MD02G1144600.v1.1

Transmembrane protein 53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014260
Physical Location & Seq
Forward (+)
11975930 .. 11978146
2217 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1144600.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGGAAACTCCGGCGAGAATTCTCAGCCTCAACCTCCAGCTCTTCGCCAGAACGACACCGTCCGCCCTCGCTTTCTCTCCCAAACTCCACAGACCCGCGAAGCCCTTGCCTACATTTCACACTCTCGCTCGAAACAGCGCCGTCAAAAGAACCCCCTTTTCTCTTTCGCTCGCGCAGCCGCACTCCTCGTTCTCCTTCAATCCGAATCCCTTCAAATCCCTCCCCCTTTCTCTCTCCTCCCTGTCGCCGTTCAGCAGCTTCAACCACTCTCTCTCACCCTCAAACGACGGCGTTTCCACCTGGAACCGGGCGTTCCACGGCGGGATTAACGGAAATGTGGGTTCTTTCGGGACCCACAACCGTGAAGTGACGGTGGTGTTGCTAGGCTGGCTCGGGGCCAAGACGAAGCACCTAAAGAGGTACGTGGAGTGGTACAACTCCAGAGGCATTCACGCCGTGACCTTCGTCGTTGATCCGAGGGAGGTTCTCTGGTTTGATTTGGGCCGCCGGGTCGAAAATCGGGTTTCGGACTTGGCCCGGGATCTCGCTTCGTGGCTGGAGGAGGGCGGAGAAAGGTGCTTGCTTTTCCACACCTTCAGCAACACGGGTTGGTATGGCGCCATTCTTGAAATTCTGCAGGGGAGGAAAGACCTGATGGAGAAGATTAAAGGGTGTGTTGTTGATTCAGGAGCAGCTGAACCGTTTAATCCTAAGGTGTGGGCAGCAGGGTTTTCTGCTGCGATTTTGAAGAAGCACAGCTCTTTAACAAGCTCTGCGGTTGAGGCTAGAGATTTAAATGAGTCGGAAGATGCAGCAAGCTTATCAAAGCTGCAAGAAGAAAAGCCTCCGCTAGTGGAAGCCGTGGTTCTACTGATGTTAGAGAAGCTGTTTTCGGTTCTTCTAAAGTTTCCTGATGTGGATAAGAGGTTAACGAAGGTAGTTTCCATTCTTTCGGAAAACACTCCTTATTGCCCGCAACTTTACCTATATAGCACTGCCGATAAAGTCGTTCCACACCAGTCAGTCGAGTTGTTCATGGCGGAGCAACAGCGGAAGGGTAGAATTGTAAGGTCCTTCAATTTCGGCACAACACCTCATGTGGATCACTTCAGGAATTTCCCCGACATATACTGTTCAGAGCTTGACAGTTTCTTGAAAGATTGTTTAACTACAGTTGTGACGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

396

Amino Acids

44.11

Weight (kDa)

8.98

Isoelectric Point (pI)

36.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 124 - 385 3.5e-57 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18245
fragaria_vesca FvH4_1g13010
malus_domestica MD02G1144600.v1.1 MD15G1258300.v1.1
prunus_persica Prupe.7G157000_v2.0.a1 Prupe.7G157000_v2.0.a1
pyrus_communis pycom02g11450 pycom15g22610
rosa_chinensis RchiOBHm_Chr2g0101161
rosa_laevigata RLG00000017051
rosa_roxburghii Rroxscaffold_2G00141530
rosa_rugosa Rorug02G0100600
rosa_samantha Rh2AG144000 Rh2BG149000 Rh2CG150300 Rh2DG149500
rosa_wichuraiana Rw2G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 617
AccII CGCG 2 cut(s) 98, 173
AclWI GGATC 3 cut(s) 467, 549, 1110
AcsI RAATTY 3 cut(s) 18, 630, 1114
AcuI CTGAAG 2 cut(s) 580, 1093
AcyI GRCGYC 1 cut(s) 618
AdeI CACNNNGTG 1 cut(s) 457
AfaI GTAC 2 cut(s) 422, 434
