Rh2AG017100

ATPase 8, plasma

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0011636
Physical Location & Seq
Forward (+)
1321239 .. 1326574
5336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG017100.1

Sequence Viewer

Length: 2487 bp
ATGGCCAATATCTCCTTAGAGGACGTGAAAAAGGAGAACGTCGACCTTGAGAAAATCCCGGTGGAGGAAGTGTTCGAGCAGCTGAAATGCTCGAAGGAGGGGTTGAGCTCAGAGGAGGGCACGAAGAGGATCCAGGTTTTCGGCCCGAATAAGCTCGAGGAAAAGAAAGAGAGCAAGGTTCTCAAGTTCCTCGGGTTCATGTGGAATCCCTTATCATGGGTGATGGAGGCTGCTGCAATCATGGCCATTGTTTTGGCCAATGGAGGGGGAAAGCCTCCGGATTGGCCAGACTTCGTCGGTATTGTGGTGCTTCTGATCATCAACTCAACTATATCATTCATCGAAGAGAACAATGCCGGCAACGCTGCTGCGGCTCTGATGGCTAGTCTAGCCCCCAAGACCAAGGTGTTACGGGATGGAAAATGGGGTGAGCAAGAGGCAGAAATTTTGGTACCAGGGGATGTGATTAGCATCAAGTTGGGAGATATAGTCCCAGCTGATGCTCGTCTCCTGGAGGGCGATCCCTTAAAGATTGACCAAGCTGCTCTCACCGGTGAATCCCTACCGGTCACCAGGCACCCCGGGGATGAGGTCTTCTCTGGATCTACATGCAAGCAGGGCGAGATTGAGGCCGTTGTAATCGCAACTGGAGTCCACACTTTCTTTGGCAAGGCTGCACACTTGGTGGATAGCACCAACCAAGTTGGTCACTTCCAAAAGGTGTTGACATCCATTGGTAACTTCTGCATCTGCTCCATTGGAGTTGGAATGGCGATTGAGATACTGGTGATGTACCCCATTCAGCACAGGGCATATAGAGATGGTATTGACAATCTGTTGGTGCTCTTGATCGGTGGTATCCCCATTGCCATGCCAACGGTCTTATCCGTGACCATGGCTATTGGGTCTCACCGCTTGTCACAGCAAGGTGCCATCACCAAGAGGATGACAGCCATTGAAGAAATGGCAGGAATGGATGTCCTTTGCAGTGACAAGACCGGAACCCTCACCCTCAACAAGCTTACCGTCGACAAGAGTCTGATTGAGGTGTTCCCTAAGAACATGGACAAAGACACAGTGGTCTTGCTCTCTGCCAGGGCTTCCAGGGTTGAAAACCAGGATGCAATTGATGCCTCAATTGTTGGGATGCTAAGTGACCCCAAGGAGGCAAGAGAAGGGATCACTGAGGTACATTTCCTGCCCTTCAACCCCGTCGAGAAGCGCACAGCAATCACCTACATCGATAGCAATGGCGACTGGCACAGATGCAGCAAGGGTGCTCCTGAGCAAATAATTGACCTATGTGCCCTTAAGGGAGAAGCGAGGAAGAAGGCCCATGAAGTCATTGACAACTATGCTAACCGTGGTCTTCGTTCATTGGGAGTTGCACGACAGACAGTATCAGAGAAGACCAAGGAAAGTGCTGGTGATGAATGGGAGTTTGTGGGTCTATTGCCTCTCTTTGACCCTCCAAGGCATGACAGTGCTGAGACTATCCGCCGTGCTCTTGAGCTTGGTGTCAATGTTAAGATGATCACTGGTGACCAACTGGCCATTGGAAAAGAGACTGGTCGAAGGCTTGGCATGGGCACCAACATGTATCCTTCATCTACCCTTCTTGGCGAAAGCAAGGACGAGTCCATTGCTTCAATTCCTGTGGATGAGCTCATTGAAAAGGCTGATGGATTTGCTGGAGTCTTCCCTGAGCACAAGTATGAGATTGTTAAGAGACTGCAGGAGAGGAAGCACATCTGTGGTATGACCGGAGATGGTGTGAATGATGCCCCGGCTCTCAAGAGAGCAGACATTGGCATCGCAGTGGATGATGCAACTGATGCAGCCAGGAGTGCCTCGGACATTGTACTGACTGAGCCAGGACTAAGTGTTATTGTGAGCGCCGTCTTGACAAGCAGAGCCATCTTCCAGAGGATGAAGAATTATACCATTTATGCTGTTTCCATCACAATCCGTATTGTGGTGGGATTCATGCTTCTTGCTCTCATATGGAAATATGACTTCCCACCATTCATGGTCTTGGTCATTGCAGTCTTGAACGACGGGACCATCATGACCATCTCTAAGGATAGAGTGAAGCCATCTCCTCTGCCTGACTCCTGGAAACTCAAGGAAATCTTCGCCACTGGAGTTGTCCTTGGTTCCTACATGGCTGTCATGACTGTGGTCTTCTTCTGGCTTGCACACAGCACTCTCTTTTTCTCTAAAATATTTGGTGTGAGACCTATTGAGGACCTTCAGCTTAACTCAGCTGTCTACCTCCAAGTGAGCATTCTCAGTCAAGCACTCATCTTTGTGACCCGATCAAGGAGCTGGTCCTTTGTTGAACGCCCTGGTACCATGCTTATGGTTGCCTTTCTTGCAGCGCAGTTGGTTAGTGGCTTATTTATGTCCATTGATCAAACTAGCTTCATAAATTTTGTCATCTTTAATAGTTTGCTGATCACCATCTACACTTCATACCTTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

828

Amino Acids

90.48

Weight (kDa)

5.48

Isoelectric Point (pI)

34.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 18 - 81 6.9e-16 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 130 - 229 2.1e-29 P-type ATPase actuator domain
Hydrolase PF00702 325 - 602 5.1e-14 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 388 - 493 1.1e-06 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011636)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07560 AT3G42640
fragaria_vesca FvH4_1g01450 FvH4_1g01450
malus_domestica MD02G1011600.v1.1
prunus_persica Prupe.7G258000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0086371
rosa_laevigata RLG00000015753
rosa_multiflora Rmu_co8318751.1_g000001 Rmu_sc0003572.1_g000001 Rmu_sc0005127.1_g000010
rosa_roxburghii Rroxscaffold_2G00154660
rosa_rugosa Rorug01G0466900 Rorug01G0467000
rosa_samantha Rh2AG017100 Rh2BG016900 Rh2CG017900 Rh2DG018600
rosa_wichuraiana Rw2G001450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1079
Acc65I GGTACC 2 cut(s) 451, 2351
AccB1I GGYRCC 5 cut(s) 451, 576, 929, 1589, 2351
AccI GTMKAC 3 cut(s) 42, 1029, 2271
AccIII TCCGGA 1 cut(s) 277
AciI CCGC 3 cut(s) 371, 913, 1498
AclWI GGATC 5 cut(s) 124, 137, 515, 610, 1187
AcoI YGGCCR 5 cut(s) 3, 243, 255, 284, 1551
AcsI RAATTY 2 cut(s) 444, 2431
AcuI CTGAAG 1 cut(s) 2237
AdeI CACNNNGTG 1 cut(s) 685
AfaI GTAC 5 cut(s) 453, 794, 1191, 1863, 2353
