728
Mox20I TGGCCA 1 cut(s) 894
Mph1103I ATGCAT 1 cut(s) 1104
MroXI GAANNNNTTC 1 cut(s) 1557
MscI TGGCCA 1 cut(s) 894
MseI TTAA 4 cut(s) 495, 584, 759, 1331
MslI CAYNNNNRTG 1 cut(s) 1873
Msp20I TGGCCA 1 cut(s) 894
MspA1I CMGCKG 1 cut(s) 1848
MspI CCGG 9 cut(s) 28, 146, 152, 433, 478, 531, 604, 663, 1282
MspR9I CCNGG 6 cut(s) 28, 478, 532, 604, 1371, 1696
Mva1269I GAATGC 2 cut(s) 487, 1150
MvaI CCWGG 2 cut(s) 1371, 1696
MwoI GCNNNNNNNGC 8 cut(s) 234, 249, 409, 676, 875, 884, 1845, 1848
NciI CCSGG 4 cut(s) 28, 478, 532, 604
NcoI CCATGG 1 cut(s) 1071
NdeII GATC 8 cut(s) 92, 211, 848, 909, 1138, 1339, 1603, 1684
NlaIII CATG 7 cut(s) 690, 899, 1075, 1226, 1752, 1872, 1967
NlaIV GGNNCC 3 cut(s) 150, 390, 1077
NmuCI GTSAC 3 cut(s) 154, 727, 1725
NsbI TGCGCA 1 cut(s) 486
NsiI ATGCAT 1 cut(s) 1104
NspV TTCGAA 1 cut(s) 615
PaeR7I CTCGAG 1 cut(s) 288
PaqCI CACCTGC 1 cut(s) 1794
PceI AGGCCT 4 cut(s) 15, 73, 358, 821
PctI GAATGC 2 cut(s) 487, 1150
PdmI GAANNNNTTC 1 cut(s) 1557
PfeI GAWTC 5 cut(s) 617, 965, 1529, 1613, 1925
PfoI TCCNGGA 3 cut(s) 476, 530, 602
PinAI ACCGGT 2 cut(s) 151, 432
PkrI GCNGC 8 cut(s) 227, 254, 1427, 1802, 1841, 1844, 1847, 1850
Ple19I CGATCG 1 cut(s) 95
PleI GAGTC 5 cut(s) 740, 1189, 1776, 1891, 1930
PmaCI CACGTG 1 cut(s) 875
PmlI CACGTG 1 cut(s) 875
PpsI GAGTC 5 cut(s) 740, 1189, 1776, 1891, 1930
Ppu21I YACGTR 1 cut(s) 875
Psp124BI GAGCTC 1 cut(s) 681
Psp6I CCWGG 2 cut(s) 1369, 1694
PspCI CACGTG 1 cut(s) 875
PspGI CCWGG 2 cut(s) 1369, 1694
PspN4I GGNNCC 3 cut(s) 150, 390, 1077
PspPI GGNCC 1 cut(s) 435
PspXI VCTCGAGB 1 cut(s) 288
PstI CTGCAG 2 cut(s) 1805, 1844
PstNI CAGNNNCTG 1 cut(s) 1550
PsuI RGATCY 1 cut(s) 211
PvuI CGATCG 1 cut(s) 95
RsaI GTAC 4 cut(s) 518, 1267, 1291, 1480
RsaNI GTAC 4 cut(s) 517, 1266, 1290, 1479
RseI CAYNNNNRTG 1 cut(s) 1873
SacI GAGCTC 1 cut(s) 681
SaqAI TTAA 4 cut(s) 495, 584, 759, 1331
SatI GCNGC 8 cut(s) 226, 253, 1426, 1801, 1840, 1843, 1846, 1849
Sau3AI GATC 8 cut(s) 92, 211, 848, 909, 1138, 1339, 1603, 1684
Sau96I GGNCC 1 cut(s) 435
ScaI AGTACT 2 cut(s) 1267, 1291
SchI GAGTC 5 cut(s) 740, 1190, 1777, 1892, 1930
ScrFI CCNGG 6 cut(s) 28, 478, 532, 604, 1371, 1696
SduI GDGCHC 3 cut(s) 194, 681, 1329
SexAI ACCWGGT 1 cut(s) 1369
SfaNI GCATC 1 cut(s) 1054
SfcI CTRYAG 4 cut(s) 1176, 1276, 1801, 1840
Sfr274I CTCGAG 1 cut(s) 288
SfuI TTCGAA 1 cut(s) 615
SgrAI CRCCGGYG 1 cut(s) 151
SinI GGWCC 1 cut(s) 435
SlaI CTCGAG 1 cut(s) 288
SmiMI CAYNNNNRTG 1 cut(s) 1873
SmlI CTYRAG 2 cut(s) 288, 1495
SmoI CTYRAG 2 cut(s) 288, 1495
SseBI AGGCCT 4 cut(s) 15, 73, 358, 821
SsiI CCGC 3 cut(s) 857, 1425, 1848
SspMI CTAG 4 cut(s) 11, 549, 786, 936
SstI GAGCTC 1 cut(s) 681
StuI AGGCCT 4 cut(s) 15, 73, 358, 821
StyD4I CCNGG 6 cut(s) 26, 476, 530, 602, 1369, 1694
StyI CCWWGG 2 cut(s) 10, 1071
TaaI ACNGT 7 cut(s) 369, 757, 1119, 1277, 1412, 1586, 1634
TaiI ACGT 1 cut(s) 877
TaqI TCGA 5 cut(s) 196, 279, 289, 615, 1663
TatI WGTACW 2 cut(s) 1265, 1289
TauI GCSGC 2 cut(s) 1428, 1851
TfiI GAWTC 5 cut(s) 617, 965, 1529, 1613, 1925
Tru1I TTAA 4 cut(s) 495, 584, 759, 1331
Tru9I TTAA 4 cut(s) 495, 584, 759, 1331
TscAI CASTG 7 cut(s) 372, 527, 679, 919, 1461, 1551, 1900
TseFI GTSAC 3 cut(s) 154, 727, 1725
TseI GCWGC 6 cut(s) 225, 252, 1800, 1839, 1842, 1845
Tsp45I GTSAC 3 cut(s) 154, 727, 1725
TspDTI ATGAA 6 cut(s) 66, 101, 581, 714, 1047, 1803
TspGWI ACGGA 2 cut(s) 123, 129
TspRI CASTG 7 cut(s) 372, 527, 679, 919, 1461, 1551, 1900
VpaK11BI GGWCC 1 cut(s) 435
XapI RAATTY 7 cut(s) 56, 491, 598, 703, 781, 942, 1171
XbaI TCTAGA 1 cut(s) 548
XcmI CCANNNNNNNNNTGG 1 cut(s) 902
XhoI CTCGAG 1 cut(s) 288
XmaJI CCTAGG 1 cut(s) 10
XmnI GAANNNNTTC 1 cut(s) 1557
XspI CTAG 4 cut(s) 11, 549, 786, 936
ZrmI AGTACT 2 cut(s) 1267, 1291
Zsp2I ATGCAT 1 cut(s) 1104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.