Rorug02G0100600

Transmembrane protein 53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0014260
Physical Location & Seq
Forward (+)
8559241 .. 8561253
2013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0100600.1

Sequence Viewer

Length: 1002 bp
ATGAGAAGCAAGAAAGCGAAGAAACCCAAACTGGAGGAGAAAGTGTCTCAATCGCACATTGAGATTGTGAACATTGAAGACGATGAGCTTTGCTTCACACCAAAACGCTCTAGCAAGTTCAATTCTATCTCAGTCGATGAGAACAGAAACCTTCAATCAATCAAGGAATCTATTAAGGCCTCTCTTCTTAAGTCCACAGGTAATTTCATCGACCTTTCTGATGAAACTCATTCGGAAGATGACGAAGAGCTTAGAGTTCTTAGGTTTCAACCCCCAAACACTACATTCGGAAAGCGAAAGGAACCCTTTAGTAGTCCTTCTGTAACCGAAGTTGGGGAATCTTCTAATTCCAATTCCAAAAAAGACCCATCATTTTTATGTGAAATTTGCGCCGAGCCCAAGTCTAGGAGCGAGTCATTTGGCATAAAAGACTGTAGTCATTCATATTGTACTGAGTGCATGATCAAATACGTGGCATCCAAGCTTCAAGAAAATGTTACATTCATTAAGTGCCCTGTTCCTGATTGCAAAGGGTTACTGGAGCCGGAGTATTACCGTCAGATTCTGCCCCCTGAGGTGTTTGAGAGGTGGGGAAGTGCATTGTGTGAGGCGGTGATTCTCGGGTCTGAGAAGTTTTACTGCCCCTTTAAGGATTGCTCCGGAATGTTGATTGATGATGGAAAAGAGGTTGTACGGGAATCGGAGTGCCCTAATTGTTGGAGAATGTTCTGTGCGCAATGTAAGGTTCCTTGGCATGCTGGGATTGACTGCGCAGAGTTTCAGAAGTTGAATAAGGATGAGAGGGAAAAAGAAGATATTATGTTGAGAAAACTTGCACAGAACAAAGAGTGGAAGAGATGTCCCAAGTGTAGGTTCTATGTGGAAAAATCAGAAGGCTGCATGTTCATGAAGTGTAGGTGCCAAACTACTTTCTGCTACAGATGCGAACACGTTTTAAAGAGAAACCATAACCACTATTGTCCCAATTGTAAAGGGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

333

Amino Acids

38.56

Weight (kDa)

7.9

Isoelectric Point (pI)