AfiI CCNNNNNNNGG 6 cut(s) 64, 216, 264, 1165, 1975, 2322
AflII CTTAAG 1 cut(s) 1310
AflIII ACRYGT 1 cut(s) 1596
AgeI ACCGGT 2 cut(s) 551, 565
AgsI TTSAA 7 cut(s) 959, 1112, 1207, 1650, 1673, 2053, 2342
AjiI CACGTC 1 cut(s) 25
AjuI GAANNNNNNNTTGG 2 cut(s) 2271, 2303
AleI CACNNNNGTG 1 cut(s) 1752
AloI GAACNNNNNNTCC 2 cut(s) 56, 88
Alw21I GWGCWC 6 cut(s) 110, 846, 1282, 1507, 1668, 1710
Alw26I GTCTC 6 cut(s) 512, 912, 1484, 1559, 1723, 2230
AlwI GGATC 5 cut(s) 124, 137, 515, 610, 1187
Ama87I CYCGRG 3 cut(s) 155, 191, 581
Aor13HI TCCGGA 1 cut(s) 277
AoxI GGCC 8 cut(s) 3, 142, 243, 255, 284, 630, 1332, 1551
ApoI RAATTY 2 cut(s) 444, 2431
AsiGI ACCGGT 2 cut(s) 551, 565
Asp718I GGTACC 2 cut(s) 451, 2351
AspLEI GCGC 3 cut(s) 1224, 1898, 2383
AspS9I GGNCC 5 cut(s) 143, 1333, 2061, 2248, 2331
AsuC2I CCSGG 4 cut(s) 59, 582, 583, 1787
AvaI CYCGRG 3 cut(s) 155, 191, 581
AvaII GGWCC 3 cut(s) 2061, 2248, 2331
BaeGI GKGCMC 3 cut(s) 122, 1309, 1592
BalI TGGCCA 5 cut(s) 5, 245, 257, 286, 1553
BamHI GGATCC 1 cut(s) 129
BanI GGYRCC 5 cut(s) 451, 576, 929, 1589, 2351
BanII GRGCYC 2 cut(s) 110, 1668
BbsI GAAGAC 5 cut(s) 586, 1361, 1415, 1690, 2176
Bbv12I GWGCWC 6 cut(s) 110, 846, 1282, 1507, 1668, 1710
BceAI ACGGC 3 cut(s) 617, 1485, 1883
BcgI CGANNNNNNTGC 2 cut(s) 1625, 1659
BciVI GTATCC 2 cut(s) 869, 1611
BclI TGATCA 4 cut(s) 315, 1533, 2413, 2457
BcnI CCSGG 4 cut(s) 59, 582, 583, 1787
BcoDI GTCTC 6 cut(s) 512, 912, 1484, 1559, 1723, 2230
BfaI CTAG 3 cut(s) 384, 389, 2421
BfmI CTRYAG 1 cut(s) 1733
BfoI RGCGCY 1 cut(s) 1899
BfrI CTTAAG 1 cut(s) 1310
BfuI GTATCC 2 cut(s) 869, 1611
Bme18I GGWCC 3 cut(s) 2061, 2248, 2331
BmeT110I CYCGRG 3 cut(s) 155, 191, 581
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 5 cut(s) 143, 1333, 2061, 2248, 2331
BmiI GGNNCC 9 cut(s) 131, 453, 578, 931, 1003, 1591, 2062, 2158, 2353
BoxI GACNNNNGTC 2 cut(s) 1035, 2180
BpiI GAAGAC 5 cut(s) 586, 1361, 1415, 1690, 2176
BplI GAGNNNNNCTC 2 cut(s) 581, 613
BpmI CTGGAG 4 cut(s) 533, 669, 1713, 2163
Bpu10I CCTNAGC 2 cut(s) 1284, 1704
BpuEI CTTGAG 5 cut(s) 68, 167, 1529, 1778, 2108
BpuMI CCSGG 4 cut(s) 59, 582, 583, 1787
