pycom02g11450

Transmembrane protein 53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0014260
Physical Location & Seq
Forward (+)
8085104 .. 8087135
2032 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g11450.2

Sequence Viewer

Length: 1176 bp
ATGGAAACTCCGGCGAGAATTCTCAGCCTCAACCTCCAGCTCTTCACCAGAACGACACCGTCCGCCCTTGCTTTCTCTCCCAAACTCCACAGACCCGCGAAGCCCTCGCCTACATTTCACACTCTCGCTCGAAACGGGGTCGTCAAAAGAACCCCCTTTTCTCTTTCGCTCGCGCAGCCGCACTCCTCGTTCTCCTTCAATCCGAACCCCTTCAAATCCCTCCCCCTTTCTCTCTCCTCCCCGTCGCCGTTCAGCAACTTCAACCACTCTCTCTCCCCCTCAAACGACGGTGTTCCCACCTGGAACTGGGCGTTCCACGGCGGCATTAACGGAAATGTGGGTTCTTTCGGGACCCACAACCGTGAAGTGACGGTGGTGTTGCTAGGCTGGCTCGGGGCCAAGACGAAGCACCTAAAGAGGTACGTGGAGTGGTACAACTCCAGAGGCATTCACGCCGTGACCTTCGTCGTTGATCCGAGGGAGGTTCTCTGGTTTGATTTGAGCCGCCGGGTCGAAAATCGGGTTTCGGACTTGGCCCGGGATCTCGCTTCGTGGGTGGAGGAGGGCGGAGAAAGGTGCTTGCTTTTCCACACCTTCAGCAACACGGGTTGGTTTGTGAATGTTTATGAATCTAGTTGGCCAGATATTTTGCTTTTTATCGCTTTATGTTTAGTTTTATGGTTTTACCTCAATGTGTGGGCAGCAGGATTTTCTGCAGCAATTTTGAAGAAACGCAGCTCTTTAACAAGCTCTGCAGTTGAGGGCCTTGAGGCTAGAGATTTAAATGAGTCAGAAGATGCGGCAAGCTTGTCAAAGCTGCAAGAAGATAAGCCTCCACTAGTTGAATCCGTGGTTCTGCTGGTATTAGAGAAGCTGTTTTCGGTTCTTCTAAAGTTTCCTGATGTGGATAAGAGGTTAATGAAGGTAGTTTCTATTCTTTCGGAAAACACTCCTTATTGCCCGCAACTTTACCTATATAGCACTGCTGATAAAGTTATTCCACACCAGTCAGTCGAGTTGTTCATGGCGGAGCAAAAGCGGAAGGGTAGAATTGTAAGGTCCTTCAATTTCGGCACAACACCTCATGTGGATCACTTCAGGAATTTCCCCGACATATACTGTTCGGAGCTTGATGGTTTCTTGAAAGATTGTTTGACTACGGTTAAGACGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

392

Amino Acids

44.09

Weight (kDa)