63.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IBR PF01485 207 - 257 2e-09 IBR domain, a half RING-finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18245
fragaria_vesca FvH4_1g13010
malus_domestica MD02G1144600.v1.1 MD15G1258300.v1.1
prunus_persica Prupe.7G157000_v2.0.a1 Prupe.7G157000_v2.0.a1
pyrus_communis pycom02g11450 pycom15g22610
rosa_chinensis RchiOBHm_Chr2g0101161
rosa_laevigata RLG00000017051
rosa_roxburghii Rroxscaffold_2G00141530
rosa_rugosa Rorug02G0100600
rosa_samantha Rh2AG144000 Rh2BG149000 Rh2CG150300 Rh2DG149500
rosa_wichuraiana Rw2G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 735, 772
AccB1I GGYRCC 1 cut(s) 918
AccIII TCCGGA 1 cut(s) 659
AciI CCGC 1 cut(s) 611
AcsI RAATTY 1 cut(s) 384
AfaI GTAC 2 cut(s) 451, 693
AfiI CCNNNNNNNGG 4 cut(s) 333, 405, 649, 870
AflII CTTAAG 1 cut(s) 188
AflIII ACRYGT 1 cut(s) 949
AgsI TTSAA 6 cut(s) 77, 121, 155, 269, 488, 790
AjuI GAANNNNNNNTTGG 2 cut(s) 857, 889
AluBI AGCT 3 cut(s) 88, 250, 484
AluI AGCT 3 cut(s) 88, 250, 484
Alw26I GTCTC 1 cut(s) 51
AlwNI CAGNNNCTG 1 cut(s) 565
Ama87I CYCGRG 1 cut(s) 620
Aor13HI TCCGGA 1 cut(s) 659
AoxI GGCC 1 cut(s) 177
ApeKI GCWGC 1 cut(s) 897
ApoI RAATTY 1 cut(s) 384
AspLEI GCGC 4 cut(s) 392, 736, 773, 999
AsuHPI GGTGA 1 cut(s) 625
AvaI CYCGRG 1 cut(s) 620
AxyI CCTNAGG 1 cut(s) 573
BaeGI GKGCMC 2 cut(s) 515, 710
BanI GGYRCC 1 cut(s) 918
BanII GRGCYC 1 cut(s) 399
BbsI GAAGAC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 884
BccI CCATC 2 cut(s) 376, 671
BclI TGATCA 1 cut(s) 462
BcoDI GTCTC 1 cut(s) 51
BfaI CTAG 2 cut(s) 111, 405
BfmI CTRYAG 2 cut(s) 433, 937
BfoI RGCGCY 1 cut(s) 1000
BfrI CTTAAG 1 cut(s) 188
BisI GCNGC 1 cut(s) 898
BlsI GCNGC 1 cut(s) 899
BmeT110I CYCGRG 1 cut(s) 620
BmiI GGNNCC 4 cut(s) 303, 543, 747, 920
BmsI GCATC 2 cut(s) 485, 932
BoxI GACNNNNGTC 1 cut(s) 435
BpiI GAAGAC 1 cut(s) 84
BpmI CTGGAG 2 cut(s) 53, 560
BsaAI YACGTR 1 cut(s) 472
BsaJI CCNNGG 1 cut(s) 749
BsaWI WCCGGW 1 cut(s) 659
BsaXI ACNNNNNCTCC 4 cut(s) 29, 59, 533, 563
Bsc4I CCNNNNNNNGG 4 cut(s) 333, 405, 649, 870
Bse1I ACTGG 2 cut(s) 36, 543
Bse21I CCTNAGG 1 cut(s) 573
Bse3DI GCAATG 1 cut(s) 743
BseAI TCCGGA 1 cut(s) 659
BseDI CCNNGG 1 cut(s) 749
BseGI GGATG 2 cut(s) 476, 802
BseLI CCNNNNNNNGG 4 cut(s) 333, 405, 649, 870
BseMI GCAATG 1 cut(s) 743
BseMII CTCAG 4 cut(s) 144, 444, 564, 618
BseNI ACTGG 2 cut(s) 36, 543
BseRI GAGGAG 1 cut(s) 50
BseSI GKGCMC 2 cut(s) 515, 710
BseXI GCAGC 1 cut(s) 884
BseYI CCCAGC 1 cut(s) 758
BshFI GGCC 1 cut(s) 179
BshNI GGYRCC 1 cut(s) 918
BsiHKCI CYCGRG 1 cut(s) 620
BsiSI CCGG 2 cut(s) 545, 660
BslFI GGGAC 2 cut(s) 846, 966
BslI CCNNNNNNNGG 4 cut(s) 333, 405, 649, 870
BsmAI GTCTC 1 cut(s) 51
BsmFI GGGAC 2 cut(s) 846, 966
BsnI GGCC 1 cut(s) 179
BsoBI CYCGRG 1 cut(s) 620
Bsp1286I GDGCHC 3 cut(s) 399, 515, 710
Bsp13I TCCGGA 1 cut(s) 659
Bsp143I GATC 1 cut(s) 462
BspACI CCGC 1 cut(s) 611
BspANI GGCC 1 cut(s) 179
