Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0012998

Physical Location & Seq

Genomic Coordinates Forward (+)

2446537 .. 2449320

Gene Structure 2784 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1031100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 870 bp

ATGAAGTTCGGTAAGAGCCTGAGCAACCAGATGGAAGAGACCCTGCCGGAATGGCGGGACAAGTTCTTGTCCTACAAAGAGCTCAAGAAGCGGTTGAAGCTCATCGAACCCAAAGGTGGCGAACGCCCCGCCAAGCGCCCCAGAATTGACGCCTCCGCCGCCGATTGTAACGACGGCGGCAACAAGGAGGGCATTTCTGCGGCGGAGATCAGTTTCATTACGTTGTTGGAGGACGAGCTCGAAAAATTTAACTCCTTCTTCCTCGAGAAGGAGGAAGAATACATTATTAGATTGAAGGAGATACAAGACAGGGTGGCGAAAGCAAAGCCTTTCCCGGACGAGATTATGAAGATCCGCAAGGAGATTGTGGACTTCCACGGAGAGATGGTTCTGTTGGAGAATTACAGTGCTCTCAACTATACAGGACTCGTGAAGATACTCAAGAAGTACGATAAAAGAACTGGTGCTCTTATGCGCTTGCCCTTTATCCAGAAGGTCTTGCAACAGCCCTTCTTCACCATGGACTTGCTCTACAAGCTCATCAAGGAGTGCGAGATAACGCTTGACTATCTCTTTTCCATGAACGACCATCCCGTCTCAACTGAAGGATCTGATGGGGACGACAGGCGCAATCCTTCAACTTCTGCCCCTATCGAGAGTCATGGTGTTCATAGAGTAACCAAGGAACTTGCAGAGATTGAGTCTATGGAGAGCTTGTACATGAAAAGTATAATATCAGCGCTACGCGCGTTGAAGGAAATCAGGAGCGGCAGCTCAACCGTTAGCGTCTTCTCGTTGCCGCCGCTGCAGATGACCGGATTAGAAGATGCATGGAAAAAAGTCCCTGTGCTGGAACAACTTGCAAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0006950 GO:0007154 GO:0008150 GO:0009266 GO:0009267 GO:0009409 GO:0009605 GO:0009628 GO:0009987 GO:0009991 GO:0010646 GO:0010647 GO:0012505 GO:0016036 GO:0031667 GO:0031668 GO:0031669 GO:0032101 GO:0032103 GO:0032104 GO:0032106 GO:0032107 GO:0032109 GO:0033554 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070417 GO:0071496 GO:0080040 GO:0080134 GO:0080135

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SPX

Protein Analysis

290

Amino Acids

33.11

Weight (kDa)

6.2

Isoelectric Point (pI)

