MD02G1011600.v1.1

ATPase 8, plasma

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011636
Physical Location & Seq
Forward (+)
740009 .. 744563
4555 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1011600.v1.1.491

Sequence Viewer

Length: 2844 bp
ATGGCCAACATCTCCTTAGAAGACGTCAAGAACGAGAACGTCGACCTCGAGCGAATCCCCGTTGAGGAAGTGTTCGAGCAGCTGAAATGCACGAAAAGTGGATTGACGGCCGAGGAAGGCCAGAAGCGGCTGGAGATCTTCGGCCCGAACAAGCTTGAGGAGAAGAAGGAGTGCAAGGTCCTTAAGTTCCTGGGATTTATGTGGAATCCTCTATCATGGGTCATGGAGTGCGCTGCTATCATGGCCCTGGTTTTGGCCAATGGAGGGGGCAAGCCAACTGACTGGCCAGACTTCGTCGGTATTATGGTGTTGTTGCTCATCAACTCAACCATCAGTTTCATCGAGGAAAACAACGCCGGCAATGCTGCGGCAGCTCTGATGGCTAGTCTTGCCCCCAAGACTAAGGTCTTGAGGGATGGAAAGTGGGGGGAGCAAGAGGCAGAAATCCTGGTGCCAGGAGATGTGATCAGCATCAAGTTGGGAGATATCGTCCCAGCTGATGCGCGTCTCTTGGAAGGTGATCCTCTCAAGATTGACCAGGCTGCTCTCACCGGTGAGTCCCTTCCCGTTACCAGGAACCCAGGAGATGAGGTATTCTCTGGCTCTACCTGCAAGCAGGGTGAGATCGAAGCTGTTGTCATTGCTACCGGTGTCCATACATTCTTCGGAAAGGCTGCTCACCTTGTGGATAGCACCAACCAAGTGGGTCACTTCCAAAAGGTGTTGACATCCATCGGAAACTTCTGCATATGCTCCATTGCTGTGGGAATGGTAATTGAGATCCTGGTGATGTATCCAATTCAGCATAGGGCATATAGAGATGGTATTGACAACCTGCTGGTGCTTCTGATCGGAGGTATCCCCATTGCCATGCCCACGGTCTTGTCCGTGACCATGGCTATTGGGTCTCACCGCCTTTCACAGCAAGGCGCCATCACCAAGAGGATGACAGCCATTGAAGAGATGGCTGGGATGGACGTTCTTTGCAGTGATAAGACCGGTACCCTCACCCTCAACAAGCTTACCGTCGATAAGAGTCTAATTGAGGTGTTCCCGAAGAACGTGGATAAGGACGGCGTGGTGTTGCTCTCTGCTAGGGCCTCCAGGGTCGAAAACCAGGACGCCATTGATGCCTCGATTGTTAATATGTTGGCCGACCCCAAGGAGGCAAGAGCAGGAATCACTGAGGTGCATTTCCTGCCCTTCAATCCCGTCGACAAGCGCACAGCAATCACCTACATCGATGCCAATGGCGACTGGCACAGATGCAGCAAGGGTGCACCTGAGCAAATCATTGACCTTTGCGATCTCAAGGGAGAAACAAGGAAGAAGGCCCATGCTGTCATTGACAACTACGCAGAACGCGGTCTCCGCTCATTGGGAGTTGCTCGACAGACAGTTCCTGAGAAGACCAAGGAGAGCGCCGGCAGTCCATGGGAGTTTGTCGGTTTACTGCCTCTCTTTGATCCTCCCAGGCACGACAGTGCTGAGACTATCCGCCGTGCTCTTGAGCTCGGTGTCAACGTTAAGATGATCACTGGTGACCAACTTGCCATTGGGAAAGAGACCGGTCGCAGGCTCGGCATGGGAACTAACATGTATCCGTCATCCACCCTTCTAGGACAGAGCAAGGACGAGTCCATTGCCTCCATGCCTGTTGATGAGCTCATCGAAAAGGCTGATGGTTTCGCCGGAGTCTTTCCTGAGCACAAGTATGAGATTGTAAAGAAGCTACAGGAGAGGAAGCACATCTGCGGGATGACTGGAGATGGTGTGAATGATGCCCCGGCGCTCAAGAGGGCGGACATTGGTATTGCCGTGGCAGATGCAACTGACGCAGCCAGGAGCGCGTCGGACATTGTCCTGACAGAGCCAGGGCTGAGTGTGATTGTGAGTGCCGTCTTGACAAGCAGAGCCATCTTCCAGAGGATGAAGAACTATACAATCTACGCTGTTTCCATCACAATCCGTATTGTGGTGGGATTCATGCTCGTTGCTCTCATCTGGAAGTTCGACTTCTCGCCTTTCATGGTCTTGGTTATCGCTGTCCTGAACGATGGGACTATCATGACCATCTCCAAGGACAGAGTGAAGCCGTCTCCTCTCCCTGACTCATGGAAGCTCAAGGAAATCTTCGCCACTGGCGTTGTCCTTGGAACCTACATGGCTGTCATGACCGTGGTCTTCTTCTGGCTTGCAAATAGCACCGACTTCTTCGGAAAACATTTTGGAGTGCACAATATTTCAGACAACCCGAAACAGCTTAACTCGGCTATCTACCTACAAGTGAGCATCATCAGCCAAGCGCTCATCTTCGTGACTCGATCTAGGAGCTGGTCCTTCCTCGAACGCCCTGGTCTCATGCTTTTGGTCGCCTTTCTTGCAGCTCAGTTGGTGGCCACGCTCATCGCTGTGTACTGCAGTTGGGGCTTTGCCAGAATCGAAGGCGTTGGATGGGGATGGGCCGGTGTCATCTGGATCTACAGCATCGTCACCTACTTCCCCCTCGATATCTTCAAGTTCATTATCCGCTACGCATTGACCGGCAAGGCCTGGGATACCATTGTCCAGAACAAGACTGCCTTCACATCAAACAATGACTACGGAAAGGGGGCAAGAGAAGCGCAATGGGCGTCAGCTCAGCGTTCGCTCAACGGTCTCCAGCCTCCGGAAGCATTGTTCCACGGCAACCACGGGGAGCAGGCGGAGCTAGCTGAGCAGGCCAAGAGGCGCGCGGAAGTCGCTAGACTGAGGGAACTGCACACGTTAAAGGGGCACGTCGAATCAGTGGTTAAGCTGAAGGGGCTAGACATTGACACCATCCAACAACACTACACTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

948

Amino Acids

103.68

Weight (kDa)

6.05

Isoelectric Point (pI)

34.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 18 - 81 8.2e-14 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 130 - 229 3.1e-29 P-type ATPase actuator domain
Hydrolase PF00702 325 - 602 2.5e-13 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 386 - 493 3.2e-06 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011636)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07560 AT3G42640
fragaria_vesca FvH4_1g01450 FvH4_1g01450
malus_domestica MD02G1011600.v1.1
prunus_persica Prupe.7G258000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0086371
rosa_laevigata RLG00000015753
rosa_multiflora Rmu_co8318751.1_g000001 Rmu_sc0003572.1_g000001 Rmu_sc0005127.1_g000010
rosa_roxburghii Rroxscaffold_2G00154660
rosa_rugosa Rorug01G0466900 Rorug01G0467000
rosa_samantha Rh2AG017100 Rh2BG016900 Rh2CG017900 Rh2DG018600
rosa_wichuraiana Rw2G001450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 27
Acc36I ACCTGC 2 cut(s) 617, 843
Acc65I GGTACC 1 cut(s) 1001
AccB1I GGYRCC 3 cut(s) 451, 929, 1001
AccBSI CCGCTC 1 cut(s) 1374
AccI GTMKAC 2 cut(s) 42, 1215
AccII CGCG 5 cut(s) 505, 1365, 1850, 2733, 2735
AccIII TCCGGA 1 cut(s) 2668
AclI AACGTT 1 cut(s) 1524
AclWI GGATC 4 cut(s) 515, 775, 1460, 2486
AcoI YGGCCR 6 cut(s) 3, 108, 255, 284, 1152, 2397
AcuI CTGAAG 1 cut(s) 2819
AcyI GRCGYC 4 cut(s) 24, 930, 1122, 2633
AdeI CACNNNGTG 1 cut(s) 685
AfaI GTAC 2 cut(s) 1003, 2417
AfeI AGCGCT 1 cut(s) 2307
