Rroxscaffold_2G00154660

ATPase 8, plasma

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011636
Physical Location & Seq
Reverse (-)
91199907 .. 91203603
3697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00154660.1

Sequence Viewer

Length: 2292 bp
ATGGCCAATATCTCCTTAGAGGACGTGAAAAAGGAGAACGTCGACCTTGAGAAAATCCCGGTGGAGGAAGTGTTCGAGCAGCTGAAATGCTCGAAGGAGGGGTTGAGCTCAGAGGAGGGCACGAAGAGGATCCAGGTGTTACGGGATGGAAAATGGGGTGAGCAAGAGGCAGAAATTTTGGTACCAGGGGATGTGATTAGCATCAAGTTGGGAGATATAGTCCCAGCTGATGCTCGTCTCCTGGAGGGCGATCCCTTAAAGATTGACCAAGCTGCTCTCACTGGTGAATCCCTACCGGTCACCAGGCACCCAGGGGATGAGGTCTTCTCTGGATCTACATGCAAGCAGGGCGAGATTGAGGCCGTTGTAATCGCAACTGGAGTCCACACTTTCTTTGGCAAGGCTGCACACTTGGTGGATAGCACCAACCAAGTTGGTCACTTCCAAAAGGTGTTGACATCCATTGGTAACTTCTGCATCTGCTCCATTGGAGTTGGAATGGTGATTGAGATACTGGTGATGTACCCCATTCAGCACAGGGCATATAGAGATGGTATTGACAATCTGTTGGTGCTCCTGATCGGAGGTATCCCCATTGCCATGCCAACGGTCTTATCCGTGACCATGGCTATTGGGTCTCACCGCTTGTCACAGCAAGGTGCCATCACCAAGAGGATGACAGCCATTGAAGAAATGGCAGGAATGGATGTCCTTTGCAGTGACAAGACCGGAACCCTCACCCTCAACAAGCTTACCGTGTTTCCTAAGAACATGGACAAAGACACAGTGGTCTTGCTCTCTGCCAGGGCTTCCAGGGTCGAAAACCAGGACGCAATTGATGCCTCAATTGTTGGGATGCTAAGTGACCCCAAGGAGGCAAGAGCAGGGATCACTGAGGTACATTTCCTGCCCTTCAACCCTGTCGACAAGCGCACAGCAATCACCTACATCGATAGCAATGGCGACTGGCACAGATGCAGCAAGGGTGCTCCTGAGCAAATAATTGACCTATGTGCCCTTAAGGGAGAAACGAAGAAGAAGGCCCATGAAGTCATTGACAACTATGCTAACCGTGGTCTTCGTTCATTGGGAGTCGCACGACAGACAGTATCAGAGAAGACCAAGGAAAGTGCTGGTGATGAATGGGAGTTTGTGGGTCTATTGCCTCTCTTTGACCCTCCAAGGCATGACAGTGCTGAGACTATCCGCCGTGCTCTTGAGCTTGGTGTCAATGTTAAGATGATCACTGGTGACCAACTGGCCATTGGAAAAGAGACTGGTCGAAGGCTTGGCATGGGCACCAACATGTATCCTTCATCTACCCTTCTTGGCGAAAGCAAGGACGAGTCCATTGCTTCAATTCCTGTTGATGAGCTCATTGAAAAGGCTGATGGATTTGCTGGAGTCTTCCCTGAGCACAAGTATGAGATTGTTAAGAGACTGCAGGAGAGGAAGCACATCTGTGGTATGACCGGAGATGGTGTGAATGATGCCCCGGCTCTCAAGAGAGCAGACATTGGCATCGCAGTGGATGATGCAACTGATGCAGCCAGGAGTGCCTCGGACATTGTACTGACTGAGCCAGGACTAAGTGTTATTGTGAGCGCCGTCTTGACAAGCAGAGCCATCTTCCAGAGGATGAAGAACTATACCATTTATGCTGTTTCCATCACAATCCGTATTGTGCTGGGATTCATGCTTCTTGCTCTCATATGGAAATATGACTTTCCACCATTCATGGTCTTGGTCATTGCAGTCTTGAACGACGGGACCATCATGACCATCTCTAAGGATAGAGTGAAGCCATCTCCTCTGCCTGACTCCTGGAAACTCAAGGAAATCTTCGCCACTGGAGTTGTCCTTGGTTCCTACATGGCTGTCATGACTGTGGTCTTCTTCTGGCTTGCACACAGCACTCTCTTTTTCTCTAAAATATTTGGTGTGAGACCTATTGAGGACCTTCAGCTTAACTCAGCCGTCTACCTCCAAGTGAGCATTCTCAGTCAAGCACTCATCTTTGTGACCCGATCAAGGAGCTGGTCCTTTGTTGAACGCCCTGGTACCATGCTTATGGTTGCCTTTCTTGCAGCGCAGTTGGTGGCTACCCTCATTGCTGTATATTGTGACTGGGGCTTTGCCAAAATCAAGGGTATCGGATGGGGATGGGCAGGCGCTATCTGGGTCTACAGCCTTGTCACCTATTTCCCTCTCGATATCTTCAAGTTCCTGATCCGTTATGCATTGAGTGGCCATGCCTGGGATAATGTTGTCCAGAACAAGGTTTGTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

763

Amino Acids

83.7

Weight (kDa)

5.8

Isoelectric Point (pI)

34.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E1-E2_ATPase PF00122 43 - 139 1.8e-28 P-type ATPase actuator domain
Hydrolase PF00702 235 - 505 1.9e-13 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 290 - 396 4.1e-07 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011636)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07560 AT3G42640
fragaria_vesca FvH4_1g01450 FvH4_1g01450
malus_domestica MD02G1011600.v1.1
prunus_persica Prupe.7G258000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0086371
rosa_laevigata RLG00000015753
rosa_multiflora Rmu_co8318751.1_g000001 Rmu_sc0003572.1_g000001 Rmu_sc0005127.1_g000010
rosa_roxburghii Rroxscaffold_2G00154660
rosa_rugosa Rorug01G0466900 Rorug01G0467000
rosa_samantha Rh2AG017100 Rh2BG016900 Rh2CG017900 Rh2DG018600
rosa_wichuraiana Rw2G001450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 788
Acc65I GGTACC 2 cut(s) 181, 2060
AccB1I GGYRCC 5 cut(s) 181, 306, 659, 1298, 2060
AccI GTMKAC 4 cut(s) 42, 924, 1980, 2184
AciI CCGC 2 cut(s) 643, 1207
AclWI GGATC 6 cut(s) 124, 137, 245, 340, 896, 2224
AcoI YGGCCR 3 cut(s) 3, 1260, 2248
AcsI RAATTY 1 cut(s) 174
AcuI CTGAAG 1 cut(s) 1946
AdeI CACNNNGTG 1 cut(s) 415
AfaI GTAC 5 cut(s) 183, 524, 900, 1572, 2062
AfiI CCNNNNNNNGG 5 cut(s) 64, 874, 2031, 2257, 2278
AflII CTTAAG 1 cut(s) 1019
AflIII ACRYGT 1 cut(s) 1305
AgeI ACCGGT 1 cut(s) 295
AgsI TTSAA 7 cut(s) 689, 916, 1359, 1382, 1762, 2051, 2221
AjiI CACGTC 1 cut(s) 25
AjuI GAANNNNNNNTTGG 2 cut(s) 1980, 2012
AleI CACNNNNGTG 1 cut(s) 1461
