FvH4_1g13000

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005637
Physical Location & Seq
Reverse (-)
7169346 .. 7172189
2844 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g13000.t2

Sequence Viewer

Length: 1125 bp
ATGTCGTCGTCGTGTGCAGTTTCGACTTCAGTGAGAACAGAGCTGTTTAGTTCCGTACCTGAGAACAGGTTCACTGCACCCACAAGGCCATTTCAGTACCACAGAAGCAAATGTGTGATGAACAGGAGGAGTTTTGGTTTTAGAGGAGTTGCAGCTGCTGGGCTTTCAGTAATGGGTTCTTCATTGGCTACTGCGCCTGTTCAAGGTATTGAAAGGCTACCATTCAAAGCTGAGGGGTACAATTTTTGGACATGGAGAGGTCATAAAATTCATTATGTGGTGCAAGGAGAAGGGCCTGCTGTTGTTCTTATCCATGGTTTTGGTGCTTCCTCATTTCACTGGAGGTATAACATACCAGAGTTATCTAAAAAGTACAAGGTTTATGCTATAGACTTGCTGGGCTTCGGGTGGAGTGAAAAAGCACTGGTTGAATATGATGCCATGATTTGGAAAGATCAAGTAGTGGACTTCTTGAAGGAGATAGTGAAAGAGCCAACAGTGTTGGTTGGAAATAGCCTTGGAGGTTTTACCGCTTTGGTTGCAGCAGTCGGATTGCCTGAGCAAGTAGTTGGAGTTGCACTACTGAATTCAGCAGGACAGTTTGGAAATGCAAATAGTGAACCTGAGAAGTCTGAGGAGGAAACAGTTTTACAAAAGTTTATCATAAAGCCACTGAAGGAAGTTTTCCAGAGAGTAGTACTTGGATTTTTGTTCTGGCAAGCAAAGCAGCCCGCTCGCATTGAATCTGTTTTAAAGAGTGTGTATAAAAATACCTCCAACGTGGATGACTATCTGGTGGAATCAATAACAATGCCAGCAGCTGACCCCAATGCCGGAGAAGTCTATTACAGATTGATGACCCGGTTCATGTCAAACCAGACCAAGTACACTCTAGATAGTATCCTAAGCAAGATGTCATGCCCATTGTTGTTGCTCTGGGGTGACTTGGACCCATGGGTAGGTCCTGCCAAGGCTAACAGAATCAAAGAGTTCTATCCAAATTCGACTTTAGTAAATTTGCAGGCTGGCCACTGTCCACACGATGAAGTGCCAGAGCTTGTAAACAAGGCTTTATTAGAATGGCTATCAACTTCAACACCTGAAGCCTCTCTCCAAATAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

375

Amino Acids

41.65

Weight (kDa)