AfiI CCNNNNNNNGG 1 cut(s) 306
AgsI TTSAA 7 cut(s) 199, 214, 262, 629, 748, 1078, 1156
AjiI CACGTC 1 cut(s) 1185
AjnI CCWGG 1 cut(s) 299
AleI CACNNNNGTG 1 cut(s) 360
AluBI AGCT 9 cut(s) 40, 258, 695, 759, 771, 819, 829, 886, 1141
AluI AGCT 9 cut(s) 40, 258, 695, 759, 771, 819, 829, 886, 1141
AlwI GGATC 3 cut(s) 467, 549, 1110
Ama87I CYCGRG 2 cut(s) 392, 537
AoxI GGCC 3 cut(s) 396, 502, 534
ApeKI GCWGC 7 cut(s) 175, 255, 692, 722, 737, 812, 829
ApoI RAATTY 3 cut(s) 18, 630, 1114
Asp700I GAANNNNTTC 1 cut(s) 209
AspLEI GCGC 3 cut(s) 140, 175, 620
AspS9I GGNCC 5 cut(s) 351, 396, 502, 535, 1071
AsuC2I CCSGG 4 cut(s) 308, 509, 538, 539
AsuHPI GGTGA 1 cut(s) 267
AvaI CYCGRG 2 cut(s) 392, 537
AvaII GGWCC 2 cut(s) 351, 1071
AxyI CCTNAGG 1 cut(s) 711
BanI GGYRCC 1 cut(s) 617
BbvI GCAGC 7 cut(s) 187, 267, 704, 724, 734, 816, 824
BccI CCATC 1 cut(s) 649
BceAI ACGGC 6 cut(s) 125, 232, 304, 334, 440, 845
BcgI CGANNNNNNTGC 2 cut(s) 88, 122
BciT130I CCWGG 1 cut(s) 301
BcnI CCSGG 4 cut(s) 308, 509, 538, 539
BfaI CTAG 3 cut(s) 383, 786, 851
BfmI CTRYAG 2 cut(s) 635, 1170
BfoI RGCGCY 2 cut(s) 141, 621
BisI GCNGC 9 cut(s) 176, 179, 256, 505, 693, 723, 738, 813, 830
BlsI GCNGC 9 cut(s) 177, 180, 257, 506, 694, 724, 739, 814, 831
Bme1390I CCNGG 5 cut(s) 301, 308, 509, 538, 539
Bme18I GGWCC 2 cut(s) 351, 1071
BmeT110I CYCGRG 2 cut(s) 392, 537
BmgBI CACGTC 1 cut(s) 1185
BmgT120I GGNCC 5 cut(s) 351, 396, 502, 535, 1071
BmiI GGNNCC 5 cut(s) 305, 352, 353, 397, 619
BmrFI CCNGG 5 cut(s) 301, 308, 509, 538, 539
BmsI GCATC 1 cut(s) 799
BoxI GACNNNNGTC 1 cut(s) 464
BpmI CTGGAG 3 cut(s) 20, 424, 578
BpuMI CCSGG 4 cut(s) 308, 509, 538, 539
BsaAI YACGTR 1 cut(s) 424
BsaHI GRCGYC 1 cut(s) 618
BsaJI CCNNGG 4 cut(s) 316, 476, 537, 861
Bsc4I CCNNNNNNNGG 1 cut(s) 306
Bse1I ACTGG 1 cut(s) 1018
Bse21I CCTNAGG 1 cut(s) 711
BseBI CCWGG 1 cut(s) 301
BseDI CCNNGG 4 cut(s) 316, 476, 537, 861
BseLI CCNNNNNNNGG 1 cut(s) 306
BseMII CTCAG 1 cut(s) 37
BseNI ACTGG 1 cut(s) 1018
BseRI GAGGAG 3 cut(s) 175, 226, 575
BseXI GCAGC 7 cut(s) 187, 267, 704, 724, 734, 816, 824
Bsh1236I CGCG 2 cut(s) 98, 173
BshFI GGCC 3 cut(s) 398, 504, 536
BshNI GGYRCC 1 cut(s) 617
BsiHKCI CYCGRG 2 cut(s) 392, 537
BsiSI CCGG 4 cut(s) 11, 307, 508, 538
BslFI GGGAC 1 cut(s) 364
BslI CCNNNNNNNGG 1 cut(s) 306
BsmFI GGGAC 1 cut(s) 364
BsmI GAATGC 1 cut(s) 447
BsnI GGCC 3 cut(s) 398, 504, 536
BsoBI CYCGRG 2 cut(s) 392, 537
