Rw2G001450

ATPase 8, plasma

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0011636
Physical Location & Seq
Forward (+)
1224345 .. 1229270
4926 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G001450.1

Sequence Viewer

Length: 2838 bp
ATGGCCAATATCTCCTTAGAGGACGTGAAAAAGGAGAACGTCGACCTTGAGAAAATCCCGGTGGAGGAAGTGTTCGAGCAGCTGAAATGCTCGAAGGAGGGGTTGAGCTCAGAGGAGGGTACGAAGAGGATCCAGGTTTTCGGCCCGAATAAGCTCGAGGAAAAGAAAGAGAGCAAGGTTCTCAAGTTCCTCGGGTTCATGTGGAATCCCTTATCATGGGTGATGGAGGCTGCTGCAATCATGGCCATTGTTTTGGCCAATGGAGGGGGAAAGCCTCCGGATTGGCCAGACTTCGTCGGTATTGTGGTGCTTCTGATCATCAACTCAACTATATCATTCATCGAAGAGAACAATGCCGGCAACGCTGCTGCGGCTCTGATGGCTAGTCTAGCCCCCAAGACCAAGGTGTTACGGGATGGAAAATGGGGTGAGCAAGAGGCAGAAATTTTGGTACCAGGGGATGTGATTAGCATCAAGTTGGGAGATATAGTCCCAGCTGATGCTCGTCTCCTGGAGGGCGATCCCTTAAAGATTGACCAAGCTGCTCTCACCGGTGAATCCCTACCGGTCACCAGGCACCCCGGGGATGAGGTCTTCTCTGGATCTACATGCAAGCAGGGCGAGATTGAGGCCGTTGTAATCGCAACTGGAGTCCACACTTTCTTTGGCAAGGCTGCACACTTGGTGGATAGCACCAACCAAGTTGGTCACTTCCAAAAGGTGTTGACATCCATTGGTAACTTCTGCATCTGCTCCATTGGAGTTGGAATGGCGATTGAGATACTGGTGATGTACCCCATTCAGCACAGGGCATATAGAGATGGTATTGACAATCTGTTGGTGCTCCTGATCGGAGGTATCCCCATTGCCATGCCAACGGTCTTATCCGTGACCATGGCTATTGGGTCTCACCGCTTGTCACAGCAAGGTGCCATCACCAAGAGGATGACAGCCATTGAAGAAATGGCAGGAATGGATGTCCTTTGCAGTGACAAGACCGGAACCCTCACCCTCAACAAGCTTACCGTCGACAAGAGTCTGATTGAGGTGTTCCCTAAGAACATGGACAAAGACACAGTGGTCTTGCTCTCTGCCAGGGCTTCCAGGGTTGAAAACCAGGATGCAATTGATGCCTCAATTGTTGGGATGCTAAGTGACCCCAAGGAGGCAAGAGAAGGGATCACTGAGGTACATTTCCTGCCCTTCAACCCCGTCGAGAAGCGCACAGCAATCACCTACATCGATAGCAATGGCGACTGGCACAGATGCAGCAAGGGTGCTCCTGAGCAAATAATTGACCTATGTGCCCTTAAGGGAGAAGCGAGGAAGAAGGCCCATGAAGTCATTGACAACTATGCTAACCGTGGTCTTCGTTCATTGGGAGTTGCACGACAGACAGTATCAGAGAAGACCAAGGAAAGTGCTGGTGATGAATGGGAGTTTGTGGGTCTATTGCCTCTCTTTGACCCTCCAAGGCATGACAGTGCTGAGACTATCCGCCGTGCTCTTGAGCTTGGTGTCAATGTTAAGATGATCACTGGTGACCAACTGGCCATTGGAAAAGAGACTGGTCGAAGGCTTGGCATGGGCACCAACATGTATCCTTCATCTACCCTTCTTGGCGAAAGCAAGGACGAGTCCATTGCTTCAATTCCTGTGGATGAGCTCATTGAAAAGGCTGATGGATTTGCTGGAGTCTTCCCTGAGCACAAGTATGAGATTGTTAAGAGACTGCAGGAGAGGAAGCACATCTGTGGTATGACCGGAGATGGTGTGAATGATGCCCCGGCTCTCAAGAGAGCAGACATTGGCATCGCAGTGGATGATGCAACTGATGCAGCCAGGAGTGCCTCGGACATTGTACTGACTGAGCCAGGACTAAGTGTTATTGTGAGCGCCGTCTTGACAAGCAGAGCCATCTTCCAGAGGATGAAGAATTATACCATTTATGCTGTTTCCATCACAATCCGTATTGTGGTGGGATTCATGCTTCTTGCTCTCATATGGAAATATGACTTCCCACCATTCATGGTCTTGGTCATTGCAGTCTTGAACGACGGGACCATCATGACCATCTCTAAGGATAGAGTGAAGCCATCTCCTCTGCCTGACTCCTGGAAACTCAAGGAAATCTTCGCCACTGGAGTTGTCCTTGGTTCCTACATGGCTGTCATGACTGTGGTCTTCTTCTGGCTTGCACACAGCACTCTCTTTTTCTCTAAAATATTTGGTGTGAGACCTATTGAGGACCTTCAGCTTAACTCAGCTGTCTACCTCCAAGTGAGCATTCTCAGTCAAGCACTCATCTTTGTGACCCGATCAAGGAGCTGGTCCTTTGTTGAACGCCCTGGTACCATGCTTATGGTTGCCTTTCTTGCAGCGCAGTTGGTGGCTACCCTCATTGCCGTATATTGTGACTGGGGCTTTGCCAAAATCAACGGTATCGGATGGGGATGGGCAGGCGCTATCTGGGTCTACAGCATTGTCACCTATTTCCCTCTCGATATCTTCAAGTTCCTGATCCGTTATGCATTGAGTGGCCATGCCTGGGATAATGTTGTCCAGAACAAGACTGCCTTCACAACAAAGAATGACTATGGAAAGCCTGAGAGAGAGGCACAGTGGGCATCGGCTCAGCGTACACTCCATGGTCTCCAGGCTCCTCCAGAAATGTTCCATGGAAGCCACGGGGATGATCAATCCATGGCTGAGCAGGCCAAGAGGCGAGCAGAAGTTGCTAGGCTGAGGGAGCTCCACACCTTAAAGGGGCACGTCGAATCTGTGGTCAAGCTGAAGGGGCTAGACATCGACACCATCCAACAACACTACACCGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

945

Amino Acids

103.71

Weight (kDa)

5.88

Isoelectric Point (pI)

33.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 18 - 81 8.2e-16 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 130 - 229 2.5e-29 P-type ATPase actuator domain
Hydrolase PF00702 325 - 602 6.5e-14 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 388 - 493 1.3e-06 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011636)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07560 AT3G42640
fragaria_vesca FvH4_1g01450 FvH4_1g01450
malus_domestica MD02G1011600.v1.1
prunus_persica Prupe.7G258000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0086371
rosa_laevigata RLG00000015753
rosa_multiflora Rmu_co8318751.1_g000001 Rmu_sc0003572.1_g000001 Rmu_sc0005127.1_g000010
rosa_roxburghii Rroxscaffold_2G00154660
rosa_rugosa Rorug01G0466900 Rorug01G0467000
rosa_samantha Rh2AG017100 Rh2BG016900 Rh2CG017900 Rh2DG018600
rosa_wichuraiana Rw2G001450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1079
Acc65I GGTACC 2 cut(s) 451, 2351