AloI GAACNNNNNNTCC 2 cut(s) 56, 88
AluBI AGCT 9 cut(s) 82, 108, 227, 272, 751, 1222, 1375, 1966, 2037
AluI AGCT 9 cut(s) 82, 108, 227, 272, 751, 1222, 1375, 1966, 2037
Alw21I GWGCWC 6 cut(s) 110, 576, 991, 1216, 1377, 1419
Alw26I GTCTC 6 cut(s) 242, 642, 1193, 1268, 1432, 1939
AlwI GGATC 6 cut(s) 124, 137, 245, 340, 896, 2224
AoxI GGCC 5 cut(s) 3, 360, 1041, 1260, 2248
ApeKI GCWGC 6 cut(s) 79, 272, 404, 978, 1547, 2087
ApoI RAATTY 1 cut(s) 174
ArsI GACNNNNNNTTYG 2 cut(s) 2117, 2149
AsiGI ACCGGT 1 cut(s) 295
Asp718I GGTACC 2 cut(s) 181, 2060
AspLEI GCGC 4 cut(s) 933, 1607, 2092, 2174
AspS9I GGNCC 4 cut(s) 1042, 1770, 1957, 2040
AsuC2I CCSGG 2 cut(s) 59, 1496
AvaII GGWCC 3 cut(s) 1770, 1957, 2040
BaeGI GKGCMC 3 cut(s) 122, 1018, 1301
BalI TGGCCA 3 cut(s) 5, 1262, 2250
BamHI GGATCC 1 cut(s) 129
BanI GGYRCC 5 cut(s) 181, 306, 659, 1298, 2060
BanII GRGCYC 2 cut(s) 110, 1377
BbsI GAAGAC 5 cut(s) 316, 1070, 1124, 1399, 1885
Bbv12I GWGCWC 6 cut(s) 110, 576, 991, 1216, 1377, 1419
BbvI GCAGC 6 cut(s) 91, 259, 391, 990, 1559, 2099
BceAI ACGGC 4 cut(s) 347, 1194, 1592, 1961
BcgI CGANNNNNNTGC 2 cut(s) 1334, 1368
BciVI GTATCC 2 cut(s) 599, 1320
BclI TGATCA 1 cut(s) 1242
BcnI CCSGG 2 cut(s) 59, 1496
BcoDI GTCTC 6 cut(s) 242, 642, 1193, 1268, 1432, 1939
BfaI CTAG 1 cut(s) 2290
BfmI CTRYAG 2 cut(s) 1442, 2185
BfoI RGCGCY 2 cut(s) 1608, 2175
BfrI CTTAAG 1 cut(s) 1019
BfuI GTATCC 2 cut(s) 599, 1320
BisI GCNGC 6 cut(s) 80, 273, 405, 979, 1548, 2088
BlsI GCNGC 6 cut(s) 81, 274, 406, 980, 1549, 2089
Bme18I GGWCC 3 cut(s) 1770, 1957, 2040
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 4 cut(s) 1042, 1770, 1957, 2040
BmiI GGNNCC 9 cut(s) 131, 183, 308, 661, 733, 1300, 1771, 1867, 2062
BmrI ACTGGG 1 cut(s) 2137
BmuI ACTGGG 1 cut(s) 2137
BoxI GACNNNNGTC 1 cut(s) 1889
BpiI GAAGAC 5 cut(s) 316, 1070, 1124, 1399, 1885
BplI GAGNNNNNCTC 2 cut(s) 311, 343
BpmI CTGGAG 4 cut(s) 263, 399, 1422, 1872
Bpu10I CCTNAGC 2 cut(s) 993, 1413
BpuEI CTTGAG 4 cut(s) 68, 1238, 1487, 1817
BpuMI CCSGG 2 cut(s) 59, 1496
Bsa29I ATCGAT 1 cut(s) 951
BsaBI GATNNNNATC 1 cut(s) 200
BsaI GGTCTC 2 cut(s) 642, 1939