Bsa29I ATCGAT 1 cut(s) 1242
BsaBI GATNNNNATC 2 cut(s) 470, 2462
BsaI GGTCTC 2 cut(s) 912, 2230
BsaWI WCCGGW 5 cut(s) 277, 551, 565, 998, 1763
BsaXI ACNNNNNCTCC 6 cut(s) 56, 86, 474, 504, 1758, 1788
Bsc4I CCNNNNNNNGG 6 cut(s) 64, 216, 264, 1165, 1975, 2322
Bse118I RCCGGY 3 cut(s) 356, 551, 565
Bse1I ACTGG 7 cut(s) 652, 789, 1262, 1543, 1554, 1573, 2146
Bse3DI GCAATG 4 cut(s) 864, 1255, 1641, 2040
Bse8I GATNNNNATC 2 cut(s) 470, 2462
BseAI TCCGGA 1 cut(s) 277
BseCI ATCGAT 1 cut(s) 1242
BseJI GATNNNNATC 2 cut(s) 470, 2462
BseLI CCNNNNNNNGG 6 cut(s) 64, 216, 264, 1165, 1975, 2322
BseMI GCAATG 4 cut(s) 864, 1255, 1641, 2040
BseMII CTCAG 8 cut(s) 123, 1176, 1275, 1479, 1695, 1860, 2277, 2305
BseNI ACTGG 7 cut(s) 652, 789, 1262, 1543, 1554, 1573, 2146
BseRI GAGGAG 2 cut(s) 128, 2091
BseSI GKGCMC 3 cut(s) 122, 1309, 1592
BseYI CCCAGC 1 cut(s) 493
BsgI GTGCAG 1 cut(s) 660
BshFI GGCC 8 cut(s) 5, 144, 245, 257, 286, 632, 1334, 1553
BshNI GGYRCC 5 cut(s) 451, 576, 929, 1589, 2351
BshTI ACCGGT 2 cut(s) 551, 565
BshVI ATCGAT 1 cut(s) 1242
BsiHKAI GWGCWC 6 cut(s) 110, 846, 1282, 1507, 1668, 1710
BsiHKCI CYCGRG 3 cut(s) 155, 191, 581
BsiSI CCGG 9 cut(s) 59, 278, 357, 552, 566, 582, 999, 1764, 1787
BslFI GGGAC 2 cut(s) 476, 2074
BslI CCNNNNNNNGG 6 cut(s) 64, 216, 264, 1165, 1975, 2322
BsmAI GTCTC 6 cut(s) 512, 912, 1484, 1559, 1723, 2230
BsmBI CGTCTC 1 cut(s) 512
BsmFI GGGAC 2 cut(s) 476, 2074
BsmI GAATGC 1 cut(s) 2286
BsnI GGCC 8 cut(s) 5, 144, 245, 257, 286, 632, 1334, 1553
Bso31I GGTCTC 2 cut(s) 912, 2230
BsoBI CYCGRG 3 cut(s) 155, 191, 581
Bsp1286I GDGCHC 9 cut(s) 110, 122, 846, 1282, 1309, 1507, 1592, 1668, 1710
Bsp13I TCCGGA 1 cut(s) 277
Bsp19I CCATGG 1 cut(s) 894
BspACI CCGC 3 cut(s) 371, 913, 1498
BspANI GGCC 8 cut(s) 5, 144, 245, 257, 286, 632, 1334, 1553
BspCNI CTCAG 8 cut(s) 122, 1177, 1276, 1480, 1696, 1861, 2276, 2304
BspDI ATCGAT 1 cut(s) 1242
BspEI TCCGGA 1 cut(s) 277
BspHI TCATGA 2 cut(s) 2067, 2172
BspLI GGNNCC 9 cut(s) 131, 453, 578, 931, 1003, 1591, 2062, 2158, 2353
BspMAI CTGCAG 1 cut(s) 1737
BspPI GGATC 5 cut(s) 124, 137, 515, 610, 1187
