RchiOBHm_Chr2g0101161

Transmembrane protein 53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014260
Physical Location & Seq
Forward (+)
12857472 .. 12859607
2136 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47578

Sequence Viewer

Length: 1185 bp
ATGGAGACTCCACTGAGAATCCTCAGCCTCAACCGCCACCTCAAAACGACGCCGTCCGCTCTTTTCTTCTCCCCCAAATTTCACCCACCTCAAAAACCCTCACTTGCATTTCCCAATTATTTCCGGCTAAACGACGCCGTAAAGAGAACCCCTTTTGCTCTTTCTCACTCCTCCTCTTCCTGGAATCCAAACCCCTTCAAATTCAACCCCTCAATTTCCCAATCTCCATTTCCGATCACCAATTTCAACCACTCCCTGTCGTACCAAAACGACGCCGGTTTCAGATGGAACCGAGCGGTCCACAGCGGCATTGACGGAAATGTGGGATCTTTGGGGGCCCAAAAGCGGGAAGTGACGGTGGTGTTGCTGGGCTGGCTTGGGGGTCAGACCAAGCACCTGAAGAGGTACGTGGAGTGGTACAATTCCCGAGGCTTTCACGCTGTGACGTTTGTTGTGGACGTCAAGGAGGTGCTCTGGTTTGATTTGGGCTACCGGGTTGAGAAGCGGGTCGCGGAGCTGGCCCATGAGCTGGCTTCGTGGGTTGAGGGCGGCGGAGAACGGTGTTTGGTGTTTCACACTTTTAGCAATACGGGTTGGTTTGTATATGGTGCAATACTTGAAATTTGGCTGGATAGACCAGACTTGATCAAGAAGATTAAAGGGTGTATTGTTGATTCAGGAGCTGGAGCCCCTTTTGATCCTAAGGTGTGGGCAGCAGGATTCTCTACTGCGATTATGAAGAAACGCAGCTCTTTAGCAAAACCTGCGGTTGAGACTAAAGAATTAAATGCCTCTGGAGGTGGTGCAGCGAGCTTGTCAAAGGTGCAAGAAGAGGAACCTCCAGCAATTGAAACCATGCTTCTGTTGGTATTAGAGAAGCTGTTCTCACTCATTCTGAAGTTGCCTGATGTGGATAAGAGGTTAACCAAGGTAGTTTCTGTCCTCTCGACAAACCCTCCTCATTGTCCACAGCTTTACCTGTACAGTACGGGTGATAAAGTCGTCCCACACCAGTCCATCGAATCCTTCATCGCAGAGCAAAGGCAGATAGGTAGACCCGTGAAGTCCTTCAATTTCGGCACATCTCCTCACGTCGACCATTACAGGACTTTCCCCAACATATACTCTCAGGAACTTAACAATTTCTTGAAAGAATGCTTGGCTACAACAGTTAAGCAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

394

Amino Acids

44.22

Weight (kDa)

9.38

Isoelectric Point (pI)

