RLG00000015753

ATPase 8, plasma

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011636
Physical Location & Seq
Forward (+)
1198131 .. 1202443
4313 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015753

Sequence Viewer

Length: 2808 bp
ATGGCCAATATCTCCTTAGAGGACGTGAAAAAGGAGAACGTCGACCTTGAGAAAATCCCGGTGGAGGAAGTGTTCGAGCAGCTGAAATGCTCGAAGGAGGGGTTGAGCTCAGAGGAGGGCACGAAGAGGATCCAGAAAGAGAGCAAGGTTCTCAAGTTCCTCGGGTTCATGTGGAATCCCTTATCATGGGTGATGGAGGCTGCTGCAATCATGGCCATTGTTTTGGCCAATGGAGGGGGCAAGCCTCCGGATTGGCCAGACTTCGTCGGTATTGTGGTGCTTCTGATCATCAACTCAACTATATCATTCATCGAAGAGAACAATGCCGGCAACGCTGCTGCGGCTCTGATGGCTAGTCTTGCCCCCAAGACCAAGGTGTTACGGGATGGAAAATGGGGTGAGCAAGAGGCAGAAATTTTGGTACCAGGGGATGTGATTAGCATCAAGTTGGGAGATATAGTCCCAGCTGATGCTCGTCTCCTGGAGGGCGATCCCTTAAAGATTGACCAAGCTGCTCTCACTGGTGAATCCCTACCGGTCACCAGGCACCCCGGGGATGAGGTCTTCTCTGGATCCACATGCAAGCAGGGCGAGATTGAGGCCGTTGTAATCGCAACTGGAGTCCACACTTTCTTTGGCAAGGCTGCACACTTGGTGGATAGCACCAACCAAGTTGGTCACTTCCAAAAGGTGTTGACATCCATTGGTAACTTCTGCATCTGCTCCATTGGAGTTGGAATGGTGATTGAGATACTAGTGATGTACCCCATTCAGCACAGGGCATATAGAGATGGTATTGACAATCTGTTGGTGCTCCTGATCGGAGGTATCCCCATTGCCATGCCAACGGTCTTATCCGTGACCATGGCTATTGGGTCTCACCGCTTGTCACAGCAAGGTGCCATCACCAAGAGGATGACAGCCATTGAAGAAATGGCAGGAATGGATGTCCTTTGCAGTGACAAGACCGGAACCCTCACCCTCAACAAGCTTACCGTCGACAAGAGTCTGATTGAGGTGTTCCCTAAGAACATGGACAAAGACACGGTGGTCTTGCTCTCTGCCAGGGCTTCCAGGGTCGAAAACCAGGACGCAATTGATGCCTCAATTGTTGGGATGCTGAGTGACCCCAAGGAGGCAAGAGCAGGGATCACTGAGGTACATTTCCTGCCCTTCAACCCTGTCGACAAGCGCACAGCAATCACCTACATCGATAGCAATGGCGACTGGCACAGATGCAGCAAGGGTGCTCCTGAGCAAATAATTGACCTATGTGCCCTTAAGGGAGAAGCGAGGAAGAAGGCCCATGAAGTCATTGACAACTATGCTAACCGAGGTCTTCGTTCATTGGGAGTTGCACGACAGACAGTATCAGAGAAGACCAAGGAAAGTGCTGGTGATGAATGGGAGTTTGTGGGTCTATTGCCTCTCTTTGACCCTCCGAGGCATGACAGTGCTGAGACTATCCGCCGTGCTCTTGAGCTTGGAGTCAATGTTAAGATGATCACTGGTGACCAACTGGCCATTGGAAAAGAGACTGGTCGAAGGCTTGGCATGGGCACCAACATGTATCCTTCATCTACCCTTCTTGGCGAAAGCAAGGACGAGTCCATTGCTTCGATTCCTGTTGATGAGCTCATTGAAAAGGCTGATGGATTTGCTGGAGTCTTCCCTGAGCACAAGTATGAGATTGTTAAGAGACTGCAGGAGAGGAAGCACATCTGTGGTATGACCGGAGATGGTGTGAATGATGCCCCGGCTCTCAAGAGAGCAGACATTGGCATCGCAGTGGATGATGCAACTGATGCAGCCAGGAGTGCCTCGGACATTGTACTGACTGAGCCAGGACTAAGTGTTATTGTGAGCGCCGTCTTGACAAGCAGAGCCATCTTCCAGAGGATGAAGAACTATACCATTTATGCTGTTTCCATCACAATCCGTATTGTGCTGGGATTCATGCTTCTTGCTCTCATATGGAAATATGACTTCCCACCATTCATGGTCTTGGTCATTGCAGTCTTGAACGACGGGACCATCATGACCATCTCTAAGGATAGAGTGAAGCCATCTCCTCTGCCTGACTCCTGGAAACTCAAGGAAATCTTCGCCACTGGAGTTGTCCTTGGTTCCTACATGGCTGTCATGACTGTGGTCTTCTTCTGGCTTGCACACAGCACTCTCTTTTTCTCTAAAATATTTGGTGTGAGACCTATTGAGGACCTTCAGCTTAACTCAGCTGTCTACCTCCAAGTGAGCATTCTCAGTCAAGCACTCATCTTTGTGACCCGATCAAGGAGCTGGTCCTTTGTTGAACGCCCTGGTACCATGCTTATGGTTGCCTTTCTTGCAGCGCAGTTGGTGGCTACCCTCATTGCCGTATATTGTGACTGGGGCTTTGCCAAAATCAATGGCATCGGATGGGGATGGGCAGGCGCTATCTGGGTCTACAGCATTGTCACCTATTTCCCTCTCGATATCTTCAAGTTCCTGATCCGTTATGCATTGAGTGGCCATGCCTGGGATAATGTTGTCCAGAACAAGACTGCCTTCACAACAAAGAATGACTATGGAAAGCCTGAGAGAGAGGCACAGTGGGCATCGGCTCAACGTACTCTCCATGGTCTCCAGGCTCCTCCGGAAATGTTCCATGGAAGCCACGGGGATGATCAATCCATGGCAGAGCAGGCCAAGAGGCGAGCAGAAGTTGCTAGGCTGAGGGAGCTCCACACCTTAAAGGGGCACGTTGAATCTGTGGTCAAGCTGAAGGGGCTAGACATCGACACCATCCAACAGCACTACACCGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

936

Amino Acids

102.54

Weight (kDa)

5.93

Isoelectric Point (pI)

33.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E1-E2_ATPase PF00122 120 - 219 2.5e-29 P-type ATPase actuator domain
Hydrolase PF00702 315 - 592 7.8e-14 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 378 - 483 1.1e-06 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011636)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07560 AT3G42640
fragaria_vesca FvH4_1g01450 FvH4_1g01450
malus_domestica MD02G1011600.v1.1
prunus_persica Prupe.7G258000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0086371
rosa_laevigata RLG00000015753
rosa_multiflora Rmu_co8318751.1_g000001 Rmu_sc0003572.1_g000001 Rmu_sc0005127.1_g000010
rosa_roxburghii Rroxscaffold_2G00154660
rosa_rugosa Rorug01G0466900 Rorug01G0467000
rosa_samantha Rh2AG017100 Rh2BG016900 Rh2CG017900 Rh2DG018600
rosa_wichuraiana Rw2G001450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1049
Acc65I GGTACC 2 cut(s) 421, 2321
AccB1I GGYRCC 5 cut(s) 421, 546, 899, 1559, 2321
AccI GTMKAC 5 cut(s) 42, 999, 1185, 2241, 2445
AccIII TCCGGA 2 cut(s) 247, 2635
