RLG00000015944

SPX domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012998
Physical Location & Seq
Forward (+)
2671420 .. 2673361
1942 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015944

Sequence Viewer

Length: 846 bp
ATGAAGTTCGGCAAGAGCCTGAGTAGCCAGATCGACGAGACCCTGCCGGAATGGCGCGACAAGTTCCTCTCCTACAAGGAGCTGAAGAAGCGCCTCAAGCTCATCGAGCCCAAAGGCGGCGGCGGCGACGACTCCTCGACCCGCCCCGCCAAGCGCCCCAGGGTCGACGGCGACGACATGTCGTCCGAGGAGATCAATTTCATTCAGCTCCTGGAAGACGAGCTGGAGAAGTTCAATTCCTTCTTCGTCGAAAAGGAAGAAGAGTATATTATTAGATTGAAGGAGATACAAGATAGTGTGGCGAAAGTGAAGCATTCGACTGAAGAGGTTATGAAGATACGCAAGGAGATAGTGGATTTTCATGGAGAGATGGTTTTGTTGGAGAATTACAGTGCCCTCAACTATACAGGACTGGTAAAGATACTCAAGAAGTACGATAAAAGAACTGGTGCTCTTATGCGCTTGCCCTTTATTCAGAAGGTCCTGCGACAACCTTTCTTCACCACTGACTTGCTTTACAAGCTTGTGAAGGAGTGCGAGTGCACCCTTGACCAGCTGTTTGCAATGTGTGAACCGCCAGCTTCAAATGAAGCATCTGATGATGCAGATGATGGTCCCTCAACTTCCAAGAGTAATGGACTTAGCAGAGTAAACAAGGAACTTGCAGAGATAGAGTACATCGAGAGCTTATACATGAAAAGCACTATATCGGCATTGCGAGCTTTGAAAGAAATTAGAAGCGGAAGCTCAACTGTGAGCGCATTTTCACTGCCACCACTGCAATTAAGTGGATTGGAAGAAGATGCCTGGAAAAAGGTCCCTGTTCTGGAACAGCTTGCTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPX

Protein Analysis

282

Amino Acids

32.04

Weight (kDa)

5.34

Isoelectric Point (pI)

