Rroxscaffold_2G00152740

SPX domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012998
Physical Location & Seq
Reverse (-)
89587709 .. 89589936
2228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00152740.1

Sequence Viewer

Length: 837 bp
ATGAAGTTTGGCAAGAGCCTGAGCAGCCAGATCGACGAGACCTTGCCGGAATGGCGCGACAAGTTCCTGTCCTACAAGGAGCTCAAGAAGCGCCTCAAGCTCATCGAGCCGAAAGGAGGCGACGCTGGAACCAGGCCAGCCAAGCGCCTCAGGCTCCACGGCGACGACATGTCGTCCGAGGAGATCAATTTCATTCAGCTCCTCGAGGATGAGCTCGAGAAGTTCAATTCCTTCTTTGTCGAAAAGGAAGAAGAGTATATTATTAGATTGAAGGAAATACAAGACAGTGTGGCGAAAGCAAAGCATTCGACTGAAGAGATTATGAAGATACGCAAGGAGATAGTGGATTTTCATGGAGAGATGGTTTTGTTGGAGAATTATAGTGCCCTCAACTATACAGGACTGGTAAAGATACTGAAGAAGTACGATAAAAGAACTGGTGCTCTTATGCGCTTGCCTTTTATTCAGAAGGTCCTGCAACAACCTTTCTTCACCACTGACTTGCTTTACAAGCTTGTGAAGGAGTGCGAGTGCACGCTCGACCAGCTGTTTGCAATGTGTGAACCGCCAGCTTCAAATGAAGCATCTGATGACGGGGATGATGGTCCCTCAACGTCCAAGAGTGATGGACTTAGCAGAGTAAACAAGGAACTTGCAGAGATAGAGTACATCGAGAGCTTATATATGAAAAGCACTATATCGGCGTTGCGAGCTTTGAAAGAAATTAGAAGTGGAAGCTCTACTGTGAGCGCATTTTCATTGCCACCACTGCAATTAAGTGTATTAGAAGAAGATGCCTGGAAAAAGGTCCCCGTTCTGGAACAGCTTGCTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPX

Protein Analysis

278

Amino Acids

31.8

Weight (kDa)

5.42

Isoelectric Point (pI)