AciI CCGC 3 cut(s) 341, 883, 1468
AclWI GGATC 7 cut(s) 124, 137, 485, 567, 580, 1157, 2485
AcoI YGGCCR 6 cut(s) 3, 213, 225, 254, 1521, 2509
AcsI RAATTY 1 cut(s) 414
AcuI CTGAAG 2 cut(s) 2207, 2783
AdeI CACNNNGTG 1 cut(s) 655
AfaI GTAC 6 cut(s) 423, 764, 1161, 1833, 2323, 2611
AfiI CCNNNNNNNGG 7 cut(s) 64, 186, 234, 1135, 2292, 2518, 2736
AflII CTTAAG 1 cut(s) 1280
AflIII ACRYGT 1 cut(s) 1566
AgeI ACCGGT 1 cut(s) 535
AgsI TTSAA 7 cut(s) 929, 1177, 1643, 2023, 2312, 2482, 2747
AhlI ACTAGT 1 cut(s) 754
AjiI CACGTC 1 cut(s) 25
AjuI GAANNNNNNNTTGG 2 cut(s) 2241, 2273
AleI CACNNNNGTG 1 cut(s) 1722
AloI GAACNNNNNNTCC 2 cut(s) 56, 88
Alw21I GWGCWC 7 cut(s) 110, 816, 1252, 1477, 1638, 1680, 2724
Alw26I GTCTC 7 cut(s) 482, 882, 1454, 1529, 1693, 2200, 2627
AlwI GGATC 7 cut(s) 124, 137, 485, 567, 580, 1157, 2485
Ama87I CYCGRG 2 cut(s) 161, 551
Aor13HI TCCGGA 2 cut(s) 247, 2635
AoxI GGCC 9 cut(s) 3, 213, 225, 254, 600, 1302, 1521, 2509, 2685
ApoI RAATTY 1 cut(s) 414
ArsI GACNNNNNNTTYG 2 cut(s) 2378, 2410
AsiGI ACCGGT 1 cut(s) 535
Asp718I GGTACC 2 cut(s) 421, 2321
AspLEI GCGC 4 cut(s) 1194, 1868, 2353, 2435
AspS9I GGNCC 4 cut(s) 1303, 2031, 2218, 2301
AsuC2I CCSGG 4 cut(s) 59, 552, 553, 1757
AvaI CYCGRG 2 cut(s) 161, 551
AvaII GGWCC 3 cut(s) 2031, 2218, 2301
BaeGI GKGCMC 4 cut(s) 122, 1279, 1562, 2742
BalI TGGCCA 6 cut(s) 5, 215, 227, 256, 1523, 2511
BamHI GGATCC 2 cut(s) 129, 572
BanI GGYRCC 5 cut(s) 421, 546, 899, 1559, 2321
BanII GRGCYC 3 cut(s) 110, 1638, 2724
BbsI GAAGAC 5 cut(s) 556, 1331, 1385, 1660, 2146
Bbv12I GWGCWC 7 cut(s) 110, 816, 1252, 1477, 1638, 1680, 2724
BbvCI CCTCAGC 1 cut(s) 2714
BceAI ACGGC 4 cut(s) 587, 1455, 1853, 2360
BcgI CGANNNNNNTGC 2 cut(s) 1595, 1629
BciVI GTATCC 2 cut(s) 839, 1581
BclI TGATCA 3 cut(s) 285, 1503, 2665
BcnI CCSGG 4 cut(s) 59, 552, 553, 1757
BcoDI GTCTC 7 cut(s) 482, 882, 1454, 1529, 1693, 2200, 2627
BcuI ACTAGT 1 cut(s) 754
BfaI CTAG 5 cut(s) 354, 755, 2709, 2771, 2806
BfmI CTRYAG 2 cut(s) 1703, 2446
BfoI RGCGCY 2 cut(s) 1869, 2436
BfrI CTTAAG 1 cut(s) 1280
BfuI GTATCC 2 cut(s) 839, 1581
Bme18I GGWCC 3 cut(s) 2031, 2218, 2301
BmeT110I CYCGRG 2 cut(s) 161, 551
