Rmu_sc0009647.1_g000004

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009647.1
OG0012415
Physical Location & Seq
Reverse (-)
16345 .. 17325
981 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009647.1_g000004.1.cds

Sequence Viewer

Length: 981 bp
atgaaccgatcgttgtcgtcaaaggcggcgaagaagccctctccggcgccggcgaagaagaacaaaaaagagcagagccttcgaaacccgttgcaggatctcaatgtcattagcaccactactagtactagcaaccatgggagcgaggcttcttcttctgcttcaatgtccatcgaagccccacgaggttgcctcaggttctttctctctcactcctcttcctctaatttaaaaacccctatacgcacccgcaccaggcctaaaactctctccaaaacccctaaatcagctcctcttgtgaggcctaaggagaacccctccaattccaaatgcaatgtttccaaacacaacccacagaaatcaaaattcaagactagttcttggtctaatacagacaaccagaggaaacactatgattctgaactcagttcagtggctgaagctattcggatggcaagcttgtctccaaaccgtcatgaactgattgaggatcagattgattcaagtttccaatttgattgtctaactacaacttgtaattcgatcagtcgaatccagaaaatgttggatgatcaggctccctggcattccaactccactttagagaatgtgtcacaatcccagctgaggatatcatggagagaagggttgatgagccgtatgcatgacatggatgaatatgattgctgcagatgtttatcagacgaagaggaagatatcaatggctgcagcaatgaccttcccaaaaagtcctgtctaagtcctgaaatcaccaacaatgttgaggcagatcagattttaactgatacttcctggtctgctgatatactggatgatgaaccaggagttgcgggaaaagttaaagaagactttcctcctcaatcatgtgccgagtccataagcactgatggaggcggcctgcttgcttcaggggattcagactggaatctctgctacaagaatgagctgttccatgtttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

326

Amino Acids

36.14

Weight (kDa)

6.26

Isoelectric Point (pI)

