Rh2DG149500

Transmembrane protein 53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014260
Physical Location & Seq
Forward (+)
12498353 .. 12500209
1857 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG149500.1

Sequence Viewer

Length: 1182 bp
ATGGAGACTCCACTGAGAATCCTCAGCCTCAACCGCCACCTCAAAACGACGCCGTCCGCTCTTTTCTTCTCCCCCAAATTTCACCCACCTCAAAAACCCTCACTTGCATTTCCCAATTATTTCCGGCTAAACGACGCCGTAAAGAGAACCCCTTTTGCTCTTTCTCACTCCTCCTCTTCCTGGAATCCAAACCCCTTCAAATTCAACCCCTCAATTTCCCAATCTCCATTTCCGATCACCAATTTCAACCACTCCCTGTCGTACCAAAACGACGCCGGTTTCAGATGGAACCGAGCGGTCCACAGCGGCATTGACGGAAATGTGGGATCTTTGGGGGCCCAAAAGCGGGAAGTGACGGTGGTGTTGCTGGGCTGGCTTGGGGGTCAGACCAAGCACATGAAGAGGTACGTGGAGTGGTACAATTCCCGAGGCTTTCACGCTGTGACTTTTGTTGTGGACGTCAAGGAGGTGCTCTGGTTTGATTTGGGCCACCGGGTTGAGAAGCGGGTCGCGGAGCTGGCTCATGAGCTGGCTTCGTGGGTTGAGGGCGGCGGAGAACGGTGTTTGGTGTTTCACACTTTTAGCAATACGGGTTGGTTTGTATATGGTGCAATACTTGAAATTTGGCTGGATAGACCAGACTTGATCAAGAAGATTAAAGGGTGTATTGTTGATTCAGGAGCTGGAGCCCCTTTTGATCCTAAGGTGTGGGCAGCAGGATTCTCTACTGCGATTATGAAGAAACACAGCTCTTTAGCAAAACCTGCGGTTGAGACTAAAGAATTAAATGCCTCTGGAGGTGCAGCGAGCTTGTCAAAGGTGCAAGAAGAGGAACCTCCAGCAATTGAAACCATGCTTCTGTTGGTATTAGAGAAGCTGTTCTCACTCATTCTGAAGTTGCCTGATGTGGATAAGAGGTTAACCAAGGTAGTTTCTGTCCTCTCGACAAACCCTCCTCATTGTCCACAGCTTTACCTGTACAGTACGGGTGATAAAGTCGTCCCACACCAGTCCATCGAATCCTTCATCGCAGAGCAAAGGCAGATAGGTAGACCCGTGAAGTCCTTCAATTTCGGCACATCTCCTCACGTCGACCATTACAGGACTTTCCCCAACATATACTCTCAGGAGCTTAACAATTTCTTGAAAGAATGCTTGGCTACAACAGTTAAGCAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

393

Amino Acids

44.13

Weight (kDa)

9.32

Isoelectric Point (pI)

