FvH4_1g01450

ATPase 8, plasma

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0011636
Physical Location & Seq
Forward (+)
735002 .. 742782
7781 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g01450.t2

Sequence Viewer

Length: 2847 bp
ATGGCCGATATCTCCTTGGAGGACGTGAAGAACGAGAATGTCGACCTTGAGAAAATCCCGGTGGAGGAAGTGTTCGAGCAGCTGAAATGCTCGAAACAAGGGTTGAGCACAGATGAGGGAACGAAAAGGCTTCAGGTTTTCGGCAAGAATAAGCTTGAGGAAAAGAAGGAGAGCAAGGTTCTCAAGTTCCTAGGGTTCATGTGGAATCCCTTATCATGGGTGATGGAGCTGGCTGCAATCATGGCCATTGCTTTGGCCAATGGAGGGGGAAAGCCTACGGATTGGCCGGACTTCGTCGGTATTGTGGTGCTTCTGTTCATCAACTCAACTATCTCATTTATCGAAGAGAACAATGCTGGCAATGCTGCCGCAGCTCTTATGGCTAGTCTTGCCCCCAAGACCAAGGTGTTAAGGGATGGAAAATGGGGTGAGAAAGAGGCAGAAATTTTGGTACCCGGGGATATCATCAGCATTAAGTTGGGAGACATAGTCCCAGCTGATGCTCGTCTCCTGGAAGGTGATCCTTTAAAGATTGACCAGGCTGCACTCACTGGTGAATCCCTACCGGTTACCAAAAACCCCGGCGATGAGGTCTTCTCTGGATCTACTTGCAAGCAGGGTGAGATTGAGGCTGTTGTTATCGCAACTGGAGTCCACACTTTCTTTGGCAAGGCTGCACACTTGGTGGATAGCACCAACCAAGTTGGTCACTTCCAAAAGGTGTTGACATCTATTGGTAACTTCTGCATCTGCTCCATTGGAGTTGGAATGGTGATTGAGATATTGGTGATGTACCCCATTCAGCACAGGGCATATAGAGATGGTATTGACAATCTGTTGGTGCTCCTAATCGGAGGTATCCCCATTGCCATGCCAACGGTGTTATCCGTGACCATGGCTATTGGGTCTCACCGCTTGTCACAGCAGGGTGCCATCACCAAGAGGATGACAGCCATTGAAGAAATGGCAGGAATGGATGTACTTTGCAGTGACAAGACCGGAACTCTCACCCTCAACAAGCTTACTGTCGACAAGAGTCTGATTGAGGTGTTCCCTAAGAACTTGGACAAAGATGGAGTGGTCTTGCTCTCTGCCAGGGCTTCCAGGATAGAAAACCAGGACGCAATCGATGCTTCAATTGTTGGGATGCTGGGTGACCCAAAGGAGGCAAGAGCAGGGATCACTGAGGTACATTTCCTGCCCTTCAACCCTGTCGAAAAGCGCACAGCAATCACCTACATTGATAGCAATGGGGATTGGCACAGATGCAGCAAGGGTGCTCCTGAGCAAATCATTGACCTATGTGACCTTAAGGGAGAAGCGAGGAAGAAGGCACACGAAGTCATTGACAACTATGCTGACCGTGGTCTTCGTTCATTGGGTGTGGCACGACAGACAGTATCAGAGAAGACCAAGGAAAGTGCTGGTGATCCATGGGAGTTTGTTGGTCTATTGCCTCTCTTTGATCCTCCAAGGCATGACAGTGCCGAGACTATCCGCCGTGCTCTTGAGCTTGGTGTCAATGTAAAGATGATCACTGGTGACCAACTTGCCATTGGAAAGGAGACCGGCCGAAGGCTTGGCATGGGCACCAACATGTACCCTTCATCTACCCTTCTTGGCCAAAGCTTGGACGAGTCCATTGCTACAATTCCTGTTGATGAGCTCATTGAAAAGGCTGATGGATTTGCTGGAGTCTTCCCTGAGCACAAGTATGAGATTGTCAAGAAGCTGCAGGAGAGGAAACACATCTGTGGTATGACCGGAGATGGTGTGAATGACGCCCCGGCTCTCAAGAGAGCAGACATTGGCATTGCAGTGGATGATGCAACTGATGCAGCCAGGAGTGCCTCAGACATTGTACTGACTGAGCCAGGCCTGAGTGTTATAGTGAGCGCCGTGTTGACAAGCAGAGCCATCTTCCAGAGGATGAAGAACTACACCATTTATGCTGTTTCCATCACAATCCGTATTGTGGTGGGATTCATGCTCCTTGCTCTCATCTGGAAGTTTGACTTCTCACCATTCATGGTCTTGGTCATTGCAATCTTGAATGACGGCACCATCATGACCATCTCTAAGGATAGAGTGAAGCCATCTCCTCTGCCCGACTCCTGGAAGCTCAAGGAAATCTTTGCCACTGGAATTGTCCTCGGAACTTACATGGCTGTCATGACTGTGGTCTTCTTCTGGATTGCACACAGCACTCAGTTCTTCTCTAGAGTATTTGGTGTGCGACATATTGGGGACGACGCGGCGCAGCTTACCTCTGCTGTCTACCTCCAAGTGAGCATTCTCAGTCAAGCGCTCATCTTCGTGACCCGATCAAGGAGCTTCTCCTTTCTTGAACGCCCCGGTACCATGCTCATGGGTGCCTTTCTTGCAGCGCAGTTGGTGGCTACCCTCATTGCTGTGTATTGTAGCTGGGGCTTTGCTAGAATCGATGGTGTCGGATGGGGATGGGCAGGTGTTATCTGGATCTACAGCATTGTCACCTACTTCCCTCTCGACATCTTCAAGTTCATAATCCGTTACGCATTGAGTGGCCATGCTTGGGATAGTGTTGTACAGCACAAGACTGCCTTCTCAACAAACAATGACTATGGAAAGGCTGAGAGAGAGGCACAGTGGGCAGCAACTCAACGAACACTCTCTGGTCTCCAGTCTCCTCCGGAATTGTTCCACGGCAACCACCATGGGGATGAGCAATCGATAGCTGAGCAAGCCAAGAGACGAGCAGAAGTTGCTAGGCTGAGGGAGCTCCACACCTTAAAGGGGCACGTCCAATCTGTGGTCAAGCTGAAGGGGCTTGATATCGAAACTATCCAGCAGAACTACACCGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

949

Amino Acids

103.77

Weight (kDa)

5.93

Isoelectric Point (pI)

33.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 18 - 81 1.5e-15 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 130 - 229 6.5e-31 P-type ATPase actuator domain
Hydrolase PF00702 325 - 602 3.6e-14 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 387 - 493 5.5e-06 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011636)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07560 AT3G42640
fragaria_vesca FvH4_1g01450 FvH4_1g01450
malus_domestica MD02G1011600.v1.1
prunus_persica Prupe.7G258000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0086371
rosa_laevigata RLG00000015753
rosa_multiflora Rmu_co8318751.1_g000001 Rmu_sc0003572.1_g000001 Rmu_sc0005127.1_g000010
rosa_roxburghii Rroxscaffold_2G00154660
rosa_rugosa Rorug01G0466900 Rorug01G0467000
rosa_samantha Rh2AG017100 Rh2BG016900 Rh2CG017900 Rh2DG018600
rosa_wichuraiana Rw2G001450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2456
Acc36I ACCTGC 1 cut(s) 2456
Acc65I GGTACC 2 cut(s) 451, 2357
AccB1I GGYRCC 6 cut(s) 451, 929, 1589, 2060, 2357, 2372
AccB7I CCANNNNNTGG 2 cut(s) 1630, 2791
AccI GTMKAC 3 cut(s) 42, 1029, 2277
AccII CGCG 1 cut(s) 2255
AccIII TCCGGA 1 cut(s) 2671
AciI CCGC 4 cut(s) 369, 913, 1498, 2255
AclWI GGATC 6 cut(s) 515, 610, 1187, 1424, 1460, 2486
AcoI YGGCCR 7 cut(s) 3, 243, 255, 284, 1570, 1621, 2545
AcsI RAATTY 1 cut(s) 444
AcuI CTGAAG 2 cut(s) 116, 2822
AcyI GRCGYC 1 cut(s) 1782
AdeI CACNNNGTG 1 cut(s) 685
AfaI GTAC 8 cut(s) 453, 794, 981, 1191, 1601, 1863, 2359, 2568
AfeI AGCGCT 1 cut(s) 2307
AflII CTTAAG 1 cut(s) 1310
AflIII ACRYGT 1 cut(s) 1596