64.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012415)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43990
fragaria_vesca FvH4_1g00220
malus_domestica MD00G1086900.v1.1 MD15G1142800.v1.1
prunus_persica Prupe.7G270200_v2.0.a1 Prupe.7G270200_v2.0.a1
pyrus_communis pycom15g12890 pycom290g00170
rosa_chinensis RchiOBHm_Chr2g0084511
rosa_laevigata RLG00000015623
rosa_multiflora Rmu_co8107158.1_g000001 Rmu_sc0009647.1_g000004
rosa_roxburghii Rroxscaffold_2G00156000
rosa_rugosa Rorug01G0454600
rosa_samantha Rh2AG002700 Rh2BG002300 Rh2CG002800 Rh2DG002800
rosa_wichuraiana Rw2G000240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 46
AciI CCGC 4 cut(s) 26, 250, 851, 915
AclWI GGATC 2 cut(s) 105, 498
AcsI RAATTY 1 cut(s) 365
AcuI CTGAAG 2 cut(s) 459, 912
AcyI GRCGYC 1 cut(s) 47
AfaI GTAC 1 cut(s) 127
AfiI CCNNNNNNNGG 3 cut(s) 94, 255, 627
AgsI TTSAA 3 cut(s) 165, 370, 504
AhlI ACTAGT 2 cut(s) 122, 374
AjnI CCWGG 4 cut(s) 254, 581, 812, 841
AluBI AGCT 5 cut(s) 290, 443, 459, 625, 967
AluI AGCT 5 cut(s) 290, 443, 459, 625, 967
Alw26I GTCTC 1 cut(s) 468
AlwI GGATC 2 cut(s) 105, 498
AlwNI CAGNNNCTG 1 cut(s) 437
AoxI GGCC 3 cut(s) 257, 302, 916
ApeKI GCWGC 3 cut(s) 687, 726, 729
ApoI RAATTY 1 cut(s) 365
Asp700I GAANNNNTTC 2 cut(s) 444, 870
AspLEI GCGC 1 cut(s) 49
AsuHPI GGTGA 1 cut(s) 763
AsuII TTCGAA 1 cut(s) 82
AxyI CCTNAGG 2 cut(s) 194, 306
BanI GGYRCC 1 cut(s) 46
BauI CACGAG 1 cut(s) 183
BbsI GAAGAC 1 cut(s) 873
BbvCI CCTCAGC 1 cut(s) 626
BbvI GCAGC 3 cut(s) 674, 713, 741
BccI CCATC 3 cut(s) 179, 445, 902
BceAI ACGGC 1 cut(s) 642
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciT130I CCWGG 4 cut(s) 256, 583, 814, 843
BclI TGATCA 1 cut(s) 571
BcoDI GTCTC 1 cut(s) 468
BcuI ACTAGT 2 cut(s) 122, 374
BfaI CTAG 3 cut(s) 123, 129, 375
BfmI CTRYAG 2 cut(s) 688, 727
BfoI RGCGCY 1 cut(s) 50
BisI GCNGC 5 cut(s) 27, 688, 727, 730, 916
BlsI GCNGC 5 cut(s) 28, 689, 728, 731, 917
BmcAI AGTACT 1 cut(s) 127
Bme1390I CCNGG 4 cut(s) 256, 583, 814, 843
BmiI GGNNCC 2 cut(s) 48, 579
BmrFI CCNGG 4 cut(s) 256, 583, 814, 843
BpiI GAAGAC 1 cut(s) 873
BplI GAGNNNNNCTC 4 cut(s) 177, 209, 302, 334
Bpu10I CCTNAGC 1 cut(s) 626
Bpu14I TTCGAA 1 cut(s) 82
BsaBI GATNNNNATC 1 cut(s) 697
BsaHI GRCGYC 1 cut(s) 47
BsaJI CCNNGG 2 cut(s) 136, 581
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 255, 627
Bse118I RCCGGY 1 cut(s) 49
Bse1I ACTGG 2 cut(s) 834, 947
Bse21I CCTNAGG 2 cut(s) 194, 306
Bse3DI GCAATG 2 cut(s) 340, 739
Bse8I GATNNNNATC 1 cut(s) 697