54.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPX PF03105 1 - 36 8.5e-09 SPX domain
SPX PF03105 107 - 161 3.1e-11 SPX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012998)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20150
fragaria_vesca FvH4_1g03200 FvH4_1g03200
malus_domestica MD02G1031100.v1.1 MD15G1172700.v1.1
prunus_persica Prupe.7G243500_v2.0.a1
pyrus_communis pycom02g02600 pycom15g15440
rosa_chinensis RchiOBHm_Chr2g0088351
rosa_laevigata RLG00000015944
rosa_multiflora Rmu_sc0004235.1_g000027
rosa_roxburghii Rroxscaffold_2G00152740
rosa_rugosa Rorug01G0483000
rosa_samantha Rh2AG038000 Rh2BG037300 Rh2CG038400 Rh2DG037900
rosa_wichuraiana Rw2G003010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 165
AccII CGCG 1 cut(s) 57
AciI CCGC 7 cut(s) 117, 120, 123, 142, 147, 575, 741
AcuI CTGAAG 2 cut(s) 104, 342
AfaI GTAC 2 cut(s) 434, 677
AfiI CCNNNNNNNGG 2 cut(s) 116, 826
AflIII ACRYGT 1 cut(s) 177
AgsI TTSAA 4 cut(s) 235, 280, 585, 727
AhdI GACNNNNNGTC 2 cut(s) 178, 181
AjnI CCWGG 3 cut(s) 158, 210, 806
Alw21I GWGCWC 2 cut(s) 454, 545
Alw26I GTCTC 1 cut(s) 32
Alw44I GTGCAC 1 cut(s) 541
AlwNI CAGNNNCTG 1 cut(s) 211
ApaLI GTGCAC 1 cut(s) 541
ArsI GACNNNNNNTTYG 2 cut(s) 542, 574
AspLEI GCGC 5 cut(s) 57, 93, 156, 462, 761
AspS9I GGNCC 3 cut(s) 481, 614, 817
AsuHPI GGTGA 1 cut(s) 493
AvaII GGWCC 3 cut(s) 481, 614, 817
BaeGI GKGCMC 2 cut(s) 397, 545
BanII GRGCYC 1 cut(s) 111
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 2 cut(s) 454, 545
BccI CCATC 2 cut(s) 364, 605
BceAI ACGGC 1 cut(s) 184
BciT130I CCWGG 3 cut(s) 160, 212, 808
BcoDI GTCTC 1 cut(s) 32
BfoI RGCGCY 2 cut(s) 94, 157
BglI GCCNNNNNGGC 1 cut(s) 52
BisI GCNGC 3 cut(s) 118, 121, 124
BlsI GCNGC 3 cut(s) 119, 122, 125
Bme1390I CCNGG 3 cut(s) 160, 212, 808
Bme18I GGWCC 3 cut(s) 481, 614, 817
BmeRI GACNNNNNGTC 2 cut(s) 178, 181
BmgT120I GGNCC 3 cut(s) 481, 614, 817
BmiI GGNNCC 2 cut(s) 616, 819
BmrFI CCNGG 3 cut(s) 160, 212, 808
BmsI GCATC 3 cut(s) 592, 602, 793
BpiI GAAGAC 1 cut(s) 222
BpmI CTGGAG 1 cut(s) 245
BpuEI CTTGAG 2 cut(s) 80, 410
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 3 cut(s) 158, 159, 186
BsaXI ACNNNNNCTCC 4 cut(s) 357, 387, 524, 554
Bsc4I CCNNNNNNNGG 2 cut(s) 116, 826
Bse1I ACTGG 2 cut(s) 417, 451
Bse3DI GCAATG 2 cut(s) 570, 713
BseBI CCWGG 3 cut(s) 160, 212, 808
BseDI CCNNGG 3 cut(s) 158, 159, 186
BseLI CCNNNNNNNGG 2 cut(s) 116, 826
BseMI GCAATG 2 cut(s) 570, 713
BseMII CTCAG 1 cut(s) 11
BseNI ACTGG 2 cut(s) 417, 451
BseRI GAGGAG 2 cut(s) 124, 203
BseSI GKGCMC 2 cut(s) 397, 545
Bsh1236I CGCG 1 cut(s) 57
BsiHKAI GWGCWC 2 cut(s) 454, 545
BsiSI CCGG 1 cut(s) 47
BslFI GGGAC 2 cut(s) 600, 803
BslI CCNNNNNNNGG 2 cut(s) 116, 826
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 2 cut(s) 600, 803