AgeI ACCGGT 1 cut(s) 565
AgsI TTSAA 7 cut(s) 959, 1137, 1207, 1673, 2053, 2348, 2518
AjiI CACGTC 2 cut(s) 25, 2782
AjnI CCWGG 8 cut(s) 510, 537, 1094, 1103, 1116, 1841, 1873, 2114
AjuI GAANNNNNNNTTGG 2 cut(s) 2277, 2309
AleI CACNNNNGTG 1 cut(s) 1752
AloI GAACNNNNNNTCC 2 cut(s) 56, 88
Alw21I GWGCWC 7 cut(s) 110, 846, 1282, 1507, 1668, 1710, 2763
Alw26I GTCTC 8 cut(s) 477, 512, 912, 1484, 1559, 2663, 2670, 2725
AlwI GGATC 6 cut(s) 515, 610, 1187, 1424, 1460, 2486
Ama87I CYCGRG 1 cut(s) 455
Aor13HI TCCGGA 1 cut(s) 2671
Aor51HI AGCGCT 1 cut(s) 2307
AoxI GGCC 8 cut(s) 3, 243, 255, 284, 1570, 1621, 1876, 2545
ApoI RAATTY 1 cut(s) 444
AsiGI ACCGGT 1 cut(s) 565
Asp718I GGTACC 2 cut(s) 451, 2357
AspA2I CCTAGG 1 cut(s) 190
AspLEI GCGC 5 cut(s) 1224, 1898, 2260, 2308, 2389
AsuC2I CCSGG 6 cut(s) 59, 456, 457, 582, 1787, 2355
AvaI CYCGRG 1 cut(s) 455
AvrII CCTAGG 1 cut(s) 190
BaeGI GKGCMC 2 cut(s) 1592, 2781
BalI TGGCCA 4 cut(s) 245, 257, 1623, 2547
BanI GGYRCC 6 cut(s) 451, 929, 1589, 2060, 2357, 2372
BanII GRGCYC 2 cut(s) 1668, 2763
BbsI GAAGAC 5 cut(s) 586, 1361, 1415, 1690, 2176
Bbv12I GWGCWC 7 cut(s) 110, 846, 1282, 1507, 1668, 1710, 2763
BbvCI CCTCAGC 1 cut(s) 2753
BceAI ACGGC 4 cut(s) 1485, 1883, 2074, 2701
BcgI CGANNNNNNTGC 2 cut(s) 1625, 1659
BciT130I CCWGG 8 cut(s) 512, 539, 1096, 1105, 1118, 1843, 1875, 2116
BciVI GTATCC 1 cut(s) 869
BclI TGATCA 1 cut(s) 1533
BcnI CCSGG 6 cut(s) 59, 456, 457, 582, 1787, 2355
BcoDI GTCTC 8 cut(s) 477, 512, 912, 1484, 1559, 2663, 2670, 2725
BfaI CTAG 6 cut(s) 191, 384, 2220, 2436, 2748, 2845
BfmI CTRYAG 2 cut(s) 1733, 2482
BfoI RGCGCY 2 cut(s) 1899, 2309
BfrI CTTAAG 1 cut(s) 1310
BfuAI ACCTGC 1 cut(s) 2456
BfuI GTATCC 1 cut(s) 869
BlnI CCTAGG 1 cut(s) 190
BlpI GCTNAGC 1 cut(s) 2718
BmeT110I CYCGRG 1 cut(s) 455
BmgBI CACGTC 2 cut(s) 25, 2782
BmiI GGNNCC 6 cut(s) 453, 931, 1591, 2062, 2359, 2374
BmsI GCATC 7 cut(s) 490, 756, 1120, 1137, 1256, 1816, 1825
BoxI GACNNNNGTC 3 cut(s) 1035, 1365, 2180
BpiI GAAGAC 5 cut(s) 586, 1361, 1415, 1690, 2176
BplI GAGNNNNNCTC 2 cut(s) 581, 613
BpmI CTGGAG 3 cut(s) 669, 1713, 2645
Bpu10I CCTNAGC 3 cut(s) 1284, 1704, 2753
Bpu1102I GCTNAGC 1 cut(s) 2718
BpuEI CTTGAG 6 cut(s) 68, 167, 176, 1529, 1778, 2108
BpuMI CCSGG 6 cut(s) 59, 456, 457, 582, 1787, 2355
Bsa29I ATCGAT 3 cut(s) 1128, 2442, 2711
BsaHI GRCGYC 1 cut(s) 1782
BsaI GGTCTC 3 cut(s) 912, 1559, 2663
BsaWI WCCGGW 4 cut(s) 565, 998, 1763, 2671