7.66

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 99 - 349 1.2e-22 alpha/beta hydrolase fold
Hydrolase_4 PF12146 100 - 331 1.8e-13 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 101 - 356 3.6e-23 Alpha/beta hydrolase family
LIDHydrolase PF10230 122 - 348 1e-05 Lipid-droplet associated hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 447
AccBSI CCGCTC 1 cut(s) 734
AciI CCGC 2 cut(s) 531, 732
AcoI YGGCCR 1 cut(s) 1027
AcsI RAATTY 4 cut(s) 267, 586, 1000, 1015
AcuI CTGAAG 3 cut(s) 12, 695, 1122
AfaI GTAC 6 cut(s) 57, 98, 239, 374, 699, 887
AfiI CCNNNNNNNGG 4 cut(s) 203, 447, 833, 959
AgsI TTSAA 7 cut(s) 203, 212, 226, 431, 475, 743, 1095
AluBI AGCT 5 cut(s) 43, 155, 230, 821, 1057
AluI AGCT 5 cut(s) 43, 155, 230, 821, 1057
AlwNI CAGNNNCTG 2 cut(s) 158, 821
AoxI GGCC 3 cut(s) 86, 293, 1027
ApeKI GCWGC 5 cut(s) 152, 155, 542, 727, 818
ApoI RAATTY 4 cut(s) 267, 586, 1000, 1015
Asp700I GAANNNNTTC 1 cut(s) 68
AspLEI GCGC 1 cut(s) 196
AspS9I GGNCC 3 cut(s) 293, 949, 962
AsuC2I CCSGG 1 cut(s) 862
AsuHPI GGTGA 1 cut(s) 953
AvaII GGWCC 2 cut(s) 949, 962
BalI TGGCCA 1 cut(s) 1029
BbvCI CCTCAGC 1 cut(s) 231
BbvI GCAGC 5 cut(s) 142, 164, 554, 739, 830
BcgI CGANNNNNNTGC 2 cut(s) 716, 750
BciVI GTATCC 1 cut(s) 911
BcnI CCSGG 1 cut(s) 862
BfaI CTAG 1 cut(s) 893
BfmI CTRYAG 1 cut(s) 387
BfuI GTATCC 1 cut(s) 911
BisI GCNGC 5 cut(s) 153, 156, 543, 728, 819
BlsI GCNGC 5 cut(s) 154, 157, 544, 729, 820
BmcAI AGTACT 1 cut(s) 699
Bme1390I CCNGG 1 cut(s) 862
Bme18I GGWCC 2 cut(s) 949, 962
BmgT120I GGNCC 3 cut(s) 293, 949, 962
BmiI GGNNCC 1 cut(s) 951
BmrFI CCNGG 1 cut(s) 862
BmsI GCATC 1 cut(s) 427
BpmI CTGGAG 1 cut(s) 361
Bpu10I CCTNAGC 3 cut(s) 231, 558, 905
BpuMI CCSGG 1 cut(s) 862
BsaBI GATNNNNATC 1 cut(s) 789
BsaJI CCNNGG 4 cut(s) 313, 517, 953, 969
BsaXI ACNNNNNCTCC 2 cut(s) 121, 151
Bsc4I CCNNNNNNNGG 4 cut(s) 203, 447, 833, 959
Bse1I ACTGG 2 cut(s) 344, 429
Bse8I GATNNNNATC 1 cut(s) 789
BseDI CCNNGG 4 cut(s) 313, 517, 953, 969
BseGI GGATG 1 cut(s) 790
BseJI GATNNNNATC 1 cut(s) 789
BseLI CCNNNNNNNGG 4 cut(s) 203, 447, 833, 959
BseMII CTCAG 5 cut(s) 51, 222, 549, 615, 624
BseNI ACTGG 2 cut(s) 344, 429
BseRI GAGGAG 3 cut(s) 142, 159, 650
BseXI GCAGC 5 cut(s) 142, 164, 554, 739, 830
BseYI CCCAGC 2 cut(s) 158, 397
BsgI GTGCAG 2 cut(s) 36, 60
BshFI GGCC 3 cut(s) 88, 295, 1029
BsiSI CCGG 2 cut(s) 834, 862
BslI CCNNNNNNNGG 4 cut(s) 203, 447, 833, 959
BsnI GGCC 3 cut(s) 88, 295, 1029
Bsp143I GATC 1 cut(s) 454
Bsp19I CCATGG 2 cut(s) 313, 953
BspACI CCGC 2 cut(s) 531, 732
BspANI GGCC 3 cut(s) 88, 295, 1029
BspCNI CTCAG 5 cut(s) 52, 223, 550, 616, 625
BspLI GGNNCC 1 cut(s) 951
BsrBI CCGCTC 1 cut(s) 734
BsrI ACTGG 2 cut(s) 344, 429