42.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 119 - 382 1.1e-62 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18245
fragaria_vesca FvH4_1g13010
malus_domestica MD02G1144600.v1.1 MD15G1258300.v1.1
prunus_persica Prupe.7G157000_v2.0.a1 Prupe.7G157000_v2.0.a1
pyrus_communis pycom02g11450 pycom15g22610
rosa_chinensis RchiOBHm_Chr2g0101161
rosa_laevigata RLG00000017051
rosa_roxburghii Rroxscaffold_2G00141530
rosa_rugosa Rorug02G0100600
rosa_samantha Rh2AG144000 Rh2BG149000 Rh2CG150300 Rh2DG149500
rosa_wichuraiana Rw2G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 462
Acc36I ACCTGC 1 cut(s) 772
AccBSI CCGCTC 2 cut(s) 59, 296
AccI GTMKAC 2 cut(s) 1051, 1092
AccII CGCG 1 cut(s) 512
AclWI GGATC 2 cut(s) 334, 692
AcsI RAATTY 3 cut(s) 77, 200, 621
AcuI CTGAAG 1 cut(s) 914
AcyI GRCGYC 4 cut(s) 50, 135, 273, 459
AdeI CACNNNGTG 1 cut(s) 442
AfaI GTAC 5 cut(s) 263, 407, 419, 980, 985
AfiI CCNNNNNNNGG 3 cut(s) 180, 345, 346
AgsI TTSAA 7 cut(s) 199, 205, 247, 620, 848, 1069, 1147
AjiI CACGTC 1 cut(s) 1090
AjnI CCWGG 1 cut(s) 179
AjuI GAANNNNNNNTTGG 4 cut(s) 213, 245, 1139, 1171
AluBI AGCT 8 cut(s) 517, 529, 683, 750, 810, 877, 970, 1132
AluI AGCT 8 cut(s) 517, 529, 683, 750, 810, 877, 970, 1132
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 1 cut(s) 767
AlwI GGATC 2 cut(s) 334, 692
AlwNI CAGNNNCTG 1 cut(s) 683
Ama87I CYCGRG 1 cut(s) 426
AoxI GGCC 2 cut(s) 336, 487
ApaI GGGCCC 1 cut(s) 340
ApeKI GCWGC 2 cut(s) 713, 803
ApoI RAATTY 3 cut(s) 77, 200, 621
Asp700I GAANNNNTTC 2 cut(s) 878, 1064
AspS9I GGNCC 4 cut(s) 298, 336, 337, 487
AsuC2I CCSGG 1 cut(s) 494
AsuHPI GGTGA 3 cut(s) 74, 229, 1001
AvaI CYCGRG 1 cut(s) 426
AvaII GGWCC 1 cut(s) 298
AxyI CCTNAGG 1 cut(s) 702
BaeGI GKGCMC 1 cut(s) 340
BanII GRGCYC 2 cut(s) 340, 691
Bbv12I GWGCWC 1 cut(s) 474
BbvCI CCTCAGC 1 cut(s) 23
BbvI GCAGC 2 cut(s) 725, 815
BccI CCATC 2 cut(s) 279, 1022
BceAI ACGGC 2 cut(s) 37, 122
BciT130I CCWGG 1 cut(s) 181
BclI TGATCA 1 cut(s) 645
BcnI CCSGG 1 cut(s) 494
BcoDI GTCTC 1 cut(s) 767
BfuAI ACCTGC 1 cut(s) 772
BisI GCNGC 4 cut(s) 307, 550, 714, 804
BlsI GCNGC 4 cut(s) 308, 551, 715, 805
Bme1390I CCNGG 2 cut(s) 181, 494
Bme18I GGWCC 1 cut(s) 298
BmeT110I CYCGRG 1 cut(s) 426
BmgBI CACGTC 1 cut(s) 1090
BmgT120I GGNCC 4 cut(s) 298, 336, 337, 487
BmiI GGNNCC 5 cut(s) 290, 337, 338, 688, 834
BmrFI CCNGG 2 cut(s) 181, 494
BpmI CTGGAG 3 cut(s) 705, 816, 822
Bpu10I CCTNAGC 1 cut(s) 23
BpuMI CCSGG 1 cut(s) 494
BsaAI YACGTR 1 cut(s) 409