8.86

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18245
fragaria_vesca FvH4_1g13010
malus_domestica MD02G1144600.v1.1 MD15G1258300.v1.1
prunus_persica Prupe.7G157000_v2.0.a1 Prupe.7G157000_v2.0.a1
pyrus_communis pycom02g11450 pycom15g22610
rosa_chinensis RchiOBHm_Chr2g0101161
rosa_laevigata RLG00000017051
rosa_roxburghii Rroxscaffold_2G00141530
rosa_rugosa Rorug02G0100600
rosa_samantha Rh2AG144000 Rh2BG149000 Rh2CG150300 Rh2DG149500
rosa_wichuraiana Rw2G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 98, 173
AclWI GGATC 3 cut(s) 467, 549, 1098
AcoI YGGCCR 1 cut(s) 638
AcsI RAATTY 2 cut(s) 18, 1102
AcuI CTGAAG 2 cut(s) 580, 1081
AdeI CACNNNGTG 1 cut(s) 457
AfaI GTAC 2 cut(s) 422, 434
AfiI CCNNNNNNNGG 1 cut(s) 306
AgsI TTSAA 7 cut(s) 199, 214, 262, 727, 845, 1066, 1144
AhlI ACTAGT 1 cut(s) 838
AjiI CACGTC 1 cut(s) 1173
AjnI CCWGG 1 cut(s) 299
AleI CACNNNNGTG 1 cut(s) 360
AluBI AGCT 7 cut(s) 40, 738, 750, 807, 817, 874, 1129
AluI AGCT 7 cut(s) 40, 738, 750, 807, 817, 874, 1129
AlwI GGATC 3 cut(s) 467, 549, 1098
Ama87I CYCGRG 2 cut(s) 392, 537
AoxI GGCC 4 cut(s) 396, 534, 638, 763
ApeKI GCWGC 5 cut(s) 175, 701, 716, 735, 817
ApoI RAATTY 2 cut(s) 18, 1102
Asp700I GAANNNNTTC 1 cut(s) 209
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 5 cut(s) 351, 396, 535, 763, 1059
AsuC2I CCSGG 3 cut(s) 509, 538, 539
AsuHPI GGTGA 1 cut(s) 37
AvaI CYCGRG 2 cut(s) 392, 537
AvaII GGWCC 2 cut(s) 351, 1059
BalI TGGCCA 1 cut(s) 640
BbvI GCAGC 5 cut(s) 187, 713, 728, 747, 804
BccI CCATC 1 cut(s) 1127
BceAI ACGGC 3 cut(s) 232, 334, 440
BciT130I CCWGG 1 cut(s) 301
BcnI CCSGG 3 cut(s) 509, 538, 539
BcuI ACTAGT 1 cut(s) 838
BfaI CTAG 4 cut(s) 383, 633, 774, 839
BfmI CTRYAG 2 cut(s) 714, 753
BisI GCNGC 9 cut(s) 176, 179, 322, 505, 702, 717, 736, 801, 818
BlsI GCNGC 9 cut(s) 177, 180, 323, 506, 703, 718, 737, 802, 819
Bme1390I CCNGG 4 cut(s) 301, 509, 538, 539
Bme18I GGWCC 2 cut(s) 351, 1059
BmeT110I CYCGRG 2 cut(s) 392, 537
BmgBI CACGTC 1 cut(s) 1173
BmgT120I GGNCC 5 cut(s) 351, 396, 535, 763, 1059
BmiI GGNNCC 3 cut(s) 352, 353, 397
BmrFI CCNGG 4 cut(s) 301, 509, 538, 539
BmrI ACTGGG 1 cut(s) 316
BmsI GCATC 1 cut(s) 787
BmuI ACTGGG 1 cut(s) 316
BoxI GACNNNNGTC 1 cut(s) 464
BpmI CTGGAG 2 cut(s) 20, 424
BpuEI CTTGAG 1 cut(s) 788
BpuMI CCSGG 3 cut(s) 509, 538, 539
BsaAI YACGTR 1 cut(s) 424
BsaJI CCNNGG 4 cut(s) 316, 476, 537, 849