Bsp143I GATC 3 cut(s) 472, 541, 1102
BspANI GGCC 3 cut(s) 398, 504, 536
BspCNI CTCAG 1 cut(s) 36
BspFNI CGCG 2 cut(s) 98, 173
BspLI GGNNCC 5 cut(s) 305, 352, 353, 397, 619
BspMAI CTGCAG 1 cut(s) 639
BspPI GGATC 3 cut(s) 467, 549, 1110
BspQI GCTCTTC 1 cut(s) 47
BspT107I GGYRCC 1 cut(s) 617
BsrI ACTGG 1 cut(s) 1018
BssECI CCNNGG 4 cut(s) 316, 476, 537, 861
BssMI GATC 3 cut(s) 472, 541, 1102
BssNI GRCGYC 1 cut(s) 618
Bst2UI CCWGG 1 cut(s) 301
Bst4CI ACNGT 7 cut(s) 60, 362, 373, 702, 1133, 1148, 1174
Bst6I CTCTTC 1 cut(s) 47
BstACI GRCGYC 1 cut(s) 618
BstBAI YACGTR 1 cut(s) 424
BstC8I GCNNGC 5 cut(s) 171, 389, 581, 817, 974
BstDEI CTNAG 2 cut(s) 23, 711
BstDSI CCRYGG 2 cut(s) 316, 861
BstFNI CGCG 2 cut(s) 98, 173
BstH2I RGCGCY 2 cut(s) 141, 621
BstHHI GCGC 3 cut(s) 140, 175, 620
BstKTI GATC 3 cut(s) 475, 544, 1105
BstMBI GATC 3 cut(s) 472, 541, 1102
BstMWI GCNNNNNNNGC 3 cut(s) 175, 388, 782
BstNI CCWGG 1 cut(s) 301
BstPAI GACNNNNGTC 1 cut(s) 464
BstSCI CCNGG 5 cut(s) 299, 306, 507, 536, 537
BstSFI CTRYAG 2 cut(s) 635, 1170
BstUI CGCG 2 cut(s) 98, 173
BstV1I GCAGC 7 cut(s) 187, 267, 704, 724, 734, 816, 824
BstX2I RGATCY 1 cut(s) 541
BstYI RGATCY 1 cut(s) 541
Bsu36I CCTNAGG 1 cut(s) 711
BsuRI GGCC 3 cut(s) 398, 504, 536
BtgI CCRYGG 2 cut(s) 316, 861
BtrI CACGTC 1 cut(s) 1185
BtsI GCAGTG 1 cut(s) 993
BtsIMutI CAGTG 1 cut(s) 993
Cac8I GCNNGC 5 cut(s) 171, 389, 581, 817, 974
CfoI GCGC 3 cut(s) 140, 175, 620
Cfr13I GGNCC 5 cut(s) 351, 396, 502, 535, 1071
Cfr9I CCCGGG 1 cut(s) 537
Csp6I GTAC 2 cut(s) 421, 433
CviAII CATG 2 cut(s) 1036, 1097
CviQI GTAC 2 cut(s) 421, 433
DdeI CTNAG 2 cut(s) 23, 711
DinI GGCGCC 1 cut(s) 619
DpnI GATC 3 cut(s) 474, 543, 1104
DpnII GATC 3 cut(s) 472, 541, 1102
DraI TTTAAA 1 cut(s) 795
DraIII CACNNNGTG 1 cut(s) 457
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
EciI GGCGGA 3 cut(s) 52, 582, 1055
Eco47I GGWCC 2 cut(s) 351, 1071
Eco57I CTGAAG 2 cut(s) 580, 1093
Eco81I CCTNAGG 1 cut(s) 711
Eco88I CYCGRG 2 cut(s) 392, 537
EcoO109I RGGNCCY 2 cut(s) 351, 1071
EcoRI GAATTC 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 299
EgeI GGCGCC 1 cut(s) 619
EheI GGCGCC 1 cut(s) 619
FaeI CATG 2 cut(s) 1039, 1100
FaiI YATR 7 cut(s) 615, 988, 990, 1037, 1098, 1127, 1129
FaqI GGGAC 1 cut(s) 364
FatI CATG 2 cut(s) 1035, 1096
FauI CCCGC 3 cut(s) 103, 314, 981
Fnu4HI GCNGC 9 cut(s) 176, 179, 256, 505, 693, 723, 738, 813, 830