AccB1I GGYRCC 5 cut(s) 451, 576, 929, 1589, 2351
AccI GTMKAC 4 cut(s) 42, 1029, 2271, 2475
AccIII TCCGGA 1 cut(s) 277
AciI CCGC 3 cut(s) 371, 913, 1498
AclWI GGATC 6 cut(s) 124, 137, 515, 610, 1187, 2515
AcoI YGGCCR 6 cut(s) 3, 243, 255, 284, 1551, 2539
AcsI RAATTY 1 cut(s) 444
AcuI CTGAAG 2 cut(s) 2237, 2813
AdeI CACNNNGTG 1 cut(s) 685
AfaI GTAC 7 cut(s) 121, 453, 794, 1191, 1863, 2353, 2641
AfiI CCNNNNNNNGG 8 cut(s) 64, 216, 264, 1165, 1975, 2322, 2548, 2766
AflII CTTAAG 1 cut(s) 1310
AflIII ACRYGT 1 cut(s) 1596
AgeI ACCGGT 2 cut(s) 551, 565
AgsI TTSAA 8 cut(s) 959, 1112, 1207, 1650, 1673, 2053, 2342, 2512
AjiI CACGTC 2 cut(s) 25, 2773
AjuI GAANNNNNNNTTGG 2 cut(s) 2271, 2303
AleI CACNNNNGTG 1 cut(s) 1752
AloI GAACNNNNNNTCC 2 cut(s) 56, 88
Alw21I GWGCWC 7 cut(s) 110, 846, 1282, 1507, 1668, 1710, 2754
Alw26I GTCTC 7 cut(s) 512, 912, 1484, 1559, 1723, 2230, 2657
AlwI GGATC 6 cut(s) 124, 137, 515, 610, 1187, 2515
Ama87I CYCGRG 3 cut(s) 155, 191, 581
Aor13HI TCCGGA 1 cut(s) 277
ApoI RAATTY 1 cut(s) 444
ArsI GACNNNNNNTTYG 4 cut(s) 2408, 2440, 2769, 2801
AsiGI ACCGGT 2 cut(s) 551, 565
Asp718I GGTACC 2 cut(s) 451, 2351
AspLEI GCGC 4 cut(s) 1224, 1898, 2383, 2465
AspS9I GGNCC 5 cut(s) 143, 1333, 2061, 2248, 2331
AsuC2I CCSGG 4 cut(s) 59, 582, 583, 1787
AvaI CYCGRG 3 cut(s) 155, 191, 581
AvaII GGWCC 3 cut(s) 2061, 2248, 2331
BaeGI GKGCMC 3 cut(s) 1309, 1592, 2772
BalI TGGCCA 6 cut(s) 5, 245, 257, 286, 1553, 2541
BamHI GGATCC 1 cut(s) 129
BanI GGYRCC 5 cut(s) 451, 576, 929, 1589, 2351
BanII GRGCYC 3 cut(s) 110, 1668, 2754
BbsI GAAGAC 5 cut(s) 586, 1361, 1415, 1690, 2176
Bbv12I GWGCWC 7 cut(s) 110, 846, 1282, 1507, 1668, 1710, 2754
BbvCI CCTCAGC 1 cut(s) 2744
BceAI ACGGC 4 cut(s) 617, 1485, 1883, 2390
BcgI CGANNNNNNTGC 2 cut(s) 1625, 1659
BciVI GTATCC 2 cut(s) 869, 1611
BclI TGATCA 3 cut(s) 315, 1533, 2695
BcnI CCSGG 4 cut(s) 59, 582, 583, 1787
BcoDI GTCTC 7 cut(s) 512, 912, 1484, 1559, 1723, 2230, 2657
BfaI CTAG 5 cut(s) 384, 389, 2739, 2801, 2836
BfmI CTRYAG 2 cut(s) 1733, 2476
BfoI RGCGCY 2 cut(s) 1899, 2466
BfrI CTTAAG 1 cut(s) 1310
BfuI GTATCC 2 cut(s) 869, 1611