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 4 cut(s) 1303, 2031, 2218, 2301
BmrI ACTGGG 1 cut(s) 2398
BmuI ACTGGG 1 cut(s) 2398
BoxI GACNNNNGTC 2 cut(s) 1005, 2150
BpiI GAAGAC 5 cut(s) 556, 1331, 1385, 1660, 2146
BplI GAGNNNNNCTC 2 cut(s) 551, 583
BpmI CTGGAG 5 cut(s) 503, 639, 1683, 2133, 2609
Bpu10I CCTNAGC 3 cut(s) 1254, 1674, 2714
BpuEI CTTGAG 5 cut(s) 68, 137, 1499, 1748, 2078
BpuMI CCSGG 4 cut(s) 59, 552, 553, 1757
Bsa29I ATCGAT 1 cut(s) 1212
BsaBI GATNNNNATC 1 cut(s) 440
BsaI GGTCTC 3 cut(s) 882, 2200, 2627
BsaWI WCCGGW 5 cut(s) 247, 535, 968, 1733, 2635
Bsc4I CCNNNNNNNGG 7 cut(s) 64, 186, 234, 1135, 2292, 2518, 2736
Bse118I RCCGGY 2 cut(s) 326, 535
Bse1I ACTGG 8 cut(s) 526, 622, 1232, 1513, 1524, 1543, 2116, 2393
Bse3DI GCAATG 5 cut(s) 834, 1225, 1611, 2010, 2370
Bse8I GATNNNNATC 1 cut(s) 440
BseAI TCCGGA 2 cut(s) 247, 2635
BseCI ATCGAT 1 cut(s) 1212
BseJI GATNNNNATC 1 cut(s) 440
BseLI CCNNNNNNNGG 7 cut(s) 64, 186, 234, 1135, 2292, 2518, 2736
BseMI GCAATG 5 cut(s) 834, 1225, 1611, 2010, 2370
BseNI ACTGG 8 cut(s) 526, 622, 1232, 1513, 1524, 1543, 2116, 2393
BseRI GAGGAG 3 cut(s) 128, 2061, 2622
BseSI GKGCMC 4 cut(s) 122, 1279, 1562, 2742
BseYI CCCAGC 2 cut(s) 463, 1948
BsgI GTGCAG 1 cut(s) 630
BshFI GGCC 9 cut(s) 5, 215, 227, 256, 602, 1304, 1523, 2511, 2687
BshNI GGYRCC 5 cut(s) 421, 546, 899, 1559, 2321
BshTI ACCGGT 1 cut(s) 535
BshVI ATCGAT 1 cut(s) 1212
BsiHKAI GWGCWC 7 cut(s) 110, 816, 1252, 1477, 1638, 1680, 2724
BsiHKCI CYCGRG 2 cut(s) 161, 551
BsiSI CCGG 9 cut(s) 59, 248, 327, 536, 552, 969, 1734, 1757, 2636
BslFI GGGAC 2 cut(s) 446, 2044
BslI CCNNNNNNNGG 7 cut(s) 64, 186, 234, 1135, 2292, 2518, 2736
BsmAI GTCTC 7 cut(s) 482, 882, 1454, 1529, 1693, 2200, 2627
BsmBI CGTCTC 1 cut(s) 482
BsmFI GGGAC 2 cut(s) 446, 2044
BsmI GAATGC 1 cut(s) 2256
BsnI GGCC 9 cut(s) 5, 215, 227, 256, 602, 1304, 1523, 2511, 2687
Bso31I GGTCTC 3 cut(s) 882, 2200, 2627
BsoBI CYCGRG 2 cut(s) 161, 551
Bsp13I TCCGGA 2 cut(s) 247, 2635
Bsp19I CCATGG 4 cut(s) 864, 2617, 2647, 2673
BspACI CCGC 3 cut(s) 341, 883, 1468
BspANI GGCC 9 cut(s) 5, 215, 227, 256, 602, 1304, 1523, 2511, 2687
