FvH4_1g13010

Transmembrane protein 53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014260
Physical Location & Seq
Forward (+)
7172349 .. 7174191
1843 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g13010.t1

Sequence Viewer

Length: 1176 bp
ATGGAAACAACCCCACTGAGACTTCTCCGCCTCAAAACGACGCCGTCCGCTCTCTTTCTCTCTCCTCCCAAATTCCACCGACCTCAAACAGCACTCGCATTTCCCAATCTCCGGCGAAGCGACGCCGTAAAGCGAACCCCTTTCGCTCTCTCTCACTCCTCCTTCTCCCCAAACCCTAACCCCTTCAGATTCACCTCCTCCGTTTCCCAATCGCCGTTTCCGATCATCAATTCCAACCTCAACTACTCTCTCTATCATCAAAACGACGCCGGATTCAGATGGAGCCGAGCTTCCCGGAGCGGCGTTGACGGAAATGTGGGCCTTTTGGGGCCGGAAAAGCCGGAAGTGGCGGTGGTGCTGCTGGGGTGGCTGGGCGGGCAGAGCAAGCACCTGAAGAGGTACGTGGAGTGGTACAACTCCCGAGGCGTTGACGCGGTGACGTTTGTGGTGGACGTGAAGGAGGTGTTGGGGTTTGATTTGGGCCACCGGGTCGAGAAACGGGTCGCGGAGCTGGCCCGTGAGGTGGCTTCGTGGGTTGAGGGCGGAGGGGAACGGTGGTTGGTGTTTCACACTTTCAGTAATACGGGTTGGTTTGTATATGGTGCATTGCTTGAGATTTGGGAGGATAGGCCGGACTTGATCGAGAAGATTAGAGGGTGTGTTGTTGATTCAGGAGCTGGTGCCCCTTTTGATCCTAAGGTGTGGGCAGCGGGATTCTCTACTGCAATTTTGAAGAAAAACAGCTCTTTAGCAAAACCTGCGGTTGAGACTAAAGAGTTAAATGCCTCTGGAGGTGCAGCGAGCTTGTCAAAGGTGCAAGAAGAGGAACCTCCAGCAATTGAAACCATGCTTCTTATGGTATTAGAGAAGCTGTTCTCATTCATTCTAAAGTTGCCTGATGTGGATAAGAGGTTAACCAAGGTAGTTTCAGTCCTCTCAACAAACCCTCCCCACTGTCCACAGCTTTACCTTTACAGCACGGGTGACAAAGTCGTCCCACACCAATCCATCGAGTCCTTCATCGCAGAGCAAAGGAAAATAGGTAGAACAGTGAAGTCCTTCAACTTCGGCACATCCCCTCACGTCGACCATTACAGGACTTTCCCCAACATATACTCTCAAGAGCTCAACCATTTCTTGAAAGAATGCTTGGCTACACCAGTTAAGCAGACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

392

Amino Acids

43.62

Weight (kDa)

9.26

Isoelectric Point (pI)