AfiI CCNNNNNNNGG 9 cut(s) 64, 253, 264, 573, 1165, 1378, 1575, 1975, 2668
AflII CTTAAG 1 cut(s) 182
AflIII ACRYGT 2 cut(s) 1596, 2763
AgeI ACCGGT 4 cut(s) 551, 647, 998, 1568
AgsI TTSAA 3 cut(s) 959, 1207, 2518
AjiI CACGTC 1 cut(s) 2779
AleI CACNNNNGTG 2 cut(s) 1187, 1482
AloI GAACNNNNNNTCC 4 cut(s) 1353, 1385, 2663, 2695
Alw21I GWGCWC 6 cut(s) 1282, 1507, 1515, 1668, 1710, 2238
Alw26I GTCTC 8 cut(s) 512, 912, 1373, 1484, 1559, 2103, 2363, 2663
Alw44I GTGCAC 2 cut(s) 1278, 2234
AlwI GGATC 4 cut(s) 515, 775, 1460, 2486
AlwNI CAGNNNCTG 1 cut(s) 1403
Ama87I CYCGRG 1 cut(s) 47
Aor13HI TCCGGA 1 cut(s) 2668
Aor51HI AGCGCT 1 cut(s) 2307
ApaLI GTGCAC 2 cut(s) 1278, 2234
ApeKI GCWGC 9 cut(s) 79, 233, 365, 371, 542, 674, 1269, 1838, 2384
ArsI GACNNNNNNTTYG 2 cut(s) 621, 653
AsiGI ACCGGT 4 cut(s) 551, 647, 998, 1568
Asp718I GGTACC 1 cut(s) 1001
AspS9I GGNCC 7 cut(s) 143, 178, 244, 1098, 1333, 2337, 2463
AsuC2I CCSGG 1 cut(s) 1787
AsuNHI GCTAGC 1 cut(s) 2710
AvaI CYCGRG 1 cut(s) 47
AvaII GGWCC 2 cut(s) 178, 2337
BaeGI GKGCMC 3 cut(s) 1282, 2238, 2778
BaeI ACNNNNGTAYC 2 cut(s) 2550, 2583
BalI TGGCCA 4 cut(s) 5, 257, 286, 2399
BanI GGYRCC 3 cut(s) 451, 929, 1001
BanII GRGCYC 2 cut(s) 1515, 1668
BbsI GAAGAC 3 cut(s) 27, 1415, 2176
Bbv12I GWGCWC 6 cut(s) 1282, 1507, 1515, 1668, 1710, 2238
BbvI GCAGC 9 cut(s) 91, 220, 352, 383, 529, 661, 1281, 1850, 2396
BceAI ACGGC 7 cut(s) 123, 1090, 1485, 1802, 1883, 2080, 2701
BcgI CGANNNNNNTGC 2 cut(s) 1625, 1659
BciVI GTATCC 4 cut(s) 804, 869, 1611, 2551
BclI TGATCA 2 cut(s) 465, 1533
BcnI CCSGG 1 cut(s) 1787
BcoDI GTCTC 8 cut(s) 512, 912, 1373, 1484, 1559, 2103, 2363, 2663
BfaI CTAG 7 cut(s) 384, 1095, 1619, 2328, 2711, 2745, 2807
BfmI CTRYAG 3 cut(s) 1733, 2419, 2482
BfoI RGCGCY 4 cut(s) 933, 1425, 1793, 2309
BfrI CTTAAG 1 cut(s) 182
BfuAI ACCTGC 2 cut(s) 617, 843
BfuI GTATCC 4 cut(s) 804, 869, 1611, 2551
BglII AGATCT 1 cut(s) 135
BlpI GCTNAGC 2 cut(s) 2640, 2715
Bme18I GGWCC 2 cut(s) 178, 2337
BmeT110I CYCGRG 1 cut(s) 47
BmgBI CACGTC 1 cut(s) 2779
BmgT120I GGNCC 7 cut(s) 143, 178, 244, 1098, 1333, 2337, 2463
BmiI GGNNCC 5 cut(s) 453, 578, 931, 1003, 2158
BmsI GCATC 9 cut(s) 480, 490, 1120, 1234, 1256, 1771, 1816, 2301, 2496
BmtI GCTAGC 1 cut(s) 2714
BoxI GACNNNNGTC 2 cut(s) 404, 2180
BpiI GAAGAC 3 cut(s) 27, 1415, 2176
BplI GAGNNNNNCTC 2 cut(s) 581, 613
BpmI CTGGAG 4 cut(s) 152, 1087, 1785, 2645
