Rw2G011290

Transmembrane protein 53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014260
Physical Location & Seq
Forward (+)
11407120 .. 11409240
2121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G011290.1

Sequence Viewer

Length: 1197 bp
ATGGAAACAACCTCACTGAGAATTCTCGGCCTCAACCGCCACCTCAAAACGACGCCGTCCGCTCTTTTTCTCTCCCCCAAATTTCACCGACCTCAAAAACCCTCACTTGCATTTCCCAATTTCCGGCGAAACGACGCCGTGAAGAGAACCCCTTTTGCCCTTTCTCTCTCACATTCTCACTCCTCCTTCTCTTCCTGGAATCCAAACCCTTTCAAATTCAACCCCTCAATTTCCCAATCTCCATTTCCGATCACTAATTTCTACTACTCTCTGTCGAACCAAAACGACGCCGGTTTCAGATGGAACCGAGCGGTCCACAGCGGCATTGACGGAAATGTGGGATCTTTGGGGCCCCAAAAGCGGGAAGTGACGGTGGTGCTGCTGGGCTGGCTTGGGGGTCAGACCAAGCACCTGAAGAGGTACGTGGAGTGGTACAATTCCCGAGGCTTTCACGCTGTGACGTTTGTTGTGGACGTCAAGGAGGTGCTCTGGTTTGATTTGGGCCACCGGGTTGAGAAGCGGGTCGCGGAGCTGGCCCATGAGCTGGCTTCGTGGGTTGAGGGCGGCGGAGAACGGTGTATGGTGTTTCACACTTTTAGTAACACGGGTTGGTTTGTATATGGTGCAATACTTGAAATTTGGCAGGATAGACCAGACTTGATCAAGAAGATTAAAGGGTGTATTGTTGATTCAGGAGCTGGAGCCCCTTTTGACCCTAAGGTGTGGGCAGCAGGATTCTCTACTGCGATTATGAAGAAACGCAGCTCTTTAGCAAAACCTGCGGTTGAGACTAAAGAATTAAATGCCTCTGGAGGAGCAGCGAGCTTGTCAAAGGTGCAAGAAGAGGAACCTCCAGCAATTGAAACCATGGTTCTGTTGGTATTAGAGAAGCTGTTCTCACTCATTCTGAAGTTGCCTGATGTGGATAAGAGGTTAACCAAGGTAGTTTCTGTCCTCTCGACAAACCCTCCTCATTGTCCACAGCTTTACCTGTACAGTACGGGTGATAAAGTCGTCCCACACCAGTCCATCGAATCCTTCATCGCAGAGCAAAGGCAGATAGGTAGACCCGTGAAGTCCTTCAATTTCGGCACATCTCCTCACGTCGACCATTACAGGACTTTCCCCAACATATACTCTCAGGAGCTTAACAATTTCTTGAAAGAATGCTTGGCTACAACAGTTAAGCAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

398

Amino Acids

44.74

Weight (kDa)