BsaXI ACNNNNNCTCC 6 cut(s) 56, 86, 474, 504, 1758, 1788
Bse118I RCCGGY 2 cut(s) 565, 1568
Bse1I ACTGG 5 cut(s) 556, 652, 1543, 2146, 2662
Bse3DI GCAATG 8 cut(s) 246, 367, 864, 1255, 1641, 1812, 2040, 2406
BseAI TCCGGA 1 cut(s) 2671
BseBI CCWGG 8 cut(s) 512, 539, 1096, 1105, 1118, 1843, 1875, 2116
BseCI ATCGAT 3 cut(s) 1128, 2442, 2711
BseGI GGATG 9 cut(s) 421, 951, 982, 1152, 1828, 1935, 2459, 2465, 2707
BseMI GCAATG 8 cut(s) 246, 367, 864, 1255, 1641, 1812, 2040, 2406
BseNI ACTGG 5 cut(s) 556, 652, 1543, 2146, 2662
BseRI GAGGAG 2 cut(s) 2091, 2658
BseSI GKGCMC 2 cut(s) 1592, 2781
BseX3I CGGCCG 1 cut(s) 1570
BseYI CCCAGC 3 cut(s) 493, 1150, 2424
BsgI GTGCAG 2 cut(s) 528, 660
Bsh1236I CGCG 1 cut(s) 2255
Bsh1285I CGRYCG 1 cut(s) 1573
BshFI GGCC 8 cut(s) 5, 245, 257, 286, 1572, 1623, 1878, 2547
BshNI GGYRCC 6 cut(s) 451, 929, 1589, 2060, 2357, 2372
BshTI ACCGGT 1 cut(s) 565
BshVI ATCGAT 3 cut(s) 1128, 2442, 2711
BsiEI CGRYCG 1 cut(s) 1573
BsiHKAI GWGCWC 7 cut(s) 110, 846, 1282, 1507, 1668, 1710, 2763
BsiHKCI CYCGRG 1 cut(s) 455
BslFI GGGAC 2 cut(s) 476, 2261
BsmAI GTCTC 8 cut(s) 477, 512, 912, 1484, 1559, 2663, 2670, 2725
BsmBI CGTCTC 2 cut(s) 512, 2725
BsmFI GGGAC 2 cut(s) 476, 2261
BsmI GAATGC 1 cut(s) 2292
BsnI GGCC 8 cut(s) 5, 245, 257, 286, 1572, 1623, 1878, 2547
Bso31I GGTCTC 3 cut(s) 912, 1559, 2663
BsoBI CYCGRG 1 cut(s) 455
Bsp1286I GDGCHC 9 cut(s) 110, 846, 1282, 1507, 1592, 1668, 1710, 2763, 2781
Bsp13I TCCGGA 1 cut(s) 2671
Bsp1407I TGTACA 1 cut(s) 2566
Bsp143I GATC 8 cut(s) 520, 602, 1179, 1429, 1465, 1533, 2324, 2478
Bsp1720I GCTNAGC 1 cut(s) 2718
Bsp19I CCATGG 3 cut(s) 894, 1433, 2695
BspACI CCGC 4 cut(s) 369, 913, 1498, 2255
BspANI GGCC 8 cut(s) 5, 245, 257, 286, 1572, 1623, 1878, 2547
BspDI ATCGAT 3 cut(s) 1128, 2442, 2711
BspEI TCCGGA 1 cut(s) 2671
BspFNI CGCG 1 cut(s) 2255
BspHI TCATGA 2 cut(s) 2067, 2172
BspLI GGNNCC 6 cut(s) 453, 931, 1591, 2062, 2359, 2374
BspMAI CTGCAG 1 cut(s) 1737
BspMI ACCTGC 1 cut(s) 2456
BspPI GGATC 6 cut(s) 515, 610, 1187, 1424, 1460, 2486
BspT107I GGYRCC 6 cut(s) 451, 929, 1589, 2060, 2357, 2372
BspTI CTTAAG 1 cut(s) 1310
BspTNI GGTCTC 3 cut(s) 912, 1559, 2663
BsrDI GCAATG 8 cut(s) 246, 367, 864, 1255, 1641, 1812, 2040, 2406
BsrFI RCCGGY 2 cut(s) 565, 1568
BsrGI TGTACA 1 cut(s) 2566
BsrI ACTGG 5 cut(s) 556, 652, 1543, 2146, 2662
BssAI RCCGGY 2 cut(s) 565, 1568