BsaHI GRCGYC 4 cut(s) 50, 135, 273, 459
BsaJI CCNNGG 2 cut(s) 427, 924
BsaXI ACNNNNNCTCC 2 cut(s) 937, 967
Bsc4I CCNNNNNNNGG 3 cut(s) 180, 345, 346
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 1 cut(s) 1009
Bse21I CCTNAGG 1 cut(s) 702
BseBI CCWGG 1 cut(s) 181
BseDI CCNNGG 2 cut(s) 427, 924
BseLI CCNNNNNNNGG 3 cut(s) 180, 345, 346
BseMII CTCAG 3 cut(s) 5, 37, 1139
BseNI ACTGG 1 cut(s) 1009
BseRI GAGGAG 4 cut(s) 160, 163, 945, 1074
BseSI GKGCMC 1 cut(s) 340
BseXI GCAGC 2 cut(s) 725, 815
BseYI CCCAGC 1 cut(s) 367
BsgI GTGCAG 1 cut(s) 822
Bsh1236I CGCG 1 cut(s) 512
BshFI GGCC 2 cut(s) 338, 489
BsiHKAI GWGCWC 1 cut(s) 474
BsiHKCI CYCGRG 1 cut(s) 426
BsiSI CCGG 3 cut(s) 124, 276, 493
BslFI GGGAC 1 cut(s) 986
BslI CCNNNNNNNGG 3 cut(s) 180, 345, 346
BsmAI GTCTC 1 cut(s) 767
BsmFI GGGAC 1 cut(s) 986
BsmI GAATGC 1 cut(s) 1157
BsnI GGCC 2 cut(s) 338, 489
BsoBI CYCGRG 1 cut(s) 426
Bsp120I GGGCCC 1 cut(s) 336
Bsp1286I GDGCHC 3 cut(s) 340, 474, 691
Bsp1407I TGTACA 1 cut(s) 978
Bsp143I GATC 4 cut(s) 234, 326, 645, 697
BspANI GGCC 2 cut(s) 338, 489
BspCNI CTCAG 3 cut(s) 6, 36, 1138
BspFNI CGCG 1 cut(s) 512
BspHI TCATGA 1 cut(s) 523
BspLI GGNNCC 5 cut(s) 290, 337, 338, 688, 834
BspMI ACCTGC 1 cut(s) 772
BspPI GGATC 2 cut(s) 334, 692
BsrBI CCGCTC 2 cut(s) 59, 296
BsrFI RCCGGY 1 cut(s) 275
BsrGI TGTACA 1 cut(s) 978
BsrI ACTGG 1 cut(s) 1009
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 2 cut(s) 427, 924
BssMI GATC 4 cut(s) 234, 326, 645, 697
BssNI GRCGYC 4 cut(s) 50, 135, 273, 459
BssT1I CCWWGG 1 cut(s) 924
Bst2UI CCWGG 1 cut(s) 181
Bst4CI ACNGT 4 cut(s) 358, 561, 983, 1168
Bst6I CTCTTC 3 cut(s) 181, 395, 822
BstACI GRCGYC 4 cut(s) 50, 135, 273, 459
BstAPI GCANNNNNTGC 1 cut(s) 764
BstAUI TGTACA 1 cut(s) 978
BstBAI YACGTR 1 cut(s) 409
BstC8I GCNNGC 4 cut(s) 374, 519, 531, 808
BstDEI CTNAG 4 cut(s) 14, 23, 702, 1125
BstFNI CGCG 1 cut(s) 512
BstKTI GATC 4 cut(s) 237, 329, 648, 700
BstMAI GTCTC 1 cut(s) 767
BstMBI GATC 4 cut(s) 234, 326, 645, 697
BstMWI GCNNNNNNNGC 4 cut(s) 33, 373, 518, 764
BstNI CCWGG 1 cut(s) 181
BstSCI CCNGG 2 cut(s) 179, 492
BstSLI GKGCMC 1 cut(s) 340
BstUI CGCG 1 cut(s) 512
BstV1I GCAGC 2 cut(s) 725, 815
BstX2I RGATCY 1 cut(s) 326
BstYI RGATCY 1 cut(s) 326
Bsu36I CCTNAGG 1 cut(s) 702
BsuRI GGCC 2 cut(s) 338, 489
BtgZI GCGATG 1 cut(s) 1012
BtrI CACGTC 1 cut(s) 1090
BtsIMutI CAGTG 1 cut(s) 11
BveI ACCTGC 1 cut(s) 772
Cac8I GCNNGC 4 cut(s) 374, 519, 531, 808
CaiI CAGNNNCTG 1 cut(s) 683
CciI TCATGA 1 cut(s) 523