BspCNI CTCAG 4 cut(s) 143, 445, 565, 619
BspEI TCCGGA 1 cut(s) 659
BspHI TCATGA 1 cut(s) 906
BspLI GGNNCC 4 cut(s) 303, 543, 747, 920
BspQI GCTCTTC 1 cut(s) 240
BspT107I GGYRCC 1 cut(s) 918
BspTI CTTAAG 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 743
BsrI ACTGG 2 cut(s) 36, 543
BssECI CCNNGG 1 cut(s) 749
BssMI GATC 1 cut(s) 462
BssT1I CCWWGG 1 cut(s) 749
Bst4CI ACNGT 2 cut(s) 434, 557
Bst6I CTCTTC 3 cut(s) 189, 240, 848
BstAFI CTTAAG 1 cut(s) 188
BstBAI YACGTR 1 cut(s) 472
BstC8I GCNNGC 1 cut(s) 756
BstDEI CTNAG 6 cut(s) 130, 251, 260, 453, 573, 627
BstF5I GGATG 2 cut(s) 476, 802
BstH2I RGCGCY 1 cut(s) 1000
BstHHI GCGC 4 cut(s) 392, 736, 773, 999
BstKTI GATC 1 cut(s) 465
BstMAI GTCTC 1 cut(s) 51
BstMBI GATC 1 cut(s) 462
BstMWI GCNNNNNNNGC 1 cut(s) 942
BstNSI RCATGY 2 cut(s) 758, 904
BstPAI GACNNNNGTC 1 cut(s) 435
BstSFI CTRYAG 2 cut(s) 433, 937
BstSLI GKGCMC 2 cut(s) 515, 710
BstV1I GCAGC 1 cut(s) 884
BstV2I GAAGAC 1 cut(s) 84
Bsu36I CCTNAGG 1 cut(s) 573
BsuRI GGCC 1 cut(s) 179
BtsCI GGATG 2 cut(s) 476, 802
Cac8I GCNNGC 1 cut(s) 756
CaiI CAGNNNCTG 1 cut(s) 565
CciI TCATGA 1 cut(s) 906
CfoI GCGC 4 cut(s) 392, 736, 773, 999
Csp6I GTAC 2 cut(s) 450, 692
CviAII CATG 4 cut(s) 460, 755, 901, 907
CviJI RGCY 7 cut(s) 88, 179, 250, 397, 484, 544, 897
CviKI_1 RGCY 7 cut(s) 88, 179, 250, 397, 484, 544, 897
CviQI GTAC 2 cut(s) 450, 692
DdeI CTNAG 6 cut(s) 130, 251, 260, 453, 573, 627
DpnI GATC 1 cut(s) 464
DpnII GATC 1 cut(s) 462
DraI TTTAAA 1 cut(s) 957
Eam1104I CTCTTC 3 cut(s) 189, 240, 848
EarI CTCTTC 3 cut(s) 189, 240, 848
Eco130I CCWWGG 1 cut(s) 749
Eco147I AGGCCT 1 cut(s) 179
Eco24I GRGCYC 1 cut(s) 399
Eco81I CCTNAGG 1 cut(s) 573
Eco88I CYCGRG 1 cut(s) 620
EcoT14I CCWWGG 1 cut(s) 749
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 749
FaeI CATG 4 cut(s) 463, 758, 904, 910
FalI AAGNNNNNCTT 2 cut(s) 290, 322
FaqI GGGAC 2 cut(s) 846, 966
FatI CATG 4 cut(s) 459, 754, 900, 906
FbaI TGATCA 1 cut(s) 462
Fnu4HI GCNGC 1 cut(s) 898
FokI GGATG 2 cut(s) 463, 809
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 1 cut(s) 898
FspBI CTAG 2 cut(s) 111, 405
FspI TGCGCA 2 cut(s) 735, 772
GlaI GCGC 4 cut(s) 391, 735, 772, 998
GluI GCNGC 1 cut(s) 898
GsaI CCCAGC 1 cut(s) 762
GsuI CTGGAG 2 cut(s) 53, 560
HaeII RGCGCY 1 cut(s) 1000
HaeIII GGCC 1 cut(s) 179
HapII CCGG 2 cut(s) 545, 660
HhaI GCGC 4 cut(s) 392, 736, 773, 999
Hin1II CATG 4 cut(s) 463, 758, 904, 910
Hin6I GCGC 4 cut(s) 390, 734, 771, 997
HinP1I GCGC 4 cut(s) 390, 734, 771, 997
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 6 cut(s) 167, 338, 413, 562, 616, 698
HpaII CCGG 2 cut(s) 545, 660
HphI GGTGA 1 cut(s) 625
Hpy166II GTNNAC 2 cut(s) 70, 195
Hpy188I TCNGA 8 cut(s) 220, 235, 290, 561, 628, 703, 783, 892
Hpy188III TCNNGA 4 cut(s) 488, 521, 660, 907
Hpy8I GTNNAC 2 cut(s) 70, 195
HpyAV CCTTC 3 cut(s) 161, 327, 887
HpyCH4III ACNGT 2 cut(s) 434, 557