BspDI ATCGAT 1 cut(s) 1212
BspEI TCCGGA 2 cut(s) 247, 2635
BspHI TCATGA 2 cut(s) 2037, 2142
BspMAI CTGCAG 1 cut(s) 1707
BspPI GGATC 7 cut(s) 124, 137, 485, 567, 580, 1157, 2485
BspT107I GGYRCC 5 cut(s) 421, 546, 899, 1559, 2321
BspTI CTTAAG 1 cut(s) 1280
BspTNI GGTCTC 3 cut(s) 882, 2200, 2627
BsrDI GCAATG 5 cut(s) 834, 1225, 1611, 2010, 2370
BsrFI RCCGGY 2 cut(s) 326, 535
BsrI ACTGG 8 cut(s) 526, 622, 1232, 1513, 1524, 1543, 2116, 2393
BssAI RCCGGY 2 cut(s) 326, 535
BssT1I CCWWGG 8 cut(s) 372, 864, 1131, 1383, 2122, 2617, 2647, 2673
Bst4CI ACNGT 8 cut(s) 850, 997, 1048, 1369, 1454, 2149, 2592, 2803
Bst6I CTCTTC 2 cut(s) 119, 309
BstAFI CTTAAG 1 cut(s) 1280
BstAPI GCANNNNNTGC 3 cut(s) 1100, 1805, 2705
BstC8I GCNNGC 7 cut(s) 242, 328, 584, 2166, 2431, 2685, 2697
BstDSI CCRYGG 5 cut(s) 864, 2617, 2647, 2656, 2673
BstEII GGTNACC 2 cut(s) 538, 1511
BstH2I RGCGCY 2 cut(s) 1869, 2436
BstHHI GCGC 4 cut(s) 1194, 1868, 2353, 2435
BstMAI GTCTC 7 cut(s) 482, 882, 1454, 1529, 1693, 2200, 2627
BstNSI RCATGY 2 cut(s) 582, 1570
BstPAI GACNNNNGTC 2 cut(s) 1005, 2150
BstPI GGTNACC 2 cut(s) 538, 1511
BstSFI CTRYAG 2 cut(s) 1703, 2446
BstSLI GKGCMC 4 cut(s) 122, 1279, 1562, 2742
BstV2I GAAGAC 5 cut(s) 556, 1331, 1385, 1660, 2146
BstX2I RGATCY 2 cut(s) 129, 572
BstXI CCANNNNNNTGG 2 cut(s) 223, 2332
BstYI RGATCY 2 cut(s) 129, 572
Bsu15I ATCGAT 1 cut(s) 1212
BsuI GTATCC 2 cut(s) 839, 1581
BsuRI GGCC 9 cut(s) 5, 215, 227, 256, 602, 1304, 1523, 2511, 2687
BsuTUI ATCGAT 1 cut(s) 1212
BtgI CCRYGG 5 cut(s) 864, 2617, 2647, 2656, 2673
BtgZI GCGATG 1 cut(s) 1768
BtrI CACGTC 1 cut(s) 25
BtsI GCAGTG 2 cut(s) 964, 1794
BtsIMutI CAGTG 8 cut(s) 519, 964, 1152, 1459, 1506, 1794, 2109, 2597
Cac8I GCNNGC 7 cut(s) 242, 328, 584, 2166, 2431, 2685, 2697
CciI TCATGA 2 cut(s) 2037, 2142
CfoI GCGC 4 cut(s) 1194, 1868, 2353, 2435
Cfr10I RCCGGY 2 cut(s) 326, 535
Cfr13I GGNCC 4 cut(s) 1303, 2031, 2218, 2301
Cfr9I CCCGGG 1 cut(s) 551
ClaI ATCGAT 1 cut(s) 1212
CseI GACGC 1 cut(s) 1100
Csp6I GTAC 6 cut(s) 422, 763, 1160, 1832, 2322, 2610
CspAI ACCGGT 1 cut(s) 535
CspCI CAANNNNNGTGG 2 cut(s) 2098, 2133
CviQI GTAC 6 cut(s) 422, 763, 1160, 1832, 2322, 2610