Bpu10I CCTNAGC 2 cut(s) 1284, 1704
Bpu1102I GCTNAGC 2 cut(s) 2640, 2715
BpuEI CTTGAG 7 cut(s) 176, 430, 512, 1295, 1529, 1778, 2108
BpuMI CCSGG 1 cut(s) 1787
Bsa29I ATCGAT 1 cut(s) 1242
BsaBI GATNNNNATC 1 cut(s) 470
BsaHI GRCGYC 4 cut(s) 24, 930, 1122, 2633
BsaI GGTCTC 5 cut(s) 912, 1373, 1559, 2363, 2663
BsaWI WCCGGW 5 cut(s) 551, 647, 998, 1568, 2668
BsaXI ACNNNNNCTCC 8 cut(s) 474, 504, 576, 606, 1353, 1383, 1758, 1788
Bsc4I CCNNNNNNNGG 9 cut(s) 64, 253, 264, 573, 1165, 1378, 1575, 1975, 2668
Bse118I RCCGGY 8 cut(s) 356, 551, 647, 998, 1424, 1568, 2465, 2543
Bse1I ACTGG 5 cut(s) 287, 1262, 1543, 1768, 2146
Bse3DI GCAATG 6 cut(s) 367, 639, 756, 864, 1641, 2633
Bse8I GATNNNNATC 1 cut(s) 470
BseAI TCCGGA 1 cut(s) 2668
BseCI ATCGAT 1 cut(s) 1242
BseJI GATNNNNATC 1 cut(s) 470
BseLI CCNNNNNNNGG 9 cut(s) 64, 253, 264, 573, 1165, 1378, 1575, 1975, 2668
BseMI GCAATG 6 cut(s) 367, 639, 756, 864, 1641, 2633
BseNI ACTGG 5 cut(s) 287, 1262, 1543, 1768, 2146
BsePI GCGCGC 1 cut(s) 2731
BseRI GAGGAG 2 cut(s) 173, 2091
BseSI GKGCMC 3 cut(s) 1282, 2238, 2778
BseX3I CGGCCG 1 cut(s) 108
BseXI GCAGC 9 cut(s) 91, 220, 352, 383, 529, 661, 1281, 1850, 2396
BseYI CCCAGC 2 cut(s) 493, 968
BsgI GTGCAG 1 cut(s) 2744
Bsh1236I CGCG 5 cut(s) 505, 1365, 1850, 2733, 2735
Bsh1285I CGRYCG 2 cut(s) 111, 1573
BshNI GGYRCC 3 cut(s) 451, 929, 1001
BshTI ACCGGT 4 cut(s) 551, 647, 998, 1568
BshVI ATCGAT 1 cut(s) 1242
BsiEI CGRYCG 2 cut(s) 111, 1573
BsiHKAI GWGCWC 6 cut(s) 1282, 1507, 1515, 1668, 1710, 2238
BsiHKCI CYCGRG 1 cut(s) 47
BslFI GGGAC 3 cut(s) 476, 544, 2074
BslI CCNNNNNNNGG 9 cut(s) 64, 253, 264, 573, 1165, 1378, 1575, 1975, 2668
BsmAI GTCTC 8 cut(s) 512, 912, 1373, 1484, 1559, 2103, 2363, 2663
BsmBI CGTCTC 2 cut(s) 512, 2103
BsmFI GGGAC 3 cut(s) 476, 544, 2074
Bso31I GGTCTC 5 cut(s) 912, 1373, 1559, 2363, 2663
BsoBI CYCGRG 1 cut(s) 47
Bsp1286I GDGCHC 7 cut(s) 1282, 1507, 1515, 1668, 1710, 2238, 2778
Bsp13I TCCGGA 1 cut(s) 2668
Bsp1720I GCTNAGC 2 cut(s) 2640, 2715
Bsp19I CCATGG 2 cut(s) 894, 1433
BspDI ATCGAT 1 cut(s) 1242
BspEI TCCGGA 1 cut(s) 2668
BspFNI CGCG 5 cut(s) 505, 1365, 1850, 2733, 2735
BspHI TCATGA 2 cut(s) 2067, 2172
BspLI GGNNCC 5 cut(s) 453, 578, 931, 1003, 2158
BspMAI CTGCAG 1 cut(s) 2423
BspMI ACCTGC 2 cut(s) 617, 843
BspOI GCTAGC 1 cut(s) 2714
BspPI GGATC 4 cut(s) 515, 775, 1460, 2486
BspT107I GGYRCC 3 cut(s) 451, 929, 1001