BssMI GATC 8 cut(s) 520, 602, 1179, 1429, 1465, 1533, 2324, 2478
BssNI GRCGYC 1 cut(s) 1782
BssT1I CCWWGG 8 cut(s) 15, 190, 402, 894, 1413, 1433, 1472, 2695
Bst2UI CCWGG 8 cut(s) 512, 539, 1096, 1105, 1118, 1843, 1875, 2116
Bst4CI ACNGT 8 cut(s) 880, 1027, 1364, 1399, 1484, 2179, 2628, 2842
Bst6I CTCTTC 1 cut(s) 339
BstACI GRCGYC 1 cut(s) 1782
BstAFI CTTAAG 1 cut(s) 1310
BstAPI GCANNNNNTGC 3 cut(s) 1130, 1835, 2744
BstAUI TGTACA 1 cut(s) 2566
BstC8I GCNNGC 4 cut(s) 231, 358, 614, 2724
BstDSI CCRYGG 5 cut(s) 894, 1363, 1433, 2683, 2695
BstEII GGTNACC 3 cut(s) 568, 1154, 1541
BstF5I GGATG 9 cut(s) 421, 951, 982, 1152, 1828, 1935, 2459, 2465, 2707
BstFNI CGCG 1 cut(s) 2255
BstH2I RGCGCY 2 cut(s) 1899, 2309
BstHHI GCGC 5 cut(s) 1224, 1898, 2260, 2308, 2389
BstKTI GATC 8 cut(s) 523, 605, 1182, 1432, 1468, 1536, 2327, 2481
BstMAI GTCTC 8 cut(s) 477, 512, 912, 1484, 1559, 2663, 2670, 2725
BstMBI GATC 8 cut(s) 520, 602, 1179, 1429, 1465, 1533, 2324, 2478
BstMCI CGRYCG 1 cut(s) 1573
BstNI CCWGG 8 cut(s) 512, 539, 1096, 1105, 1118, 1843, 1875, 2116
BstNSI RCATGY 1 cut(s) 1600
BstPAI GACNNNNGTC 3 cut(s) 1035, 1365, 2180
BstPI GGTNACC 3 cut(s) 568, 1154, 1541
BstSFI CTRYAG 2 cut(s) 1733, 2482
BstSLI GKGCMC 2 cut(s) 1592, 2781
BstUI CGCG 1 cut(s) 2255
BstV2I GAAGAC 5 cut(s) 586, 1361, 1415, 1690, 2176
BstX2I RGATCY 2 cut(s) 602, 2478
BstXI CCANNNNNNTGG 2 cut(s) 253, 2368
BstYI RGATCY 2 cut(s) 602, 2478
BstZI CGGCCG 1 cut(s) 1570
Bsu15I ATCGAT 3 cut(s) 1128, 2442, 2711
BsuI GTATCC 1 cut(s) 869
BsuRI GGCC 8 cut(s) 5, 245, 257, 286, 1572, 1623, 1878, 2547
BsuTUI ATCGAT 3 cut(s) 1128, 2442, 2711
BtgI CCRYGG 5 cut(s) 894, 1363, 1433, 2683, 2695
BtgZI GCGATG 1 cut(s) 600
BtrI CACGTC 2 cut(s) 25, 2782
BtsCI GGATG 9 cut(s) 421, 951, 982, 1152, 1828, 1935, 2459, 2465, 2707
BtsI GCAGTG 2 cut(s) 994, 1824
BtsIMutI CAGTG 8 cut(s) 549, 994, 1182, 1489, 1536, 1824, 2139, 2633
BveI ACCTGC 1 cut(s) 2456
Cac8I GCNNGC 4 cut(s) 231, 358, 614, 2724
CciI TCATGA 2 cut(s) 2067, 2172
CfoI GCGC 5 cut(s) 1224, 1898, 2260, 2308, 2389
Cfr10I RCCGGY 2 cut(s) 565, 1568
Cfr9I CCCGGG 1 cut(s) 455
ClaI ATCGAT 3 cut(s) 1128, 2442, 2711
CseI GACGC 3 cut(s) 1130, 1790, 2261
Csp6I GTAC 8 cut(s) 452, 793, 980, 1190, 1600, 1862, 2358, 2567
CspAI ACCGGT 1 cut(s) 565
CspCI CAANNNNNGTGG 2 cut(s) 2128, 2163
CviQI GTAC 8 cut(s) 452, 793, 980, 1190, 1600, 1862, 2358, 2567
DpnI GATC 8 cut(s) 522, 604, 1181, 1431, 1467, 1535, 2326, 2480