BspT107I GGYRCC 5 cut(s) 451, 576, 929, 1589, 2351
BspTI CTTAAG 1 cut(s) 1310
BspTNI GGTCTC 2 cut(s) 912, 2230
BsrDI GCAATG 4 cut(s) 864, 1255, 1641, 2040
BsrFI RCCGGY 3 cut(s) 356, 551, 565
BsrI ACTGG 7 cut(s) 652, 789, 1262, 1543, 1554, 1573, 2146
BssAI RCCGGY 3 cut(s) 356, 551, 565
BssT1I CCWWGG 6 cut(s) 402, 894, 1161, 1413, 1472, 2152
Bst4CI ACNGT 7 cut(s) 880, 1027, 1078, 1364, 1399, 1484, 2179
Bst6I CTCTTC 2 cut(s) 119, 339
BstAFI CTTAAG 1 cut(s) 1310
BstAPI GCANNNNNTGC 2 cut(s) 1130, 1835
BstC8I GCNNGC 3 cut(s) 358, 614, 2196
BstDSI CCRYGG 2 cut(s) 894, 1363
BstEII GGTNACC 2 cut(s) 568, 1541
BstH2I RGCGCY 1 cut(s) 1899
BstHHI GCGC 3 cut(s) 1224, 1898, 2383
BstMAI GTCTC 6 cut(s) 512, 912, 1484, 1559, 1723, 2230
BstNSI RCATGY 2 cut(s) 612, 1600
BstPAI GACNNNNGTC 2 cut(s) 1035, 2180
BstPI GGTNACC 2 cut(s) 568, 1541
BstSFI CTRYAG 1 cut(s) 1733
BstSLI GKGCMC 3 cut(s) 122, 1309, 1592
BstV2I GAAGAC 5 cut(s) 586, 1361, 1415, 1690, 2176
BstX2I RGATCY 2 cut(s) 129, 602
BstXI CCANNNNNNTGG 2 cut(s) 253, 2362
BstYI RGATCY 2 cut(s) 129, 602
Bsu15I ATCGAT 1 cut(s) 1242
BsuI GTATCC 2 cut(s) 869, 1611
BsuRI GGCC 8 cut(s) 5, 144, 245, 257, 286, 632, 1334, 1553
BsuTUI ATCGAT 1 cut(s) 1242
BtgI CCRYGG 2 cut(s) 894, 1363
BtgZI GCGATG 1 cut(s) 1798
BtrI CACGTC 1 cut(s) 25
BtsI GCAGTG 2 cut(s) 994, 1824
BtsIMutI CAGTG 7 cut(s) 994, 1083, 1182, 1489, 1536, 1824, 2139
Cac8I GCNNGC 3 cut(s) 358, 614, 2196
CciI TCATGA 2 cut(s) 2067, 2172
CfoI GCGC 3 cut(s) 1224, 1898, 2383
Cfr10I RCCGGY 3 cut(s) 356, 551, 565
Cfr13I GGNCC 5 cut(s) 143, 1333, 2061, 2248, 2331
Cfr9I CCCGGG 1 cut(s) 581
ClaI ATCGAT 1 cut(s) 1242
Csp6I GTAC 5 cut(s) 452, 793, 1190, 1862, 2352
CspAI ACCGGT 2 cut(s) 551, 565
CspCI CAANNNNNGTGG 4 cut(s) 1638, 1673, 2128, 2163
CviQI GTAC 5 cut(s) 452, 793, 1190, 1862, 2352
DraIII CACNNNGTG 1 cut(s) 685
DrdI GACNNNNNNGTC 1 cut(s) 1079
DseDI GACNNNNNNGTC 1 cut(s) 1079
EaeI YGGCCR 5 cut(s) 3, 243, 255, 284, 1551
Eam1104I CTCTTC 2 cut(s) 119, 339
EarI CTCTTC 2 cut(s) 119, 339
EciI GGCGGA 1 cut(s) 1487
Ecl136II GAGCTC 2 cut(s) 108, 1666
Eco130I CCWWGG 6 cut(s) 402, 894, 1161, 1413, 1472, 2152