BssECI CCNNGG 4 cut(s) 313, 517, 953, 969
BssMI GATC 1 cut(s) 454
BssT1I CCWWGG 4 cut(s) 313, 517, 953, 969
Bst4CI ACNGT 4 cut(s) 499, 600, 646, 1034
BstC8I GCNNGC 7 cut(s) 297, 720, 732, 736, 816, 1023, 1027
BstDEI CTNAG 6 cut(s) 60, 231, 558, 624, 633, 905
BstDSI CCRYGG 2 cut(s) 313, 953
BstENI CCTNNNNNAGG 1 cut(s) 201
BstF5I GGATG 1 cut(s) 790
BstHHI GCGC 1 cut(s) 196
BstKTI GATC 1 cut(s) 457
BstMBI GATC 1 cut(s) 454
BstMWI GCNNNNNNNGC 2 cut(s) 539, 724
BstSCI CCNGG 1 cut(s) 860
BstSFI CTRYAG 1 cut(s) 387
BstV1I GCAGC 5 cut(s) 142, 164, 554, 739, 830
BstXI CCANNNNNNTGG 1 cut(s) 320
BsuI GTATCC 1 cut(s) 911
BsuRI GGCC 3 cut(s) 88, 295, 1029
BtgI CCRYGG 2 cut(s) 313, 953
BtsCI GGATG 1 cut(s) 790
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 7 cut(s) 36, 72, 337, 422, 504, 671, 1030
Cac8I GCNNGC 7 cut(s) 297, 720, 732, 736, 816, 1023, 1027
CaiI CAGNNNCTG 2 cut(s) 158, 821
CfoI GCGC 1 cut(s) 196
Cfr13I GGNCC 3 cut(s) 293, 949, 962
Csp6I GTAC 6 cut(s) 56, 97, 238, 373, 698, 886
CviAII CATG 6 cut(s) 252, 314, 442, 868, 918, 954
CviQI GTAC 6 cut(s) 56, 97, 238, 373, 698, 886
DdeI CTNAG 6 cut(s) 60, 231, 558, 624, 633, 905
DpnI GATC 1 cut(s) 456
DpnII GATC 1 cut(s) 454
DraI TTTAAA 1 cut(s) 753
EaeI YGGCCR 1 cut(s) 1027
Eco130I CCWWGG 4 cut(s) 313, 517, 953, 969
Eco47I GGWCC 2 cut(s) 949, 962
Eco57I CTGAAG 3 cut(s) 12, 695, 1122
EcoNI CCTNNNNNAGG 1 cut(s) 201
EcoO109I RGGNCCY 2 cut(s) 293, 962
EcoRI GAATTC 1 cut(s) 586
EcoT14I CCWWGG 4 cut(s) 313, 517, 953, 969
ErhI CCWWGG 4 cut(s) 313, 517, 953, 969
FaeI CATG 6 cut(s) 255, 317, 445, 871, 921, 957
FatI CATG 6 cut(s) 251, 313, 441, 867, 917, 953
FauI CCCGC 1 cut(s) 739
Fnu4HI GCNGC 5 cut(s) 153, 156, 543, 728, 819
FokI GGATG 1 cut(s) 797
Fsp4HI GCNGC 5 cut(s) 153, 156, 543, 728, 819
FspBI CTAG 1 cut(s) 893
GlaI GCGC 1 cut(s) 195
GluI GCNGC 5 cut(s) 153, 156, 543, 728, 819
GsaI CCCAGC 2 cut(s) 162, 401
GsuI CTGGAG 1 cut(s) 361
HaeIII GGCC 3 cut(s) 88, 295, 1029
HapII CCGG 2 cut(s) 834, 862
HhaI GCGC 1 cut(s) 196
Hin1II CATG 6 cut(s) 255, 317, 445, 871, 921, 957
Hin6I GCGC 1 cut(s) 194
HinP1I GCGC 1 cut(s) 194
HinfI GANTC 3 cut(s) 743, 800, 981
HpaII CCGG 2 cut(s) 834, 862
HphI GGTGA 1 cut(s) 953
Hpy166II GTNNAC 6 cut(s) 72, 466, 620, 888, 1037, 1063
Hpy188I TCNGA 2 cut(s) 551, 634
Hpy188III TCNNGA 3 cut(s) 472, 688, 893
Hpy8I GTNNAC 6 cut(s) 72, 466, 620, 888, 1037, 1063
Hpy99I CGWCG 2 cut(s) 10, 13
HpyAV CCTTC 3 cut(s) 284, 469, 670
HpyCH4III ACNGT 4 cut(s) 499, 600, 646, 1034
HpyCH4IV ACGT 1 cut(s) 780
HpyCH4V TGCA 8 cut(s) 17, 77, 152, 283, 542, 578, 611, 1021
HpyF10VI GCNNNNNNNGC 2 cut(s) 539, 724
HpyF3I CTNAG 6 cut(s) 60, 231, 558, 624, 633, 905