45.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPX	PF03105	1 - 37	7.6e-10	SPX domain
SPX	PF03105	117 - 166	1.3e-10	SPX domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012998)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20150
fragaria_vesca	FvH4_1g03200 FvH4_1g03200
malus_domestica	MD02G1031100.v1.1 MD15G1172700.v1.1
prunus_persica	Prupe.7G243500_v2.0.a1
pyrus_communis	pycom02g02600 pycom15g15440
rosa_chinensis	RchiOBHm_Chr2g0088351
rosa_laevigata	RLG00000015944
rosa_multiflora	Rmu_sc0004235.1_g000027
rosa_roxburghii	Rroxscaffold_2G00152740
rosa_rugosa	Rorug01G0483000
rosa_samantha	Rh2AG038000 Rh2BG037300 Rh2CG038400 Rh2DG037900
rosa_wichuraiana	Rw2G003010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	593
AccBSI	CCGCTC	1 cut(s)	768
AccII	CGCG	2 cut(s)	747, 749
AclWI	GGATC	2 cut(s)	346, 616
AcsI	RAATTY	1 cut(s)	245
AcuI	CTGAAG	1 cut(s)	624
AcyI	GRCGYC	1 cut(s)	150
AfaI	GTAC	2 cut(s)	449, 719
AfeI	AGCGCT	1 cut(s)	741
AfiI	CCNNNNNNNGG	3 cut(s)	116, 268, 850
AgsI	TTSAA	4 cut(s)	97, 295, 639, 754
AloI	GAACNNNNNNTCC	2 cut(s)	372, 404
AluBI	AGCT	6 cut(s)	82, 100, 238, 538, 714, 774
AluI	AGCT	6 cut(s)	82, 100, 238, 538, 714, 774
Alw21I	GWGCWC	4 cut(s)	84, 240, 412, 469
Alw26I	GTCTC	2 cut(s)	32, 601
AlwI	GGATC	2 cut(s)	346, 616
Ama87I	CYCGRG	1 cut(s)	263
Aor51HI	AGCGCT	1 cut(s)	741
ApeKI	GCWGC	2 cut(s)	771, 805
ApoI	RAATTY	1 cut(s)	245
AspLEI	GCGC	5 cut(s)	138, 477, 630, 742, 749
AsuC2I	CCSGG	1 cut(s)	335
AsuHPI	GGTGA	1 cut(s)	508
AvaI	CYCGRG	1 cut(s)	263
BanII	GRGCYC	2 cut(s)	84, 240
BauI	CACGAG	1 cut(s)	428
BbsI	GAAGAC	1 cut(s)	781
Bbv12I	GWGCWC	4 cut(s)	84, 240, 412, 469
BbvI	GCAGC	2 cut(s)	783, 792
BccI	CCATC	4 cut(s)	25, 379, 597, 608
BceAI	ACGGC	1 cut(s)	190
BcnI	CCSGG	1 cut(s)	335
BcoDI	GTCTC	2 cut(s)	32, 601
BfmI	CTRYAG	1 cut(s)	806
BfoI	RGCGCY	2 cut(s)	139, 743
BglI	GCCNNNNNGGC	1 cut(s)	52
BisI	GCNGC	8 cut(s)	159, 178, 201, 769, 772, 800, 803, 806
BlsI	GCNGC	8 cut(s)	160, 179, 202, 770, 773, 801, 804, 807
Bme1390I	CCNGG	1 cut(s)	335
BmeT110I	CYCGRG	1 cut(s)	263
BmrFI	CCNGG	1 cut(s)	335
BmsI	GCATC	1 cut(s)	817
BpiI	GAAGAC	1 cut(s)	781
Bpu10I	CCTNAGC	1 cut(s)	20
BpuEI	CTTGAG	2 cut(s)	68, 425
BpuMI	CCSGG	1 cut(s)	335
BsaHI	GRCGYC	1 cut(s)	150
BsaI	GGTCTC	1 cut(s)	32
BsaJI	CCNNGG	3 cut(s)	376, 519, 681