43.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 119 - 383 1.2e-62 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18245
fragaria_vesca FvH4_1g13010
malus_domestica MD02G1144600.v1.1 MD15G1258300.v1.1
prunus_persica Prupe.7G157000_v2.0.a1 Prupe.7G157000_v2.0.a1
pyrus_communis pycom02g11450 pycom15g22610
rosa_chinensis RchiOBHm_Chr2g0101161
rosa_laevigata RLG00000017051
rosa_roxburghii Rroxscaffold_2G00141530
rosa_rugosa Rorug02G0100600
rosa_samantha Rh2AG144000 Rh2BG149000 Rh2CG150300 Rh2DG149500
rosa_wichuraiana Rw2G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 462
Acc36I ACCTGC 1 cut(s) 772
AccB7I CCANNNNNTGG 1 cut(s) 529
AccBSI CCGCTC 2 cut(s) 59, 296
AccI GTMKAC 2 cut(s) 1054, 1095
AccII CGCG 1 cut(s) 512
AclWI GGATC 2 cut(s) 334, 692
AcsI RAATTY 3 cut(s) 77, 200, 621
AcuI CTGAAG 2 cut(s) 419, 917
AcyI GRCGYC 4 cut(s) 50, 135, 273, 459
AdeI CACNNNGTG 1 cut(s) 442
AfaI GTAC 5 cut(s) 263, 407, 419, 983, 988
AfiI CCNNNNNNNGG 4 cut(s) 180, 345, 346, 529
AgsI TTSAA 7 cut(s) 199, 205, 247, 620, 851, 1072, 1150
AjiI CACGTC 1 cut(s) 1093
AjnI CCWGG 1 cut(s) 179
AjuI GAANNNNNNNTTGG 4 cut(s) 213, 245, 1142, 1174
AluBI AGCT 7 cut(s) 517, 529, 683, 750, 813, 880, 973
AluI AGCT 7 cut(s) 517, 529, 683, 750, 813, 880, 973
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 1 cut(s) 767
AlwI GGATC 2 cut(s) 334, 692
AlwNI CAGNNNCTG 1 cut(s) 683
Ama87I CYCGRG 1 cut(s) 426
AoxI GGCC 2 cut(s) 336, 519
ApaI GGGCCC 1 cut(s) 340
ApeKI GCWGC 3 cut(s) 713, 747, 806
ApoI RAATTY 3 cut(s) 77, 200, 621
Asp700I GAANNNNTTC 2 cut(s) 881, 1067
AspS9I GGNCC 4 cut(s) 298, 336, 337, 520
AsuC2I CCSGG 1 cut(s) 494
AsuHPI GGTGA 3 cut(s) 74, 229, 1004
AvaI CYCGRG 1 cut(s) 426
AvaII GGWCC 1 cut(s) 298
AxyI CCTNAGG 1 cut(s) 702
BaeGI GKGCMC 1 cut(s) 340
BanII GRGCYC 2 cut(s) 340, 691
Bbv12I GWGCWC 1 cut(s) 474
BbvCI CCTCAGC 1 cut(s) 23
BbvI GCAGC 3 cut(s) 725, 759, 818
BccI CCATC 2 cut(s) 279, 1025
BceAI ACGGC 2 cut(s) 37, 122
BciT130I CCWGG 1 cut(s) 181
BclI TGATCA 1 cut(s) 645
BcnI CCSGG 1 cut(s) 494
BcoDI GTCTC 1 cut(s) 767
BfuAI ACCTGC 1 cut(s) 772
BisI GCNGC 5 cut(s) 307, 550, 714, 748, 807
BlsI GCNGC 5 cut(s) 308, 551, 715, 749, 808
Bme1390I CCNGG 2 cut(s) 181, 494
Bme18I GGWCC 1 cut(s) 298
BmeT110I CYCGRG 1 cut(s) 426
BmgBI CACGTC 1 cut(s) 1093
BmgT120I GGNCC 4 cut(s) 298, 336, 337, 520
BmiI GGNNCC 5 cut(s) 290, 337, 338, 688, 837
BmrFI CCNGG 2 cut(s) 181, 494
BpmI CTGGAG 3 cut(s) 705, 816, 825
Bpu10I CCTNAGC 1 cut(s) 23
BpuMI CCSGG 1 cut(s) 494