9.51

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 124 - 387 4.7e-63 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18245
fragaria_vesca FvH4_1g13010
malus_domestica MD02G1144600.v1.1 MD15G1258300.v1.1
prunus_persica Prupe.7G157000_v2.0.a1 Prupe.7G157000_v2.0.a1
pyrus_communis pycom02g11450 pycom15g22610
rosa_chinensis RchiOBHm_Chr2g0101161
rosa_laevigata RLG00000017051
rosa_roxburghii Rroxscaffold_2G00141530
rosa_rugosa Rorug02G0100600
rosa_samantha Rh2AG144000 Rh2BG149000 Rh2CG150300 Rh2DG149500
rosa_wichuraiana Rw2G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 477
Acc36I ACCTGC 1 cut(s) 787
AccB7I CCANNNNNTGG 1 cut(s) 544
AccBSI CCGCTC 2 cut(s) 62, 311
AccI GTMKAC 2 cut(s) 1066, 1107
AccII CGCG 1 cut(s) 527
AclWI GGATC 1 cut(s) 349
AcsI RAATTY 4 cut(s) 21, 80, 215, 636
AcuI CTGAAG 2 cut(s) 434, 929
AcyI GRCGYC 4 cut(s) 53, 135, 288, 474
AdeI CACNNNGTG 1 cut(s) 457
AfaI GTAC 4 cut(s) 422, 434, 995, 1000
AfiI CCNNNNNNNGG 4 cut(s) 123, 360, 361, 544
AgsI TTSAA 6 cut(s) 214, 220, 635, 863, 1084, 1162
AjiI CACGTC 1 cut(s) 1105
AjnI CCWGG 1 cut(s) 194
AjuI GAANNNNNNNTTGG 4 cut(s) 228, 260, 1154, 1186
AluBI AGCT 8 cut(s) 532, 544, 698, 765, 825, 892, 985, 1147
AluI AGCT 8 cut(s) 532, 544, 698, 765, 825, 892, 985, 1147
Alw21I GWGCWC 1 cut(s) 489
Alw26I GTCTC 1 cut(s) 782
AlwI GGATC 1 cut(s) 349
AlwNI CAGNNNCTG 1 cut(s) 698
Ama87I CYCGRG 1 cut(s) 441
AoxI GGCC 4 cut(s) 28, 350, 502, 534
ApaI GGGCCC 1 cut(s) 354
ApeKI GCWGC 4 cut(s) 379, 728, 762, 818
ApoI RAATTY 4 cut(s) 21, 80, 215, 636
Asp700I GAANNNNTTC 2 cut(s) 893, 1079
AspS9I GGNCC 5 cut(s) 313, 350, 351, 502, 535
AsuC2I CCSGG 1 cut(s) 509
AsuHPI GGTGA 2 cut(s) 77, 1016
AvaI CYCGRG 1 cut(s) 441
AvaII GGWCC 1 cut(s) 313
AxyI CCTNAGG 1 cut(s) 717
BaeGI GKGCMC 1 cut(s) 354
BanII GRGCYC 2 cut(s) 354, 706
Bbv12I GWGCWC 1 cut(s) 489
BbvI GCAGC 4 cut(s) 366, 740, 774, 830
BccI CCATC 2 cut(s) 294, 1037
BceAI ACGGC 2 cut(s) 40, 122
BciT130I CCWGG 1 cut(s) 196
BclI TGATCA 1 cut(s) 660
BcnI CCSGG 1 cut(s) 509
BcoDI GTCTC 1 cut(s) 782
BfuAI ACCTGC 1 cut(s) 787
BisI GCNGC 6 cut(s) 322, 380, 565, 729, 763, 819
BlsI GCNGC 6 cut(s) 323, 381, 566, 730, 764, 820
Bme1390I CCNGG 2 cut(s) 196, 509
Bme18I GGWCC 1 cut(s) 313
BmeT110I CYCGRG 1 cut(s) 441
BmgBI CACGTC 1 cut(s) 1105
BmgT120I GGNCC 5 cut(s) 313, 350, 351, 502, 535
BmiI GGNNCC 6 cut(s) 305, 351, 352, 353, 703, 849
BmrFI CCNGG 2 cut(s) 196, 509
BpmI CTGGAG 3 cut(s) 720, 831, 837
BpuMI CCSGG 1 cut(s) 509
BsaAI YACGTR 1 cut(s) 424
BsaHI GRCGYC 4 cut(s) 53, 135, 288, 474
BsaJI CCNNGG 3 cut(s) 442, 867, 939