DraIII CACNNNGTG 1 cut(s) 655
DrdI GACNNNNNNGTC 1 cut(s) 1049
DseDI GACNNNNNNGTC 1 cut(s) 1049
EaeI YGGCCR 6 cut(s) 3, 213, 225, 254, 1521, 2509
Eam1104I CTCTTC 2 cut(s) 119, 309
EarI CTCTTC 2 cut(s) 119, 309
EciI GGCGGA 1 cut(s) 1457
Ecl136II GAGCTC 3 cut(s) 108, 1636, 2722
Eco130I CCWWGG 8 cut(s) 372, 864, 1131, 1383, 2122, 2617, 2647, 2673
Eco24I GRGCYC 3 cut(s) 110, 1638, 2724
Eco31I GGTCTC 3 cut(s) 882, 2200, 2627
Eco32I GATATC 1 cut(s) 2476
Eco47I GGWCC 3 cut(s) 2031, 2218, 2301
Eco53kI GAGCTC 3 cut(s) 108, 1636, 2722
Eco57I CTGAAG 2 cut(s) 2207, 2783
Eco88I CYCGRG 2 cut(s) 161, 551
Eco91I GGTNACC 2 cut(s) 538, 1511
EcoICRI GAGCTC 3 cut(s) 108, 1636, 2722
EcoO109I RGGNCCY 1 cut(s) 2218
EcoO65I GGTNACC 2 cut(s) 538, 1511
EcoRV GATATC 1 cut(s) 2476
EcoT14I CCWWGG 8 cut(s) 372, 864, 1131, 1383, 2122, 2617, 2647, 2673
EcoT22I ATGCAT 1 cut(s) 2503
EcoT38I GRGCYC 3 cut(s) 110, 1638, 2724
ErhI CCWWGG 8 cut(s) 372, 864, 1131, 1383, 2122, 2617, 2647, 2673
Esp3I CGTCTC 1 cut(s) 482
FalI AAGNNNNNCTT 4 cut(s) 2087, 2119, 2531, 2563
FaqI GGGAC 2 cut(s) 446, 2044
FauNDI CATATG 1 cut(s) 1973
FbaI TGATCA 3 cut(s) 285, 1503, 2665
FblI GTMKAC 5 cut(s) 42, 999, 1185, 2241, 2445
FriOI GRGCYC 3 cut(s) 110, 1638, 2724
FspBI CTAG 5 cut(s) 354, 755, 2709, 2771, 2806
GlaI GCGC 4 cut(s) 1193, 1867, 2352, 2434
GsaI CCCAGC 2 cut(s) 467, 1952
GsuI CTGGAG 5 cut(s) 503, 639, 1683, 2133, 2609
HaeII RGCGCY 2 cut(s) 1869, 2436
HaeIII GGCC 9 cut(s) 5, 215, 227, 256, 602, 1304, 1523, 2511, 2687
HapII CCGG 9 cut(s) 59, 248, 327, 536, 552, 969, 1734, 1757, 2636
HgaI GACGC 1 cut(s) 1100
HhaI GCGC 4 cut(s) 1194, 1868, 2353, 2435
Hin6I GCGC 4 cut(s) 1192, 1866, 2351, 2433
HinP1I GCGC 4 cut(s) 1192, 1866, 2351, 2433
HincII GTYRAC 4 cut(s) 43, 696, 1000, 1186
HindII GTYRAC 4 cut(s) 43, 696, 1000, 1186
HindIII AAGCTT 1 cut(s) 989
HpaII CCGG 9 cut(s) 59, 248, 327, 536, 552, 969, 1734, 1757, 2636
Hpy166II GTNNAC 7 cut(s) 43, 625, 696, 1000, 1186, 2242, 2446
Hpy188I TCNGA 9 cut(s) 112, 285, 348, 824, 1011, 1375, 1443, 1825, 2417
Hpy8I GTNNAC 7 cut(s) 43, 625, 696, 1000, 1186, 2242, 2446