DpnII GATC 8 cut(s) 520, 602, 1179, 1429, 1465, 1533, 2324, 2478
DraI TTTAAA 1 cut(s) 528
DraIII CACNNNGTG 1 cut(s) 685
EaeI YGGCCR 7 cut(s) 3, 243, 255, 284, 1570, 1621, 2545
EagI CGGCCG 1 cut(s) 1570
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
EciI GGCGGA 1 cut(s) 1487
Ecl136II GAGCTC 2 cut(s) 1666, 2761
EclXI CGGCCG 1 cut(s) 1570
Eco130I CCWWGG 8 cut(s) 15, 190, 402, 894, 1413, 1433, 1472, 2695
Eco147I AGGCCT 1 cut(s) 1878
Eco24I GRGCYC 2 cut(s) 1668, 2763
Eco31I GGTCTC 3 cut(s) 912, 1559, 2663
Eco32I GATATC 3 cut(s) 10, 463, 2815
Eco47III AGCGCT 1 cut(s) 2307
Eco52I CGGCCG 1 cut(s) 1570
Eco53kI GAGCTC 2 cut(s) 1666, 2761
Eco57I CTGAAG 2 cut(s) 116, 2822
Eco88I CYCGRG 1 cut(s) 455
Eco91I GGTNACC 3 cut(s) 568, 1154, 1541
EcoICRI GAGCTC 2 cut(s) 1666, 2761
EcoO65I GGTNACC 3 cut(s) 568, 1154, 1541
EcoRII CCWGG 8 cut(s) 510, 537, 1094, 1103, 1116, 1841, 1873, 2114
EcoRV GATATC 3 cut(s) 10, 463, 2815
EcoT14I CCWWGG 8 cut(s) 15, 190, 402, 894, 1413, 1433, 1472, 2695
EcoT38I GRGCYC 2 cut(s) 1668, 2763
ErhI CCWWGG 8 cut(s) 15, 190, 402, 894, 1413, 1433, 1472, 2695
Esp3I CGTCTC 2 cut(s) 512, 2725
FalI AAGNNNNNCTT 6 cut(s) 2000, 2032, 2117, 2149, 2567, 2599
FaqI GGGAC 2 cut(s) 476, 2261
FbaI TGATCA 1 cut(s) 1533
FblI GTMKAC 3 cut(s) 42, 1029, 2277
FokI GGATG 9 cut(s) 428, 958, 989, 1159, 1835, 1942, 2466, 2472, 2714
FriOI GRGCYC 2 cut(s) 1668, 2763
FspBI CTAG 6 cut(s) 191, 384, 2220, 2436, 2748, 2845
GlaI GCGC 5 cut(s) 1223, 1897, 2259, 2307, 2388
GsaI CCCAGC 3 cut(s) 497, 1154, 2428
GsuI CTGGAG 3 cut(s) 669, 1713, 2645
HaeII RGCGCY 2 cut(s) 1899, 2309
HaeIII GGCC 8 cut(s) 5, 245, 257, 286, 1572, 1623, 1878, 2547
HgaI GACGC 3 cut(s) 1130, 1790, 2261
HhaI GCGC 5 cut(s) 1224, 1898, 2260, 2308, 2389
Hin1I GRCGYC 1 cut(s) 1782
Hin6I GCGC 5 cut(s) 1222, 1896, 2258, 2306, 2387
HinP1I GCGC 5 cut(s) 1222, 1896, 2258, 2306, 2387
HincII GTYRAC 4 cut(s) 43, 726, 1030, 1905
HindII GTYRAC 4 cut(s) 43, 726, 1030, 1905
HindIII AAGCTT 3 cut(s) 152, 1019, 1627
HinfI GANTC 9 cut(s) 205, 557, 651, 1036, 1637, 1695, 1983, 2111, 2439
Hpy166II GTNNAC 6 cut(s) 43, 655, 726, 1030, 1905, 2278
Hpy188I TCNGA 6 cut(s) 854, 1041, 1405, 1855, 2156, 2453
Hpy8I GTNNAC 6 cut(s) 43, 655, 726, 1030, 1905, 2278
Hpy99I CGWCG 2 cut(s) 299, 2255
HpyAV CCTTC 9 cut(s) 160, 509, 1213, 1324, 1569, 1614, 1625, 2593, 2797
HpyCH4III ACNGT 8 cut(s) 880, 1027, 1364, 1399, 1484, 2179, 2628, 2842