BlpI GCTNAGC 2 cut(s) 2634, 2709
Bme18I GGWCC 3 cut(s) 2061, 2248, 2331
BmeT110I CYCGRG 3 cut(s) 155, 191, 581
BmgBI CACGTC 2 cut(s) 25, 2773
BmgT120I GGNCC 5 cut(s) 143, 1333, 2061, 2248, 2331
BmrI ACTGGG 1 cut(s) 2428
BmuI ACTGGG 1 cut(s) 2428
BoxI GACNNNNGTC 2 cut(s) 1035, 2180
BpiI GAAGAC 5 cut(s) 586, 1361, 1415, 1690, 2176
BplI GAGNNNNNCTC 2 cut(s) 581, 613
BpmI CTGGAG 6 cut(s) 533, 669, 1713, 2163, 2639, 2649
Bpu10I CCTNAGC 3 cut(s) 1284, 1704, 2744
Bpu1102I GCTNAGC 2 cut(s) 2634, 2709
BpuEI CTTGAG 5 cut(s) 68, 167, 1529, 1778, 2108
BpuMI CCSGG 4 cut(s) 59, 582, 583, 1787
Bsa29I ATCGAT 1 cut(s) 1242
BsaBI GATNNNNATC 1 cut(s) 470
BsaI GGTCTC 3 cut(s) 912, 2230, 2657
BsaWI WCCGGW 5 cut(s) 277, 551, 565, 998, 1763
BsaXI ACNNNNNCTCC 6 cut(s) 56, 86, 474, 504, 1758, 1788
Bsc4I CCNNNNNNNGG 8 cut(s) 64, 216, 264, 1165, 1975, 2322, 2548, 2766
Bse118I RCCGGY 3 cut(s) 356, 551, 565
Bse1I ACTGG 8 cut(s) 652, 789, 1262, 1543, 1554, 1573, 2146, 2423
Bse3DI GCAATG 5 cut(s) 864, 1255, 1641, 2040, 2400
Bse8I GATNNNNATC 1 cut(s) 470
BseAI TCCGGA 1 cut(s) 277
BseCI ATCGAT 1 cut(s) 1242
BseJI GATNNNNATC 1 cut(s) 470
BseLI CCNNNNNNNGG 8 cut(s) 64, 216, 264, 1165, 1975, 2322, 2548, 2766
BseMI GCAATG 5 cut(s) 864, 1255, 1641, 2040, 2400
BseNI ACTGG 8 cut(s) 652, 789, 1262, 1543, 1554, 1573, 2146, 2423
BseRI GAGGAG 3 cut(s) 128, 2091, 2652
BseSI GKGCMC 3 cut(s) 1309, 1592, 2772
BseYI CCCAGC 1 cut(s) 493
BsgI GTGCAG 1 cut(s) 660
BshNI GGYRCC 5 cut(s) 451, 576, 929, 1589, 2351
BshTI ACCGGT 2 cut(s) 551, 565
BshVI ATCGAT 1 cut(s) 1242
BsiHKAI GWGCWC 7 cut(s) 110, 846, 1282, 1507, 1668, 1710, 2754
BsiHKCI CYCGRG 3 cut(s) 155, 191, 581
BsiSI CCGG 9 cut(s) 59, 278, 357, 552, 566, 582, 999, 1764, 1787
BslFI GGGAC 2 cut(s) 476, 2074
BslI CCNNNNNNNGG 8 cut(s) 64, 216, 264, 1165, 1975, 2322, 2548, 2766
BsmAI GTCTC 7 cut(s) 512, 912, 1484, 1559, 1723, 2230, 2657
BsmBI CGTCTC 1 cut(s) 512
BsmFI GGGAC 2 cut(s) 476, 2074
BsmI GAATGC 1 cut(s) 2286
Bso31I GGTCTC 3 cut(s) 912, 2230, 2657
BsoBI CYCGRG 3 cut(s) 155, 191, 581
Bsp13I TCCGGA 1 cut(s) 277
Bsp1720I GCTNAGC 2 cut(s) 2634, 2709
