Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0012998

Physical Location & Seq

Genomic Coordinates Forward (+)

13456527 .. 13459263

Gene Structure 2737 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1172700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 870 bp

ATGAAGTTCGGTAAGAGCCTGAGCAACCAGATCGACGAGACCCTGCCGGAATGGCGGGACAAGTTCTTGTCCTACAAGGAGCTCAAGAAGAGGTTGAAGCTCATCGAACCCAAAGCCGCCGACCGCCCCGCCAAGCGCCCCAGAATTGACCCCTCCGCCGCCGATTTTAACGACGGCGGCGATAAGGAGGACATTTCCGCGGCGGAGATCAATTTCATTCAGTTGTTGGAGGACGAGCTCGAGAAATTTAACTCCTTCTTCGTCGAGAAGGAGGAAGAGTACATTATTAGATTGAAGGAGATACAAGACAGAGTAGCGAAAGCAAAGCCTTACAGCGACGAGATTATTAAGATCCGCAAGGAGATTGTGGACTTCCATGGAGAGATGGTTTTGTTGGAGAATTACAGCGCCCTCAACTATACAGGATTGGTAAAGATACTGAAGAAGTACGATAAACGAACAGGTGCTCTTATGCGCTTGCCCTTTATCCAGAAGGTCTTGCAACAGCCTTTCTTCACCACCGACTTGCTCTACAAGCTCGTGAAGGAGTGTGAGATAACGCTTGACCAGCTCTTTTCCATGAACGAACATCCCATATCAACTGAAGGATCTTATGGAGATGACGGCCGCAATCCTTCAACTTCTGCCCCTACCAAGAGTGATGGCATTAGCAGAGTATCCAGAGAACTTTCAGAGATAGAGTATATGGAGAGCTTGTACATGAAAAGTACAATATCGGCATTGCGCGCGTTGAAGGAAATTCGGAGCGGAAGCTCAACGGTTAGCGCCTTCTCGTTGCCGCCGCTGCAAACCAGCGGATTGGAAGACGCATGGAAAAAAGCCCCTGTTCTGGAACAAGTTGCTAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0010646 GO:0010647 GO:0012505 GO:0016036 GO:0031667 GO:0031668 GO:0031669 GO:0032101 GO:0032103 GO:0032104 GO:0032106 GO:0032107 GO:0032109 GO:0033554 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071496 GO:0080040 GO:0080134 GO:0080135

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SPX

Protein Analysis

290

Amino Acids

33.06

Weight (kDa)

5.74

Isoelectric Point (pI)