BsmI GAATGC 1 cut(s) 313
Bso31I GGTCTC 1 cut(s) 32
Bsp1286I GDGCHC 4 cut(s) 111, 397, 454, 545
Bsp143I GATC 2 cut(s) 30, 192
BspACI CCGC 7 cut(s) 117, 120, 123, 142, 147, 575, 741
BspCNI CTCAG 1 cut(s) 12
BspFNI CGCG 1 cut(s) 57
BspLI GGNNCC 2 cut(s) 616, 819
BspTNI GGTCTC 1 cut(s) 32
BsrDI GCAATG 2 cut(s) 570, 713
BsrI ACTGG 2 cut(s) 417, 451
BssECI CCNNGG 3 cut(s) 158, 159, 186
BssMI GATC 2 cut(s) 30, 192
Bst2UI CCWGG 3 cut(s) 160, 212, 808
Bst4CI ACNGT 2 cut(s) 392, 754
Bst6I CTCTTC 2 cut(s) 255, 318
BstC8I GCNNGC 4 cut(s) 464, 579, 720, 837
BstDEI CTNAG 3 cut(s) 20, 641, 840
BstFNI CGCG 1 cut(s) 57
BstH2I RGCGCY 2 cut(s) 94, 157
BstHHI GCGC 5 cut(s) 57, 93, 156, 462, 761
BstKTI GATC 2 cut(s) 33, 195
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 2 cut(s) 30, 192
BstMWI GCNNNNNNNGC 9 cut(s) 24, 52, 88, 97, 106, 123, 520, 719, 778
BstNI CCWGG 3 cut(s) 160, 212, 808
BstNSI RCATGY 1 cut(s) 181
BstSCI CCNGG 3 cut(s) 158, 210, 806
BstSLI GKGCMC 2 cut(s) 397, 545
BstUI CGCG 1 cut(s) 57
BstV2I GAAGAC 1 cut(s) 222
BtsI GCAGTG 2 cut(s) 767, 776
BtsIMutI CAGTG 4 cut(s) 397, 504, 767, 776
Cac8I GCNNGC 4 cut(s) 464, 579, 720, 837
CaiI CAGNNNCTG 1 cut(s) 211
CfoI GCGC 5 cut(s) 57, 93, 156, 462, 761
Cfr13I GGNCC 3 cut(s) 481, 614, 817
Csp6I GTAC 2 cut(s) 433, 676
CviAII CATG 3 cut(s) 178, 362, 694
CviQI GTAC 2 cut(s) 433, 676
DdeI CTNAG 3 cut(s) 20, 641, 840
DpnI GATC 2 cut(s) 32, 194
DpnII GATC 2 cut(s) 30, 192
DriI GACNNNNNGTC 2 cut(s) 178, 181
Eam1104I CTCTTC 2 cut(s) 255, 318
Eam1105I GACNNNNNGTC 2 cut(s) 178, 181
EarI CTCTTC 2 cut(s) 255, 318
Eco24I GRGCYC 1 cut(s) 111
Eco31I GGTCTC 1 cut(s) 32
Eco47I GGWCC 3 cut(s) 481, 614, 817
Eco57I CTGAAG 2 cut(s) 104, 342
EcoO109I RGGNCCY 2 cut(s) 481, 817
EcoRII CCWGG 3 cut(s) 158, 210, 806
EcoT38I GRGCYC 1 cut(s) 111
FaeI CATG 3 cut(s) 181, 365, 697
FaiI YATR 9 cut(s) 179, 267, 332, 363, 405, 458, 691, 695, 707
FaqI GGGAC 2 cut(s) 600, 803
FatI CATG 3 cut(s) 177, 361, 693
FauI CCCGC 2 cut(s) 149, 154
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 3 cut(s) 118, 121, 124
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 3 cut(s) 118, 121, 124
GlaI GCGC 5 cut(s) 56, 92, 155, 461, 760
GluI GCNGC 3 cut(s) 118, 121, 124
GsuI CTGGAG 1 cut(s) 245
HaeII RGCGCY 2 cut(s) 94, 157
HapII CCGG 1 cut(s) 47
HhaI GCGC 5 cut(s) 57, 93, 156, 462, 761
Hin1II CATG 3 cut(s) 181, 365, 697
Hin6I GCGC 5 cut(s) 55, 91, 154, 460, 759
HinP1I GCGC 5 cut(s) 55, 91, 154, 460, 759
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 1 cut(s) 131
HpaII CCGG 1 cut(s) 47
HphI GGTGA 1 cut(s) 493
Hpy166II GTNNAC 4 cut(s) 166, 543, 572, 652
Hpy188I TCNGA 3 cut(s) 187, 477, 598
Hpy188III TCNNGA 3 cut(s) 427, 682, 827
Hpy8I GTNNAC 4 cut(s) 166, 543, 572, 652
