Prupe.7G157000_v2.0.a1

Transmembrane protein 53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0014260
Physical Location & Seq
Reverse (-)
16539399 .. 16541939
2541 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G157000.1

Sequence Viewer

Length: 1215 bp
ATGGAAACTCCAGTGAGAATTCTCAGTCTAAATCGCCAGCTTTTCGCCAAAATGACGCCGTCCGCTCTTACGTTCTCTCCCAAACTTCTCCGACCCCCAAATCCCTTACTTCCATTTCACACTGCCGCTCGAAACGGCGTCGTCAAAAGAGCCCCCTTTTCTCTTTCTCTCGCACAGCCACAGTCCTCGTTCAACTTCTCCACCCATACAAACCCCTTTCAATCCCTCCTCTTCTCTCTCTCTTCCCAATTCCCGATCAACAACTTCAACCACTCGCTCTCGCACTCACACGACGGCGTTTTCACATGGAACCGAGCTTTCCAGAGTGGCATTGATGGAAATGTGGGTTCTTTCGGAACCCAAAAGCGTGAAGTAACCGTCGTCTTGCTGGGTTGGCTTGGGGCCAAGACCAAGCACCTGAAGAAGTACGTGGAGTGGTATAACTCCAGAGGCATTCACGCCGTGACCTTCGTTGTTGATGCGAGGGAGGTGCTCTGGTTTGACTTGGGCCACCGGGTCGAAAAACGGGTTTCGGATTTGGCCCACGAGCTCGTTTCATGGGTGGAGGAGAGGGTAGAGGATGGGAGAGAAAGGTGTTTGCTTTTTCACACTTTCAGCAATACGGGTTGGTTTGTATATGGCGCCATTCTTGAAATTCTTCAGGGTAGACAAGACCTGATGGAGAAGATTAAAGGATGTATTGTTGATTCAGGAGCAGCGGAGCCTTTTAATCCTAAGGTGTGGGCAGCGGGCTTTTCTGTCGCTATCTTGAAGAAACGCAGCTCTTTAACAAGCCCCGCGGTTGAGGCTAGAGAACTGAATAAGTCTGAAGATGCAGTGAGCTTGTCAAAGATGCAAGAAGAGAAACCTCTGATAGTTGAAACCATGGTTCTGTTGGTATTAGAGAAGCTGTTCTCAGTTCTTCTAAAGTTGCCTGACGTGGACAAGAGGTTGTCGAAGGTAGTTTCTATCCTCTCAGAAAACACTCCTTATTGCCCTCAACTTTACCTGTATAGTACCGCTGATGGAGTTGTTCCGCATCAGTCGATCGAGTTGTTCATTGCAGAGCAGAGGAGGAAGGGTAGAATGGTTAGGTCCTTCAATTTCGGCTCATCACCCCATGTAGACCACTTCAGGACTTTCCCCAACATCTACTCTTCAAAGCTCGACTGTTTCTTGAAGGATTGTTTAGCTACCTCTAAGACGACAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

405

Amino Acids

45.57

Weight (kDa)

9.32

Isoelectric Point (pI)

