RLG00000017051

Transmembrane protein 53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014260
Physical Location & Seq
Forward (+)
12427788 .. 12429682
1895 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017051

Sequence Viewer

Length: 1191 bp
ATGGAGACTCCACTGAGAATCCTCAGCTTTAACCGCCACCTGAAAACGACGCCGTCCGCTCTTTTCTTCTCCCCCAAATTTCACCCACCTCAAAAACCCTCACTTGCATTTCCCAATTTCCGGCAAATCGACGCCGTAAAGAGAACCCCTTTTGCTCTTTCTCACTCCTCCTTCTATTCGAATCCAAACCCTTTCAAATTCAACCCCTCAATTTCCCAATCTGCATTGCCGATCACTAATTTCAACTACTCTCTGTCGAACCAAAACGACGCCGGTTTCAGATGGAACCGAGCGGTCCACAGCGGCATTGACGGAAATGTGGGACCTTTGGGGGCCCAGAAGCGGGAAGTGACGGTGGTGTTACTGGGCTGGCTTGGGGGTCAGACCAAGCACCTGAAGAGGTACGTGGAGTGGTACAATTCCCGAGGCTTTCACGCTGTGACGTTTGTTGTGGATGTGAAGGAGGTGCTCTGGTTTGATTTGGGCCACCGGGTTGAGAAGCGGGTCGCGGAGCTGGCCCATGAGCTGGCTTCGTGGGTCTCGGAGCGGGTTGAGCGTGGTGGAGAACGGTGTTTGGTGTTTCACACGTTTAGTAATACGGGTTGGTTTGTATATGGTGCAATACTTGAAATTTGGCTGGATAGACCAGACTTGATCAAGAGGATTAAAGGGTGTGTTGTTGATTCAGGAGCTGGAGCCCCTTTTGATCCTAAGGTGTGGGCAGCAGGATTCTCCACTGCGATTATGAAGAAACGCAGCTCTTTAGCAAAACCTGTGGTTGAGACTAAAGAATTAAATGCCTCTGGAGGTGCAGCGAGCTTGTCAAAGGTGCAAGAAGAGGAACCTCCAGCAATTGAAACCATGCTTCTGTTGGTATTAGAGAAGCTGTTCTCTCTCATTCTAAAGTTGCCTGATGTGGATAAGAGGTTAACCAAGGTAGTTTCTGTCCTCTCCACAAACCCTCCTCATTGTCCACAGCTTTACCTGTACAGTACGGGTGATAAAGTCGTCCCACACCGATCCATTGAATCCTTCATCGCAGAGCAAAGGCAGATAGGTAGACCCGTGAAGTCCTTCAATTTCAGCACATCTCCTCACGTCGACCATTACAGGACTTTTCCCAACATATACTCTCAGGAGCTTAACAATTTCTTGAAAGAATGCTTGGCTACAACAGTTAAGCAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

397

Amino Acids

44.65

Weight (kDa)