41.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPX	PF03105	1 - 50	3.5e-09	SPX domain
SPX	PF03105	109 - 166	3.4e-11	SPX domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012998)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20150
fragaria_vesca	FvH4_1g03200 FvH4_1g03200
malus_domestica	MD02G1031100.v1.1 MD15G1172700.v1.1
prunus_persica	Prupe.7G243500_v2.0.a1
pyrus_communis	pycom02g02600 pycom15g15440
rosa_chinensis	RchiOBHm_Chr2g0088351
rosa_laevigata	RLG00000015944
rosa_multiflora	Rmu_sc0004235.1_g000027
rosa_roxburghii	Rroxscaffold_2G00152740
rosa_rugosa	Rorug01G0483000
rosa_samantha	Rh2AG038000 Rh2BG037300 Rh2CG038400 Rh2DG037900
rosa_wichuraiana	Rw2G003010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	768
AccII	CGCG	3 cut(s)	200, 747, 749
AclWI	GGATC	2 cut(s)	346, 616
AcoI	YGGCCR	1 cut(s)	625
AcsI	RAATTY	2 cut(s)	245, 759
AcuI	CTGAAG	2 cut(s)	461, 624
AfaI	GTAC	4 cut(s)	281, 449, 719, 730
AfiI	CCNNNNNNNGG	1 cut(s)	850
AgsI	TTSAA	4 cut(s)	97, 295, 639, 754
AluBI	AGCT	7 cut(s)	82, 100, 238, 538, 571, 714, 774
AluI	AGCT	7 cut(s)	82, 100, 238, 538, 571, 714, 774
Alw21I	GWGCWC	3 cut(s)	84, 240, 469
Alw26I	GTCTC	1 cut(s)	32
AlwI	GGATC	2 cut(s)	346, 616
Ama87I	CYCGRG	1 cut(s)	239
AoxI	GGCC	1 cut(s)	625
ApeKI	GCWGC	1 cut(s)	805
ApoI	RAATTY	2 cut(s)	245, 759
AspLEI	GCGC	6 cut(s)	138, 410, 477, 747, 749, 788
AsuHPI	GGTGA	1 cut(s)	508
AvaI	CYCGRG	1 cut(s)	239
BanII	GRGCYC	2 cut(s)	84, 240
BauI	CACGAG	1 cut(s)	539
BbsI	GAAGAC	1 cut(s)	831
Bbv12I	GWGCWC	3 cut(s)	84, 240, 469
BbvI	GCAGC	1 cut(s)	792
BccI	CCATC	2 cut(s)	379, 656
BceAI	ACGGC	2 cut(s)	190, 640
BcgI	CGANNNNNNTGC	2 cut(s)	13, 47
BciVI	GTATCC	1 cut(s)	688
BcoDI	GTCTC	1 cut(s)	32
BfoI	RGCGCY	3 cut(s)	139, 411, 789
BfuI	GTATCC	1 cut(s)	688
BglI	GCCNNNNNGGC	1 cut(s)	52
BisI	GCNGC	8 cut(s)	117, 159, 178, 201, 628, 800, 803, 806
BlsI	GCNGC	8 cut(s)	118, 160, 179, 202, 629, 801, 804, 807
BmeT110I	CYCGRG	1 cut(s)	239
BpiI	GAAGAC	1 cut(s)	831
Bpu10I	CCTNAGC	1 cut(s)	20
BpuEI	CTTGAG	1 cut(s)	68
BsaI	GGTCTC	1 cut(s)	32
BsaJI	CCNNGG	2 cut(s)	198, 376
BsaXI	ACNNNNNCTCC	6 cut(s)	263, 293, 372, 402, 701, 731
Bsc4I	CCNNNNNNNGG	1 cut(s)	850
Bse3DI	GCAATG	1 cut(s)	740
BseDI	CCNNGG	2 cut(s)	198, 376
BseGI	GGATG	1 cut(s)	589
BseLI	CCNNNNNNNGG	1 cut(s)	850
BseMI	GCAATG	1 cut(s)	740
BseMII	CTCAG	1 cut(s)	11
BsePI	GCGCGC	1 cut(s)	745
BseX3I	CGGCCG	1 cut(s)	625
BseXI	GCAGC	1 cut(s)	792
Bsh1236I	CGCG	3 cut(s)	200, 747, 749
Bsh1285I	CGRYCG	2 cut(s)	124, 628
BshFI	GGCC	1 cut(s)	627
BsiEI	CGRYCG	2 cut(s)	124, 628
BsiHKAI	GWGCWC	3 cut(s)	84, 240, 469
BsiHKCI	CYCGRG	1 cut(s)	239
BsiSI	CCGG	1 cut(s)	47
BslFI	GGGAC	1 cut(s)	71
BslI	CCNNNNNNNGG	1 cut(s)	850
BsmAI	GTCTC	1 cut(s)	32
BsmFI	GGGAC	1 cut(s)	71
BsnI	GGCC	1 cut(s)	627