43.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18245
fragaria_vesca FvH4_1g13010
malus_domestica MD02G1144600.v1.1 MD15G1258300.v1.1
prunus_persica Prupe.7G157000_v2.0.a1 Prupe.7G157000_v2.0.a1
pyrus_communis pycom02g11450 pycom15g22610
rosa_chinensis RchiOBHm_Chr2g0101161
rosa_laevigata RLG00000017051
rosa_roxburghii Rroxscaffold_2G00141530
rosa_rugosa Rorug02G0100600
rosa_samantha Rh2AG144000 Rh2BG149000 Rh2CG150300 Rh2DG149500
rosa_wichuraiana Rw2G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 641
AccBSI CCGCTC 2 cut(s) 65, 128
AccI GTMKAC 2 cut(s) 667, 1125
AccII CGCG 1 cut(s) 800
AciI CCGC 8 cut(s) 63, 126, 719, 749, 798, 800, 1020, 1037
AcsI RAATTY 2 cut(s) 18, 654
AcuI CTGAAG 4 cut(s) 440, 644, 849, 1117
AcyI GRCGYC 3 cut(s) 56, 138, 642
AdeI CACNNNGTG 1 cut(s) 463
AfaI GTAC 2 cut(s) 428, 1018
AgsI TTSAA 9 cut(s) 193, 221, 268, 653, 772, 881, 1102, 1161, 1180
AjiI CACGTC 1 cut(s) 940
AluBI AGCT 8 cut(s) 40, 317, 550, 783, 843, 910, 1165, 1193
AluI AGCT 8 cut(s) 40, 317, 550, 783, 843, 910, 1165, 1193
Alw21I GWGCWC 2 cut(s) 495, 552
AoxI GGCC 3 cut(s) 402, 508, 540
ApeKI GCWGC 3 cut(s) 716, 746, 780
ApoI RAATTY 2 cut(s) 18, 654
Asp700I GAANNNNTTC 2 cut(s) 657, 911
AspLEI GCGC 1 cut(s) 644
AspS9I GGNCC 4 cut(s) 402, 508, 541, 1095
AsuC2I CCSGG 1 cut(s) 515
AsuHPI GGTGA 1 cut(s) 1107
AvaII GGWCC 1 cut(s) 1095
AxyI CCTNAGG 1 cut(s) 735
BanI GGYRCC 1 cut(s) 641
BanII GRGCYC 2 cut(s) 154, 552
BauI CACGAG 1 cut(s) 545
Bbv12I GWGCWC 2 cut(s) 495, 552
BbvI GCAGC 3 cut(s) 728, 758, 792
BccI CCATC 4 cut(s) 329, 575, 673, 1019
BceAI ACGGC 4 cut(s) 43, 151, 310, 446
BcgI CGANNNNNNTGC 2 cut(s) 472, 506
BcnI CCSGG 1 cut(s) 515
BfaI CTAG 1 cut(s) 810
BfoI RGCGCY 1 cut(s) 645
BisI GCNGC 4 cut(s) 126, 717, 747, 781
BlsI GCNGC 4 cut(s) 127, 718, 748, 782
Bme1390I CCNGG 1 cut(s) 515
Bme18I GGWCC 1 cut(s) 1095
BmgBI CACGTC 1 cut(s) 940
BmgT120I GGNCC 4 cut(s) 402, 508, 541, 1095
BmiI GGNNCC 5 cut(s) 311, 358, 403, 643, 723
BmrFI CCNGG 1 cut(s) 515
BmsI GCATC 4 cut(s) 469, 823, 843, 1048
BpmI CTGGAG 1 cut(s) 430
BpuMI CCSGG 1 cut(s) 515
BsaAI YACGTR 1 cut(s) 430
BsaHI GRCGYC 3 cut(s) 56, 138, 642
BsaJI CCNNGG 2 cut(s) 798, 885
BsaXI ACNNNNNCTCC 4 cut(s) 61, 91, 1066, 1096
Bse1I ACTGG 1 cut(s) 11
Bse21I CCTNAGG 1 cut(s) 735
Bse3DI GCAATG 1 cut(s) 1059
BseDI CCNNGG 2 cut(s) 798, 885