9.53

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 118 - 385 1e-64 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18245
fragaria_vesca FvH4_1g13010
malus_domestica MD02G1144600.v1.1 MD15G1258300.v1.1
prunus_persica Prupe.7G157000_v2.0.a1 Prupe.7G157000_v2.0.a1
pyrus_communis pycom02g11450 pycom15g22610
rosa_chinensis RchiOBHm_Chr2g0101161
rosa_laevigata RLG00000017051
rosa_roxburghii Rroxscaffold_2G00141530
rosa_rugosa Rorug02G0100600
rosa_samantha Rh2AG144000 Rh2BG149000 Rh2CG150300 Rh2DG149500
rosa_wichuraiana Rw2G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 526
AccBSI CCGCTC 3 cut(s) 59, 293, 547
AccI GTMKAC 2 cut(s) 1060, 1101
AccII CGCG 1 cut(s) 509
AciI CCGC 8 cut(s) 34, 57, 293, 303, 343, 502, 509, 547
AclWI GGATC 2 cut(s) 701, 1014
AcsI RAATTY 3 cut(s) 77, 197, 630
AcuI CTGAAG 1 cut(s) 416
AcyI GRCGYC 3 cut(s) 50, 132, 270
AdeI CACNNNGTG 1 cut(s) 439
AfaI GTAC 4 cut(s) 404, 416, 989, 994
AfiI CCNNNNNNNGG 4 cut(s) 120, 342, 343, 526
AflIII ACRYGT 1 cut(s) 585
AgsI TTSAA 8 cut(s) 196, 202, 244, 629, 857, 1028, 1078, 1156
AjiI CACGTC 1 cut(s) 1099
AjuI GAANNNNNNNTTGG 2 cut(s) 1148, 1180
AluBI AGCT 9 cut(s) 27, 514, 526, 692, 759, 819, 886, 979, 1141
AluI AGCT 9 cut(s) 27, 514, 526, 692, 759, 819, 886, 979, 1141
Alw21I GWGCWC 1 cut(s) 471
Alw26I GTCTC 2 cut(s) 544, 776
AlwI GGATC 2 cut(s) 701, 1014
AlwNI CAGNNNCTG 1 cut(s) 692
Ama87I CYCGRG 1 cut(s) 423
AoxI GGCC 3 cut(s) 333, 484, 516
ApaI GGGCCC 1 cut(s) 337
ApeKI GCWGC 3 cut(s) 722, 756, 812
ApoI RAATTY 3 cut(s) 77, 197, 630
Asp700I GAANNNNTTC 2 cut(s) 887, 1073
AspS9I GGNCC 6 cut(s) 295, 323, 333, 334, 484, 517
AsuC2I CCSGG 1 cut(s) 491
AsuHPI GGTGA 2 cut(s) 74, 1010
AsuII TTCGAA 1 cut(s) 179
AvaI CYCGRG 1 cut(s) 423
AvaII GGWCC 2 cut(s) 295, 323
AxyI CCTNAGG 1 cut(s) 711
BaeGI GKGCMC 1 cut(s) 337
BanII GRGCYC 2 cut(s) 337, 700
Bbv12I GWGCWC 1 cut(s) 471
BbvCI CCTCAGC 1 cut(s) 23
BbvI GCAGC 3 cut(s) 734, 768, 824
BccI CCATC 1 cut(s) 276
BceAI ACGGC 2 cut(s) 37, 119
BclI TGATCA 1 cut(s) 654
BcnI CCSGG 1 cut(s) 491
BcoDI GTCTC 2 cut(s) 544, 776
BisI GCNGC 4 cut(s) 304, 723, 757, 813
BlsI GCNGC 4 cut(s) 305, 724, 758, 814
Bme1390I CCNGG 1 cut(s) 491
Bme18I GGWCC 2 cut(s) 295, 323
BmeT110I CYCGRG 1 cut(s) 423
BmgBI CACGTC 1 cut(s) 1099
BmgT120I GGNCC 6 cut(s) 295, 323, 333, 334, 484, 517
BmiI GGNNCC 6 cut(s) 287, 324, 334, 335, 697, 843
BmrFI CCNGG 1 cut(s) 491
BmrI ACTGGG 1 cut(s) 374
BmuI ACTGGG 1 cut(s) 374
BpmI CTGGAG 3 cut(s) 714, 825, 831
Bpu10I CCTNAGC 1 cut(s) 23
Bpu14I TTCGAA 1 cut(s) 179
BpuMI CCSGG 1 cut(s) 491