BsaAI YACGTR 1 cut(s) 409
BsaHI GRCGYC 4 cut(s) 50, 135, 273, 459
BsaJI CCNNGG 2 cut(s) 427, 927
BsaXI ACNNNNNCTCC 2 cut(s) 940, 970
Bsc4I CCNNNNNNNGG 4 cut(s) 180, 345, 346, 529
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 1 cut(s) 1012
Bse21I CCTNAGG 1 cut(s) 702
BseBI CCWGG 1 cut(s) 181
BseDI CCNNGG 2 cut(s) 427, 927
BseLI CCNNNNNNNGG 4 cut(s) 180, 345, 346, 529
BseMII CTCAG 3 cut(s) 5, 37, 1142
BseNI ACTGG 1 cut(s) 1012
BseRI GAGGAG 4 cut(s) 160, 163, 948, 1077
BseSI GKGCMC 1 cut(s) 340
BseXI GCAGC 3 cut(s) 725, 759, 818
BseYI CCCAGC 1 cut(s) 367
BsgI GTGCAG 1 cut(s) 825
Bsh1236I CGCG 1 cut(s) 512
BshFI GGCC 2 cut(s) 338, 521
BsiHKAI GWGCWC 1 cut(s) 474
BsiHKCI CYCGRG 1 cut(s) 426
BsiSI CCGG 3 cut(s) 124, 276, 493
BslFI GGGAC 1 cut(s) 989
BslI CCNNNNNNNGG 4 cut(s) 180, 345, 346, 529
BsmAI GTCTC 1 cut(s) 767
BsmFI GGGAC 1 cut(s) 989
BsmI GAATGC 1 cut(s) 1160
BsnI GGCC 2 cut(s) 338, 521
BsoBI CYCGRG 1 cut(s) 426
Bsp120I GGGCCC 1 cut(s) 336
Bsp1286I GDGCHC 3 cut(s) 340, 474, 691
Bsp1407I TGTACA 1 cut(s) 981
Bsp143I GATC 4 cut(s) 234, 326, 645, 697
BspANI GGCC 2 cut(s) 338, 521
BspCNI CTCAG 3 cut(s) 6, 36, 1141
BspFNI CGCG 1 cut(s) 512
BspLI GGNNCC 5 cut(s) 290, 337, 338, 688, 837
BspMI ACCTGC 1 cut(s) 772
BspPI GGATC 2 cut(s) 334, 692
BsrBI CCGCTC 2 cut(s) 59, 296
BsrFI RCCGGY 1 cut(s) 275
BsrGI TGTACA 1 cut(s) 981
BsrI ACTGG 1 cut(s) 1012
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 2 cut(s) 427, 927
BssMI GATC 4 cut(s) 234, 326, 645, 697
BssNI GRCGYC 4 cut(s) 50, 135, 273, 459
BssT1I CCWWGG 1 cut(s) 927
Bst2UI CCWGG 1 cut(s) 181
Bst4CI ACNGT 4 cut(s) 358, 561, 986, 1171
Bst6I CTCTTC 3 cut(s) 181, 395, 825
BstACI GRCGYC 4 cut(s) 50, 135, 273, 459
BstAPI GCANNNNNTGC 1 cut(s) 764
BstAUI TGTACA 1 cut(s) 981
BstBAI YACGTR 1 cut(s) 409
BstC8I GCNNGC 4 cut(s) 374, 519, 531, 811
BstDEI CTNAG 4 cut(s) 14, 23, 702, 1128
BstFNI CGCG 1 cut(s) 512
BstKTI GATC 4 cut(s) 237, 329, 648, 700
BstMAI GTCTC 1 cut(s) 767
BstMBI GATC 4 cut(s) 234, 326, 645, 697
BstMWI GCNNNNNNNGC 4 cut(s) 33, 373, 518, 764
BstNI CCWGG 1 cut(s) 181
BstSCI CCNGG 2 cut(s) 179, 492
BstSLI GKGCMC 1 cut(s) 340
BstUI CGCG 1 cut(s) 512
BstV1I GCAGC 3 cut(s) 725, 759, 818
BstX2I RGATCY 1 cut(s) 326
BstYI RGATCY 1 cut(s) 326
Bsu36I CCTNAGG 1 cut(s) 702
BsuRI GGCC 2 cut(s) 338, 521
BtgZI GCGATG 1 cut(s) 1015
BtrI CACGTC 1 cut(s) 1093
BtsIMutI CAGTG 1 cut(s) 11
BveI ACCTGC 1 cut(s) 772
Cac8I GCNNGC 4 cut(s) 374, 519, 531, 811
CaiI CAGNNNCTG 1 cut(s) 683