BsaXI ACNNNNNCTCC 2 cut(s) 952, 982
Bsc4I CCNNNNNNNGG 4 cut(s) 123, 360, 361, 544
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 1024
Bse21I CCTNAGG 1 cut(s) 717
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 3 cut(s) 442, 867, 939
BseLI CCNNNNNNNGG 4 cut(s) 123, 360, 361, 544
BseMII CTCAG 2 cut(s) 8, 1154
BseNI ACTGG 1 cut(s) 1024
BseRI GAGGAG 4 cut(s) 172, 828, 960, 1089
BseSI GKGCMC 1 cut(s) 354
BseXI GCAGC 4 cut(s) 366, 740, 774, 830
BseYI CCCAGC 1 cut(s) 382
Bsh1236I CGCG 1 cut(s) 527
BshFI GGCC 4 cut(s) 30, 352, 504, 536
BsiHKAI GWGCWC 1 cut(s) 489
BsiHKCI CYCGRG 1 cut(s) 441
BsiSI CCGG 3 cut(s) 124, 291, 508
BslFI GGGAC 1 cut(s) 1001
BslI CCNNNNNNNGG 4 cut(s) 123, 360, 361, 544
BsmAI GTCTC 1 cut(s) 782
BsmFI GGGAC 1 cut(s) 1001
BsmI GAATGC 1 cut(s) 1172
BsnI GGCC 4 cut(s) 30, 352, 504, 536
BsoBI CYCGRG 1 cut(s) 441
Bsp120I GGGCCC 1 cut(s) 350
Bsp1286I GDGCHC 3 cut(s) 354, 489, 706
Bsp1407I TGTACA 1 cut(s) 993
Bsp143I GATC 3 cut(s) 249, 341, 660
Bsp19I CCATGG 1 cut(s) 867
BspANI GGCC 4 cut(s) 30, 352, 504, 536
BspCNI CTCAG 2 cut(s) 9, 1153
BspFNI CGCG 1 cut(s) 527
BspLI GGNNCC 6 cut(s) 305, 351, 352, 353, 703, 849
BspMI ACCTGC 1 cut(s) 787
BspPI GGATC 1 cut(s) 349
BsrBI CCGCTC 2 cut(s) 62, 311
BsrFI RCCGGY 1 cut(s) 290
BsrGI TGTACA 1 cut(s) 993
BsrI ACTGG 1 cut(s) 1024
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 3 cut(s) 442, 867, 939
BssMI GATC 3 cut(s) 249, 341, 660
BssNI GRCGYC 4 cut(s) 53, 135, 288, 474
BssT1I CCWWGG 2 cut(s) 867, 939
Bst2UI CCWGG 1 cut(s) 196
Bst4CI ACNGT 4 cut(s) 373, 576, 998, 1183
Bst6I CTCTTC 4 cut(s) 137, 196, 410, 837
BstACI GRCGYC 4 cut(s) 53, 135, 288, 474
BstAPI GCANNNNNTGC 1 cut(s) 779
BstAUI TGTACA 1 cut(s) 993
BstBAI YACGTR 1 cut(s) 424
BstC8I GCNNGC 4 cut(s) 389, 534, 546, 823
BstDEI CTNAG 3 cut(s) 17, 717, 1140
BstDSI CCRYGG 1 cut(s) 867
BstFNI CGCG 1 cut(s) 527
BstKTI GATC 3 cut(s) 252, 344, 663
BstMAI GTCTC 1 cut(s) 782
BstMBI GATC 3 cut(s) 249, 341, 660
BstMWI GCNNNNNNNGC 5 cut(s) 36, 358, 388, 533, 779
BstNI CCWGG 1 cut(s) 196
BstSCI CCNGG 2 cut(s) 194, 507
BstSLI GKGCMC 1 cut(s) 354
BstUI CGCG 1 cut(s) 527
BstV1I GCAGC 4 cut(s) 366, 740, 774, 830
BstX2I RGATCY 1 cut(s) 341
BstYI RGATCY 1 cut(s) 341
Bsu36I CCTNAGG 1 cut(s) 717
BsuRI GGCC 4 cut(s) 30, 352, 504, 536
BtgI CCRYGG 1 cut(s) 867
BtgZI GCGATG 1 cut(s) 1027
BtrI CACGTC 1 cut(s) 1105
BtsIMutI CAGTG 1 cut(s) 14
BveI ACCTGC 1 cut(s) 787
Cac8I GCNNGC 4 cut(s) 389, 534, 546, 823
CaiI CAGNNNCTG 1 cut(s) 698
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 5 cut(s) 313, 350, 351, 502, 535