BsaAI YACGTR 1 cut(s) 406
BsaHI GRCGYC 3 cut(s) 50, 132, 270
BsaI GGTCTC 1 cut(s) 544
BsaJI CCNNGG 2 cut(s) 424, 933
BsaXI ACNNNNNCTCC 2 cut(s) 946, 976
Bsc4I CCNNNNNNNGG 4 cut(s) 120, 342, 343, 526
Bse118I RCCGGY 1 cut(s) 272
Bse1I ACTGG 1 cut(s) 369
Bse21I CCTNAGG 1 cut(s) 711
Bse3DI GCAATG 1 cut(s) 224
BseDI CCNNGG 2 cut(s) 424, 933
BseGI GGATG 1 cut(s) 460
BseLI CCNNNNNNNGG 4 cut(s) 120, 342, 343, 526
BseMI GCAATG 1 cut(s) 224
BseMII CTCAG 3 cut(s) 5, 37, 1148
BseNI ACTGG 1 cut(s) 369
BseRI GAGGAG 3 cut(s) 157, 954, 1083
BseSI GKGCMC 1 cut(s) 337
BseXI GCAGC 3 cut(s) 734, 768, 824
BsgI GTGCAG 1 cut(s) 831
Bsh1236I CGCG 1 cut(s) 509
BshFI GGCC 3 cut(s) 335, 486, 518
BsiHKAI GWGCWC 1 cut(s) 471
BsiHKCI CYCGRG 1 cut(s) 423
BsiSI CCGG 3 cut(s) 121, 273, 490
BslFI GGGAC 2 cut(s) 336, 995
BslI CCNNNNNNNGG 4 cut(s) 120, 342, 343, 526
BsmAI GTCTC 2 cut(s) 544, 776
BsmFI GGGAC 2 cut(s) 336, 995
BsmI GAATGC 1 cut(s) 1166
BsnI GGCC 3 cut(s) 335, 486, 518
Bso31I GGTCTC 1 cut(s) 544
BsoBI CYCGRG 1 cut(s) 423
Bsp119I TTCGAA 1 cut(s) 179
Bsp120I GGGCCC 1 cut(s) 333
Bsp1286I GDGCHC 3 cut(s) 337, 471, 700
Bsp1407I TGTACA 1 cut(s) 987
Bsp143I GATC 4 cut(s) 231, 654, 706, 1019
BspACI CCGC 8 cut(s) 34, 57, 293, 303, 343, 502, 509, 547
BspANI GGCC 3 cut(s) 335, 486, 518
BspCNI CTCAG 3 cut(s) 6, 36, 1147
BspFNI CGCG 1 cut(s) 509
BspLI GGNNCC 6 cut(s) 287, 324, 334, 335, 697, 843
BspPI GGATC 2 cut(s) 701, 1014
BspT104I TTCGAA 1 cut(s) 179
BspTNI GGTCTC 1 cut(s) 544
BsrBI CCGCTC 3 cut(s) 59, 293, 547
BsrDI GCAATG 1 cut(s) 224
BsrFI RCCGGY 1 cut(s) 272
BsrGI TGTACA 1 cut(s) 987
BsrI ACTGG 1 cut(s) 369
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 2 cut(s) 424, 933
BssMI GATC 4 cut(s) 231, 654, 706, 1019
BssNI GRCGYC 3 cut(s) 50, 132, 270
BssT1I CCWWGG 1 cut(s) 933
Bst4CI ACNGT 4 cut(s) 355, 570, 992, 1177
Bst6I CTCTTC 2 cut(s) 392, 831
BstACI GRCGYC 3 cut(s) 50, 132, 270
BstAUI TGTACA 1 cut(s) 987
BstBAI YACGTR 1 cut(s) 406
BstBI TTCGAA 1 cut(s) 179
BstC8I GCNNGC 4 cut(s) 371, 516, 528, 817
BstDEI CTNAG 4 cut(s) 14, 23, 711, 1134
BstF5I GGATG 1 cut(s) 460
BstFNI CGCG 1 cut(s) 509
BstKTI GATC 4 cut(s) 234, 657, 709, 1022
BstMAI GTCTC 2 cut(s) 544, 776
BstMBI GATC 4 cut(s) 231, 654, 706, 1019
BstMWI GCNNNNNNNGC 3 cut(s) 33, 515, 553
BstSCI CCNGG 1 cut(s) 489
BstSLI GKGCMC 1 cut(s) 337
BstUI CGCG 1 cut(s) 509
BstV1I GCAGC 3 cut(s) 734, 768, 824
Bsu36I CCTNAGG 1 cut(s) 711
BsuRI GGCC 3 cut(s) 335, 486, 518
BtgZI GCGATG 1 cut(s) 1021
BtrI CACGTC 1 cut(s) 1099
BtsCI GGATG 1 cut(s) 460