Bso31I	GGTCTC	1 cut(s)	32
BsoBI	CYCGRG	1 cut(s)	239
Bsp1286I	GDGCHC	3 cut(s)	84, 240, 469
Bsp1407I	TGTACA	1 cut(s)	717
Bsp143I	GATC	4 cut(s)	30, 207, 351, 608
Bsp19I	CCATGG	1 cut(s)	376
BspANI	GGCC	1 cut(s)	627
BspCNI	CTCAG	1 cut(s)	12
BspFNI	CGCG	3 cut(s)	200, 747, 749
BspPI	GGATC	2 cut(s)	346, 616
BspTNI	GGTCTC	1 cut(s)	32
BsrBI	CCGCTC	1 cut(s)	768
BsrDI	GCAATG	1 cut(s)	740
BsrGI	TGTACA	1 cut(s)	717
BssECI	CCNNGG	2 cut(s)	198, 376
BssHII	GCGCGC	1 cut(s)	745
BssMI	GATC	4 cut(s)	30, 207, 351, 608
BssSI	CACGAG	1 cut(s)	539
BssT1I	CCWWGG	1 cut(s)	376
Bst2BI	CACGAG	1 cut(s)	539
Bst4CI	ACNGT	1 cut(s)	781
Bst6I	CTCTTC	2 cut(s)	83, 270
BstAUI	TGTACA	1 cut(s)	717
BstC8I	GCNNGC	2 cut(s)	479, 747
BstDEI	CTNAG	2 cut(s)	20, 864
BstDSI	CCRYGG	2 cut(s)	198, 376
BstF5I	GGATG	1 cut(s)	589
BstFNI	CGCG	3 cut(s)	200, 747, 749
BstH2I	RGCGCY	3 cut(s)	139, 411, 789
BstHHI	GCGC	6 cut(s)	138, 410, 477, 747, 749, 788
BstKTI	GATC	4 cut(s)	33, 210, 354, 611
BstMAI	GTCTC	1 cut(s)	32
BstMBI	GATC	4 cut(s)	30, 207, 351, 608
BstMCI	CGRYCG	2 cut(s)	124, 628
BstMWI	GCNNNNNNNGC	5 cut(s)	52, 535, 568, 746, 805
BstUI	CGCG	3 cut(s)	200, 747, 749
BstV1I	GCAGC	1 cut(s)	792
BstV2I	GAAGAC	1 cut(s)	831
BstX2I	RGATCY	2 cut(s)	351, 608
BstXI	CCANNNNNNTGG	1 cut(s)	820
BstYI	RGATCY	2 cut(s)	351, 608
BstZI	CGGCCG	1 cut(s)	625
BsuI	GTATCC	1 cut(s)	688
BsuRI	GGCC	1 cut(s)	627
BtgI	CCRYGG	2 cut(s)	198, 376
BtsCI	GGATG	1 cut(s)	589
Cac8I	GCNNGC	2 cut(s)	479, 747
CfoI	GCGC	6 cut(s)	138, 410, 477, 747, 749, 788
Cfr42I	CCGCGG	1 cut(s)	201
CseI	GACGC	1 cut(s)	836
Csp6I	GTAC	4 cut(s)	280, 448, 718, 729
CviAII	CATG	4 cut(s)	377, 580, 721, 831
CviQI	GTAC	4 cut(s)	280, 448, 718, 729
DdeI	CTNAG	2 cut(s)	20, 864
DpnI	GATC	4 cut(s)	32, 209, 353, 610
DpnII	GATC	4 cut(s)	30, 207, 351, 608
EaeI	YGGCCR	1 cut(s)	625
EagI	CGGCCG	1 cut(s)	625
Eam1104I	CTCTTC	2 cut(s)	83, 270
EarI	CTCTTC	2 cut(s)	83, 270
EciI	GGCGGA	2 cut(s)	145, 218
Ecl136II	GAGCTC	2 cut(s)	82, 238
EclXI	CGGCCG	1 cut(s)	625
Eco130I	CCWWGG	1 cut(s)	376
Eco24I	GRGCYC	2 cut(s)	84, 240
Eco31I	GGTCTC	1 cut(s)	32
Eco52I	CGGCCG	1 cut(s)	625
Eco53kI	GAGCTC	2 cut(s)	82, 238
Eco57I	CTGAAG	2 cut(s)	461, 624
Eco88I	CYCGRG	1 cut(s)	239
EcoICRI	GAGCTC	2 cut(s)	82, 238
EcoT14I	CCWWGG	1 cut(s)	376
EcoT38I	GRGCYC	2 cut(s)	84, 240
ErhI	CCWWGG	1 cut(s)	376
FaeI	CATG	4 cut(s)	380, 583, 724, 834
FaqI	GGGAC	1 cut(s)	71
FatI	CATG	4 cut(s)	376, 579, 720, 830
FauI	CCCGC	2 cut(s)	48, 136
Fnu4HI	GCNGC	8 cut(s)	117, 159, 178, 201, 628, 800, 803, 806
FokI	GGATG	1 cut(s)	576
FriOI	GRGCYC	2 cut(s)	84, 240
Fsp4HI	GCNGC	8 cut(s)	117, 159, 178, 201, 628, 800, 803, 806
GlaI	GCGC	6 cut(s)	137, 409, 476, 746, 748, 787
GluI	GCNGC	8 cut(s)	117, 159, 178, 201, 628, 800, 803, 806
HaeII	RGCGCY	3 cut(s)	139, 411, 789
HaeIII	GGCC	1 cut(s)	627