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 1 cut(s) 306
Bse1I ACTGG 2 cut(s) 311, 1006
BseBI CCWGG 1 cut(s) 301
BseDI CCNNGG 4 cut(s) 316, 476, 537, 849
BseLI CCNNNNNNNGG 1 cut(s) 306
BseMII CTCAG 1 cut(s) 37
BseNI ACTGG 2 cut(s) 311, 1006
BseRI GAGGAG 3 cut(s) 175, 226, 575
BseXI GCAGC 5 cut(s) 187, 713, 728, 747, 804
Bsh1236I CGCG 2 cut(s) 98, 173
BshFI GGCC 4 cut(s) 398, 536, 640, 765
BsiHKCI CYCGRG 2 cut(s) 392, 537
BsiSI CCGG 3 cut(s) 11, 508, 538
BslFI GGGAC 1 cut(s) 364
BslI CCNNNNNNNGG 1 cut(s) 306
BsmFI GGGAC 1 cut(s) 364
BsmI GAATGC 1 cut(s) 447
BsnI GGCC 4 cut(s) 398, 536, 640, 765
BsoBI CYCGRG 2 cut(s) 392, 537
Bsp143I GATC 3 cut(s) 472, 541, 1090
BspANI GGCC 4 cut(s) 398, 536, 640, 765
BspCNI CTCAG 1 cut(s) 36
BspFNI CGCG 2 cut(s) 98, 173
BspLI GGNNCC 3 cut(s) 352, 353, 397
BspMAI CTGCAG 2 cut(s) 718, 757
BspPI GGATC 3 cut(s) 467, 549, 1098
BspQI GCTCTTC 1 cut(s) 47
BsrI ACTGG 2 cut(s) 311, 1006
BssECI CCNNGG 4 cut(s) 316, 476, 537, 849
BssMI GATC 3 cut(s) 472, 541, 1090
Bst2UI CCWGG 1 cut(s) 301
Bst4CI ACNGT 6 cut(s) 60, 290, 362, 373, 1121, 1162
Bst6I CTCTTC 1 cut(s) 47
BstBAI YACGTR 1 cut(s) 424
BstC8I GCNNGC 5 cut(s) 171, 389, 581, 805, 962
BstDEI CTNAG 1 cut(s) 23
BstDSI CCRYGG 2 cut(s) 316, 849
BstFNI CGCG 2 cut(s) 98, 173
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 3 cut(s) 475, 544, 1093
BstMBI GATC 3 cut(s) 472, 541, 1090
BstMWI GCNNNNNNNGC 2 cut(s) 175, 388
BstNI CCWGG 1 cut(s) 301
BstPAI GACNNNNGTC 1 cut(s) 464
BstSCI CCNGG 4 cut(s) 299, 507, 536, 537
BstSFI CTRYAG 2 cut(s) 714, 753
BstUI CGCG 2 cut(s) 98, 173
BstV1I GCAGC 5 cut(s) 187, 713, 728, 747, 804
BstX2I RGATCY 1 cut(s) 541
BstYI RGATCY 1 cut(s) 541
BsuRI GGCC 4 cut(s) 398, 536, 640, 765
BtgI CCRYGG 2 cut(s) 316, 849
BtrI CACGTC 1 cut(s) 1173
BtsI GCAGTG 1 cut(s) 981
BtsIMutI CAGTG 1 cut(s) 981
Cac8I GCNNGC 5 cut(s) 171, 389, 581, 805, 962
CfoI GCGC 1 cut(s) 175
Cfr13I GGNCC 5 cut(s) 351, 396, 535, 763, 1059
Cfr9I CCCGGG 1 cut(s) 537
Csp6I GTAC 2 cut(s) 421, 433
CviAII CATG 2 cut(s) 1024, 1085
CviQI GTAC 2 cut(s) 421, 433
DdeI CTNAG 1 cut(s) 23
DpnI GATC 3 cut(s) 474, 543, 1092
DpnII GATC 3 cut(s) 472, 541, 1090
DraI TTTAAA 1 cut(s) 783
DraIII CACNNNGTG 1 cut(s) 457
EaeI YGGCCR 1 cut(s) 638
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
EciI GGCGGA 3 cut(s) 52, 582, 1043
Eco47I GGWCC 2 cut(s) 351, 1059
Eco57I CTGAAG 2 cut(s) 580, 1081
