FvH4_1g03200

SPX domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012998
Physical Location & Seq
Forward (+)
1794333 .. 1796969
2637 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g03200.t2

Sequence Viewer

Length: 843 bp
ATGAAGTTCGGCAAGAGTTTGAGCAGCCAGATCGACGAGACCCTGCCGGAATGGCGCGACAAGTTCCTCTCCTACAAGGAGCTCAAGAAGCGCCTCAAGCTCATCGAGCCCAAGGACGCCTCCACCCGCCCCGCCAAGCGCCCCAGGCTCGACGGCGACGCTCCTCCGGACGACATGTCGTCCGAGGAGATCAATTTCATTCAGCTCCTCGAGGACGAGCTCGAGAAGTTCAATTCCTTCTTCGTCGAGAAGGAAGAAGAGTATATCATTAGACTGAAGGAAATACAAGACAGTGTGGCGAAAGCGAAGCATTCGACTGAAGAGGTTATGAAGATACGCAAGGAGATTGTGGATTTCCATGGAGAGATGGTTTTGTTGGAGAATTACAGTGCCCTGAACTATACAGGACTGGTAAAGATACTCAAGAAGTACGACAAAAGAACAGGTGCTCTTATGCGCTTGCCCTTTATTCAGAAGGTCCTGCAACAACCTTTCTTCACTACTGACTTGCTCTACAAGCTTGTGAAGGAGTGCGAGTGCACACTTGATCAGCTGTTTGCAATGTGTGAACCGCCAGCTTCAAATGAAGCATCTGATGATGGGGATGATGGTCCCTCGACTTCCAAGAGTGATGGACTTAGCAGAGTGAACAAGGAACTTGCAGAGATAGAGTACATCGAGAGCTTGTACATGAAAAGCACTATATCAGCGTTGCGAGCTTTGAAAGAAATTAGAAGTGGAAGTTCCACTGTGAGTGCATTTTCATTGCCACCACTGCAATTAAGTGGTTTAGATGAAGATGCCTGGAAAAAGGTTCCTGTTCTGGAACAGCTTGCTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPX

Protein Analysis

281

Amino Acids

31.99

Weight (kDa)

5.17

Isoelectric Point (pI)