50.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 117 - 380 1.1e-59 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18245
fragaria_vesca FvH4_1g13010
malus_domestica MD02G1144600.v1.1 MD15G1258300.v1.1
prunus_persica Prupe.7G157000_v2.0.a1 Prupe.7G157000_v2.0.a1
pyrus_communis pycom02g11450 pycom15g22610
rosa_chinensis RchiOBHm_Chr2g0101161
rosa_laevigata RLG00000017051
rosa_roxburghii Rroxscaffold_2G00141530
rosa_rugosa Rorug02G0100600
rosa_samantha Rh2AG144000 Rh2BG149000 Rh2CG150300 Rh2DG149500
rosa_wichuraiana Rw2G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 992
Acc36I ACCTGC 1 cut(s) 766
AccB1I GGYRCC 1 cut(s) 680
AccBSI CCGCTC 2 cut(s) 50, 300
AccI GTMKAC 1 cut(s) 1086
AccII CGCG 2 cut(s) 434, 506
AclWI GGATC 1 cut(s) 686
AcsI RAATTY 1 cut(s) 71
AcuI CTGAAG 2 cut(s) 169, 413
AcyI GRCGYC 3 cut(s) 41, 123, 267
AfaI GTAC 2 cut(s) 401, 413
AfiI CCNNNNNNNGG 2 cut(s) 111, 523
AgsI TTSAA 4 cut(s) 733, 842, 1063, 1141
AjiI CACGTC 2 cut(s) 454, 1084
AjuI GAANNNNNNNTTGG 6 cut(s) 201, 233, 449, 481, 1133, 1165
AluBI AGCT 8 cut(s) 290, 511, 677, 744, 804, 871, 964, 1126
AluI AGCT 8 cut(s) 290, 511, 677, 744, 804, 871, 964, 1126
Alw21I GWGCWC 1 cut(s) 1128
Alw26I GTCTC 2 cut(s) 13, 761
AlwI GGATC 1 cut(s) 686
AlwNI CAGNNNCTG 1 cut(s) 677
Ama87I CYCGRG 1 cut(s) 420
AoxI GGCC 5 cut(s) 319, 329, 481, 513, 629
ApeKI GCWGC 3 cut(s) 358, 707, 797
ApoI RAATTY 1 cut(s) 71
Asp700I GAANNNNTTC 2 cut(s) 872, 1058
AspS9I GGNCC 4 cut(s) 319, 329, 481, 514
AsuC2I CCSGG 2 cut(s) 295, 488
AsuHPI GGTGA 3 cut(s) 184, 448, 995
AvaI CYCGRG 1 cut(s) 420
AxyI CCTNAGG 1 cut(s) 696
BaeGI GKGCMC 1 cut(s) 685
BanI GGYRCC 1 cut(s) 680
BanII GRGCYC 1 cut(s) 1128
Bbv12I GWGCWC 1 cut(s) 1128
BbvI GCAGC 3 cut(s) 345, 719, 809
BccI CCATC 2 cut(s) 273, 1016
BceAI ACGGC 3 cut(s) 28, 110, 199
BcnI CCSGG 2 cut(s) 295, 488
BcoDI GTCTC 2 cut(s) 13, 761
BfuAI ACCTGC 1 cut(s) 766
BisI GCNGC 4 cut(s) 301, 359, 708, 798
BlsI GCNGC 4 cut(s) 302, 360, 709, 799
Bme1390I CCNGG 2 cut(s) 295, 488
BmeT110I CYCGRG 1 cut(s) 420
BmgBI CACGTC 2 cut(s) 454, 1084
BmgT120I GGNCC 4 cut(s) 319, 329, 481, 514
BmiI GGNNCC 4 cut(s) 284, 330, 682, 828
BmrFI CCNGG 2 cut(s) 295, 488
BpmI CTGGAG 2 cut(s) 810, 816
BpuEI CTTGAG 2 cut(s) 632, 1104
BpuMI CCSGG 2 cut(s) 295, 488
BsaAI YACGTR 1 cut(s) 403
BsaHI GRCGYC 3 cut(s) 41, 123, 267
BsaJI CCNNGG 2 cut(s) 421, 918
BsaXI ACNNNNNCTCC 2 cut(s) 931, 961