Eco88I CYCGRG 2 cut(s) 392, 537
EcoO109I RGGNCCY 3 cut(s) 351, 763, 1059
EcoRI GAATTC 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 299
FaeI CATG 2 cut(s) 1027, 1088
FaiI YATR 9 cut(s) 627, 667, 679, 976, 978, 1025, 1086, 1115, 1117
FaqI GGGAC 1 cut(s) 364
FatI CATG 2 cut(s) 1023, 1084
FauI CCCGC 2 cut(s) 103, 969
Fnu4HI GCNGC 9 cut(s) 176, 179, 322, 505, 702, 717, 736, 801, 818
Fsp4HI GCNGC 9 cut(s) 176, 179, 322, 505, 702, 717, 736, 801, 818
FspBI CTAG 4 cut(s) 383, 633, 774, 839
GlaI GCGC 1 cut(s) 174
GluI GCNGC 9 cut(s) 176, 179, 322, 505, 702, 717, 736, 801, 818
GsuI CTGGAG 2 cut(s) 20, 424
HaeIII GGCC 4 cut(s) 398, 536, 640, 765
HapII CCGG 3 cut(s) 11, 508, 538
HhaI GCGC 1 cut(s) 175
Hin1II CATG 2 cut(s) 1027, 1088
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HindIII AAGCTT 1 cut(s) 805
HinfI GANTC 3 cut(s) 629, 788, 845
HpaII CCGG 3 cut(s) 11, 508, 538
HphI GGTGA 1 cut(s) 37
Hpy188I TCNGA 6 cut(s) 204, 477, 529, 793, 943, 1126
Hpy188III TCNNGA 5 cut(s) 349, 441, 899, 1099, 1141
Hpy99I CGWCG 4 cut(s) 247, 290, 470, 1174
HpyAV CCTTC 7 cut(s) 205, 220, 472, 604, 916, 1036, 1072
HpyCH4III ACNGT 6 cut(s) 60, 290, 362, 373, 1121, 1162
HpyCH4IV ACGT 2 cut(s) 423, 1172
HpyCH4V TGCA 3 cut(s) 716, 755, 820
HpyF10VI GCNNNNNNNGC 2 cut(s) 175, 388
HpyF3I CTNAG 1 cut(s) 23
HpySE526I ACGT 2 cut(s) 423, 1172
Hsp92II CATG 2 cut(s) 1027, 1088
HspAI GCGC 1 cut(s) 173
KflI GGGWCCC 1 cut(s) 351
Kzo9I GATC 3 cut(s) 472, 541, 1090
LguI GCTCTTC 1 cut(s) 47
LmnI GCTCC 2 cut(s) 1030, 1126
Lsp1109I GCAGC 5 cut(s) 187, 713, 728, 747, 804
LweI GCATC 1 cut(s) 787
MaeI CTAG 4 cut(s) 383, 633, 774, 839
MaeII ACGT 2 cut(s) 423, 1172
MaeIII GTNAC 2 cut(s) 367, 457
MalI GATC 3 cut(s) 474, 543, 1092
MboI GATC 3 cut(s) 472, 541, 1090
MboII GAAGA 5 cut(s) 34, 739, 806, 836, 878
MflI RGATCY 1 cut(s) 541
MlsI TGGCCA 1 cut(s) 640
MluCI AATT 5 cut(s) 18, 720, 1050, 1066, 1102
MluNI TGGCCA 1 cut(s) 640
MlyI GAGTC 1 cut(s) 797
Mox20I TGGCCA 1 cut(s) 640
MroXI GAANNNNTTC 1 cut(s) 209
MscI TGGCCA 1 cut(s) 640
MseI TTAA 5 cut(s) 327, 743, 782, 917, 1164
MslI CAYNNNNRTG 1 cut(s) 360
Msp20I TGGCCA 1 cut(s) 640
MspI CCGG 3 cut(s) 11, 508, 538
MspR9I CCNGG 4 cut(s) 301, 509, 538, 539
Mva1269I GAATGC 1 cut(s) 447
MvaI CCWGG 1 cut(s) 301
MvnI CGCG 2 cut(s) 98, 173
MwoI GCNNNNNNNGC 2 cut(s) 175, 388
NciI CCSGG 3 cut(s) 509, 538, 539