Bsp19I CCATGG 4 cut(s) 894, 2647, 2677, 2703
BspACI CCGC 3 cut(s) 371, 913, 1498
BspDI ATCGAT 1 cut(s) 1242
BspEI TCCGGA 1 cut(s) 277
BspHI TCATGA 2 cut(s) 2067, 2172
BspMAI CTGCAG 1 cut(s) 1737
BspPI GGATC 6 cut(s) 124, 137, 515, 610, 1187, 2515
BspT107I GGYRCC 5 cut(s) 451, 576, 929, 1589, 2351
BspTI CTTAAG 1 cut(s) 1310
BspTNI GGTCTC 3 cut(s) 912, 2230, 2657
BsrDI GCAATG 5 cut(s) 864, 1255, 1641, 2040, 2400
BsrFI RCCGGY 3 cut(s) 356, 551, 565
BsrI ACTGG 8 cut(s) 652, 789, 1262, 1543, 1554, 1573, 2146, 2423
BssAI RCCGGY 3 cut(s) 356, 551, 565
BssT1I CCWWGG 9 cut(s) 402, 894, 1161, 1413, 1472, 2152, 2647, 2677, 2703
Bst6I CTCTTC 2 cut(s) 119, 339
BstAFI CTTAAG 1 cut(s) 1310
BstAPI GCANNNNNTGC 3 cut(s) 1130, 1835, 2735
BstC8I GCNNGC 6 cut(s) 358, 614, 2196, 2461, 2715, 2727
BstDSI CCRYGG 6 cut(s) 894, 1363, 2647, 2677, 2686, 2703
BstEII GGTNACC 2 cut(s) 568, 1541
BstH2I RGCGCY 2 cut(s) 1899, 2466
BstHHI GCGC 4 cut(s) 1224, 1898, 2383, 2465
BstMAI GTCTC 7 cut(s) 512, 912, 1484, 1559, 1723, 2230, 2657
BstNSI RCATGY 2 cut(s) 612, 1600
BstPAI GACNNNNGTC 2 cut(s) 1035, 2180
BstPI GGTNACC 2 cut(s) 568, 1541
BstSFI CTRYAG 2 cut(s) 1733, 2476
BstSLI GKGCMC 3 cut(s) 1309, 1592, 2772
BstV2I GAAGAC 5 cut(s) 586, 1361, 1415, 1690, 2176
BstX2I RGATCY 2 cut(s) 129, 602
BstXI CCANNNNNNTGG 2 cut(s) 253, 2362
BstYI RGATCY 2 cut(s) 129, 602
Bsu15I ATCGAT 1 cut(s) 1242
BsuI GTATCC 2 cut(s) 869, 1611
BsuTUI ATCGAT 1 cut(s) 1242
BtgI CCRYGG 6 cut(s) 894, 1363, 2647, 2677, 2686, 2703
BtgZI GCGATG 1 cut(s) 1798
BtrI CACGTC 2 cut(s) 25, 2773
BtsI GCAGTG 2 cut(s) 994, 1824
BtsIMutI CAGTG 8 cut(s) 994, 1083, 1182, 1489, 1536, 1824, 2139, 2627
Cac8I GCNNGC 6 cut(s) 358, 614, 2196, 2461, 2715, 2727
CciI TCATGA 2 cut(s) 2067, 2172
CfoI GCGC 4 cut(s) 1224, 1898, 2383, 2465
Cfr10I RCCGGY 3 cut(s) 356, 551, 565
Cfr13I GGNCC 5 cut(s) 143, 1333, 2061, 2248, 2331
Cfr9I CCCGGG 1 cut(s) 581
ClaI ATCGAT 1 cut(s) 1242
Csp6I GTAC 7 cut(s) 120, 452, 793, 1190, 1862, 2352, 2640
CspAI ACCGGT 2 cut(s) 551, 565
CspCI CAANNNNNGTGG 4 cut(s) 1638, 1673, 2128, 2163