55.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPX PF03105 1 - 35 1.4e-08 SPX domain
SPX PF03105 105 - 158 2.9e-11 SPX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012998)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20150
fragaria_vesca FvH4_1g03200 FvH4_1g03200
malus_domestica MD02G1031100.v1.1 MD15G1172700.v1.1
prunus_persica Prupe.7G243500_v2.0.a1
pyrus_communis pycom02g02600 pycom15g15440
rosa_chinensis RchiOBHm_Chr2g0088351
rosa_laevigata RLG00000015944
rosa_multiflora Rmu_sc0004235.1_g000027
rosa_roxburghii Rroxscaffold_2G00152740
rosa_rugosa Rorug01G0483000
rosa_samantha Rh2AG038000 Rh2BG037300 Rh2CG038400 Rh2DG037900
rosa_wichuraiana Rw2G003010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 203
AccII CGCG 1 cut(s) 57
AciI CCGC 1 cut(s) 566
AcuI CTGAAG 2 cut(s) 333, 437
AfaI GTAC 2 cut(s) 425, 668
AfiI CCNNNNNNNGG 2 cut(s) 116, 817
AflIII ACRYGT 1 cut(s) 168
AgsI TTSAA 4 cut(s) 226, 271, 576, 718
AhdI GACNNNNNGTC 2 cut(s) 169, 172
AjnI CCWGG 2 cut(s) 131, 797
Alw21I GWGCWC 4 cut(s) 84, 216, 445, 536
Alw26I GTCTC 1 cut(s) 32
Alw44I GTGCAC 1 cut(s) 532
Ama87I CYCGRG 2 cut(s) 203, 215
AoxI GGCC 1 cut(s) 134
ApaLI GTGCAC 1 cut(s) 532
ApeKI GCWGC 1 cut(s) 24
ArsI GACNNNNNNTTYG 2 cut(s) 533, 565
AspLEI GCGC 5 cut(s) 57, 93, 147, 453, 752
AspS9I GGNCC 3 cut(s) 472, 605, 808
AsuHPI GGTGA 1 cut(s) 484
AvaI CYCGRG 2 cut(s) 203, 215
AvaII GGWCC 3 cut(s) 472, 605, 808
AxyI CCTNAGG 1 cut(s) 149
BaeGI GKGCMC 2 cut(s) 388, 536
BanII GRGCYC 2 cut(s) 84, 216
Bbv12I GWGCWC 4 cut(s) 84, 216, 445, 536
BbvI GCAGC 1 cut(s) 36
BccI CCATC 3 cut(s) 355, 596, 620
BceAI ACGGC 1 cut(s) 175
BcgI CGANNNNNNTGC 4 cut(s) 13, 47, 288, 322
BciT130I CCWGG 2 cut(s) 133, 799
BcoDI GTCTC 1 cut(s) 32
BfoI RGCGCY 2 cut(s) 94, 148
BglI GCCNNNNNGGC 1 cut(s) 52
BisI GCNGC 1 cut(s) 25
BlsI GCNGC 1 cut(s) 26
Bme1390I CCNGG 2 cut(s) 133, 799
Bme18I GGWCC 3 cut(s) 472, 605, 808
BmeRI GACNNNNNGTC 2 cut(s) 169, 172
BmeT110I CYCGRG 2 cut(s) 203, 215
BmgT120I GGNCC 3 cut(s) 472, 605, 808
BmiI GGNNCC 4 cut(s) 130, 155, 607, 810
BmrFI CCNGG 2 cut(s) 133, 799
BmsI GCATC 2 cut(s) 593, 784
Bpu10I CCTNAGC 1 cut(s) 20
BpuEI CTTGAG 2 cut(s) 68, 80
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 2 cut(s) 157, 177
BsaXI ACNNNNNCTCC 4 cut(s) 348, 378, 515, 545
Bsc4I CCNNNNNNNGG 2 cut(s) 116, 817
Bse1I ACTGG 2 cut(s) 408, 442
Bse21I CCTNAGG 1 cut(s) 149
Bse3DI GCAATG 2 cut(s) 561, 758
BseBI CCWGG 2 cut(s) 133, 799
BseDI CCNNGG 2 cut(s) 157, 177
BseGI GGATG 2 cut(s) 214, 604
BseLI CCNNNNNNNGG 2 cut(s) 116, 817
BseMI GCAATG 2 cut(s) 561, 758
BseMII CTCAG 2 cut(s) 11, 163
BseNI ACTGG 2 cut(s) 408, 442
BseRI GAGGAG 2 cut(s) 191, 194
BseSI GKGCMC 2 cut(s) 388, 536
BseXI GCAGC 1 cut(s) 36
Bsh1236I CGCG 1 cut(s) 57
BshFI GGCC 1 cut(s) 136
BsiHKAI GWGCWC 4 cut(s) 84, 216, 445, 536
BsiHKCI CYCGRG 2 cut(s) 203, 215