BspTI CTTAAG 1 cut(s) 182
BspTNI GGTCTC 5 cut(s) 912, 1373, 1559, 2363, 2663
BsrBI CCGCTC 1 cut(s) 1374
BsrDI GCAATG 6 cut(s) 367, 639, 756, 864, 1641, 2633
BsrFI RCCGGY 8 cut(s) 356, 551, 647, 998, 1424, 1568, 2465, 2543
BsrI ACTGG 5 cut(s) 287, 1262, 1543, 1768, 2146
BssAI RCCGGY 8 cut(s) 356, 551, 647, 998, 1424, 1568, 2465, 2543
BssHII GCGCGC 1 cut(s) 2731
BssNI GRCGYC 4 cut(s) 24, 930, 1122, 2633
BssT1I CCWWGG 6 cut(s) 894, 1161, 1413, 1433, 2079, 2152
Bst4CI ACNGT 7 cut(s) 880, 1027, 1399, 1484, 2179, 2657, 2839
Bst6I CTCTTC 1 cut(s) 954
BstACI GRCGYC 4 cut(s) 24, 930, 1122, 2633
BstAFI CTTAAG 1 cut(s) 182
BstAPI GCANNNNNTGC 1 cut(s) 1198
BstDSI CCRYGG 7 cut(s) 876, 894, 1433, 1818, 2178, 2683, 2692
BstEII GGTNACC 1 cut(s) 1541
BstFNI CGCG 5 cut(s) 505, 1365, 1850, 2733, 2735
BstH2I RGCGCY 4 cut(s) 933, 1425, 1793, 2309
BstMAI GTCTC 8 cut(s) 512, 912, 1373, 1484, 1559, 2103, 2363, 2663
BstMCI CGRYCG 2 cut(s) 111, 1573
BstNSI RCATGY 1 cut(s) 1600
BstPAI GACNNNNGTC 2 cut(s) 404, 2180
BstPI GGTNACC 1 cut(s) 1541
BstSFI CTRYAG 3 cut(s) 1733, 2419, 2482
BstSLI GKGCMC 3 cut(s) 1282, 2238, 2778
BstUI CGCG 5 cut(s) 505, 1365, 1850, 2733, 2735
BstV1I GCAGC 9 cut(s) 91, 220, 352, 383, 529, 661, 1281, 1850, 2396
BstV2I GAAGAC 3 cut(s) 27, 1415, 2176
BstX2I RGATCY 3 cut(s) 135, 780, 2478
BstXI CCANNNNNNTGG 3 cut(s) 282, 703, 763
BstYI RGATCY 3 cut(s) 135, 780, 2478
BstZI CGGCCG 1 cut(s) 108
Bsu15I ATCGAT 1 cut(s) 1242
BsuI GTATCC 4 cut(s) 804, 869, 1611, 2551
BsuTUI ATCGAT 1 cut(s) 1242
BtgI CCRYGG 7 cut(s) 876, 894, 1433, 1818, 2178, 2683, 2692
BtgZI GCGATG 1 cut(s) 2392
BtrI CACGTC 1 cut(s) 2779
BtsI GCAGTG 1 cut(s) 994
BtsIMutI CAGTG 7 cut(s) 994, 1182, 1489, 1536, 2139, 2793, 2835
BveI ACCTGC 2 cut(s) 617, 843
CaiI CAGNNNCTG 1 cut(s) 1403
CciI TCATGA 2 cut(s) 2067, 2172
Cfr10I RCCGGY 8 cut(s) 356, 551, 647, 998, 1424, 1568, 2465, 2543
Cfr13I GGNCC 7 cut(s) 143, 178, 244, 1098, 1333, 2337, 2463
ClaI ATCGAT 1 cut(s) 1242
CseI GACGC 5 cut(s) 494, 1130, 1839, 1844, 2622
Csp6I GTAC 2 cut(s) 1002, 2416
CspAI ACCGGT 4 cut(s) 551, 647, 998, 1568
CspCI CAANNNNNGTGG 2 cut(s) 2128, 2163
CviQI GTAC 2 cut(s) 1002, 2416
DinI GGCGCC 1 cut(s) 931
DraIII CACNNNGTG 1 cut(s) 685
EaeI YGGCCR 6 cut(s) 3, 108, 255, 284, 1152, 2397
EagI CGGCCG 1 cut(s) 108
Eam1104I CTCTTC 1 cut(s) 954
EarI CTCTTC 1 cut(s) 954