BsaWI WCCGGW 3 cut(s) 295, 728, 1472
BsaXI ACNNNNNCTCC 6 cut(s) 56, 86, 204, 234, 1467, 1497
Bsc4I CCNNNNNNNGG 5 cut(s) 64, 874, 2031, 2257, 2278
Bse118I RCCGGY 1 cut(s) 295
Bse1I ACTGG 9 cut(s) 286, 382, 519, 971, 1252, 1263, 1282, 1855, 2132
Bse3DI GCAATG 5 cut(s) 594, 964, 1350, 1749, 2109
Bse8I GATNNNNATC 1 cut(s) 200
BseCI ATCGAT 1 cut(s) 951
BseJI GATNNNNATC 1 cut(s) 200
BseLI CCNNNNNNNGG 5 cut(s) 64, 874, 2031, 2257, 2278
BseMI GCAATG 5 cut(s) 594, 964, 1350, 1749, 2109
BseMII CTCAG 8 cut(s) 123, 885, 984, 1188, 1404, 1569, 1986, 2014
BseNI ACTGG 9 cut(s) 286, 382, 519, 971, 1252, 1263, 1282, 1855, 2132
BseRI GAGGAG 2 cut(s) 128, 1800
BseSI GKGCMC 3 cut(s) 122, 1018, 1301
BseXI GCAGC 6 cut(s) 91, 259, 391, 990, 1559, 2099
BseYI CCCAGC 2 cut(s) 223, 1687
BsgI GTGCAG 1 cut(s) 390
BshFI GGCC 5 cut(s) 5, 362, 1043, 1262, 2250
BshNI GGYRCC 5 cut(s) 181, 306, 659, 1298, 2060
BshTI ACCGGT 1 cut(s) 295
BshVI ATCGAT 1 cut(s) 951
BsiHKAI GWGCWC 6 cut(s) 110, 576, 991, 1216, 1377, 1419
BsiSI CCGG 5 cut(s) 59, 296, 729, 1473, 1496
BslFI GGGAC 2 cut(s) 206, 1783
BslI CCNNNNNNNGG 5 cut(s) 64, 874, 2031, 2257, 2278
BsmAI GTCTC 6 cut(s) 242, 642, 1193, 1268, 1432, 1939
BsmBI CGTCTC 1 cut(s) 242
BsmFI GGGAC 2 cut(s) 206, 1783
BsmI GAATGC 1 cut(s) 1995
BsnI GGCC 5 cut(s) 5, 362, 1043, 1262, 2250
Bso31I GGTCTC 2 cut(s) 642, 1939
Bsp1286I GDGCHC 9 cut(s) 110, 122, 576, 991, 1018, 1216, 1301, 1377, 1419
Bsp143I GATC 8 cut(s) 129, 250, 332, 579, 888, 1242, 2027, 2229
Bsp19I CCATGG 1 cut(s) 624
BspACI CCGC 2 cut(s) 643, 1207
BspANI GGCC 5 cut(s) 5, 362, 1043, 1262, 2250
BspCNI CTCAG 8 cut(s) 122, 886, 985, 1189, 1405, 1570, 1985, 2013
BspDI ATCGAT 1 cut(s) 951
BspHI TCATGA 2 cut(s) 1776, 1881
BspLI GGNNCC 9 cut(s) 131, 183, 308, 661, 733, 1300, 1771, 1867, 2062
BspMAI CTGCAG 1 cut(s) 1446
BspPI GGATC 6 cut(s) 124, 137, 245, 340, 896, 2224
BspT107I GGYRCC 5 cut(s) 181, 306, 659, 1298, 2060
BspTI CTTAAG 1 cut(s) 1019
BspTNI GGTCTC 2 cut(s) 642, 1939
BsrDI GCAATG 5 cut(s) 594, 964, 1350, 1749, 2109
BsrFI RCCGGY 1 cut(s) 295
BsrI ACTGG 9 cut(s) 286, 382, 519, 971, 1252, 1263, 1282, 1855, 2132