Eco24I GRGCYC 2 cut(s) 110, 1668
Eco31I GGTCTC 2 cut(s) 912, 2230
Eco47I GGWCC 3 cut(s) 2061, 2248, 2331
Eco53kI GAGCTC 2 cut(s) 108, 1666
Eco57I CTGAAG 1 cut(s) 2237
Eco88I CYCGRG 3 cut(s) 155, 191, 581
Eco91I GGTNACC 2 cut(s) 568, 1541
EcoICRI GAGCTC 2 cut(s) 108, 1666
EcoO109I RGGNCCY 1 cut(s) 2248
EcoO65I GGTNACC 2 cut(s) 568, 1541
EcoT14I CCWWGG 6 cut(s) 402, 894, 1161, 1413, 1472, 2152
EcoT38I GRGCYC 2 cut(s) 110, 1668
ErhI CCWWGG 6 cut(s) 402, 894, 1161, 1413, 1472, 2152
Esp3I CGTCTC 1 cut(s) 512
FalI AAGNNNNNCTT 2 cut(s) 2117, 2149
FaqI GGGAC 2 cut(s) 476, 2074
FauNDI CATATG 1 cut(s) 2003
FbaI TGATCA 4 cut(s) 315, 1533, 2413, 2457
FblI GTMKAC 3 cut(s) 42, 1029, 2271
FriOI GRGCYC 2 cut(s) 110, 1668
FspBI CTAG 3 cut(s) 384, 389, 2421
GlaI GCGC 3 cut(s) 1223, 1897, 2382
GsaI CCCAGC 1 cut(s) 497
GsuI CTGGAG 4 cut(s) 533, 669, 1713, 2163
HaeII RGCGCY 1 cut(s) 1899
HaeIII GGCC 8 cut(s) 5, 144, 245, 257, 286, 632, 1334, 1553
HapII CCGG 9 cut(s) 59, 278, 357, 552, 566, 582, 999, 1764, 1787
HhaI GCGC 3 cut(s) 1224, 1898, 2383
Hin6I GCGC 3 cut(s) 1222, 1896, 2381
HinP1I GCGC 3 cut(s) 1222, 1896, 2381
HincII GTYRAC 3 cut(s) 43, 726, 1030
HindII GTYRAC 3 cut(s) 43, 726, 1030
HindIII AAGCTT 1 cut(s) 1019
HinfI GANTC 8 cut(s) 205, 557, 651, 1036, 1637, 1695, 1983, 2111
HpaII CCGG 9 cut(s) 59, 278, 357, 552, 566, 582, 999, 1764, 1787
Hpy166II GTNNAC 5 cut(s) 43, 655, 726, 1030, 2272
Hpy188I TCNGA 6 cut(s) 112, 315, 378, 1041, 1405, 1855
Hpy8I GTNNAC 5 cut(s) 43, 655, 726, 1030, 2272
Hpy99I CGWCG 5 cut(s) 44, 299, 1031, 1217, 2060
HpyAV CCTTC 8 cut(s) 88, 1169, 1213, 1324, 1569, 1614, 1625, 2261
HpyCH4III ACNGT 7 cut(s) 880, 1027, 1078, 1364, 1399, 1484, 2179
HpyCH4IV ACGT 2 cut(s) 24, 39
HpySE526I ACGT 2 cut(s) 24, 39
HspAI GCGC 3 cut(s) 1222, 1896, 2381
Kpn2I TCCGGA 1 cut(s) 277
KpnI GGTACC 2 cut(s) 455, 2355
KroI GCCGGC 1 cut(s) 356
KroNI GCCGGC 1 cut(s) 358
Ksp22I TGATCA 4 cut(s) 315, 1533, 2413, 2457
LmnI GCTCC 3 cut(s) 758, 1285, 2325
MaeI CTAG 3 cut(s) 384, 389, 2421
MaeII ACGT 2 cut(s) 24, 39
MfeI CAATTG 2 cut(s) 1125, 1137
