Rroxscaffold_2G00141530

Transmembrane protein 53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014260
Physical Location & Seq
Reverse (-)
79776528 .. 79780861
4334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00141530.1

Sequence Viewer

Length: 1209 bp
ATGGAAACAACCTCACTGAGAATTCTCGGCCTCAACCGCCACCTCAAAACGACGCCGTCCGCTCTTTTTCTCTCCCCCAAATTTCACCGACCTCAAAAACCGTCACTTGCATCTCCCAATTTCCGGCAAAACGACGCCGTGAAGAGAACCCCTTTTGCCCTTTCTCTCTCACATTCTCACTCCTCCTTCTCTTCCTGGAATCCAAACCCTTTCAAATTCAACCCCTCAATTTCCCAATCTCCATTTCCGATCACTAATTTCTACTACTCTCTGTCGAACCAAAACGACGCCGGTTTCAGATGGAACCGAGCGTTCCAGAGCGGCATTGACGGATATGTGGGGTCTTTGGGGGCCCAAAAGCGGGAAGTGACGGTGGTGCTGCTGGGCTGGCTTGGGGGTCAGACCAAGCACCTGAAGAGGTACGTGGAGTGGTACAATTCCCGAGGCTTTCACGCTGTGACGTTTGTTGTGGACGTCAAGGAGGTGCTCTGGTTTGATTTGGGCCACCGGGTTGAGAAGCGGGTCGCGGAGCTGGCCCATGAGCTGGCTTCGTGGGTCTCGGAGCGGGTTGAGGGCGGCGGAGAACGGTGTTTGGTGTTTCACACTTTTAGTAACACGGGTTGGTTTGTATATGGTGCAATACTTGAAATTTGGCAGGATAGACCAGACTTGATCAAGAAGATTAAAGGGTGTATTGTTGATTCAGGAGCAGGAGCCCCTTTTGATCCTAAGGTTTGGGCAGCAGGATTCTCTACTGCAATTATGAAGAAACGCAGCTCTTTAGCAAAACCTGCGGTTGAGACTAAAGAATTAAATGCCTCTGGAGGAGCAGCGAGCTTGTCAAAGGTGCAAGAAGAGGAACCTCCAGCAATTGAAACCATGCTTCTGTTGGTATTAGAGAAGCTGTTCTCACTCATTCTGAAGTTGCCTGATGTGGATAAGAGGTTAACCAAGGTAGTTTCTGTCCTCTCAACAAACCCTCCTCATTGTCCACAGCTTTACCTGTACAGTACGGGTGATAAAGTCGTCCCACACCAGTCCATCGAATCCTTCATCGCAGAGCAAAGGCAGATAGGTAGACCCGTGAAGTCCTTCAATTTCGGCACATCTCCTCACGTCGACCATTACAGGACTTTTCCTAACATATACTCTCAGGAGCTTAACAATTTCTTGAAAGAATGCTTGGCTACTACAGTGAAGCAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

402

Amino Acids

45.18

Weight (kDa)

9.44

Isoelectric Point (pI)