Fsp4HI GCNGC 9 cut(s) 176, 179, 256, 505, 693, 723, 738, 813, 830
FspBI CTAG 3 cut(s) 383, 786, 851
GlaI GCGC 3 cut(s) 139, 174, 619
GluI GCNGC 9 cut(s) 176, 179, 256, 505, 693, 723, 738, 813, 830
GsuI CTGGAG 3 cut(s) 20, 424, 578
HaeII RGCGCY 2 cut(s) 141, 621
HaeIII GGCC 3 cut(s) 398, 504, 536
HapII CCGG 4 cut(s) 11, 307, 508, 538
HhaI GCGC 3 cut(s) 140, 175, 620
Hin1I GRCGYC 1 cut(s) 618
Hin1II CATG 2 cut(s) 1039, 1100
Hin6I GCGC 3 cut(s) 138, 173, 618
HinP1I GCGC 3 cut(s) 138, 173, 618
HincII GTYRAC 1 cut(s) 930
HindII GTYRAC 1 cut(s) 930
HindIII AAGCTT 1 cut(s) 817
HinfI GANTC 3 cut(s) 205, 683, 800
HpaI GTTAAC 1 cut(s) 930
HpaII CCGG 4 cut(s) 11, 307, 508, 538
HphI GGTGA 1 cut(s) 267
Hpy166II GTNNAC 1 cut(s) 930
Hpy188I TCNGA 6 cut(s) 204, 477, 529, 805, 955, 1138
Hpy188III TCNNGA 7 cut(s) 349, 441, 626, 687, 911, 1111, 1153
Hpy8I GTNNAC 1 cut(s) 930
Hpy99I CGWCG 3 cut(s) 290, 470, 1186
HpyAV CCTTC 7 cut(s) 205, 220, 472, 604, 928, 1048, 1084
HpyCH4III ACNGT 7 cut(s) 60, 362, 373, 702, 1133, 1148, 1174
HpyCH4IV ACGT 2 cut(s) 423, 1184
HpyCH4V TGCA 3 cut(s) 637, 812, 832
HpyF10VI GCNNNNNNNGC 3 cut(s) 175, 388, 782
HpyF3I CTNAG 2 cut(s) 23, 711
HpySE526I ACGT 2 cut(s) 423, 1184
Hsp92I GRCGYC 1 cut(s) 618
Hsp92II CATG 2 cut(s) 1039, 1100
HspAI GCGC 3 cut(s) 138, 173, 618
KasI GGCGCC 1 cut(s) 617
KflI GGGWCCC 1 cut(s) 351
KspAI GTTAAC 1 cut(s) 930
Kzo9I GATC 3 cut(s) 472, 541, 1102
LguI GCTCTTC 1 cut(s) 47
LmnI GCTCC 2 cut(s) 689, 1042
Lsp1109I GCAGC 7 cut(s) 187, 267, 704, 724, 734, 816, 824
LweI GCATC 1 cut(s) 799
MaeI CTAG 3 cut(s) 383, 786, 851
MaeII ACGT 2 cut(s) 423, 1184
MaeIII GTNAC 3 cut(s) 367, 457, 1177
MalI GATC 3 cut(s) 474, 543, 1104
MboI GATC 3 cut(s) 472, 541, 1102
MboII GAAGA 6 cut(s) 34, 673, 760, 818, 848, 890
MflI RGATCY 1 cut(s) 541
MluCI AATT 5 cut(s) 18, 630, 1062, 1078, 1114
Mly113I GGCGCC 1 cut(s) 618
MlyI GAGTC 1 cut(s) 809
MmeI TCCRAC 1 cut(s) 783
MroXI GAANNNNTTC 1 cut(s) 209
MseI TTAA 7 cut(s) 327, 666, 705, 764, 794, 929, 1166
MslI CAYNNNNRTG 1 cut(s) 360
MspA1I CMGCKG 2 cut(s) 695, 1051
MspI CCGG 4 cut(s) 11, 307, 508, 538
MspR9I CCNGG 5 cut(s) 301, 308, 509, 538, 539
Mva1269I GAATGC 1 cut(s) 447
MvaI CCWGG 1 cut(s) 301
MvnI CGCG 2 cut(s) 98, 173
MwoI GCNNNNNNNGC 3 cut(s) 175, 388, 782
NarI GGCGCC 1 cut(s) 618
NciI CCSGG 4 cut(s) 308, 509, 538, 539
NdeII GATC 3 cut(s) 472, 541, 1102
NlaIII CATG 2 cut(s) 1039, 1100