BseBI CCWGG 4 cut(s) 256, 583, 814, 843
BseDI CCNNGG 2 cut(s) 136, 581
BseGI GGATG 4 cut(s) 456, 574, 679, 838
BseJI GATNNNNATC 1 cut(s) 697
BseLI CCNNNNNNNGG 3 cut(s) 94, 255, 627
BseMI GCAATG 2 cut(s) 340, 739
BseMII CTCAG 3 cut(s) 208, 439, 617
BseNI ACTGG 2 cut(s) 834, 947
BseRI GAGGAG 3 cut(s) 205, 282, 867
BseXI GCAGC 3 cut(s) 674, 713, 741
BseYI CCCAGC 1 cut(s) 621
Bsh1285I CGRYCG 1 cut(s) 11
BshFI GGCC 3 cut(s) 259, 304, 918
BshNI GGYRCC 1 cut(s) 46
BsiEI CGRYCG 1 cut(s) 11
BsiSI CCGG 2 cut(s) 44, 50
BslI CCNNNNNNNGG 3 cut(s) 94, 255, 627
BsmAI GTCTC 1 cut(s) 468
BsmI GAATGC 1 cut(s) 586
BsnI GGCC 3 cut(s) 259, 304, 918
Bsp119I TTCGAA 1 cut(s) 82
Bsp143I GATC 6 cut(s) 8, 97, 490, 543, 571, 790
Bsp19I CCATGG 1 cut(s) 136
BspACI CCGC 4 cut(s) 26, 250, 851, 915
BspANI GGCC 3 cut(s) 259, 304, 918
BspCNI CTCAG 3 cut(s) 207, 438, 618
BspHI TCATGA 1 cut(s) 475
BspLI GGNNCC 2 cut(s) 48, 579
BspMAI CTGCAG 2 cut(s) 692, 731
BspPI GGATC 2 cut(s) 105, 498
BspT104I TTCGAA 1 cut(s) 82
BspT107I GGYRCC 1 cut(s) 46
BsrDI GCAATG 2 cut(s) 340, 739
BsrFI RCCGGY 1 cut(s) 49
BsrI ACTGG 2 cut(s) 834, 947
BssAI RCCGGY 1 cut(s) 49
BssECI CCNNGG 2 cut(s) 136, 581
BssMI GATC 6 cut(s) 8, 97, 490, 543, 571, 790
BssNI GRCGYC 1 cut(s) 47
BssSI CACGAG 1 cut(s) 183
BssT1I CCWWGG 1 cut(s) 136
Bst2BI CACGAG 1 cut(s) 183
Bst2UI CCWGG 4 cut(s) 256, 583, 814, 843
Bst4CI ACNGT 1 cut(s) 473
Bst6I CTCTTC 2 cut(s) 223, 702
BstACI GRCGYC 1 cut(s) 47
BstBI TTCGAA 1 cut(s) 82
BstC8I GCNNGC 4 cut(s) 51, 457, 920, 924
BstDEI CTNAG 5 cut(s) 194, 306, 425, 626, 758
BstDSI CCRYGG 1 cut(s) 136
BstF5I GGATG 4 cut(s) 456, 574, 679, 838
BstH2I RGCGCY 1 cut(s) 50
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 6 cut(s) 11, 100, 493, 546, 574, 793
BstMAI GTCTC 1 cut(s) 468
BstMBI GATC 6 cut(s) 8, 97, 490, 543, 571, 790
BstMCI CGRYCG 1 cut(s) 11
BstNI CCWGG 4 cut(s) 256, 583, 814, 843
BstSCI CCNGG 4 cut(s) 254, 581, 812, 841
BstSFI CTRYAG 2 cut(s) 688, 727
BstV1I GCAGC 3 cut(s) 674, 713, 741
BstV2I GAAGAC 1 cut(s) 873
BstX2I RGATCY 1 cut(s) 97
BstYI RGATCY 1 cut(s) 97
Bsu36I CCTNAGG 2 cut(s) 194, 306
BsuRI GGCC 3 cut(s) 259, 304, 918
BtgI CCRYGG 1 cut(s) 136
BtsCI GGATG 4 cut(s) 456, 574, 679, 838
BtsIMutI CAGTG 2 cut(s) 438, 903
Cac8I GCNNGC 4 cut(s) 51, 457, 920, 924
CaiI CAGNNNCTG 1 cut(s) 437
CciI TCATGA 1 cut(s) 475
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 1 cut(s) 49
Csp6I GTAC 1 cut(s) 126
CviAII CATG 7 cut(s) 137, 476, 636, 665, 670, 885, 974
CviQI GTAC 1 cut(s) 126