EciI GGCGGA 3 cut(s) 1487, 1817, 2720
Ecl136II GAGCTC 2 cut(s) 1513, 1666
EclXI CGGCCG 1 cut(s) 108
Eco130I CCWWGG 6 cut(s) 894, 1161, 1413, 1433, 2079, 2152
Eco147I AGGCCT 1 cut(s) 2552
Eco24I GRGCYC 2 cut(s) 1515, 1668
Eco31I GGTCTC 5 cut(s) 912, 1373, 1559, 2363, 2663
Eco32I GATATC 2 cut(s) 487, 2512
Eco47I GGWCC 2 cut(s) 178, 2337
Eco47III AGCGCT 1 cut(s) 2307
Eco52I CGGCCG 1 cut(s) 108
Eco53kI GAGCTC 2 cut(s) 1513, 1666
Eco57I CTGAAG 1 cut(s) 2819
Eco88I CYCGRG 1 cut(s) 47
Eco91I GGTNACC 1 cut(s) 1541
EcoICRI GAGCTC 2 cut(s) 1513, 1666
EcoO109I RGGNCCY 2 cut(s) 178, 1098
EcoO65I GGTNACC 1 cut(s) 1541
EcoRV GATATC 2 cut(s) 487, 2512
EcoT14I CCWWGG 6 cut(s) 894, 1161, 1413, 1433, 2079, 2152
EcoT38I GRGCYC 2 cut(s) 1515, 1668
EgeI GGCGCC 1 cut(s) 931
EheI GGCGCC 1 cut(s) 931
ErhI CCWWGG 6 cut(s) 894, 1161, 1413, 1433, 2079, 2152
Esp3I CGTCTC 2 cut(s) 512, 2103
FalI AAGNNNNNCTT 6 cut(s) 2000, 2032, 2117, 2149, 2567, 2599
FaqI GGGAC 3 cut(s) 476, 544, 2074
FauI CCCGC 1 cut(s) 1748
FauNDI CATATG 1 cut(s) 749
FbaI TGATCA 2 cut(s) 465, 1533
FblI GTMKAC 2 cut(s) 42, 1215
FriOI GRGCYC 2 cut(s) 1515, 1668
FspBI CTAG 7 cut(s) 384, 1095, 1619, 2328, 2711, 2745, 2807
GsaI CCCAGC 2 cut(s) 497, 972
GsuI CTGGAG 4 cut(s) 152, 1087, 1785, 2645
HaeII RGCGCY 4 cut(s) 933, 1425, 1793, 2309
HgaI GACGC 5 cut(s) 494, 1130, 1839, 1844, 2622
Hin1I GRCGYC 4 cut(s) 24, 930, 1122, 2633
HincII GTYRAC 4 cut(s) 43, 726, 1216, 1522
HindII GTYRAC 4 cut(s) 43, 726, 1216, 1522
HindIII AAGCTT 2 cut(s) 152, 1019
Hpy166II GTNNAC 8 cut(s) 43, 726, 1216, 1280, 1451, 1522, 2236, 2416
Hpy188I TCNGA 8 cut(s) 378, 668, 737, 849, 854, 1855, 2219, 2248
Hpy8I GTNNAC 8 cut(s) 43, 726, 1216, 1280, 1451, 1522, 2236, 2416
Hpy99I CGWCG 6 cut(s) 44, 299, 1031, 1217, 1855, 2783
HpyCH4III ACNGT 7 cut(s) 880, 1027, 1399, 1484, 2179, 2657, 2839
HpyCH4IV ACGT 7 cut(s) 24, 39, 978, 1062, 1524, 2765, 2778
HpySE526I ACGT 7 cut(s) 24, 39, 978, 1062, 1524, 2765, 2778
Hsp92I GRCGYC 4 cut(s) 24, 930, 1122, 2633
KasI GGCGCC 1 cut(s) 929
Kpn2I TCCGGA 1 cut(s) 2668
KpnI GGTACC 1 cut(s) 1005
KroI GCCGGC 2 cut(s) 356, 1424
KroNI GCCGGC 2 cut(s) 358, 1426
Ksp22I TGATCA 2 cut(s) 465, 1533
LmnI GCTCC 6 cut(s) 430, 758, 1845, 2331, 2698, 2707
Lsp1109I GCAGC 9 cut(s) 91, 220, 352, 383, 529, 661, 1281, 1850, 2396
LweI GCATC 9 cut(s) 480, 490, 1120, 1234, 1256, 1771, 1816, 2301, 2496