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 523
Bse1I ACTGG 1 cut(s) 1160
Bse21I CCTNAGG 1 cut(s) 696
Bse3DI GCAATG 1 cut(s) 605
BseDI CCNNGG 2 cut(s) 421, 918
BseGI GGATG 1 cut(s) 1073
BseLI CCNNNNNNNGG 2 cut(s) 111, 523
BseMI GCAATG 1 cut(s) 605
BseMII CTCAG 1 cut(s) 8
BseNI ACTGG 1 cut(s) 1160
BseRI GAGGAG 3 cut(s) 54, 148, 187
BseSI GKGCMC 1 cut(s) 685
BseXI GCAGC 3 cut(s) 345, 719, 809
BseYI CCCAGC 2 cut(s) 361, 370
BsgI GTGCAG 1 cut(s) 816
Bsh1236I CGCG 2 cut(s) 434, 506
BshFI GGCC 5 cut(s) 321, 331, 483, 515, 631
BshNI GGYRCC 1 cut(s) 680
BsiHKAI GWGCWC 1 cut(s) 1128
BsiHKCI CYCGRG 1 cut(s) 420
BsiSI CCGG 7 cut(s) 112, 270, 295, 332, 341, 487, 632
BslFI GGGAC 1 cut(s) 980
BslI CCNNNNNNNGG 2 cut(s) 111, 523
BsmAI GTCTC 2 cut(s) 13, 761
BsmFI GGGAC 1 cut(s) 980
BsmI GAATGC 1 cut(s) 1151
BsnI GGCC 5 cut(s) 321, 331, 483, 515, 631
BsoBI CYCGRG 1 cut(s) 420
Bsp1286I GDGCHC 2 cut(s) 685, 1128
Bsp143I GATC 3 cut(s) 222, 639, 691
BspANI GGCC 5 cut(s) 321, 331, 483, 515, 631
BspCNI CTCAG 1 cut(s) 9
BspFNI CGCG 2 cut(s) 434, 506
BspLI GGNNCC 4 cut(s) 284, 330, 682, 828
BspMI ACCTGC 1 cut(s) 766
BspPI GGATC 1 cut(s) 686
BspT107I GGYRCC 1 cut(s) 680
BsrBI CCGCTC 2 cut(s) 50, 300
BsrDI GCAATG 1 cut(s) 605
BsrI ACTGG 1 cut(s) 1160
BssECI CCNNGG 2 cut(s) 421, 918
BssMI GATC 3 cut(s) 222, 639, 691
BssNI GRCGYC 3 cut(s) 41, 123, 267
BssT1I CCWWGG 1 cut(s) 918
Bst4CI ACNGT 3 cut(s) 555, 956, 1051
Bst6I CTCTTC 2 cut(s) 389, 816
BstACI GRCGYC 3 cut(s) 41, 123, 267
BstAPI GCANNNNNTGC 1 cut(s) 758
BstBAI YACGTR 1 cut(s) 403
BstC8I GCNNGC 4 cut(s) 377, 386, 513, 802
BstDEI CTNAG 2 cut(s) 17, 696
BstF5I GGATG 1 cut(s) 1073
BstFNI CGCG 2 cut(s) 434, 506
BstKTI GATC 3 cut(s) 225, 642, 694
BstMAI GTCTC 2 cut(s) 13, 761
BstMBI GATC 3 cut(s) 222, 639, 691
BstMWI GCNNNNNNNGC 7 cut(s) 337, 367, 376, 381, 385, 512, 758
BstSCI CCNGG 2 cut(s) 293, 486
BstSLI GKGCMC 1 cut(s) 685
BstUI CGCG 2 cut(s) 434, 506
BstV1I GCAGC 3 cut(s) 345, 719, 809
Bsu36I CCTNAGG 1 cut(s) 696
BsuRI GGCC 5 cut(s) 321, 331, 483, 515, 631
BtgZI GCGATG 1 cut(s) 1006
BtrI CACGTC 2 cut(s) 454, 1084
BtsCI GGATG 1 cut(s) 1073
BtsIMutI CAGTG 3 cut(s) 14, 952, 1056
BveI ACCTGC 1 cut(s) 766
Cac8I GCNNGC 4 cut(s) 377, 386, 513, 802
CaiI CAGNNNCTG 1 cut(s) 677
Cfr13I GGNCC 4 cut(s) 319, 329, 481, 514
CseI GACGC 4 cut(s) 49, 131, 275, 440
Csp6I GTAC 2 cut(s) 400, 412
CviAII CATG 1 cut(s) 847
CviQI GTAC 2 cut(s) 400, 412