Hpy99I CGWCG 4 cut(s) 44, 269, 1001, 2030
HpyAV CCTTC 9 cut(s) 88, 1183, 1294, 1539, 1584, 1595, 2231, 2557, 2758
HpyCH4III ACNGT 8 cut(s) 850, 997, 1048, 1369, 1454, 2149, 2592, 2803
HpyCH4IV ACGT 4 cut(s) 24, 39, 2608, 2742
HpySE526I ACGT 4 cut(s) 24, 39, 2608, 2742
HspAI GCGC 4 cut(s) 1192, 1866, 2351, 2433
Kpn2I TCCGGA 2 cut(s) 247, 2635
KpnI GGTACC 2 cut(s) 425, 2325
KroI GCCGGC 1 cut(s) 326
KroNI GCCGGC 1 cut(s) 328
Ksp22I TGATCA 3 cut(s) 285, 1503, 2665
LmnI GCTCC 7 cut(s) 728, 819, 1255, 2295, 2635, 2719, 2727
MaeI CTAG 5 cut(s) 354, 755, 2709, 2771, 2806
MaeII ACGT 4 cut(s) 24, 39, 2608, 2742
MfeI CAATTG 2 cut(s) 1095, 1107
MflI RGATCY 2 cut(s) 129, 572
MlsI TGGCCA 6 cut(s) 5, 215, 227, 256, 1523, 2511
MluCI AATT 4 cut(s) 414, 1095, 1107, 1263
MluNI TGGCCA 6 cut(s) 5, 215, 227, 256, 1523, 2511
MlyI GAGTC 6 cut(s) 630, 1015, 1497, 1616, 1674, 2075
MmeI TCCRAC 1 cut(s) 715
Mox20I TGGCCA 6 cut(s) 5, 215, 227, 256, 1523, 2511
Mph1103I ATGCAT 1 cut(s) 2503
MroI TCCGGA 2 cut(s) 247, 2635
MroNI GCCGGC 1 cut(s) 326
MscI TGGCCA 6 cut(s) 5, 215, 227, 256, 1523, 2511
MseI TTAA 6 cut(s) 497, 1281, 1497, 1695, 2229, 2732
Msp20I TGGCCA 6 cut(s) 5, 215, 227, 256, 1523, 2511
MspA1I CMGCKG 3 cut(s) 82, 467, 2237
MspCI CTTAAG 1 cut(s) 1280
MspI CCGG 9 cut(s) 59, 248, 327, 536, 552, 969, 1734, 1757, 2636
MunI CAATTG 2 cut(s) 1095, 1107
Mva1269I GAATGC 1 cut(s) 2256
NaeI GCCGGC 1 cut(s) 328
NciI CCSGG 4 cut(s) 59, 552, 553, 1757
NcoI CCATGG 4 cut(s) 864, 2617, 2647, 2673
NdeI CATATG 1 cut(s) 1973
NgoMIV GCCGGC 1 cut(s) 326
NsiI ATGCAT 1 cut(s) 2503
NspI RCATGY 2 cut(s) 582, 1570
OliI CACNNNNGTG 1 cut(s) 1722
PagI TCATGA 2 cut(s) 2037, 2142
PciI ACATGT 1 cut(s) 1566
PctI GAATGC 1 cut(s) 2256
PdiI GCCGGC 1 cut(s) 328
PfeI GAWTC 5 cut(s) 175, 527, 1621, 1953, 2747
PflFI GACNNNGTC 1 cut(s) 263
PfoI TCCNGGA 2 cut(s) 480, 2084
PinAI ACCGGT 1 cut(s) 535
PleI GAGTC 6 cut(s) 629, 1014, 1496, 1615, 1673, 2075
PpsI GAGTC 6 cut(s) 629, 1014, 1496, 1615, 1673, 2075
PpuMI RGGWCCY 1 cut(s) 2218
PscI ACATGT 1 cut(s) 1566
PshAI GACNNNNGTC 2 cut(s) 1005, 2150
Psp124BI GAGCTC 3 cut(s) 110, 1638, 2724
Psp5II RGGWCCY 1 cut(s) 2218