46.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 124 - 391 4e-64 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18245
fragaria_vesca FvH4_1g13010
malus_domestica MD02G1144600.v1.1 MD15G1258300.v1.1
prunus_persica Prupe.7G157000_v2.0.a1 Prupe.7G157000_v2.0.a1
pyrus_communis pycom02g11450 pycom15g22610
rosa_chinensis RchiOBHm_Chr2g0101161
rosa_laevigata RLG00000017051
rosa_roxburghii Rroxscaffold_2G00141530
rosa_rugosa Rorug02G0100600
rosa_samantha Rh2AG144000 Rh2BG149000 Rh2CG150300 Rh2DG149500
rosa_wichuraiana Rw2G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 477
Acc36I ACCTGC 1 cut(s) 799
AccB7I CCANNNNNTGG 1 cut(s) 544
AccBSI CCGCTC 3 cut(s) 62, 321, 565
AccI GTMKAC 2 cut(s) 1078, 1119
AccII CGCG 1 cut(s) 527
AclWI GGATC 1 cut(s) 719
AcsI RAATTY 4 cut(s) 21, 80, 215, 648
AcuI CTGAAG 2 cut(s) 434, 941
AcyI GRCGYC 4 cut(s) 53, 135, 288, 474
AdeI CACNNNGTG 1 cut(s) 457
AfaI GTAC 4 cut(s) 422, 434, 1007, 1012
AfiI CCNNNNNNNGG 4 cut(s) 123, 360, 361, 544
AgsI TTSAA 6 cut(s) 214, 220, 647, 875, 1096, 1174
AjiI CACGTC 1 cut(s) 1117
AjnI CCWGG 1 cut(s) 194
AjuI GAANNNNNNNTTGG 4 cut(s) 228, 260, 1166, 1198
AluBI AGCT 7 cut(s) 532, 544, 777, 837, 904, 997, 1159
AluI AGCT 7 cut(s) 532, 544, 777, 837, 904, 997, 1159
Alw21I GWGCWC 1 cut(s) 489
Alw26I GTCTC 2 cut(s) 562, 794
AlwI GGATC 1 cut(s) 719
Ama87I CYCGRG 1 cut(s) 441
AoxI GGCC 4 cut(s) 28, 351, 502, 534
ApaI GGGCCC 1 cut(s) 355
ApeKI GCWGC 4 cut(s) 379, 740, 774, 830
ApoI RAATTY 4 cut(s) 21, 80, 215, 648
Asp700I GAANNNNTTC 2 cut(s) 905, 1091
AspS9I GGNCC 4 cut(s) 351, 352, 502, 535
AsuC2I CCSGG 1 cut(s) 509
AsuHPI GGTGA 2 cut(s) 77, 1028
AvaI CYCGRG 1 cut(s) 441
AxyI CCTNAGG 1 cut(s) 729
BaeGI GKGCMC 1 cut(s) 355
BanII GRGCYC 2 cut(s) 355, 718
Bbv12I GWGCWC 1 cut(s) 489
BbvI GCAGC 4 cut(s) 366, 752, 786, 842
BccI CCATC 2 cut(s) 294, 1049
BceAI ACGGC 2 cut(s) 40, 122
BciT130I CCWGG 1 cut(s) 196
BclI TGATCA 1 cut(s) 672
BcnI CCSGG 1 cut(s) 509
BcoDI GTCTC 2 cut(s) 562, 794
BfmI CTRYAG 1 cut(s) 1191
BfuAI ACCTGC 1 cut(s) 799
BisI GCNGC 6 cut(s) 322, 380, 577, 741, 775, 831
BlsI GCNGC 6 cut(s) 323, 381, 578, 742, 776, 832
Bme1390I CCNGG 2 cut(s) 196, 509
BmeT110I CYCGRG 1 cut(s) 441
BmgBI CACGTC 1 cut(s) 1117
BmgT120I GGNCC 4 cut(s) 351, 352, 502, 535
BmiI GGNNCC 5 cut(s) 305, 352, 353, 715, 861
BmrFI CCNGG 2 cut(s) 196, 509
BmsI GCATC 1 cut(s) 119
BpmI CTGGAG 2 cut(s) 843, 849
BpuMI CCSGG 1 cut(s) 509
BsaAI YACGTR 1 cut(s) 424
BsaHI GRCGYC 4 cut(s) 53, 135, 288, 474