HpyCH4IV ACGT 2 cut(s) 24, 2781
HpySE526I ACGT 2 cut(s) 24, 2781
Hsp92I GRCGYC 1 cut(s) 1782
HspAI GCGC 5 cut(s) 1222, 1896, 2258, 2306, 2387
Kpn2I TCCGGA 1 cut(s) 2671
KpnI GGTACC 2 cut(s) 455, 2361
Ksp22I TGATCA 1 cut(s) 1533
Kzo9I GATC 8 cut(s) 520, 602, 1179, 1429, 1465, 1533, 2324, 2478
LmnI GCTCC 8 cut(s) 226, 758, 849, 1285, 1995, 2331, 2758, 2766
LweI GCATC 7 cut(s) 490, 756, 1120, 1137, 1256, 1816, 1825
MaeI CTAG 6 cut(s) 191, 384, 2220, 2436, 2748, 2845
MaeII ACGT 2 cut(s) 24, 2781
MalI GATC 8 cut(s) 522, 604, 1181, 1431, 1467, 1535, 2326, 2480
MboI GATC 8 cut(s) 520, 602, 1179, 1429, 1465, 1533, 2324, 2478
MfeI CAATTG 1 cut(s) 1137
MflI RGATCY 2 cut(s) 602, 2478
MhlI GDGCHC 9 cut(s) 110, 846, 1282, 1507, 1592, 1668, 1710, 2763, 2781
MlsI TGGCCA 4 cut(s) 245, 257, 1623, 2547
MluCI AATT 5 cut(s) 444, 1137, 1650, 2145, 2675
MluNI TGGCCA 4 cut(s) 245, 257, 1623, 2547
MlyI GAGTC 5 cut(s) 660, 1045, 1646, 1704, 2105
MmeI TCCRAC 2 cut(s) 745, 2431
Mox20I TGGCCA 4 cut(s) 245, 257, 1623, 2547
MroI TCCGGA 1 cut(s) 2671
MscI TGGCCA 4 cut(s) 245, 257, 1623, 2547
MseI TTAA 5 cut(s) 410, 474, 527, 1311, 2771
Msp20I TGGCCA 4 cut(s) 245, 257, 1623, 2547
MspA1I CMGCKG 2 cut(s) 82, 497
MspCI CTTAAG 1 cut(s) 1310
MunI CAATTG 1 cut(s) 1137
Mva1269I GAATGC 1 cut(s) 2292
MvaI CCWGG 8 cut(s) 512, 539, 1096, 1105, 1118, 1843, 1875, 2116
MvnI CGCG 1 cut(s) 2255
NciI CCSGG 6 cut(s) 59, 456, 457, 582, 1787, 2355
NcoI CCATGG 3 cut(s) 894, 1433, 2695
NdeII GATC 8 cut(s) 520, 602, 1179, 1429, 1465, 1533, 2324, 2478
NlaIV GGNNCC 6 cut(s) 453, 931, 1591, 2062, 2359, 2374
NmeAIII GCCGAG 1 cut(s) 1513
NmuCI GTSAC 9 cut(s) 707, 889, 918, 989, 1154, 1304, 1541, 2317, 2491
NspI RCATGY 1 cut(s) 1600
OliI CACNNNNGTG 1 cut(s) 1752
PagI TCATGA 2 cut(s) 2067, 2172
PaqCI CACCTGC 1 cut(s) 2456
PceI AGGCCT 1 cut(s) 1878
PciI ACATGT 1 cut(s) 1596
PcsI WCGNNNNNNNCGW 2 cut(s) 30, 39
PctI GAATGC 1 cut(s) 2292
PfeI GAWTC 4 cut(s) 205, 557, 1983, 2439
PflFI GACNNNGTC 2 cut(s) 293, 488
PflMI CCANNNNNTGG 2 cut(s) 1630, 2791
PfoI TCCNGGA 3 cut(s) 510, 1103, 2114
PinAI ACCGGT 1 cut(s) 565
PleI GAGTC 5 cut(s) 659, 1044, 1645, 1703, 2105
PpsI GAGTC 5 cut(s) 659, 1044, 1645, 1703, 2105
PscI ACATGT 1 cut(s) 1596
PshAI GACNNNNGTC 3 cut(s) 1035, 1365, 2180
Psp124BI GAGCTC 2 cut(s) 1668, 2763
Psp6I CCWGG 8 cut(s) 510, 537, 1094, 1103, 1116, 1841, 1873, 2114
PspEI GGTNACC 3 cut(s) 568, 1154, 1541