BsaWI	WCCGGW	1 cut(s)	815
BsaXI	ACNNNNNCTCC	4 cut(s)	372, 402, 701, 731
Bsc4I	CCNNNNNNNGG	3 cut(s)	116, 268, 850
Bse1I	ACTGG	1 cut(s)	466
BseDI	CCNNGG	3 cut(s)	376, 519, 681
BseGI	GGATG	1 cut(s)	589
BseLI	CCNNNNNNNGG	3 cut(s)	116, 268, 850
BseMII	CTCAG	1 cut(s)	11
BseNI	ACTGG	1 cut(s)	466
BseXI	GCAGC	2 cut(s)	783, 792
Bsh1236I	CGCG	2 cut(s)	747, 749
BsiHKAI	GWGCWC	4 cut(s)	84, 240, 412, 469
BsiHKCI	CYCGRG	1 cut(s)	263
BsiSI	CCGG	3 cut(s)	47, 335, 816
BslFI	GGGAC	3 cut(s)	71, 632, 827
BslI	CCNNNNNNNGG	3 cut(s)	116, 268, 850
BsmAI	GTCTC	2 cut(s)	32, 601
BsmBI	CGTCTC	1 cut(s)	601
BsmFI	GGGAC	3 cut(s)	71, 632, 827
Bso31I	GGTCTC	1 cut(s)	32
BsoBI	CYCGRG	1 cut(s)	263
Bsp1286I	GDGCHC	4 cut(s)	84, 240, 412, 469
Bsp1407I	TGTACA	1 cut(s)	717
Bsp143I	GATC	3 cut(s)	207, 351, 608
Bsp19I	CCATGG	1 cut(s)	519
BspCNI	CTCAG	1 cut(s)	12
BspFNI	CGCG	2 cut(s)	747, 749
BspMAI	CTGCAG	1 cut(s)	810
BspPI	GGATC	2 cut(s)	346, 616
BspTNI	GGTCTC	1 cut(s)	32
BsrBI	CCGCTC	1 cut(s)	768
BsrGI	TGTACA	1 cut(s)	717
BsrI	ACTGG	1 cut(s)	466
BssECI	CCNNGG	3 cut(s)	376, 519, 681
BssMI	GATC	3 cut(s)	207, 351, 608
BssNI	GRCGYC	1 cut(s)	150
BssSI	CACGAG	1 cut(s)	428
BssT1I	CCWWGG	2 cut(s)	519, 681
Bst2BI	CACGAG	1 cut(s)	428
Bst4CI	ACNGT	2 cut(s)	407, 781
Bst6I	CTCTTC	1 cut(s)	30
BstACI	GRCGYC	1 cut(s)	150
BstAUI	TGTACA	1 cut(s)	717
BstC8I	GCNNGC	1 cut(s)	479
BstDEI	CTNAG	1 cut(s)	20
BstDSI	CCRYGG	2 cut(s)	376, 519
BstENI	CCTNNNNNAGG	1 cut(s)	266
BstF5I	GGATG	1 cut(s)	589
BstFNI	CGCG	2 cut(s)	747, 749
BstH2I	RGCGCY	2 cut(s)	139, 743
BstHHI	GCGC	5 cut(s)	138, 477, 630, 742, 749
BstKTI	GATC	3 cut(s)	210, 354, 611
BstMAI	GTCTC	2 cut(s)	32, 601
BstMBI	GATC	3 cut(s)	207, 351, 608
BstMWI	GCNNNNNNNGC	7 cut(s)	52, 88, 97, 158, 535, 746, 805
BstSCI	CCNGG	1 cut(s)	333
BstSFI	CTRYAG	1 cut(s)	806
BstUI	CGCG	2 cut(s)	747, 749
BstV1I	GCAGC	2 cut(s)	783, 792
BstV2I	GAAGAC	1 cut(s)	781
BstX2I	RGATCY	2 cut(s)	351, 608
BstYI	RGATCY	2 cut(s)	351, 608
BtgI	CCRYGG	2 cut(s)	376, 519
BtsCI	GGATG	1 cut(s)	589
BtsIMutI	CAGTG	1 cut(s)	412
Cac8I	GCNNGC	1 cut(s)	479
CfoI	GCGC	5 cut(s)	138, 477, 630, 742, 749
CseI	GACGC	2 cut(s)	158, 775
Csp6I	GTAC	2 cut(s)	448, 718
CviAII	CATG	5 cut(s)	520, 580, 662, 721, 831
CviJI	RGCY	9 cut(s)	18, 82, 100, 238, 328, 508, 538, 714, 774
CviKI_1	RGCY	9 cut(s)	18, 82, 100, 238, 328, 508, 538, 714, 774