BsaI GGTCTC 1 cut(s) 562
BsaJI CCNNGG 2 cut(s) 442, 951
BsaXI ACNNNNNCTCC 2 cut(s) 964, 994
Bsc4I CCNNNNNNNGG 4 cut(s) 123, 360, 361, 544
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 1036
Bse21I CCTNAGG 1 cut(s) 729
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 2 cut(s) 442, 951
BseLI CCNNNNNNNGG 4 cut(s) 123, 360, 361, 544
BseMII CTCAG 2 cut(s) 8, 1166
BseNI ACTGG 1 cut(s) 1036
BseRI GAGGAG 4 cut(s) 172, 840, 972, 1101
BseSI GKGCMC 1 cut(s) 355
BseXI GCAGC 4 cut(s) 366, 752, 786, 842
BseYI CCCAGC 1 cut(s) 382
Bsh1236I CGCG 1 cut(s) 527
BshFI GGCC 4 cut(s) 30, 353, 504, 536
BsiHKAI GWGCWC 1 cut(s) 489
BsiHKCI CYCGRG 1 cut(s) 441
BsiSI CCGG 3 cut(s) 124, 291, 508
BslFI GGGAC 1 cut(s) 1013
BslI CCNNNNNNNGG 4 cut(s) 123, 360, 361, 544
BsmAI GTCTC 2 cut(s) 562, 794
BsmFI GGGAC 1 cut(s) 1013
BsmI GAATGC 1 cut(s) 1184
BsnI GGCC 4 cut(s) 30, 353, 504, 536
Bso31I GGTCTC 1 cut(s) 562
BsoBI CYCGRG 1 cut(s) 441
Bsp120I GGGCCC 1 cut(s) 351
Bsp1286I GDGCHC 3 cut(s) 355, 489, 718
Bsp1407I TGTACA 1 cut(s) 1005
Bsp143I GATC 3 cut(s) 249, 672, 724
BspANI GGCC 4 cut(s) 30, 353, 504, 536
BspCNI CTCAG 2 cut(s) 9, 1165
BspFNI CGCG 1 cut(s) 527
BspLI GGNNCC 5 cut(s) 305, 352, 353, 715, 861
BspMI ACCTGC 1 cut(s) 799
BspPI GGATC 1 cut(s) 719
BspTNI GGTCTC 1 cut(s) 562
BsrBI CCGCTC 3 cut(s) 62, 321, 565
BsrFI RCCGGY 1 cut(s) 290
BsrGI TGTACA 1 cut(s) 1005
BsrI ACTGG 1 cut(s) 1036
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 2 cut(s) 442, 951
BssMI GATC 3 cut(s) 249, 672, 724
BssNI GRCGYC 4 cut(s) 53, 135, 288, 474
BssT1I CCWWGG 1 cut(s) 951
Bst2UI CCWGG 1 cut(s) 196
Bst4CI ACNGT 5 cut(s) 102, 373, 588, 1010, 1195
Bst6I CTCTTC 4 cut(s) 137, 196, 410, 849
BstACI GRCGYC 4 cut(s) 53, 135, 288, 474
BstAPI GCANNNNNTGC 1 cut(s) 791
BstAUI TGTACA 1 cut(s) 1005
BstBAI YACGTR 1 cut(s) 424
BstC8I GCNNGC 4 cut(s) 389, 534, 546, 835
BstDEI CTNAG 3 cut(s) 17, 729, 1152
BstFNI CGCG 1 cut(s) 527
BstKTI GATC 3 cut(s) 252, 675, 727
BstMAI GTCTC 2 cut(s) 562, 794
BstMBI GATC 3 cut(s) 249, 672, 724
BstMWI GCNNNNNNNGC 4 cut(s) 36, 388, 533, 791
BstNI CCWGG 1 cut(s) 196
BstSCI CCNGG 2 cut(s) 194, 507
BstSFI CTRYAG 1 cut(s) 1191
BstSLI GKGCMC 1 cut(s) 355
BstUI CGCG 1 cut(s) 527
BstV1I GCAGC 4 cut(s) 366, 752, 786, 842
Bsu36I CCTNAGG 1 cut(s) 729
BsuRI GGCC 4 cut(s) 30, 353, 504, 536
BtgZI GCGATG 1 cut(s) 1039
BtrI CACGTC 1 cut(s) 1117
BtsIMutI CAGTG 2 cut(s) 14, 1200
BveI ACCTGC 1 cut(s) 799
Cac8I GCNNGC 4 cut(s) 389, 534, 546, 835