DdeI CTNAG 5 cut(s) 194, 306, 425, 626, 758
DinI GGCGCC 1 cut(s) 48
DpnI GATC 6 cut(s) 10, 99, 492, 545, 573, 792
DpnII GATC 6 cut(s) 8, 97, 490, 543, 571, 790
DraI TTTAAA 1 cut(s) 231
Eam1104I CTCTTC 2 cut(s) 223, 702
EarI CTCTTC 2 cut(s) 223, 702
Eco130I CCWWGG 1 cut(s) 136
Eco147I AGGCCT 2 cut(s) 259, 304
Eco32I GATATC 2 cut(s) 633, 718
Eco57I CTGAAG 2 cut(s) 459, 912
Eco81I CCTNAGG 2 cut(s) 194, 306
EcoRII CCWGG 4 cut(s) 254, 581, 812, 841
EcoRV GATATC 2 cut(s) 633, 718
EcoT14I CCWWGG 1 cut(s) 136
EcoT22I ATGCAT 1 cut(s) 666
EgeI GGCGCC 1 cut(s) 48
EheI GGCGCC 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 7 cut(s) 140, 479, 639, 668, 673, 888, 977
FatI CATG 7 cut(s) 136, 475, 635, 664, 669, 884, 973
FauI CCCGC 2 cut(s) 257, 844
FbaI TGATCA 1 cut(s) 571
Fnu4HI GCNGC 5 cut(s) 27, 688, 727, 730, 916
FokI GGATG 4 cut(s) 463, 581, 686, 845
Fsp4HI GCNGC 5 cut(s) 27, 688, 727, 730, 916
FspBI CTAG 3 cut(s) 123, 129, 375
GlaI GCGC 1 cut(s) 48
GluI GCNGC 5 cut(s) 27, 688, 727, 730, 916
GsaI CCCAGC 1 cut(s) 625
HaeII RGCGCY 1 cut(s) 50
HaeIII GGCC 3 cut(s) 259, 304, 918
HapII CCGG 2 cut(s) 44, 50
HhaI GCGC 1 cut(s) 49
Hin1I GRCGYC 1 cut(s) 47
Hin1II CATG 7 cut(s) 140, 479, 639, 668, 673, 888, 977
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HindIII AAGCTT 1 cut(s) 457
HinfI GANTC 6 cut(s) 416, 500, 552, 893, 935, 946
HpaII CCGG 2 cut(s) 44, 50
HphI GGTGA 1 cut(s) 763
Hpy188I TCNGA 6 cut(s) 421, 450, 495, 703, 795, 940
Hpy188III TCNNGA 4 cut(s) 370, 476, 556, 764
HpyAV CCTTC 3 cut(s) 89, 638, 749
HpyCH4III ACNGT 1 cut(s) 473
HpyCH4V TGCA 5 cut(s) 94, 333, 664, 690, 729
HpyF3I CTNAG 5 cut(s) 194, 306, 425, 626, 758
Hsp92I GRCGYC 1 cut(s) 47
Hsp92II CATG 7 cut(s) 140, 479, 639, 668, 673, 888, 977
HspAI GCGC 1 cut(s) 47
KasI GGCGCC 1 cut(s) 46
KroI GCCGGC 1 cut(s) 49
KroNI GCCGGC 1 cut(s) 51
Ksp22I TGATCA 1 cut(s) 571
Kzo9I GATC 6 cut(s) 8, 97, 490, 543, 571, 790
LmnI GCTCC 3 cut(s) 141, 295, 583
Lsp1109I GCAGC 3 cut(s) 674, 713, 741
MaeI CTAG 3 cut(s) 123, 129, 375
MaeIII GTNAC 1 cut(s) 612
MalI GATC 6 cut(s) 10, 99, 492, 545, 573, 792
MboI GATC 6 cut(s) 8, 97, 490, 543, 571, 790
MboII GAAGA 9 cut(s) 43, 67, 70, 144, 147, 210, 719, 725, 878
MflI RGATCY 1 cut(s) 97
MluCI AATT 5 cut(s) 226, 322, 365, 512, 538
Mly113I GGCGCC 1 cut(s) 47
MlyI GAGTC 1 cut(s) 902
MmeI TCCRAC 2 cut(s) 546, 615
Mph1103I ATGCAT 1 cut(s) 666
MreI CGCCGGCG 1 cut(s) 49
MroNI GCCGGC 1 cut(s) 49
MroXI GAANNNNTTC 2 cut(s) 444, 870
MseI TTAA 4 cut(s) 230, 800, 861, 979