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 4 cut(s) 298, 336, 337, 520
CseI GACGC 3 cut(s) 58, 143, 281
Csp6I GTAC 5 cut(s) 262, 406, 418, 982, 987
CviAII CATG 2 cut(s) 524, 856
CviQI GTAC 5 cut(s) 262, 406, 418, 982, 987
DdeI CTNAG 4 cut(s) 14, 23, 702, 1128
DpnI GATC 4 cut(s) 236, 328, 647, 699
DpnII GATC 4 cut(s) 234, 326, 645, 697
DraIII CACNNNGTG 1 cut(s) 442
Eam1104I CTCTTC 3 cut(s) 181, 395, 825
EarI CTCTTC 3 cut(s) 181, 395, 825
EciI GGCGGA 1 cut(s) 567
Eco130I CCWWGG 1 cut(s) 927
Eco24I GRGCYC 2 cut(s) 340, 691
Eco47I GGWCC 1 cut(s) 298
Eco57I CTGAAG 2 cut(s) 419, 917
Eco81I CCTNAGG 1 cut(s) 702
Eco88I CYCGRG 1 cut(s) 426
EcoO109I RGGNCCY 1 cut(s) 336
EcoRII CCWGG 1 cut(s) 179
EcoT14I CCWWGG 1 cut(s) 927
EcoT38I GRGCYC 2 cut(s) 340, 691
ErhI CCWWGG 1 cut(s) 927
FaeI CATG 2 cut(s) 527, 859
FaiI YATR 7 cut(s) 525, 604, 606, 737, 857, 1121, 1123
FaqI GGGAC 1 cut(s) 989
FatI CATG 2 cut(s) 523, 855
FauI CCCGC 2 cut(s) 339, 498
FbaI TGATCA 1 cut(s) 645
FblI GTMKAC 2 cut(s) 1054, 1095
Fnu4HI GCNGC 5 cut(s) 307, 550, 714, 748, 807
FriOI GRGCYC 2 cut(s) 340, 691
Fsp4HI GCNGC 5 cut(s) 307, 550, 714, 748, 807
GluI GCNGC 5 cut(s) 307, 550, 714, 748, 807
GsaI CCCAGC 1 cut(s) 371
GsuI CTGGAG 3 cut(s) 705, 816, 825
HaeIII GGCC 2 cut(s) 338, 521
HapII CCGG 3 cut(s) 124, 276, 493
HgaI GACGC 3 cut(s) 58, 143, 281
Hin1I GRCGYC 4 cut(s) 50, 135, 273, 459
Hin1II CATG 2 cut(s) 527, 859
HincII GTYRAC 2 cut(s) 924, 1096
HindII GTYRAC 2 cut(s) 924, 1096
HinfI GANTC 6 cut(s) 7, 18, 184, 674, 720, 1022
HpaI GTTAAC 1 cut(s) 924
HpaII CCGG 3 cut(s) 124, 276, 493
HphI GGTGA 3 cut(s) 74, 229, 1004
Hpy166II GTNNAC 6 cut(s) 301, 457, 924, 968, 1055, 1096
Hpy188I TCNGA 4 cut(s) 234, 284, 387, 897
Hpy188III TCNNGA 7 cut(s) 426, 649, 678, 795, 946, 1130, 1147
Hpy8I GTNNAC 6 cut(s) 301, 457, 924, 968, 1055, 1096
Hpy99I CGWCG 4 cut(s) 52, 137, 275, 1097
HpyAV CCTTC 3 cut(s) 205, 1036, 1078
HpyCH4III ACNGT 4 cut(s) 358, 561, 986, 1171
HpyCH4IV ACGT 4 cut(s) 408, 446, 459, 1092
HpyCH4V TGCA 4 cut(s) 107, 611, 806, 826
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 373, 518, 764
HpyF3I CTNAG 4 cut(s) 14, 23, 702, 1128
HpySE526I ACGT 4 cut(s) 408, 446, 459, 1092
Hsp92I GRCGYC 4 cut(s) 50, 135, 273, 459
Hsp92II CATG 2 cut(s) 527, 859
Ksp22I TGATCA 1 cut(s) 645
KspAI GTTAAC 1 cut(s) 924
Kzo9I GATC 4 cut(s) 234, 326, 645, 697
LmnI GCTCC 3 cut(s) 514, 680, 686
Lsp1109I GCAGC 3 cut(s) 725, 759, 818
MaeII ACGT 4 cut(s) 408, 446, 459, 1092
MaeIII GTNAC 2 cut(s) 352, 442