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 4 cut(s) 351, 352, 502, 535
CseI GACGC 3 cut(s) 61, 143, 296
Csp6I GTAC 4 cut(s) 421, 433, 1006, 1011
CviAII CATG 2 cut(s) 539, 880
CviQI GTAC 4 cut(s) 421, 433, 1006, 1011
DdeI CTNAG 3 cut(s) 17, 729, 1152
DpnI GATC 3 cut(s) 251, 674, 726
DpnII GATC 3 cut(s) 249, 672, 724
DraIII CACNNNGTG 1 cut(s) 457
Eam1104I CTCTTC 4 cut(s) 137, 196, 410, 849
EarI CTCTTC 4 cut(s) 137, 196, 410, 849
EciI GGCGGA 1 cut(s) 594
Eco130I CCWWGG 1 cut(s) 951
Eco24I GRGCYC 2 cut(s) 355, 718
Eco31I GGTCTC 1 cut(s) 562
Eco57I CTGAAG 2 cut(s) 434, 941
Eco81I CCTNAGG 1 cut(s) 729
Eco88I CYCGRG 1 cut(s) 441
EcoO109I RGGNCCY 1 cut(s) 351
EcoRI GAATTC 1 cut(s) 21
EcoRII CCWGG 1 cut(s) 194
EcoT14I CCWWGG 1 cut(s) 951
EcoT38I GRGCYC 2 cut(s) 355, 718
ErhI CCWWGG 1 cut(s) 951
FaeI CATG 2 cut(s) 542, 883
FaiI YATR 8 cut(s) 336, 540, 631, 633, 764, 881, 1145, 1147
FaqI GGGAC 1 cut(s) 1013
FatI CATG 2 cut(s) 538, 879
FauI CCCGC 3 cut(s) 354, 513, 558
FbaI TGATCA 1 cut(s) 672
FblI GTMKAC 2 cut(s) 1078, 1119
Fnu4HI GCNGC 6 cut(s) 322, 380, 577, 741, 775, 831
FriOI GRGCYC 2 cut(s) 355, 718
Fsp4HI GCNGC 6 cut(s) 322, 380, 577, 741, 775, 831
GluI GCNGC 6 cut(s) 322, 380, 577, 741, 775, 831
GsaI CCCAGC 1 cut(s) 386
GsuI CTGGAG 2 cut(s) 843, 849
HaeIII GGCC 4 cut(s) 30, 353, 504, 536
HapII CCGG 3 cut(s) 124, 291, 508
HgaI GACGC 3 cut(s) 61, 143, 296
Hin1I GRCGYC 4 cut(s) 53, 135, 288, 474
Hin1II CATG 2 cut(s) 542, 883
HincII GTYRAC 2 cut(s) 948, 1120
HindII GTYRAC 2 cut(s) 948, 1120
HinfI GANTC 4 cut(s) 199, 701, 747, 1046
HpaI GTTAAC 1 cut(s) 948
HpaII CCGG 3 cut(s) 124, 291, 508
HphI GGTGA 2 cut(s) 77, 1028
Hpy166II GTNNAC 5 cut(s) 472, 948, 992, 1079, 1120
Hpy188I TCNGA 5 cut(s) 249, 299, 402, 562, 921
Hpy188III TCNNGA 7 cut(s) 316, 441, 676, 705, 822, 1154, 1171
Hpy8I GTNNAC 5 cut(s) 472, 948, 992, 1079, 1120
Hpy99I CGWCG 4 cut(s) 55, 137, 290, 1121
HpyAV CCTTC 3 cut(s) 196, 1060, 1102
HpyCH4III ACNGT 5 cut(s) 102, 373, 588, 1010, 1195
HpyCH4IV ACGT 4 cut(s) 423, 461, 474, 1116
HpyCH4V TGCA 4 cut(s) 110, 638, 758, 850
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 388, 533, 791
HpyF3I CTNAG 3 cut(s) 17, 729, 1152
HpySE526I ACGT 4 cut(s) 423, 461, 474, 1116
Hsp92I GRCGYC 4 cut(s) 53, 135, 288, 474
Hsp92II CATG 2 cut(s) 542, 883
Ksp22I TGATCA 1 cut(s) 672
KspAI GTTAAC 1 cut(s) 948
Kzo9I GATC 3 cut(s) 249, 672, 724
LmnI GCTCC 6 cut(s) 529, 562, 707, 713, 827, 1156
Lsp1109I GCAGC 4 cut(s) 366, 752, 786, 842
LweI GCATC 1 cut(s) 119