BssAI RCCGGY 1 cut(s) 295
BssMI GATC 8 cut(s) 129, 250, 332, 579, 888, 1242, 2027, 2229
BssT1I CCWWGG 5 cut(s) 624, 870, 1122, 1181, 1861
Bst4CI ACNGT 7 cut(s) 610, 757, 787, 1073, 1108, 1193, 1888
Bst6I CTCTTC 1 cut(s) 119
BstAFI CTTAAG 1 cut(s) 1019
BstAPI GCANNNNNTGC 2 cut(s) 839, 1544
BstC8I GCNNGC 3 cut(s) 344, 1905, 2170
BstDSI CCRYGG 2 cut(s) 624, 1072
BstEII GGTNACC 2 cut(s) 298, 1250
BstH2I RGCGCY 2 cut(s) 1608, 2175
BstHHI GCGC 4 cut(s) 933, 1607, 2092, 2174
BstKTI GATC 8 cut(s) 132, 253, 335, 582, 891, 1245, 2030, 2232
BstMAI GTCTC 6 cut(s) 242, 642, 1193, 1268, 1432, 1939
BstMBI GATC 8 cut(s) 129, 250, 332, 579, 888, 1242, 2027, 2229
BstMWI GCNNNNNNNGC 5 cut(s) 348, 839, 1544, 1556, 2084
BstNSI RCATGY 2 cut(s) 342, 1309
BstPAI GACNNNNGTC 1 cut(s) 1889
BstPI GGTNACC 2 cut(s) 298, 1250
BstSFI CTRYAG 2 cut(s) 1442, 2185
BstSLI GKGCMC 3 cut(s) 122, 1018, 1301
BstV1I GCAGC 6 cut(s) 91, 259, 391, 990, 1559, 2099
BstV2I GAAGAC 5 cut(s) 316, 1070, 1124, 1399, 1885
BstX2I RGATCY 2 cut(s) 129, 332
BstXI CCANNNNNNTGG 1 cut(s) 2071
BstYI RGATCY 2 cut(s) 129, 332
Bsu15I ATCGAT 1 cut(s) 951
BsuI GTATCC 2 cut(s) 599, 1320
BsuRI GGCC 5 cut(s) 5, 362, 1043, 1262, 2250
BsuTUI ATCGAT 1 cut(s) 951
BtgI CCRYGG 2 cut(s) 624, 1072
BtgZI GCGATG 1 cut(s) 1507
BtrI CACGTC 1 cut(s) 25
BtsI GCAGTG 2 cut(s) 724, 1533
BtsIMutI CAGTG 8 cut(s) 279, 724, 792, 891, 1198, 1245, 1533, 1848
Cac8I GCNNGC 3 cut(s) 344, 1905, 2170
CciI TCATGA 2 cut(s) 1776, 1881
CfoI GCGC 4 cut(s) 933, 1607, 2092, 2174
Cfr10I RCCGGY 1 cut(s) 295
Cfr13I GGNCC 4 cut(s) 1042, 1770, 1957, 2040
ClaI ATCGAT 1 cut(s) 951
CseI GACGC 1 cut(s) 839
Csp6I GTAC 5 cut(s) 182, 523, 899, 1571, 2061
CspAI ACCGGT 1 cut(s) 295
CspCI CAANNNNNGTGG 2 cut(s) 1837, 1872
CviQI GTAC 5 cut(s) 182, 523, 899, 1571, 2061
DpnI GATC 8 cut(s) 131, 252, 334, 581, 890, 1244, 2029, 2231
DpnII GATC 8 cut(s) 129, 250, 332, 579, 888, 1242, 2027, 2229
DraIII CACNNNGTG 1 cut(s) 415
DrdI GACNNNNNNGTC 1 cut(s) 788
DseDI GACNNNNNNGTC 1 cut(s) 788
EaeI YGGCCR 3 cut(s) 3, 1260, 2248
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