HapII	CCGG	1 cut(s)	47
HgaI	GACGC	1 cut(s)	836
HhaI	GCGC	6 cut(s)	138, 410, 477, 747, 749, 788
Hin1II	CATG	4 cut(s)	380, 583, 724, 834
Hin6I	GCGC	6 cut(s)	136, 408, 475, 745, 747, 786
HinP1I	GCGC	6 cut(s)	136, 408, 475, 745, 747, 786
HpaII	CCGG	1 cut(s)	47
HphI	GGTGA	1 cut(s)	508
Hpy166II	GTNNAC	1 cut(s)	370
Hpy188I	TCNGA	2 cut(s)	694, 765
Hpy188III	TCNNGA	7 cut(s)	85, 241, 265, 490, 541, 681, 851
Hpy8I	GTNNAC	1 cut(s)	370
Hpy99I	CGWCG	4 cut(s)	38, 176, 266, 341
HpyAV	CCTTC	9 cut(s)	262, 265, 289, 487, 538, 599, 645, 748, 799
HpyCH4III	ACNGT	1 cut(s)	781
HpyCH4V	TGCA	2 cut(s)	502, 808
HpyF10VI	GCNNNNNNNGC	5 cut(s)	52, 535, 568, 746, 805
HpyF3I	CTNAG	2 cut(s)	20, 864
Hsp92II	CATG	4 cut(s)	380, 583, 724, 834
HspAI	GCGC	6 cut(s)	136, 408, 475, 745, 747, 786
KspI	CCGCGG	1 cut(s)	201
Kzo9I	GATC	4 cut(s)	30, 207, 351, 608
LmnI	GCTCC	2 cut(s)	79, 765
Lsp1109I	GCAGC	1 cut(s)	792
MalI	GATC	4 cut(s)	32, 209, 353, 610
MbiI	CCGCTC	1 cut(s)	768
MboI	GATC	4 cut(s)	30, 207, 351, 608
MboII	GAAGA	6 cut(s)	100, 250, 287, 454, 505, 836
MflI	RGATCY	2 cut(s)	351, 608
MhlI	GDGCHC	3 cut(s)	84, 240, 469
MluCI	AATT	5 cut(s)	144, 211, 245, 400, 759
MmeI	TCCRAC	2 cut(s)	207, 375
MnlI	CCTC	6 cut(s)	84, 163, 181, 223, 265, 422
MseI	TTAA	3 cut(s)	168, 249, 348
MspA1I	CMGCKG	3 cut(s)	200, 805, 816
MspI	CCGG	1 cut(s)	47
MvnI	CGCG	3 cut(s)	200, 747, 749
MwoI	GCNNNNNNNGC	5 cut(s)	52, 535, 568, 746, 805
NcoI	CCATGG	1 cut(s)	376
NdeII	GATC	4 cut(s)	30, 207, 351, 608
NlaIII	CATG	4 cut(s)	380, 583, 724, 834
PaeR7I	CTCGAG	1 cut(s)	239
PauI	GCGCGC	1 cut(s)	745
PcsI	WCGNNNNNNNCGW	1 cut(s)	177
PkrI	GCNGC	8 cut(s)	118, 160, 179, 202, 629, 801, 804, 807
Psp124BI	GAGCTC	2 cut(s)	84, 240
PsuI	RGATCY	2 cut(s)	351, 608
PteI	GCGCGC	1 cut(s)	745
RsaI	GTAC	4 cut(s)	281, 449, 719, 730
RsaNI	GTAC	4 cut(s)	280, 448, 718, 729
SacI	GAGCTC	2 cut(s)	84, 240
SacII	CCGCGG	1 cut(s)	201
SaqAI	TTAA	3 cut(s)	168, 249, 348
SatI	GCNGC	8 cut(s)	117, 159, 178, 201, 628, 800, 803, 806
Sau3AI	GATC	4 cut(s)	30, 207, 351, 608
SduI	GDGCHC	3 cut(s)	84, 240, 469
Sfr274I	CTCGAG	1 cut(s)	239
Sfr303I	CCGCGG	1 cut(s)	201
SgrBI	CCGCGG	1 cut(s)	201
SlaI	CTCGAG	1 cut(s)	239
SmlI	CTYRAG	2 cut(s)	83, 239
SmoI	CTYRAG	2 cut(s)	83, 239
Sse9I	AATT	5 cut(s)	144, 211, 245, 400, 759
SstI	GAGCTC	2 cut(s)	84, 240
StyI	CCWWGG	1 cut(s)	376
TaaI	ACNGT	1 cut(s)	781
TaqI	TCGA	4 cut(s)	33, 105, 240, 264
TasI	AATT	5 cut(s)	144, 211, 245, 400, 759
TatI	WGTACW	3 cut(s)	279, 717, 728
TauI	GCSGC	7 cut(s)	119, 161, 180, 203, 630, 802, 805
Tru1I	TTAA	3 cut(s)	168, 249, 348
Tru9I	TTAA	3 cut(s)	168, 249, 348
TseI	GCWGC	1 cut(s)	805
TspDTI	ATGAA	4 cut(s)	17, 205, 596, 737
XapI	RAATTY	2 cut(s)	245, 759
XhoI	CTCGAG	1 cut(s)	239

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.