MaeI CTAG 7 cut(s) 384, 1095, 1619, 2328, 2711, 2745, 2807
MaeII ACGT 7 cut(s) 24, 39, 978, 1062, 1524, 2765, 2778
MaeIII GTNAC 6 cut(s) 568, 707, 889, 1541, 2317, 2491
MbiI CCGCTC 1 cut(s) 1374
MflI RGATCY 3 cut(s) 135, 780, 2478
MhlI GDGCHC 7 cut(s) 1282, 1507, 1515, 1668, 1710, 2238, 2778
MlsI TGGCCA 4 cut(s) 5, 257, 286, 2399
MluCI AATT 3 cut(s) 774, 798, 1041
MluNI TGGCCA 4 cut(s) 5, 257, 286, 2399
Mly113I GGCGCC 1 cut(s) 930
MlyI GAGTC 6 cut(s) 566, 1045, 1646, 1704, 2105, 2314
MmeI TCCRAC 2 cut(s) 1833, 2431
Mox20I TGGCCA 4 cut(s) 5, 257, 286, 2399
MroI TCCGGA 1 cut(s) 2668
MroNI GCCGGC 2 cut(s) 356, 1424
MscI TGGCCA 4 cut(s) 5, 257, 286, 2399
MseI TTAA 6 cut(s) 183, 1143, 1527, 2265, 2768, 2793
MslI CAYNNNNRTG 8 cut(s) 761, 869, 1187, 1482, 1713, 2177, 2315, 2411
Msp20I TGGCCA 4 cut(s) 5, 257, 286, 2399
MspA1I CMGCKG 2 cut(s) 82, 497
MspCI CTTAAG 1 cut(s) 182
MvnI CGCG 5 cut(s) 505, 1365, 1850, 2733, 2735
NaeI GCCGGC 2 cut(s) 358, 1426
NarI GGCGCC 1 cut(s) 930
NciI CCSGG 1 cut(s) 1787
NcoI CCATGG 2 cut(s) 894, 1433
NdeI CATATG 1 cut(s) 749
NgoMIV GCCGGC 2 cut(s) 356, 1424
NheI GCTAGC 1 cut(s) 2710
NlaIV GGNNCC 5 cut(s) 453, 578, 931, 1003, 2158
NmeAIII GCCGAG 3 cut(s) 136, 1560, 2249
NmuCI GTSAC 5 cut(s) 707, 889, 1541, 2317, 2491
NspI RCATGY 1 cut(s) 1600
OliI CACNNNNGTG 2 cut(s) 1187, 1482
PaeR7I CTCGAG 1 cut(s) 47
PagI TCATGA 2 cut(s) 2067, 2172
PauI GCGCGC 1 cut(s) 2731
PceI AGGCCT 1 cut(s) 2552
PciI ACATGT 1 cut(s) 1596
PcsI WCGNNNNNNNCGW 5 cut(s) 30, 39, 45, 1521, 2142
PdiI GCCGGC 2 cut(s) 358, 1426
PfeI GAWTC 6 cut(s) 54, 205, 1179, 1983, 2439, 2783
PflFI GACNNNGTC 2 cut(s) 293, 1859
PinAI ACCGGT 4 cut(s) 551, 647, 998, 1568
PleI GAGTC 6 cut(s) 565, 1044, 1645, 1703, 2105, 2314
PluTI GGCGCC 1 cut(s) 933
PpsI GAGTC 6 cut(s) 565, 1044, 1645, 1703, 2105, 2314
PpuMI RGGWCCY 1 cut(s) 178
PscI ACATGT 1 cut(s) 1596
PshAI GACNNNNGTC 2 cut(s) 404, 2180
Psp124BI GAGCTC 2 cut(s) 1515, 1668
Psp1406I AACGTT 1 cut(s) 1524
Psp5II RGGWCCY 1 cut(s) 178
PspEI GGTNACC 1 cut(s) 1541
PspFI CCCAGC 2 cut(s) 493, 968
PspN4I GGNNCC 5 cut(s) 453, 578, 931, 1003, 2158
PspPI GGNCC 7 cut(s) 143, 178, 244, 1098, 1333, 2337, 2463
PspPPI RGGWCCY 1 cut(s) 178
PspXI VCTCGAGB 1 cut(s) 47
PstI CTGCAG 1 cut(s) 2423
PstNI CAGNNNCTG 1 cut(s) 1403
PsuI RGATCY 3 cut(s) 135, 780, 2478
PsyI GACNNNGTC 2 cut(s) 293, 1859