HpyCH4IV ACGT 2 cut(s) 471, 951
HpyCH4V TGCA 5 cut(s) 459, 528, 599, 836, 900
HpyF10VI GCNNNNNNNGC 1 cut(s) 942
HpyF3I CTNAG 6 cut(s) 130, 251, 260, 453, 573, 627
HpySE526I ACGT 2 cut(s) 471, 951
Hsp92II CATG 4 cut(s) 463, 758, 904, 910
HspAI GCGC 4 cut(s) 390, 734, 771, 997
Kpn2I TCCGGA 1 cut(s) 659
Ksp22I TGATCA 1 cut(s) 462
Kzo9I GATC 1 cut(s) 462
LguI GCTCTTC 1 cut(s) 240
LmnI GCTCC 3 cut(s) 408, 541, 662
LpnPI CCDG 9 cut(s) 17, 183, 524, 528, 534, 558, 585, 673, 744
Lsp1109I GCAGC 1 cut(s) 884
LweI GCATC 2 cut(s) 485, 932
MaeI CTAG 2 cut(s) 111, 405
MaeII ACGT 2 cut(s) 471, 951
MaeIII GTNAC 3 cut(s) 322, 496, 534
MalI GATC 1 cut(s) 464
MboI GATC 1 cut(s) 462
MboII GAAGA 8 cut(s) 31, 89, 176, 248, 257, 333, 824, 865
MfeI CAATTG 1 cut(s) 985
MhlI GDGCHC 3 cut(s) 399, 515, 710
MluCI AATT 7 cut(s) 121, 202, 346, 352, 384, 712, 985
MlyI GAGTC 1 cut(s) 422
MmeI TCCRAC 1 cut(s) 698
MnlI CCTC 7 cut(s) 28, 190, 568, 579, 601, 679, 795
MroI TCCGGA 1 cut(s) 659
MseI TTAA 5 cut(s) 174, 189, 507, 648, 956
MslI CAYNNNNRTG 2 cut(s) 376, 905
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 2 cut(s) 545, 660
MunI CAATTG 1 cut(s) 985
MwoI GCNNNNNNNGC 1 cut(s) 942
NdeII GATC 1 cut(s) 462
NlaIII CATG 4 cut(s) 463, 758, 904, 910
NlaIV GGNNCC 4 cut(s) 303, 543, 747, 920
NmeAIII GCCGAG 1 cut(s) 418
NsbI TGCGCA 2 cut(s) 735, 772
NspI RCATGY 2 cut(s) 758, 904
PaeI GCATGC 1 cut(s) 758
PagI TCATGA 1 cut(s) 906
PceI AGGCCT 1 cut(s) 179
PciSI GCTCTTC 1 cut(s) 240
PfeI GAWTC 5 cut(s) 167, 338, 562, 616, 698
PkrI GCNGC 1 cut(s) 899
PleI GAGTC 1 cut(s) 421
PpsI GAGTC 1 cut(s) 421
Ppu21I YACGTR 1 cut(s) 472
PshAI GACNNNNGTC 1 cut(s) 435
PspFI CCCAGC 1 cut(s) 758
PspN4I GGNNCC 4 cut(s) 303, 543, 747, 920
PstNI CAGNNNCTG 1 cut(s) 565
RsaI GTAC 2 cut(s) 451, 693
RsaNI GTAC 2 cut(s) 450, 692
RseI CAYNNNNRTG 2 cut(s) 376, 905
SapI GCTCTTC 1 cut(s) 240
SaqAI TTAA 5 cut(s) 174, 189, 507, 648, 956
SatI GCNGC 1 cut(s) 898
Sau3AI GATC 1 cut(s) 462
SchI GAGTC 1 cut(s) 422
SduI GDGCHC 3 cut(s) 399, 515, 710
SfaNI GCATC 2 cut(s) 485, 932
SfcI CTRYAG 2 cut(s) 433, 937
SmiMI CAYNNNNRTG 2 cut(s) 376, 905
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
SphI GCATGC 1 cut(s) 758
Sse9I AATT 7 cut(s) 121, 202, 346, 352, 384, 712, 985
SseBI AGGCCT 1 cut(s) 179
SsiI CCGC 1 cut(s) 611
SspMI CTAG 2 cut(s) 111, 405
StuI AGGCCT 1 cut(s) 179
StyI CCWWGG 1 cut(s) 749
TaaI ACNGT 2 cut(s) 434, 557
TaiI ACGT 2 cut(s) 474, 954
TaqI TCGA 2 cut(s) 135, 210
TasI AATT 7 cut(s) 121, 202, 346, 352, 384, 712, 985
TatI WGTACW 1 cut(s) 449
TfiI GAWTC 5 cut(s) 167, 338, 562, 616, 698
Tru1I TTAA 5 cut(s) 174, 189, 507, 648, 956
Tru9I TTAA 5 cut(s) 174, 189, 507, 648, 956
TseI GCWGC 1 cut(s) 897
TspDTI ATGAA 6 cut(s) 196, 237, 432, 493, 895, 923
Vha464I CTTAAG 1 cut(s) 188
XapI RAATTY 1 cut(s) 384
XceI RCATGY 2 cut(s) 758, 904
XspI CTAG 2 cut(s) 111, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.