54.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPX PF03105 46 - 99 1.8e-11 SPX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012998)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20150
fragaria_vesca FvH4_1g03200 FvH4_1g03200
malus_domestica MD02G1031100.v1.1 MD15G1172700.v1.1
prunus_persica Prupe.7G243500_v2.0.a1
pyrus_communis pycom02g02600 pycom15g15440
rosa_chinensis RchiOBHm_Chr2g0088351
rosa_laevigata RLG00000015944
rosa_multiflora Rmu_sc0004235.1_g000027
rosa_roxburghii Rroxscaffold_2G00152740
rosa_rugosa Rorug01G0483000
rosa_samantha Rh2AG038000 Rh2BG037300 Rh2CG038400 Rh2DG037900
rosa_wichuraiana Rw2G003010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 209
AccII CGCG 1 cut(s) 57
AccIII TCCGGA 1 cut(s) 166
AciI CCGC 3 cut(s) 127, 132, 572
AcuI CTGAAG 2 cut(s) 296, 339
AcyI GRCGYC 1 cut(s) 117
AfaI GTAC 3 cut(s) 431, 674, 689
AflIII ACRYGT 1 cut(s) 174
AgsI TTSAA 3 cut(s) 232, 582, 724
AhdI GACNNNNNGTC 2 cut(s) 175, 178
AjnI CCWGG 2 cut(s) 143, 803
Alw21I GWGCWC 4 cut(s) 84, 222, 451, 542
Alw26I GTCTC 1 cut(s) 32
Alw44I GTGCAC 1 cut(s) 538
Ama87I CYCGRG 2 cut(s) 209, 221
Aor13HI TCCGGA 1 cut(s) 166
ApaLI GTGCAC 1 cut(s) 538
ApeKI GCWGC 1 cut(s) 24
AspLEI GCGC 4 cut(s) 57, 93, 141, 459
AspS9I GGNCC 2 cut(s) 478, 611
AvaI CYCGRG 2 cut(s) 209, 221
AvaII GGWCC 2 cut(s) 478, 611
BaeGI GKGCMC 2 cut(s) 394, 542
BanII GRGCYC 3 cut(s) 84, 111, 222
Bbv12I GWGCWC 4 cut(s) 84, 222, 451, 542
BbvI GCAGC 1 cut(s) 36
BccI CCATC 4 cut(s) 361, 593, 602, 626
BceAI ACGGC 1 cut(s) 169
BcgI CGANNNNNNTGC 2 cut(s) 13, 47
BciT130I CCWGG 2 cut(s) 145, 805
BclI TGATCA 1 cut(s) 547
BcoDI GTCTC 1 cut(s) 32
BfoI RGCGCY 2 cut(s) 94, 142
BglI GCCNNNNNGGC 1 cut(s) 52
BisI GCNGC 1 cut(s) 25
BlsI GCNGC 1 cut(s) 26
Bme1390I CCNGG 2 cut(s) 145, 805
Bme18I GGWCC 2 cut(s) 478, 611
BmeRI GACNNNNNGTC 2 cut(s) 175, 178
BmeT110I CYCGRG 2 cut(s) 209, 221
BmgT120I GGNCC 2 cut(s) 478, 611
BmiI GGNNCC 2 cut(s) 613, 816
BmrFI CCNGG 2 cut(s) 145, 805
BmsI GCATC 2 cut(s) 599, 790
BpuEI CTTGAG 3 cut(s) 68, 80, 407
BsaHI GRCGYC 1 cut(s) 117
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 4 cut(s) 111, 143, 183, 358
BsaWI WCCGGW 1 cut(s) 166
BsaXI ACNNNNNCTCC 4 cut(s) 354, 384, 521, 551
Bse1I ACTGG 1 cut(s) 414
Bse3DI GCAATG 2 cut(s) 567, 764
BseAI TCCGGA 1 cut(s) 166
BseBI CCWGG 2 cut(s) 145, 805
BseDI CCNNGG 4 cut(s) 111, 143, 183, 358
BseGI GGATG 1 cut(s) 610
BseMI GCAATG 2 cut(s) 567, 764
BseNI ACTGG 1 cut(s) 414
BseRI GAGGAG 3 cut(s) 153, 197, 200
BseSI GKGCMC 2 cut(s) 394, 542
BseXI GCAGC 1 cut(s) 36
Bsh1236I CGCG 1 cut(s) 57
BsiHKAI GWGCWC 4 cut(s) 84, 222, 451, 542
BsiHKCI CYCGRG 2 cut(s) 209, 221
BsiSI CCGG 2 cut(s) 47, 167
BslFI GGGAC 1 cut(s) 597
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 1 cut(s) 597
BsmI GAATGC 1 cut(s) 310
Bso31I GGTCTC 1 cut(s) 32
BsoBI CYCGRG 2 cut(s) 209, 221
Bsp1286I GDGCHC 6 cut(s) 84, 111, 222, 394, 451, 542
Bsp13I TCCGGA 1 cut(s) 166
Bsp1407I TGTACA 1 cut(s) 687
Bsp143I GATC 3 cut(s) 30, 189, 547