MspA1I CMGCKG 1 cut(s) 625
MspI CCGG 2 cut(s) 44, 50
MspR9I CCNGG 4 cut(s) 256, 583, 814, 843
Mva1269I GAATGC 1 cut(s) 586
MvaI CCWGG 4 cut(s) 256, 583, 814, 843
NaeI GCCGGC 1 cut(s) 51
NarI GGCGCC 1 cut(s) 47
NcoI CCATGG 1 cut(s) 136
NdeII GATC 6 cut(s) 8, 97, 490, 543, 571, 790
NgoMIV GCCGGC 1 cut(s) 49
NlaIII CATG 7 cut(s) 140, 479, 639, 668, 673, 888, 977
NlaIV GGNNCC 2 cut(s) 48, 579
NmeAIII GCCGAG 1 cut(s) 916
NmuCI GTSAC 1 cut(s) 612
NsiI ATGCAT 1 cut(s) 666
NspV TTCGAA 1 cut(s) 82
PagI TCATGA 1 cut(s) 475
PceI AGGCCT 2 cut(s) 259, 304
PctI GAATGC 1 cut(s) 586
PdiI GCCGGC 1 cut(s) 51
PdmI GAANNNNTTC 2 cut(s) 444, 870
PfeI GAWTC 5 cut(s) 416, 500, 552, 935, 946
PkrI GCNGC 5 cut(s) 28, 689, 728, 731, 917
Ple19I CGATCG 1 cut(s) 11
PleI GAGTC 1 cut(s) 901
PluTI GGCGCC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 901
Psp6I CCWGG 4 cut(s) 254, 581, 812, 841
PspFI CCCAGC 1 cut(s) 621
PspGI CCWGG 4 cut(s) 254, 581, 812, 841
PspN4I GGNNCC 2 cut(s) 48, 579
PstI CTGCAG 2 cut(s) 692, 731
PstNI CAGNNNCTG 1 cut(s) 437
PsuI RGATCY 1 cut(s) 97
PvuI CGATCG 1 cut(s) 11
PvuII CAGCTG 1 cut(s) 625
RsaI GTAC 1 cut(s) 127
RsaNI GTAC 1 cut(s) 126
SaqAI TTAA 4 cut(s) 230, 800, 861, 979
SatI GCNGC 5 cut(s) 27, 688, 727, 730, 916
Sau3AI GATC 6 cut(s) 8, 97, 490, 543, 571, 790
ScaI AGTACT 1 cut(s) 127
SchI GAGTC 1 cut(s) 902
ScrFI CCNGG 4 cut(s) 256, 583, 814, 843
SetI ASST 8 cut(s) 190, 200, 292, 445, 461, 627, 741, 969
SfcI CTRYAG 2 cut(s) 688, 727
SfoI GGCGCC 1 cut(s) 48
SfuI TTCGAA 1 cut(s) 82
SgrAI CRCCGGYG 1 cut(s) 49
SpeI ACTAGT 2 cut(s) 122, 374
Sse9I AATT 5 cut(s) 226, 322, 365, 512, 538
SseBI AGGCCT 2 cut(s) 259, 304
SsiI CCGC 4 cut(s) 26, 250, 851, 915
SspDI GGCGCC 1 cut(s) 46
SspMI CTAG 3 cut(s) 123, 129, 375
StuI AGGCCT 2 cut(s) 259, 304
StyD4I CCNGG 4 cut(s) 254, 581, 812, 841
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 1 cut(s) 473
TaqI TCGA 4 cut(s) 82, 174, 542, 550
TasI AATT 5 cut(s) 226, 322, 365, 512, 538
TatI WGTACW 1 cut(s) 125
TauI GCSGC 2 cut(s) 29, 918
TfiI GAWTC 5 cut(s) 416, 500, 552, 935, 946
Tru1I TTAA 4 cut(s) 230, 800, 861, 979
Tru9I TTAA 4 cut(s) 230, 800, 861, 979
TscAI CASTG 2 cut(s) 438, 910
TseFI GTSAC 1 cut(s) 612
TseI GCWGC 3 cut(s) 687, 726, 729
Tsp45I GTSAC 1 cut(s) 612
TspDTI ATGAA 4 cut(s) 17, 492, 690, 852
TspRI CASTG 2 cut(s) 438, 910
XapI RAATTY 1 cut(s) 365
XmnI GAANNNNTTC 2 cut(s) 444, 870
XspI CTAG 3 cut(s) 123, 129, 375
ZrmI AGTACT 1 cut(s) 127
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.