EciI GGCGGA 1 cut(s) 1196
Ecl136II GAGCTC 2 cut(s) 108, 1375
Eco130I CCWWGG 5 cut(s) 624, 870, 1122, 1181, 1861
Eco24I GRGCYC 2 cut(s) 110, 1377
Eco31I GGTCTC 2 cut(s) 642, 1939
Eco32I GATATC 1 cut(s) 2215
Eco47I GGWCC 3 cut(s) 1770, 1957, 2040
Eco53kI GAGCTC 2 cut(s) 108, 1375
Eco57I CTGAAG 1 cut(s) 1946
Eco91I GGTNACC 2 cut(s) 298, 1250
EcoICRI GAGCTC 2 cut(s) 108, 1375
EcoO109I RGGNCCY 1 cut(s) 1957
EcoO65I GGTNACC 2 cut(s) 298, 1250
EcoRV GATATC 1 cut(s) 2215
EcoT14I CCWWGG 5 cut(s) 624, 870, 1122, 1181, 1861
EcoT22I ATGCAT 1 cut(s) 2242
EcoT38I GRGCYC 2 cut(s) 110, 1377
ErhI CCWWGG 5 cut(s) 624, 870, 1122, 1181, 1861
Esp3I CGTCTC 1 cut(s) 242
FalI AAGNNNNNCTT 2 cut(s) 1826, 1858
FaqI GGGAC 2 cut(s) 206, 1783
FauNDI CATATG 1 cut(s) 1712
FbaI TGATCA 1 cut(s) 1242
FblI GTMKAC 4 cut(s) 42, 924, 1980, 2184
Fnu4HI GCNGC 6 cut(s) 80, 273, 405, 979, 1548, 2088
FriOI GRGCYC 2 cut(s) 110, 1377
Fsp4HI GCNGC 6 cut(s) 80, 273, 405, 979, 1548, 2088
FspBI CTAG 1 cut(s) 2290
GlaI GCGC 4 cut(s) 932, 1606, 2091, 2173
GluI GCNGC 6 cut(s) 80, 273, 405, 979, 1548, 2088
GsaI CCCAGC 2 cut(s) 227, 1691
GsuI CTGGAG 4 cut(s) 263, 399, 1422, 1872
HaeII RGCGCY 2 cut(s) 1608, 2175
HaeIII GGCC 5 cut(s) 5, 362, 1043, 1262, 2250
HapII CCGG 5 cut(s) 59, 296, 729, 1473, 1496
HgaI GACGC 1 cut(s) 839
HhaI GCGC 4 cut(s) 933, 1607, 2092, 2174
Hin6I GCGC 4 cut(s) 931, 1605, 2090, 2172
HinP1I GCGC 4 cut(s) 931, 1605, 2090, 2172
HincII GTYRAC 3 cut(s) 43, 456, 925
HindII GTYRAC 3 cut(s) 43, 456, 925
HindIII AAGCTT 1 cut(s) 749
HinfI GANTC 7 cut(s) 287, 381, 1092, 1346, 1404, 1692, 1820
HpaII CCGG 5 cut(s) 59, 296, 729, 1473, 1496
Hpy166II GTNNAC 6 cut(s) 43, 385, 456, 925, 1981, 2185
Hpy188I TCNGA 5 cut(s) 112, 584, 1114, 1564, 2156
Hpy8I GTNNAC 6 cut(s) 43, 385, 456, 925, 1981, 2185
Hpy99I CGWCG 2 cut(s) 44, 1769
HpyAV CCTTC 7 cut(s) 88, 922, 1033, 1278, 1323, 1334, 1970
HpyCH4III ACNGT 7 cut(s) 610, 757, 787, 1073, 1108, 1193, 1888
HpyCH4IV ACGT 2 cut(s) 24, 39
HpyF10VI GCNNNNNNNGC 5 cut(s) 348, 839, 1544, 1556, 2084
HpySE526I ACGT 2 cut(s) 24, 39
HspAI GCGC 4 cut(s) 931, 1605, 2090, 2172