BsiSI CCGG 1 cut(s) 47
BslFI GGGAC 2 cut(s) 591, 794
BslI CCNNNNNNNGG 2 cut(s) 116, 817
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 2 cut(s) 591, 794
BsmI GAATGC 1 cut(s) 304
BsnI GGCC 1 cut(s) 136
Bso31I GGTCTC 1 cut(s) 32
BsoBI CYCGRG 2 cut(s) 203, 215
Bsp1286I GDGCHC 5 cut(s) 84, 216, 388, 445, 536
Bsp143I GATC 2 cut(s) 30, 183
BspACI CCGC 1 cut(s) 566
BspANI GGCC 1 cut(s) 136
BspCNI CTCAG 2 cut(s) 12, 162
BspFNI CGCG 1 cut(s) 57
BspLI GGNNCC 4 cut(s) 130, 155, 607, 810
BspTNI GGTCTC 1 cut(s) 32
BsrDI GCAATG 2 cut(s) 561, 758
BsrI ACTGG 2 cut(s) 408, 442
BssECI CCNNGG 2 cut(s) 157, 177
BssMI GATC 2 cut(s) 30, 183
Bst2UI CCWGG 2 cut(s) 133, 799
Bst4CI ACNGT 2 cut(s) 287, 745
Bst6I CTCTTC 2 cut(s) 246, 309
BstC8I GCNNGC 6 cut(s) 138, 455, 536, 570, 711, 828
BstDEI CTNAG 4 cut(s) 20, 149, 632, 831
BstDSI CCRYGG 1 cut(s) 157
BstF5I GGATG 2 cut(s) 214, 604
BstFNI CGCG 1 cut(s) 57
BstH2I RGCGCY 2 cut(s) 94, 148
BstHHI GCGC 5 cut(s) 57, 93, 147, 453, 752
BstKTI GATC 2 cut(s) 33, 186
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 2 cut(s) 30, 183
BstNI CCWGG 2 cut(s) 133, 799
BstNSI RCATGY 1 cut(s) 172
BstSCI CCNGG 2 cut(s) 131, 797
BstSLI GKGCMC 2 cut(s) 388, 536
BstUI CGCG 1 cut(s) 57
BstV1I GCAGC 1 cut(s) 36
Bsu36I CCTNAGG 1 cut(s) 149
BsuRI GGCC 1 cut(s) 136
BtgI CCRYGG 1 cut(s) 157
BtsCI GGATG 2 cut(s) 214, 604
BtsI GCAGTG 1 cut(s) 767
BtsIMutI CAGTG 3 cut(s) 292, 495, 767
Cac8I GCNNGC 6 cut(s) 138, 455, 536, 570, 711, 828
CfoI GCGC 5 cut(s) 57, 93, 147, 453, 752
Cfr13I GGNCC 3 cut(s) 472, 605, 808
CseI GACGC 1 cut(s) 131
Csp6I GTAC 2 cut(s) 424, 667
CviAII CATG 2 cut(s) 169, 353
CviQI GTAC 2 cut(s) 424, 667
DdeI CTNAG 4 cut(s) 20, 149, 632, 831
DpnI GATC 2 cut(s) 32, 185
DpnII GATC 2 cut(s) 30, 183
DriI GACNNNNNGTC 2 cut(s) 169, 172
Eam1104I CTCTTC 2 cut(s) 246, 309
Eam1105I GACNNNNNGTC 2 cut(s) 169, 172
EarI CTCTTC 2 cut(s) 246, 309
Ecl136II GAGCTC 2 cut(s) 82, 214
Eco24I GRGCYC 2 cut(s) 84, 216
Eco31I GGTCTC 1 cut(s) 32
Eco47I GGWCC 3 cut(s) 472, 605, 808
Eco53kI GAGCTC 2 cut(s) 82, 214
Eco57I CTGAAG 2 cut(s) 333, 437
Eco81I CCTNAGG 1 cut(s) 149
Eco88I CYCGRG 2 cut(s) 203, 215
EcoICRI GAGCTC 2 cut(s) 82, 214
EcoO109I RGGNCCY 2 cut(s) 472, 808
EcoRII CCWGG 2 cut(s) 131, 797
EcoT38I GRGCYC 2 cut(s) 84, 216
FaeI CATG 2 cut(s) 172, 356
FaqI GGGAC 2 cut(s) 591, 794
FatI CATG 2 cut(s) 168, 352
Fnu4HI GCNGC 1 cut(s) 25
FokI GGATG 2 cut(s) 221, 611
FriOI GRGCYC 2 cut(s) 84, 216
Fsp4HI GCNGC 1 cut(s) 25
GlaI GCGC 5 cut(s) 56, 92, 146, 452, 751
GluI GCNGC 1 cut(s) 25
HaeII RGCGCY 2 cut(s) 94, 148
HaeIII GGCC 1 cut(s) 136
HapII CCGG 1 cut(s) 47
HgaI GACGC 1 cut(s) 131
HhaI GCGC 5 cut(s) 57, 93, 147, 453, 752
Hin1II CATG 2 cut(s) 172, 356
Hin6I GCGC 5 cut(s) 55, 91, 145, 451, 750
HinP1I GCGC 5 cut(s) 55, 91, 145, 451, 750
HindIII AAGCTT 1 cut(s) 512
HpaII CCGG 1 cut(s) 47