NdeII GATC 3 cut(s) 472, 541, 1090
NlaIII CATG 2 cut(s) 1027, 1088
NlaIV GGNNCC 3 cut(s) 352, 353, 397
NmuCI GTSAC 2 cut(s) 367, 457
OliI CACNNNNGTG 1 cut(s) 360
PciSI GCTCTTC 1 cut(s) 47
PcsI WCGNNNNNNNCGW 1 cut(s) 1166
PctI GAATGC 1 cut(s) 447
PdmI GAANNNNTTC 1 cut(s) 209
PfeI GAWTC 2 cut(s) 629, 845
PflFI GACNNNGTC 1 cut(s) 58
PkrI GCNGC 9 cut(s) 177, 180, 323, 506, 703, 718, 737, 802, 819
PleI GAGTC 1 cut(s) 796
PpsI GAGTC 1 cut(s) 796
Ppu21I YACGTR 1 cut(s) 424
PpuMI RGGWCCY 2 cut(s) 351, 1059
PshAI GACNNNNGTC 1 cut(s) 464
Psp5II RGGWCCY 2 cut(s) 351, 1059
Psp6I CCWGG 1 cut(s) 299
PspGI CCWGG 1 cut(s) 299
PspN4I GGNNCC 3 cut(s) 352, 353, 397
PspPI GGNCC 5 cut(s) 351, 396, 535, 763, 1059
PspPPI RGGWCCY 2 cut(s) 351, 1059
PstI CTGCAG 2 cut(s) 718, 757
PsuI RGATCY 1 cut(s) 541
PsyI GACNNNGTC 1 cut(s) 58
RsaI GTAC 2 cut(s) 422, 434
RsaNI GTAC 2 cut(s) 421, 433
RseI CAYNNNNRTG 1 cut(s) 360
SapI GCTCTTC 1 cut(s) 47
SaqAI TTAA 5 cut(s) 327, 743, 782, 917, 1164
SatI GCNGC 9 cut(s) 176, 179, 322, 505, 702, 717, 736, 801, 818
Sau3AI GATC 3 cut(s) 472, 541, 1090
Sau96I GGNCC 5 cut(s) 351, 396, 535, 763, 1059
SchI GAGTC 1 cut(s) 797
ScrFI CCNGG 4 cut(s) 301, 509, 538, 539
SfaNI GCATC 1 cut(s) 787
SfcI CTRYAG 2 cut(s) 714, 753
SinI GGWCC 2 cut(s) 351, 1059
SmaI CCCGGG 1 cut(s) 539
SmiI ATTTAAAT 1 cut(s) 783
SmiMI CAYNNNNRTG 1 cut(s) 360
SmlI CTYRAG 1 cut(s) 767
SmoI CTYRAG 1 cut(s) 767
SpeI ACTAGT 1 cut(s) 838
Sse9I AATT 5 cut(s) 18, 720, 1050, 1066, 1102
SspMI CTAG 4 cut(s) 383, 633, 774, 839
StyD4I CCNGG 4 cut(s) 299, 507, 536, 537
SwaI ATTTAAAT 1 cut(s) 783
TaaI ACNGT 6 cut(s) 60, 290, 362, 373, 1121, 1162
TaiI ACGT 2 cut(s) 426, 1175
TaqI TCGA 3 cut(s) 130, 513, 1014
TasI AATT 5 cut(s) 18, 720, 1050, 1066, 1102
TauI GCSGC 4 cut(s) 181, 324, 507, 803
TfiI GAWTC 2 cut(s) 629, 845
Tru1I TTAA 5 cut(s) 327, 743, 782, 917, 1164
Tru9I TTAA 5 cut(s) 327, 743, 782, 917, 1164
TscAI CASTG 1 cut(s) 988
TseFI GTSAC 2 cut(s) 367, 457
TseI GCWGC 5 cut(s) 175, 701, 716, 735, 817
Tsp45I GTSAC 2 cut(s) 367, 457
TspDTI ATGAA 3 cut(s) 642, 935, 1012
TspGWI ACGGA 2 cut(s) 345, 838
TspMI CCCGGG 1 cut(s) 537
TspRI CASTG 1 cut(s) 988
Tth111I GACNNNGTC 1 cut(s) 58
VpaK11BI GGWCC 2 cut(s) 351, 1059
XapI RAATTY 2 cut(s) 18, 1102
XmaI CCCGGG 1 cut(s) 537
XmnI GAANNNNTTC 1 cut(s) 209
XspI CTAG 4 cut(s) 383, 633, 774, 839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.