KpnI GGTACC 2 cut(s) 185, 2064
Ksp22I TGATCA 1 cut(s) 1242
Kzo9I GATC 8 cut(s) 129, 250, 332, 579, 888, 1242, 2027, 2229
LmnI GCTCC 4 cut(s) 488, 579, 994, 2034
Lsp1109I GCAGC 6 cut(s) 91, 259, 391, 990, 1559, 2099
MaeI CTAG 1 cut(s) 2290
MaeII ACGT 2 cut(s) 24, 39
MalI GATC 8 cut(s) 131, 252, 334, 581, 890, 1244, 2029, 2231
MboI GATC 8 cut(s) 129, 250, 332, 579, 888, 1242, 2027, 2229
MfeI CAATTG 2 cut(s) 834, 846
MflI RGATCY 2 cut(s) 129, 332
MhlI GDGCHC 9 cut(s) 110, 122, 576, 991, 1018, 1216, 1301, 1377, 1419
MlsI TGGCCA 3 cut(s) 5, 1262, 2250
MluCI AATT 5 cut(s) 174, 834, 846, 1002, 1359
MluNI TGGCCA 3 cut(s) 5, 1262, 2250
MlyI GAGTC 5 cut(s) 390, 1101, 1355, 1413, 1814
MmeI TCCRAC 1 cut(s) 475
Mox20I TGGCCA 3 cut(s) 5, 1262, 2250
Mph1103I ATGCAT 1 cut(s) 2242
MscI TGGCCA 3 cut(s) 5, 1262, 2250
MseI TTAA 5 cut(s) 257, 1020, 1236, 1434, 1968
MslI CAYNNNNRTG 9 cut(s) 599, 1191, 1304, 1422, 1461, 1526, 1886, 2018, 2069
Msp20I TGGCCA 3 cut(s) 5, 1262, 2250
MspA1I CMGCKG 2 cut(s) 82, 227
MspCI CTTAAG 1 cut(s) 1019
MspI CCGG 5 cut(s) 59, 296, 729, 1473, 1496
MunI CAATTG 2 cut(s) 834, 846
Mva1269I GAATGC 1 cut(s) 1995
MwoI GCNNNNNNNGC 5 cut(s) 348, 839, 1544, 1556, 2084
NciI CCSGG 2 cut(s) 59, 1496
NcoI CCATGG 1 cut(s) 624
NdeI CATATG 1 cut(s) 1712
NdeII GATC 8 cut(s) 129, 250, 332, 579, 888, 1242, 2027, 2229
NlaIV GGNNCC 9 cut(s) 131, 183, 308, 661, 733, 1300, 1771, 1867, 2062
NsiI ATGCAT 1 cut(s) 2242
NspI RCATGY 2 cut(s) 342, 1309
OliI CACNNNNGTG 1 cut(s) 1461
PagI TCATGA 2 cut(s) 1776, 1881
PasI CCCWGGG 1 cut(s) 311
PciI ACATGT 1 cut(s) 1305
PctI GAATGC 1 cut(s) 1995
PfeI GAWTC 2 cut(s) 287, 1692
PfoI TCCNGGA 2 cut(s) 240, 1823
PinAI ACCGGT 1 cut(s) 295
PkrI GCNGC 6 cut(s) 81, 274, 406, 980, 1549, 2089
PleI GAGTC 5 cut(s) 389, 1100, 1354, 1412, 1814
PpsI GAGTC 5 cut(s) 389, 1100, 1354, 1412, 1814
PpuMI RGGWCCY 1 cut(s) 1957
PscI ACATGT 1 cut(s) 1305
PshAI GACNNNNGTC 1 cut(s) 1889
Psp124BI GAGCTC 2 cut(s) 110, 1377
Psp5II RGGWCCY 1 cut(s) 1957
PspEI GGTNACC 2 cut(s) 298, 1250
PspFI CCCAGC 2 cut(s) 223, 1687
PspN4I GGNNCC 9 cut(s) 131, 183, 308, 661, 733, 1300, 1771, 1867, 2062