HphI GGTGA 1 cut(s) 484
Hpy166II GTNNAC 3 cut(s) 534, 563, 643
Hpy188I TCNGA 3 cut(s) 178, 468, 589
Hpy188III TCNNGA 4 cut(s) 85, 217, 673, 818
Hpy8I GTNNAC 3 cut(s) 534, 563, 643
Hpy99I CGWCG 3 cut(s) 38, 125, 167
HpyAV CCTTC 4 cut(s) 241, 265, 463, 514
HpyCH4III ACNGT 2 cut(s) 287, 745
HpyCH4IV ACGT 1 cut(s) 614
HpyCH4V TGCA 5 cut(s) 478, 534, 554, 656, 772
HpyF3I CTNAG 4 cut(s) 20, 149, 632, 831
HpySE526I ACGT 1 cut(s) 614
Hsp92II CATG 2 cut(s) 172, 356
HspAI GCGC 5 cut(s) 55, 91, 145, 451, 750
Kzo9I GATC 2 cut(s) 30, 183
LmnI GCTCC 3 cut(s) 79, 159, 204
Lsp1109I GCAGC 1 cut(s) 36
LweI GCATC 2 cut(s) 593, 784
MaeII ACGT 1 cut(s) 614
MalI GATC 2 cut(s) 32, 185
MboI GATC 2 cut(s) 30, 183
MboII GAAGA 8 cut(s) 260, 263, 326, 337, 430, 481, 800, 803
MhlI GDGCHC 5 cut(s) 84, 216, 388, 445, 536
MluCI AATT 5 cut(s) 187, 226, 376, 723, 773
MmeI TCCRAC 1 cut(s) 351
MnlI CCTC 8 cut(s) 104, 110, 158, 172, 199, 212, 398, 619
MseI TTAA 1 cut(s) 776
MspA1I CMGCKG 1 cut(s) 547
MspI CCGG 1 cut(s) 47
MspR9I CCNGG 2 cut(s) 133, 799
Mva1269I GAATGC 1 cut(s) 304
MvaI CCWGG 2 cut(s) 133, 799
MvnI CGCG 1 cut(s) 57
NdeII GATC 2 cut(s) 30, 183
NlaIII CATG 2 cut(s) 172, 356
NlaIV GGNNCC 4 cut(s) 130, 155, 607, 810
NspI RCATGY 1 cut(s) 172
PaeR7I CTCGAG 2 cut(s) 203, 215
PciI ACATGT 1 cut(s) 168
PctI GAATGC 1 cut(s) 304
PkrI GCNGC 1 cut(s) 26
PpuMI RGGWCCY 2 cut(s) 472, 808
PscI ACATGT 1 cut(s) 168
Psp124BI GAGCTC 2 cut(s) 84, 216
Psp5II RGGWCCY 2 cut(s) 472, 808
Psp6I CCWGG 2 cut(s) 131, 797
PspGI CCWGG 2 cut(s) 131, 797
PspN4I GGNNCC 4 cut(s) 130, 155, 607, 810
PspPI GGNCC 3 cut(s) 472, 605, 808
PspPPI RGGWCCY 2 cut(s) 472, 808
PspXI VCTCGAGB 1 cut(s) 203
PvuII CAGCTG 1 cut(s) 547
RsaI GTAC 2 cut(s) 425, 668
RsaNI GTAC 2 cut(s) 424, 667
SacI GAGCTC 2 cut(s) 84, 216
SaqAI TTAA 1 cut(s) 776
SatI GCNGC 1 cut(s) 25
Sau3AI GATC 2 cut(s) 30, 183
Sau96I GGNCC 3 cut(s) 472, 605, 808
ScrFI CCNGG 2 cut(s) 133, 799
SduI GDGCHC 5 cut(s) 84, 216, 388, 445, 536
SfaNI GCATC 2 cut(s) 593, 784
Sfr274I CTCGAG 2 cut(s) 203, 215
SinI GGWCC 3 cut(s) 472, 605, 808
SlaI CTCGAG 2 cut(s) 203, 215
SmlI CTYRAG 4 cut(s) 83, 95, 203, 215
SmoI CTYRAG 4 cut(s) 83, 95, 203, 215
Sse9I AATT 5 cut(s) 187, 226, 376, 723, 773
SsiI CCGC 1 cut(s) 566
SstI GAGCTC 2 cut(s) 84, 216
StyD4I CCNGG 2 cut(s) 131, 797
TaaI ACNGT 2 cut(s) 287, 745
TaiI ACGT 1 cut(s) 617
TaqI TCGA 8 cut(s) 33, 105, 204, 216, 240, 308, 540, 672
TasI AATT 5 cut(s) 187, 226, 376, 723, 773
TatI WGTACW 1 cut(s) 666
Tru1I TTAA 1 cut(s) 776
Tru9I TTAA 1 cut(s) 776
TscAI CASTG 3 cut(s) 292, 502, 774
TseI GCWGC 1 cut(s) 24
TspDTI ATGAA 7 cut(s) 17, 181, 338, 341, 594, 701, 747
TspRI CASTG 3 cut(s) 292, 502, 774
VneI GTGCAC 1 cut(s) 532
VpaK11BI GGWCC 3 cut(s) 472, 605, 808
XceI RCATGY 1 cut(s) 172
XhoI CTCGAG 2 cut(s) 203, 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.