MflI RGATCY 2 cut(s) 129, 602
MhlI GDGCHC 9 cut(s) 110, 122, 846, 1282, 1309, 1507, 1592, 1668, 1710
MlsI TGGCCA 5 cut(s) 5, 245, 257, 286, 1553
MluCI AATT 7 cut(s) 444, 1125, 1137, 1293, 1650, 1936, 2431
MluNI TGGCCA 5 cut(s) 5, 245, 257, 286, 1553
MlyI GAGTC 5 cut(s) 660, 1045, 1646, 1704, 2105
MmeI TCCRAC 1 cut(s) 745
Mox20I TGGCCA 5 cut(s) 5, 245, 257, 286, 1553
MroI TCCGGA 1 cut(s) 277
MroNI GCCGGC 1 cut(s) 356
MscI TGGCCA 5 cut(s) 5, 245, 257, 286, 1553
MseI TTAA 6 cut(s) 527, 1311, 1527, 1725, 2259, 2445
MslI CAYNNNNRTG 9 cut(s) 869, 1482, 1595, 1713, 1752, 1817, 2177, 2309, 2360
Msp20I TGGCCA 5 cut(s) 5, 245, 257, 286, 1553
MspA1I CMGCKG 3 cut(s) 82, 497, 2267
MspCI CTTAAG 1 cut(s) 1310
MspI CCGG 9 cut(s) 59, 278, 357, 552, 566, 582, 999, 1764, 1787
MunI CAATTG 2 cut(s) 1125, 1137
Mva1269I GAATGC 1 cut(s) 2286
NaeI GCCGGC 1 cut(s) 358
NciI CCSGG 4 cut(s) 59, 582, 583, 1787
NcoI CCATGG 1 cut(s) 894
NdeI CATATG 1 cut(s) 2003
NgoMIV GCCGGC 1 cut(s) 356
NlaIV GGNNCC 9 cut(s) 131, 453, 578, 931, 1003, 1591, 2062, 2158, 2353
NmuCI GTSAC 8 cut(s) 568, 707, 889, 918, 989, 1154, 1541, 2311
NspI RCATGY 2 cut(s) 612, 1600
OliI CACNNNNGTG 1 cut(s) 1752
PaeR7I CTCGAG 1 cut(s) 155
PagI TCATGA 2 cut(s) 2067, 2172
PciI ACATGT 1 cut(s) 1596
PctI GAATGC 1 cut(s) 2286
PdiI GCCGGC 1 cut(s) 358
PfeI GAWTC 3 cut(s) 205, 557, 1983
PflFI GACNNNGTC 1 cut(s) 293
PfoI TCCNGGA 2 cut(s) 510, 2114
PinAI ACCGGT 2 cut(s) 551, 565
PleI GAGTC 5 cut(s) 659, 1044, 1645, 1703, 2105
PpsI GAGTC 5 cut(s) 659, 1044, 1645, 1703, 2105
PpuMI RGGWCCY 1 cut(s) 2248
PscI ACATGT 1 cut(s) 1596
PshAI GACNNNNGTC 2 cut(s) 1035, 2180
Psp124BI GAGCTC 2 cut(s) 110, 1668
Psp5II RGGWCCY 1 cut(s) 2248
PspEI GGTNACC 2 cut(s) 568, 1541
PspFI CCCAGC 1 cut(s) 493
PspN4I GGNNCC 9 cut(s) 131, 453, 578, 931, 1003, 1591, 2062, 2158, 2353
PspPI GGNCC 5 cut(s) 143, 1333, 2061, 2248, 2331
PspPPI RGGWCCY 1 cut(s) 2248
PspXI VCTCGAGB 1 cut(s) 155
PstI CTGCAG 1 cut(s) 1737
PsuI RGATCY 2 cut(s) 129, 602
PsyI GACNNNGTC 1 cut(s) 293
PvuII CAGCTG 3 cut(s) 82, 497, 2267
RsaI GTAC 5 cut(s) 453, 794, 1191, 1863, 2353