HpySE526I ACGT 1 cut(s) 780
Hsp92II CATG 6 cut(s) 255, 317, 445, 871, 921, 957
HspAI GCGC 1 cut(s) 194
Kzo9I GATC 1 cut(s) 454
Lsp1109I GCAGC 5 cut(s) 142, 164, 554, 739, 830
LweI GCATC 1 cut(s) 427
MaeI CTAG 1 cut(s) 893
MaeII ACGT 1 cut(s) 780
MaeIII GTNAC 1 cut(s) 941
MalI GATC 1 cut(s) 456
MbiI CCGCTC 1 cut(s) 734
MboI GATC 1 cut(s) 454
MboII GAAGA 1 cut(s) 171
MlsI TGGCCA 1 cut(s) 1029
MluCI AATT 5 cut(s) 241, 267, 586, 1000, 1015
MluNI TGGCCA 1 cut(s) 1029
MmeI TCCRAC 4 cut(s) 487, 529, 550, 801
Mox20I TGGCCA 1 cut(s) 1029
MroXI GAANNNNTTC 1 cut(s) 68
MscI TGGCCA 1 cut(s) 1029
MseI TTAA 1 cut(s) 752
Msp20I TGGCCA 1 cut(s) 1029
MspA1I CMGCKG 2 cut(s) 155, 821
MspI CCGG 2 cut(s) 834, 862
MspR9I CCNGG 1 cut(s) 862
MwoI GCNNNNNNNGC 2 cut(s) 539, 724
NciI CCSGG 1 cut(s) 862
NcoI CCATGG 2 cut(s) 313, 953
NdeII GATC 1 cut(s) 454
NlaIII CATG 6 cut(s) 255, 317, 445, 871, 921, 957
NlaIV GGNNCC 1 cut(s) 951
NmuCI GTSAC 1 cut(s) 941
PdmI GAANNNNTTC 1 cut(s) 68
PfeI GAWTC 3 cut(s) 743, 800, 981
PflMI CCANNNNNTGG 1 cut(s) 447
PkrI GCNGC 5 cut(s) 154, 157, 544, 729, 820
PpuMI RGGWCCY 1 cut(s) 962
Psp5II RGGWCCY 1 cut(s) 962
PspFI CCCAGC 2 cut(s) 158, 397
PspN4I GGNNCC 1 cut(s) 951
PspPI GGNCC 3 cut(s) 293, 949, 962
PspPPI RGGWCCY 1 cut(s) 962
PstNI CAGNNNCTG 2 cut(s) 158, 821
PvuII CAGCTG 2 cut(s) 155, 821
RsaI GTAC 6 cut(s) 57, 98, 239, 374, 699, 887
RsaNI GTAC 6 cut(s) 56, 97, 238, 373, 698, 886
SaqAI TTAA 1 cut(s) 752
SatI GCNGC 5 cut(s) 153, 156, 543, 728, 819
Sau3AI GATC 1 cut(s) 454
Sau96I GGNCC 3 cut(s) 293, 949, 962
ScaI AGTACT 1 cut(s) 699
ScrFI CCNGG 1 cut(s) 862
SfaNI GCATC 1 cut(s) 427
SfcI CTRYAG 1 cut(s) 387
SinI GGWCC 2 cut(s) 949, 962
Sse9I AATT 5 cut(s) 241, 267, 586, 1000, 1015
SsiI CCGC 2 cut(s) 531, 732
SspMI CTAG 1 cut(s) 893
StyD4I CCNGG 1 cut(s) 860
StyI CCWWGG 4 cut(s) 313, 517, 953, 969
TaaI ACNGT 4 cut(s) 499, 600, 646, 1034
TaiI ACGT 1 cut(s) 783
TaqI TCGA 2 cut(s) 23, 1004
TasI AATT 5 cut(s) 241, 267, 586, 1000, 1015
TatI WGTACW 3 cut(s) 372, 697, 885
TfiI GAWTC 3 cut(s) 743, 800, 981
Tru1I TTAA 1 cut(s) 752
Tru9I TTAA 1 cut(s) 752
TscAI CASTG 7 cut(s) 36, 79, 344, 429, 504, 678, 1037
TseFI GTSAC 1 cut(s) 941
TseI GCWGC 5 cut(s) 152, 155, 542, 727, 818
Tsp45I GTSAC 1 cut(s) 941
TspDTI ATGAA 5 cut(s) 134, 171, 260, 856, 1059
TspGWI ACGGA 1 cut(s) 43
TspRI CASTG 7 cut(s) 36, 79, 344, 429, 504, 678, 1037
Van91I CCANNNNNTGG 1 cut(s) 447
VpaK11BI GGWCC 2 cut(s) 949, 962
XagI CCTNNNNNAGG 1 cut(s) 201
XapI RAATTY 4 cut(s) 267, 586, 1000, 1015
XbaI TCTAGA 1 cut(s) 892
XmnI GAANNNNTTC 1 cut(s) 68
XspI CTAG 1 cut(s) 893
ZrmI AGTACT 1 cut(s) 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.