PspFI CCCAGC 3 cut(s) 493, 1150, 2424
PspGI CCWGG 8 cut(s) 510, 537, 1094, 1103, 1116, 1841, 1873, 2114
PspN4I GGNNCC 6 cut(s) 453, 931, 1591, 2062, 2359, 2374
PstI CTGCAG 1 cut(s) 1737
PsuI RGATCY 2 cut(s) 602, 2478
PsyI GACNNNGTC 2 cut(s) 293, 488
PvuII CAGCTG 2 cut(s) 82, 497
RsaI GTAC 8 cut(s) 453, 794, 981, 1191, 1601, 1863, 2359, 2568
RsaNI GTAC 8 cut(s) 452, 793, 980, 1190, 1600, 1862, 2358, 2567
SacI GAGCTC 2 cut(s) 1668, 2763
SalI GTCGAC 2 cut(s) 41, 1028
SaqAI TTAA 5 cut(s) 410, 474, 527, 1311, 2771
Sau3AI GATC 8 cut(s) 520, 602, 1179, 1429, 1465, 1533, 2324, 2478
SchI GAGTC 5 cut(s) 660, 1045, 1646, 1704, 2105
SduI GDGCHC 9 cut(s) 110, 846, 1282, 1507, 1592, 1668, 1710, 2763, 2781
SfaNI GCATC 7 cut(s) 490, 756, 1120, 1137, 1256, 1816, 1825
SfcI CTRYAG 2 cut(s) 1733, 2482
SmaI CCCGGG 1 cut(s) 457
SmlI CTYRAG 7 cut(s) 47, 155, 182, 1310, 1508, 1793, 2123
SmoI CTYRAG 7 cut(s) 47, 155, 182, 1310, 1508, 1793, 2123
Sse9I AATT 5 cut(s) 444, 1137, 1650, 2145, 2675
SseBI AGGCCT 1 cut(s) 1878
SsiI CCGC 4 cut(s) 369, 913, 1498, 2255
SspMI CTAG 6 cut(s) 191, 384, 2220, 2436, 2748, 2845
SstI GAGCTC 2 cut(s) 1668, 2763
StuI AGGCCT 1 cut(s) 1878
StyI CCWWGG 8 cut(s) 15, 190, 402, 894, 1413, 1433, 1472, 2695
TaaI ACNGT 8 cut(s) 880, 1027, 1364, 1399, 1484, 2179, 2628, 2842
TaiI ACGT 2 cut(s) 27, 2784
TasI AATT 5 cut(s) 444, 1137, 1650, 2145, 2675
TatI WGTACW 3 cut(s) 979, 1861, 2566
TauI GCSGC 2 cut(s) 371, 2258
TfiI GAWTC 4 cut(s) 205, 557, 1983, 2439
Tru1I TTAA 5 cut(s) 410, 474, 527, 1311, 2771
Tru9I TTAA 5 cut(s) 410, 474, 527, 1311, 2771
TscAI CASTG 8 cut(s) 556, 994, 1189, 1489, 1543, 1824, 2146, 2633
TseFI GTSAC 9 cut(s) 707, 889, 918, 989, 1154, 1304, 1541, 2317, 2491
Tsp45I GTSAC 9 cut(s) 707, 889, 918, 989, 1154, 1304, 1541, 2317, 2491
TspDTI ATGAA 8 cut(s) 187, 307, 1365, 1596, 1946, 1975, 2017, 2512
TspGWI ACGGA 4 cut(s) 293, 877, 1958, 2519
TspMI CCCGGG 1 cut(s) 455
TspRI CASTG 8 cut(s) 556, 994, 1189, 1489, 1543, 1824, 2146, 2633
Tth111I GACNNNGTC 2 cut(s) 293, 488
Van91I CCANNNNNTGG 2 cut(s) 1630, 2791
Vha464I CTTAAG 1 cut(s) 1310
XapI RAATTY 1 cut(s) 444
XbaI TCTAGA 1 cut(s) 2219
XceI RCATGY 1 cut(s) 1600
XcmI CCANNNNNNNNNTGG 3 cut(s) 662, 961, 1553
XmaI CCCGGG 1 cut(s) 455
XmaJI CCTAGG 1 cut(s) 190
XmiI GTMKAC 3 cut(s) 42, 1029, 2277
XspI CTAG 6 cut(s) 191, 384, 2220, 2436, 2748, 2845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.