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 4 cut(s) 298, 336, 337, 487
CseI GACGC 3 cut(s) 58, 143, 281
Csp6I GTAC 5 cut(s) 262, 406, 418, 979, 984
CviAII CATG 3 cut(s) 397, 524, 853
CviQI GTAC 5 cut(s) 262, 406, 418, 979, 984
DdeI CTNAG 4 cut(s) 14, 23, 702, 1125
DpnI GATC 4 cut(s) 236, 328, 647, 699
DpnII GATC 4 cut(s) 234, 326, 645, 697
DraIII CACNNNGTG 1 cut(s) 442
Eam1104I CTCTTC 3 cut(s) 181, 395, 822
EarI CTCTTC 3 cut(s) 181, 395, 822
EciI GGCGGA 1 cut(s) 567
Eco130I CCWWGG 1 cut(s) 924
Eco24I GRGCYC 2 cut(s) 340, 691
Eco47I GGWCC 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 914
Eco81I CCTNAGG 1 cut(s) 702
Eco88I CYCGRG 1 cut(s) 426
EcoO109I RGGNCCY 1 cut(s) 336
EcoRII CCWGG 1 cut(s) 179
EcoT14I CCWWGG 1 cut(s) 924
EcoT38I GRGCYC 2 cut(s) 340, 691
ErhI CCWWGG 1 cut(s) 924
FaeI CATG 3 cut(s) 400, 527, 856
FaiI YATR 8 cut(s) 398, 525, 604, 606, 737, 854, 1118, 1120
FaqI GGGAC 1 cut(s) 986
FatI CATG 3 cut(s) 396, 523, 852
FauI CCCGC 2 cut(s) 339, 498
FbaI TGATCA 1 cut(s) 645
FblI GTMKAC 2 cut(s) 1051, 1092
Fnu4HI GCNGC 4 cut(s) 307, 550, 714, 804
FriOI GRGCYC 2 cut(s) 340, 691
Fsp4HI GCNGC 4 cut(s) 307, 550, 714, 804
GluI GCNGC 4 cut(s) 307, 550, 714, 804
GsaI CCCAGC 1 cut(s) 371
GsuI CTGGAG 3 cut(s) 705, 816, 822
HaeIII GGCC 2 cut(s) 338, 489
HapII CCGG 3 cut(s) 124, 276, 493
HgaI GACGC 3 cut(s) 58, 143, 281
Hin1I GRCGYC 4 cut(s) 50, 135, 273, 459
Hin1II CATG 3 cut(s) 400, 527, 856
HincII GTYRAC 2 cut(s) 921, 1093
HindII GTYRAC 2 cut(s) 921, 1093
HinfI GANTC 6 cut(s) 7, 18, 184, 674, 720, 1019
HpaI GTTAAC 1 cut(s) 921
HpaII CCGG 3 cut(s) 124, 276, 493
HphI GGTGA 3 cut(s) 74, 229, 1001
Hpy166II GTNNAC 6 cut(s) 301, 457, 921, 965, 1052, 1093
Hpy188I TCNGA 4 cut(s) 234, 284, 387, 894
Hpy188III TCNNGA 8 cut(s) 426, 524, 649, 678, 795, 943, 1127, 1144
Hpy8I GTNNAC 6 cut(s) 301, 457, 921, 965, 1052, 1093
Hpy99I CGWCG 4 cut(s) 52, 137, 275, 1094
HpyAV CCTTC 3 cut(s) 205, 1033, 1075
HpyCH4III ACNGT 4 cut(s) 358, 561, 983, 1168
HpyCH4IV ACGT 3 cut(s) 408, 459, 1089
HpyCH4V TGCA 4 cut(s) 107, 611, 803, 823
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 373, 518, 764
HpyF3I CTNAG 4 cut(s) 14, 23, 702, 1125
HpySE526I ACGT 3 cut(s) 408, 459, 1089
Hsp92I GRCGYC 4 cut(s) 50, 135, 273, 459
Hsp92II CATG 3 cut(s) 400, 527, 856
Ksp22I TGATCA 1 cut(s) 645
KspAI GTTAAC 1 cut(s) 921
Kzo9I GATC 4 cut(s) 234, 326, 645, 697
LmnI GCTCC 4 cut(s) 514, 680, 686, 1129
Lsp1109I GCAGC 2 cut(s) 725, 815
MaeII ACGT 3 cut(s) 408, 459, 1089
MaeIII GTNAC 2 cut(s) 352, 442