CviQI GTAC 7 cut(s) 120, 452, 793, 1190, 1862, 2352, 2640
DraIII CACNNNGTG 1 cut(s) 685
DrdI GACNNNNNNGTC 1 cut(s) 1079
DseDI GACNNNNNNGTC 1 cut(s) 1079
EaeI YGGCCR 6 cut(s) 3, 243, 255, 284, 1551, 2539
Eam1104I CTCTTC 2 cut(s) 119, 339
EarI CTCTTC 2 cut(s) 119, 339
EciI GGCGGA 1 cut(s) 1487
Ecl136II GAGCTC 3 cut(s) 108, 1666, 2752
Eco130I CCWWGG 9 cut(s) 402, 894, 1161, 1413, 1472, 2152, 2647, 2677, 2703
Eco24I GRGCYC 3 cut(s) 110, 1668, 2754
Eco31I GGTCTC 3 cut(s) 912, 2230, 2657
Eco32I GATATC 1 cut(s) 2506
Eco47I GGWCC 3 cut(s) 2061, 2248, 2331
Eco53kI GAGCTC 3 cut(s) 108, 1666, 2752
Eco57I CTGAAG 2 cut(s) 2237, 2813
Eco88I CYCGRG 3 cut(s) 155, 191, 581
Eco91I GGTNACC 2 cut(s) 568, 1541
EcoICRI GAGCTC 3 cut(s) 108, 1666, 2752
EcoO109I RGGNCCY 1 cut(s) 2248
EcoO65I GGTNACC 2 cut(s) 568, 1541
EcoRV GATATC 1 cut(s) 2506
EcoT14I CCWWGG 9 cut(s) 402, 894, 1161, 1413, 1472, 2152, 2647, 2677, 2703
EcoT22I ATGCAT 1 cut(s) 2533
EcoT38I GRGCYC 3 cut(s) 110, 1668, 2754
ErhI CCWWGG 9 cut(s) 402, 894, 1161, 1413, 1472, 2152, 2647, 2677, 2703
Esp3I CGTCTC 1 cut(s) 512
FalI AAGNNNNNCTT 4 cut(s) 2117, 2149, 2561, 2593
FaqI GGGAC 2 cut(s) 476, 2074
FauNDI CATATG 1 cut(s) 2003
FbaI TGATCA 3 cut(s) 315, 1533, 2695
FblI GTMKAC 4 cut(s) 42, 1029, 2271, 2475
FriOI GRGCYC 3 cut(s) 110, 1668, 2754
FspBI CTAG 5 cut(s) 384, 389, 2739, 2801, 2836
GlaI GCGC 4 cut(s) 1223, 1897, 2382, 2464
GsaI CCCAGC 1 cut(s) 497
GsuI CTGGAG 6 cut(s) 533, 669, 1713, 2163, 2639, 2649
HaeII RGCGCY 2 cut(s) 1899, 2466
HapII CCGG 9 cut(s) 59, 278, 357, 552, 566, 582, 999, 1764, 1787
HhaI GCGC 4 cut(s) 1224, 1898, 2383, 2465
Hin6I GCGC 4 cut(s) 1222, 1896, 2381, 2463
HinP1I GCGC 4 cut(s) 1222, 1896, 2381, 2463
HincII GTYRAC 3 cut(s) 43, 726, 1030
HindII GTYRAC 3 cut(s) 43, 726, 1030
HindIII AAGCTT 1 cut(s) 1019
HinfI GANTC 9 cut(s) 205, 557, 651, 1036, 1637, 1695, 1983, 2111, 2777
HpaII CCGG 9 cut(s) 59, 278, 357, 552, 566, 582, 999, 1764, 1787
Hpy166II GTNNAC 7 cut(s) 43, 655, 726, 1030, 2272, 2476, 2642
Hpy188I TCNGA 8 cut(s) 112, 315, 378, 854, 1041, 1405, 1855, 2447
Hpy8I GTNNAC 7 cut(s) 43, 655, 726, 1030, 2272, 2476, 2642