CseI GACGC 3 cut(s) 61, 143, 296
Csp6I GTAC 4 cut(s) 421, 433, 994, 999
CviAII CATG 2 cut(s) 539, 868
CviQI GTAC 4 cut(s) 421, 433, 994, 999
DdeI CTNAG 3 cut(s) 17, 717, 1140
DpnI GATC 3 cut(s) 251, 343, 662
DpnII GATC 3 cut(s) 249, 341, 660
DraIII CACNNNGTG 1 cut(s) 457
Eam1104I CTCTTC 4 cut(s) 137, 196, 410, 837
EarI CTCTTC 4 cut(s) 137, 196, 410, 837
EciI GGCGGA 1 cut(s) 582
Eco130I CCWWGG 2 cut(s) 867, 939
Eco24I GRGCYC 2 cut(s) 354, 706
Eco47I GGWCC 1 cut(s) 313
Eco57I CTGAAG 2 cut(s) 434, 929
Eco81I CCTNAGG 1 cut(s) 717
Eco88I CYCGRG 1 cut(s) 441
EcoO109I RGGNCCY 2 cut(s) 350, 351
EcoRI GAATTC 1 cut(s) 21
EcoRII CCWGG 1 cut(s) 194
EcoT14I CCWWGG 2 cut(s) 867, 939
EcoT38I GRGCYC 2 cut(s) 354, 706
ErhI CCWWGG 2 cut(s) 867, 939
FaeI CATG 2 cut(s) 542, 871
FaiI YATR 8 cut(s) 540, 581, 619, 621, 752, 869, 1133, 1135
FaqI GGGAC 1 cut(s) 1001
FatI CATG 2 cut(s) 538, 867
FauI CCCGC 2 cut(s) 354, 513
FbaI TGATCA 1 cut(s) 660
FblI GTMKAC 2 cut(s) 1066, 1107
Fnu4HI GCNGC 6 cut(s) 322, 380, 565, 729, 763, 819
FriOI GRGCYC 2 cut(s) 354, 706
Fsp4HI GCNGC 6 cut(s) 322, 380, 565, 729, 763, 819
GluI GCNGC 6 cut(s) 322, 380, 565, 729, 763, 819
GsaI CCCAGC 1 cut(s) 386
GsuI CTGGAG 3 cut(s) 720, 831, 837
HaeIII GGCC 4 cut(s) 30, 352, 504, 536
HapII CCGG 3 cut(s) 124, 291, 508
HgaI GACGC 3 cut(s) 61, 143, 296
Hin1I GRCGYC 4 cut(s) 53, 135, 288, 474
Hin1II CATG 2 cut(s) 542, 871
HincII GTYRAC 2 cut(s) 936, 1108
HindII GTYRAC 2 cut(s) 936, 1108
HinfI GANTC 4 cut(s) 199, 689, 735, 1034
HpaI GTTAAC 1 cut(s) 936
HpaII CCGG 3 cut(s) 124, 291, 508
HphI GGTGA 2 cut(s) 77, 1016
Hpy166II GTNNAC 6 cut(s) 316, 472, 936, 980, 1067, 1108
Hpy188I TCNGA 4 cut(s) 249, 299, 402, 909
Hpy188III TCNNGA 7 cut(s) 441, 664, 693, 810, 958, 1142, 1159
Hpy8I GTNNAC 6 cut(s) 316, 472, 936, 980, 1067, 1108
Hpy99I CGWCG 4 cut(s) 55, 137, 290, 1109
HpyAV CCTTC 3 cut(s) 196, 1048, 1090
HpyCH4III ACNGT 4 cut(s) 373, 576, 998, 1183
HpyCH4IV ACGT 4 cut(s) 423, 461, 474, 1104
HpyCH4V TGCA 3 cut(s) 110, 626, 838
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 358, 388, 533, 779
HpyF3I CTNAG 3 cut(s) 17, 717, 1140
HpySE526I ACGT 4 cut(s) 423, 461, 474, 1104
Hsp92I GRCGYC 4 cut(s) 53, 135, 288, 474
Hsp92II CATG 2 cut(s) 542, 871
Ksp22I TGATCA 1 cut(s) 660
KspAI GTTAAC 1 cut(s) 936
Kzo9I GATC 3 cut(s) 249, 341, 660
LmnI GCTCC 5 cut(s) 529, 695, 701, 815, 1144
Lsp1109I GCAGC 4 cut(s) 366, 740, 774, 830
MaeII ACGT 4 cut(s) 423, 461, 474, 1104
MaeIII GTNAC 3 cut(s) 367, 457, 599
MalI GATC 3 cut(s) 251, 343, 662