Bsp19I CCATGG 1 cut(s) 358
BspACI CCGC 3 cut(s) 127, 132, 572
BspEI TCCGGA 1 cut(s) 166
BspFNI CGCG 1 cut(s) 57
BspLI GGNNCC 2 cut(s) 613, 816
BspTNI GGTCTC 1 cut(s) 32
BsrDI GCAATG 2 cut(s) 567, 764
BsrGI TGTACA 1 cut(s) 687
BsrI ACTGG 1 cut(s) 414
BssECI CCNNGG 4 cut(s) 111, 143, 183, 358
BssMI GATC 3 cut(s) 30, 189, 547
BssNI GRCGYC 1 cut(s) 117
BssT1I CCWWGG 2 cut(s) 111, 358
Bst2UI CCWGG 2 cut(s) 145, 805
Bst4CI ACNGT 3 cut(s) 293, 389, 751
Bst6I CTCTTC 2 cut(s) 252, 315
BstACI GRCGYC 1 cut(s) 117
BstAUI TGTACA 1 cut(s) 687
BstC8I GCNNGC 4 cut(s) 461, 576, 717, 834
BstDEI CTNAG 2 cut(s) 638, 837
BstDSI CCRYGG 1 cut(s) 358
BstF5I GGATG 1 cut(s) 610
BstFNI CGCG 1 cut(s) 57
BstH2I RGCGCY 2 cut(s) 94, 142
BstHHI GCGC 4 cut(s) 57, 93, 141, 459
BstKTI GATC 3 cut(s) 33, 192, 550
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 3 cut(s) 30, 189, 547
BstMWI GCNNNNNNNGC 8 cut(s) 52, 88, 97, 106, 145, 517, 716, 775
BstNI CCWGG 2 cut(s) 145, 805
BstNSI RCATGY 1 cut(s) 178
BstSCI CCNGG 2 cut(s) 143, 803
BstSLI GKGCMC 2 cut(s) 394, 542
BstUI CGCG 1 cut(s) 57
BstV1I GCAGC 1 cut(s) 36
BtgI CCRYGG 1 cut(s) 358
BtsCI GGATG 1 cut(s) 610
BtsI GCAGTG 1 cut(s) 773
BtsIMutI CAGTG 4 cut(s) 298, 394, 747, 773
Cac8I GCNNGC 4 cut(s) 461, 576, 717, 834
CfoI GCGC 4 cut(s) 57, 93, 141, 459
Cfr13I GGNCC 2 cut(s) 478, 611
CseI GACGC 2 cut(s) 125, 167
Csp6I GTAC 3 cut(s) 430, 673, 688
CviAII CATG 3 cut(s) 175, 359, 691
CviQI GTAC 3 cut(s) 430, 673, 688
DdeI CTNAG 2 cut(s) 638, 837
DpnI GATC 3 cut(s) 32, 191, 549
DpnII GATC 3 cut(s) 30, 189, 547
DriI GACNNNNNGTC 2 cut(s) 175, 178
Eam1104I CTCTTC 2 cut(s) 252, 315
Eam1105I GACNNNNNGTC 2 cut(s) 175, 178
EarI CTCTTC 2 cut(s) 252, 315
Ecl136II GAGCTC 2 cut(s) 82, 220
Eco130I CCWWGG 2 cut(s) 111, 358
Eco24I GRGCYC 3 cut(s) 84, 111, 222
Eco31I GGTCTC 1 cut(s) 32
Eco47I GGWCC 2 cut(s) 478, 611
Eco53kI GAGCTC 2 cut(s) 82, 220
Eco57I CTGAAG 2 cut(s) 296, 339
Eco88I CYCGRG 2 cut(s) 209, 221
EcoICRI GAGCTC 2 cut(s) 82, 220
EcoO109I RGGNCCY 1 cut(s) 478
EcoRII CCWGG 2 cut(s) 143, 803
EcoT14I CCWWGG 2 cut(s) 111, 358
EcoT38I GRGCYC 3 cut(s) 84, 111, 222
ErhI CCWWGG 2 cut(s) 111, 358
FaeI CATG 3 cut(s) 178, 362, 694
FaiI YATR 8 cut(s) 176, 264, 329, 360, 402, 455, 692, 704
FaqI GGGAC 1 cut(s) 597
FatI CATG 3 cut(s) 174, 358, 690
FauI CCCGC 2 cut(s) 134, 139
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 1 cut(s) 25
FokI GGATG 1 cut(s) 617
FriOI GRGCYC 3 cut(s) 84, 111, 222
Fsp4HI GCNGC 1 cut(s) 25
GlaI GCGC 4 cut(s) 56, 92, 140, 458
GluI GCNGC 1 cut(s) 25
HaeII RGCGCY 2 cut(s) 94, 142
HapII CCGG 2 cut(s) 47, 167
HgaI GACGC 2 cut(s) 125, 167
HhaI GCGC 4 cut(s) 57, 93, 141, 459
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 3 cut(s) 178, 362, 694
Hin6I GCGC 4 cut(s) 55, 91, 139, 457
HinP1I GCGC 4 cut(s) 55, 91, 139, 457
HindIII AAGCTT 1 cut(s) 518
HpaII CCGG 2 cut(s) 47, 167
Hpy166II GTNNAC 3 cut(s) 540, 569, 649
Hpy188I TCNGA 3 cut(s) 184, 474, 595
Hpy188III TCNNGA 7 cut(s) 85, 167, 223, 247, 424, 679, 824