CviQI	GTAC	2 cut(s)	448, 718
DdeI	CTNAG	1 cut(s)	20
DpnI	GATC	3 cut(s)	209, 353, 610
DpnII	GATC	3 cut(s)	207, 351, 608
DrdI	GACNNNNNNGTC	1 cut(s)	593
DseDI	GACNNNNNNGTC	1 cut(s)	593
Eam1104I	CTCTTC	1 cut(s)	30
EarI	CTCTTC	1 cut(s)	30
EciI	GGCGGA	2 cut(s)	145, 218
Ecl136II	GAGCTC	2 cut(s)	82, 238
Eco130I	CCWWGG	2 cut(s)	519, 681
Eco24I	GRGCYC	2 cut(s)	84, 240
Eco31I	GGTCTC	1 cut(s)	32
Eco47III	AGCGCT	1 cut(s)	741
Eco53kI	GAGCTC	2 cut(s)	82, 238
Eco57I	CTGAAG	1 cut(s)	624
Eco88I	CYCGRG	1 cut(s)	263
EcoICRI	GAGCTC	2 cut(s)	82, 238
EcoNI	CCTNNNNNAGG	1 cut(s)	266
EcoT14I	CCWWGG	2 cut(s)	519, 681
EcoT22I	ATGCAT	1 cut(s)	832
EcoT38I	GRGCYC	2 cut(s)	84, 240
ErhI	CCWWGG	2 cut(s)	519, 681
Esp3I	CGTCTC	1 cut(s)	601
FaeI	CATG	5 cut(s)	523, 583, 665, 724, 834
FaqI	GGGAC	3 cut(s)	71, 632, 827
FatI	CATG	5 cut(s)	519, 579, 661, 720, 830
FauI	CCCGC	2 cut(s)	48, 136
Fnu4HI	GCNGC	8 cut(s)	159, 178, 201, 769, 772, 800, 803, 806
FokI	GGATG	1 cut(s)	576
FriOI	GRGCYC	2 cut(s)	84, 240
Fsp4HI	GCNGC	8 cut(s)	159, 178, 201, 769, 772, 800, 803, 806
GlaI	GCGC	5 cut(s)	137, 476, 629, 741, 748
GluI	GCNGC	8 cut(s)	159, 178, 201, 769, 772, 800, 803, 806
HaeII	RGCGCY	2 cut(s)	139, 743
HapII	CCGG	3 cut(s)	47, 335, 816
HgaI	GACGC	2 cut(s)	158, 775
HhaI	GCGC	5 cut(s)	138, 477, 630, 742, 749
Hin1I	GRCGYC	1 cut(s)	150
Hin1II	CATG	5 cut(s)	523, 583, 665, 724, 834
Hin6I	GCGC	5 cut(s)	136, 475, 628, 740, 747
HinP1I	GCGC	5 cut(s)	136, 475, 628, 740, 747
HinfI	GANTC	3 cut(s)	426, 658, 701
HpaII	CCGG	3 cut(s)	47, 335, 816
HphI	GGTGA	1 cut(s)	508
Hpy166II	GTNNAC	1 cut(s)	370
Hpy188I	TCNGA	1 cut(s)	613
Hpy188III	TCNNGA	7 cut(s)	85, 265, 430, 442, 490, 655, 763
Hpy8I	GTNNAC	1 cut(s)	370
Hpy99I	CGWCG	1 cut(s)	176
HpyAV	CCTTC	8 cut(s)	262, 265, 289, 487, 520, 599, 645, 748
HpyCH4III	ACNGT	2 cut(s)	407, 781
HpyCH4IV	ACGT	1 cut(s)	221
HpyCH4V	TGCA	5 cut(s)	502, 692, 808, 830, 863
HpyF10VI	GCNNNNNNNGC	7 cut(s)	52, 88, 97, 158, 535, 746, 805
HpyF3I	CTNAG	1 cut(s)	20
HpySE526I	ACGT	1 cut(s)	221
Hsp92I	GRCGYC	1 cut(s)	150
Hsp92II	CATG	5 cut(s)	523, 583, 665, 724, 834
HspAI	GCGC	5 cut(s)	136, 475, 628, 740, 747
Kzo9I	GATC	3 cut(s)	207, 351, 608
LmnI	GCTCC	1 cut(s)	765
Lsp1109I	GCAGC	2 cut(s)	783, 792
LweI	GCATC	1 cut(s)	817
MaeII	ACGT	1 cut(s)	221