DdeI CTNAG 2 cut(s) 17, 696
DpnI GATC 3 cut(s) 224, 641, 693
DpnII GATC 3 cut(s) 222, 639, 691
DrdI GACNNNNNNGTC 1 cut(s) 992
DseDI GACNNNNNNGTC 1 cut(s) 992
Eam1104I CTCTTC 2 cut(s) 389, 816
EarI CTCTTC 2 cut(s) 389, 816
EciI GGCGGA 2 cut(s) 17, 558
Ecl136II GAGCTC 1 cut(s) 1126
Eco130I CCWWGG 1 cut(s) 918
Eco24I GRGCYC 1 cut(s) 1128
Eco53kI GAGCTC 1 cut(s) 1126
Eco57I CTGAAG 2 cut(s) 169, 413
Eco81I CCTNAGG 1 cut(s) 696
Eco88I CYCGRG 1 cut(s) 420
EcoICRI GAGCTC 1 cut(s) 1126
EcoT14I CCWWGG 1 cut(s) 918
EcoT38I GRGCYC 1 cut(s) 1128
ErhI CCWWGG 1 cut(s) 918
FaeI CATG 1 cut(s) 850
FaiI YATR 6 cut(s) 598, 600, 848, 857, 1112, 1114
FaqI GGGAC 1 cut(s) 980
FatI CATG 1 cut(s) 846
FauI CCCGC 2 cut(s) 368, 703
FblI GTMKAC 1 cut(s) 1086
Fnu4HI GCNGC 4 cut(s) 301, 359, 708, 798
FokI GGATG 1 cut(s) 1060
FriOI GRGCYC 1 cut(s) 1128
Fsp4HI GCNGC 4 cut(s) 301, 359, 708, 798
GluI GCNGC 4 cut(s) 301, 359, 708, 798
GsaI CCCAGC 2 cut(s) 365, 374
GsuI CTGGAG 2 cut(s) 810, 816
HaeIII GGCC 5 cut(s) 321, 331, 483, 515, 631
HapII CCGG 7 cut(s) 112, 270, 295, 332, 341, 487, 632
HgaI GACGC 4 cut(s) 49, 131, 275, 440
Hin1I GRCGYC 3 cut(s) 41, 123, 267
Hin1II CATG 1 cut(s) 850
HincII GTYRAC 4 cut(s) 307, 430, 915, 1087
HindII GTYRAC 4 cut(s) 307, 430, 915, 1087
HinfI GANTC 5 cut(s) 189, 273, 668, 714, 1013
HpaI GTTAAC 1 cut(s) 915
HpaII CCGG 7 cut(s) 112, 270, 295, 332, 341, 487, 632
HphI GGTGA 3 cut(s) 184, 448, 995
Hpy166II GTNNAC 6 cut(s) 307, 430, 451, 915, 959, 1087
Hpy188I TCNGA 3 cut(s) 188, 222, 278
Hpy188III TCNNGA 7 cut(s) 420, 493, 643, 672, 789, 1121, 1138
Hpy8I GTNNAC 6 cut(s) 307, 430, 451, 915, 959, 1087
Hpy99I CGWCG 4 cut(s) 43, 125, 269, 1088
HpyAV CCTTC 5 cut(s) 172, 193, 451, 1027, 1069
HpyCH4III ACNGT 3 cut(s) 555, 956, 1051
HpyCH4IV ACGT 5 cut(s) 402, 440, 453, 1083, 1172
HpyCH4V TGCA 4 cut(s) 605, 725, 797, 817
HpyF10VI GCNNNNNNNGC 7 cut(s) 337, 367, 376, 381, 385, 512, 758
HpyF3I CTNAG 2 cut(s) 17, 696
HpySE526I ACGT 5 cut(s) 402, 440, 453, 1083, 1172
Hsp92I GRCGYC 3 cut(s) 41, 123, 267
Hsp92II CATG 1 cut(s) 850
KspAI GTTAAC 1 cut(s) 915
Kzo9I GATC 3 cut(s) 222, 639, 691
LmnI GCTCC 4 cut(s) 282, 297, 508, 674
Lsp1109I GCAGC 3 cut(s) 345, 719, 809
MaeII ACGT 5 cut(s) 402, 440, 453, 1083, 1172
MaeIII GTNAC 2 cut(s) 436, 983
MalI GATC 3 cut(s) 224, 641, 693
MbiI CCGCTC 2 cut(s) 50, 300
MboI GATC 3 cut(s) 222, 639, 691
MboII GAAGA 4 cut(s) 406, 658, 745, 833
MfeI CAATTG 1 cut(s) 837