BseGI GGATG 2 cut(s) 586, 701
BseMI GCAATG 1 cut(s) 1059
BseMII CTCAG 3 cut(s) 37, 930, 990
BseNI ACTGG 1 cut(s) 11
BseRI GAGGAG 3 cut(s) 218, 581, 1087
BseXI GCAGC 3 cut(s) 728, 758, 792
BseYI CCCAGC 1 cut(s) 388
Bsh1236I CGCG 1 cut(s) 800
Bsh1285I CGRYCG 1 cut(s) 1050
BshFI GGCC 3 cut(s) 404, 510, 542
BshNI GGYRCC 1 cut(s) 641
BsiEI CGRYCG 1 cut(s) 1050
BsiHKAI GWGCWC 2 cut(s) 495, 552
BsiSI CCGG 1 cut(s) 514
BsmI GAATGC 1 cut(s) 453
BsnI GGCC 3 cut(s) 404, 510, 542
Bsp1286I GDGCHC 3 cut(s) 154, 495, 552
Bsp143I GATC 2 cut(s) 255, 1047
Bsp19I CCATGG 1 cut(s) 885
BspACI CCGC 8 cut(s) 63, 126, 719, 749, 798, 800, 1020, 1037
BspANI GGCC 3 cut(s) 404, 510, 542
BspCNI CTCAG 3 cut(s) 36, 929, 989
BspFNI CGCG 1 cut(s) 800
BspLI GGNNCC 5 cut(s) 311, 358, 403, 643, 723
BspT107I GGYRCC 1 cut(s) 641
BsrBI CCGCTC 2 cut(s) 65, 128
BsrDI GCAATG 1 cut(s) 1059
BsrI ACTGG 1 cut(s) 11
BssECI CCNNGG 2 cut(s) 798, 885
BssMI GATC 2 cut(s) 255, 1047
BssNI GRCGYC 3 cut(s) 56, 138, 642
BssSI CACGAG 1 cut(s) 545
BssT1I CCWWGG 1 cut(s) 885
Bst2BI CACGAG 1 cut(s) 545
Bst4CI ACNGT 3 cut(s) 183, 379, 1172
Bst6I CTCTTC 4 cut(s) 236, 247, 855, 1162
BstACI GRCGYC 3 cut(s) 56, 138, 642
BstBAI YACGTR 1 cut(s) 430
BstC8I GCNNGC 2 cut(s) 38, 751
BstDEI CTNAG 5 cut(s) 23, 735, 916, 976, 1200
BstDSI CCRYGG 2 cut(s) 798, 885
BstF5I GGATG 2 cut(s) 586, 701
BstFNI CGCG 1 cut(s) 800
BstH2I RGCGCY 1 cut(s) 645
BstHHI GCGC 1 cut(s) 644
BstKTI GATC 2 cut(s) 258, 1050
BstMBI GATC 2 cut(s) 255, 1047
BstMCI CGRYCG 1 cut(s) 1050
BstMWI GCNNNNNNNGC 2 cut(s) 394, 806
BstSCI CCNGG 1 cut(s) 513
BstUI CGCG 1 cut(s) 800
BstV1I GCAGC 3 cut(s) 728, 758, 792
Bsu36I CCTNAGG 1 cut(s) 735
BsuRI GGCC 3 cut(s) 404, 510, 542
BtgI CCRYGG 2 cut(s) 798, 885
BtrI CACGTC 1 cut(s) 940
BtsCI GGATG 2 cut(s) 586, 701
BtsI GCAGTG 2 cut(s) 120, 843
BtsIMutI CAGTG 3 cut(s) 18, 120, 843
Cac8I GCNNGC 2 cut(s) 38, 751
CfoI GCGC 1 cut(s) 644
Cfr13I GGNCC 4 cut(s) 402, 508, 541, 1095
Cfr42I CCGCGG 1 cut(s) 801
CseI GACGC 2 cut(s) 64, 127
Csp6I GTAC 2 cut(s) 427, 1017
CviAII CATG 4 cut(s) 306, 558, 886, 1121
CviQI GTAC 2 cut(s) 427, 1017
DdeI CTNAG 5 cut(s) 23, 735, 916, 976, 1200
DinI GGCGCC 1 cut(s) 643
DpnI GATC 2 cut(s) 257, 1049
DpnII GATC 2 cut(s) 255, 1047
DraIII CACNNNGTG 1 cut(s) 463
Eam1104I CTCTTC 4 cut(s) 236, 247, 855, 1162
EarI CTCTTC 4 cut(s) 236, 247, 855, 1162