Hpy8I GTNNAC 3 cut(s) 540, 569, 649
Hpy99I CGWCG 4 cut(s) 38, 155, 161, 248
HpyAV CCTTC 5 cut(s) 244, 247, 271, 469, 520
HpyCH4III ACNGT 3 cut(s) 293, 389, 751
HpyCH4V TGCA 6 cut(s) 484, 540, 560, 662, 758, 778
HpyF10VI GCNNNNNNNGC 8 cut(s) 52, 88, 97, 106, 145, 517, 716, 775
HpyF3I CTNAG 2 cut(s) 638, 837
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 3 cut(s) 178, 362, 694
HspAI GCGC 4 cut(s) 55, 91, 139, 457
Kpn2I TCCGGA 1 cut(s) 166
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 3 cut(s) 30, 189, 547
LmnI GCTCC 3 cut(s) 79, 166, 210
Lsp1109I GCAGC 1 cut(s) 36
LweI GCATC 2 cut(s) 599, 790
MalI GATC 3 cut(s) 32, 191, 549
MboI GATC 3 cut(s) 30, 189, 547
MboII GAAGA 7 cut(s) 232, 266, 269, 332, 343, 487, 809
MhlI GDGCHC 6 cut(s) 84, 111, 222, 394, 451, 542
MluCI AATT 5 cut(s) 193, 232, 382, 729, 779
MmeI TCCRAC 1 cut(s) 357
MnlI CCTC 9 cut(s) 77, 104, 130, 174, 178, 205, 218, 316, 625
MroI TCCGGA 1 cut(s) 166
MseI TTAA 1 cut(s) 782
MspA1I CMGCKG 1 cut(s) 553
MspI CCGG 2 cut(s) 47, 167
MspR9I CCNGG 2 cut(s) 145, 805
Mva1269I GAATGC 1 cut(s) 310
MvaI CCWGG 2 cut(s) 145, 805
MvnI CGCG 1 cut(s) 57
MwoI GCNNNNNNNGC 8 cut(s) 52, 88, 97, 106, 145, 517, 716, 775
NcoI CCATGG 1 cut(s) 358
NdeII GATC 3 cut(s) 30, 189, 547
NlaIII CATG 3 cut(s) 178, 362, 694
NlaIV GGNNCC 2 cut(s) 613, 816
NspI RCATGY 1 cut(s) 178
PaeR7I CTCGAG 2 cut(s) 209, 221
PciI ACATGT 1 cut(s) 174
PctI GAATGC 1 cut(s) 310
PkrI GCNGC 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 478
PscI ACATGT 1 cut(s) 174
Psp124BI GAGCTC 2 cut(s) 84, 222
Psp5II RGGWCCY 1 cut(s) 478
Psp6I CCWGG 2 cut(s) 143, 803
PspGI CCWGG 2 cut(s) 143, 803
PspN4I GGNNCC 2 cut(s) 613, 816
PspPI GGNCC 2 cut(s) 478, 611
PspPPI RGGWCCY 1 cut(s) 478
PspXI VCTCGAGB 1 cut(s) 209
PvuII CAGCTG 1 cut(s) 553
RsaI GTAC 3 cut(s) 431, 674, 689
RsaNI GTAC 3 cut(s) 430, 673, 688
SacI GAGCTC 2 cut(s) 84, 222
SaqAI TTAA 1 cut(s) 782
SatI GCNGC 1 cut(s) 25
Sau3AI GATC 3 cut(s) 30, 189, 547
Sau96I GGNCC 2 cut(s) 478, 611
ScrFI CCNGG 2 cut(s) 145, 805
SduI GDGCHC 6 cut(s) 84, 111, 222, 394, 451, 542
SfaNI GCATC 2 cut(s) 599, 790
Sfr274I CTCGAG 2 cut(s) 209, 221
SinI GGWCC 2 cut(s) 478, 611
SlaI CTCGAG 2 cut(s) 209, 221
SmlI CTYRAG 5 cut(s) 83, 95, 209, 221, 422
SmoI CTYRAG 5 cut(s) 83, 95, 209, 221, 422
Sse9I AATT 5 cut(s) 193, 232, 382, 729, 779
SsiI CCGC 3 cut(s) 127, 132, 572
SstI GAGCTC 2 cut(s) 84, 222
StyD4I CCNGG 2 cut(s) 143, 803
StyI CCWWGG 2 cut(s) 111, 358
TaaI ACNGT 3 cut(s) 293, 389, 751
TaqI TCGA 9 cut(s) 33, 105, 150, 210, 222, 246, 314, 617, 678
TasI AATT 5 cut(s) 193, 232, 382, 729, 779
TatI WGTACW 2 cut(s) 672, 687
Tru1I TTAA 1 cut(s) 782
Tru9I TTAA 1 cut(s) 782
TscAI CASTG 4 cut(s) 298, 394, 754, 780
TseI GCWGC 1 cut(s) 24
TspDTI ATGAA 7 cut(s) 17, 187, 344, 600, 707, 753, 810
TspRI CASTG 4 cut(s) 298, 394, 754, 780
VneI GTGCAC 1 cut(s) 538
VpaK11BI GGWCC 2 cut(s) 478, 611
XceI RCATGY 1 cut(s) 178
XhoI CTCGAG 2 cut(s) 209, 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.