Hpy99I CGWCG 6 cut(s) 44, 299, 1031, 1217, 2060, 2777
HpyCH4IV ACGT 3 cut(s) 24, 39, 2772
HpySE526I ACGT 3 cut(s) 24, 39, 2772
HspAI GCGC 4 cut(s) 1222, 1896, 2381, 2463
Kpn2I TCCGGA 1 cut(s) 277
KpnI GGTACC 2 cut(s) 455, 2355
KroI GCCGGC 1 cut(s) 356
KroNI GCCGGC 1 cut(s) 358
Ksp22I TGATCA 3 cut(s) 315, 1533, 2695
LmnI GCTCC 7 cut(s) 758, 849, 1285, 2325, 2665, 2749, 2757
MaeI CTAG 5 cut(s) 384, 389, 2739, 2801, 2836
MaeII ACGT 3 cut(s) 24, 39, 2772
MfeI CAATTG 2 cut(s) 1125, 1137
MflI RGATCY 2 cut(s) 129, 602
MlsI TGGCCA 6 cut(s) 5, 245, 257, 286, 1553, 2541
MluCI AATT 6 cut(s) 444, 1125, 1137, 1293, 1650, 1936
MluNI TGGCCA 6 cut(s) 5, 245, 257, 286, 1553, 2541
MlyI GAGTC 5 cut(s) 660, 1045, 1646, 1704, 2105
MmeI TCCRAC 1 cut(s) 745
Mox20I TGGCCA 6 cut(s) 5, 245, 257, 286, 1553, 2541
Mph1103I ATGCAT 1 cut(s) 2533
MroI TCCGGA 1 cut(s) 277
MroNI GCCGGC 1 cut(s) 356
MscI TGGCCA 6 cut(s) 5, 245, 257, 286, 1553, 2541
MseI TTAA 6 cut(s) 527, 1311, 1527, 1725, 2259, 2762
Msp20I TGGCCA 6 cut(s) 5, 245, 257, 286, 1553, 2541
MspA1I CMGCKG 3 cut(s) 82, 497, 2267
MspCI CTTAAG 1 cut(s) 1310
MspI CCGG 9 cut(s) 59, 278, 357, 552, 566, 582, 999, 1764, 1787
MunI CAATTG 2 cut(s) 1125, 1137
Mva1269I GAATGC 1 cut(s) 2286
NaeI GCCGGC 1 cut(s) 358
NciI CCSGG 4 cut(s) 59, 582, 583, 1787
NcoI CCATGG 4 cut(s) 894, 2647, 2677, 2703
NdeI CATATG 1 cut(s) 2003
NgoMIV GCCGGC 1 cut(s) 356
NsiI ATGCAT 1 cut(s) 2533
NspI RCATGY 2 cut(s) 612, 1600
OliI CACNNNNGTG 1 cut(s) 1752
PaeR7I CTCGAG 1 cut(s) 155
PagI TCATGA 2 cut(s) 2067, 2172
PciI ACATGT 1 cut(s) 1596
PctI GAATGC 1 cut(s) 2286
PdiI GCCGGC 1 cut(s) 358
PfeI GAWTC 4 cut(s) 205, 557, 1983, 2777
PflFI GACNNNGTC 1 cut(s) 293
PfoI TCCNGGA 2 cut(s) 510, 2114
PinAI ACCGGT 2 cut(s) 551, 565
PleI GAGTC 5 cut(s) 659, 1044, 1645, 1703, 2105
PpsI GAGTC 5 cut(s) 659, 1044, 1645, 1703, 2105
PpuMI RGGWCCY 1 cut(s) 2248
PscI ACATGT 1 cut(s) 1596
PshAI GACNNNNGTC 2 cut(s) 1035, 2180
Psp124BI GAGCTC 3 cut(s) 110, 1668, 2754
Psp5II RGGWCCY 1 cut(s) 2248
PspEI GGTNACC 2 cut(s) 568, 1541
PspFI CCCAGC 1 cut(s) 493