RsaNI GTAC 5 cut(s) 452, 793, 1190, 1862, 2352
RseI CAYNNNNRTG 9 cut(s) 869, 1482, 1595, 1713, 1752, 1817, 2177, 2309, 2360
SacI GAGCTC 2 cut(s) 110, 1668
SalI GTCGAC 2 cut(s) 41, 1028
SaqAI TTAA 6 cut(s) 527, 1311, 1527, 1725, 2259, 2445
Sau96I GGNCC 5 cut(s) 143, 1333, 2061, 2248, 2331
SchI GAGTC 5 cut(s) 660, 1045, 1646, 1704, 2105
SduI GDGCHC 9 cut(s) 110, 122, 846, 1282, 1309, 1507, 1592, 1668, 1710
SfcI CTRYAG 1 cut(s) 1733
Sfr274I CTCGAG 1 cut(s) 155
SgrAI CRCCGGYG 1 cut(s) 551
SinI GGWCC 3 cut(s) 2061, 2248, 2331
SlaI CTCGAG 1 cut(s) 155
SmaI CCCGGG 1 cut(s) 583
SmiMI CAYNNNNRTG 9 cut(s) 869, 1482, 1595, 1713, 1752, 1817, 2177, 2309, 2360
SmlI CTYRAG 7 cut(s) 47, 155, 182, 1310, 1508, 1793, 2123
SmoI CTYRAG 7 cut(s) 47, 155, 182, 1310, 1508, 1793, 2123
Sse9I AATT 7 cut(s) 444, 1125, 1137, 1293, 1650, 1936, 2431
SsiI CCGC 3 cut(s) 371, 913, 1498
SspI AATATT 1 cut(s) 2226
SspMI CTAG 3 cut(s) 384, 389, 2421
SstI GAGCTC 2 cut(s) 110, 1668
StyI CCWWGG 6 cut(s) 402, 894, 1161, 1413, 1472, 2152
TaaI ACNGT 7 cut(s) 880, 1027, 1078, 1364, 1399, 1484, 2179
TaiI ACGT 2 cut(s) 27, 42
TaqI TCGA 9 cut(s) 42, 75, 92, 156, 342, 1029, 1215, 1242, 1573
TasI AATT 7 cut(s) 444, 1125, 1137, 1293, 1650, 1936, 2431
TatI WGTACW 1 cut(s) 1861
TauI GCSGC 1 cut(s) 374
TfiI GAWTC 3 cut(s) 205, 557, 1983
Tru1I TTAA 6 cut(s) 527, 1311, 1527, 1725, 2259, 2445
Tru9I TTAA 6 cut(s) 527, 1311, 1527, 1725, 2259, 2445
TscAI CASTG 7 cut(s) 994, 1083, 1189, 1489, 1543, 1824, 2146
TseFI GTSAC 8 cut(s) 568, 707, 889, 918, 989, 1154, 1541, 2311
Tsp45I GTSAC 8 cut(s) 568, 707, 889, 918, 989, 1154, 1541, 2311
TspGWI ACGGA 2 cut(s) 877, 1958
TspMI CCCGGG 1 cut(s) 581
TspRI CASTG 7 cut(s) 994, 1083, 1189, 1489, 1543, 1824, 2146
Tth111I GACNNNGTC 1 cut(s) 293
Vha464I CTTAAG 1 cut(s) 1310
VpaK11BI GGWCC 3 cut(s) 2061, 2248, 2331
XapI RAATTY 2 cut(s) 444, 2431
XceI RCATGY 2 cut(s) 612, 1600
XcmI CCANNNNNNNNNTGG 3 cut(s) 662, 961, 1553
XhoI CTCGAG 1 cut(s) 155
XmaI CCCGGG 1 cut(s) 581
XmiI GTMKAC 3 cut(s) 42, 1029, 2271
XspI CTAG 3 cut(s) 384, 389, 2421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.