Hpy99I CGWCG 5 cut(s) 38, 131, 170, 176, 251
HpyAV CCTTC 4 cut(s) 250, 274, 472, 523
HpyCH4III ACNGT 2 cut(s) 392, 754
HpyCH4V TGCA 5 cut(s) 543, 563, 605, 665, 781
HpyF10VI GCNNNNNNNGC 9 cut(s) 24, 52, 88, 97, 106, 123, 520, 719, 778
HpyF3I CTNAG 3 cut(s) 20, 641, 840
Hsp92II CATG 3 cut(s) 181, 365, 697
HspAI GCGC 5 cut(s) 55, 91, 154, 460, 759
Kzo9I GATC 2 cut(s) 30, 192
LmnI GCTCC 2 cut(s) 79, 213
LweI GCATC 3 cut(s) 592, 602, 793
MalI GATC 2 cut(s) 32, 194
MboI GATC 2 cut(s) 30, 192
MhlI GDGCHC 4 cut(s) 111, 397, 454, 545
MluCI AATT 5 cut(s) 196, 235, 385, 732, 782
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 1 cut(s) 360
MnlI CCTC 7 cut(s) 77, 104, 145, 181, 319, 407, 628
MseI TTAA 1 cut(s) 785
MspA1I CMGCKG 1 cut(s) 556
MspI CCGG 1 cut(s) 47
MspR9I CCNGG 3 cut(s) 160, 212, 808
Mva1269I GAATGC 1 cut(s) 313
MvaI CCWGG 3 cut(s) 160, 212, 808
MvnI CGCG 1 cut(s) 57
MwoI GCNNNNNNNGC 9 cut(s) 24, 52, 88, 97, 106, 123, 520, 719, 778
NdeII GATC 2 cut(s) 30, 192
NlaIII CATG 3 cut(s) 181, 365, 697
NlaIV GGNNCC 2 cut(s) 616, 819
NspI RCATGY 1 cut(s) 181
PasI CCCWGGG 1 cut(s) 159
PciI ACATGT 1 cut(s) 177
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PctI GAATGC 1 cut(s) 313
PfoI TCCNGGA 1 cut(s) 210
PkrI GCNGC 3 cut(s) 119, 122, 125
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
PpuMI RGGWCCY 2 cut(s) 481, 817
PscI ACATGT 1 cut(s) 177
Psp5II RGGWCCY 2 cut(s) 481, 817
Psp6I CCWGG 3 cut(s) 158, 210, 806
PspGI CCWGG 3 cut(s) 158, 210, 806
PspN4I GGNNCC 2 cut(s) 616, 819
PspPI GGNCC 3 cut(s) 481, 614, 817
PspPPI RGGWCCY 2 cut(s) 481, 817
PstNI CAGNNNCTG 1 cut(s) 211
PvuII CAGCTG 1 cut(s) 556
RsaI GTAC 2 cut(s) 434, 677
RsaNI GTAC 2 cut(s) 433, 676
SalI GTCGAC 1 cut(s) 164
SaqAI TTAA 1 cut(s) 785
SatI GCNGC 3 cut(s) 118, 121, 124
Sau3AI GATC 2 cut(s) 30, 192
Sau96I GGNCC 3 cut(s) 481, 614, 817
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 3 cut(s) 160, 212, 808
SduI GDGCHC 4 cut(s) 111, 397, 454, 545
SfaNI GCATC 3 cut(s) 592, 602, 793
SinI GGWCC 3 cut(s) 481, 614, 817
SmlI CTYRAG 2 cut(s) 95, 425
SmoI CTYRAG 2 cut(s) 95, 425
Sse9I AATT 5 cut(s) 196, 235, 385, 732, 782
SsiI CCGC 7 cut(s) 117, 120, 123, 142, 147, 575, 741
StyD4I CCNGG 3 cut(s) 158, 210, 806
TaaI ACNGT 2 cut(s) 392, 754
TaqI TCGA 7 cut(s) 33, 105, 137, 165, 249, 317, 681
TasI AATT 5 cut(s) 196, 235, 385, 732, 782
TatI WGTACW 1 cut(s) 675
TauI GCSGC 3 cut(s) 120, 123, 126
Tru1I TTAA 1 cut(s) 785
Tru9I TTAA 1 cut(s) 785
TscAI CASTG 4 cut(s) 397, 511, 774, 783
TspDTI ATGAA 6 cut(s) 17, 190, 347, 350, 603, 710
TspRI CASTG 4 cut(s) 397, 511, 774, 783
VneI GTGCAC 1 cut(s) 541
VpaK11BI GGWCC 3 cut(s) 481, 614, 817
XceI RCATGY 1 cut(s) 181
XmiI GTMKAC 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.