PspPI GGNCC 4 cut(s) 1042, 1770, 1957, 2040
PspPPI RGGWCCY 1 cut(s) 1957
PstI CTGCAG 1 cut(s) 1446
PsuI RGATCY 2 cut(s) 129, 332
PvuII CAGCTG 2 cut(s) 82, 227
RsaI GTAC 5 cut(s) 183, 524, 900, 1572, 2062
RsaNI GTAC 5 cut(s) 182, 523, 899, 1571, 2061
RseI CAYNNNNRTG 9 cut(s) 599, 1191, 1304, 1422, 1461, 1526, 1886, 2018, 2069
SacI GAGCTC 2 cut(s) 110, 1377
SalI GTCGAC 2 cut(s) 41, 923
SaqAI TTAA 5 cut(s) 257, 1020, 1236, 1434, 1968
SatI GCNGC 6 cut(s) 80, 273, 405, 979, 1548, 2088
Sau3AI GATC 8 cut(s) 129, 250, 332, 579, 888, 1242, 2027, 2229
Sau96I GGNCC 4 cut(s) 1042, 1770, 1957, 2040
SchI GAGTC 5 cut(s) 390, 1101, 1355, 1413, 1814
SduI GDGCHC 9 cut(s) 110, 122, 576, 991, 1018, 1216, 1301, 1377, 1419
SfcI CTRYAG 2 cut(s) 1442, 2185
SinI GGWCC 3 cut(s) 1770, 1957, 2040
SmiMI CAYNNNNRTG 9 cut(s) 599, 1191, 1304, 1422, 1461, 1526, 1886, 2018, 2069
SmlI CTYRAG 5 cut(s) 47, 1019, 1217, 1502, 1832
SmoI CTYRAG 5 cut(s) 47, 1019, 1217, 1502, 1832
Sse9I AATT 5 cut(s) 174, 834, 846, 1002, 1359
SsiI CCGC 2 cut(s) 643, 1207
SspI AATATT 1 cut(s) 1935
SspMI CTAG 1 cut(s) 2290
SstI GAGCTC 2 cut(s) 110, 1377
StyI CCWWGG 5 cut(s) 624, 870, 1122, 1181, 1861
TaaI ACNGT 7 cut(s) 610, 757, 787, 1073, 1108, 1193, 1888
TaiI ACGT 2 cut(s) 27, 42
TaqI TCGA 8 cut(s) 42, 75, 92, 819, 924, 951, 1282, 2211
TasI AATT 5 cut(s) 174, 834, 846, 1002, 1359
TatI WGTACW 1 cut(s) 1570
TfiI GAWTC 2 cut(s) 287, 1692
Tru1I TTAA 5 cut(s) 257, 1020, 1236, 1434, 1968
Tru9I TTAA 5 cut(s) 257, 1020, 1236, 1434, 1968
TscAI CASTG 8 cut(s) 286, 724, 792, 898, 1198, 1252, 1533, 1855
TseI GCWGC 6 cut(s) 79, 272, 404, 978, 1547, 2087
TspDTI ATGAA 7 cut(s) 1062, 1074, 1155, 1305, 1655, 1684, 1726
TspGWI ACGGA 3 cut(s) 607, 1667, 2222
TspRI CASTG 8 cut(s) 286, 724, 792, 898, 1198, 1252, 1533, 1855
Vha464I CTTAAG 1 cut(s) 1019
VpaK11BI GGWCC 3 cut(s) 1770, 1957, 2040
XapI RAATTY 1 cut(s) 174
XceI RCATGY 2 cut(s) 342, 1309
XcmI CCANNNNNNNNNTGG 3 cut(s) 392, 691, 1262
XmiI GTMKAC 4 cut(s) 42, 924, 1980, 2184
XspI CTAG 1 cut(s) 2290
Zsp2I ATGCAT 1 cut(s) 2242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.