PspEI GGTNACC 2 cut(s) 538, 1511
PspFI CCCAGC 2 cut(s) 463, 1948
PspPI GGNCC 4 cut(s) 1303, 2031, 2218, 2301
PspPPI RGGWCCY 1 cut(s) 2218
PstI CTGCAG 1 cut(s) 1707
PsuI RGATCY 2 cut(s) 129, 572
PsyI GACNNNGTC 1 cut(s) 263
PvuII CAGCTG 3 cut(s) 82, 467, 2237
RsaI GTAC 6 cut(s) 423, 764, 1161, 1833, 2323, 2611
RsaNI GTAC 6 cut(s) 422, 763, 1160, 1832, 2322, 2610
SacI GAGCTC 3 cut(s) 110, 1638, 2724
SalI GTCGAC 3 cut(s) 41, 998, 1184
SaqAI TTAA 6 cut(s) 497, 1281, 1497, 1695, 2229, 2732
Sau96I GGNCC 4 cut(s) 1303, 2031, 2218, 2301
SchI GAGTC 6 cut(s) 630, 1015, 1497, 1616, 1674, 2075
SfcI CTRYAG 2 cut(s) 1703, 2446
SinI GGWCC 3 cut(s) 2031, 2218, 2301
SmaI CCCGGG 1 cut(s) 553
SmlI CTYRAG 6 cut(s) 47, 152, 1280, 1478, 1763, 2093
SmoI CTYRAG 6 cut(s) 47, 152, 1280, 1478, 1763, 2093
SpeI ACTAGT 1 cut(s) 754
Sse9I AATT 4 cut(s) 414, 1095, 1107, 1263
SsiI CCGC 3 cut(s) 341, 883, 1468
SspI AATATT 1 cut(s) 2196
SspMI CTAG 5 cut(s) 354, 755, 2709, 2771, 2806
SstI GAGCTC 3 cut(s) 110, 1638, 2724
StyI CCWWGG 8 cut(s) 372, 864, 1131, 1383, 2122, 2617, 2647, 2673
TaaI ACNGT 8 cut(s) 850, 997, 1048, 1369, 1454, 2149, 2592, 2803
TaiI ACGT 4 cut(s) 27, 42, 2611, 2745
TasI AATT 4 cut(s) 414, 1095, 1107, 1263
TatI WGTACW 1 cut(s) 1831
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 5 cut(s) 175, 527, 1621, 1953, 2747
Tru1I TTAA 6 cut(s) 497, 1281, 1497, 1695, 2229, 2732
Tru9I TTAA 6 cut(s) 497, 1281, 1497, 1695, 2229, 2732
TscAI CASTG 8 cut(s) 526, 964, 1159, 1459, 1513, 1794, 2116, 2597
TspDTI ATGAA 9 cut(s) 157, 298, 1323, 1335, 1416, 1566, 1916, 1945, 1987
TspGWI ACGGA 3 cut(s) 847, 1928, 2483
TspMI CCCGGG 1 cut(s) 551
TspRI CASTG 8 cut(s) 526, 964, 1159, 1459, 1513, 1794, 2116, 2597
Tth111I GACNNNGTC 1 cut(s) 263
Vha464I CTTAAG 1 cut(s) 1280
VpaK11BI GGWCC 3 cut(s) 2031, 2218, 2301
XapI RAATTY 1 cut(s) 414
XceI RCATGY 2 cut(s) 582, 1570
XcmI CCANNNNNNNNNTGG 3 cut(s) 632, 931, 1523
XmaI CCCGGG 1 cut(s) 551
XmiI GTMKAC 5 cut(s) 42, 999, 1185, 2241, 2445
XspI CTAG 5 cut(s) 354, 755, 2709, 2771, 2806
Zsp2I ATGCAT 1 cut(s) 2503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.