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 2 cut(s) 11, 735
Cac8I GCNNGC 4 cut(s) 371, 516, 528, 817
CaiI CAGNNNCTG 1 cut(s) 692
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 6 cut(s) 295, 323, 333, 334, 484, 517
CseI GACGC 3 cut(s) 58, 140, 278
Csp6I GTAC 4 cut(s) 403, 415, 988, 993
CspCI CAANNNNNGTGG 2 cut(s) 756, 791
CviAII CATG 2 cut(s) 521, 862
CviQI GTAC 4 cut(s) 403, 415, 988, 993
DdeI CTNAG 4 cut(s) 14, 23, 711, 1134
DpnI GATC 4 cut(s) 233, 656, 708, 1021
DpnII GATC 4 cut(s) 231, 654, 706, 1019
DraIII CACNNNGTG 1 cut(s) 439
Eam1104I CTCTTC 2 cut(s) 392, 831
EarI CTCTTC 2 cut(s) 392, 831
Eco130I CCWWGG 1 cut(s) 933
Eco24I GRGCYC 2 cut(s) 337, 700
Eco31I GGTCTC 1 cut(s) 544
Eco47I GGWCC 2 cut(s) 295, 323
Eco57I CTGAAG 1 cut(s) 416
Eco81I CCTNAGG 1 cut(s) 711
Eco88I CYCGRG 1 cut(s) 423
EcoO109I RGGNCCY 2 cut(s) 323, 333
EcoT14I CCWWGG 1 cut(s) 933
EcoT38I GRGCYC 2 cut(s) 337, 700
ErhI CCWWGG 1 cut(s) 933
FaeI CATG 2 cut(s) 524, 865
FaiI YATR 7 cut(s) 522, 613, 615, 746, 863, 1127, 1129
FaqI GGGAC 2 cut(s) 336, 995
FatI CATG 2 cut(s) 520, 861
FauI CCCGC 3 cut(s) 336, 495, 540
FbaI TGATCA 1 cut(s) 654
FblI GTMKAC 2 cut(s) 1060, 1101
Fnu4HI GCNGC 4 cut(s) 304, 723, 757, 813
FokI GGATG 1 cut(s) 467
FriOI GRGCYC 2 cut(s) 337, 700
Fsp4HI GCNGC 4 cut(s) 304, 723, 757, 813
GluI GCNGC 4 cut(s) 304, 723, 757, 813
GsuI CTGGAG 3 cut(s) 714, 825, 831
HaeIII GGCC 3 cut(s) 335, 486, 518
HapII CCGG 3 cut(s) 121, 273, 490
HgaI GACGC 3 cut(s) 58, 140, 278
Hin1I GRCGYC 3 cut(s) 50, 132, 270
Hin1II CATG 2 cut(s) 524, 865
HincII GTYRAC 2 cut(s) 930, 1102
HindII GTYRAC 2 cut(s) 930, 1102
HinfI GANTC 6 cut(s) 7, 18, 181, 683, 729, 1028
HpaI GTTAAC 1 cut(s) 930
HpaII CCGG 3 cut(s) 121, 273, 490
HphI GGTGA 2 cut(s) 74, 1010
Hpy166II GTNNAC 5 cut(s) 298, 930, 974, 1061, 1102
Hpy188I TCNGA 3 cut(s) 281, 384, 544
Hpy188III TCNNGA 6 cut(s) 423, 658, 687, 804, 1136, 1153
Hpy8I GTNNAC 5 cut(s) 298, 930, 974, 1061, 1102
Hpy99I CGWCG 4 cut(s) 52, 134, 272, 1103
HpyAV CCTTC 4 cut(s) 181, 454, 1042, 1084
HpyCH4III ACNGT 4 cut(s) 355, 570, 992, 1177
HpyCH4IV ACGT 4 cut(s) 405, 443, 587, 1098
HpyCH4V TGCA 5 cut(s) 107, 224, 620, 812, 832
HpyF10VI GCNNNNNNNGC 3 cut(s) 33, 515, 553
HpyF3I CTNAG 4 cut(s) 14, 23, 711, 1134
HpySE526I ACGT 4 cut(s) 405, 443, 587, 1098
Hsp92I GRCGYC 3 cut(s) 50, 132, 270
Hsp92II CATG 2 cut(s) 524, 865
Ksp22I TGATCA 1 cut(s) 654
KspAI GTTAAC 1 cut(s) 930
Kzo9I GATC 4 cut(s) 231, 654, 706, 1019
LmnI GCTCC 5 cut(s) 511, 544, 689, 695, 1138
Lsp1109I GCAGC 3 cut(s) 734, 768, 824
MaeII ACGT 4 cut(s) 405, 443, 587, 1098