MalI GATC 4 cut(s) 236, 328, 647, 699
MbiI CCGCTC 2 cut(s) 59, 296
MboI GATC 4 cut(s) 234, 326, 645, 697
MboII GAAGA 6 cut(s) 58, 168, 412, 664, 751, 839
MfeI CAATTG 1 cut(s) 843
MflI RGATCY 1 cut(s) 326
MhlI GDGCHC 3 cut(s) 340, 474, 691
MroXI GAANNNNTTC 2 cut(s) 878, 1064
MseI TTAA 5 cut(s) 657, 785, 920, 1134, 1170
MspA1I CMGCKG 1 cut(s) 306
MspI CCGG 3 cut(s) 124, 276, 493
MspR9I CCNGG 2 cut(s) 181, 494
MunI CAATTG 1 cut(s) 843
Mva1269I GAATGC 1 cut(s) 1157
MvaI CCWGG 1 cut(s) 181
MvnI CGCG 1 cut(s) 512
MwoI GCNNNNNNNGC 4 cut(s) 33, 373, 518, 764
NciI CCSGG 1 cut(s) 494
NdeII GATC 4 cut(s) 234, 326, 645, 697
NlaIII CATG 3 cut(s) 400, 527, 856
NlaIV GGNNCC 5 cut(s) 290, 337, 338, 688, 834
NmuCI GTSAC 2 cut(s) 352, 442
PagI TCATGA 1 cut(s) 523
PctI GAATGC 1 cut(s) 1157
PdmI GAANNNNTTC 2 cut(s) 878, 1064
PfeI GAWTC 5 cut(s) 18, 184, 674, 720, 1019
PflFI GACNNNGTC 1 cut(s) 52
PfoI TCCNGGA 1 cut(s) 179
PkrI GCNGC 4 cut(s) 308, 551, 715, 805
Ppu21I YACGTR 1 cut(s) 409
Psp6I CCWGG 1 cut(s) 179
PspFI CCCAGC 1 cut(s) 367
PspGI CCWGG 1 cut(s) 179
PspN4I GGNNCC 5 cut(s) 290, 337, 338, 688, 834
PspOMI GGGCCC 1 cut(s) 336
PspPI GGNCC 4 cut(s) 298, 336, 337, 487
PstNI CAGNNNCTG 1 cut(s) 683
PsuI RGATCY 1 cut(s) 326
PsyI GACNNNGTC 1 cut(s) 52
RsaI GTAC 5 cut(s) 263, 407, 419, 980, 985
RsaNI GTAC 5 cut(s) 262, 406, 418, 979, 984
SalI GTCGAC 1 cut(s) 1091
SaqAI TTAA 5 cut(s) 657, 785, 920, 1134, 1170
SatI GCNGC 4 cut(s) 307, 550, 714, 804
Sau3AI GATC 4 cut(s) 234, 326, 645, 697
Sau96I GGNCC 4 cut(s) 298, 336, 337, 487
ScrFI CCNGG 2 cut(s) 181, 494
SduI GDGCHC 3 cut(s) 340, 474, 691
SinI GGWCC 1 cut(s) 298
StyD4I CCNGG 2 cut(s) 179, 492
StyI CCWWGG 1 cut(s) 924
TaaI ACNGT 4 cut(s) 358, 561, 983, 1168
TaiI ACGT 3 cut(s) 411, 462, 1092
TaqI TCGA 3 cut(s) 944, 1017, 1092
TatI WGTACW 1 cut(s) 978
TauI GCSGC 2 cut(s) 309, 552
TfiI GAWTC 5 cut(s) 18, 184, 674, 720, 1019
Tru1I TTAA 5 cut(s) 657, 785, 920, 1134, 1170
Tru9I TTAA 5 cut(s) 657, 785, 920, 1134, 1170
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 2 cut(s) 352, 442
TseI GCWGC 2 cut(s) 713, 803
Tsp45I GTSAC 2 cut(s) 352, 442
TspDTI ATGAA 3 cut(s) 413, 752, 1015
TspGWI ACGGA 1 cut(s) 330
TspRI CASTG 1 cut(s) 18
Tth111I GACNNNGTC 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 298
XapI RAATTY 3 cut(s) 77, 200, 621
XcmI CCANNNNNNNNNTGG 1 cut(s) 859
XmiI GTMKAC 2 cut(s) 1051, 1092
XmnI GAANNNNTTC 2 cut(s) 878, 1064
ZraI GACGTC 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.