MbiI CCGCTC 2 cut(s) 62, 311
MboI GATC 3 cut(s) 249, 341, 660
MboII GAAGA 6 cut(s) 154, 183, 427, 679, 766, 854
MfeI CAATTG 1 cut(s) 858
MflI RGATCY 1 cut(s) 341
MhlI GDGCHC 3 cut(s) 354, 489, 706
MroXI GAANNNNTTC 2 cut(s) 893, 1079
MseI TTAA 5 cut(s) 672, 800, 935, 1149, 1185
MspA1I CMGCKG 1 cut(s) 321
MspI CCGG 3 cut(s) 124, 291, 508
MspR9I CCNGG 2 cut(s) 196, 509
MunI CAATTG 1 cut(s) 858
Mva1269I GAATGC 1 cut(s) 1172
MvaI CCWGG 1 cut(s) 196
MvnI CGCG 1 cut(s) 527
MwoI GCNNNNNNNGC 5 cut(s) 36, 358, 388, 533, 779
NciI CCSGG 1 cut(s) 509
NcoI CCATGG 1 cut(s) 867
NdeII GATC 3 cut(s) 249, 341, 660
NlaIII CATG 2 cut(s) 542, 871
NlaIV GGNNCC 6 cut(s) 305, 351, 352, 353, 703, 849
NmeAIII GCCGAG 1 cut(s) 6
NmuCI GTSAC 2 cut(s) 367, 457
PctI GAATGC 1 cut(s) 1172
PdmI GAANNNNTTC 2 cut(s) 893, 1079
PfeI GAWTC 4 cut(s) 199, 689, 735, 1034
PflFI GACNNNGTC 1 cut(s) 55
PflMI CCANNNNNTGG 1 cut(s) 544
PfoI TCCNGGA 1 cut(s) 194
PkrI GCNGC 6 cut(s) 323, 381, 566, 730, 764, 820
Ppu21I YACGTR 1 cut(s) 424
Psp6I CCWGG 1 cut(s) 194
PspFI CCCAGC 1 cut(s) 382
PspGI CCWGG 1 cut(s) 194
PspN4I GGNNCC 6 cut(s) 305, 351, 352, 353, 703, 849
PspOMI GGGCCC 1 cut(s) 350
PspPI GGNCC 5 cut(s) 313, 350, 351, 502, 535
PstNI CAGNNNCTG 1 cut(s) 698
PsuI RGATCY 1 cut(s) 341
PsyI GACNNNGTC 1 cut(s) 55
RsaI GTAC 4 cut(s) 422, 434, 995, 1000
RsaNI GTAC 4 cut(s) 421, 433, 994, 999
SalI GTCGAC 1 cut(s) 1106
SaqAI TTAA 5 cut(s) 672, 800, 935, 1149, 1185
SatI GCNGC 6 cut(s) 322, 380, 565, 729, 763, 819
Sau3AI GATC 3 cut(s) 249, 341, 660
Sau96I GGNCC 5 cut(s) 313, 350, 351, 502, 535
ScrFI CCNGG 2 cut(s) 196, 509
SduI GDGCHC 3 cut(s) 354, 489, 706
SinI GGWCC 1 cut(s) 313
StyD4I CCNGG 2 cut(s) 194, 507
StyI CCWWGG 2 cut(s) 867, 939
TaaI ACNGT 4 cut(s) 373, 576, 998, 1183
TaiI ACGT 4 cut(s) 426, 464, 477, 1107
TaqI TCGA 4 cut(s) 275, 959, 1032, 1107
TatI WGTACW 1 cut(s) 993
TauI GCSGC 2 cut(s) 324, 567
TfiI GAWTC 4 cut(s) 199, 689, 735, 1034
Tru1I TTAA 5 cut(s) 672, 800, 935, 1149, 1185
Tru9I TTAA 5 cut(s) 672, 800, 935, 1149, 1185
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 2 cut(s) 367, 457
TseI GCWGC 4 cut(s) 379, 728, 762, 818
Tsp45I GTSAC 2 cut(s) 367, 457
TspDTI ATGAA 2 cut(s) 767, 1030
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 1 cut(s) 21
Tth111I GACNNNGTC 1 cut(s) 55
Van91I CCANNNNNTGG 1 cut(s) 544
VpaK11BI GGWCC 1 cut(s) 313
XapI RAATTY 4 cut(s) 21, 80, 215, 636
XcmI CCANNNNNNNNNTGG 1 cut(s) 874
XmiI GTMKAC 2 cut(s) 1066, 1107
XmnI GAANNNNTTC 2 cut(s) 893, 1079
ZraI GACGTC 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.