MalI GATC 4 cut(s) 236, 328, 647, 699
MbiI CCGCTC 2 cut(s) 59, 296
MboI GATC 4 cut(s) 234, 326, 645, 697
MboII GAAGA 6 cut(s) 58, 168, 412, 664, 751, 842
MfeI CAATTG 1 cut(s) 846
MflI RGATCY 1 cut(s) 326
MhlI GDGCHC 3 cut(s) 340, 474, 691
MroXI GAANNNNTTC 2 cut(s) 881, 1067
MseI TTAA 5 cut(s) 657, 785, 923, 1137, 1173
MspA1I CMGCKG 1 cut(s) 306
MspI CCGG 3 cut(s) 124, 276, 493
MspR9I CCNGG 2 cut(s) 181, 494
MunI CAATTG 1 cut(s) 846
Mva1269I GAATGC 1 cut(s) 1160
MvaI CCWGG 1 cut(s) 181
MvnI CGCG 1 cut(s) 512
MwoI GCNNNNNNNGC 4 cut(s) 33, 373, 518, 764
NciI CCSGG 1 cut(s) 494
NdeII GATC 4 cut(s) 234, 326, 645, 697
NlaIII CATG 2 cut(s) 527, 859
NlaIV GGNNCC 5 cut(s) 290, 337, 338, 688, 837
NmuCI GTSAC 2 cut(s) 352, 442
PctI GAATGC 1 cut(s) 1160
PdmI GAANNNNTTC 2 cut(s) 881, 1067
PfeI GAWTC 5 cut(s) 18, 184, 674, 720, 1022
PflFI GACNNNGTC 1 cut(s) 52
PflMI CCANNNNNTGG 1 cut(s) 529
PfoI TCCNGGA 1 cut(s) 179
PkrI GCNGC 5 cut(s) 308, 551, 715, 749, 808
Ppu21I YACGTR 1 cut(s) 409
Psp6I CCWGG 1 cut(s) 179
PspFI CCCAGC 1 cut(s) 367
PspGI CCWGG 1 cut(s) 179
PspN4I GGNNCC 5 cut(s) 290, 337, 338, 688, 837
PspOMI GGGCCC 1 cut(s) 336
PspPI GGNCC 4 cut(s) 298, 336, 337, 520
PstNI CAGNNNCTG 1 cut(s) 683
PsuI RGATCY 1 cut(s) 326
PsyI GACNNNGTC 1 cut(s) 52
RsaI GTAC 5 cut(s) 263, 407, 419, 983, 988
RsaNI GTAC 5 cut(s) 262, 406, 418, 982, 987
SalI GTCGAC 1 cut(s) 1094
SaqAI TTAA 5 cut(s) 657, 785, 923, 1137, 1173
SatI GCNGC 5 cut(s) 307, 550, 714, 748, 807
Sau3AI GATC 4 cut(s) 234, 326, 645, 697
Sau96I GGNCC 4 cut(s) 298, 336, 337, 520
ScrFI CCNGG 2 cut(s) 181, 494
SduI GDGCHC 3 cut(s) 340, 474, 691
SinI GGWCC 1 cut(s) 298
StyD4I CCNGG 2 cut(s) 179, 492
StyI CCWWGG 1 cut(s) 927
TaaI ACNGT 4 cut(s) 358, 561, 986, 1171
TaiI ACGT 4 cut(s) 411, 449, 462, 1095
TaqI TCGA 3 cut(s) 947, 1020, 1095
TatI WGTACW 1 cut(s) 981
TauI GCSGC 2 cut(s) 309, 552
TfiI GAWTC 5 cut(s) 18, 184, 674, 720, 1022
Tru1I TTAA 5 cut(s) 657, 785, 923, 1137, 1173
Tru9I TTAA 5 cut(s) 657, 785, 923, 1137, 1173
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 2 cut(s) 352, 442
TseI GCWGC 3 cut(s) 713, 747, 806
Tsp45I GTSAC 2 cut(s) 352, 442
TspDTI ATGAA 2 cut(s) 752, 1018
TspGWI ACGGA 1 cut(s) 330
TspRI CASTG 1 cut(s) 18
Tth111I GACNNNGTC 1 cut(s) 52
Van91I CCANNNNNTGG 1 cut(s) 529
VpaK11BI GGWCC 1 cut(s) 298
XapI RAATTY 3 cut(s) 77, 200, 621
XcmI CCANNNNNNNNNTGG 1 cut(s) 862
XmiI GTMKAC 2 cut(s) 1054, 1095
XmnI GAANNNNTTC 2 cut(s) 881, 1067
ZraI GACGTC 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.