Ecl136II GAGCTC 1 cut(s) 550
Eco130I CCWWGG 1 cut(s) 885
Eco24I GRGCYC 2 cut(s) 154, 552
Eco47I GGWCC 1 cut(s) 1095
Eco53kI GAGCTC 1 cut(s) 550
Eco57I CTGAAG 4 cut(s) 440, 644, 849, 1117
Eco81I CCTNAGG 1 cut(s) 735
EcoICRI GAGCTC 1 cut(s) 550
EcoO109I RGGNCCY 1 cut(s) 1095
EcoRI GAATTC 1 cut(s) 18
EcoT14I CCWWGG 1 cut(s) 885
EcoT38I GRGCYC 2 cut(s) 154, 552
EgeI GGCGCC 1 cut(s) 643
EheI GGCGCC 1 cut(s) 643
ErhI CCWWGG 1 cut(s) 885
FaeI CATG 4 cut(s) 309, 561, 889, 1124
FatI CATG 4 cut(s) 305, 557, 885, 1120
FauI CCCGC 2 cut(s) 742, 805
FblI GTMKAC 2 cut(s) 667, 1125
Fnu4HI GCNGC 4 cut(s) 126, 717, 747, 781
FokI GGATG 2 cut(s) 593, 708
FriOI GRGCYC 2 cut(s) 154, 552
Fsp4HI GCNGC 4 cut(s) 126, 717, 747, 781
FspBI CTAG 1 cut(s) 810
GlaI GCGC 1 cut(s) 643
GluI GCNGC 4 cut(s) 126, 717, 747, 781
GsaI CCCAGC 1 cut(s) 392
GsuI CTGGAG 1 cut(s) 430
HaeII RGCGCY 1 cut(s) 645
HaeIII GGCC 3 cut(s) 404, 510, 542
HapII CCGG 1 cut(s) 514
HgaI GACGC 2 cut(s) 64, 127
HhaI GCGC 1 cut(s) 644
Hin1I GRCGYC 3 cut(s) 56, 138, 642
Hin1II CATG 4 cut(s) 309, 561, 889, 1124
Hin6I GCGC 1 cut(s) 642
HinP1I GCGC 1 cut(s) 642
HinfI GANTC 1 cut(s) 707
HpaII CCGG 1 cut(s) 514
HphI GGTGA 1 cut(s) 1107
Hpy166II GTNNAC 3 cut(s) 668, 943, 1126
Hpy188I TCNGA 6 cut(s) 92, 356, 535, 829, 873, 979
Hpy188III TCNNGA 8 cut(s) 253, 322, 447, 650, 711, 769, 1135, 1177
Hpy8I GTNNAC 3 cut(s) 668, 943, 1126
Hpy99I CGWCG 3 cut(s) 143, 296, 383
HpyAV CCTTC 5 cut(s) 478, 952, 1072, 1108, 1174
HpyCH4III ACNGT 3 cut(s) 183, 379, 1172
HpyCH4IV ACGT 3 cut(s) 71, 429, 939
HpyCH4V TGCA 3 cut(s) 836, 856, 1064
HpyF10VI GCNNNNNNNGC 2 cut(s) 394, 806
HpyF3I CTNAG 5 cut(s) 23, 735, 916, 976, 1200
HpySE526I ACGT 3 cut(s) 71, 429, 939
Hsp92I GRCGYC 3 cut(s) 56, 138, 642
Hsp92II CATG 4 cut(s) 309, 561, 889, 1124
HspAI GCGC 1 cut(s) 642
KasI GGCGCC 1 cut(s) 641
KspI CCGCGG 1 cut(s) 801
Kzo9I GATC 2 cut(s) 255, 1047
LmnI GCTCC 2 cut(s) 713, 721
Lsp1109I GCAGC 3 cut(s) 728, 758, 792
LweI GCATC 4 cut(s) 469, 823, 843, 1048
MaeI CTAG 1 cut(s) 810
MaeII ACGT 3 cut(s) 71, 429, 939
MaeIII GTNAC 2 cut(s) 373, 463
MalI GATC 2 cut(s) 257, 1049
MbiI CCGCTC 2 cut(s) 65, 128
MboI GATC 2 cut(s) 255, 1047
MhlI GDGCHC 3 cut(s) 154, 495, 552
MluCI AATT 4 cut(s) 18, 248, 654, 1102
Mly113I GGCGCC 1 cut(s) 642
MmeI TCCRAC 1 cut(s) 115
MroXI GAANNNNTTC 2 cut(s) 657, 911