PspPI GGNCC 5 cut(s) 143, 1333, 2061, 2248, 2331
PspPPI RGGWCCY 1 cut(s) 2248
PspXI VCTCGAGB 1 cut(s) 155
PstI CTGCAG 1 cut(s) 1737
PsuI RGATCY 2 cut(s) 129, 602
PsyI GACNNNGTC 1 cut(s) 293
PvuII CAGCTG 3 cut(s) 82, 497, 2267
RsaI GTAC 7 cut(s) 121, 453, 794, 1191, 1863, 2353, 2641
RsaNI GTAC 7 cut(s) 120, 452, 793, 1190, 1862, 2352, 2640
SacI GAGCTC 3 cut(s) 110, 1668, 2754
SalI GTCGAC 2 cut(s) 41, 1028
SaqAI TTAA 6 cut(s) 527, 1311, 1527, 1725, 2259, 2762
Sau96I GGNCC 5 cut(s) 143, 1333, 2061, 2248, 2331
SchI GAGTC 5 cut(s) 660, 1045, 1646, 1704, 2105
SfcI CTRYAG 2 cut(s) 1733, 2476
Sfr274I CTCGAG 1 cut(s) 155
SgrAI CRCCGGYG 1 cut(s) 551
SinI GGWCC 3 cut(s) 2061, 2248, 2331
SlaI CTCGAG 1 cut(s) 155
SmaI CCCGGG 1 cut(s) 583
SmlI CTYRAG 7 cut(s) 47, 155, 182, 1310, 1508, 1793, 2123
SmoI CTYRAG 7 cut(s) 47, 155, 182, 1310, 1508, 1793, 2123
Sse9I AATT 6 cut(s) 444, 1125, 1137, 1293, 1650, 1936
SsiI CCGC 3 cut(s) 371, 913, 1498
SspI AATATT 1 cut(s) 2226
SspMI CTAG 5 cut(s) 384, 389, 2739, 2801, 2836
SstI GAGCTC 3 cut(s) 110, 1668, 2754
StyI CCWWGG 9 cut(s) 402, 894, 1161, 1413, 1472, 2152, 2647, 2677, 2703
TaiI ACGT 3 cut(s) 27, 42, 2775
TasI AATT 6 cut(s) 444, 1125, 1137, 1293, 1650, 1936
TatI WGTACW 1 cut(s) 1861
TauI GCSGC 1 cut(s) 374
TfiI GAWTC 4 cut(s) 205, 557, 1983, 2777
Tru1I TTAA 6 cut(s) 527, 1311, 1527, 1725, 2259, 2762
Tru9I TTAA 6 cut(s) 527, 1311, 1527, 1725, 2259, 2762
TscAI CASTG 8 cut(s) 994, 1083, 1189, 1489, 1543, 1824, 2146, 2627
TspDTI ATGAA 9 cut(s) 187, 328, 1353, 1365, 1446, 1596, 1946, 1975, 2017
TspGWI ACGGA 3 cut(s) 877, 1958, 2513
TspMI CCCGGG 1 cut(s) 581
TspRI CASTG 8 cut(s) 994, 1083, 1189, 1489, 1543, 1824, 2146, 2627
Tth111I GACNNNGTC 1 cut(s) 293
Vha464I CTTAAG 1 cut(s) 1310
VpaK11BI GGWCC 3 cut(s) 2061, 2248, 2331
XapI RAATTY 1 cut(s) 444
XceI RCATGY 2 cut(s) 612, 1600
XcmI CCANNNNNNNNNTGG 3 cut(s) 662, 961, 1553
XhoI CTCGAG 1 cut(s) 155
XmaI CCCGGG 1 cut(s) 581
XmiI GTMKAC 4 cut(s) 42, 1029, 2271, 2475
XspI CTAG 5 cut(s) 384, 389, 2739, 2801, 2836
Zsp2I ATGCAT 1 cut(s) 2533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.