NlaIV GGNNCC 5 cut(s) 305, 352, 353, 397, 619
NmuCI GTSAC 3 cut(s) 367, 457, 1177
OliI CACNNNNGTG 1 cut(s) 360
PciSI GCTCTTC 1 cut(s) 47
PctI GAATGC 1 cut(s) 447
PdmI GAANNNNTTC 1 cut(s) 209
PfeI GAWTC 2 cut(s) 205, 683
PflFI GACNNNGTC 1 cut(s) 58
PkrI GCNGC 9 cut(s) 177, 180, 257, 506, 694, 724, 739, 814, 831
PleI GAGTC 1 cut(s) 808
PluTI GGCGCC 1 cut(s) 621
PpsI GAGTC 1 cut(s) 808
Ppu21I YACGTR 1 cut(s) 424
PpuMI RGGWCCY 2 cut(s) 351, 1071
PshAI GACNNNNGTC 1 cut(s) 464
Psp5II RGGWCCY 2 cut(s) 351, 1071
Psp6I CCWGG 1 cut(s) 299
PspGI CCWGG 1 cut(s) 299
PspN4I GGNNCC 5 cut(s) 305, 352, 353, 397, 619
PspPI GGNCC 5 cut(s) 351, 396, 502, 535, 1071
PspPPI RGGWCCY 2 cut(s) 351, 1071
PstI CTGCAG 1 cut(s) 639
PsuI RGATCY 1 cut(s) 541
PsyI GACNNNGTC 1 cut(s) 58
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 2 cut(s) 422, 434
RsaNI GTAC 2 cut(s) 421, 433
RseI CAYNNNNRTG 1 cut(s) 360
SapI GCTCTTC 1 cut(s) 47
SaqAI TTAA 7 cut(s) 327, 666, 705, 764, 794, 929, 1166
SatI GCNGC 9 cut(s) 176, 179, 256, 505, 693, 723, 738, 813, 830
Sau3AI GATC 3 cut(s) 472, 541, 1102
Sau96I GGNCC 5 cut(s) 351, 396, 502, 535, 1071
SchI GAGTC 1 cut(s) 809
ScrFI CCNGG 5 cut(s) 301, 308, 509, 538, 539
SfaNI GCATC 1 cut(s) 799
SfcI CTRYAG 2 cut(s) 635, 1170
SfoI GGCGCC 1 cut(s) 619
SinI GGWCC 2 cut(s) 351, 1071
SmaI CCCGGG 1 cut(s) 539
SmiI ATTTAAAT 1 cut(s) 795
SmiMI CAYNNNNRTG 1 cut(s) 360
Sse9I AATT 5 cut(s) 18, 630, 1062, 1078, 1114
SspDI GGCGCC 1 cut(s) 617
SspMI CTAG 3 cut(s) 383, 786, 851
StyD4I CCNGG 5 cut(s) 299, 306, 507, 536, 537
SwaI ATTTAAAT 1 cut(s) 795
TaaI ACNGT 7 cut(s) 60, 362, 373, 702, 1133, 1148, 1174
TaiI ACGT 2 cut(s) 426, 1187
TaqI TCGA 3 cut(s) 130, 513, 1026
TasI AATT 5 cut(s) 18, 630, 1062, 1078, 1114
TauI GCSGC 2 cut(s) 181, 507
TfiI GAWTC 2 cut(s) 205, 683
Tru1I TTAA 7 cut(s) 327, 666, 705, 764, 794, 929, 1166
Tru9I TTAA 7 cut(s) 327, 666, 705, 764, 794, 929, 1166
TscAI CASTG 1 cut(s) 1000
TseFI GTSAC 3 cut(s) 367, 457, 1177
TseI GCWGC 7 cut(s) 175, 255, 692, 722, 737, 812, 829
Tsp45I GTSAC 3 cut(s) 367, 457, 1177
TspDTI ATGAA 1 cut(s) 1024
TspGWI ACGGA 1 cut(s) 345
TspMI CCCGGG 1 cut(s) 537
TspRI CASTG 1 cut(s) 1000
Tth111I GACNNNGTC 1 cut(s) 58
VpaK11BI GGWCC 2 cut(s) 351, 1071
XapI RAATTY 3 cut(s) 18, 630, 1114
XmaI CCCGGG 1 cut(s) 537
XmnI GAANNNNTTC 1 cut(s) 209
XspI CTAG 3 cut(s) 383, 786, 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.