MaeII ACGT 4 cut(s) 423, 461, 474, 1116
MaeIII GTNAC 4 cut(s) 102, 367, 457, 611
MalI GATC 3 cut(s) 251, 674, 726
MbiI CCGCTC 3 cut(s) 62, 321, 565
MboI GATC 3 cut(s) 249, 672, 724
MboII GAAGA 6 cut(s) 154, 183, 427, 691, 778, 866
MfeI CAATTG 1 cut(s) 870
MhlI GDGCHC 3 cut(s) 355, 489, 718
MroXI GAANNNNTTC 2 cut(s) 905, 1091
MseI TTAA 4 cut(s) 684, 812, 947, 1161
MspI CCGG 3 cut(s) 124, 291, 508
MspR9I CCNGG 2 cut(s) 196, 509
MunI CAATTG 1 cut(s) 870
Mva1269I GAATGC 1 cut(s) 1184
MvaI CCWGG 1 cut(s) 196
MvnI CGCG 1 cut(s) 527
MwoI GCNNNNNNNGC 4 cut(s) 36, 388, 533, 791
NciI CCSGG 1 cut(s) 509
NdeII GATC 3 cut(s) 249, 672, 724
NlaIII CATG 2 cut(s) 542, 883
NlaIV GGNNCC 5 cut(s) 305, 352, 353, 715, 861
NmeAIII GCCGAG 1 cut(s) 6
NmuCI GTSAC 3 cut(s) 102, 367, 457
PctI GAATGC 1 cut(s) 1184
PdmI GAANNNNTTC 2 cut(s) 905, 1091
PfeI GAWTC 4 cut(s) 199, 701, 747, 1046
PflFI GACNNNGTC 1 cut(s) 55
PflMI CCANNNNNTGG 1 cut(s) 544
PfoI TCCNGGA 1 cut(s) 194
PkrI GCNGC 6 cut(s) 323, 381, 578, 742, 776, 832
Ppu21I YACGTR 1 cut(s) 424
Psp6I CCWGG 1 cut(s) 194
PspFI CCCAGC 1 cut(s) 382
PspGI CCWGG 1 cut(s) 194
PspN4I GGNNCC 5 cut(s) 305, 352, 353, 715, 861
PspOMI GGGCCC 1 cut(s) 351
PspPI GGNCC 4 cut(s) 351, 352, 502, 535
PsyI GACNNNGTC 1 cut(s) 55
RsaI GTAC 4 cut(s) 422, 434, 1007, 1012
RsaNI GTAC 4 cut(s) 421, 433, 1006, 1011
SalI GTCGAC 1 cut(s) 1118
SaqAI TTAA 4 cut(s) 684, 812, 947, 1161
SatI GCNGC 6 cut(s) 322, 380, 577, 741, 775, 831
Sau3AI GATC 3 cut(s) 249, 672, 724
Sau96I GGNCC 4 cut(s) 351, 352, 502, 535
ScrFI CCNGG 2 cut(s) 196, 509
SduI GDGCHC 3 cut(s) 355, 489, 718
SfaNI GCATC 1 cut(s) 119
SfcI CTRYAG 1 cut(s) 1191
StyD4I CCNGG 2 cut(s) 194, 507
StyI CCWWGG 1 cut(s) 951
TaaI ACNGT 5 cut(s) 102, 373, 588, 1010, 1195
TaiI ACGT 4 cut(s) 426, 464, 477, 1119
TaqI TCGA 3 cut(s) 275, 1044, 1119
TatI WGTACW 1 cut(s) 1005
TauI GCSGC 2 cut(s) 324, 579
TfiI GAWTC 4 cut(s) 199, 701, 747, 1046
Tru1I TTAA 4 cut(s) 684, 812, 947, 1161
Tru9I TTAA 4 cut(s) 684, 812, 947, 1161
TscAI CASTG 2 cut(s) 21, 1200
TseFI GTSAC 3 cut(s) 102, 367, 457
TseI GCWGC 4 cut(s) 379, 740, 774, 830
Tsp45I GTSAC 3 cut(s) 102, 367, 457
TspDTI ATGAA 2 cut(s) 779, 1042
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 2 cut(s) 21, 1200
Tth111I GACNNNGTC 1 cut(s) 55
Van91I CCANNNNNTGG 1 cut(s) 544
XapI RAATTY 4 cut(s) 21, 80, 215, 648
XcmI CCANNNNNNNNNTGG 1 cut(s) 886
XmiI GTMKAC 2 cut(s) 1078, 1119
XmnI GAANNNNTTC 2 cut(s) 905, 1091
ZraI GACGTC 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.