MaeIII	GTNAC	2 cut(s)	167, 676
MalI	GATC	3 cut(s)	209, 353, 610
MbiI	CCGCTC	1 cut(s)	768
MboI	GATC	3 cut(s)	207, 351, 608
MboII	GAAGA	8 cut(s)	47, 250, 287, 361, 445, 505, 781, 836
MflI	RGATCY	2 cut(s)	351, 608
MhlI	GDGCHC	4 cut(s)	84, 240, 412, 469
MluCI	AATT	3 cut(s)	144, 245, 400
MlyI	GAGTC	3 cut(s)	420, 667, 710
MmeI	TCCRAC	2 cut(s)	207, 375
MnlI	CCTC	5 cut(s)	163, 181, 223, 265, 272
Mph1103I	ATGCAT	1 cut(s)	832
MseI	TTAA	1 cut(s)	249
MspA1I	CMGCKG	1 cut(s)	805
MspI	CCGG	3 cut(s)	47, 335, 816
MspR9I	CCNGG	1 cut(s)	335
MvnI	CGCG	2 cut(s)	747, 749
MwoI	GCNNNNNNNGC	7 cut(s)	52, 88, 97, 158, 535, 746, 805
NciI	CCSGG	1 cut(s)	335
NcoI	CCATGG	1 cut(s)	519
NdeII	GATC	3 cut(s)	207, 351, 608
NlaIII	CATG	5 cut(s)	523, 583, 665, 724, 834
NsiI	ATGCAT	1 cut(s)	832
PaeR7I	CTCGAG	1 cut(s)	263
PcsI	WCGNNNNNNNCGW	1 cut(s)	591
PfoI	TCCNGGA	1 cut(s)	333
PkrI	GCNGC	8 cut(s)	160, 179, 202, 770, 773, 801, 804, 807
PleI	GAGTC	3 cut(s)	420, 666, 709
PpsI	GAGTC	3 cut(s)	420, 666, 709
Psp124BI	GAGCTC	2 cut(s)	84, 240
PstI	CTGCAG	1 cut(s)	810
PsuI	RGATCY	2 cut(s)	351, 608
RsaI	GTAC	2 cut(s)	449, 719
RsaNI	GTAC	2 cut(s)	448, 718
SacI	GAGCTC	2 cut(s)	84, 240
SaqAI	TTAA	1 cut(s)	249
SatI	GCNGC	8 cut(s)	159, 178, 201, 769, 772, 800, 803, 806
Sau3AI	GATC	3 cut(s)	207, 351, 608
SchI	GAGTC	3 cut(s)	420, 667, 710
ScrFI	CCNGG	1 cut(s)	335
SduI	GDGCHC	4 cut(s)	84, 240, 412, 469
SetI	ASST	9 cut(s)	84, 102, 118, 224, 240, 498, 540, 716, 776
SfaNI	GCATC	1 cut(s)	817
SfcI	CTRYAG	1 cut(s)	806
Sfr274I	CTCGAG	1 cut(s)	263
SlaI	CTCGAG	1 cut(s)	263
SmlI	CTYRAG	3 cut(s)	83, 263, 440
SmoI	CTYRAG	3 cut(s)	83, 263, 440
Sse9I	AATT	3 cut(s)	144, 245, 400
SstI	GAGCTC	2 cut(s)	84, 240
StyD4I	CCNGG	1 cut(s)	333
StyI	CCWWGG	2 cut(s)	519, 681
TaaI	ACNGT	2 cut(s)	407, 781
TaiI	ACGT	1 cut(s)	224
TaqI	TCGA	4 cut(s)	105, 240, 264, 654
TasI	AATT	3 cut(s)	144, 245, 400
TatI	WGTACW	1 cut(s)	717
TauI	GCSGC	6 cut(s)	161, 180, 203, 771, 802, 805
Tru1I	TTAA	1 cut(s)	249
Tru9I	TTAA	1 cut(s)	249
TscAI	CASTG	1 cut(s)	412
TseI	GCWGC	2 cut(s)	771, 805
TspDTI	ATGAA	6 cut(s)	17, 205, 362, 596, 659, 737
TspGWI	ACGGA	1 cut(s)	393
TspRI	CASTG	1 cut(s)	412
XagI	CCTNNNNNAGG	1 cut(s)	266
XapI	RAATTY	1 cut(s)	245
XhoI	CTCGAG	1 cut(s)	263
Zsp2I	ATGCAT	1 cut(s)	832

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.