MhlI GDGCHC 2 cut(s) 685, 1128
MluCI AATT 4 cut(s) 71, 229, 726, 837
MlyI GAGTC 1 cut(s) 1022
MmeI TCCRAC 1 cut(s) 258
MroXI GAANNNNTTC 2 cut(s) 872, 1058
MseI TTAA 3 cut(s) 779, 914, 1164
MspA1I CMGCKG 1 cut(s) 710
MspI CCGG 7 cut(s) 112, 270, 295, 332, 341, 487, 632
MspR9I CCNGG 2 cut(s) 295, 488
MunI CAATTG 1 cut(s) 837
Mva1269I GAATGC 1 cut(s) 1151
MvnI CGCG 2 cut(s) 434, 506
MwoI GCNNNNNNNGC 7 cut(s) 337, 367, 376, 381, 385, 512, 758
NciI CCSGG 2 cut(s) 295, 488
NdeII GATC 3 cut(s) 222, 639, 691
NlaIII CATG 1 cut(s) 850
NlaIV GGNNCC 4 cut(s) 284, 330, 682, 828
NmeAIII GCCGAG 1 cut(s) 311
NmuCI GTSAC 2 cut(s) 436, 983
PcsI WCGNNNNNNNCGW 1 cut(s) 218
PctI GAATGC 1 cut(s) 1151
PdmI GAANNNNTTC 2 cut(s) 872, 1058
PfeI GAWTC 4 cut(s) 189, 273, 668, 714
PflFI GACNNNGTC 2 cut(s) 43, 989
PfoI TCCNGGA 1 cut(s) 293
PkrI GCNGC 4 cut(s) 302, 360, 709, 799
PleI GAGTC 1 cut(s) 1021
PpsI GAGTC 1 cut(s) 1021
Ppu21I YACGTR 1 cut(s) 403
Psp124BI GAGCTC 1 cut(s) 1128
PspFI CCCAGC 2 cut(s) 361, 370
PspN4I GGNNCC 4 cut(s) 284, 330, 682, 828
PspPI GGNCC 4 cut(s) 319, 329, 481, 514
PstNI CAGNNNCTG 1 cut(s) 677
PsyI GACNNNGTC 2 cut(s) 43, 989
RsaI GTAC 2 cut(s) 401, 413
RsaNI GTAC 2 cut(s) 400, 412
SacI GAGCTC 1 cut(s) 1128
SalI GTCGAC 1 cut(s) 1085
SaqAI TTAA 3 cut(s) 779, 914, 1164
SatI GCNGC 4 cut(s) 301, 359, 708, 798
Sau3AI GATC 3 cut(s) 222, 639, 691
Sau96I GGNCC 4 cut(s) 319, 329, 481, 514
SchI GAGTC 1 cut(s) 1022
ScrFI CCNGG 2 cut(s) 295, 488
SduI GDGCHC 2 cut(s) 685, 1128
SmlI CTYRAG 2 cut(s) 611, 1119
SmoI CTYRAG 2 cut(s) 611, 1119
Sse9I AATT 4 cut(s) 71, 229, 726, 837
SstI GAGCTC 1 cut(s) 1128
StyD4I CCNGG 2 cut(s) 293, 486
StyI CCWWGG 1 cut(s) 918
TaaI ACNGT 3 cut(s) 555, 956, 1051
TaiI ACGT 5 cut(s) 405, 443, 456, 1086, 1175
TaqI TCGA 4 cut(s) 492, 642, 1011, 1086
TasI AATT 4 cut(s) 71, 229, 726, 837
TauI GCSGC 1 cut(s) 303
TfiI GAWTC 4 cut(s) 189, 273, 668, 714
Tru1I TTAA 3 cut(s) 779, 914, 1164
Tru9I TTAA 3 cut(s) 779, 914, 1164
TscAI CASTG 3 cut(s) 21, 959, 1056
TseFI GTSAC 2 cut(s) 436, 983
TseI GCWGC 3 cut(s) 358, 707, 797
Tsp45I GTSAC 2 cut(s) 436, 983
TspDTI ATGAA 2 cut(s) 871, 1009
TspGWI ACGGA 2 cut(s) 190, 324
TspRI CASTG 3 cut(s) 21, 959, 1056
Tth111I GACNNNGTC 2 cut(s) 43, 989
XapI RAATTY 1 cut(s) 71
XcmI CCANNNNNNNNNTGG 1 cut(s) 853
XmiI GTMKAC 1 cut(s) 1086
XmnI GAANNNNTTC 2 cut(s) 872, 1058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.