MaeIII GTNAC 3 cut(s) 349, 360, 439
MalI GATC 4 cut(s) 233, 656, 708, 1021
MbiI CCGCTC 3 cut(s) 59, 293, 547
MboI GATC 4 cut(s) 231, 654, 706, 1019
MboII GAAGA 4 cut(s) 58, 409, 760, 848
MfeI CAATTG 1 cut(s) 852
MhlI GDGCHC 3 cut(s) 337, 471, 700
MroXI GAANNNNTTC 2 cut(s) 887, 1073
MseI TTAA 6 cut(s) 30, 666, 794, 929, 1143, 1179
MspA1I CMGCKG 1 cut(s) 303
MspI CCGG 3 cut(s) 121, 273, 490
MspR9I CCNGG 1 cut(s) 491
MunI CAATTG 1 cut(s) 852
Mva1269I GAATGC 1 cut(s) 1166
MvnI CGCG 1 cut(s) 509
MwoI GCNNNNNNNGC 3 cut(s) 33, 515, 553
NciI CCSGG 1 cut(s) 491
NdeII GATC 4 cut(s) 231, 654, 706, 1019
NlaIII CATG 2 cut(s) 524, 865
NlaIV GGNNCC 6 cut(s) 287, 324, 334, 335, 697, 843
NmuCI GTSAC 2 cut(s) 349, 439
NspV TTCGAA 1 cut(s) 179
PctI GAATGC 1 cut(s) 1166
PdmI GAANNNNTTC 2 cut(s) 887, 1073
PfeI GAWTC 5 cut(s) 18, 181, 683, 729, 1028
PflFI GACNNNGTC 1 cut(s) 52
PflMI CCANNNNNTGG 1 cut(s) 526
PkrI GCNGC 4 cut(s) 305, 724, 758, 814
Ppu21I YACGTR 1 cut(s) 406
PpuMI RGGWCCY 1 cut(s) 323
Psp5II RGGWCCY 1 cut(s) 323
PspN4I GGNNCC 6 cut(s) 287, 324, 334, 335, 697, 843
PspOMI GGGCCC 1 cut(s) 333
PspPI GGNCC 6 cut(s) 295, 323, 333, 334, 484, 517
PspPPI RGGWCCY 1 cut(s) 323
PstNI CAGNNNCTG 1 cut(s) 692
PsyI GACNNNGTC 1 cut(s) 52
RsaI GTAC 4 cut(s) 404, 416, 989, 994
RsaNI GTAC 4 cut(s) 403, 415, 988, 993
SalI GTCGAC 1 cut(s) 1100
SaqAI TTAA 6 cut(s) 30, 666, 794, 929, 1143, 1179
SatI GCNGC 4 cut(s) 304, 723, 757, 813
Sau3AI GATC 4 cut(s) 231, 654, 706, 1019
Sau96I GGNCC 6 cut(s) 295, 323, 333, 334, 484, 517
ScrFI CCNGG 1 cut(s) 491
SduI GDGCHC 3 cut(s) 337, 471, 700
SfuI TTCGAA 1 cut(s) 179
SinI GGWCC 2 cut(s) 295, 323
SsiI CCGC 8 cut(s) 34, 57, 293, 303, 343, 502, 509, 547
StyD4I CCNGG 1 cut(s) 489
StyI CCWWGG 1 cut(s) 933
TaaI ACNGT 4 cut(s) 355, 570, 992, 1177
TaiI ACGT 4 cut(s) 408, 446, 590, 1101
TaqI TCGA 4 cut(s) 129, 179, 257, 1101
TatI WGTACW 1 cut(s) 987
TauI GCSGC 1 cut(s) 306
TfiI GAWTC 5 cut(s) 18, 181, 683, 729, 1028
Tru1I TTAA 6 cut(s) 30, 666, 794, 929, 1143, 1179
Tru9I TTAA 6 cut(s) 30, 666, 794, 929, 1143, 1179
TscAI CASTG 2 cut(s) 18, 742
TseFI GTSAC 2 cut(s) 349, 439
TseI GCWGC 3 cut(s) 722, 756, 812
Tsp45I GTSAC 2 cut(s) 349, 439
TspDTI ATGAA 2 cut(s) 761, 1024
TspGWI ACGGA 1 cut(s) 327
TspRI CASTG 2 cut(s) 18, 742
Tth111I GACNNNGTC 1 cut(s) 52
Van91I CCANNNNNTGG 1 cut(s) 526
VpaK11BI GGWCC 2 cut(s) 295, 323
XapI RAATTY 3 cut(s) 77, 197, 630
XcmI CCANNNNNNNNNTGG 1 cut(s) 868
XmiI GTMKAC 2 cut(s) 1060, 1101
XmnI GAANNNNTTC 2 cut(s) 887, 1073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.