PteI GCGCGC 1 cut(s) 2731
PvuII CAGCTG 2 cut(s) 82, 497
RsaI GTAC 2 cut(s) 1003, 2417
RsaNI GTAC 2 cut(s) 1002, 2416
RseI CAYNNNNRTG 8 cut(s) 761, 869, 1187, 1482, 1713, 2177, 2315, 2411
SacI GAGCTC 2 cut(s) 1515, 1668
SalI GTCGAC 2 cut(s) 41, 1214
SaqAI TTAA 6 cut(s) 183, 1143, 1527, 2265, 2768, 2793
Sau96I GGNCC 7 cut(s) 143, 178, 244, 1098, 1333, 2337, 2463
SchI GAGTC 6 cut(s) 566, 1045, 1646, 1704, 2105, 2314
SduI GDGCHC 7 cut(s) 1282, 1507, 1515, 1668, 1710, 2238, 2778
SfaNI GCATC 9 cut(s) 480, 490, 1120, 1234, 1256, 1771, 1816, 2301, 2496
SfcI CTRYAG 3 cut(s) 1733, 2419, 2482
SfoI GGCGCC 1 cut(s) 931
Sfr274I CTCGAG 1 cut(s) 47
SgrAI CRCCGGYG 1 cut(s) 551
SinI GGWCC 2 cut(s) 178, 2337
SlaI CTCGAG 1 cut(s) 47
SmiMI CAYNNNNRTG 8 cut(s) 761, 869, 1187, 1482, 1713, 2177, 2315, 2411
SmlI CTYRAG 9 cut(s) 47, 155, 182, 409, 527, 1310, 1508, 1793, 2123
SmoI CTYRAG 9 cut(s) 47, 155, 182, 409, 527, 1310, 1508, 1793, 2123
Sse9I AATT 3 cut(s) 774, 798, 1041
SseBI AGGCCT 1 cut(s) 2552
SspDI GGCGCC 1 cut(s) 929
SspI AATATT 1 cut(s) 2242
SspMI CTAG 7 cut(s) 384, 1095, 1619, 2328, 2711, 2745, 2807
SstI GAGCTC 2 cut(s) 1515, 1668
StuI AGGCCT 1 cut(s) 2552
StyI CCWWGG 6 cut(s) 894, 1161, 1413, 1433, 2079, 2152
TaaI ACNGT 7 cut(s) 880, 1027, 1399, 1484, 2179, 2657, 2839
TaiI ACGT 7 cut(s) 27, 42, 981, 1065, 1527, 2768, 2781
TasI AATT 3 cut(s) 774, 798, 1041
TatI WGTACW 1 cut(s) 2415
TauI GCSGC 2 cut(s) 130, 371
TfiI GAWTC 6 cut(s) 54, 205, 1179, 1983, 2439, 2783
Tru1I TTAA 6 cut(s) 183, 1143, 1527, 2265, 2768, 2793
Tru9I TTAA 6 cut(s) 183, 1143, 1527, 2265, 2768, 2793
TscAI CASTG 7 cut(s) 994, 1189, 1489, 1543, 2146, 2793, 2842
TseFI GTSAC 5 cut(s) 707, 889, 1541, 2317, 2491
TseI GCWGC 9 cut(s) 79, 233, 365, 371, 542, 674, 1269, 1838, 2384
Tsp45I GTSAC 5 cut(s) 707, 889, 1541, 2317, 2491
TspDTI ATGAA 5 cut(s) 328, 1946, 1975, 2017, 2512
TspGWI ACGGA 4 cut(s) 877, 1593, 1958, 2619
TspRI CASTG 7 cut(s) 994, 1189, 1489, 1543, 2146, 2793, 2842
Tth111I GACNNNGTC 2 cut(s) 293, 1859
Vha464I CTTAAG 1 cut(s) 182
VneI GTGCAC 2 cut(s) 1278, 2234
VpaK11BI GGWCC 2 cut(s) 178, 2337
XceI RCATGY 1 cut(s) 1600
XcmI CCANNNNNNNNNTGG 2 cut(s) 961, 1553
XhoI CTCGAG 1 cut(s) 47
XmiI GTMKAC 2 cut(s) 42, 1215
XspI CTAG 7 cut(s) 384, 1095, 1619, 2328, 2711, 2745, 2807
ZraI GACGTC 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.