MseI TTAA 3 cut(s) 690, 729, 788
MspA1I CMGCKG 4 cut(s) 719, 749, 800, 1022
MspI CCGG 1 cut(s) 514
MspR9I CCNGG 1 cut(s) 515
Mva1269I GAATGC 1 cut(s) 453
MvnI CGCG 1 cut(s) 800
MwoI GCNNNNNNNGC 2 cut(s) 394, 806
NarI GGCGCC 1 cut(s) 642
NciI CCSGG 1 cut(s) 515
NcoI CCATGG 1 cut(s) 885
NdeII GATC 2 cut(s) 255, 1047
NlaIII CATG 4 cut(s) 309, 561, 889, 1124
NlaIV GGNNCC 5 cut(s) 311, 358, 403, 643, 723
NmuCI GTSAC 1 cut(s) 463
PctI GAATGC 1 cut(s) 453
PdmI GAANNNNTTC 2 cut(s) 657, 911
PfeI GAWTC 1 cut(s) 707
PflFI GACNNNGTC 1 cut(s) 58
PkrI GCNGC 4 cut(s) 127, 718, 748, 782
Ple19I CGATCG 1 cut(s) 1050
PluTI GGCGCC 1 cut(s) 645
Ppu21I YACGTR 1 cut(s) 430
PpuMI RGGWCCY 1 cut(s) 1095
Psp124BI GAGCTC 1 cut(s) 552
Psp5II RGGWCCY 1 cut(s) 1095
PspFI CCCAGC 1 cut(s) 388
PspN4I GGNNCC 5 cut(s) 311, 358, 403, 643, 723
PspPI GGNCC 4 cut(s) 402, 508, 541, 1095
PspPPI RGGWCCY 1 cut(s) 1095
PsyI GACNNNGTC 1 cut(s) 58
PvuI CGATCG 1 cut(s) 1050
RsaI GTAC 2 cut(s) 428, 1018
RsaNI GTAC 2 cut(s) 427, 1017
SacI GAGCTC 1 cut(s) 552
SacII CCGCGG 1 cut(s) 801
SaqAI TTAA 3 cut(s) 690, 729, 788
SatI GCNGC 4 cut(s) 126, 717, 747, 781
Sau3AI GATC 2 cut(s) 255, 1047
Sau96I GGNCC 4 cut(s) 402, 508, 541, 1095
ScrFI CCNGG 1 cut(s) 515
SduI GDGCHC 3 cut(s) 154, 495, 552
SfaNI GCATC 4 cut(s) 469, 823, 843, 1048
SfoI GGCGCC 1 cut(s) 643
Sfr303I CCGCGG 1 cut(s) 801
SgrBI CCGCGG 1 cut(s) 801
SinI GGWCC 1 cut(s) 1095
Sse9I AATT 4 cut(s) 18, 248, 654, 1102
SsiI CCGC 8 cut(s) 63, 126, 719, 749, 798, 800, 1020, 1037
SspDI GGCGCC 1 cut(s) 641
SspMI CTAG 1 cut(s) 810
SstI GAGCTC 1 cut(s) 552
StyD4I CCNGG 1 cut(s) 513
StyI CCWWGG 1 cut(s) 885
TaaI ACNGT 3 cut(s) 183, 379, 1172
TaiI ACGT 3 cut(s) 74, 432, 942
TaqI TCGA 6 cut(s) 130, 519, 956, 1046, 1050, 1167
TasI AATT 4 cut(s) 18, 248, 654, 1102
TauI GCSGC 1 cut(s) 128
TfiI GAWTC 1 cut(s) 707
Tru1I TTAA 3 cut(s) 690, 729, 788
Tru9I TTAA 3 cut(s) 690, 729, 788
TscAI CASTG 3 cut(s) 18, 127, 843
TseFI GTSAC 1 cut(s) 463
TseI GCWGC 3 cut(s) 716, 746, 780
Tsp45I GTSAC 1 cut(s) 463
TspDTI ATGAA 2 cut(s) 546, 1048
TspRI CASTG 3 cut(s) 18, 127, 843
Tth111I GACNNNGTC 1 cut(s) 58
VpaK11BI GGWCC 1 cut(s) 1095
XapI RAATTY 2 cut(s) 18, 654
XcmI CCANNNNNNNNNTGG 1 cut(s) 892
XmiI GTMKAC 2 cut(s) 667, 1125
XmnI GAANNNNTTC 2 cut(s) 657, 911
XspI CTAG 1 cut(s) 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.